Rh2BG467400

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014615
Physical Location & Seq
Forward (+)
66020405 .. 66020979
575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG467400.1

Sequence Viewer

Length: 486 bp
ATGAGCCGAGTAGAGATTTCGAGTCCCTTCAGCGTGGACTTGGAAGCAGCAGTGAACCACCTGCTGAATTTCCCTGATACTATCCCCACCTACATGTACCCTTCTCGGGCTGCTCATGGAGCCAAAGAGAACCAAGGTGCCTCTACCATTCCGGTCGACATTTTGGACTCCGCCAAGGACTACACGTTCTACTTGGACGTCCCTGGCTTGTCCAAATCTGATATTCAGGTAACTGTTGAAGATGAAAATACTCTGGTGATTCGGAGCAACGGGAAGAGGAAGCGTGAAGATGGAGAGGAGGAAGGGTGCAAGTATGTTAGGCTTGAGAGGAAGGCACCGCAGAAGATGTTGAGGAAGTTCCGATTGCCTGAGAATGCGAATGTTGGAGCCATCACTGCCAAATGCGAAAACGGGGTCCTGACCGTAGTTGTTGAGAAGCTTCCCCCACTTCCCAAGCCCAAAACAGTCGCAGTTAACGTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

161

Amino Acids

17.79

Weight (kDa)

6.74

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 53 - 159 3.7e-20 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014615)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54050 AT1G54050
fragaria_vesca FvH4_6g35450
malus_domestica MD17G1151000.v1.1
prunus_persica Prupe.3G034800_v2.0.a1
pyrus_communis pycom17g14430
rosa_chinensis RchiOBHm_Chr2g0147001
rosa_laevigata RLG00000020237
rosa_multiflora Rmu_co8216844.1_g000001
rosa_roxburghii Rroxscaffold_2G00099770
rosa_rugosa Rorug02G0397900
rosa_samantha Rh2AG454900 Rh2BG467400 Rh2CG442300 Rh2DG476400
rosa_wichuraiana Rw2G037110 Rw2G037210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 69
AatII GACGTC 1 cut(s) 201
Acc36I ACCTGC 1 cut(s) 69
AccB1I GGYRCC 2 cut(s) 137, 334
AccI GTMKAC 1 cut(s) 156
AciI CCGC 2 cut(s) 171, 338
AcsI RAATTY 1 cut(s) 67
AcuI CTGAAG 1 cut(s) 13
AcyI GRCGYC 1 cut(s) 198
AfaI GTAC 1 cut(s) 98
AfiI CCNNNNNNNGG 1 cut(s) 106
AflIII ACRYGT 2 cut(s) 93, 183
AgsI TTSAA 1 cut(s) 239
AjnI CCWGG 1 cut(s) 202
AloI GAACNNNNNNTCC 2 cut(s) 170, 202
AluBI AGCT 1 cut(s) 439
AluI AGCT 1 cut(s) 439
Ama87I CYCGRG 1 cut(s) 105
ApeKI GCWGC 2 cut(s) 47, 110
ApoI RAATTY 1 cut(s) 67
ArsI GACNNNNNNTTYG 2 cut(s) 399, 431
AspS9I GGNCC 1 cut(s) 415
AsuHPI GGTGA 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 105
AvaII GGWCC 1 cut(s) 415
BanI GGYRCC 2 cut(s) 137, 334
BbvI GCAGC 2 cut(s) 59, 97
BccI CCATC 2 cut(s) 284, 398
BciT130I CCWGG 1 cut(s) 204
BfuAI ACCTGC 1 cut(s) 69
BisI GCNGC 2 cut(s) 48, 111
BlsI GCNGC 2 cut(s) 49, 112
Bme1390I CCNGG 1 cut(s) 204
Bme18I GGWCC 1 cut(s) 415
BmeT110I CYCGRG 1 cut(s) 105
BmgT120I GGNCC 1 cut(s) 415
BmiI GGNNCC 5 cut(s) 121, 139, 336, 388, 416
BmrFI CCNGG 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 344
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 3 cut(s) 133, 174, 202
BsaWI WCCGGW 1 cut(s) 151
BsaXI ACNNNNNCTCC 2 cut(s) 290, 320
Bsc4I CCNNNNNNNGG 1 cut(s) 106
BseBI CCWGG 1 cut(s) 204
BseDI CCNNGG 3 cut(s) 133, 174, 202
BseLI CCNNNNNNNGG 1 cut(s) 106
BseMII CTCAG 1 cut(s) 360
BseRI GAGGAG 1 cut(s) 311
BseXI GCAGC 2 cut(s) 59, 97
Bsh1285I CGRYCG 1 cut(s) 156
BshNI GGYRCC 2 cut(s) 137, 334
BsiEI CGRYCG 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 105
BsiSI CCGG 1 cut(s) 152
BslFI GGGAC 2 cut(s) 9, 185
BslI CCNNNNNNNGG 1 cut(s) 106
BsmFI GGGAC 2 cut(s) 9, 185
BsmI GAATGC 1 cut(s) 379
BsoBI CYCGRG 1 cut(s) 105
BspACI CCGC 2 cut(s) 171, 338
BspCNI CTCAG 1 cut(s) 361
BspLI GGNNCC 5 cut(s) 121, 139, 336, 388, 416
BspMI ACCTGC 1 cut(s) 69
BspT107I GGYRCC 2 cut(s) 137, 334
BssECI CCNNGG 3 cut(s) 133, 174, 202
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 2 cut(s) 133, 174
Bst2UI CCWGG 1 cut(s) 204
Bst4CI ACNGT 3 cut(s) 235, 424, 466
Bst6I CTCTTC 1 cut(s) 269
BstACI GRCGYC 1 cut(s) 198
BstDEI CTNAG 1 cut(s) 369
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 2 cut(s) 119, 395
BstNI CCWGG 1 cut(s) 204
BstNSI RCATGY 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 202
BstV1I GCAGC 2 cut(s) 59, 97
BtsI GCAGTG 2 cut(s) 57, 393
BtsIMutI CAGTG 2 cut(s) 57, 393
BveI ACCTGC 1 cut(s) 69
Cfr13I GGNCC 1 cut(s) 415
Csp6I GTAC 1 cut(s) 97
CviAII CATG 2 cut(s) 94, 116
CviJI RGCY 8 cut(s) 6, 110, 122, 207, 322, 389, 439, 457
CviKI_1 RGCY 8 cut(s) 6, 110, 122, 207, 322, 389, 439, 457
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 1 cut(s) 369
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
EciI GGCGGA 1 cut(s) 160
Eco130I CCWWGG 2 cut(s) 133, 174
Eco47I GGWCC 1 cut(s) 415
Eco57I CTGAAG 1 cut(s) 13
Eco88I CYCGRG 1 cut(s) 105
EcoO109I RGGNCCY 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 202
EcoT14I CCWWGG 2 cut(s) 133, 174
ErhI CCWWGG 2 cut(s) 133, 174
FaeI CATG 2 cut(s) 97, 119
FaiI YATR 3 cut(s) 95, 117, 315
FaqI GGGAC 2 cut(s) 9, 185
FatI CATG 2 cut(s) 93, 115
FblI GTMKAC 1 cut(s) 156
Fnu4HI GCNGC 2 cut(s) 48, 111
Fsp4HI GCNGC 2 cut(s) 48, 111
GluI GCNGC 2 cut(s) 48, 111
HapII CCGG 1 cut(s) 152
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 2 cut(s) 97, 119
HincII GTYRAC 2 cut(s) 157, 475
HindII GTYRAC 2 cut(s) 157, 475
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 3 cut(s) 22, 167, 259
HpaI GTTAAC 1 cut(s) 475
HpaII CCGG 1 cut(s) 152
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 4 cut(s) 37, 55, 157, 475
Hpy188I TCNGA 3 cut(s) 220, 264, 362
Hpy188III TCNNGA 2 cut(s) 418, 483
Hpy8I GTNNAC 4 cut(s) 37, 55, 157, 475
HpyAV CCTTC 4 cut(s) 37, 111, 296, 325
HpyCH4III ACNGT 3 cut(s) 235, 424, 466
HpyCH4IV ACGT 3 cut(s) 185, 198, 477
HpyCH4V TGCA 1 cut(s) 309
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 395
HpyF3I CTNAG 1 cut(s) 369
HpySE526I ACGT 3 cut(s) 185, 198, 477
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 2 cut(s) 97, 119
KspAI GTTAAC 1 cut(s) 475
LmnI GCTCC 3 cut(s) 119, 264, 386
LpnPI CCDG 9 cut(s) 74, 87, 165, 189, 212, 216, 239, 381, 431
Lsp1109I GCAGC 2 cut(s) 59, 97
MaeII ACGT 3 cut(s) 185, 198, 477
MaeIII GTNAC 1 cut(s) 229
MboII GAAGA 4 cut(s) 251, 286, 299, 355
MluCI AATT 1 cut(s) 67
MlyI GAGTC 2 cut(s) 31, 161
MmeI TCCRAC 1 cut(s) 364
MnlI CCTC 6 cut(s) 151, 270, 289, 292, 321, 345
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 1 cut(s) 92
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 1 cut(s) 204
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 1 cut(s) 204
MwoI GCNNNNNNNGC 2 cut(s) 119, 395
NlaIII CATG 2 cut(s) 97, 119
NlaIV GGNNCC 5 cut(s) 121, 139, 336, 388, 416
NmeAIII GCCGAG 1 cut(s) 32
NspI RCATGY 1 cut(s) 97
PaqCI CACCTGC 1 cut(s) 69
PciI ACATGT 1 cut(s) 93
PcsI WCGNNNNNNNCGW 1 cut(s) 474
PctI GAATGC 1 cut(s) 379
PfeI GAWTC 1 cut(s) 259
PkrI GCNGC 2 cut(s) 49, 112
PleI GAGTC 2 cut(s) 30, 161
PpsI GAGTC 2 cut(s) 30, 161
PpuMI RGGWCCY 1 cut(s) 415
PscI ACATGT 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 1 cut(s) 202
PspGI CCWGG 1 cut(s) 202
PspN4I GGNNCC 5 cut(s) 121, 139, 336, 388, 416
PspPI GGNCC 1 cut(s) 415
PspPPI RGGWCCY 1 cut(s) 415
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
RseI CAYNNNNRTG 1 cut(s) 92
SalI GTCGAC 1 cut(s) 155
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 2 cut(s) 48, 111
Sau96I GGNCC 1 cut(s) 415
SchI GAGTC 2 cut(s) 31, 161
ScrFI CCNGG 1 cut(s) 204
SetI ASST 8 cut(s) 63, 92, 139, 188, 201, 231, 441, 480
SinI GGWCC 1 cut(s) 415
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
Sse9I AATT 1 cut(s) 67
SsiI CCGC 2 cut(s) 171, 338
StyD4I CCNGG 1 cut(s) 202
StyI CCWWGG 2 cut(s) 133, 174
TaaI ACNGT 3 cut(s) 235, 424, 466
TaiI ACGT 3 cut(s) 188, 201, 480
TaqI TCGA 2 cut(s) 20, 156
TasI AATT 1 cut(s) 67
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 2 cut(s) 57, 400
TseI GCWGC 2 cut(s) 47, 110
TspDTI ATGAA 1 cut(s) 258
TspRI CASTG 2 cut(s) 57, 400
VpaK11BI GGWCC 1 cut(s) 415
XapI RAATTY 1 cut(s) 67
XceI RCATGY 1 cut(s) 97
XmiI GTMKAC 1 cut(s) 156
ZraI GACGTC 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.