AT1G54820

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010204
Physical Location & Seq
Forward (+)
20447157 .. 20450916
3760 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G54820.3

Sequence Viewer

Length: 1377 bp
ATGAACAACACCAAAATGGAAAACAAAAGCCATAGCAACCACCACCGGTACCATCATCTATCATCCCGGAGCCACGAGCAAAACCAGCGTGGCCTAATTTCGATAAATGCCATAATCATCATTGGCATCTCCATTATCTCCATATTCATAATCCTTGCAATTCTCCTAATAATCATTTTACTTCATCGACTTAAATCCGCAAGAGTCAAAGCACAAGAATTATCTTGCAAAGAAAGCTTCAACAACATGAACAATGGTGGAGCTTCCACCAACTACAGCTACACTTCTAGCCCTGATGACATCAAGAGAGATTGTCTATACTCAAGAAACCCAACATCGTTCAGACAATTACCCCCACAGACAAAAAGTTGTAGGAGAAGCCGAGCCGAAGGAGTAGAAGTGTACACATACAAGGAATTAGAGATTGCAACAAATAATTTCAGCGAGGAGAAGAAAATCGGAAATGGAGATGTGTACAAAGGAGTACTAAGTGATGGAACTGTTGCGGCCATTAAAAAGCTTCATATGTTTAATGATAATGCTAGTAACCAAAAGCATGAAGAACGGTCCTTTAGGCTTGAGGTTGATCTTCTTAGTCGATTACAATGCCCATATTTGGTGGAATTACTTGGATATTGTGCAGATCAAAACCATAGGATCCTGATATATGAATTTATGCCTAATGGTACTGTGGAACATCATCTCCACGATCATAATTTTAAGAATCTAAAGGATCGACCTCAACCATTAGATTGGGGAGCTCGGCTTAGGATCGCCCTTGATTGCGCCAGAGCCCTAGAGTTTCTTCATGAGAATACAATCTCTACTGTTATCCACCGGAACTTCAAGTGTACCAACATTTTGTTGGATCAAAATAATCGAGCTAAAGTTTCAGATTTCGGATTAGCCAAAACCGGATCCGATAAACTAAACGGTGAGATTTCAACAAGGGTTATTGGCACCACAGGATATCTTGCTCCAGAGTATGCCTCTACTGGAAAGCTTACTACAAAATCAGATGTTTATAGTTATGGTATTGTGTTACTACAACTCTTAACGGGTCGCACACCGATCGATTCAAGACGTCCACGGGGACAAGATGTCCTTGTCTCTTGGGCTCTTCCAAGACTAACCAATAGAGAGAAAATTAGTGAAATGGTGGATCCAACCATGAAAGGTCAATACTCACAAAAAGATCTTATTCAGGTAGCAGCCATAGCAGCAGTGTGTGTGCAACCAGAAGCAAGTTATAGACCGTTGATGACGGATGTTGTTCACTCGCTCATTCCCCTTGTTAAAGCTTTCAACAAAAGTACTGATTCTTCTCGGTTTCCTAGCCGTAGAGAAAGCTTGTCATTTGATGATATTATGCCATGA

Protein Analysis

458

Amino Acids

51.87

Weight (kDa)

9.15

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 148 - 328 9.1e-28 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 155 - 328 2.5e-26 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1087
Acc65I GGTACC 1 cut(s) 48
AccB1I GGYRCC 2 cut(s) 48, 959
AciI CCGC 2 cut(s) 198, 506
AclWI GGATC 9 cut(s) 652, 665, 741, 779, 876, 912, 925, 1157, 1170
AcoI YGGCCR 1 cut(s) 507
AcsI RAATTY 1 cut(s) 671
AcyI GRCGYC 1 cut(s) 1084
AfaI GTAC 7 cut(s) 50, 404, 476, 486, 688, 853, 1315
AfiI CCNNNNNNNGG 1 cut(s) 616
AgeI ACCGGT 1 cut(s) 45
AgsI TTSAA 5 cut(s) 241, 847, 945, 1080, 1306
AhdI GACNNNNNGTC 1 cut(s) 1100
AloI GAACNNNNNNTCC 2 cut(s) 687, 719
AluBI AGCT 9 cut(s) 237, 263, 279, 520, 761, 884, 1003, 1301, 1350
AluI AGCT 9 cut(s) 237, 263, 279, 520, 761, 884, 1003, 1301, 1350
Alw21I GWGCWC 1 cut(s) 763
Alw26I GTCTC 1 cut(s) 1114
AlwI GGATC 9 cut(s) 652, 665, 741, 779, 876, 912, 925, 1157, 1170
AoxI GGCC 2 cut(s) 91, 507
ApeKI GCWGC 2 cut(s) 1211, 1220
ApoI RAATTY 1 cut(s) 671
AsiGI ACCGGT 1 cut(s) 45
Asp718I GGTACC 1 cut(s) 48
AspLEI GCGC 1 cut(s) 788
AspS9I GGNCC 1 cut(s) 567
AsuC2I CCSGG 1 cut(s) 67
AsuHPI GGTGA 1 cut(s) 947
AvaII GGWCC 1 cut(s) 567
BamHI GGATCC 3 cut(s) 657, 917, 1162
BanI GGYRCC 2 cut(s) 48, 959
BanII GRGCYC 3 cut(s) 763, 796, 1120
BarI GAAGNNNNNNTAC 2 cut(s) 1306, 1338
BauI CACGAG 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 763
BbvI GCAGC 2 cut(s) 1223, 1232
BccI CCATC 2 cut(s) 60, 488
BceAI ACGGC 1 cut(s) 1323
BcgI CGANNNNNNTGC 4 cut(s) 588, 622, 1054, 1088
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 1114
BfaI CTAG 4 cut(s) 288, 543, 797, 1335
BfmI CTRYAG 1 cut(s) 274
BglII AGATCT 1 cut(s) 1195
BisI GCNGC 3 cut(s) 507, 1212, 1221
BlsI GCNGC 3 cut(s) 508, 1213, 1222
BmcAI AGTACT 2 cut(s) 486, 1315
Bme1390I CCNGG 1 cut(s) 67
Bme18I GGWCC 1 cut(s) 567
BmeRI GACNNNNNGTC 1 cut(s) 1100
BmgT120I GGNCC 1 cut(s) 567
BmiI GGNNCC 6 cut(s) 50, 71, 659, 919, 961, 1164
BmrFI CCNGG 1 cut(s) 67
BmsI GCATC 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 974, 1006
BpmI CTGGAG 1 cut(s) 963
Bpu10I CCTNAGC 1 cut(s) 767
BpuEI CTTGAG 2 cut(s) 307, 599
BpuMI CCSGG 1 cut(s) 67
Bsa29I ATCGAT 1 cut(s) 1074
BsaHI GRCGYC 1 cut(s) 1084
BsaJI CCNNGG 1 cut(s) 1088
BsaWI WCCGGW 3 cut(s) 45, 837, 914
BsaXI ACNNNNNCTCC 4 cut(s) 384, 414, 687, 717
Bsc4I CCNNNNNNNGG 1 cut(s) 616
Bse118I RCCGGY 1 cut(s) 45
Bse1I ACTGG 1 cut(s) 1000
BseCI ATCGAT 1 cut(s) 1074
BseDI CCNNGG 1 cut(s) 1088
BseGI GGATG 2 cut(s) 62, 1273
BseLI CCNNNNNNNGG 1 cut(s) 616
BseNI ACTGG 1 cut(s) 1000
BseRI GAGGAG 1 cut(s) 461
BseXI GCAGC 2 cut(s) 1223, 1232
BsgI GTGCAG 1 cut(s) 660
Bsh1285I CGRYCG 1 cut(s) 1074
BshFI GGCC 2 cut(s) 93, 509
BshNI GGYRCC 2 cut(s) 48, 959
BshTI ACCGGT 1 cut(s) 45
BshVI ATCGAT 1 cut(s) 1074
BsiEI CGRYCG 1 cut(s) 1074
BsiHKAI GWGCWC 1 cut(s) 763
BsiSI CCGG 4 cut(s) 46, 67, 838, 915
BslFI GGGAC 1 cut(s) 1107
BslI CCNNNNNNNGG 1 cut(s) 616
BsmAI GTCTC 1 cut(s) 1114
BsmFI GGGAC 1 cut(s) 1107
BsnI GGCC 2 cut(s) 93, 509
Bsp1286I GDGCHC 3 cut(s) 763, 796, 1120
Bsp1407I TGTACA 2 cut(s) 402, 474
BspACI CCGC 2 cut(s) 198, 506
BspANI GGCC 2 cut(s) 93, 509
BspDI ATCGAT 1 cut(s) 1074
BspHI TCATGA 1 cut(s) 808
BspLI GGNNCC 6 cut(s) 50, 71, 659, 919, 961, 1164
BspPI GGATC 9 cut(s) 652, 665, 741, 779, 876, 912, 925, 1157, 1170
BspQI GCTCTTC 1 cut(s) 1125
BspT107I GGYRCC 2 cut(s) 48, 959
BsrFI RCCGGY 1 cut(s) 45
BsrGI TGTACA 2 cut(s) 402, 474
BsrI ACTGG 1 cut(s) 1000
BssAI RCCGGY 1 cut(s) 45
BssECI CCNNGG 1 cut(s) 1088
BssNI GRCGYC 1 cut(s) 1084
BssSI CACGAG 1 cut(s) 74
Bst2BI CACGAG 1 cut(s) 74
Bst4CI ACNGT 6 cut(s) 502, 567, 691, 829, 935, 1257
Bst6I CTCTTC 1 cut(s) 1125
BstACI GRCGYC 1 cut(s) 1084
BstAUI TGTACA 2 cut(s) 402, 474
BstDEI CTNAG 3 cut(s) 488, 593, 767
BstDSI CCRYGG 1 cut(s) 1088
BstF5I GGATG 2 cut(s) 62, 1273
BstHHI GCGC 1 cut(s) 788
BstMAI GTCTC 1 cut(s) 1114
BstMCI CGRYCG 1 cut(s) 1074
BstMWI GCNNNNNNNGC 4 cut(s) 85, 234, 1217, 1220
BstSCI CCNGG 1 cut(s) 65
BstSFI CTRYAG 1 cut(s) 274
BstV1I GCAGC 2 cut(s) 1223, 1232
BstX2I RGATCY 4 cut(s) 657, 917, 1162, 1195
BstXI CCANNNNNNTGG 1 cut(s) 753
BstYI RGATCY 4 cut(s) 657, 917, 1162, 1195
Bsu15I ATCGAT 1 cut(s) 1074
BsuRI GGCC 2 cut(s) 93, 509
BsuTUI ATCGAT 1 cut(s) 1074
BtgI CCRYGG 1 cut(s) 1088
BtsCI GGATG 2 cut(s) 62, 1273
BtsI GCAGTG 1 cut(s) 1230
BtsIMutI CAGTG 1 cut(s) 1230
CciI TCATGA 1 cut(s) 808
CfoI GCGC 1 cut(s) 788
Cfr10I RCCGGY 1 cut(s) 45
Cfr13I GGNCC 1 cut(s) 567
ClaI ATCGAT 1 cut(s) 1074
Csp6I GTAC 7 cut(s) 49, 403, 475, 485, 687, 852, 1314
CspAI ACCGGT 1 cut(s) 45
CviAII CATG 5 cut(s) 247, 557, 809, 1171, 1374
CviQI GTAC 7 cut(s) 49, 403, 475, 485, 687, 852, 1314
DdeI CTNAG 3 cut(s) 488, 593, 767
DriI GACNNNNNGTC 1 cut(s) 1100
EaeI YGGCCR 1 cut(s) 507
Eam1104I CTCTTC 1 cut(s) 1125
Eam1105I GACNNNNNGTC 1 cut(s) 1100
EarI CTCTTC 1 cut(s) 1125
Ecl136II GAGCTC 1 cut(s) 761
Eco24I GRGCYC 3 cut(s) 763, 796, 1120
Eco32I GATATC 1 cut(s) 971
Eco47I GGWCC 1 cut(s) 567
Eco53kI GAGCTC 1 cut(s) 761
EcoICRI GAGCTC 1 cut(s) 761
EcoRV GATATC 1 cut(s) 971
EcoT38I GRGCYC 3 cut(s) 763, 796, 1120
FaeI CATG 5 cut(s) 250, 560, 812, 1174, 1377
FalI AAGNNNNNCTT 2 cut(s) 1089, 1121
FaqI GGGAC 1 cut(s) 1107
FatI CATG 5 cut(s) 246, 556, 808, 1170, 1373
FauNDI CATATG 1 cut(s) 525
Fnu4HI GCNGC 3 cut(s) 507, 1212, 1221
FokI GGATG 2 cut(s) 49, 1280
FriOI GRGCYC 3 cut(s) 763, 796, 1120
Fsp4HI GCNGC 3 cut(s) 507, 1212, 1221
FspBI CTAG 4 cut(s) 288, 543, 797, 1335
GlaI GCGC 1 cut(s) 787
GluI GCNGC 3 cut(s) 507, 1212, 1221
GsuI CTGGAG 1 cut(s) 963
HaeIII GGCC 2 cut(s) 93, 509
HapII CCGG 4 cut(s) 46, 67, 838, 915
HhaI GCGC 1 cut(s) 788
Hin1I GRCGYC 1 cut(s) 1084
Hin1II CATG 5 cut(s) 250, 560, 812, 1174, 1377
Hin6I GCGC 1 cut(s) 786
HinP1I GCGC 1 cut(s) 786
HindIII AAGCTT 5 cut(s) 235, 518, 1001, 1299, 1348
HinfI GANTC 4 cut(s) 204, 724, 1076, 1319
HpaII CCGG 4 cut(s) 46, 67, 838, 915
HphI GGTGA 1 cut(s) 947
Hpy166II GTNNAC 6 cut(s) 403, 405, 475, 852, 1088, 1276
Hpy188I TCNGA 6 cut(s) 344, 461, 895, 902, 922, 1018
Hpy188III TCNNGA 6 cut(s) 304, 324, 661, 809, 980, 1080
Hpy8I GTNNAC 6 cut(s) 403, 405, 475, 852, 1088, 1276
HpyAV CCTTC 1 cut(s) 383
HpyCH4III ACNGT 6 cut(s) 502, 567, 691, 829, 935, 1257
HpyCH4IV ACGT 1 cut(s) 1084
HpyCH4V TGCA 5 cut(s) 158, 228, 428, 641, 1234
HpyF10VI GCNNNNNNNGC 4 cut(s) 85, 234, 1217, 1220
HpyF3I CTNAG 3 cut(s) 488, 593, 767
HpySE526I ACGT 1 cut(s) 1084
Hsp92I GRCGYC 1 cut(s) 1084
Hsp92II CATG 5 cut(s) 250, 560, 812, 1174, 1377
HspAI GCGC 1 cut(s) 786
KpnI GGTACC 1 cut(s) 52
LguI GCTCTTC 1 cut(s) 1125
LmnI GCTCC 4 cut(s) 69, 260, 758, 982
Lsp1109I GCAGC 2 cut(s) 1223, 1232
LweI GCATC 1 cut(s) 135
MaeI CTAG 4 cut(s) 288, 543, 797, 1335
MaeII ACGT 1 cut(s) 1084
MaeIII GTNAC 2 cut(s) 545, 1041
MboII GAAGA 6 cut(s) 463, 572, 581, 797, 1112, 1314
MflI RGATCY 4 cut(s) 657, 917, 1162, 1195
MhlI GDGCHC 3 cut(s) 763, 796, 1120
MlyI GAGTC 1 cut(s) 213
MmeI TCCRAC 2 cut(s) 846, 1190
MnlI CCTC 4 cut(s) 439, 574, 750, 1000
MseI TTAA 6 cut(s) 192, 513, 531, 720, 1055, 1296
MslI CAYNNNNRTG 1 cut(s) 14
MspI CCGG 4 cut(s) 46, 67, 838, 915
MspR9I CCNGG 1 cut(s) 67
MwoI GCNNNNNNNGC 4 cut(s) 85, 234, 1217, 1220
NciI CCSGG 1 cut(s) 67
NdeI CATATG 1 cut(s) 525
NlaIII CATG 5 cut(s) 250, 560, 812, 1174, 1377
NlaIV GGNNCC 6 cut(s) 50, 71, 659, 919, 961, 1164
NmeAIII GCCGAG 2 cut(s) 407, 742
PagI TCATGA 1 cut(s) 808
PciSI GCTCTTC 1 cut(s) 1125
PfeI GAWTC 3 cut(s) 724, 1076, 1319
PfoI TCCNGGA 1 cut(s) 65
PinAI ACCGGT 1 cut(s) 45
PkrI GCNGC 3 cut(s) 508, 1213, 1222
Ple19I CGATCG 1 cut(s) 1074
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Psp124BI GAGCTC 1 cut(s) 763
PspN4I GGNNCC 6 cut(s) 50, 71, 659, 919, 961, 1164
PspPI GGNCC 1 cut(s) 567
PsuI RGATCY 4 cut(s) 657, 917, 1162, 1195
PvuI CGATCG 1 cut(s) 1074
RsaI GTAC 7 cut(s) 50, 404, 476, 486, 688, 853, 1315
RsaNI GTAC 7 cut(s) 49, 403, 475, 485, 687, 852, 1314
RseI CAYNNNNRTG 1 cut(s) 14
SacI GAGCTC 1 cut(s) 763
SapI GCTCTTC 1 cut(s) 1125
SaqAI TTAA 6 cut(s) 192, 513, 531, 720, 1055, 1296
SatI GCNGC 3 cut(s) 507, 1212, 1221
Sau96I GGNCC 1 cut(s) 567
ScaI AGTACT 2 cut(s) 486, 1315
SchI GAGTC 1 cut(s) 213
ScrFI CCNGG 1 cut(s) 67
SduI GDGCHC 3 cut(s) 763, 796, 1120
SfaNI GCATC 1 cut(s) 135
SfcI CTRYAG 1 cut(s) 274
SinI GGWCC 1 cut(s) 567
SmiMI CAYNNNNRTG 1 cut(s) 14
SmlI CTYRAG 2 cut(s) 322, 578
SmoI CTYRAG 2 cut(s) 322, 578
SsiI CCGC 2 cut(s) 198, 506
SspMI CTAG 4 cut(s) 288, 543, 797, 1335
SstI GAGCTC 1 cut(s) 763
StyD4I CCNGG 1 cut(s) 65
TaaI ACNGT 6 cut(s) 502, 567, 691, 829, 935, 1257
TaiI ACGT 1 cut(s) 1087
TaqI TCGA 6 cut(s) 101, 187, 598, 736, 880, 1074
TatI WGTACW 4 cut(s) 402, 474, 484, 1313
TauI GCSGC 1 cut(s) 509
TfiI GAWTC 3 cut(s) 724, 1076, 1319
Tru1I TTAA 6 cut(s) 192, 513, 531, 720, 1055, 1296
Tru9I TTAA 6 cut(s) 192, 513, 531, 720, 1055, 1296
TscAI CASTG 1 cut(s) 1230
TseI GCWGC 2 cut(s) 1211, 1220
TspDTI ATGAA 9 cut(s) 17, 136, 173, 263, 512, 573, 684, 797, 1187
TspGWI ACGGA 1 cut(s) 1280
TspRI CASTG 1 cut(s) 1230
VpaK11BI GGWCC 1 cut(s) 567
XapI RAATTY 1 cut(s) 671
XcmI CCANNNNNNNNNTGG 1 cut(s) 862
XspI CTAG 4 cut(s) 288, 543, 797, 1335
ZraI GACGTC 1 cut(s) 1085
ZrmI AGTACT 2 cut(s) 486, 1315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.