AT1G55740

Raffinose synthase or seed imbibition protein Sip1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012127
Physical Location & Seq
Reverse (-)
20835256 .. 20838778
3523 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G55740.1

Sequence Viewer

Length: 2265 bp
ATGACCGTTGGTGCCGGAATCAGCGTCACCGACTCAGATTTGGTGGTGCTAGGTCACCGTGTTCTTCACGGCGTTCCGGAGAATGTCTTAGTTACCCCAGCTTCGGGAAACGCCTTAATCGACGGCGCGTTCATCGGCGTCACGTCAGATCAAACCGGAAGCCACCGTGTCTTCTCCCTTGGCAAACTCGAAGACCTAAGGTTTATGTGTGTCTTCCGGTTTAAATTATGGTGGATGACTCAAAGAATGGGGACTAATGGGAAAGAGATTCCTTGTGAGACTCAGTTTCTAATCGTAGAAGCAAACCAAGGTTCTGATTTAGGAGGAAGAGATCAATCTTCTTCGTACGTTGTATTCTTACCTATACTTGAAGGAGATTTTAGAGCTGTTCTTCAAGGGAACGAGGCTAATGAGCTAGAGATTTGTCTTGAAAGTGGTGATCCAACCGTTGACCAATTCGAAGGGAGTCATTTGGTTTTTGTGGCTGCTGGCTCCGACCCTTTTGATGTCATTACCAAAGCTGTCAAGGCTGTTGAACAACATCTTCAAACATTTTCACACCGAGAGAGAAAGAAAATGCCAGATATGTTGAATTGGTTTGGTTGGTGCACTTGGGATGCATTCTATACCAATGTCACAGCCAAAGACGTCAAGCAAGGACTTGAGAGCCTCAAAGCTGGTGGGGTTACCCCAAAGTTTGTCATAATTGATGATGGATGGCAATCTGTTGGCATGGATGAGACCAGTGTAGAATTCAACGCTGATAATGCTGCCAATTTTGCAAACCGGTTAACTCACATCAAGGAGAATCATAAGTTTCAAAAGGATGGCAAAGAAGGCCACCGGGTGGATGACCCTTCATTGAGTCTTGGACATGTTATCACAGACATTAAGAGTAATAATTCGCTCAAGTATGTTTATGTTTGGCATGCGATAACAGGATATTGGGGAGGAGTCAAACCAGGTGTTTCTGGTATGGAACACTACGAATCCAAGGTTGCGTATCCAGTTTCTTCACCAGGAGTTATGTCCAGTGAGAATTGTGGATGTTTAGAAAGCATAACTAAGAATGGACTTGGTTTGGTGAATCCTGAGAAAGTCTTTAGCTTCTATAATGATCTTCACTCATACCTTGCATCCGTTGGGGTCGATGGTGTCAAAGTAGACGTCCAAAACATTCTTGAAACTCTTGGAGCTGGACATGGTGGTCGTGTCAAACTTGCAAAGAAGTATCACCAGGCTTTGGAAGCTTCTATTTCAAGAAACTTCCCTGACAATGGTATTATCTCTTGCATGAGTCATAACACAGATGGTCTCTACAGCGCAAAAAAGACTGCTGTTATAAGAGCATCAGACGATTTCTGGCCTAGAGACCCCGCTTCACACACGATCCACATTGCTTCTGTTGCATATAACACACTTTTCCTTGGAGAGTTTATGCAGCCGGACTGGGACATGTTTCATAGTTTGCATCCGATGGCTGAATATCATGCTGCAGCTCGCGCGGTTGGAGGATGTGCCATATATGTCAGCGACAAACCGGGACAACATGACTTCAACCTTTTAAGAAAGCTAGTACTTCGAGACGGTTCCATACTCAGAGCAAAGCTTCCCGGAAGACCAACCAGTGATTGCTTCTTCAGTGATCCAGTCAGAGACAATAAAAGTCTTCTGAAAATATGGAACCTGAACGAATTCACTGGCGTTATTGGAGTTTTCAACTGCCAAGGCGCTGGATGGTGTAAGAACGAGAAGAGATACCTAATCCATGACCAAGAACCTGGGACCATTTCTGGTTGTGTCCGAACCAATGATGTTCATTATCTTCATAAAGTAGCAGCCTTTGAATGGACAGGAGATTCCATTGTCTATTCCCATCTCAGAGGAGAGTTAGTGTATCTCCCAAAAGATACATCTTTACCAGTGACATTGATGCCACGTGAATACGAAGTTTTCACGGTGGTTCCGGTGAAGGAATTTTCCGATGGAAGTAAATTTGCACCGGTGGGGCTTATGGAGATGTTTAATTCTGGAGGAGCCATTGTGAGTTTAAGATACGATGACGAGGGGACTAAATTTGTCGTTAGGATGAAACTTCGAGGCAGTGGTTTGGTTGGTGTTTACTCGTCCGTTCGACGACCACGAAGTGTTACGGTGGATTCAGACGACGTGGAGTACCGGTATGAACCGGAATCCGGTTTAGTTACGTTTACTTTAGGAGTTCCGGAGAAAGAATTGTATCTGTGGGACGTGGTCATCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

754

Amino Acids

83.48

Weight (kDa)

5.76

Isoelectric Point (pI)

28.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 7 - 738 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012127)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55740
fragaria_vesca FvH4_3g41930 FvH4_3g41930 FvH4_3g41930
malus_domestica MD03G1040100.v1.1 MD11G1041500.v1.1
prunus_persica Prupe.6G032400_v2.0.a1 Prupe.6G032400_v2.0.a1
pyrus_communis pycom03g03080 pycom11g03510
rosa_chinensis RchiOBHm_Chr5g0075141
rosa_laevigata RLG00000036533
rosa_multiflora Rmu_ssc0000217.1_g000009
rosa_roxburghii Rroxscaffold_1G00006480
rosa_rugosa Rorug05G0436500
rosa_samantha Rh5AG493800 Rh5CG539100 Rh5DG527900
rosa_wichuraiana Rw5G045810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1343
AasI GACNNNNNNGTC 1 cut(s) 1206
AatII GACGTC 2 cut(s) 651, 1170
AccB1I GGYRCC 1 cut(s) 11
AccB7I CCANNNNNTGG 2 cut(s) 847, 1243
AccI GTMKAC 1 cut(s) 1164
AccII CGCG 3 cut(s) 128, 1503, 1505
AccIII TCCGGA 2 cut(s) 76, 2224
AciI CCGC 2 cut(s) 1377, 1505
AclWI GGATC 3 cut(s) 434, 1384, 1640
AcsI RAATTY 5 cut(s) 752, 1694, 1976, 1994, 2075
AcuI CTGAAG 1 cut(s) 1624
AcvI CACGTG 1 cut(s) 1940
AcyI GRCGYC 3 cut(s) 138, 648, 1167
AdeI CACNNNGTG 2 cut(s) 847, 2147
AfaI GTAC 3 cut(s) 347, 1578, 2177
AfiI CCNNNNNNNGG 7 cut(s) 103, 104, 847, 1243, 1277, 1848, 2195
AflIII ACRYGT 2 cut(s) 874, 1455
AgeI ACCGGT 3 cut(s) 786, 2002, 2178
AjiI CACGTC 3 cut(s) 144, 2170, 2251
AjnI CCWGG 4 cut(s) 961, 1018, 1236, 1780
Alw21I GWGCWC 1 cut(s) 611
Alw26I GTCTC 6 cut(s) 272, 734, 1319, 1365, 1578, 1650
Alw44I GTGCAC 1 cut(s) 607
AlwI GGATC 3 cut(s) 434, 1384, 1640
Aor13HI TCCGGA 2 cut(s) 76, 2224
AoxI GGCC 2 cut(s) 838, 1364
ApaLI GTGCAC 1 cut(s) 607
ApeKI GCWGC 6 cut(s) 485, 770, 1441, 1493, 1496, 1838
ApoI RAATTY 5 cut(s) 752, 1694, 1976, 1994, 2075
ArsI GACNNNNNNTTYG 2 cut(s) 1152, 1184
AsiGI ACCGGT 3 cut(s) 786, 2002, 2178
Asp700I GAANNNNTTC 1 cut(s) 1694
AspLEI GCGC 4 cut(s) 128, 1325, 1505, 1733
AspS9I GGNCC 1 cut(s) 1785
AsuC2I CCSGG 3 cut(s) 845, 1542, 1614
AsuHPI GGTGA 7 cut(s) 19, 47, 449, 1008, 1096, 1226, 1981
AsuII TTCGAA 1 cut(s) 459
AvaII GGWCC 1 cut(s) 1785
AxyI CCTNAGG 1 cut(s) 197
BaeGI GKGCMC 1 cut(s) 611
BanI GGYRCC 1 cut(s) 11
BbrPI CACGTG 1 cut(s) 1940
BbsI GAAGAC 5 cut(s) 163, 198, 205, 1624, 1661
Bbv12I GWGCWC 1 cut(s) 611
BbvI GCAGC 6 cut(s) 472, 757, 1453, 1480, 1508, 1850
BccI CCATC 9 cut(s) 707, 711, 821, 1145, 1304, 1471, 1731, 1884, 1979
BceAI ACGGC 2 cut(s) 85, 139
BciT130I CCWGG 4 cut(s) 963, 1020, 1238, 1782
BciVI GTATCC 1 cut(s) 1014
BcnI CCSGG 3 cut(s) 845, 1542, 1614
BcoDI GTCTC 6 cut(s) 272, 734, 1319, 1365, 1578, 1650
BfaI CTAG 4 cut(s) 50, 416, 1368, 1574
BfmI CTRYAG 2 cut(s) 1318, 1494
BfoI RGCGCY 1 cut(s) 1734
BfuI GTATCC 1 cut(s) 1014
BisI GCNGC 6 cut(s) 486, 771, 1442, 1494, 1497, 1839
BlsI GCNGC 6 cut(s) 487, 772, 1443, 1495, 1498, 1840
BmcAI AGTACT 1 cut(s) 1578
Bme1390I CCNGG 7 cut(s) 845, 963, 1020, 1238, 1542, 1614, 1782
Bme18I GGWCC 1 cut(s) 1785
BmgBI CACGTC 3 cut(s) 144, 2170, 2251
BmgT120I GGNCC 1 cut(s) 1785
BmiI GGNNCC 7 cut(s) 13, 493, 1591, 1685, 1786, 1965, 2038
BmrFI CCNGG 7 cut(s) 845, 963, 1020, 1238, 1542, 1614, 1782
BmrI ACTGGG 1 cut(s) 1459
BmsI GCATC 5 cut(s) 607, 1145, 1358, 1480, 1923
BmuI ACTGGG 1 cut(s) 1459
BpiI GAAGAC 5 cut(s) 163, 198, 205, 1624, 1661
BpmI CTGGAG 1 cut(s) 2052
Bpu14I TTCGAA 1 cut(s) 459
BpuEI CTTGAG 2 cut(s) 683, 893
BpuMI CCSGG 3 cut(s) 845, 1542, 1614
BsaAI YACGTR 1 cut(s) 1940
BsaBI GATNNNNATC 1 cut(s) 721
BsaHI GRCGYC 3 cut(s) 138, 648, 1167
BsaI GGTCTC 3 cut(s) 734, 1319, 1365
BsaJI CCNNGG 6 cut(s) 178, 307, 993, 1426, 1726, 1781
BsaXI ACNNNNNCTCC 6 cut(s) 1878, 1908, 2028, 2058, 2165, 2195
Bsc4I CCNNNNNNNGG 7 cut(s) 103, 104, 847, 1243, 1277, 1848, 2195
Bse118I RCCGGY 3 cut(s) 786, 2002, 2178
Bse1I ACTGG 8 cut(s) 744, 1007, 1032, 1454, 1626, 1649, 1705, 1922
Bse21I CCTNAGG 1 cut(s) 197
Bse3DI GCAATG 1 cut(s) 1395
Bse8I GATNNNNATC 1 cut(s) 721
BseAI TCCGGA 2 cut(s) 76, 2224
BseBI CCWGG 4 cut(s) 963, 1020, 1238, 1782
BseDI CCNNGG 6 cut(s) 178, 307, 993, 1426, 1726, 1781
BseJI GATNNNNATC 1 cut(s) 721
BseLI CCNNNNNNNGG 7 cut(s) 103, 104, 847, 1243, 1277, 1848, 2195
BseMI GCAATG 1 cut(s) 1395
BseMII CTCAG 5 cut(s) 48, 296, 1083, 1612, 1894
BseNI ACTGG 8 cut(s) 744, 1007, 1032, 1454, 1626, 1649, 1705, 1922
BseRI GAGGAG 3 cut(s) 966, 1899, 2049
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 6 cut(s) 472, 757, 1453, 1480, 1508, 1850
BseYI CCCAGC 1 cut(s) 97
Bsh1236I CGCG 3 cut(s) 128, 1503, 1505
BshFI GGCC 2 cut(s) 840, 1366
BshNI GGYRCC 1 cut(s) 11
BshTI ACCGGT 3 cut(s) 786, 2002, 2178
BsiHKAI GWGCWC 1 cut(s) 611
BsiWI CGTACG 1 cut(s) 345
BslFI GGGAC 6 cut(s) 265, 1466, 1557, 1798, 2083, 2261
BslI CCNNNNNNNGG 7 cut(s) 103, 104, 847, 1243, 1277, 1848, 2195
BsmAI GTCTC 6 cut(s) 272, 734, 1319, 1365, 1578, 1650
BsmBI CGTCTC 1 cut(s) 1578
BsmFI GGGAC 6 cut(s) 265, 1466, 1557, 1798, 2083, 2261
BsmI GAATGC 1 cut(s) 620
BsnI GGCC 2 cut(s) 840, 1366
Bso31I GGTCTC 3 cut(s) 734, 1319, 1365
Bsp119I TTCGAA 1 cut(s) 459
Bsp1286I GDGCHC 1 cut(s) 611
Bsp13I TCCGGA 2 cut(s) 76, 2224
Bsp143I GATC 6 cut(s) 148, 331, 439, 1117, 1389, 1645
BspACI CCGC 2 cut(s) 1377, 1505
BspANI GGCC 2 cut(s) 840, 1366
BspCNI CTCAG 5 cut(s) 47, 295, 1084, 1611, 1893
BspEI TCCGGA 2 cut(s) 76, 2224
BspFNI CGCG 3 cut(s) 128, 1503, 1505
BspLI GGNNCC 7 cut(s) 13, 493, 1591, 1685, 1786, 1965, 2038
BspMAI CTGCAG 1 cut(s) 1498
BspPI GGATC 3 cut(s) 434, 1384, 1640
BspT104I TTCGAA 1 cut(s) 459
BspT107I GGYRCC 1 cut(s) 11
BspTNI GGTCTC 3 cut(s) 734, 1319, 1365
BsrDI GCAATG 1 cut(s) 1395
BsrFI RCCGGY 3 cut(s) 786, 2002, 2178
BsrI ACTGG 8 cut(s) 744, 1007, 1032, 1454, 1626, 1649, 1705, 1922
BssAI RCCGGY 3 cut(s) 786, 2002, 2178
BssECI CCNNGG 6 cut(s) 178, 307, 993, 1426, 1726, 1781
BssMI GATC 6 cut(s) 148, 331, 439, 1117, 1389, 1645
BssNI GRCGYC 3 cut(s) 138, 648, 1167
BssT1I CCWWGG 5 cut(s) 178, 307, 993, 1426, 1726
Bst2UI CCWGG 4 cut(s) 963, 1020, 1238, 1782
Bst4CI ACNGT 7 cut(s) 7, 59, 167, 448, 1589, 1960, 2155
Bst6I CTCTTC 2 cut(s) 322, 1748
BstACI GRCGYC 3 cut(s) 138, 648, 1167
BstBAI YACGTR 1 cut(s) 1940
BstBI TTCGAA 1 cut(s) 459
BstC8I GCNNGC 3 cut(s) 490, 930, 1501
BstDEI CTNAG 8 cut(s) 34, 88, 197, 282, 1065, 1092, 1598, 1880
BstEII GGTNACC 2 cut(s) 53, 685
BstFNI CGCG 3 cut(s) 128, 1503, 1505
BstH2I RGCGCY 1 cut(s) 1734
BstHHI GCGC 4 cut(s) 128, 1325, 1505, 1733
BstKTI GATC 6 cut(s) 151, 334, 442, 1120, 1392, 1648
BstMAI GTCTC 6 cut(s) 272, 734, 1319, 1365, 1578, 1650
BstMBI GATC 6 cut(s) 148, 331, 439, 1117, 1389, 1645
BstMWI GCNNNNNNNGC 7 cut(s) 527, 767, 779, 837, 1247, 1406, 1502
BstNI CCWGG 4 cut(s) 963, 1020, 1238, 1782
BstNSI RCATGY 3 cut(s) 878, 932, 1459
BstPI GGTNACC 2 cut(s) 53, 685
BstSCI CCNGG 7 cut(s) 843, 961, 1018, 1236, 1540, 1612, 1780
BstSFI CTRYAG 2 cut(s) 1318, 1494
BstSLI GKGCMC 1 cut(s) 611
BstUI CGCG 3 cut(s) 128, 1503, 1505
BstV1I GCAGC 6 cut(s) 472, 757, 1453, 1480, 1508, 1850
BstV2I GAAGAC 5 cut(s) 163, 198, 205, 1624, 1661
BstXI CCANNNNNNTGG 2 cut(s) 1733, 1781
Bsu36I CCTNAGG 1 cut(s) 197
BsuI GTATCC 1 cut(s) 1014
BsuRI GGCC 2 cut(s) 840, 1366
BtrI CACGTC 3 cut(s) 144, 2170, 2251
BtsI GCAGTG 1 cut(s) 2110
BtsIMutI CAGTG 7 cut(s) 751, 1039, 1633, 1648, 1698, 1929, 2110
Cac8I GCNNGC 3 cut(s) 490, 930, 1501
CfoI GCGC 4 cut(s) 128, 1325, 1505, 1733
Cfr10I RCCGGY 3 cut(s) 786, 2002, 2178
Cfr13I GGNCC 1 cut(s) 1785
CseI GACGC 2 cut(s) 13, 127
CsiI ACCWGGT 1 cut(s) 961
Csp6I GTAC 3 cut(s) 346, 1577, 2176
CspAI ACCGGT 3 cut(s) 786, 2002, 2178
CspCI CAANNNNNGTGG 2 cut(s) 661, 696
CviAII CATG 9 cut(s) 733, 875, 929, 1202, 1294, 1456, 1490, 1550, 1769
CviQI GTAC 3 cut(s) 346, 1577, 2176
DdeI CTNAG 8 cut(s) 34, 88, 197, 282, 1065, 1092, 1598, 1880
DpnI GATC 6 cut(s) 150, 333, 441, 1119, 1391, 1647
DpnII GATC 6 cut(s) 148, 331, 439, 1117, 1389, 1645
DraI TTTAAA 1 cut(s) 223
DraIII CACNNNGTG 2 cut(s) 847, 2147
DrdI GACNNNNNNGTC 1 cut(s) 1206
DseDI GACNNNNNNGTC 1 cut(s) 1206
Eam1104I CTCTTC 2 cut(s) 322, 1748
EarI CTCTTC 2 cut(s) 322, 1748
Eco130I CCWWGG 5 cut(s) 178, 307, 993, 1426, 1726
Eco31I GGTCTC 3 cut(s) 734, 1319, 1365
Eco47I GGWCC 1 cut(s) 1785
Eco57I CTGAAG 1 cut(s) 1624
Eco72I CACGTG 1 cut(s) 1940
Eco81I CCTNAGG 1 cut(s) 197
Eco91I GGTNACC 2 cut(s) 53, 685
EcoO65I GGTNACC 2 cut(s) 53, 685
EcoRI GAATTC 2 cut(s) 752, 1694
EcoRII CCWGG 4 cut(s) 961, 1018, 1236, 1780
EcoT14I CCWWGG 5 cut(s) 178, 307, 993, 1426, 1726
EcoT22I ATGCAT 1 cut(s) 622
ErhI CCWWGG 5 cut(s) 178, 307, 993, 1426, 1726
Esp3I CGTCTC 1 cut(s) 1578
FaeI CATG 9 cut(s) 736, 878, 932, 1205, 1297, 1459, 1493, 1553, 1772
FaqI GGGAC 6 cut(s) 265, 1466, 1557, 1798, 2083, 2261
FatI CATG 9 cut(s) 732, 874, 928, 1201, 1293, 1455, 1489, 1549, 1768
FauI CCCGC 1 cut(s) 1384
FblI GTMKAC 1 cut(s) 1164
Fnu4HI GCNGC 6 cut(s) 486, 771, 1442, 1494, 1497, 1839
Fsp4HI GCNGC 6 cut(s) 486, 771, 1442, 1494, 1497, 1839
FspBI CTAG 4 cut(s) 50, 416, 1368, 1574
GlaI GCGC 4 cut(s) 127, 1324, 1504, 1732
GluI GCNGC 6 cut(s) 486, 771, 1442, 1494, 1497, 1839
GsaI CCCAGC 1 cut(s) 101
GsuI CTGGAG 1 cut(s) 2052
HaeII RGCGCY 1 cut(s) 1734
HaeIII GGCC 2 cut(s) 840, 1366
HgaI GACGC 2 cut(s) 13, 127
HhaI GCGC 4 cut(s) 128, 1325, 1505, 1733
Hin1I GRCGYC 3 cut(s) 138, 648, 1167
Hin1II CATG 9 cut(s) 736, 878, 932, 1205, 1297, 1459, 1493, 1553, 1772
Hin6I GCGC 4 cut(s) 126, 1323, 1503, 1731
HinP1I GCGC 4 cut(s) 126, 1323, 1503, 1731
HincII GTYRAC 2 cut(s) 451, 792
HindII GTYRAC 2 cut(s) 451, 792
HindIII AAGCTT 2 cut(s) 1248, 1607
HpaI GTTAAC 1 cut(s) 792
HphI GGTGA 7 cut(s) 19, 47, 449, 1008, 1096, 1226, 1981
Hpy166II GTNNAC 6 cut(s) 451, 609, 792, 1165, 2122, 2211
Hpy188III TCNNGA 9 cut(s) 77, 105, 428, 1091, 1181, 1260, 1583, 2031, 2225
Hpy8I GTNNAC 6 cut(s) 451, 609, 792, 1165, 2122, 2211
Hpy99I CGWCG 3 cut(s) 125, 2139, 2171
HpyAV CCTTC 5 cut(s) 365, 455, 830, 867, 1966
HpyCH4III ACNGT 7 cut(s) 7, 59, 167, 448, 1589, 1960, 2155
HpyCH4IV ACGT 8 cut(s) 143, 348, 648, 1167, 1939, 2169, 2207, 2250
HpyF10VI GCNNNNNNNGC 7 cut(s) 527, 767, 779, 837, 1247, 1406, 1502
HpyF3I CTNAG 8 cut(s) 34, 88, 197, 282, 1065, 1092, 1598, 1880
HpySE526I ACGT 8 cut(s) 143, 348, 648, 1167, 1939, 2169, 2207, 2250
Hsp92I GRCGYC 3 cut(s) 138, 648, 1167
Hsp92II CATG 9 cut(s) 736, 878, 932, 1205, 1297, 1459, 1493, 1553, 1772
HspAI GCGC 4 cut(s) 126, 1323, 1503, 1731
Kpn2I TCCGGA 2 cut(s) 76, 2224
KspAI GTTAAC 1 cut(s) 792
Kzo9I GATC 6 cut(s) 148, 331, 439, 1117, 1389, 1645
LmnI GCTCC 3 cut(s) 497, 1193, 2036
Lsp1109I GCAGC 6 cut(s) 472, 757, 1453, 1480, 1508, 1850
LweI GCATC 5 cut(s) 607, 1145, 1358, 1480, 1923
MabI ACCWGGT 1 cut(s) 961
MaeI CTAG 4 cut(s) 50, 416, 1368, 1574
MaeII ACGT 8 cut(s) 143, 348, 648, 1167, 1939, 2169, 2207, 2250
MaeIII GTNAC 9 cut(s) 25, 53, 91, 139, 634, 685, 1924, 2149, 2203
MalI GATC 6 cut(s) 150, 333, 441, 1119, 1391, 1647
MboI GATC 6 cut(s) 148, 331, 439, 1117, 1389, 1645
MhlI GDGCHC 1 cut(s) 611
MlyI GAGTC 7 cut(s) 26, 232, 274, 475, 874, 963, 1306
MmeI TCCRAC 3 cut(s) 467, 519, 1489
MnlI CCTC 9 cut(s) 317, 397, 680, 944, 1505, 1877, 2027, 2059, 2093
Mph1103I ATGCAT 1 cut(s) 622
MroI TCCGGA 2 cut(s) 76, 2224
MroXI GAANNNNTTC 1 cut(s) 1694
MseI TTAA 7 cut(s) 116, 222, 791, 891, 1565, 2025, 2051
MspR9I CCNGG 7 cut(s) 845, 963, 1020, 1238, 1542, 1614, 1782
Mva1269I GAATGC 1 cut(s) 620
MvaI CCWGG 4 cut(s) 963, 1020, 1238, 1782
MvnI CGCG 3 cut(s) 128, 1503, 1505
MwoI GCNNNNNNNGC 7 cut(s) 527, 767, 779, 837, 1247, 1406, 1502
NciI CCSGG 3 cut(s) 845, 1542, 1614
NdeII GATC 6 cut(s) 148, 331, 439, 1117, 1389, 1645
NlaIII CATG 9 cut(s) 736, 878, 932, 1205, 1297, 1459, 1493, 1553, 1772
NlaIV GGNNCC 7 cut(s) 13, 493, 1591, 1685, 1786, 1965, 2038
NmuCI GTSAC 5 cut(s) 25, 53, 139, 634, 1924
NsiI ATGCAT 1 cut(s) 622
NspI RCATGY 3 cut(s) 878, 932, 1459
NspV TTCGAA 1 cut(s) 459
PaeI GCATGC 1 cut(s) 932
PciI ACATGT 2 cut(s) 874, 1455
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 2140
PctI GAATGC 1 cut(s) 620
PdmI GAANNNNTTC 1 cut(s) 1694
PfeI GAWTC 8 cut(s) 18, 268, 808, 989, 1087, 1859, 2159, 2192
Pfl23II CGTACG 1 cut(s) 345
PflFI GACNNNGTC 1 cut(s) 2252
PflMI CCANNNNNTGG 2 cut(s) 847, 1243
PfoI TCCNGGA 1 cut(s) 1612
PinAI ACCGGT 3 cut(s) 786, 2002, 2178
PkrI GCNGC 6 cut(s) 487, 772, 1443, 1495, 1498, 1840
PleI GAGTC 7 cut(s) 26, 232, 274, 474, 873, 962, 1305
PmaCI CACGTG 1 cut(s) 1940
PmlI CACGTG 1 cut(s) 1940
PpsI GAGTC 7 cut(s) 26, 232, 274, 474, 873, 962, 1305
Ppu21I YACGTR 1 cut(s) 1940
PscI ACATGT 2 cut(s) 874, 1455
PsiI TTATAA 1 cut(s) 1343
Psp6I CCWGG 4 cut(s) 961, 1018, 1236, 1780
PspCI CACGTG 1 cut(s) 1940
PspEI GGTNACC 2 cut(s) 53, 685
PspFI CCCAGC 1 cut(s) 97
PspGI CCWGG 4 cut(s) 961, 1018, 1236, 1780
PspLI CGTACG 1 cut(s) 345
PspN4I GGNNCC 7 cut(s) 13, 493, 1591, 1685, 1786, 1965, 2038
PspPI GGNCC 1 cut(s) 1785
PstI CTGCAG 1 cut(s) 1498
PsyI GACNNNGTC 1 cut(s) 2252
RsaI GTAC 3 cut(s) 347, 1578, 2177
RsaNI GTAC 3 cut(s) 346, 1577, 2176
SaqAI TTAA 7 cut(s) 116, 222, 791, 891, 1565, 2025, 2051
SatI GCNGC 6 cut(s) 486, 771, 1442, 1494, 1497, 1839
Sau3AI GATC 6 cut(s) 148, 331, 439, 1117, 1389, 1645
Sau96I GGNCC 1 cut(s) 1785
ScaI AGTACT 1 cut(s) 1578
SchI GAGTC 7 cut(s) 26, 232, 274, 475, 874, 963, 1306
ScrFI CCNGG 7 cut(s) 845, 963, 1020, 1238, 1542, 1614, 1782
SduI GDGCHC 1 cut(s) 611
SexAI ACCWGGT 1 cut(s) 961
SfaNI GCATC 5 cut(s) 607, 1145, 1358, 1480, 1923
SfcI CTRYAG 2 cut(s) 1318, 1494
SfuI TTCGAA 1 cut(s) 459
SgrAI CRCCGGYG 1 cut(s) 2002
SinI GGWCC 1 cut(s) 1785
SmlI CTYRAG 2 cut(s) 662, 908
SmoI CTYRAG 2 cut(s) 662, 908
SphI GCATGC 1 cut(s) 932
SsiI CCGC 2 cut(s) 1377, 1505
SspMI CTAG 4 cut(s) 50, 416, 1368, 1574
StyD4I CCNGG 7 cut(s) 843, 961, 1018, 1236, 1540, 1612, 1780
StyI CCWWGG 5 cut(s) 178, 307, 993, 1426, 1726
TaaI ACNGT 7 cut(s) 7, 59, 167, 448, 1589, 1960, 2155
TaiI ACGT 8 cut(s) 146, 351, 651, 1170, 1942, 2172, 2210, 2253
TaqI TCGA 7 cut(s) 120, 189, 459, 1149, 1582, 2098, 2134
TatI WGTACW 1 cut(s) 1576
TfiI GAWTC 8 cut(s) 18, 268, 808, 989, 1087, 1859, 2159, 2192
Tru1I TTAA 7 cut(s) 116, 222, 791, 891, 1565, 2025, 2051
Tru9I TTAA 7 cut(s) 116, 222, 791, 891, 1565, 2025, 2051
TscAI CASTG 7 cut(s) 751, 1039, 1633, 1648, 1705, 1929, 2110
TseFI GTSAC 5 cut(s) 25, 53, 139, 634, 1924
TseI GCWGC 6 cut(s) 485, 770, 1441, 1493, 1496, 1838
Tsp45I GTSAC 5 cut(s) 25, 53, 139, 634, 1924
TspDTI ATGAA 7 cut(s) 121, 849, 1451, 1808, 1817, 2105, 2199
TspGWI ACGGA 2 cut(s) 1129, 2119
TspRI CASTG 7 cut(s) 751, 1039, 1633, 1648, 1705, 1929, 2110
Tth111I GACNNNGTC 1 cut(s) 2252
Van91I CCANNNNNTGG 2 cut(s) 847, 1243
VneI GTGCAC 1 cut(s) 607
VpaK11BI GGWCC 1 cut(s) 1785
XapI RAATTY 5 cut(s) 752, 1694, 1976, 1994, 2075
XceI RCATGY 3 cut(s) 878, 932, 1459
XmiI GTMKAC 1 cut(s) 1164
XmnI GAANNNNTTC 1 cut(s) 1694
XspI CTAG 4 cut(s) 50, 416, 1368, 1574
ZraI GACGTC 2 cut(s) 649, 1168
ZrmI AGTACT 1 cut(s) 1578
Zsp2I ATGCAT 1 cut(s) 622
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.