MD03G1040100.v1.1

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012127
Physical Location & Seq
Reverse (-)
3178893 .. 3183329
4437 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1040100.v1.1.491

Sequence Viewer

Length: 2274 bp
ATGACGGTTGGGGCTGGGATTTCTGTGGAGGATGGGAACTTGGTGGTGTTGGGGAACAAAGTGTTGTCTGAGGTTCATGACAACGTTGTGGTTACTCCGGCATCTGGGGGTGCATTGACCAATGGTGCTTTCATCGGTGTCCAATCTGATCAGGTTGGTAGCCGCAGGGTCTTCCCGATTGGCAAACTTGAGGGATTGCGTTTCATGTGCGTTTTCCGGTTTAAATTATGGTGGATGACACAGAGGATGGGCAATAGAGGCCAAGATGTTCCCTTTGAGACTCAGTTTTTGATTGTGGAAACAAAGGATGAGTCTCATTTTGGTGAAGGAAGCAAAGATGGGGCGGATCAGTCTGCAACTTATACCGTTATCTTACCAATTCTCGAAGGGGACTTCAGGGCTGTTCTTCAAGGAAATGAACTTAATGAGATTGAAATCTGCCTAGAAAGTGGAGATCCCGCTGCTGATGGATTTGAGGGTAGTCATTTGGTTTTCATTGGAGCCGGATCAGACCCATTCGATGTCATCACAGATTCTGTGAAGACCGTGGAGAAACATTTGCAGACATTTTCTCATCGCGAGAGAAAGAAGATGCCAGACATGTTGAATTGGTTTGGCTGGTGTACGTGGGATGCTTTCTACACCGATGTGACTTCAGAAGGCCTGAAGCAAGGACTGCAGAGCCTAGAAAGTGGTGGAGCCCCTCCAAAGTTTGTTATTCTTGATGACGGATGGCAGTCAGTCGACATGGATTCCTCTGGTGTTGGATACGACGCTGATAATGCAGCAAACTTTGCAAACAGGTTAACACATATAAAAGAGAACCACAAATTTCAGAAAGATGGTAAAGAGGGTCAGAGAGTAGAGGATCCTGCTTTGGGGCTTCGCCACATTGTTACCGAAATTAAGGAAAAACACGCTTTGAAATATGCTTATGTGTGGCATGCCATAACTGGATATTGGGGCGGTGTTAGACCTGGTGTTGCTGAAATGGAACACTACGATTCAAAGTTGGCCTACCCAATTTCATCTCCAGGAGTTGAGTCTAATGAGGATTGTGATGCCTTGAAAAGTATTACAACAAATGGGCTCGGCCTTGTGAACCCCGAGAAAGTTTTCAACTTTTATGATGAACTGCACTCTTATCTCGCATCAGCTGGTATAGATGGAGTCAAAGTTGATGTTCAGAACATTCTTGAAACCCTCGGGGCAGGCCATGGTGGAAGGGTAAAACTTACGAGAAAATACCATCAAGCATTGGAAGCGTCTGTCGCTAGAAACTTTCCTGACAATGGAATTATTTCTTGTATGAGTCACAATACAGATGCTTTATACAGTGTGAAGCGGACAGCTGTTATAAGGGCATCAGATGATTTCTGGCCTAGAGATCCAGCATCACACACAATTCATATCGCATCGGTTGCTTACAACACCGTTTTCCTTGGGGAGTTTATGCAGCCGGATTGGGATATGTTTCATAGCCTGCATCCAATGGCTGAATATCATGGAGCAGCTCGCGCGGTTGGAGGATGTGCAATTTATGTTAGTGACAAGCCTGGGCAGCATGACTTCGATCTTCTGAGGAAGCTCGTACTTCCTGATGGATCTATCTTGAGGGCCAAACTTCCAGGAAGACCAACAAGGGATTGCTTATTTTCTGATCCTGCCCGAGATGGGAAAAGTCTTCTGAAGATATGGAATCTGAACGATGTTACTGGAGTTGTGGGGGTCTTCAATTGCCAGGGAGCTGGGTGGTGTAAGGTTGGAAAGAAGAACCTCATCCATGACCTGGAACCGGGCACAATTACCGGGGTTATTCGGGCTAAAGATGTTGATTATTTGCCCAAGGTCGCAGATGAGAAATGGTCTGGGGATGTTGTCGTATTTTCTCATCTTGGTGGAGAGGTGTCTTATCTTCCCAAGGATACATCCATGCCAATAACATTAAAATCCCGGGAATATGAAGTCTTCACAGTGGTTCCTGTCAAGGAACTGTCCAACAGCGTAAAATTTGCTCCTATAGGCCTAATCAAGATGTTCAACTCTGGGGGAGCCATAAAAGAATATGATGAACCTAACACAAGCACAACAGTTGTCGTGAAAGCTCGCGGATGTGGTATATTCGGAGCCTACTCATCATCTCGACCCAAGAGGATAACTGTTGATTCGGGGGAAACTGAGTTTGGATATGAAGCCGAGTCTGGTTTACTCACCACTGATTTGAGAGTGCCAGAGAAAGAACTGCACATTTGGAACATCAGCATCGAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

758

Amino Acids

83.2

Weight (kDa)

5.44

Isoelectric Point (pI)

35.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 7 - 739 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012127)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55740
fragaria_vesca FvH4_3g41930 FvH4_3g41930 FvH4_3g41930
malus_domestica MD03G1040100.v1.1 MD11G1041500.v1.1
prunus_persica Prupe.6G032400_v2.0.a1 Prupe.6G032400_v2.0.a1
pyrus_communis pycom03g03080 pycom11g03510
rosa_chinensis RchiOBHm_Chr5g0075141
rosa_laevigata RLG00000036533
rosa_multiflora Rmu_ssc0000217.1_g000009
rosa_roxburghii Rroxscaffold_1G00006480
rosa_rugosa Rorug05G0436500
rosa_samantha Rh5AG493800 Rh5CG539100 Rh5DG527900
rosa_wichuraiana Rw5G045810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1358
AccB7I CCANNNNNTGG 1 cut(s) 1789
AccI GTMKAC 1 cut(s) 744
AccII CGCG 4 cut(s) 579, 1518, 1520, 2109
AciI CCGC 7 cut(s) 163, 344, 459, 966, 1345, 1520, 2109
AclI AACGTT 1 cut(s) 84
AclWI GGATC 8 cut(s) 354, 449, 514, 863, 876, 1382, 1612, 1655
AcsI RAATTY 2 cut(s) 830, 2009
AcuI CTGAAG 4 cut(s) 379, 639, 686, 1709
AfaI GTAC 2 cut(s) 625, 1593
AfiI CCNNNNNNNGG 6 cut(s) 104, 878, 1292, 1674, 1789, 1795
AflIII ACRYGT 1 cut(s) 600
AjnI CCWGG 6 cut(s) 976, 1033, 1555, 1627, 1740, 1788
AjuI GAANNNNNNNTTGG 4 cut(s) 113, 145, 2166, 2198
AleI CACNNNNGTG 1 cut(s) 647
AluBI AGCT 6 cut(s) 1157, 1352, 1514, 1588, 1748, 2105
AluI AGCT 6 cut(s) 1157, 1352, 1514, 1588, 1748, 2105
Alw26I GTCTC 2 cut(s) 272, 318
AlwI GGATC 8 cut(s) 354, 449, 514, 863, 876, 1382, 1612, 1655
AlwNI CAGNNNCTG 1 cut(s) 536
Ama87I CYCGRG 4 cut(s) 1106, 1205, 1668, 1953
AoxI GGCC 8 cut(s) 259, 661, 1014, 1093, 1213, 1379, 1617, 2023
ApeKI GCWGC 5 cut(s) 461, 785, 1456, 1511, 1561
ApoI RAATTY 2 cut(s) 830, 2009
ArsI GACNNNNNNTTYG 4 cut(s) 271, 296, 303, 328
Asp700I GAANNNNTTC 2 cut(s) 1115, 1300
AspLEI GCGC 1 cut(s) 1520
AspS9I GGNCC 1 cut(s) 1617
AsuC2I CCSGG 4 cut(s) 1797, 1810, 1954, 1955
AsuHPI GGTGA 2 cut(s) 335, 2203
AvaI CYCGRG 4 cut(s) 1106, 1205, 1668, 1953
BaeGI GKGCMC 1 cut(s) 1802
BamHI GGATCC 1 cut(s) 868
BanII GRGCYC 2 cut(s) 703, 1092
BbsI GAAGAC 6 cut(s) 163, 548, 1639, 1676, 1723, 1960
BbvI GCAGC 5 cut(s) 448, 797, 1468, 1523, 1573
BciT130I CCWGG 6 cut(s) 978, 1035, 1557, 1629, 1742, 1790
BciVI GTATCC 2 cut(s) 761, 1918
BclI TGATCA 1 cut(s) 148
BcnI CCSGG 4 cut(s) 1797, 1810, 1954, 1955
BcoDI GTCTC 2 cut(s) 272, 318
BfaI CTAG 4 cut(s) 443, 686, 1275, 1383
BfmI CTRYAG 2 cut(s) 677, 2019
BfuI GTATCC 2 cut(s) 761, 1918
BisI GCNGC 6 cut(s) 163, 462, 786, 1457, 1512, 1562
BlsI GCNGC 6 cut(s) 164, 463, 787, 1458, 1513, 1563
BmeT110I CYCGRG 4 cut(s) 1106, 1205, 1668, 1953
BmgT120I GGNCC 1 cut(s) 1617
BmiI GGNNCC 7 cut(s) 502, 700, 870, 1794, 1980, 2053, 2128
BpiI GAAGAC 6 cut(s) 163, 548, 1639, 1676, 1723, 1960
BpmI CTGGAG 2 cut(s) 1017, 1737
BpuEI CTTGAG 2 cut(s) 209, 1633
BpuMI CCSGG 4 cut(s) 1797, 1810, 1954, 1955
BsaAI YACGTR 1 cut(s) 627
BsaBI GATNNNNATC 1 cut(s) 434
BsaWI WCCGGW 1 cut(s) 216
BsaXI ACNNNNNCTCC 2 cut(s) 1161, 1191
Bsc4I CCNNNNNNNGG 6 cut(s) 104, 878, 1292, 1674, 1789, 1795
Bse1I ACTGG 2 cut(s) 958, 1720
Bse8I GATNNNNATC 1 cut(s) 434
BseBI CCWGG 6 cut(s) 978, 1035, 1557, 1629, 1742, 1790
BseJI GATNNNNATC 1 cut(s) 434
BseLI CCNNNNNNNGG 6 cut(s) 104, 878, 1292, 1674, 1789, 1795
BseMII CTCAG 4 cut(s) 60, 296, 1571, 2169
BseNI ACTGG 2 cut(s) 958, 1720
BseSI GKGCMC 1 cut(s) 1802
BseXI GCAGC 5 cut(s) 448, 797, 1468, 1523, 1573
BseYI CCCAGC 2 cut(s) 14, 1748
BsgI GTGCAG 2 cut(s) 1121, 2228
Bsh1236I CGCG 4 cut(s) 579, 1518, 1520, 2109
BshFI GGCC 8 cut(s) 261, 663, 1016, 1095, 1215, 1381, 1619, 2025
BsiHKCI CYCGRG 4 cut(s) 1106, 1205, 1668, 1953
BsiSI CCGG 7 cut(s) 98, 217, 504, 1460, 1796, 1809, 1954
BslFI GGGAC 1 cut(s) 404
BslI CCNNNNNNNGG 6 cut(s) 104, 878, 1292, 1674, 1789, 1795
BsmAI GTCTC 2 cut(s) 272, 318
BsmFI GGGAC 1 cut(s) 404
BsnI GGCC 8 cut(s) 261, 663, 1016, 1095, 1215, 1381, 1619, 2025
BsoBI CYCGRG 4 cut(s) 1106, 1205, 1668, 1953
Bsp1286I GDGCHC 3 cut(s) 703, 1092, 1802
Bsp143I GATC 9 cut(s) 148, 346, 454, 506, 868, 1387, 1573, 1604, 1660
Bsp19I CCATGG 1 cut(s) 1216
Bsp68I TCGCGA 1 cut(s) 579
BspACI CCGC 7 cut(s) 163, 344, 459, 966, 1345, 1520, 2109
BspANI GGCC 8 cut(s) 261, 663, 1016, 1095, 1215, 1381, 1619, 2025
BspCNI CTCAG 4 cut(s) 61, 295, 1572, 2170
BspFNI CGCG 4 cut(s) 579, 1518, 1520, 2109
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 7 cut(s) 502, 700, 870, 1794, 1980, 2053, 2128
BspMAI CTGCAG 1 cut(s) 681
BspPI GGATC 8 cut(s) 354, 449, 514, 863, 876, 1382, 1612, 1655
BsrI ACTGG 2 cut(s) 958, 1720
BssMI GATC 9 cut(s) 148, 346, 454, 506, 868, 1387, 1573, 1604, 1660
BssT1I CCWWGG 4 cut(s) 1216, 1441, 1845, 1920
Bst2UI CCWGG 6 cut(s) 978, 1035, 1557, 1629, 1742, 1790
Bst4CI ACNGT 9 cut(s) 7, 367, 547, 1337, 1435, 1975, 1995, 2092, 2161
BstAPI GCANNNNNTGC 3 cut(s) 676, 794, 1421
BstBAI YACGTR 1 cut(s) 627
BstC8I GCNNGC 5 cut(s) 945, 1213, 1484, 1516, 2107
BstDEI CTNAG 4 cut(s) 69, 282, 1580, 2178
BstDSI CCRYGG 2 cut(s) 546, 1216
BstFNI CGCG 4 cut(s) 579, 1518, 1520, 2109
BstHHI GCGC 1 cut(s) 1520
BstKTI GATC 9 cut(s) 151, 349, 457, 509, 871, 1390, 1576, 1607, 1663
BstMAI GTCTC 2 cut(s) 272, 318
BstMBI GATC 9 cut(s) 148, 346, 454, 506, 868, 1387, 1573, 1604, 1660
BstMWI GCNNNNNNNGC 9 cut(s) 258, 676, 782, 794, 1262, 1271, 1421, 1517, 1561
BstNI CCWGG 6 cut(s) 978, 1035, 1557, 1629, 1742, 1790
BstNSI RCATGY 2 cut(s) 604, 947
BstSFI CTRYAG 2 cut(s) 677, 2019
BstSLI GKGCMC 1 cut(s) 1802
BstUI CGCG 4 cut(s) 579, 1518, 1520, 2109
BstV1I GCAGC 5 cut(s) 448, 797, 1468, 1523, 1573
BstV2I GAAGAC 6 cut(s) 163, 548, 1639, 1676, 1723, 1960
BstX2I RGATCY 4 cut(s) 454, 868, 1387, 1604
BstXI CCANNNNNNTGG 1 cut(s) 1748
BstYI RGATCY 4 cut(s) 454, 868, 1387, 1604
BsuI GTATCC 2 cut(s) 761, 1918
BsuRI GGCC 8 cut(s) 261, 663, 1016, 1095, 1215, 1381, 1619, 2025
BtgI CCRYGG 2 cut(s) 546, 1216
BtgZI GCGATG 1 cut(s) 560
BtsIMutI CAGTG 3 cut(s) 1342, 1980, 2214
BtuMI TCGCGA 1 cut(s) 579
Cac8I GCNNGC 5 cut(s) 945, 1213, 1484, 1516, 2107
CaiI CAGNNNCTG 1 cut(s) 536
CciI TCATGA 1 cut(s) 76
CfoI GCGC 1 cut(s) 1520
Cfr13I GGNCC 1 cut(s) 1617
Cfr9I CCCGGG 1 cut(s) 1953
CseI GACGC 2 cut(s) 782, 1254
CsiI ACCWGGT 1 cut(s) 976
Csp6I GTAC 2 cut(s) 624, 1592
CviQI GTAC 2 cut(s) 624, 1592
DdeI CTNAG 4 cut(s) 69, 282, 1580, 2178
DpnI GATC 9 cut(s) 150, 348, 456, 508, 870, 1389, 1575, 1606, 1662
DpnII GATC 9 cut(s) 148, 346, 454, 506, 868, 1387, 1573, 1604, 1660
DraI TTTAAA 1 cut(s) 223
EciI GGCGGA 1 cut(s) 359
Eco130I CCWWGG 4 cut(s) 1216, 1441, 1845, 1920
Eco147I AGGCCT 2 cut(s) 663, 2025
Eco24I GRGCYC 2 cut(s) 703, 1092
Eco57I CTGAAG 4 cut(s) 379, 639, 686, 1709
Eco88I CYCGRG 4 cut(s) 1106, 1205, 1668, 1953
EcoRII CCWGG 6 cut(s) 976, 1033, 1555, 1627, 1740, 1788
EcoT14I CCWWGG 4 cut(s) 1216, 1441, 1845, 1920
EcoT38I GRGCYC 2 cut(s) 703, 1092
ErhI CCWWGG 4 cut(s) 1216, 1441, 1845, 1920
FaqI GGGAC 1 cut(s) 404
FauI CCCGC 1 cut(s) 466
FbaI TGATCA 1 cut(s) 148
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 6 cut(s) 163, 462, 786, 1457, 1512, 1562
FriOI GRGCYC 2 cut(s) 703, 1092
Fsp4HI GCNGC 6 cut(s) 163, 462, 786, 1457, 1512, 1562
FspBI CTAG 4 cut(s) 443, 686, 1275, 1383
GlaI GCGC 1 cut(s) 1519
GluI GCNGC 6 cut(s) 163, 462, 786, 1457, 1512, 1562
GsaI CCCAGC 2 cut(s) 18, 1752
GsuI CTGGAG 2 cut(s) 1017, 1737
HaeIII GGCC 8 cut(s) 261, 663, 1016, 1095, 1215, 1381, 1619, 2025
HapII CCGG 7 cut(s) 98, 217, 504, 1460, 1796, 1809, 1954
HgaI GACGC 2 cut(s) 782, 1254
HhaI GCGC 1 cut(s) 1520
Hin6I GCGC 1 cut(s) 1518
HinP1I GCGC 1 cut(s) 1518
HincII GTYRAC 2 cut(s) 745, 807
HindII GTYRAC 2 cut(s) 745, 807
HpaI GTTAAC 1 cut(s) 807
HpaII CCGG 7 cut(s) 98, 217, 504, 1460, 1796, 1809, 1954
HphI GGTGA 2 cut(s) 335, 2203
Hpy166II GTNNAC 5 cut(s) 624, 745, 807, 1102, 2207
Hpy8I GTNNAC 5 cut(s) 624, 745, 807, 1102, 2207
Hpy99I CGWCG 1 cut(s) 776
HpyAV CCTTC 4 cut(s) 320, 380, 653, 1218
HpyCH4III ACNGT 9 cut(s) 7, 367, 547, 1337, 1435, 1975, 1995, 2092, 2161
HpyCH4IV ACGT 2 cut(s) 84, 626
HpyF10VI GCNNNNNNNGC 9 cut(s) 258, 676, 782, 794, 1262, 1271, 1421, 1517, 1561
HpyF3I CTNAG 4 cut(s) 69, 282, 1580, 2178
HpySE526I ACGT 2 cut(s) 84, 626
HspAI GCGC 1 cut(s) 1518
Ksp22I TGATCA 1 cut(s) 148
KspAI GTTAAC 1 cut(s) 807
Kzo9I GATC 9 cut(s) 148, 346, 454, 506, 868, 1387, 1573, 1604, 1660
LmnI GCTCC 7 cut(s) 500, 698, 1508, 1745, 2020, 2051, 2126
Lsp1109I GCAGC 5 cut(s) 448, 797, 1468, 1523, 1573
MabI ACCWGGT 1 cut(s) 976
MaeI CTAG 4 cut(s) 443, 686, 1275, 1383
MaeII ACGT 2 cut(s) 84, 626
MaeIII GTNAC 6 cut(s) 91, 649, 895, 1313, 1547, 1711
MalI GATC 9 cut(s) 150, 348, 456, 508, 870, 1389, 1575, 1606, 1662
MboI GATC 9 cut(s) 148, 346, 454, 506, 868, 1387, 1573, 1604, 1660
MfeI CAATTG 1 cut(s) 1735
MflI RGATCY 4 cut(s) 454, 868, 1387, 1604
MhlI GDGCHC 3 cut(s) 703, 1092, 1802
MlyI GAGTC 6 cut(s) 274, 320, 1052, 1179, 1321, 2207
MmeI TCCRAC 4 cut(s) 745, 1504, 1744, 2022
MroXI GAANNNNTTC 2 cut(s) 1115, 1300
MseI TTAA 6 cut(s) 222, 423, 806, 906, 1946, 2272
MslI CAYNNNNRTG 3 cut(s) 321, 647, 1896
MspA1I CMGCKG 3 cut(s) 461, 1157, 1352
MspI CCGG 7 cut(s) 98, 217, 504, 1460, 1796, 1809, 1954
MunI CAATTG 1 cut(s) 1735
MvaI CCWGG 6 cut(s) 978, 1035, 1557, 1629, 1742, 1790
MvnI CGCG 4 cut(s) 579, 1518, 1520, 2109
MwoI GCNNNNNNNGC 9 cut(s) 258, 676, 782, 794, 1262, 1271, 1421, 1517, 1561
NciI CCSGG 4 cut(s) 1797, 1810, 1954, 1955
NcoI CCATGG 1 cut(s) 1216
NdeII GATC 9 cut(s) 148, 346, 454, 506, 868, 1387, 1573, 1604, 1660
NlaIV GGNNCC 7 cut(s) 502, 700, 870, 1794, 1980, 2053, 2128
NmeAIII GCCGAG 2 cut(s) 1071, 2221
NmuCI GTSAC 3 cut(s) 649, 1313, 1547
NruI TCGCGA 1 cut(s) 579
NspI RCATGY 2 cut(s) 604, 947
OliI CACNNNNGTG 1 cut(s) 647
PaeI GCATGC 1 cut(s) 947
PagI TCATGA 1 cut(s) 76
PceI AGGCCT 2 cut(s) 663, 2025
PciI ACATGT 1 cut(s) 600
PdmI GAANNNNTTC 2 cut(s) 1115, 1300
PfeI GAWTC 5 cut(s) 533, 752, 1004, 1699, 2165
PflMI CCANNNNNTGG 1 cut(s) 1789
PfoI TCCNGGA 2 cut(s) 1033, 1627
PkrI GCNGC 6 cut(s) 164, 463, 787, 1458, 1513, 1563
PleI GAGTC 6 cut(s) 274, 319, 1051, 1178, 1320, 2206
PpsI GAGTC 6 cut(s) 274, 319, 1051, 1178, 1320, 2206
Ppu21I YACGTR 1 cut(s) 627
PscI ACATGT 1 cut(s) 600
PsiI TTATAA 1 cut(s) 1358
Psp1406I AACGTT 1 cut(s) 84
Psp6I CCWGG 6 cut(s) 976, 1033, 1555, 1627, 1740, 1788
PspFI CCCAGC 2 cut(s) 14, 1748
PspGI CCWGG 6 cut(s) 976, 1033, 1555, 1627, 1740, 1788
PspN4I GGNNCC 7 cut(s) 502, 700, 870, 1794, 1980, 2053, 2128
PspPI GGNCC 1 cut(s) 1617
PstI CTGCAG 1 cut(s) 681
PstNI CAGNNNCTG 1 cut(s) 536
PsuI RGATCY 4 cut(s) 454, 868, 1387, 1604
PvuII CAGCTG 2 cut(s) 1157, 1352
RruI TCGCGA 1 cut(s) 579
RsaI GTAC 2 cut(s) 625, 1593
RsaNI GTAC 2 cut(s) 624, 1592
RseI CAYNNNNRTG 3 cut(s) 321, 647, 1896
SalI GTCGAC 1 cut(s) 743
SaqAI TTAA 6 cut(s) 222, 423, 806, 906, 1946, 2272
SatI GCNGC 6 cut(s) 163, 462, 786, 1457, 1512, 1562
Sau3AI GATC 9 cut(s) 148, 346, 454, 506, 868, 1387, 1573, 1604, 1660
Sau96I GGNCC 1 cut(s) 1617
SchI GAGTC 6 cut(s) 274, 320, 1052, 1179, 1321, 2207
SduI GDGCHC 3 cut(s) 703, 1092, 1802
SexAI ACCWGGT 1 cut(s) 976
SfcI CTRYAG 2 cut(s) 677, 2019
SmaI CCCGGG 1 cut(s) 1955
SmiMI CAYNNNNRTG 3 cut(s) 321, 647, 1896
SmlI CTYRAG 2 cut(s) 188, 1612
SmoI CTYRAG 2 cut(s) 188, 1612
SphI GCATGC 1 cut(s) 947
SseBI AGGCCT 2 cut(s) 663, 2025
SsiI CCGC 7 cut(s) 163, 344, 459, 966, 1345, 1520, 2109
SspMI CTAG 4 cut(s) 443, 686, 1275, 1383
StuI AGGCCT 2 cut(s) 663, 2025
StyI CCWWGG 4 cut(s) 1216, 1441, 1845, 1920
TaaI ACNGT 9 cut(s) 7, 367, 547, 1337, 1435, 1975, 1995, 2092, 2161
TaiI ACGT 2 cut(s) 87, 629
TaqI TCGA 6 cut(s) 384, 519, 744, 1572, 2143, 2265
TauI GCSGC 1 cut(s) 165
TfiI GAWTC 5 cut(s) 533, 752, 1004, 1699, 2165
Tru1I TTAA 6 cut(s) 222, 423, 806, 906, 1946, 2272
Tru9I TTAA 6 cut(s) 222, 423, 806, 906, 1946, 2272
TscAI CASTG 3 cut(s) 1342, 1980, 2221
TseFI GTSAC 3 cut(s) 649, 1313, 1547
TseI GCWGC 5 cut(s) 461, 785, 1456, 1511, 1561
Tsp45I GTSAC 3 cut(s) 649, 1313, 1547
TspGWI ACGGA 1 cut(s) 744
TspMI CCCGGG 1 cut(s) 1953
TspRI CASTG 3 cut(s) 1342, 1980, 2221
Van91I CCANNNNNTGG 1 cut(s) 1789
XapI RAATTY 2 cut(s) 830, 2009
XceI RCATGY 2 cut(s) 604, 947
XmaI CCCGGG 1 cut(s) 1953
XmiI GTMKAC 1 cut(s) 744
XmnI GAANNNNTTC 2 cut(s) 1115, 1300
XspI CTAG 4 cut(s) 443, 686, 1275, 1383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.