RLG00000036533

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012127
Physical Location & Seq
Reverse (-)
81612791 .. 81616430
3640 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036533

Sequence Viewer

Length: 2277 bp
ATGACGGTTGGGGCTGGAGTAACCGTTGAAGATGGAAGTTTGATGGTGTTGGGGAACAAGGTGTTGGGTGAAGTGCATGACAACGTTGTTGTAACTCCGGCCTCCGGCGGTGCATTGGCCAATGGGGCCTTCATCGGAGTTGAGTCTGATCAGAAGGGTAGCCGGAGAGTGTTTCCGATCGGTAAACTCGAGGGGTTGCGGTTTATGTGTGTATTTCGGTTTAAGATGTGGTGGATGACACAGAGGATGGGGAGTAGTGGTCAAGATGTGCCGTTTGAGACGCAGTTTTTGATTGTGGAGACCAAGGAAGGTGGTCATTTTGGTGAAGGAAGCAATATTGAAGGGGAGGAAGCTGCTGTGTACACCGTTTTCCTGCCGATTCTCGAGGGGGATTTCAGGGCTGTTCTTCAGGGGAATGAACTGAATGAGATTGAAATCTGCCTAGAAAGTGGAGATCCTGATGTTGATGGATTTGAGGGTAGTCATTTGGTTTTTGTTGGGGCTGGATCAGACCCATTTGAAGTCATCACAGATACAGTGAAGACTGTGGAGAAGCATTTACAGACATTCCATCATCGTGAGCGAAAGAAGATGCCAGACATGTTGAACTGGTTTGGCTGGTGTACATGGGATGCTTTCTACACTGATGTCACTTCAGAAGGTCTAAGGCAAGGGTTAGAGAGCTTCGAAAATGGTGGAGTGCCTCCGAAGTTTGTTATTATTGATGATGGATGGCAATCAGTTAGTATGGATCCCACTGGCGTTGGCTTCCTAGCTGATAACACAGCAAACTTTGCAAACAGATTGACAAATATTAAAGAGAACCACAAATTTCAAAAAGATGGTAAAGAGGGTCACAGAGTAGAGGATCCATCTTTGGGTCTTCGCCACATTGTATCTGAAATTAAAGAAAAACATGCTTTGAAGTATGCTTATGTGTGGCATGCTATAACTGGTTACTGGGGTGGTGTTAGACCTGGTGTTTCCGAAATGGAACACTACGACTCCAAGTTGGCATACCCTGTTTCATCTCCAGGGGTTGAGTCCAATGAGCCCTGTGATGCCTTCAACAGCATTGCCAAAAATGGCCTTGGCCTTGTAAATCCTGAGAAAGTTTTCCACTTCTATGATGAGCTCCACTCTTATCTCGCATCAGCAGGAATTGATGGGGTTAAAGTTGATGTTCAAAACATTCTCGAAACTTTAGGGGCAGGTCATGGTGGAAGGGTGAAACTTGCAAGAAAATACCATCAGGCATTAGAAGCGTCTATTGCTAGAAACTTTCCTGACAATGGTATCATTTCTTGTATGAGCCACAACACAGATGGTTTATACAGTGCGAAGAGGACAGCTGTTATAAGGGCATCAGATGATTTTTGGCCTAGAGATCCTGCATCACACACAATTCATATTGCATCAGTTGCTTATAACACTGTTTTTCTTGGAGAATTCATGCAGCCAGATTGGGATATGTTTCATAGCTTACACCCGATGGCTGAATATCATGGAGCAGCTCGTGCGGTGGGAGGATGTGCAATTTATGTTAGTGACAAGCCTGGGCAGCATGACTTCAATCTTTTAAGGAAGCTTGTACTTACTGATGGCTCCATCTTGAGGGCTAAACTACCAGGAAGACCAACAAGGGATTGCTTATTTTCTGATCCTGCTCGAGATGGGAAAAGTCTTCTGAAGATATGGAATCTGAATGATTTTACTGGAGTTGTGGGAGTCTTTAACTGCCAGGGAGCTGGGTGGTGTAAGGTTGGAAAGACGAACCTCATCCATGACGTAGAGCCGGGCACAGTTACAGGGGTTATTAGGGCTAAAGATGTTGATTATCTGCCCAAGATTGCACATGAGACATGGACTGGGGATGCTGTCATATATTCCCATCTTGGTGGAGAGGTGATATATCTTCACAAGGATGCATCTCGGCCAATTACTCTGAAATCCCGAGAGTACGAAGTTTTTACAGTGGTTCCTGCTAAGAAATTGTCTGATGGAGTAACATTTGCTCCCATCGGCCTAATCAAGATGTTCAATTCTGGGGGAGCCATAAAAGAATATGAATCTAAGAGCAGTACAACAGTTGACTTGAAAGTTCATGGGTCTGGTCTATTCGGAGCATATTCATCAGCTCGACCAAAGAGGATAACAGTTGATTCGGAGGATATGGAGTTTGGATATGAAGTTGAATCGGGTTTACTCACCATTGATTTGAGAGTTGCAGAGAAAGAATTGTACCTTTGGAACATCACCATTGAACTTTTGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

759

Amino Acids

83.5

Weight (kDa)

5.54

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 8 - 739 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012127)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55740
fragaria_vesca FvH4_3g41930 FvH4_3g41930 FvH4_3g41930
malus_domestica MD03G1040100.v1.1 MD11G1041500.v1.1
prunus_persica Prupe.6G032400_v2.0.a1 Prupe.6G032400_v2.0.a1
pyrus_communis pycom03g03080 pycom11g03510
rosa_chinensis RchiOBHm_Chr5g0075141
rosa_laevigata RLG00000036533
rosa_multiflora Rmu_ssc0000217.1_g000009
rosa_roxburghii Rroxscaffold_1G00006480
rosa_rugosa Rorug05G0436500
rosa_samantha Rh5AG493800 Rh5CG539100 Rh5DG527900
rosa_wichuraiana Rw5G045810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1358, 1428
Acc36I ACCTGC 1 cut(s) 1202
AciI CCGC 3 cut(s) 108, 199, 1520
AclI AACGTT 1 cut(s) 84
AclWI GGATC 8 cut(s) 449, 514, 746, 759, 863, 876, 1382, 1655
AcoI YGGCCR 2 cut(s) 117, 1934
AcsI RAATTY 2 cut(s) 830, 1448
AcuI CTGAAG 3 cut(s) 392, 639, 1709
AfaI GTAC 6 cut(s) 362, 625, 1593, 1961, 2083, 2243
AfiI CCNNNNNNNGG 4 cut(s) 104, 878, 1292, 1614
AflIII ACRYGT 1 cut(s) 600
AjnI CCWGG 5 cut(s) 976, 1033, 1555, 1627, 1740
AjuI GAANNNNNNNTTGG 4 cut(s) 47, 79, 113, 145
Alw21I GWGCWC 1 cut(s) 1137
Alw26I GTCTC 3 cut(s) 272, 293, 1853
AlwI GGATC 8 cut(s) 449, 514, 746, 759, 863, 876, 1382, 1655
Ama87I CYCGRG 4 cut(s) 188, 383, 1668, 1953
AoxI GGCC 8 cut(s) 99, 117, 126, 1087, 1093, 1379, 1934, 2023
ApeKI GCWGC 4 cut(s) 353, 1456, 1511, 1561
ApoI RAATTY 2 cut(s) 830, 1448
ArsI GACNNNNNNTTYG 2 cut(s) 271, 303
Asp700I GAANNNNTTC 1 cut(s) 1115
AspS9I GGNCC 1 cut(s) 126
AsuC2I CCSGG 1 cut(s) 1797
AsuHPI GGTGA 6 cut(s) 80, 335, 1240, 1918, 2200, 2248
AsuII TTCGAA 1 cut(s) 687
AvaI CYCGRG 4 cut(s) 188, 383, 1668, 1953
BaeGI GKGCMC 1 cut(s) 1802
BaeI ACNNNNGTAYC 2 cut(s) 1279, 1312
BalI TGGCCA 1 cut(s) 119
BamHI GGATCC 2 cut(s) 751, 868
BanII GRGCYC 2 cut(s) 1056, 1137
BauI CACGAG 1 cut(s) 1515
BbsI GAAGAC 4 cut(s) 548, 875, 1639, 1676
Bbv12I GWGCWC 1 cut(s) 1137
BbvI GCAGC 4 cut(s) 340, 1468, 1523, 1573
BceAI ACGGC 1 cut(s) 256
BciT130I CCWGG 5 cut(s) 978, 1035, 1557, 1629, 1742
BclI TGATCA 1 cut(s) 148
BcnI CCSGG 1 cut(s) 1797
BcoDI GTCTC 3 cut(s) 272, 293, 1853
BfaI CTAG 4 cut(s) 443, 773, 1275, 1383
BfuAI ACCTGC 1 cut(s) 1202
BglI GCCNNNNNGGC 1 cut(s) 125
BisI GCNGC 4 cut(s) 354, 1457, 1512, 1562
BlsI GCNGC 4 cut(s) 355, 1458, 1513, 1563
Bme1390I CCNGG 6 cut(s) 978, 1035, 1557, 1629, 1742, 1797
BmeT110I CYCGRG 4 cut(s) 188, 383, 1668, 1953
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 6 cut(s) 127, 753, 870, 1606, 1980, 2053
BmrFI CCNGG 6 cut(s) 978, 1035, 1557, 1629, 1742, 1797
BmrI ACTGGG 2 cut(s) 970, 1878
BmuI ACTGGG 2 cut(s) 970, 1878
BpiI GAAGAC 4 cut(s) 548, 875, 1639, 1676
BplI GAGNNNNNCTC 2 cut(s) 1124, 1156
BpmI CTGGAG 3 cut(s) 36, 1017, 1737
Bpu14I TTCGAA 1 cut(s) 687
BpuEI CTTGAG 1 cut(s) 1633
BpuMI CCSGG 1 cut(s) 1797
BsaBI GATNNNNATC 2 cut(s) 434, 736
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 5 cut(s) 303, 1034, 1090, 1556, 1741
BsaXI ACNNNNNCTCC 8 cut(s) 9, 39, 244, 274, 989, 1019, 1999, 2029
Bsc4I CCNNNNNNNGG 4 cut(s) 104, 878, 1292, 1614
Bse1I ACTGG 6 cut(s) 614, 763, 958, 965, 1720, 1873
Bse3DI GCAATG 1 cut(s) 1074
Bse8I GATNNNNATC 2 cut(s) 434, 736
BseBI CCWGG 5 cut(s) 978, 1035, 1557, 1629, 1742
BseDI CCNNGG 5 cut(s) 303, 1034, 1090, 1556, 1741
BseGI GGATG 8 cut(s) 240, 252, 637, 737, 1535, 1779, 1879, 1930
BseJI GATNNNNATC 2 cut(s) 434, 736
BseLI CCNNNNNNNGG 4 cut(s) 104, 878, 1292, 1614
BseMI GCAATG 1 cut(s) 1074
BseMII CTCAG 1 cut(s) 1098
BseNI ACTGG 6 cut(s) 614, 763, 958, 965, 1720, 1873
BseSI GKGCMC 1 cut(s) 1802
BseXI GCAGC 4 cut(s) 340, 1468, 1523, 1573
BseYI CCCAGC 1 cut(s) 1748
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 8 cut(s) 101, 119, 128, 1089, 1095, 1381, 1936, 2025
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 1137
BsiHKCI CYCGRG 4 cut(s) 188, 383, 1668, 1953
BsiSI CCGG 4 cut(s) 98, 105, 163, 1796
BslI CCNNNNNNNGG 4 cut(s) 104, 878, 1292, 1614
BsmAI GTCTC 3 cut(s) 272, 293, 1853
BsmBI CGTCTC 1 cut(s) 272
BsnI GGCC 8 cut(s) 101, 119, 128, 1089, 1095, 1381, 1936, 2025
Bso31I GGTCTC 1 cut(s) 293
BsoBI CYCGRG 4 cut(s) 188, 383, 1668, 1953
Bsp119I TTCGAA 1 cut(s) 687
Bsp1286I GDGCHC 3 cut(s) 1056, 1137, 1802
Bsp1407I TGTACA 2 cut(s) 360, 623
Bsp143I GATC 8 cut(s) 148, 177, 454, 506, 751, 868, 1387, 1660
BspACI CCGC 3 cut(s) 108, 199, 1520
BspANI GGCC 8 cut(s) 101, 119, 128, 1089, 1095, 1381, 1936, 2025
BspCNI CTCAG 1 cut(s) 1099
BspLI GGNNCC 6 cut(s) 127, 753, 870, 1606, 1980, 2053
BspMI ACCTGC 1 cut(s) 1202
BspPI GGATC 8 cut(s) 449, 514, 746, 759, 863, 876, 1382, 1655
BspT104I TTCGAA 1 cut(s) 687
BspTNI GGTCTC 1 cut(s) 293
BsrDI GCAATG 1 cut(s) 1074
BsrGI TGTACA 2 cut(s) 360, 623
BsrI ACTGG 6 cut(s) 614, 763, 958, 965, 1720, 1873
BssECI CCNNGG 5 cut(s) 303, 1034, 1090, 1556, 1741
BssMI GATC 8 cut(s) 148, 177, 454, 506, 751, 868, 1387, 1660
BssSI CACGAG 1 cut(s) 1515
BssT1I CCWWGG 2 cut(s) 303, 1090
Bst2BI CACGAG 1 cut(s) 1515
Bst2UI CCWGG 5 cut(s) 978, 1035, 1557, 1629, 1742
Bst6I CTCTTC 1 cut(s) 1337
BstAPI GCANNNNNTGC 3 cut(s) 794, 1421, 1517
BstAUI TGTACA 2 cut(s) 360, 623
BstBI TTCGAA 1 cut(s) 687
BstC8I GCNNGC 1 cut(s) 945
BstDEI CTNAG 4 cut(s) 665, 1107, 1986, 2073
BstF5I GGATG 8 cut(s) 240, 252, 637, 737, 1535, 1779, 1879, 1930
BstKTI GATC 8 cut(s) 151, 180, 457, 509, 754, 871, 1390, 1663
BstMAI GTCTC 3 cut(s) 272, 293, 1853
BstMBI GATC 8 cut(s) 148, 177, 454, 506, 751, 868, 1387, 1660
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 7 cut(s) 125, 794, 1262, 1271, 1421, 1517, 1561
BstNI CCWGG 5 cut(s) 978, 1035, 1557, 1629, 1742
BstNSI RCATGY 3 cut(s) 604, 920, 947
BstSCI CCNGG 6 cut(s) 976, 1033, 1555, 1627, 1740, 1795
BstSLI GKGCMC 1 cut(s) 1802
BstV1I GCAGC 4 cut(s) 340, 1468, 1523, 1573
BstV2I GAAGAC 4 cut(s) 548, 875, 1639, 1676
BstX2I RGATCY 4 cut(s) 454, 751, 868, 1387
BstXI CCANNNNNNTGG 2 cut(s) 1748, 1898
BstYI RGATCY 4 cut(s) 454, 751, 868, 1387
BsuRI GGCC 8 cut(s) 101, 119, 128, 1089, 1095, 1381, 1936, 2025
BtsCI GGATG 8 cut(s) 240, 252, 637, 737, 1535, 1779, 1879, 1930
BtsIMutI CAGTG 6 cut(s) 543, 642, 756, 1342, 1431, 1980
BveI ACCTGC 1 cut(s) 1202
Cac8I GCNNGC 1 cut(s) 945
Cfr13I GGNCC 1 cut(s) 126
CseI GACGC 2 cut(s) 289, 1254
CsiI ACCWGGT 1 cut(s) 976
Csp6I GTAC 6 cut(s) 361, 624, 1592, 1960, 2082, 2242
CspCI CAANNNNNGTGG 4 cut(s) 292, 327, 745, 780
CviQI GTAC 6 cut(s) 361, 624, 1592, 1960, 2082, 2242
DdeI CTNAG 4 cut(s) 665, 1107, 1986, 2073
DpnI GATC 8 cut(s) 150, 179, 456, 508, 753, 870, 1389, 1662
DpnII GATC 8 cut(s) 148, 177, 454, 506, 751, 868, 1387, 1660
EaeI YGGCCR 2 cut(s) 117, 1934
Eam1104I CTCTTC 1 cut(s) 1337
EarI CTCTTC 1 cut(s) 1337
Ecl136II GAGCTC 1 cut(s) 1135
Eco130I CCWWGG 2 cut(s) 303, 1090
Eco24I GRGCYC 2 cut(s) 1056, 1137
Eco31I GGTCTC 1 cut(s) 293
Eco53kI GAGCTC 1 cut(s) 1135
Eco57I CTGAAG 3 cut(s) 392, 639, 1709
Eco88I CYCGRG 4 cut(s) 188, 383, 1668, 1953
EcoICRI GAGCTC 1 cut(s) 1135
EcoO109I RGGNCCY 1 cut(s) 126
EcoRI GAATTC 1 cut(s) 1448
EcoRII CCWGG 5 cut(s) 976, 1033, 1555, 1627, 1740
EcoT14I CCWWGG 2 cut(s) 303, 1090
EcoT22I ATGCAT 1 cut(s) 1930
EcoT38I GRGCYC 2 cut(s) 1056, 1137
ErhI CCWWGG 2 cut(s) 303, 1090
Esp3I CGTCTC 1 cut(s) 272
FbaI TGATCA 1 cut(s) 148
Fnu4HI GCNGC 4 cut(s) 354, 1457, 1512, 1562
FokI GGATG 8 cut(s) 247, 259, 644, 744, 1542, 1766, 1886, 1937
FriOI GRGCYC 2 cut(s) 1056, 1137
Fsp4HI GCNGC 4 cut(s) 354, 1457, 1512, 1562
FspBI CTAG 4 cut(s) 443, 773, 1275, 1383
GluI GCNGC 4 cut(s) 354, 1457, 1512, 1562
GsaI CCCAGC 1 cut(s) 1752
GsuI CTGGAG 3 cut(s) 36, 1017, 1737
HaeIII GGCC 8 cut(s) 101, 119, 128, 1089, 1095, 1381, 1936, 2025
HapII CCGG 4 cut(s) 98, 105, 163, 1796
HgaI GACGC 2 cut(s) 289, 1254
HincII GTYRAC 1 cut(s) 2092
HindII GTYRAC 1 cut(s) 2092
HindIII AAGCTT 1 cut(s) 1586
HinfI GANTC 9 cut(s) 143, 379, 1004, 1043, 1699, 1728, 2069, 2162, 2195
HpaII CCGG 4 cut(s) 98, 105, 163, 1796
HphI GGTGA 6 cut(s) 80, 335, 1240, 1918, 2200, 2248
Hpy166II GTNNAC 6 cut(s) 185, 361, 363, 624, 2092, 2204
Hpy8I GTNNAC 6 cut(s) 185, 361, 363, 624, 2092, 2204
HpyAV CCTTC 8 cut(s) 139, 148, 302, 320, 335, 653, 1075, 1218
HpyCH4IV ACGT 2 cut(s) 84, 1788
HpyF10VI GCNNNNNNNGC 7 cut(s) 125, 794, 1262, 1271, 1421, 1517, 1561
HpyF3I CTNAG 4 cut(s) 665, 1107, 1986, 2073
HpySE526I ACGT 2 cut(s) 84, 1788
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 8 cut(s) 148, 177, 454, 506, 751, 868, 1387, 1660
LmnI GCTCC 7 cut(s) 1140, 1508, 1610, 1745, 2020, 2051, 2123
Lsp1109I GCAGC 4 cut(s) 340, 1468, 1523, 1573
MabI ACCWGGT 1 cut(s) 976
MaeI CTAG 4 cut(s) 443, 773, 1275, 1383
MaeII ACGT 2 cut(s) 84, 1788
MaeIII GTNAC 8 cut(s) 19, 91, 649, 854, 956, 1547, 1804, 2005
MalI GATC 8 cut(s) 150, 179, 456, 508, 753, 870, 1389, 1662
MboI GATC 8 cut(s) 148, 177, 454, 506, 751, 868, 1387, 1660
MflI RGATCY 4 cut(s) 454, 751, 868, 1387
MhlI GDGCHC 3 cut(s) 1056, 1137, 1802
MlsI TGGCCA 1 cut(s) 119
MluNI TGGCCA 1 cut(s) 119
MlyI GAGTC 4 cut(s) 152, 998, 1052, 1737
MmeI TCCRAC 1 cut(s) 1744
Mox20I TGGCCA 1 cut(s) 119
Mph1103I ATGCAT 1 cut(s) 1930
MroXI GAANNNNTTC 1 cut(s) 1115
MscI TGGCCA 1 cut(s) 119
MseI TTAA 6 cut(s) 222, 816, 906, 1173, 1580, 1734
MslI CAYNNNNRTG 5 cut(s) 321, 576, 1125, 1896, 1923
Msp20I TGGCCA 1 cut(s) 119
MspA1I CMGCKG 1 cut(s) 1352
MspI CCGG 4 cut(s) 98, 105, 163, 1796
MspR9I CCNGG 6 cut(s) 978, 1035, 1557, 1629, 1742, 1797
MvaI CCWGG 5 cut(s) 978, 1035, 1557, 1629, 1742
MwoI GCNNNNNNNGC 7 cut(s) 125, 794, 1262, 1271, 1421, 1517, 1561
NciI CCSGG 1 cut(s) 1797
NdeII GATC 8 cut(s) 148, 177, 454, 506, 751, 868, 1387, 1660
NlaIV GGNNCC 6 cut(s) 127, 753, 870, 1606, 1980, 2053
NmeAIII GCCGAG 1 cut(s) 1912
NmuCI GTSAC 3 cut(s) 649, 854, 1547
NsiI ATGCAT 1 cut(s) 1930
NspI RCATGY 3 cut(s) 604, 920, 947
NspV TTCGAA 1 cut(s) 687
PaeI GCATGC 1 cut(s) 947
PaeR7I CTCGAG 3 cut(s) 188, 383, 1668
PciI ACATGT 1 cut(s) 600
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 1115
PfeI GAWTC 5 cut(s) 379, 1699, 2069, 2162, 2195
PkrI GCNGC 4 cut(s) 355, 1458, 1513, 1563
Ple19I CGATCG 1 cut(s) 180
PleI GAGTC 4 cut(s) 151, 998, 1051, 1736
PpsI GAGTC 4 cut(s) 151, 998, 1051, 1736
PscI ACATGT 1 cut(s) 600
PsiI TTATAA 2 cut(s) 1358, 1428
Psp124BI GAGCTC 1 cut(s) 1137
Psp1406I AACGTT 1 cut(s) 84
Psp6I CCWGG 5 cut(s) 976, 1033, 1555, 1627, 1740
PspFI CCCAGC 1 cut(s) 1748
PspGI CCWGG 5 cut(s) 976, 1033, 1555, 1627, 1740
PspN4I GGNNCC 6 cut(s) 127, 753, 870, 1606, 1980, 2053
PspPI GGNCC 1 cut(s) 126
PspXI VCTCGAGB 1 cut(s) 188
PsuI RGATCY 4 cut(s) 454, 751, 868, 1387
PvuI CGATCG 1 cut(s) 180
PvuII CAGCTG 1 cut(s) 1352
RsaI GTAC 6 cut(s) 362, 625, 1593, 1961, 2083, 2243
RsaNI GTAC 6 cut(s) 361, 624, 1592, 1960, 2082, 2242
RseI CAYNNNNRTG 5 cut(s) 321, 576, 1125, 1896, 1923
SacI GAGCTC 1 cut(s) 1137
SaqAI TTAA 6 cut(s) 222, 816, 906, 1173, 1580, 1734
SatI GCNGC 4 cut(s) 354, 1457, 1512, 1562
Sau3AI GATC 8 cut(s) 148, 177, 454, 506, 751, 868, 1387, 1660
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 4 cut(s) 152, 998, 1052, 1737
ScrFI CCNGG 6 cut(s) 978, 1035, 1557, 1629, 1742, 1797
SduI GDGCHC 3 cut(s) 1056, 1137, 1802
SexAI ACCWGGT 1 cut(s) 976
SfiI GGCCNNNNNGGCC 1 cut(s) 125
Sfr274I CTCGAG 3 cut(s) 188, 383, 1668
SfuI TTCGAA 1 cut(s) 687
SlaI CTCGAG 3 cut(s) 188, 383, 1668
SmiMI CAYNNNNRTG 5 cut(s) 321, 576, 1125, 1896, 1923
SmlI CTYRAG 4 cut(s) 188, 383, 1612, 1668
SmoI CTYRAG 4 cut(s) 188, 383, 1612, 1668
SphI GCATGC 1 cut(s) 947
SsiI CCGC 3 cut(s) 108, 199, 1520
SspI AATATT 2 cut(s) 337, 814
SspMI CTAG 4 cut(s) 443, 773, 1275, 1383
SstI GAGCTC 1 cut(s) 1137
StyD4I CCNGG 6 cut(s) 976, 1033, 1555, 1627, 1740, 1795
StyI CCWWGG 2 cut(s) 303, 1090
TaiI ACGT 2 cut(s) 87, 1791
TaqI TCGA 6 cut(s) 189, 384, 687, 1197, 1669, 2140
TatI WGTACW 4 cut(s) 360, 623, 1591, 2081
TfiI GAWTC 5 cut(s) 379, 1699, 2069, 2162, 2195
Tru1I TTAA 6 cut(s) 222, 816, 906, 1173, 1580, 1734
Tru9I TTAA 6 cut(s) 222, 816, 906, 1173, 1580, 1734
TscAI CASTG 6 cut(s) 543, 649, 763, 1342, 1438, 1980
TseFI GTSAC 3 cut(s) 649, 854, 1547
TseI GCWGC 4 cut(s) 353, 1456, 1511, 1561
Tsp45I GTSAC 3 cut(s) 649, 854, 1547
TspRI CASTG 6 cut(s) 543, 649, 763, 1342, 1438, 1980
XapI RAATTY 2 cut(s) 830, 1448
XceI RCATGY 3 cut(s) 604, 920, 947
XcmI CCANNNNNNNNNTGG 1 cut(s) 1322
XhoI CTCGAG 3 cut(s) 188, 383, 1668
XmnI GAANNNNTTC 1 cut(s) 1115
XspI CTAG 4 cut(s) 443, 773, 1275, 1383
Zsp2I ATGCAT 1 cut(s) 1930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.