Rroxscaffold_1G00006480

galactinol--sucrose galactosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012127
Physical Location & Seq
Forward (+)
8530263 .. 8534179
3917 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00006480.1

Sequence Viewer

Length: 2271 bp
ATGACGGTTGGGGCAGGAATAACCGTTGAAGATGGAAGTTTGATGGTGTTGGGGAACAAGGTGTTGGGTGAAGTGCATGACAACGTTGTTGTAACCCCGGCCTCCGGCGGTGCATTGGCCAATGGGGCCTTCATCGGAGTTGAGTCTGATCAGAAGGGTAGCCGGAGAGTGTTTCCGATCGGTAAACTCGAGGGGTTGCGGTTTATGTGTGTATTTCGGTTTAAGATGTGGTGGATGACACAGAGGATGGGGACTAGTGGTCAAGATGTGCCGTTTGAGACGCAGTTTTTGATTGTGGAGACCAAGGAAGGTGGTCATTTTGGTGAAGGAAGCAATACTGAAGGGGAGGAATCTGCTGTGTATACCGTTTTCTTGCCGATTCTCGAGGGGGATTTCAGGGCTGTTCTTCAGGGGAATGAACTGAATGAGATTGAAATCTGCCTAGAAAGTGGAGATCCTGATGTTGATGGATTTGAGGGTAGACATTTGGTTTTTGTTGGGGCTGGATCAGACCCATTTGAAGTCATCACAGATACTGTGAAGACTGTGGAGAAGCATTTACAGACATTCCATCATCGTGAGCGAAAGAAGATGCCAGACATGTTGAACTGGTTTGGTTGGTGTACATGGGATGCTTTCTACACTGATGTCACTTCAGACGGTCTAAAGCAAGGGTTACAGAGCTTCGAAAATGGTGGAGTGCCTCCAAAGTTTGTTATTATTGATGATGGATGGCAATCAGTTAGTATGGATTCCACTGGTGTTGGCTTCCTAGCTGATAACACAGCAAACTTTGCAAACAGATTGACAAATATTAAAGAGAACCACAAATTTCAAAAAGATGGTAAAGAGGGTCACAGAGTAGAGGATCCATCTTTGGGCCTTCGCCACATTGTATCTGAAATTAAAGAAAAACATGCTTTGAAGTATGCTTATGTGTGGCATGCTATAACTGGTTACTGGGGTGGTGTTAGACCTGGTGTATCTGAAATGGAACACTACGACTCCAAGTTGGCCTACCCTGTTTCATCTCCAGGGGTTGAGTCCAATGAGTCCTGTGATGCCTTCAACAGCATTGCCAAAAATGGCCTTGGCCTTGTGAATCCTGAGAAAGTTTTCCATTTCTATGATGAGCTCCACTCTTATCTCGCATCAGCAGGAATTGATGGGGTTAAAGTTGATGTTCAAAACATTCTCGAAACTTTAGGGGCAGGTCATGGTGGAAGGGTGAAACTTGCAAGAAAATACCATCAGGCATTAGAAGCCTCTATTGCTAGAAACTTTCCTGACAATGGTATTATTTCTTGTATGAGCCACAACACGGATGGTTTATACAGTGCAAAGAGGACAGCTGTTATAAGGGCATCAGATGATTTTTGGCCTAGAGATCCAGCATCACACACAATTCATATTGCATCAGTTGCTTATAACACTGTTTTTCTTGGAGAATTCATGCAGCCAGATTGGGATATGTTTCATAGCTTACACCCGATGGCTGAATATCATGGAGCAGCTCGTGCGGTGGGAGGATGTGCAATTTATGTTAGTGACAAGCCTGGGCAGCATGACTTCAATCTTTTAAGGAAGCTTGTACTTACTGATGGCTCCATCTTGAGGGCTAAACTACCAGGAAGACCAACAAGGGATTGCTTATTTTCTGATCCTGCGCGGGATGGGAAAAGTCTTCTGAAGATATGGAATCTGAATGATTTTACTGGAGTTGTGGGAGTCTTTAACTGCCAGGGAGCCGGGTGGTGTAAGGTTGGAAAGACGAACCTCATCCATGACGTAGAGCCGGGCACAGTTACAGGGGTTATTAGGGCTAAAGATGTTGATTATCTGCCCAAGGTTGCACATGAGACATGGACTGGGGATACTGTCATATATTCCCATCTTGGTGGAGAGGTGGTATATCTTCCCAAGGAAGCATCTTGGCCAATTACACTGAAATCCCGAGAGTACGAAGTTTTTACAGTGGTTCCTGCTAAGAAATTGTCTGATGGTGTAACATTTGCTCCCATCGGCCTAATCAAGATGTTCAATTCTGGGGGAGCCATAAAAGAATATGAATCTAAGAGCAGTACAACAGTTGACATGAAAGTTCATGGGTCTGGTCTATTCGCAGCATATTCATCAGCTCGACCAAAGAGGATAACAGTTGATTCGGAGGATATGGAGTTTGGATATGAAGTTGCATCGGGTTTACTCACCATTGATTTGAGAATTGCAGAGAAAGAATTGTACCTTTGGAACATCACCATTGAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 map00052
Pfam Domains
Protein Families
Raffinose_syn

Protein Analysis

756

Amino Acids

83.31

Weight (kDa)

5.58

Isoelectric Point (pI)

31.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 7 - 739 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012127)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55740
fragaria_vesca FvH4_3g41930 FvH4_3g41930 FvH4_3g41930
malus_domestica MD03G1040100.v1.1 MD11G1041500.v1.1
prunus_persica Prupe.6G032400_v2.0.a1 Prupe.6G032400_v2.0.a1
pyrus_communis pycom03g03080 pycom11g03510
rosa_chinensis RchiOBHm_Chr5g0075141
rosa_laevigata RLG00000036533
rosa_multiflora Rmu_ssc0000217.1_g000009
rosa_roxburghii Rroxscaffold_1G00006480
rosa_rugosa Rorug05G0436500
rosa_samantha Rh5AG493800 Rh5CG539100 Rh5DG527900
rosa_wichuraiana Rw5G045810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1358, 1428
Acc36I ACCTGC 1 cut(s) 1202
AccI GTMKAC 2 cut(s) 362, 481
AccII CGCG 1 cut(s) 1669
AciI CCGC 4 cut(s) 108, 199, 1520, 1669
AclI AACGTT 1 cut(s) 84
AclWI GGATC 6 cut(s) 449, 514, 863, 876, 1382, 1655
AcoI YGGCCR 2 cut(s) 117, 1934
AcsI RAATTY 2 cut(s) 830, 1448
AcuI CTGAAG 4 cut(s) 360, 392, 639, 1709
AfaI GTAC 5 cut(s) 625, 1593, 1961, 2083, 2243
AfiI CCNNNNNNNGG 5 cut(s) 104, 878, 1292, 1321, 1614
AflIII ACRYGT 1 cut(s) 600
AhdI GACNNNNNGTC 1 cut(s) 258
AhlI ACTAGT 1 cut(s) 254
AjnI CCWGG 5 cut(s) 976, 1033, 1555, 1627, 1740
AjuI GAANNNNNNNTTGG 4 cut(s) 47, 79, 113, 145
AluBI AGCT 8 cut(s) 684, 776, 1135, 1352, 1482, 1514, 1588, 2138
AluI AGCT 8 cut(s) 684, 776, 1135, 1352, 1482, 1514, 1588, 2138
Alw21I GWGCWC 1 cut(s) 1137
Alw26I GTCTC 3 cut(s) 272, 293, 1853
AlwI GGATC 6 cut(s) 449, 514, 863, 876, 1382, 1655
AlwNI CAGNNNCTG 1 cut(s) 536
Ama87I CYCGRG 3 cut(s) 188, 383, 1953
ApeKI GCWGC 4 cut(s) 1456, 1511, 1561, 2123
ApoI RAATTY 2 cut(s) 830, 1448
ArsI GACNNNNNNTTYG 2 cut(s) 271, 303
Asp700I GAANNNNTTC 1 cut(s) 1115
AspLEI GCGC 1 cut(s) 1669
AspS9I GGNCC 2 cut(s) 126, 880
AsuC2I CCSGG 3 cut(s) 98, 1750, 1797
AsuHPI GGTGA 5 cut(s) 80, 335, 1240, 2200, 2248
AsuII TTCGAA 1 cut(s) 687
AvaI CYCGRG 3 cut(s) 188, 383, 1953
BaeGI GKGCMC 1 cut(s) 1802
BalI TGGCCA 2 cut(s) 119, 1936
BamHI GGATCC 1 cut(s) 868
BanII GRGCYC 1 cut(s) 1137
BauI CACGAG 1 cut(s) 1515
BbsI GAAGAC 3 cut(s) 548, 1639, 1676
Bbv12I GWGCWC 1 cut(s) 1137
BbvI GCAGC 4 cut(s) 1468, 1523, 1573, 2135
BceAI ACGGC 1 cut(s) 256
BciT130I CCWGG 5 cut(s) 978, 1035, 1557, 1629, 1742
BciVI GTATCC 1 cut(s) 1867
BclI TGATCA 1 cut(s) 148
BcnI CCSGG 3 cut(s) 98, 1750, 1797
BcoDI GTCTC 3 cut(s) 272, 293, 1853
BcuI ACTAGT 1 cut(s) 254
BfaI CTAG 5 cut(s) 255, 443, 773, 1275, 1383
BfuAI ACCTGC 1 cut(s) 1202
BfuI GTATCC 1 cut(s) 1867
BglI GCCNNNNNGGC 1 cut(s) 125
BisI GCNGC 4 cut(s) 1457, 1512, 1562, 2124
BlsI GCNGC 4 cut(s) 1458, 1513, 1563, 2125
Bme1390I CCNGG 8 cut(s) 98, 978, 1035, 1557, 1629, 1742, 1750, 1797
BmeRI GACNNNNNGTC 1 cut(s) 258
BmeT110I CYCGRG 3 cut(s) 188, 383, 1953
BmgT120I GGNCC 2 cut(s) 126, 880
BmiI GGNNCC 6 cut(s) 127, 870, 1606, 1747, 1980, 2053
BmrFI CCNGG 8 cut(s) 98, 978, 1035, 1557, 1629, 1742, 1750, 1797
BmrI ACTGGG 2 cut(s) 970, 1878
BmsI GCATC 9 cut(s) 582, 622, 1051, 1160, 1373, 1403, 1424, 1937, 2204
BmuI ACTGGG 2 cut(s) 970, 1878
BpiI GAAGAC 3 cut(s) 548, 1639, 1676
BplI GAGNNNNNCTC 2 cut(s) 1124, 1156
BpmI CTGGAG 2 cut(s) 1017, 1737
Bpu14I TTCGAA 1 cut(s) 687
BpuEI CTTGAG 1 cut(s) 1633
BpuMI CCSGG 3 cut(s) 98, 1750, 1797
BsaBI GATNNNNATC 2 cut(s) 434, 736
BsaI GGTCTC 1 cut(s) 293
BsaJI CCNNGG 8 cut(s) 96, 303, 1034, 1090, 1556, 1741, 1845, 1920
BsaXI ACNNNNNCTCC 6 cut(s) 989, 1019, 1893, 1923, 1999, 2029
Bsc4I CCNNNNNNNGG 5 cut(s) 104, 878, 1292, 1321, 1614
Bse1I ACTGG 6 cut(s) 614, 763, 958, 965, 1720, 1873
Bse3DI GCAATG 1 cut(s) 1074
Bse8I GATNNNNATC 2 cut(s) 434, 736
BseBI CCWGG 5 cut(s) 978, 1035, 1557, 1629, 1742
BseDI CCNNGG 8 cut(s) 96, 303, 1034, 1090, 1556, 1741, 1845, 1920
BseGI GGATG 8 cut(s) 240, 252, 637, 737, 1330, 1535, 1678, 1779
BseJI GATNNNNATC 2 cut(s) 434, 736
BseLI CCNNNNNNNGG 5 cut(s) 104, 878, 1292, 1321, 1614
BseMI GCAATG 1 cut(s) 1074
BseMII CTCAG 1 cut(s) 1098
BseNI ACTGG 6 cut(s) 614, 763, 958, 965, 1720, 1873
BseSI GKGCMC 1 cut(s) 1802
BseXI GCAGC 4 cut(s) 1468, 1523, 1573, 2135
Bsh1236I CGCG 1 cut(s) 1669
Bsh1285I CGRYCG 1 cut(s) 180
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 1137
BsiHKCI CYCGRG 3 cut(s) 188, 383, 1953
BsiSI CCGG 5 cut(s) 98, 105, 163, 1749, 1796
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 5 cut(s) 104, 878, 1292, 1321, 1614
BsmAI GTCTC 3 cut(s) 272, 293, 1853
BsmBI CGTCTC 1 cut(s) 272
BsmFI GGGAC 1 cut(s) 265
Bso31I GGTCTC 1 cut(s) 293
BsoBI CYCGRG 3 cut(s) 188, 383, 1953
Bsp119I TTCGAA 1 cut(s) 687
Bsp1286I GDGCHC 2 cut(s) 1137, 1802
Bsp1407I TGTACA 1 cut(s) 623
Bsp143I GATC 7 cut(s) 148, 177, 454, 506, 868, 1387, 1660
BspACI CCGC 4 cut(s) 108, 199, 1520, 1669
BspCNI CTCAG 1 cut(s) 1099
BspFNI CGCG 1 cut(s) 1669
BspLI GGNNCC 6 cut(s) 127, 870, 1606, 1747, 1980, 2053
BspMI ACCTGC 1 cut(s) 1202
BspPI GGATC 6 cut(s) 449, 514, 863, 876, 1382, 1655
BspT104I TTCGAA 1 cut(s) 687
BspTNI GGTCTC 1 cut(s) 293
BsrDI GCAATG 1 cut(s) 1074
BsrGI TGTACA 1 cut(s) 623
BsrI ACTGG 6 cut(s) 614, 763, 958, 965, 1720, 1873
BssECI CCNNGG 8 cut(s) 96, 303, 1034, 1090, 1556, 1741, 1845, 1920
BssMI GATC 7 cut(s) 148, 177, 454, 506, 868, 1387, 1660
BssNAI GTATAC 1 cut(s) 363
BssSI CACGAG 1 cut(s) 1515
BssT1I CCWWGG 4 cut(s) 303, 1090, 1845, 1920
Bst1107I GTATAC 1 cut(s) 363
Bst2BI CACGAG 1 cut(s) 1515
Bst2UI CCWGG 5 cut(s) 978, 1035, 1557, 1629, 1742
BstAPI GCANNNNNTGC 3 cut(s) 794, 1421, 1517
BstAUI TGTACA 1 cut(s) 623
BstBI TTCGAA 1 cut(s) 687
BstC8I GCNNGC 1 cut(s) 945
BstDEI CTNAG 3 cut(s) 1107, 1986, 2073
BstF5I GGATG 8 cut(s) 240, 252, 637, 737, 1330, 1535, 1678, 1779
BstFNI CGCG 1 cut(s) 1669
BstHHI GCGC 1 cut(s) 1669
BstKTI GATC 7 cut(s) 151, 180, 457, 509, 871, 1390, 1663
BstMAI GTCTC 3 cut(s) 272, 293, 1853
BstMBI GATC 7 cut(s) 148, 177, 454, 506, 868, 1387, 1660
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 7 cut(s) 125, 794, 1262, 1271, 1421, 1517, 1561
BstNI CCWGG 5 cut(s) 978, 1035, 1557, 1629, 1742
BstNSI RCATGY 3 cut(s) 604, 920, 947
BstSCI CCNGG 8 cut(s) 96, 976, 1033, 1555, 1627, 1740, 1748, 1795
BstSLI GKGCMC 1 cut(s) 1802
BstUI CGCG 1 cut(s) 1669
BstV1I GCAGC 4 cut(s) 1468, 1523, 1573, 2135
BstV2I GAAGAC 3 cut(s) 548, 1639, 1676
BstX2I RGATCY 3 cut(s) 454, 868, 1387
BstXI CCANNNNNNTGG 1 cut(s) 1898
BstYI RGATCY 3 cut(s) 454, 868, 1387
BstZ17I GTATAC 1 cut(s) 363
BsuI GTATCC 1 cut(s) 1867
BtsCI GGATG 8 cut(s) 240, 252, 637, 737, 1330, 1535, 1678, 1779
BtsIMutI CAGTG 6 cut(s) 642, 756, 1342, 1431, 1943, 1980
BveI ACCTGC 1 cut(s) 1202
Cac8I GCNNGC 1 cut(s) 945
CaiI CAGNNNCTG 1 cut(s) 536
CfoI GCGC 1 cut(s) 1669
Cfr13I GGNCC 2 cut(s) 126, 880
CseI GACGC 1 cut(s) 289
CsiI ACCWGGT 1 cut(s) 976
Csp6I GTAC 5 cut(s) 624, 1592, 1960, 2082, 2242
CspCI CAANNNNNGTGG 4 cut(s) 292, 327, 745, 780
CviQI GTAC 5 cut(s) 624, 1592, 1960, 2082, 2242
DdeI CTNAG 3 cut(s) 1107, 1986, 2073
DpnI GATC 7 cut(s) 150, 179, 456, 508, 870, 1389, 1662
DpnII GATC 7 cut(s) 148, 177, 454, 506, 868, 1387, 1660
DriI GACNNNNNGTC 1 cut(s) 258
EaeI YGGCCR 2 cut(s) 117, 1934
Eam1105I GACNNNNNGTC 1 cut(s) 258
Ecl136II GAGCTC 1 cut(s) 1135
Eco130I CCWWGG 4 cut(s) 303, 1090, 1845, 1920
Eco24I GRGCYC 1 cut(s) 1137
Eco31I GGTCTC 1 cut(s) 293
Eco53kI GAGCTC 1 cut(s) 1135
Eco57I CTGAAG 4 cut(s) 360, 392, 639, 1709
Eco88I CYCGRG 3 cut(s) 188, 383, 1953
EcoICRI GAGCTC 1 cut(s) 1135
EcoO109I RGGNCCY 1 cut(s) 126
EcoRI GAATTC 1 cut(s) 1448
EcoRII CCWGG 5 cut(s) 976, 1033, 1555, 1627, 1740
EcoT14I CCWWGG 4 cut(s) 303, 1090, 1845, 1920
EcoT38I GRGCYC 1 cut(s) 1137
ErhI CCWWGG 4 cut(s) 303, 1090, 1845, 1920
Esp3I CGTCTC 1 cut(s) 272
FaqI GGGAC 1 cut(s) 265
FauI CCCGC 1 cut(s) 1662
FbaI TGATCA 1 cut(s) 148
FblI GTMKAC 2 cut(s) 362, 481
Fnu4HI GCNGC 4 cut(s) 1457, 1512, 1562, 2124
FokI GGATG 8 cut(s) 247, 259, 644, 744, 1337, 1542, 1685, 1766
FriOI GRGCYC 1 cut(s) 1137
Fsp4HI GCNGC 4 cut(s) 1457, 1512, 1562, 2124
FspBI CTAG 5 cut(s) 255, 443, 773, 1275, 1383
GlaI GCGC 1 cut(s) 1668
GluI GCNGC 4 cut(s) 1457, 1512, 1562, 2124
GsuI CTGGAG 2 cut(s) 1017, 1737
HapII CCGG 5 cut(s) 98, 105, 163, 1749, 1796
HgaI GACGC 1 cut(s) 289
HhaI GCGC 1 cut(s) 1669
Hin6I GCGC 1 cut(s) 1667
HinP1I GCGC 1 cut(s) 1667
HincII GTYRAC 1 cut(s) 2092
HindII GTYRAC 1 cut(s) 2092
HindIII AAGCTT 1 cut(s) 1586
HpaII CCGG 5 cut(s) 98, 105, 163, 1749, 1796
HphI GGTGA 5 cut(s) 80, 335, 1240, 2200, 2248
Hpy166II GTNNAC 6 cut(s) 185, 363, 482, 624, 2092, 2204
Hpy8I GTNNAC 6 cut(s) 185, 363, 482, 624, 2092, 2204
HpyAV CCTTC 8 cut(s) 139, 148, 302, 320, 335, 893, 1075, 1218
HpyCH4IV ACGT 2 cut(s) 84, 1788
HpyF10VI GCNNNNNNNGC 7 cut(s) 125, 794, 1262, 1271, 1421, 1517, 1561
HpyF3I CTNAG 3 cut(s) 1107, 1986, 2073
HpySE526I ACGT 2 cut(s) 84, 1788
HspAI GCGC 1 cut(s) 1667
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 7 cut(s) 148, 177, 454, 506, 868, 1387, 1660
LmnI GCTCC 6 cut(s) 1140, 1508, 1610, 1745, 2020, 2051
Lsp1109I GCAGC 4 cut(s) 1468, 1523, 1573, 2135
LweI GCATC 9 cut(s) 582, 622, 1051, 1160, 1373, 1403, 1424, 1937, 2204
MabI ACCWGGT 1 cut(s) 976
MaeI CTAG 5 cut(s) 255, 443, 773, 1275, 1383
MaeII ACGT 2 cut(s) 84, 1788
MaeIII GTNAC 8 cut(s) 91, 649, 675, 854, 956, 1547, 1804, 2005
MalI GATC 7 cut(s) 150, 179, 456, 508, 870, 1389, 1662
MboI GATC 7 cut(s) 148, 177, 454, 506, 868, 1387, 1660
MboII GAAGA 8 cut(s) 41, 398, 553, 601, 1644, 1676, 1702, 1907
MflI RGATCY 3 cut(s) 454, 868, 1387
MhlI GDGCHC 2 cut(s) 1137, 1802
MlsI TGGCCA 2 cut(s) 119, 1936
MluNI TGGCCA 2 cut(s) 119, 1936
MlyI GAGTC 5 cut(s) 152, 998, 1052, 1061, 1737
MmeI TCCRAC 1 cut(s) 1744
Mox20I TGGCCA 2 cut(s) 119, 1936
MroXI GAANNNNTTC 1 cut(s) 1115
MscI TGGCCA 2 cut(s) 119, 1936
MseI TTAA 6 cut(s) 222, 816, 906, 1173, 1580, 1734
MslI CAYNNNNRTG 4 cut(s) 321, 576, 1125, 1896
Msp20I TGGCCA 2 cut(s) 119, 1936
MspA1I CMGCKG 1 cut(s) 1352
MspI CCGG 5 cut(s) 98, 105, 163, 1749, 1796
MspR9I CCNGG 8 cut(s) 98, 978, 1035, 1557, 1629, 1742, 1750, 1797
MvaI CCWGG 5 cut(s) 978, 1035, 1557, 1629, 1742
MvnI CGCG 1 cut(s) 1669
MwoI GCNNNNNNNGC 7 cut(s) 125, 794, 1262, 1271, 1421, 1517, 1561
NciI CCSGG 3 cut(s) 98, 1750, 1797
NdeII GATC 7 cut(s) 148, 177, 454, 506, 868, 1387, 1660
NlaIV GGNNCC 6 cut(s) 127, 870, 1606, 1747, 1980, 2053
NmuCI GTSAC 3 cut(s) 649, 854, 1547
NspI RCATGY 3 cut(s) 604, 920, 947
NspV TTCGAA 1 cut(s) 687
PaeI GCATGC 1 cut(s) 947
PaeR7I CTCGAG 2 cut(s) 188, 383
PciI ACATGT 1 cut(s) 600
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 1115
PfeI GAWTC 7 cut(s) 350, 379, 752, 1102, 1699, 2069, 2162
PkrI GCNGC 4 cut(s) 1458, 1513, 1563, 2125
Ple19I CGATCG 1 cut(s) 180
PleI GAGTC 5 cut(s) 151, 998, 1051, 1060, 1736
PpsI GAGTC 5 cut(s) 151, 998, 1051, 1060, 1736
PscI ACATGT 1 cut(s) 600
PsiI TTATAA 2 cut(s) 1358, 1428
Psp124BI GAGCTC 1 cut(s) 1137
Psp1406I AACGTT 1 cut(s) 84
Psp6I CCWGG 5 cut(s) 976, 1033, 1555, 1627, 1740
PspGI CCWGG 5 cut(s) 976, 1033, 1555, 1627, 1740
PspN4I GGNNCC 6 cut(s) 127, 870, 1606, 1747, 1980, 2053
PspPI GGNCC 2 cut(s) 126, 880
PspXI VCTCGAGB 1 cut(s) 188
PstNI CAGNNNCTG 1 cut(s) 536
PsuI RGATCY 3 cut(s) 454, 868, 1387
PvuI CGATCG 1 cut(s) 180
PvuII CAGCTG 1 cut(s) 1352
RsaI GTAC 5 cut(s) 625, 1593, 1961, 2083, 2243
RsaNI GTAC 5 cut(s) 624, 1592, 1960, 2082, 2242
RseI CAYNNNNRTG 4 cut(s) 321, 576, 1125, 1896
SacI GAGCTC 1 cut(s) 1137
SaqAI TTAA 6 cut(s) 222, 816, 906, 1173, 1580, 1734
SatI GCNGC 4 cut(s) 1457, 1512, 1562, 2124
Sau3AI GATC 7 cut(s) 148, 177, 454, 506, 868, 1387, 1660
Sau96I GGNCC 2 cut(s) 126, 880
SchI GAGTC 5 cut(s) 152, 998, 1052, 1061, 1737
ScrFI CCNGG 8 cut(s) 98, 978, 1035, 1557, 1629, 1742, 1750, 1797
SduI GDGCHC 2 cut(s) 1137, 1802
SexAI ACCWGGT 1 cut(s) 976
SfaNI GCATC 9 cut(s) 582, 622, 1051, 1160, 1373, 1403, 1424, 1937, 2204
SfiI GGCCNNNNNGGCC 1 cut(s) 125
Sfr274I CTCGAG 2 cut(s) 188, 383
SfuI TTCGAA 1 cut(s) 687
SlaI CTCGAG 2 cut(s) 188, 383
SmiMI CAYNNNNRTG 4 cut(s) 321, 576, 1125, 1896
SmlI CTYRAG 3 cut(s) 188, 383, 1612
SmoI CTYRAG 3 cut(s) 188, 383, 1612
SpeI ACTAGT 1 cut(s) 254
SphI GCATGC 1 cut(s) 947
SsiI CCGC 4 cut(s) 108, 199, 1520, 1669
SspI AATATT 1 cut(s) 814
SspMI CTAG 5 cut(s) 255, 443, 773, 1275, 1383
SstI GAGCTC 1 cut(s) 1137
StyD4I CCNGG 8 cut(s) 96, 976, 1033, 1555, 1627, 1740, 1748, 1795
StyI CCWWGG 4 cut(s) 303, 1090, 1845, 1920
TaiI ACGT 2 cut(s) 87, 1791
TaqI TCGA 5 cut(s) 189, 384, 687, 1197, 2140
TatI WGTACW 3 cut(s) 623, 1591, 2081
TfiI GAWTC 7 cut(s) 350, 379, 752, 1102, 1699, 2069, 2162
Tru1I TTAA 6 cut(s) 222, 816, 906, 1173, 1580, 1734
Tru9I TTAA 6 cut(s) 222, 816, 906, 1173, 1580, 1734
TscAI CASTG 6 cut(s) 649, 763, 1342, 1438, 1950, 1980
TseFI GTSAC 3 cut(s) 649, 854, 1547
TseI GCWGC 4 cut(s) 1456, 1511, 1561, 2123
Tsp45I GTSAC 3 cut(s) 649, 854, 1547
TspGWI ACGGA 1 cut(s) 1337
TspRI CASTG 6 cut(s) 649, 763, 1342, 1438, 1950, 1980
XapI RAATTY 2 cut(s) 830, 1448
XceI RCATGY 3 cut(s) 604, 920, 947
XcmI CCANNNNNNNNNTGG 1 cut(s) 1322
XhoI CTCGAG 2 cut(s) 188, 383
XmiI GTMKAC 2 cut(s) 362, 481
XmnI GAANNNNTTC 1 cut(s) 1115
XspI CTAG 5 cut(s) 255, 443, 773, 1275, 1383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.