AT1G59710

Domain of unknown function (DUF569)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005974
Physical Location & Seq
Forward (+)
21938367 .. 21940184
1818 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G59710.3

Sequence Viewer

Length: 903 bp
ATGGAGATTTTTCAAAAGGCCAAAGCCGTTCGTCTACGGAGCCATCACGACAAGTACCTAGTCGCCGATGAAGACGAAGAATCTGTAACTCAAGAACGTAACGGCTCAGCAGGTGCAGCCAAATGGACCGTTGAGATAATCCCAGGCTCAACAAATCTCATCCGTCTCAAAAGCGCTTATGGCAAATACTTAACAGCGTCTAACAAACCGTTTCTTCTTGGAGCCACCGGAAAAAAAGTTCTACAGACTAAACCTAGTCGTCTTGACTCGTCTTTAGCTTGGGAACCGATTAGAGATTCTGCTTTGGTTAAGCTCAAGACTCGTTATGGTAACTTCCTTCGTGGTAACGGTGGTTTACCTCCCTGGAGAAACTCTGTTACTCATGATATTCCTCATAGATCGGCCACTCAAGAATGGGTACTTTGGCATATAGATGTCGTTGAGATCCTCCCTGCAAACGCAAACTCCGATCACCACCACCAGCACCTGCAGCTGCAGCAACCTCCGTCGCCGCTTCATCATTCTGATTCTCTTGATTTCACACCTGGATCTCCTTCCAGATCTGACCGTTTCTTTAGACAAGAGTCAACGGATTCACTGGCGGTTGGATCGCCGCCGAAATCGGAAGGGAGAGTGATATACTACCATGTGGCGGATGATGATGATGATGTTGAAGATGACTCTGTTGAGGTAAGTTCGTTTACTTTCAAAGGAAATGGTGTTGAAGAGCTGACTATGAGATTGAAAGAAGAGAGCAATGTCGAAGACGTTATCGTTTGTACTCGTAGTCCGTTGAACGGGAAGCTTTTCCCATTGCGTCTTCAACTTCCACCTAACAATGCTGACATGACTGTTGTCTTAGTGCCAAAATCATCAAAAATAGCAGAAAAGTTCATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF569

Protein Analysis

300

Amino Acids

33.58

Weight (kDa)

6.46

Isoelectric Point (pI)

50.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF569 PF04601 1 - 142 1.4e-78 Beta-trefoil domain of unknown function (DUF569)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 101, 495
Acc36I ACCTGC 2 cut(s) 101, 495
AccI GTMKAC 1 cut(s) 34
AciI CCGC 4 cut(s) 512, 602, 614, 653
AclWI GGATC 3 cut(s) 439, 556, 616
AcoI YGGCCR 1 cut(s) 402
AfaI GTAC 3 cut(s) 56, 420, 781
AfeI AGCGCT 1 cut(s) 175
AfiI CCNNNNNNNGG 2 cut(s) 652, 797
AgsI TTSAA 7 cut(s) 14, 674, 709, 725, 745, 796, 824
AjnI CCWGG 3 cut(s) 142, 362, 544
AloI GAACNNNNNNTCC 2 cut(s) 222, 254
AluBI AGCT 5 cut(s) 278, 313, 493, 730, 805
AluI AGCT 5 cut(s) 278, 313, 493, 730, 805
Alw26I GTCTC 1 cut(s) 170
AlwI GGATC 3 cut(s) 439, 556, 616
AlwNI CAGNNNCTG 1 cut(s) 487
Aor51HI AGCGCT 1 cut(s) 175
AoxI GGCC 2 cut(s) 18, 402
ApeKI GCWGC 4 cut(s) 116, 490, 493, 496
Asp700I GAANNNNTTC 1 cut(s) 806
AspLEI GCGC 1 cut(s) 176
AspS9I GGNCC 1 cut(s) 126
AsuHPI GGTGA 1 cut(s) 464
AvaII GGWCC 1 cut(s) 126
BbsI GAAGAC 3 cut(s) 78, 771, 812
BbvI GCAGC 4 cut(s) 128, 480, 502, 508
BccI CCATC 1 cut(s) 51
BceAI ACGGC 2 cut(s) 11, 118
BciT130I CCWGG 3 cut(s) 144, 364, 546
BcoDI GTCTC 1 cut(s) 170
BfaI CTAG 2 cut(s) 59, 255
BfmI CTRYAG 3 cut(s) 242, 488, 494
BfoI RGCGCY 1 cut(s) 177
BfuAI ACCTGC 2 cut(s) 101, 495
BglII AGATCT 1 cut(s) 560
BisI GCNGC 6 cut(s) 117, 491, 494, 497, 512, 614
BlpI GCTNAGC 1 cut(s) 106
BlsI GCNGC 6 cut(s) 118, 492, 495, 498, 513, 615
Bme1390I CCNGG 3 cut(s) 144, 364, 546
Bme18I GGWCC 1 cut(s) 126
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 3 cut(s) 41, 223, 285
BmrFI CCNGG 3 cut(s) 144, 364, 546
BoxI GACNNNNGTC 2 cut(s) 583, 854
BpiI GAAGAC 3 cut(s) 78, 771, 812
BpmI CTGGAG 1 cut(s) 385
Bpu1102I GCTNAGC 1 cut(s) 106
BpuEI CTTGAG 3 cut(s) 75, 299, 393
BsaJI CCNNGG 2 cut(s) 142, 362
BsaWI WCCGGW 1 cut(s) 227
BsaXI ACNNNNNCTCC 2 cut(s) 449, 479
Bsc4I CCNNNNNNNGG 2 cut(s) 652, 797
Bse1I ACTGG 1 cut(s) 603
Bse3DI GCAATG 2 cut(s) 763, 812
BseBI CCWGG 3 cut(s) 144, 364, 546
BseDI CCNNGG 2 cut(s) 142, 362
BseGI GGATG 2 cut(s) 159, 661
BseLI CCNNNNNNNGG 2 cut(s) 652, 797
BseMI GCAATG 2 cut(s) 763, 812
BseMII CTCAG 1 cut(s) 120
BseNI ACTGG 1 cut(s) 603
BseXI GCAGC 4 cut(s) 128, 480, 502, 508
BsgI GTGCAG 1 cut(s) 135
BshFI GGCC 2 cut(s) 20, 404
BsiSI CCGG 1 cut(s) 228
BslI CCNNNNNNNGG 2 cut(s) 652, 797
BsmAI GTCTC 1 cut(s) 170
BsmBI CGTCTC 1 cut(s) 170
BsnI GGCC 2 cut(s) 20, 404
Bsp143I GATC 6 cut(s) 398, 444, 469, 548, 560, 608
Bsp1720I GCTNAGC 1 cut(s) 106
BspACI CCGC 4 cut(s) 512, 602, 614, 653
BspANI GGCC 2 cut(s) 20, 404
BspCNI CTCAG 1 cut(s) 119
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 3 cut(s) 41, 223, 285
BspMAI CTGCAG 2 cut(s) 492, 498
BspMI ACCTGC 2 cut(s) 101, 495
BspPI GGATC 3 cut(s) 439, 556, 616
BspQI GCTCTTC 1 cut(s) 720
BsrDI GCAATG 2 cut(s) 763, 812
BsrI ACTGG 1 cut(s) 603
BssECI CCNNGG 2 cut(s) 142, 362
BssMI GATC 6 cut(s) 398, 444, 469, 548, 560, 608
Bst2UI CCWGG 3 cut(s) 144, 364, 546
Bst4CI ACNGT 5 cut(s) 130, 210, 350, 569, 853
Bst6I CTCTTC 2 cut(s) 720, 744
BstDEI CTNAG 2 cut(s) 106, 859
BstF5I GGATG 2 cut(s) 159, 661
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 6 cut(s) 401, 447, 472, 551, 563, 611
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 6 cut(s) 398, 444, 469, 548, 560, 608
BstMWI GCNNNNNNNGC 4 cut(s) 116, 180, 490, 496
BstNI CCWGG 3 cut(s) 144, 364, 546
BstPAI GACNNNNGTC 2 cut(s) 583, 854
BstSCI CCNGG 3 cut(s) 142, 362, 544
BstSFI CTRYAG 3 cut(s) 242, 488, 494
BstV1I GCAGC 4 cut(s) 128, 480, 502, 508
BstV2I GAAGAC 3 cut(s) 78, 771, 812
BstX2I RGATCY 3 cut(s) 444, 548, 560
BstYI RGATCY 3 cut(s) 444, 548, 560
BsuRI GGCC 2 cut(s) 20, 404
BtsCI GGATG 2 cut(s) 159, 661
BtsIMutI CAGTG 1 cut(s) 596
BveI ACCTGC 2 cut(s) 101, 495
CaiI CAGNNNCTG 1 cut(s) 487
CciI TCATGA 1 cut(s) 382
CfoI GCGC 1 cut(s) 176
Cfr13I GGNCC 1 cut(s) 126
CseI GACGC 2 cut(s) 186, 806
Csp6I GTAC 3 cut(s) 55, 419, 780
CviAII CATG 3 cut(s) 383, 647, 847
CviQI GTAC 3 cut(s) 55, 419, 780
DdeI CTNAG 2 cut(s) 106, 859
DpnI GATC 6 cut(s) 400, 446, 471, 550, 562, 610
DpnII GATC 6 cut(s) 398, 444, 469, 548, 560, 608
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 2 cut(s) 720, 744
EarI CTCTTC 2 cut(s) 720, 744
EciI GGCGGA 1 cut(s) 668
Eco47I GGWCC 1 cut(s) 126
Eco47III AGCGCT 1 cut(s) 175
EcoRII CCWGG 3 cut(s) 142, 362, 544
Esp3I CGTCTC 1 cut(s) 170
FaeI CATG 3 cut(s) 386, 650, 850
FatI CATG 3 cut(s) 382, 646, 846
FblI GTMKAC 1 cut(s) 34
Fnu4HI GCNGC 6 cut(s) 117, 491, 494, 497, 512, 614
FokI GGATG 2 cut(s) 146, 668
Fsp4HI GCNGC 6 cut(s) 117, 491, 494, 497, 512, 614
FspBI CTAG 2 cut(s) 59, 255
GlaI GCGC 1 cut(s) 175
GluI GCNGC 6 cut(s) 117, 491, 494, 497, 512, 614
GsuI CTGGAG 1 cut(s) 385
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 2 cut(s) 20, 404
HapII CCGG 1 cut(s) 228
HgaI GACGC 2 cut(s) 186, 806
HhaI GCGC 1 cut(s) 176
Hin1II CATG 3 cut(s) 386, 650, 850
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HincII GTYRAC 1 cut(s) 588
HindII GTYRAC 1 cut(s) 588
HindIII AAGCTT 1 cut(s) 803
HinfI GANTC 8 cut(s) 80, 266, 296, 319, 527, 584, 593, 680
HpaII CCGG 1 cut(s) 228
HphI GGTGA 1 cut(s) 464
Hpy166II GTNNAC 4 cut(s) 35, 356, 588, 702
Hpy188I TCNGA 4 cut(s) 469, 526, 565, 625
Hpy188III TCNNGA 8 cut(s) 47, 92, 263, 316, 383, 410, 533, 558
Hpy8I GTNNAC 4 cut(s) 35, 356, 588, 702
Hpy99I CGWCG 1 cut(s) 511
HpyAV CCTTC 3 cut(s) 347, 564, 620
HpyCH4III ACNGT 5 cut(s) 130, 210, 350, 569, 853
HpyCH4IV ACGT 2 cut(s) 97, 768
HpyCH4V TGCA 4 cut(s) 116, 455, 490, 496
HpyF10VI GCNNNNNNNGC 4 cut(s) 116, 180, 490, 496
HpyF3I CTNAG 2 cut(s) 106, 859
HpySE526I ACGT 2 cut(s) 97, 768
Hsp92II CATG 3 cut(s) 386, 650, 850
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 6 cut(s) 398, 444, 469, 548, 560, 608
LguI GCTCTTC 1 cut(s) 720
LmnI GCTCC 2 cut(s) 39, 221
Lsp1109I GCAGC 4 cut(s) 128, 480, 502, 508
MaeI CTAG 2 cut(s) 59, 255
MaeII ACGT 2 cut(s) 97, 768
MaeIII GTNAC 5 cut(s) 85, 98, 329, 344, 376
MalI GATC 6 cut(s) 400, 446, 471, 550, 562, 610
MboI GATC 6 cut(s) 398, 444, 469, 548, 560, 608
MboII GAAGA 8 cut(s) 83, 89, 206, 686, 737, 761, 776, 812
MflI RGATCY 3 cut(s) 444, 548, 560
MlyI GAGTC 4 cut(s) 260, 313, 593, 674
MmeI TCCRAC 1 cut(s) 586
MnlI CCTC 5 cut(s) 369, 402, 458, 513, 682
MroXI GAANNNNTTC 1 cut(s) 806
MseI TTAA 2 cut(s) 191, 309
MslI CAYNNNNRTG 1 cut(s) 432
MspA1I CMGCKG 1 cut(s) 493
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 3 cut(s) 144, 364, 546
MvaI CCWGG 3 cut(s) 144, 364, 546
MwoI GCNNNNNNNGC 4 cut(s) 116, 180, 490, 496
NdeII GATC 6 cut(s) 398, 444, 469, 548, 560, 608
NlaIII CATG 3 cut(s) 386, 650, 850
NlaIV GGNNCC 3 cut(s) 41, 223, 285
PagI TCATGA 1 cut(s) 382
PaqCI CACCTGC 2 cut(s) 101, 495
PciSI GCTCTTC 1 cut(s) 720
PcsI WCGNNNNNNNCGW 1 cut(s) 465
PdmI GAANNNNTTC 1 cut(s) 806
PfeI GAWTC 4 cut(s) 80, 296, 527, 593
PkrI GCNGC 6 cut(s) 118, 492, 495, 498, 513, 615
PleI GAGTC 4 cut(s) 260, 313, 592, 674
PpsI GAGTC 4 cut(s) 260, 313, 592, 674
PshAI GACNNNNGTC 2 cut(s) 583, 854
Psp6I CCWGG 3 cut(s) 142, 362, 544
PspGI CCWGG 3 cut(s) 142, 362, 544
PspN4I GGNNCC 3 cut(s) 41, 223, 285
PspPI GGNCC 1 cut(s) 126
PstI CTGCAG 2 cut(s) 492, 498
PstNI CAGNNNCTG 1 cut(s) 487
PsuI RGATCY 3 cut(s) 444, 548, 560
PvuII CAGCTG 1 cut(s) 493
RsaI GTAC 3 cut(s) 56, 420, 781
RsaNI GTAC 3 cut(s) 55, 419, 780
RseI CAYNNNNRTG 1 cut(s) 432
SapI GCTCTTC 1 cut(s) 720
SaqAI TTAA 2 cut(s) 191, 309
SatI GCNGC 6 cut(s) 117, 491, 494, 497, 512, 614
Sau3AI GATC 6 cut(s) 398, 444, 469, 548, 560, 608
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 4 cut(s) 260, 313, 593, 674
ScrFI CCNGG 3 cut(s) 144, 364, 546
SfcI CTRYAG 3 cut(s) 242, 488, 494
SinI GGWCC 1 cut(s) 126
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 3 cut(s) 90, 314, 408
SmoI CTYRAG 3 cut(s) 90, 314, 408
SsiI CCGC 4 cut(s) 512, 602, 614, 653
SspMI CTAG 2 cut(s) 59, 255
StyD4I CCNGG 3 cut(s) 142, 362, 544
TaaI ACNGT 5 cut(s) 130, 210, 350, 569, 853
TaiI ACGT 2 cut(s) 100, 771
TaqI TCGA 1 cut(s) 762
TatI WGTACW 1 cut(s) 779
TauI GCSGC 2 cut(s) 514, 616
TfiI GAWTC 4 cut(s) 80, 296, 527, 593
Tru1I TTAA 2 cut(s) 191, 309
Tru9I TTAA 2 cut(s) 191, 309
TscAI CASTG 1 cut(s) 603
TseI GCWGC 4 cut(s) 116, 490, 493, 496
TspDTI ATGAA 3 cut(s) 84, 506, 883
TspGWI ACGGA 5 cut(s) 52, 152, 495, 605, 780
TspRI CASTG 1 cut(s) 603
VpaK11BI GGWCC 1 cut(s) 126
XmiI GTMKAC 1 cut(s) 34
XmnI GAANNNNTTC 1 cut(s) 806
XspI CTAG 2 cut(s) 59, 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.