MD02G1256000.v1.1

Domain of unknown function (DUF569)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005974
Physical Location & Seq
Reverse (-)
30794351 .. 30794746
396 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1256000.v1.1.491

Sequence Viewer

Length: 396 bp
ATGACTGGCAATAAAGTCATCCAAGCAAAGTTAGACAAGCTCATCGATTGGAAGTTCGAGTGGGAGCCCATACGCGAAGGGTTCCAGGTTAAACTGCGAACGTGGTGCGGCACGTACTTGCACTCCAATGGTGGGCCGCCGCCGTGGCGAAACTCGCTCACTCACGACGACCCCACCACGACATCAACCCAGAACTGGATTCTGTGGGACGTTTGGAAGACAGAAGTTCCGGTGTCAGAGTCATTGTTGTCGCAACAGTCAAGTTTTTTGTCGTTCTCCGACGAGCTTAACGGGCAGCCCATTTCAGTGATTTCAACCAAGTCGCCTAATTCAGAACCGTCGTCGAAAAAGCTAGCTAAGTCTCTGAAGTCGTCGTCCAATAGTATGGCTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF569

Protein Analysis

132

Amino Acids

14.78

Weight (kDa)

8.73

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF569 PF04601 1 - 69 5.3e-27 Beta-trefoil domain of unknown function (DUF569)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 75
AciI CCGC 3 cut(s) 108, 137, 140
AcuI CTGAAG 1 cut(s) 386
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 2 cut(s) 132, 195
AgsI TTSAA 1 cut(s) 315
AjnI CCWGG 1 cut(s) 84
AluBI AGCT 4 cut(s) 40, 286, 352, 356
AluI AGCT 4 cut(s) 40, 286, 352, 356
Alw26I GTCTC 1 cut(s) 366
AoxI GGCC 1 cut(s) 134
ApeKI GCWGC 1 cut(s) 295
AspS9I GGNCC 1 cut(s) 134
AsuNHI GCTAGC 1 cut(s) 352
BanII GRGCYC 1 cut(s) 69
BbsI GAAGAC 1 cut(s) 224
BbvI GCAGC 1 cut(s) 307
BceAI ACGGC 1 cut(s) 127
BcgI CGANNNNNNTGC 2 cut(s) 87, 121
BciT130I CCWGG 1 cut(s) 86
BcoDI GTCTC 1 cut(s) 366
BfaI CTAG 1 cut(s) 353
BglI GCCNNNNNGGC 1 cut(s) 145
BisI GCNGC 4 cut(s) 109, 137, 140, 296
BlsI GCNGC 4 cut(s) 110, 138, 141, 297
Bme1390I CCNGG 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 134
BmiI GGNNCC 2 cut(s) 66, 83
BmrFI CCNGG 1 cut(s) 86
BmtI GCTAGC 1 cut(s) 356
BpiI GAAGAC 1 cut(s) 224
Bsa29I ATCGAT 1 cut(s) 45
BsaAI YACGTR 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 143
BsaWI WCCGGW 1 cut(s) 229
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 195
Bse1I ACTGG 2 cut(s) 10, 200
BseBI CCWGG 1 cut(s) 86
BseCI ATCGAT 1 cut(s) 45
BseDI CCNNGG 1 cut(s) 143
BseGI GGATG 1 cut(s) 18
BseLI CCNNNNNNNGG 2 cut(s) 132, 195
BseNI ACTGG 2 cut(s) 10, 200
BseXI GCAGC 1 cut(s) 307
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 136
BshVI ATCGAT 1 cut(s) 45
BsiSI CCGG 1 cut(s) 230
BslFI GGGAC 1 cut(s) 221
BslI CCNNNNNNNGG 2 cut(s) 132, 195
BsmAI GTCTC 1 cut(s) 366
BsmFI GGGAC 1 cut(s) 221
BsnI GGCC 1 cut(s) 136
Bsp1286I GDGCHC 1 cut(s) 69
BspACI CCGC 3 cut(s) 108, 137, 140
BspANI GGCC 1 cut(s) 136
BspDI ATCGAT 1 cut(s) 45
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 2 cut(s) 66, 83
BspOI GCTAGC 1 cut(s) 356
BsrI ACTGG 2 cut(s) 10, 200
BssECI CCNNGG 1 cut(s) 143
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 2 cut(s) 258, 339
BstBAI YACGTR 1 cut(s) 114
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 2 cut(s) 357, 390
BstDSI CCRYGG 1 cut(s) 143
BstF5I GGATG 1 cut(s) 18
BstFNI CGCG 1 cut(s) 75
BstMAI GTCTC 1 cut(s) 366
BstMWI GCNNNNNNNGC 3 cut(s) 145, 154, 292
BstNI CCWGG 1 cut(s) 86
BstSCI CCNGG 1 cut(s) 84
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 307
BstV2I GAAGAC 1 cut(s) 224
BstXI CCANNNNNNTGG 1 cut(s) 385
Bsu15I ATCGAT 1 cut(s) 45
BsuRI GGCC 1 cut(s) 136
BsuTUI ATCGAT 1 cut(s) 45
BtgI CCRYGG 1 cut(s) 143
BtsCI GGATG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 312
Cac8I GCNNGC 1 cut(s) 354
Cfr13I GGNCC 1 cut(s) 134
ClaI ATCGAT 1 cut(s) 45
Csp6I GTAC 1 cut(s) 115
CviJI RGCY 8 cut(s) 40, 67, 136, 286, 298, 352, 356, 389
CviKI_1 RGCY 8 cut(s) 40, 67, 136, 286, 298, 352, 356, 389
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 2 cut(s) 357, 390
Eco24I GRGCYC 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 84
EcoT38I GRGCYC 1 cut(s) 69
FaiI YATR 2 cut(s) 71, 386
FaqI GGGAC 1 cut(s) 221
Fnu4HI GCNGC 4 cut(s) 109, 137, 140, 296
FokI GGATG 1 cut(s) 5
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 4 cut(s) 109, 137, 140, 296
FspBI CTAG 1 cut(s) 353
GluI GCNGC 4 cut(s) 109, 137, 140, 296
HaeIII GGCC 1 cut(s) 136
HapII CCGG 1 cut(s) 230
HinfI GANTC 2 cut(s) 199, 239
HpaII CCGG 1 cut(s) 230
Hpy188I TCNGA 4 cut(s) 238, 280, 334, 366
Hpy188III TCNNGA 1 cut(s) 164
Hpy99I CGWCG 5 cut(s) 170, 284, 343, 346, 376
HpyAV CCTTC 1 cut(s) 71
HpyCH4III ACNGT 2 cut(s) 258, 339
HpyCH4IV ACGT 3 cut(s) 101, 113, 210
HpyCH4V TGCA 1 cut(s) 121
HpyF10VI GCNNNNNNNGC 3 cut(s) 145, 154, 292
HpyF3I CTNAG 2 cut(s) 357, 390
HpySE526I ACGT 3 cut(s) 101, 113, 210
LmnI GCTCC 1 cut(s) 64
LpnPI CCDG 5 cut(s) 71, 98, 181, 203, 243
Lsp1109I GCAGC 1 cut(s) 307
MaeI CTAG 1 cut(s) 353
MaeII ACGT 3 cut(s) 101, 113, 210
MboII GAAGA 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 1 cut(s) 328
MlyI GAGTC 1 cut(s) 248
MmeI TCCRAC 1 cut(s) 303
MseI TTAA 2 cut(s) 90, 288
MslI CAYNNNNRTG 2 cut(s) 126, 305
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 1 cut(s) 86
MvaI CCWGG 1 cut(s) 86
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 3 cut(s) 145, 154, 292
NheI GCTAGC 1 cut(s) 352
NlaIV GGNNCC 2 cut(s) 66, 83
PfeI GAWTC 1 cut(s) 199
PkrI GCNGC 4 cut(s) 110, 138, 141, 297
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
Ppu21I YACGTR 1 cut(s) 114
Psp6I CCWGG 1 cut(s) 84
PspGI CCWGG 1 cut(s) 84
PspN4I GGNNCC 2 cut(s) 66, 83
PspPI GGNCC 1 cut(s) 134
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
RseI CAYNNNNRTG 2 cut(s) 126, 305
SaqAI TTAA 2 cut(s) 90, 288
SatI GCNGC 4 cut(s) 109, 137, 140, 296
Sau96I GGNCC 1 cut(s) 134
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 1 cut(s) 86
SduI GDGCHC 1 cut(s) 69
SetI ASST 8 cut(s) 42, 90, 104, 116, 213, 288, 354, 358
SmiMI CAYNNNNRTG 2 cut(s) 126, 305
Sse9I AATT 1 cut(s) 328
SsiI CCGC 3 cut(s) 108, 137, 140
SspMI CTAG 1 cut(s) 353
StyD4I CCNGG 1 cut(s) 84
TaaI ACNGT 2 cut(s) 258, 339
TaiI ACGT 3 cut(s) 104, 116, 213
TaqI TCGA 3 cut(s) 45, 57, 344
TasI AATT 1 cut(s) 328
TauI GCSGC 3 cut(s) 111, 139, 142
TfiI GAWTC 1 cut(s) 199
Tru1I TTAA 2 cut(s) 90, 288
Tru9I TTAA 2 cut(s) 90, 288
TscAI CASTG 1 cut(s) 312
TseI GCWGC 1 cut(s) 295
TspRI CASTG 1 cut(s) 312
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.