AT1G61065

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014505
Physical Location & Seq
Reverse (-)
22490221 .. 22491539
1319 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G61065.1

Sequence Viewer

Length: 543 bp
ATGGCTTCCGTGTTGCTACTTCTTCTGGTGTTCGTGTTTGATCTCATTGCTTTTGGTCTTGCTGTTGCTGCAGAGCAACGAAGAACCACCTGGCAAATTTCTAGAGAATCAAGAGATTTGAGCTACTGTGTGTATGACAAGGACATAGCAACGGGTCTCGGAGTCGGTTCTTTCTTGGTCTTATTAGCGAGTCAGCTCTTAATAATGGTGGCGAGCCGATGTTTATGCTGTGGAAGAGCATTGACACCAAGTGGTTCAAGATCTTGGGCAATTTTTCTCTTCATCACCACCTGGGTTTTCTTCTTCATAGCACAAGTGTGTTTACTTGCTGGCTCTGTACGAAACGCTTACCATACAAAGTACCGTGTCTACTTTGGTAACACTTCCCCTTCTTGTCGATCACTGAGGAAAGGCGTGTTTGGGGCTGGAGCAGCATTCATAGTGCTCACGGGGATTGTCTCCGAGCTTTACTATGTGACCCTTTCGAGGGCCAAAGACTTTCAACCTTCAAGAGATCCCGGTATCCGAATGAGTAGCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

180

Amino Acids

19.87

Weight (kDa)

9.6

Isoelectric Point (pI)

35.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 57 - 151 1.1e-28 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61065
fragaria_vesca FvH4_3g04720
prunus_persica Prupe.4G042400_v2.0.a1 Prupe.4G042400_v2.0.a1
pyrus_communis pycom05g29480
rosa_chinensis RchiOBHm_Chr5g0007741
rosa_laevigata RLG00000031483
rosa_multiflora Rmu_sc0005098.1_g000021
rosa_roxburghii Rroxscaffold_1G00068670
rosa_rugosa Rorug04G0429800 Rorug04G0429900 Rorug04G0430000
rosa_samantha Rh5AG058300 Rh5BG055100 Rh5CG064800 Rh5DG054100
rosa_wichuraiana Rw5G006140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 369
AclWI GGATC 1 cut(s) 509
AcsI RAATTY 1 cut(s) 96
AdeI CACNNNGTG 1 cut(s) 251
AfaI GTAC 2 cut(s) 339, 362
AfiI CCNNNNNNNGG 2 cut(s) 486, 487
AgsI TTSAA 3 cut(s) 258, 503, 510
AjnI CCWGG 2 cut(s) 89, 290
AleI CACNNNNGTG 1 cut(s) 316
AluBI AGCT 3 cut(s) 123, 196, 466
AluI AGCT 3 cut(s) 123, 196, 466
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 2 cut(s) 161, 463
AlwI GGATC 1 cut(s) 509
AoxI GGCC 1 cut(s) 489
ApeKI GCWGC 2 cut(s) 68, 431
ApoI RAATTY 1 cut(s) 96
AspS9I GGNCC 1 cut(s) 489
AsuC2I CCSGG 1 cut(s) 519
AsuHPI GGTGA 1 cut(s) 277
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 2 cut(s) 55, 443
BcgI CGANNNNNNTGC 2 cut(s) 207, 241
BciT130I CCWGG 2 cut(s) 91, 292
BciVI GTATCC 1 cut(s) 533
BcnI CCSGG 1 cut(s) 519
BcoDI GTCTC 2 cut(s) 161, 463
BfaI CTAG 1 cut(s) 102
BfmI CTRYAG 2 cut(s) 69, 539
BfuI GTATCC 1 cut(s) 533
BglII AGATCT 1 cut(s) 260
BisI GCNGC 2 cut(s) 69, 432
BlsI GCNGC 2 cut(s) 70, 433
Bme1390I CCNGG 3 cut(s) 91, 292, 519
BmgT120I GGNCC 1 cut(s) 489
BmrFI CCNGG 3 cut(s) 91, 292, 519
BpmI CTGGAG 1 cut(s) 447
BpuMI CCSGG 1 cut(s) 519
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 1 cut(s) 291
Bsc4I CCNNNNNNNGG 2 cut(s) 486, 487
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 2 cut(s) 91, 292
BseDI CCNNGG 1 cut(s) 291
BseLI CCNNNNNNNGG 2 cut(s) 486, 487
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 395
BseXI GCAGC 2 cut(s) 55, 443
BshFI GGCC 1 cut(s) 491
BsiHKAI GWGCWC 1 cut(s) 447
BsiSI CCGG 1 cut(s) 519
BslI CCNNNNNNNGG 2 cut(s) 486, 487
BsmAI GTCTC 2 cut(s) 161, 463
BsmI GAATGC 1 cut(s) 434
BsnI GGCC 1 cut(s) 491
Bso31I GGTCTC 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 447
Bsp143I GATC 4 cut(s) 40, 260, 398, 514
BspANI GGCC 1 cut(s) 491
BspCNI CTCAG 1 cut(s) 396
BspMAI CTGCAG 1 cut(s) 73
BspPI GGATC 1 cut(s) 509
BspQI GCTCTTC 1 cut(s) 229
BspTNI GGTCTC 1 cut(s) 161
BsrDI GCAATG 1 cut(s) 45
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 4 cut(s) 40, 260, 398, 514
Bst2UI CCWGG 2 cut(s) 91, 292
Bst4CI ACNGT 2 cut(s) 128, 365
Bst6I CTCTTC 2 cut(s) 229, 284
BstC8I GCNNGC 2 cut(s) 214, 331
BstDEI CTNAG 1 cut(s) 404
BstKTI GATC 4 cut(s) 43, 263, 401, 517
BstMAI GTCTC 2 cut(s) 161, 463
BstMBI GATC 4 cut(s) 40, 260, 398, 514
BstMWI GCNNNNNNNGC 2 cut(s) 68, 431
BstNI CCWGG 2 cut(s) 91, 292
BstSCI CCNGG 3 cut(s) 89, 290, 517
BstSFI CTRYAG 2 cut(s) 69, 539
BstV1I GCAGC 2 cut(s) 55, 443
BstX2I RGATCY 2 cut(s) 260, 514
BstYI RGATCY 2 cut(s) 260, 514
BsuI GTATCC 1 cut(s) 533
BsuRI GGCC 1 cut(s) 491
BtsIMutI CAGTG 1 cut(s) 401
Cac8I GCNNGC 2 cut(s) 214, 331
Cfr13I GGNCC 1 cut(s) 489
Csp6I GTAC 2 cut(s) 338, 361
CviJI RGCY 9 cut(s) 5, 123, 196, 216, 333, 425, 466, 491, 537
CviKI_1 RGCY 9 cut(s) 5, 123, 196, 216, 333, 425, 466, 491, 537
CviQI GTAC 2 cut(s) 338, 361
DdeI CTNAG 1 cut(s) 404
DpnI GATC 4 cut(s) 42, 262, 400, 516
DpnII GATC 4 cut(s) 40, 260, 398, 514
DraIII CACNNNGTG 1 cut(s) 251
Eam1104I CTCTTC 2 cut(s) 229, 284
EarI CTCTTC 2 cut(s) 229, 284
Eco31I GGTCTC 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 89, 290
FaiI YATR 7 cut(s) 135, 146, 226, 308, 354, 440, 474
FblI GTMKAC 1 cut(s) 369
Fnu4HI GCNGC 2 cut(s) 69, 432
Fsp4HI GCNGC 2 cut(s) 69, 432
FspBI CTAG 1 cut(s) 102
GluI GCNGC 2 cut(s) 69, 432
GsuI CTGGAG 1 cut(s) 447
HaeIII GGCC 1 cut(s) 491
HapII CCGG 1 cut(s) 519
HinfI GANTC 3 cut(s) 107, 162, 190
HpaII CCGG 1 cut(s) 519
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 2 cut(s) 323, 370
Hpy188I TCNGA 3 cut(s) 161, 463, 527
Hpy188III TCNNGA 4 cut(s) 102, 111, 258, 510
Hpy8I GTNNAC 2 cut(s) 323, 370
HpyAV CCTTC 2 cut(s) 399, 516
HpyCH4III ACNGT 2 cut(s) 128, 365
HpyCH4V TGCA 1 cut(s) 71
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 431
HpyF3I CTNAG 1 cut(s) 404
Kzo9I GATC 4 cut(s) 40, 260, 398, 514
LguI GCTCTTC 1 cut(s) 229
LmnI GCTCC 1 cut(s) 428
LpnPI CCDG 8 cut(s) 11, 76, 103, 277, 304, 315, 411, 532
Lsp1109I GCAGC 2 cut(s) 55, 443
MaeI CTAG 1 cut(s) 102
MaeIII GTNAC 2 cut(s) 377, 475
MalI GATC 4 cut(s) 42, 262, 400, 516
MboI GATC 4 cut(s) 40, 260, 398, 514
MboII GAAGA 6 cut(s) 14, 93, 246, 271, 292, 295
MflI RGATCY 2 cut(s) 260, 514
MhlI GDGCHC 1 cut(s) 447
MluCI AATT 2 cut(s) 96, 270
MlyI GAGTC 2 cut(s) 171, 199
MnlI CCTC 2 cut(s) 399, 480
MseI TTAA 1 cut(s) 200
MslI CAYNNNNRTG 1 cut(s) 316
MspI CCGG 1 cut(s) 519
MspR9I CCNGG 3 cut(s) 91, 292, 519
Mva1269I GAATGC 1 cut(s) 434
MvaI CCWGG 2 cut(s) 91, 292
MwoI GCNNNNNNNGC 2 cut(s) 68, 431
NciI CCSGG 1 cut(s) 519
NdeII GATC 4 cut(s) 40, 260, 398, 514
NmuCI GTSAC 1 cut(s) 475
OliI CACNNNNGTG 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 229
PctI GAATGC 1 cut(s) 434
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 2 cut(s) 70, 433
PleI GAGTC 2 cut(s) 170, 198
PpsI GAGTC 2 cut(s) 170, 198
Psp6I CCWGG 2 cut(s) 89, 290
PspGI CCWGG 2 cut(s) 89, 290
PspPI GGNCC 1 cut(s) 489
PstI CTGCAG 1 cut(s) 73
PsuI RGATCY 2 cut(s) 260, 514
RsaI GTAC 2 cut(s) 339, 362
RsaNI GTAC 2 cut(s) 338, 361
RseI CAYNNNNRTG 1 cut(s) 316
SapI GCTCTTC 1 cut(s) 229
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 2 cut(s) 69, 432
Sau3AI GATC 4 cut(s) 40, 260, 398, 514
Sau96I GGNCC 1 cut(s) 489
SchI GAGTC 2 cut(s) 171, 199
ScrFI CCNGG 3 cut(s) 91, 292, 519
SduI GDGCHC 1 cut(s) 447
SetI ASST 6 cut(s) 92, 125, 198, 293, 468, 508
SfcI CTRYAG 2 cut(s) 69, 539
SmiMI CAYNNNNRTG 1 cut(s) 316
Sse9I AATT 2 cut(s) 96, 270
SspMI CTAG 1 cut(s) 102
StyD4I CCNGG 3 cut(s) 89, 290, 517
TaaI ACNGT 2 cut(s) 128, 365
TaqI TCGA 2 cut(s) 397, 485
TasI AATT 2 cut(s) 96, 270
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TscAI CASTG 1 cut(s) 408
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 2 cut(s) 68, 431
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 3 cut(s) 271, 295, 427
TspRI CASTG 1 cut(s) 408
XapI RAATTY 1 cut(s) 96
XbaI TCTAGA 1 cut(s) 101
XmiI GTMKAC 1 cut(s) 369
XspI CTAG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.