FvH4_3g04720

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014505
Physical Location & Seq
Forward (+)
2699090 .. 2701388
2299 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04720.t1

Sequence Viewer

Length: 546 bp
ATGGCTTCAAGGCTGGTCTTGGGTGTCACTTTTGTGCTTGATCTGATTGCTTTTGCTCTTGCTGTGGCTGCTGAGCAGAGGAGGTCTACTTCTAAGATTCAGAAAGATGGAAACTCCAGCTACTGTGTGTATGACTCCGACATTGCCACCGGCTTAGGTGTTGGGGCATTTCTGTTCCTGTTGACAAGTCAGGCTCTGTTAATGATTGTAAGTCGATGCTTGTGCTGTGGAAAAGCATTGAGACCAAGTGGTTCTAGGTCATGGGCAATCGCGCTATTCATTACCAGCTGGGTATTTTTCTTCATTGCTGAGGCGTGCTTACTTGCTGGTTCAGTGAGAAATGCCTACCACACCAAGTACAGAACTAAGCTTTCTACTGAGAAGCTTTCATGCGAGACATTGAGGAAGGGGATCTTCGGGGCTGGCGCCGCCTTTGTGGTCTTCACAGGCATAGTATCTGAGTTTTACTATACTAGTTACTCCAAGGCTAATGATGCAGAGGCTCACTATGCCAGAGACAATGGAGTGAGGATGCAGAACCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

182

Amino Acids

19.76

Weight (kDa)

9.22

Isoelectric Point (pI)

34.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 56 - 150 1.7e-30 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61065
fragaria_vesca FvH4_3g04720
prunus_persica Prupe.4G042400_v2.0.a1 Prupe.4G042400_v2.0.a1
pyrus_communis pycom05g29480
rosa_chinensis RchiOBHm_Chr5g0007741
rosa_laevigata RLG00000031483
rosa_multiflora Rmu_sc0005098.1_g000021
rosa_roxburghii Rroxscaffold_1G00068670
rosa_rugosa Rorug04G0429800 Rorug04G0429900 Rorug04G0430000
rosa_samantha Rh5AG058300 Rh5BG055100 Rh5CG064800 Rh5DG054100
rosa_wichuraiana Rw5G006140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 425
AccI GTMKAC 1 cut(s) 86
AccII CGCG 1 cut(s) 272
AciI CCGC 1 cut(s) 429
AclWI GGATC 1 cut(s) 419
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 1 cut(s) 359
AgsI TTSAA 1 cut(s) 9
AhlI ACTAGT 1 cut(s) 473
AleI CACNNNNGTG 1 cut(s) 32
AluBI AGCT 4 cut(s) 120, 288, 370, 385
AluI AGCT 4 cut(s) 120, 288, 370, 385
Alw26I GTCTC 3 cut(s) 235, 389, 510
AlwI GGATC 1 cut(s) 419
AlwNI CAGNNNCTG 2 cut(s) 123, 196
ApeKI GCWGC 1 cut(s) 68
AspLEI GCGC 2 cut(s) 274, 428
BanI GGYRCC 1 cut(s) 425
BbsI GAAGAC 1 cut(s) 433
BbvCI CCTCAGC 1 cut(s) 309
BbvI GCAGC 1 cut(s) 55
BccI CCATC 1 cut(s) 101
BcgI CGANNNNNNTGC 2 cut(s) 204, 238
BcoDI GTCTC 3 cut(s) 235, 389, 510
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 2 cut(s) 255, 474
BfoI RGCGCY 1 cut(s) 429
BisI GCNGC 2 cut(s) 69, 429
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 2 cut(s) 70, 430
BmiI GGNNCC 1 cut(s) 427
BmsI GCATC 3 cut(s) 206, 484, 522
BpiI GAAGAC 1 cut(s) 433
BpmI CTGGAG 1 cut(s) 100
Bpu10I CCTNAGC 2 cut(s) 154, 309
Bpu1102I GCTNAGC 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 426
BsaI GGTCTC 1 cut(s) 235
BsaJI CCNNGG 1 cut(s) 483
Bse118I RCCGGY 1 cut(s) 149
Bse3DI GCAATG 2 cut(s) 141, 303
BseDI CCNNGG 1 cut(s) 483
BseGI GGATG 1 cut(s) 537
BseMI GCAATG 2 cut(s) 141, 303
BseMII CTCAG 4 cut(s) 63, 300, 369, 450
BseRI GAGGAG 1 cut(s) 94
BseXI GCAGC 1 cut(s) 55
BseYI CCCAGC 1 cut(s) 288
Bsh1236I CGCG 1 cut(s) 272
BshNI GGYRCC 1 cut(s) 425
BsiSI CCGG 1 cut(s) 150
BsmAI GTCTC 3 cut(s) 235, 389, 510
Bso31I GGTCTC 1 cut(s) 235
Bsp143I GATC 2 cut(s) 40, 411
Bsp1720I GCTNAGC 1 cut(s) 72
BspACI CCGC 1 cut(s) 429
BspCNI CTCAG 4 cut(s) 64, 301, 370, 451
BspFNI CGCG 1 cut(s) 272
BspLI GGNNCC 1 cut(s) 427
BspPI GGATC 1 cut(s) 419
BspT107I GGYRCC 1 cut(s) 425
BspTNI GGTCTC 1 cut(s) 235
BsrDI GCAATG 2 cut(s) 141, 303
BsrFI RCCGGY 1 cut(s) 149
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 483
BssMI GATC 2 cut(s) 40, 411
BssNI GRCGYC 1 cut(s) 426
BssT1I CCWWGG 1 cut(s) 483
Bst4CI ACNGT 1 cut(s) 125
BstACI GRCGYC 1 cut(s) 426
BstC8I GCNNGC 2 cut(s) 316, 424
BstDEI CTNAG 7 cut(s) 72, 93, 154, 309, 366, 378, 459
BstF5I GGATG 1 cut(s) 537
BstFNI CGCG 1 cut(s) 272
BstH2I RGCGCY 1 cut(s) 429
BstHHI GCGC 2 cut(s) 274, 428
BstKTI GATC 2 cut(s) 43, 414
BstMAI GTCTC 3 cut(s) 235, 389, 510
BstMBI GATC 2 cut(s) 40, 411
BstMWI GCNNNNNNNGC 4 cut(s) 68, 428, 494, 509
BstUI CGCG 1 cut(s) 272
BstV1I GCAGC 1 cut(s) 55
BstV2I GAAGAC 1 cut(s) 433
BstX2I RGATCY 1 cut(s) 411
BstYI RGATCY 1 cut(s) 411
BtsCI GGATG 1 cut(s) 537
BtsIMutI CAGTG 1 cut(s) 339
Cac8I GCNNGC 2 cut(s) 316, 424
CaiI CAGNNNCTG 2 cut(s) 123, 196
CfoI GCGC 2 cut(s) 274, 428
Cfr10I RCCGGY 1 cut(s) 149
Csp6I GTAC 1 cut(s) 358
CviAII CATG 2 cut(s) 261, 390
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 7 cut(s) 72, 93, 154, 309, 366, 378, 459
DinI GGCGCC 1 cut(s) 427
DpnI GATC 2 cut(s) 42, 413
DpnII GATC 2 cut(s) 40, 411
Eco130I CCWWGG 1 cut(s) 483
Eco31I GGTCTC 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 483
EgeI GGCGCC 1 cut(s) 427
EheI GGCGCC 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 483
FaeI CATG 2 cut(s) 264, 393
FaiI YATR 6 cut(s) 132, 262, 391, 452, 471, 510
FalI AAGNNNNNCTT 2 cut(s) 398, 430
FatI CATG 2 cut(s) 260, 389
FblI GTMKAC 1 cut(s) 86
Fnu4HI GCNGC 2 cut(s) 69, 429
Fsp4HI GCNGC 2 cut(s) 69, 429
FspBI CTAG 2 cut(s) 255, 474
GlaI GCGC 2 cut(s) 273, 427
GluI GCNGC 2 cut(s) 69, 429
GsaI CCCAGC 1 cut(s) 292
GsuI CTGGAG 1 cut(s) 100
HaeII RGCGCY 1 cut(s) 429
HapII CCGG 1 cut(s) 150
HhaI GCGC 2 cut(s) 274, 428
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 2 cut(s) 264, 393
Hin6I GCGC 2 cut(s) 272, 426
HinP1I GCGC 2 cut(s) 272, 426
HincII GTYRAC 1 cut(s) 183
HindII GTYRAC 1 cut(s) 183
HindIII AAGCTT 2 cut(s) 368, 383
HinfI GANTC 2 cut(s) 97, 134
HpaII CCGG 1 cut(s) 150
Hpy166II GTNNAC 2 cut(s) 87, 183
Hpy188I TCNGA 4 cut(s) 45, 102, 139, 460
Hpy8I GTNNAC 2 cut(s) 87, 183
HpyAV CCTTC 1 cut(s) 400
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4V TGCA 2 cut(s) 497, 535
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 428, 494, 509
HpyF3I CTNAG 7 cut(s) 72, 93, 154, 309, 366, 378, 459
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 2 cut(s) 264, 393
HspAI GCGC 2 cut(s) 272, 426
KasI GGCGCC 1 cut(s) 425
Kzo9I GATC 2 cut(s) 40, 411
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 3 cut(s) 206, 484, 522
MaeI CTAG 2 cut(s) 255, 474
MaeIII GTNAC 2 cut(s) 25, 476
MalI GATC 2 cut(s) 42, 413
MboI GATC 2 cut(s) 40, 411
MboII GAAGA 3 cut(s) 292, 406, 433
MflI RGATCY 1 cut(s) 411
Mly113I GGCGCC 1 cut(s) 426
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 6 cut(s) 72, 75, 304, 396, 493, 522
MseI TTAA 1 cut(s) 200
MslI CAYNNNNRTG 1 cut(s) 32
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 1 cut(s) 150
MvnI CGCG 1 cut(s) 272
MwoI GCNNNNNNNGC 4 cut(s) 68, 428, 494, 509
NarI GGCGCC 1 cut(s) 426
NdeII GATC 2 cut(s) 40, 411
NlaIII CATG 2 cut(s) 264, 393
NlaIV GGNNCC 1 cut(s) 427
NmuCI GTSAC 1 cut(s) 25
OliI CACNNNNGTG 1 cut(s) 32
PfeI GAWTC 1 cut(s) 97
PkrI GCNGC 2 cut(s) 70, 430
PleI GAGTC 1 cut(s) 128
PluTI GGCGCC 1 cut(s) 429
PpsI GAGTC 1 cut(s) 128
PspFI CCCAGC 1 cut(s) 288
PspN4I GGNNCC 1 cut(s) 427
PstNI CAGNNNCTG 2 cut(s) 123, 196
PsuI RGATCY 1 cut(s) 411
PvuII CAGCTG 1 cut(s) 288
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
RseI CAYNNNNRTG 1 cut(s) 32
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 2 cut(s) 69, 429
Sau3AI GATC 2 cut(s) 40, 411
SchI GAGTC 1 cut(s) 128
SetI ASST 8 cut(s) 86, 122, 160, 260, 290, 372, 387, 543
SfaNI GCATC 3 cut(s) 206, 484, 522
SfoI GGCGCC 1 cut(s) 427
SmiMI CAYNNNNRTG 1 cut(s) 32
SpeI ACTAGT 1 cut(s) 473
SsiI CCGC 1 cut(s) 429
SspDI GGCGCC 1 cut(s) 425
SspMI CTAG 2 cut(s) 255, 474
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 1 cut(s) 125
TaqI TCGA 1 cut(s) 214
TatI WGTACW 1 cut(s) 357
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 1 cut(s) 97
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TscAI CASTG 1 cut(s) 339
TseFI GTSAC 1 cut(s) 25
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 25
TspDTI ATGAA 3 cut(s) 268, 292, 378
TspRI CASTG 1 cut(s) 339
XmiI GTMKAC 1 cut(s) 86
XspI CTAG 2 cut(s) 255, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.