Rorug04G0429900

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014505
Physical Location & Seq
Reverse (-)
58741240 .. 58742416
1177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0429900.1

Sequence Viewer

Length: 585 bp
ATGGCCACCGAGGAGTCCAACGAGCAACCGCCACCGTCGCCGGCACCGCCTAATCCGGCTCCGGCGCTCCCTGAGTACCCAAAGATGATTATGGCGGCGATTGAGGCGCTTAATGAGAGCGAGGGGTCGAACAAGACCTCAATCGCGCAGCAAATCGAGTCCACCTACGGCGATCTGCCGCCGGCTCACGCCACGCTTCTGGCGCACCACCTCAACAAGATGAAGCAGAGCGGCGAGCTCGTTTTCGTCAAGAACAACTACATGAAGCCCGACCCCAACGCGCCGCCCAAGCGGGGCCGTGGCCGCCCGCCCAAGCCCAAGGTCCCGGCGCCGGAAGGAGCCGCCGTGGTCTCCTCGCCTCCGAGGCCACGTGGCAGACCTCCCAAGCCTAGGGATCCGTTTTTCCCGCTTCCAATTGCCAAGAGCTCGACATCCACCGGGTCCGGCAGGCCACGCGGTCGCCCTCCGAAGAAGGCCAAGACAAGTCCGACCTCGTCTCCGGCTTCGGCTCCGGCGAGCGGTGCCCCTAGAGGTAGAGGCAGGCCACCGAAAGTCAAGCCCTCGGTTGCTCCGGTGGGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

194

Amino Acids

20.33

Weight (kDa)

10.41

Isoelectric Point (pI)

80.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Linker_histone PF00538 24 - 85 5.2e-09 linker histone H1 and H5 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61065
fragaria_vesca FvH4_3g04720
prunus_persica Prupe.4G042400_v2.0.a1 Prupe.4G042400_v2.0.a1
pyrus_communis pycom05g29480
rosa_chinensis RchiOBHm_Chr5g0007741
rosa_laevigata RLG00000031483
rosa_multiflora Rmu_sc0005098.1_g000021
rosa_roxburghii Rroxscaffold_1G00068670
rosa_rugosa Rorug04G0429800 Rorug04G0429900 Rorug04G0430000
rosa_samantha Rh5AG058300 Rh5BG055100 Rh5CG064800 Rh5DG054100
rosa_wichuraiana Rw5G006140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 43, 328, 521
AccBSI CCGCTC 2 cut(s) 231, 519
AccII CGCG 3 cut(s) 146, 281, 456
AclWI GGATC 2 cut(s) 389, 402
AcoI YGGCCR 2 cut(s) 3, 301
AcvI CACGTG 1 cut(s) 371
AcyI GRCGYC 1 cut(s) 329
AfaI GTAC 1 cut(s) 77
AfiI CCNNNNNNNGG 4 cut(s) 293, 331, 390, 518
AluBI AGCT 2 cut(s) 238, 426
AluI AGCT 2 cut(s) 238, 426
Alw21I GWGCWC 2 cut(s) 240, 428
Alw26I GTCTC 2 cut(s) 355, 501
AlwI GGATC 2 cut(s) 389, 402
AoxI GGCC 7 cut(s) 3, 295, 301, 365, 449, 474, 542
ApeKI GCWGC 1 cut(s) 148
AspA2I CCTAGG 1 cut(s) 389
AspLEI GCGC 6 cut(s) 67, 109, 148, 205, 283, 331
AspS9I GGNCC 3 cut(s) 295, 322, 441
AsuC2I CCSGG 2 cut(s) 326, 439
AvaII GGWCC 2 cut(s) 322, 441
AvrII CCTAGG 1 cut(s) 389
BaeGI GKGCMC 1 cut(s) 526
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 394
BanI GGYRCC 3 cut(s) 43, 328, 521
BanII GRGCYC 2 cut(s) 240, 428
BbrPI CACGTG 1 cut(s) 371
Bbv12I GWGCWC 2 cut(s) 240, 428
BbvI GCAGC 1 cut(s) 160
BceAI ACGGC 3 cut(s) 184, 282, 329
BcnI CCSGG 2 cut(s) 326, 439
BcoDI GTCTC 2 cut(s) 355, 501
BfaI CTAG 2 cut(s) 390, 528
BfoI RGCGCY 3 cut(s) 68, 110, 332
BglI GCCNNNNNGGC 1 cut(s) 364
BisI GCNGC 7 cut(s) 96, 149, 179, 232, 284, 304, 342
BlnI CCTAGG 1 cut(s) 389
BlsI GCNGC 7 cut(s) 97, 150, 180, 233, 285, 305, 343
Bme1390I CCNGG 2 cut(s) 326, 439
Bme18I GGWCC 2 cut(s) 322, 441
BmgT120I GGNCC 3 cut(s) 295, 322, 441
BmrFI CCNGG 2 cut(s) 326, 439
BpuMI CCSGG 2 cut(s) 326, 439
BsaAI YACGTR 1 cut(s) 371
BsaHI GRCGYC 1 cut(s) 329
BsaI GGTCTC 1 cut(s) 355
BsaJI CCNNGG 7 cut(s) 9, 298, 318, 345, 362, 389, 561
BsaWI WCCGGW 1 cut(s) 571
BsaXI ACNNNNNCTCC 4 cut(s) 330, 360, 481, 511
Bsc4I CCNNNNNNNGG 4 cut(s) 293, 331, 390, 518
Bse118I RCCGGY 2 cut(s) 40, 181
BseDI CCNNGG 7 cut(s) 9, 298, 318, 345, 362, 389, 561
BseGI GGATG 1 cut(s) 431
BseLI CCNNNNNNNGG 4 cut(s) 293, 331, 390, 518
BseMII CTCAG 1 cut(s) 63
BseRI GAGGAG 2 cut(s) 26, 343
BseSI GKGCMC 1 cut(s) 526
BseXI GCAGC 1 cut(s) 160
Bsh1236I CGCG 3 cut(s) 146, 281, 456
Bsh1285I CGRYCG 1 cut(s) 460
BshFI GGCC 7 cut(s) 5, 297, 303, 367, 451, 476, 544
BshNI GGYRCC 3 cut(s) 43, 328, 521
BsiEI CGRYCG 1 cut(s) 460
BsiHKAI GWGCWC 2 cut(s) 240, 428
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 4 cut(s) 293, 331, 390, 518
BsmAI GTCTC 2 cut(s) 355, 501
BsmBI CGTCTC 1 cut(s) 501
BsmFI GGGAC 1 cut(s) 308
BsnI GGCC 7 cut(s) 5, 297, 303, 367, 451, 476, 544
Bso31I GGTCTC 1 cut(s) 355
Bsp1286I GDGCHC 3 cut(s) 240, 428, 526
Bsp143I GATC 2 cut(s) 172, 394
BspANI GGCC 7 cut(s) 5, 297, 303, 367, 451, 476, 544
BspCNI CTCAG 1 cut(s) 64
BspFNI CGCG 3 cut(s) 146, 281, 456
BspPI GGATC 2 cut(s) 389, 402
BspT107I GGYRCC 3 cut(s) 43, 328, 521
BspTNI GGTCTC 1 cut(s) 355
BsrBI CCGCTC 2 cut(s) 231, 519
BsrFI RCCGGY 2 cut(s) 40, 181
BssAI RCCGGY 2 cut(s) 40, 181
BssECI CCNNGG 7 cut(s) 9, 298, 318, 345, 362, 389, 561
BssMI GATC 2 cut(s) 172, 394
BssNI GRCGYC 1 cut(s) 329
BssT1I CCWWGG 2 cut(s) 318, 389
Bst4CI ACNGT 1 cut(s) 36
BstACI GRCGYC 1 cut(s) 329
BstBAI YACGTR 1 cut(s) 371
BstC8I GCNNGC 7 cut(s) 42, 183, 236, 308, 449, 517, 542
BstDEI CTNAG 1 cut(s) 72
BstDSI CCRYGG 2 cut(s) 298, 345
BstF5I GGATG 1 cut(s) 431
BstFNI CGCG 3 cut(s) 146, 281, 456
BstH2I RGCGCY 3 cut(s) 68, 110, 332
BstHHI GCGC 6 cut(s) 67, 109, 148, 205, 283, 331
BstKTI GATC 2 cut(s) 175, 397
BstMAI GTCTC 2 cut(s) 355, 501
BstMBI GATC 2 cut(s) 172, 394
BstMCI CGRYCG 1 cut(s) 460
BstMWI GCNNNNNNNGC 9 cut(s) 37, 46, 104, 202, 289, 303, 364, 453, 521
BstSCI CCNGG 2 cut(s) 324, 437
BstSLI GKGCMC 1 cut(s) 526
BstUI CGCG 3 cut(s) 146, 281, 456
BstV1I GCAGC 1 cut(s) 160
BstX2I RGATCY 1 cut(s) 394
BstXI CCANNNNNNTGG 1 cut(s) 199
BstYI RGATCY 1 cut(s) 394
BsuRI GGCC 7 cut(s) 5, 297, 303, 367, 451, 476, 544
BtgI CCRYGG 2 cut(s) 298, 345
BtsCI GGATG 1 cut(s) 431
Cac8I GCNNGC 7 cut(s) 42, 183, 236, 308, 449, 517, 542
CfoI GCGC 6 cut(s) 67, 109, 148, 205, 283, 331
Cfr10I RCCGGY 2 cut(s) 40, 181
Cfr13I GGNCC 3 cut(s) 295, 322, 441
Csp6I GTAC 1 cut(s) 76
CviAII CATG 1 cut(s) 262
CviQI GTAC 1 cut(s) 76
DdeI CTNAG 1 cut(s) 72
DinI GGCGCC 1 cut(s) 330
DpnI GATC 2 cut(s) 174, 396
DpnII GATC 2 cut(s) 172, 394
EaeI YGGCCR 2 cut(s) 3, 301
Ecl136II GAGCTC 2 cut(s) 238, 426
Eco130I CCWWGG 2 cut(s) 318, 389
Eco24I GRGCYC 2 cut(s) 240, 428
Eco31I GGTCTC 1 cut(s) 355
Eco47I GGWCC 2 cut(s) 322, 441
Eco53kI GAGCTC 2 cut(s) 238, 426
Eco72I CACGTG 1 cut(s) 371
EcoICRI GAGCTC 2 cut(s) 238, 426
EcoO109I RGGNCCY 1 cut(s) 322
EcoT14I CCWWGG 2 cut(s) 318, 389
EcoT38I GRGCYC 2 cut(s) 240, 428
EgeI GGCGCC 1 cut(s) 330
EheI GGCGCC 1 cut(s) 330
ErhI CCWWGG 2 cut(s) 318, 389
Esp3I CGTCTC 1 cut(s) 501
FaeI CATG 1 cut(s) 265
FaiI YATR 2 cut(s) 92, 263
FaqI GGGAC 1 cut(s) 308
FatI CATG 1 cut(s) 261
FauI CCCGC 3 cut(s) 285, 315, 414
Fnu4HI GCNGC 7 cut(s) 96, 149, 179, 232, 284, 304, 342
FokI GGATG 1 cut(s) 418
FriOI GRGCYC 2 cut(s) 240, 428
Fsp4HI GCNGC 7 cut(s) 96, 149, 179, 232, 284, 304, 342
FspBI CTAG 2 cut(s) 390, 528
GlaI GCGC 6 cut(s) 66, 108, 147, 204, 282, 330
GluI GCNGC 7 cut(s) 96, 149, 179, 232, 284, 304, 342
HaeII RGCGCY 3 cut(s) 68, 110, 332
HaeIII GGCC 7 cut(s) 5, 297, 303, 367, 451, 476, 544
HhaI GCGC 6 cut(s) 67, 109, 148, 205, 283, 331
Hin1I GRCGYC 1 cut(s) 329
Hin1II CATG 1 cut(s) 265
Hin6I GCGC 6 cut(s) 65, 107, 146, 203, 281, 329
HinP1I GCGC 6 cut(s) 65, 107, 146, 203, 281, 329
HinfI GANTC 2 cut(s) 14, 158
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 3 cut(s) 363, 468, 489
Hpy188III TCNNGA 1 cut(s) 250
Hpy8I GTNNAC 1 cut(s) 162
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 2 cut(s) 329, 466
HpyCH4III ACNGT 1 cut(s) 36
HpyCH4IV ACGT 1 cut(s) 370
HpyF10VI GCNNNNNNNGC 9 cut(s) 37, 46, 104, 202, 289, 303, 364, 453, 521
HpyF3I CTNAG 1 cut(s) 72
HpySE526I ACGT 1 cut(s) 370
Hsp92I GRCGYC 1 cut(s) 329
Hsp92II CATG 1 cut(s) 265
HspAI GCGC 6 cut(s) 65, 107, 146, 203, 281, 329
KasI GGCGCC 1 cut(s) 328
KroI GCCGGC 2 cut(s) 40, 181
KroNI GCCGGC 2 cut(s) 42, 183
Kzo9I GATC 2 cut(s) 172, 394
LmnI GCTCC 5 cut(s) 64, 72, 338, 514, 574
Lsp1109I GCAGC 1 cut(s) 160
MaeI CTAG 2 cut(s) 390, 528
MaeII ACGT 1 cut(s) 370
MalI GATC 2 cut(s) 174, 396
MbiI CCGCTC 2 cut(s) 231, 519
MboI GATC 2 cut(s) 172, 394
MboII GAAGA 1 cut(s) 481
MfeI CAATTG 1 cut(s) 414
MflI RGATCY 1 cut(s) 394
MhlI GDGCHC 3 cut(s) 240, 428, 526
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 414
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 329
MlyI GAGTC 2 cut(s) 23, 167
MmeI TCCRAC 2 cut(s) 42, 512
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 2 cut(s) 40, 181
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 111
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 2 cut(s) 326, 439
MunI CAATTG 1 cut(s) 414
MvnI CGCG 3 cut(s) 146, 281, 456
MwoI GCNNNNNNNGC 9 cut(s) 37, 46, 104, 202, 289, 303, 364, 453, 521
NaeI GCCGGC 2 cut(s) 42, 183
NarI GGCGCC 1 cut(s) 329
NciI CCSGG 2 cut(s) 326, 439
NdeII GATC 2 cut(s) 172, 394
NgoMIV GCCGGC 2 cut(s) 40, 181
NlaIII CATG 1 cut(s) 265
PcsI WCGNNNNNNNCGW 1 cut(s) 512
PdiI GCCGGC 2 cut(s) 42, 183
PflFI GACNNNGTC 1 cut(s) 493
PkrI GCNGC 7 cut(s) 97, 150, 180, 233, 285, 305, 343
PleI GAGTC 2 cut(s) 22, 166
PluTI GGCGCC 1 cut(s) 332
PmaCI CACGTG 1 cut(s) 371
PmlI CACGTG 1 cut(s) 371
PpsI GAGTC 2 cut(s) 22, 166
Ppu21I YACGTR 1 cut(s) 371
PpuMI RGGWCCY 1 cut(s) 322
Psp124BI GAGCTC 2 cut(s) 240, 428
Psp5II RGGWCCY 1 cut(s) 322
PspCI CACGTG 1 cut(s) 371
PspPI GGNCC 3 cut(s) 295, 322, 441
PspPPI RGGWCCY 1 cut(s) 322
PsuI RGATCY 1 cut(s) 394
PsyI GACNNNGTC 1 cut(s) 493
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
SacI GAGCTC 2 cut(s) 240, 428
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 7 cut(s) 96, 149, 179, 232, 284, 304, 342
Sau3AI GATC 2 cut(s) 172, 394
Sau96I GGNCC 3 cut(s) 295, 322, 441
SchI GAGTC 2 cut(s) 23, 167
ScrFI CCNGG 2 cut(s) 326, 439
SduI GDGCHC 3 cut(s) 240, 428, 526
SfoI GGCGCC 1 cut(s) 330
SinI GGWCC 2 cut(s) 322, 441
Sse9I AATT 1 cut(s) 414
SspDI GGCGCC 1 cut(s) 328
SspMI CTAG 2 cut(s) 390, 528
SstI GAGCTC 2 cut(s) 240, 428
StyD4I CCNGG 2 cut(s) 324, 437
StyI CCWWGG 2 cut(s) 318, 389
TaaI ACNGT 1 cut(s) 36
TaiI ACGT 1 cut(s) 373
TaqI TCGA 3 cut(s) 128, 156, 428
TasI AATT 1 cut(s) 414
TauI GCSGC 6 cut(s) 98, 181, 234, 286, 306, 344
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TseI GCWGC 1 cut(s) 148
TspDTI ATGAA 2 cut(s) 236, 278
TspGWI ACGGA 1 cut(s) 387
Tth111I GACNNNGTC 1 cut(s) 493
VpaK11BI GGWCC 2 cut(s) 322, 441
XmaJI CCTAGG 1 cut(s) 389
XspI CTAG 2 cut(s) 390, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.