Rorug04G0430000

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014505
Physical Location & Seq
Reverse (-)
58747057 .. 58747619
563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0430000.1

Sequence Viewer

Length: 456 bp
ATGGCTTCGAACAAACTTGTGACCTTAATCGCCGCTGTTCTGATTCTTCTCCCTGCGATGGCCATGGCGAAAGATATCGTCGTCGGAGACGATAGCGGGTGGAAGCTGAATTTCGATTACCAAGCTTGGGCCAAGGACAAAGTATTTCAAGTTGGTGATACACTAGTGTTCAAGTACAAACCTCCTACTCACAATGTGTACAAGGTGGACGGAGCTGGGTTCAAGGATTGCACTAAACCGGCAGCAAACGAAGCATTGACTAGTGGAAATGACAAAGTAGAACTGAAAGCCGCCGGAAAGAAATGGTACATTTGCGGTGTTTCCGATCACTGTGATCAGGGGATGAAGCTTACCCTTAACGTTACGGAGAAGTCTTCTGCGGCTCGTGGGATCATCTTCCCCCGAAGCCAAGTCTTCGGGGTCGCCATCTTTGCCATCGTAGCAATTGTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

151

Amino Acids

16.34

Weight (kDa)

8.94

Isoelectric Point (pI)

15.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 34 - 115 1.6e-26 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014505)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61065
fragaria_vesca FvH4_3g04720
prunus_persica Prupe.4G042400_v2.0.a1 Prupe.4G042400_v2.0.a1
pyrus_communis pycom05g29480
rosa_chinensis RchiOBHm_Chr5g0007741
rosa_laevigata RLG00000031483
rosa_multiflora Rmu_sc0005098.1_g000021
rosa_roxburghii Rroxscaffold_1G00068670
rosa_rugosa Rorug04G0429800 Rorug04G0429900 Rorug04G0430000
rosa_samantha Rh5AG058300 Rh5BG055100 Rh5CG064800 Rh5DG054100
rosa_wichuraiana Rw5G006140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 33, 96, 291, 315, 380
AclI AACGTT 1 cut(s) 360
AclWI GGATC 1 cut(s) 398
AcoI YGGCCR 1 cut(s) 60
AcsI RAATTY 1 cut(s) 109
AdeI CACNNNGTG 1 cut(s) 196
AfaI GTAC 3 cut(s) 176, 200, 308
AfiI CCNNNNNNNGG 2 cut(s) 58, 127
AgsI TTSAA 3 cut(s) 149, 172, 223
AhlI ACTAGT 2 cut(s) 163, 260
AluBI AGCT 4 cut(s) 106, 125, 215, 349
AluI AGCT 4 cut(s) 106, 125, 215, 349
Alw26I GTCTC 1 cut(s) 81
AlwI GGATC 1 cut(s) 398
AoxI GGCC 2 cut(s) 60, 129
ApeKI GCWGC 1 cut(s) 242
ApoI RAATTY 1 cut(s) 109
AspS9I GGNCC 1 cut(s) 129
AsuHPI GGTGA 1 cut(s) 167
AsuII TTCGAA 1 cut(s) 8
BaeI ACNNNNGTAYC 2 cut(s) 150, 183
BalI TGGCCA 1 cut(s) 62
BauI CACGAG 1 cut(s) 384
BbsI GAAGAC 2 cut(s) 366, 406
BbvI GCAGC 1 cut(s) 254
BccI CCATC 3 cut(s) 52, 434, 443
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 1 cut(s) 81
BcuI ACTAGT 2 cut(s) 163, 260
BfaI CTAG 2 cut(s) 164, 261
BisI GCNGC 4 cut(s) 33, 243, 291, 381
BlsI GCNGC 4 cut(s) 34, 244, 292, 382
BmgT120I GGNCC 1 cut(s) 129
BpiI GAAGAC 2 cut(s) 366, 406
Bpu14I TTCGAA 1 cut(s) 8
BsaJI CCNNGG 2 cut(s) 63, 132
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 127
Bse118I RCCGGY 1 cut(s) 238
BseDI CCNNGG 2 cut(s) 63, 132
BseGI GGATG 1 cut(s) 348
BseLI CCNNNNNNNGG 2 cut(s) 58, 127
BseXI GCAGC 1 cut(s) 254
BseYI CCCAGC 1 cut(s) 215
BshFI GGCC 2 cut(s) 62, 131
BsiSI CCGG 2 cut(s) 239, 294
BslI CCNNNNNNNGG 2 cut(s) 58, 127
BsmAI GTCTC 1 cut(s) 81
BsmBI CGTCTC 1 cut(s) 81
BsnI GGCC 2 cut(s) 62, 131
Bsp119I TTCGAA 1 cut(s) 8
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 3 cut(s) 325, 334, 390
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 5 cut(s) 33, 96, 291, 315, 380
BspANI GGCC 2 cut(s) 62, 131
BspPI GGATC 1 cut(s) 398
BspT104I TTCGAA 1 cut(s) 8
BsrFI RCCGGY 1 cut(s) 238
BsrGI TGTACA 1 cut(s) 198
BssAI RCCGGY 1 cut(s) 238
BssECI CCNNGG 2 cut(s) 63, 132
BssMI GATC 3 cut(s) 325, 334, 390
BssSI CACGAG 1 cut(s) 384
BssT1I CCWWGG 2 cut(s) 63, 132
Bst2BI CACGAG 1 cut(s) 384
Bst4CI ACNGT 1 cut(s) 332
BstAUI TGTACA 1 cut(s) 198
BstBI TTCGAA 1 cut(s) 8
BstDSI CCRYGG 1 cut(s) 63
BstF5I GGATG 1 cut(s) 348
BstKTI GATC 3 cut(s) 328, 337, 393
BstMAI GTCTC 1 cut(s) 81
BstMBI GATC 3 cut(s) 325, 334, 390
BstMWI GCNNNNNNNGC 3 cut(s) 251, 431, 440
BstV1I GCAGC 1 cut(s) 254
BstV2I GAAGAC 2 cut(s) 366, 406
BsuRI GGCC 2 cut(s) 62, 131
BtgI CCRYGG 1 cut(s) 63
BtgZI GCGATG 1 cut(s) 71
BtsCI GGATG 1 cut(s) 348
BtsIMutI CAGTG 1 cut(s) 328
Cfr10I RCCGGY 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 129
Csp6I GTAC 3 cut(s) 175, 199, 307
CviAII CATG 1 cut(s) 64
CviQI GTAC 3 cut(s) 175, 199, 307
DpnI GATC 3 cut(s) 327, 336, 392
DpnII GATC 3 cut(s) 325, 334, 390
DraIII CACNNNGTG 1 cut(s) 196
EaeI YGGCCR 1 cut(s) 60
Eco130I CCWWGG 2 cut(s) 63, 132
Eco32I GATATC 1 cut(s) 76
EcoRV GATATC 1 cut(s) 76
EcoT14I CCWWGG 2 cut(s) 63, 132
ErhI CCWWGG 2 cut(s) 63, 132
Esp3I CGTCTC 1 cut(s) 81
FaeI CATG 1 cut(s) 67
FaiI YATR 1 cut(s) 65
FatI CATG 1 cut(s) 63
FauI CCCGC 1 cut(s) 89
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 4 cut(s) 33, 243, 291, 381
FokI GGATG 1 cut(s) 355
Fsp4HI GCNGC 4 cut(s) 33, 243, 291, 381
FspBI CTAG 2 cut(s) 164, 261
GluI GCNGC 4 cut(s) 33, 243, 291, 381
GsaI CCCAGC 1 cut(s) 219
HaeIII GGCC 2 cut(s) 62, 131
HapII CCGG 2 cut(s) 239, 294
Hin1II CATG 1 cut(s) 67
HindIII AAGCTT 2 cut(s) 123, 347
HinfI GANTC 1 cut(s) 43
HpaII CCGG 2 cut(s) 239, 294
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 2 cut(s) 199, 208
Hpy188I TCNGA 3 cut(s) 42, 86, 325
Hpy8I GTNNAC 2 cut(s) 199, 208
Hpy99I CGWCG 2 cut(s) 83, 86
HpyCH4III ACNGT 1 cut(s) 332
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 1 cut(s) 231
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 431, 440
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 1 cut(s) 67
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 3 cut(s) 325, 334, 390
LmnI GCTCC 1 cut(s) 212
LpnPI CCDG 5 cut(s) 66, 201, 252, 307, 323
Lsp1109I GCAGC 1 cut(s) 254
MaeI CTAG 2 cut(s) 164, 261
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 2 cut(s) 19, 361
MalI GATC 3 cut(s) 327, 336, 392
MboI GATC 3 cut(s) 325, 334, 390
MboII GAAGA 4 cut(s) 38, 366, 388, 406
MfeI CAATTG 1 cut(s) 444
MlsI TGGCCA 1 cut(s) 62
MluCI AATT 2 cut(s) 109, 444
MluNI TGGCCA 1 cut(s) 62
MmeI TCCRAC 1 cut(s) 64
MnlI CCTC 1 cut(s) 192
Mox20I TGGCCA 1 cut(s) 62
MscI TGGCCA 1 cut(s) 62
MseI TTAA 2 cut(s) 26, 357
Msp20I TGGCCA 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 2 cut(s) 239, 294
MunI CAATTG 1 cut(s) 444
MwoI GCNNNNNNNGC 3 cut(s) 251, 431, 440
NcoI CCATGG 1 cut(s) 63
NdeII GATC 3 cut(s) 325, 334, 390
NlaIII CATG 1 cut(s) 67
NmuCI GTSAC 1 cut(s) 19
NspV TTCGAA 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PfeI GAWTC 1 cut(s) 43
PkrI GCNGC 4 cut(s) 34, 244, 292, 382
Psp1406I AACGTT 1 cut(s) 360
PspFI CCCAGC 1 cut(s) 215
PspPI GGNCC 1 cut(s) 129
RsaI GTAC 3 cut(s) 176, 200, 308
RsaNI GTAC 3 cut(s) 175, 199, 307
SaqAI TTAA 2 cut(s) 26, 357
SatI GCNGC 4 cut(s) 33, 243, 291, 381
Sau3AI GATC 3 cut(s) 325, 334, 390
Sau96I GGNCC 1 cut(s) 129
SetI ASST 8 cut(s) 26, 108, 127, 184, 207, 217, 351, 363
SfuI TTCGAA 1 cut(s) 8
SpeI ACTAGT 2 cut(s) 163, 260
Sse9I AATT 2 cut(s) 109, 444
SsiI CCGC 5 cut(s) 33, 96, 291, 315, 380
SspMI CTAG 2 cut(s) 164, 261
StyI CCWWGG 2 cut(s) 63, 132
TaaI ACNGT 1 cut(s) 332
TaiI ACGT 1 cut(s) 363
TaqI TCGA 2 cut(s) 8, 114
TasI AATT 2 cut(s) 109, 444
TatI WGTACW 2 cut(s) 174, 198
TauI GCSGC 3 cut(s) 35, 293, 383
TfiI GAWTC 1 cut(s) 43
Tru1I TTAA 2 cut(s) 26, 357
Tru9I TTAA 2 cut(s) 26, 357
TscAI CASTG 1 cut(s) 335
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 1 cut(s) 242
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 1 cut(s) 359
TspGWI ACGGA 2 cut(s) 225, 380
TspRI CASTG 1 cut(s) 335
XapI RAATTY 1 cut(s) 109
XspI CTAG 2 cut(s) 164, 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.