AT1G64700

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0015004
Physical Location & Seq
Reverse (-)
24039706 .. 24040636
931 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G64700.1

Sequence Viewer

Length: 612 bp
ATGGGAAACTGTTTATTCGGAGGGTTAGGCGACGAAGAGGAAGACTTATTGATCAAAGTCATAAAATCCGACGGTGGAGTTCTTGAGTTCTACTCCCCCGTTACCGCGGGCTTTGTCTCCCACGGATTCTCAGGCCATGCTCTTTTCTCGGCTGTTGATCTCCTCTGGAAACCCCTGGCTCACGACCACCTCCTCGTGCCCGGTCAGTCCTACTATCTCTTTCCCAATATTGTCTCCGACGAGTTGAAGACTTTTGTGGGAAGCTGCCACGTGAGATCCAACAGCGAGTCCTTGTCTGCGATAACTCCGTACAGAATGTCTCTGGATTACAACCACAGGGTGTTGAAGCGATCATACACGGACGTTTTTTCAAGAAACAGCCACATAAGGACCCGACAAAAGGAGAAGAAAACGAGGAGGAGACGTACGAGCAGCAAAGGTGCAATATGGAAAGTGAATCTGATCATAAACACTGAAGAGCTGTTACAGATTCTGTCTGAAGATGGAAGAACAAATGAGCTAATAGAGAGTGTGAGAGCCGTAGCCAAGGGTGAAACATCGAGCATCACAAGTAGTTCGTCCGAGAATTTCTTGTCGGTGGTACAGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

203

Amino Acids

22.68

Weight (kDa)

7.82

Isoelectric Point (pI)

60.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 149 7.5e-16 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015004)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64700
fragaria_vesca FvH4_5g10680
malus_domestica MD06G1121500.v1.1 MD14G1138100.v1.1
prunus_persica Prupe.5G132000_v2.0.a1
pyrus_communis pycom14g11320
rosa_chinensis RchiOBHm_Chr7g0187471
rosa_laevigata RLG00000004778
rosa_multiflora Rmu_sc0001230.1_g000008
rosa_roxburghii Rroxscaffold_3G00267400
rosa_rugosa Rorug06G0481800
rosa_samantha Rh7AG086300 Rh7BG087900 Rh7CG087300 Rh7DG088900
rosa_wichuraiana Rw7G007620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 107
AciI CCGC 2 cut(s) 105, 107
AclWI GGATC 1 cut(s) 270
AcsI RAATTY 1 cut(s) 586
AcuI CTGAAG 2 cut(s) 495, 519
AcvI CACGTG 1 cut(s) 271
AdeI CACNNNGTG 1 cut(s) 340
AfaI GTAC 3 cut(s) 311, 427, 603
AfiI CCNNNNNNNGG 1 cut(s) 400
AgsI TTSAA 3 cut(s) 247, 346, 372
AjnI CCWGG 1 cut(s) 174
AluBI AGCT 3 cut(s) 264, 481, 520
AluI AGCT 3 cut(s) 264, 481, 520
Alw26I GTCTC 4 cut(s) 121, 238, 324, 415
AlwI GGATC 1 cut(s) 270
AlwNI CAGNNNCTG 1 cut(s) 493
AoxI GGCC 1 cut(s) 133
ApeKI GCWGC 2 cut(s) 264, 432
ApoI RAATTY 1 cut(s) 586
AspS9I GGNCC 1 cut(s) 390
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 1 cut(s) 563
AvaII GGWCC 1 cut(s) 390
BaeGI GKGCMC 1 cut(s) 201
BarI GAAGNNNNNNTAC 4 cut(s) 338, 370, 468, 500
BauI CACGAG 1 cut(s) 194
BbrPI CACGTG 1 cut(s) 271
BbsI GAAGAC 2 cut(s) 48, 254
BbvI GCAGC 2 cut(s) 251, 444
BccI CCATC 1 cut(s) 497
BceAI ACGGC 1 cut(s) 524
BciT130I CCWGG 1 cut(s) 176
BclI TGATCA 2 cut(s) 51, 462
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 4 cut(s) 121, 238, 324, 415
BfaI CTAG 1 cut(s) 610
BisI GCNGC 2 cut(s) 265, 433
BlsI GCNGC 2 cut(s) 266, 434
Bme1390I CCNGG 2 cut(s) 176, 201
Bme18I GGWCC 1 cut(s) 390
BmgT120I GGNCC 1 cut(s) 390
BmiI GGNNCC 1 cut(s) 392
BmrFI CCNGG 2 cut(s) 176, 201
BmsI GCATC 1 cut(s) 573
BpiI GAAGAC 2 cut(s) 48, 254
BplI GAGNNNNNCTC 2 cut(s) 77, 109
BpuEI CTTGAG 1 cut(s) 104
BpuMI CCSGG 1 cut(s) 201
BsaAI YACGTR 1 cut(s) 271
BsaJI CCNNGG 4 cut(s) 105, 121, 174, 546
BsaXI ACNNNNNCTCC 2 cut(s) 409, 439
Bsc4I CCNNNNNNNGG 1 cut(s) 400
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 4 cut(s) 105, 121, 174, 546
BseLI CCNNNNNNNGG 1 cut(s) 400
BseMII CTCAG 1 cut(s) 144
BseRI GAGGAG 4 cut(s) 152, 182, 430, 433
BseSI GKGCMC 1 cut(s) 201
BseXI GCAGC 2 cut(s) 251, 444
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 1 cut(s) 135
BsiSI CCGG 1 cut(s) 201
BsiWI CGTACG 1 cut(s) 425
BslI CCNNNNNNNGG 1 cut(s) 400
BsmAI GTCTC 4 cut(s) 121, 238, 324, 415
BsmBI CGTCTC 1 cut(s) 415
BsnI GGCC 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 5 cut(s) 51, 157, 275, 350, 462
BspACI CCGC 2 cut(s) 105, 107
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 1 cut(s) 143
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 1 cut(s) 392
BspPI GGATC 1 cut(s) 270
BspQI GCTCTTC 1 cut(s) 471
BssECI CCNNGG 4 cut(s) 105, 121, 174, 546
BssMI GATC 5 cut(s) 51, 157, 275, 350, 462
BssSI CACGAG 1 cut(s) 194
BssT1I CCWWGG 1 cut(s) 546
Bst2BI CACGAG 1 cut(s) 194
Bst2UI CCWGG 1 cut(s) 176
Bst4CI ACNGT 2 cut(s) 11, 74
Bst6I CTCTTC 2 cut(s) 30, 471
BstBAI YACGTR 1 cut(s) 271
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 1 cut(s) 130
BstDSI CCRYGG 2 cut(s) 105, 121
BstFNI CGCG 1 cut(s) 107
BstKTI GATC 5 cut(s) 54, 160, 278, 353, 465
BstMAI GTCTC 4 cut(s) 121, 238, 324, 415
BstMBI GATC 5 cut(s) 51, 157, 275, 350, 462
BstNI CCWGG 1 cut(s) 176
BstSCI CCNGG 2 cut(s) 174, 199
BstSLI GKGCMC 1 cut(s) 201
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 2 cut(s) 251, 444
BstV2I GAAGAC 2 cut(s) 48, 254
BstX2I RGATCY 1 cut(s) 275
BstYI RGATCY 1 cut(s) 275
BsuRI GGCC 1 cut(s) 135
BtgI CCRYGG 2 cut(s) 105, 121
BtsIMutI CAGTG 1 cut(s) 471
Cac8I GCNNGC 1 cut(s) 109
CaiI CAGNNNCTG 1 cut(s) 493
Cfr13I GGNCC 1 cut(s) 390
Cfr42I CCGCGG 1 cut(s) 108
Csp6I GTAC 3 cut(s) 310, 426, 602
CviAII CATG 1 cut(s) 137
CviQI GTAC 3 cut(s) 310, 426, 602
DdeI CTNAG 1 cut(s) 130
DpnI GATC 5 cut(s) 53, 159, 277, 352, 464
DpnII GATC 5 cut(s) 51, 157, 275, 350, 462
DraIII CACNNNGTG 1 cut(s) 340
Eam1104I CTCTTC 2 cut(s) 30, 471
EarI CTCTTC 2 cut(s) 30, 471
Eco130I CCWWGG 1 cut(s) 546
Eco47I GGWCC 1 cut(s) 390
Eco57I CTGAAG 2 cut(s) 495, 519
Eco72I CACGTG 1 cut(s) 271
EcoO109I RGGNCCY 1 cut(s) 390
EcoRII CCWGG 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 546
ErhI CCWWGG 1 cut(s) 546
Esp3I CGTCTC 1 cut(s) 415
FaeI CATG 1 cut(s) 140
FaiI YATR 6 cut(s) 62, 138, 355, 386, 448, 467
FatI CATG 1 cut(s) 136
FauI CCCGC 1 cut(s) 100
FbaI TGATCA 2 cut(s) 51, 462
Fnu4HI GCNGC 2 cut(s) 265, 433
Fsp4HI GCNGC 2 cut(s) 265, 433
FspBI CTAG 1 cut(s) 610
GluI GCNGC 2 cut(s) 265, 433
HaeIII GGCC 1 cut(s) 135
HapII CCGG 1 cut(s) 201
Hin1II CATG 1 cut(s) 140
HinfI GANTC 4 cut(s) 126, 287, 457, 490
HpaII CCGG 1 cut(s) 201
HphI GGTGA 1 cut(s) 563
Hpy188I TCNGA 6 cut(s) 20, 70, 238, 462, 499, 583
Hpy188III TCNNGA 5 cut(s) 83, 166, 182, 323, 372
Hpy99I CGWCG 3 cut(s) 35, 74, 242
HpyCH4III ACNGT 2 cut(s) 11, 74
HpyCH4IV ACGT 3 cut(s) 270, 363, 424
HpyCH4V TGCA 1 cut(s) 443
HpyF3I CTNAG 1 cut(s) 130
HpySE526I ACGT 3 cut(s) 270, 363, 424
Hsp92II CATG 1 cut(s) 140
Ksp22I TGATCA 2 cut(s) 51, 462
KspI CCGCGG 1 cut(s) 108
Kzo9I GATC 5 cut(s) 51, 157, 275, 350, 462
LguI GCTCTTC 1 cut(s) 471
LpnPI CCDG 7 cut(s) 117, 151, 161, 188, 214, 308, 322
Lsp1109I GCAGC 2 cut(s) 251, 444
LweI GCATC 1 cut(s) 573
MaeI CTAG 1 cut(s) 610
MaeII ACGT 3 cut(s) 270, 363, 424
MaeIII GTNAC 2 cut(s) 100, 483
MalI GATC 5 cut(s) 53, 159, 277, 352, 464
MboI GATC 5 cut(s) 51, 157, 275, 350, 462
MboII GAAGA 7 cut(s) 47, 53, 259, 418, 488, 512, 519
MflI RGATCY 1 cut(s) 275
MhlI GDGCHC 1 cut(s) 201
MluCI AATT 1 cut(s) 586
MlyI GAGTC 1 cut(s) 296
MmeI TCCRAC 3 cut(s) 93, 261, 303
MnlI CCTC 7 cut(s) 14, 31, 173, 200, 203, 408, 411
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 2 cut(s) 176, 201
MvaI CCWGG 1 cut(s) 176
MvnI CGCG 1 cut(s) 107
NciI CCSGG 1 cut(s) 201
NdeII GATC 5 cut(s) 51, 157, 275, 350, 462
NlaIII CATG 1 cut(s) 140
NlaIV GGNNCC 1 cut(s) 392
NmeAIII GCCGAG 1 cut(s) 128
PciSI GCTCTTC 1 cut(s) 471
PfeI GAWTC 3 cut(s) 126, 457, 490
Pfl23II CGTACG 1 cut(s) 425
PkrI GCNGC 2 cut(s) 266, 434
PleI GAGTC 1 cut(s) 295
PmaCI CACGTG 1 cut(s) 271
PmlI CACGTG 1 cut(s) 271
PpsI GAGTC 1 cut(s) 295
Ppu21I YACGTR 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 390
Psp5II RGGWCCY 1 cut(s) 390
Psp6I CCWGG 1 cut(s) 174
PspCI CACGTG 1 cut(s) 271
PspGI CCWGG 1 cut(s) 174
PspLI CGTACG 1 cut(s) 425
PspN4I GGNNCC 1 cut(s) 392
PspPI GGNCC 1 cut(s) 390
PspPPI RGGWCCY 1 cut(s) 390
PstNI CAGNNNCTG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 275
RsaI GTAC 3 cut(s) 311, 427, 603
RsaNI GTAC 3 cut(s) 310, 426, 602
SacII CCGCGG 1 cut(s) 108
SapI GCTCTTC 1 cut(s) 471
SatI GCNGC 2 cut(s) 265, 433
Sau3AI GATC 5 cut(s) 51, 157, 275, 350, 462
Sau96I GGNCC 1 cut(s) 390
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 2 cut(s) 176, 201
SduI GDGCHC 1 cut(s) 201
SetI ASST 9 cut(s) 192, 266, 273, 366, 427, 442, 483, 522, 611
SfaNI GCATC 1 cut(s) 573
Sfr303I CCGCGG 1 cut(s) 108
SgrBI CCGCGG 1 cut(s) 108
SinI GGWCC 1 cut(s) 390
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
Sse9I AATT 1 cut(s) 586
SsiI CCGC 2 cut(s) 105, 107
SspI AATATT 1 cut(s) 229
SspMI CTAG 1 cut(s) 610
StyD4I CCNGG 2 cut(s) 174, 199
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 2 cut(s) 11, 74
TaiI ACGT 3 cut(s) 273, 366, 427
TaqI TCGA 1 cut(s) 560
TasI AATT 1 cut(s) 586
TfiI GAWTC 3 cut(s) 126, 457, 490
TscAI CASTG 1 cut(s) 478
TseI GCWGC 2 cut(s) 264, 432
TspGWI ACGGA 3 cut(s) 138, 297, 374
TspRI CASTG 1 cut(s) 478
VpaK11BI GGWCC 1 cut(s) 390
XapI RAATTY 1 cut(s) 586
XspI CTAG 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.