MD14G1138100.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015004
Physical Location & Seq
Reverse (-)
22579338 .. 22580052
715 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1138100.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGGCAATTGCTTGTTTGGAGGTTTGGGAGATGCAGATGGGGTAGTTCAAGTGACAACATCAAATGGTGGGATAATGGAATTTTATGTGCCAATCACTGTAGGCTTGATCACGAAGGAATTTCCAGGCCATGGAATTTTCAGAAGCCATGATCTTTTCTGGAAACCACTCTCTCACCATGATGATCTTCTGGCAGGACAAAATTACTATCTTCTCCCTCTCAACAGCCAAAGTGGTCGTCACTTGGGCAGCGCCCAGATTGTGAGGGAAGGCCATGTTCGGTCAAACAGCATACCAACTTGTTCACTTGCTGCGCCCTACAGAATGTCGCTTGATTACCATGGAATGATGAAGAGGTCTTACACCGAGGCATTTTCGAAGTACAATAACGTCAGTTTTTGGAAGGTGAAGCTGGTGATCAGTCCAGAGCACCTGTTGGAGATTTTGGCTCAAGAGGGTCGGACTCAGGAATTGATTGAGAGTGTAAGGACTGTGGCTAAATGTGGGAGTGGTGGTTCGGTTTCAGGTTTGGGATTTTCCGATCAGTGGAGCCTGTCGAGTAGTAGTAGAAATGCTTCATCTAAGAAAGATGGAATATTACTAGATATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

204

Amino Acids

22.2

Weight (kDa)

7.09

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 163 3.8e-18 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015004)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64700
fragaria_vesca FvH4_5g10680
malus_domestica MD06G1121500.v1.1 MD14G1138100.v1.1
prunus_persica Prupe.5G132000_v2.0.a1
pyrus_communis pycom14g11320
rosa_chinensis RchiOBHm_Chr7g0187471
rosa_laevigata RLG00000004778
rosa_multiflora Rmu_sc0001230.1_g000008
rosa_roxburghii Rroxscaffold_3G00267400
rosa_rugosa Rorug06G0481800
rosa_samantha Rh7AG086300 Rh7BG087900 Rh7CG087300 Rh7DG088900
rosa_wichuraiana Rw7G007620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 131
AcsI RAATTY 3 cut(s) 80, 119, 135
AfaI GTAC 1 cut(s) 383
AfiI CCNNNNNNNGG 2 cut(s) 131, 546
AgsI TTSAA 1 cut(s) 50
AjnI CCWGG 1 cut(s) 124
AloI GAACNNNNNNTCC 2 cut(s) 499, 531
AluBI AGCT 1 cut(s) 412
AluI AGCT 1 cut(s) 412
Alw21I GWGCWC 1 cut(s) 432
AoxI GGCC 2 cut(s) 127, 271
ApeKI GCWGC 2 cut(s) 249, 311
ApoI RAATTY 3 cut(s) 80, 119, 135
ArsI GACNNNNNNTTYG 2 cut(s) 223, 255
Asp700I GAANNNNTTC 1 cut(s) 574
AspLEI GCGC 2 cut(s) 254, 316
AsuHPI GGTGA 3 cut(s) 167, 418, 427
AsuII TTCGAA 1 cut(s) 377
BarI GAAGNNNNNNTAC 4 cut(s) 344, 376, 559, 591
Bbv12I GWGCWC 1 cut(s) 432
BbvI GCAGC 2 cut(s) 261, 298
BccI CCATC 2 cut(s) 32, 584
BciT130I CCWGG 1 cut(s) 126
BclI TGATCA 2 cut(s) 108, 417
BfaI CTAG 1 cut(s) 602
BfmI CTRYAG 2 cut(s) 99, 319
BfoI RGCGCY 1 cut(s) 255
BisI GCNGC 2 cut(s) 250, 312
BlsI GCNGC 2 cut(s) 251, 313
Bme1390I CCNGG 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 551
BmrFI CCNGG 1 cut(s) 126
BmsI GCATC 1 cut(s) 22
Bpu14I TTCGAA 1 cut(s) 377
BpuEI CTTGAG 1 cut(s) 435
BsaJI CCNNGG 3 cut(s) 130, 340, 366
BsaXI ACNNNNNCTCC 2 cut(s) 499, 529
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 546
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 3 cut(s) 130, 340, 366
BseLI CCNNNNNNNGG 2 cut(s) 131, 546
BseMII CTCAG 1 cut(s) 479
BseXI GCAGC 2 cut(s) 261, 298
BshFI GGCC 2 cut(s) 129, 273
BsiHKAI GWGCWC 1 cut(s) 432
BslI CCNNNNNNNGG 2 cut(s) 131, 546
BsnI GGCC 2 cut(s) 129, 273
Bsp119I TTCGAA 1 cut(s) 377
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 5 cut(s) 108, 151, 184, 417, 541
Bsp19I CCATGG 2 cut(s) 130, 340
BspANI GGCC 2 cut(s) 129, 273
BspCNI CTCAG 1 cut(s) 478
BspLI GGNNCC 1 cut(s) 551
BspT104I TTCGAA 1 cut(s) 377
BssECI CCNNGG 3 cut(s) 130, 340, 366
BssMI GATC 5 cut(s) 108, 151, 184, 417, 541
BssT1I CCWWGG 2 cut(s) 130, 340
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 2 cut(s) 100, 493
Bst6I CTCTTC 1 cut(s) 347
BstBI TTCGAA 1 cut(s) 377
BstDEI CTNAG 2 cut(s) 465, 582
BstDSI CCRYGG 2 cut(s) 130, 340
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 2 cut(s) 254, 316
BstKTI GATC 5 cut(s) 111, 154, 187, 420, 544
BstMBI GATC 5 cut(s) 108, 151, 184, 417, 541
BstNI CCWGG 1 cut(s) 126
BstSCI CCNGG 1 cut(s) 124
BstSFI CTRYAG 2 cut(s) 99, 319
BstV1I GCAGC 2 cut(s) 261, 298
BsuRI GGCC 2 cut(s) 129, 273
BtgI CCRYGG 2 cut(s) 130, 340
BtsIMutI CAGTG 2 cut(s) 96, 551
CfoI GCGC 2 cut(s) 254, 316
Csp6I GTAC 1 cut(s) 382
CviAII CATG 5 cut(s) 131, 149, 179, 275, 341
CviJI RGCY 9 cut(s) 105, 129, 147, 228, 273, 412, 449, 497, 552
CviKI_1 RGCY 9 cut(s) 105, 129, 147, 228, 273, 412, 449, 497, 552
CviQI GTAC 1 cut(s) 382
DdeI CTNAG 2 cut(s) 465, 582
DpnI GATC 5 cut(s) 110, 153, 186, 419, 543
DpnII GATC 5 cut(s) 108, 151, 184, 417, 541
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
Eco130I CCWWGG 2 cut(s) 130, 340
EcoRII CCWGG 1 cut(s) 124
EcoT14I CCWWGG 2 cut(s) 130, 340
ErhI CCWWGG 2 cut(s) 130, 340
FaeI CATG 5 cut(s) 134, 152, 182, 278, 344
FaiI YATR 7 cut(s) 87, 132, 150, 180, 276, 293, 342
FatI CATG 5 cut(s) 130, 148, 178, 274, 340
FbaI TGATCA 2 cut(s) 108, 417
Fnu4HI GCNGC 2 cut(s) 250, 312
Fsp4HI GCNGC 2 cut(s) 250, 312
FspBI CTAG 1 cut(s) 602
GlaI GCGC 2 cut(s) 253, 315
GluI GCNGC 2 cut(s) 250, 312
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 2 cut(s) 129, 273
HhaI GCGC 2 cut(s) 254, 316
Hin1II CATG 5 cut(s) 134, 152, 182, 278, 344
Hin6I GCGC 2 cut(s) 252, 314
HinP1I GCGC 2 cut(s) 252, 314
HinfI GANTC 1 cut(s) 463
HphI GGTGA 3 cut(s) 167, 418, 427
Hpy166II GTNNAC 1 cut(s) 305
Hpy188I TCNGA 3 cut(s) 143, 462, 541
Hpy188III TCNNGA 5 cut(s) 112, 160, 425, 452, 467
Hpy8I GTNNAC 1 cut(s) 305
HpyAV CCTTC 3 cut(s) 109, 263, 397
HpyCH4III ACNGT 2 cut(s) 100, 493
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 1 cut(s) 35
HpyF3I CTNAG 2 cut(s) 465, 582
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 5 cut(s) 134, 152, 182, 278, 344
HspAI GCGC 2 cut(s) 252, 314
Ksp22I TGATCA 2 cut(s) 108, 417
Kzo9I GATC 5 cut(s) 108, 151, 184, 417, 541
LmnI GCTCC 1 cut(s) 549
Lsp1109I GCAGC 2 cut(s) 261, 298
LweI GCATC 1 cut(s) 22
MaeI CTAG 1 cut(s) 602
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 2 cut(s) 52, 239
MalI GATC 5 cut(s) 110, 153, 186, 419, 543
MboI GATC 5 cut(s) 108, 151, 184, 417, 541
MboII GAAGA 3 cut(s) 179, 203, 364
MfeI CAATTG 1 cut(s) 7
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 6 cut(s) 7, 80, 119, 135, 202, 470
MlyI GAGTC 1 cut(s) 457
MmeI TCCRAC 2 cut(s) 417, 440
MnlI CCTC 6 cut(s) 14, 228, 258, 348, 361, 448
MroXI GAANNNNTTC 1 cut(s) 574
MslI CAYNNNNRTG 1 cut(s) 180
MspR9I CCNGG 1 cut(s) 126
MunI CAATTG 1 cut(s) 7
MvaI CCWGG 1 cut(s) 126
NcoI CCATGG 2 cut(s) 130, 340
NdeII GATC 5 cut(s) 108, 151, 184, 417, 541
NlaIII CATG 5 cut(s) 134, 152, 182, 278, 344
NlaIV GGNNCC 1 cut(s) 551
NmuCI GTSAC 2 cut(s) 52, 239
NspV TTCGAA 1 cut(s) 377
PdmI GAANNNNTTC 1 cut(s) 574
PflMI CCANNNNNTGG 1 cut(s) 131
PkrI GCNGC 2 cut(s) 251, 313
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 551
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
RseI CAYNNNNRTG 1 cut(s) 180
SatI GCNGC 2 cut(s) 250, 312
Sau3AI GATC 5 cut(s) 108, 151, 184, 417, 541
SchI GAGTC 1 cut(s) 457
ScrFI CCNGG 1 cut(s) 126
SduI GDGCHC 1 cut(s) 432
SetI ASST 7 cut(s) 25, 359, 393, 408, 414, 435, 529
SfaNI GCATC 1 cut(s) 22
SfcI CTRYAG 2 cut(s) 99, 319
SfuI TTCGAA 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 180
SmlI CTYRAG 1 cut(s) 450
SmoI CTYRAG 1 cut(s) 450
Sse9I AATT 6 cut(s) 7, 80, 119, 135, 202, 470
SspI AATATT 1 cut(s) 597
SspMI CTAG 1 cut(s) 602
StyD4I CCNGG 1 cut(s) 124
StyI CCWWGG 2 cut(s) 130, 340
TaaI ACNGT 2 cut(s) 100, 493
TaiI ACGT 1 cut(s) 393
TaqI TCGA 2 cut(s) 377, 557
TaqII GACCGA 1 cut(s) 270
TasI AATT 6 cut(s) 7, 80, 119, 135, 202, 470
TatI WGTACW 1 cut(s) 381
TscAI CASTG 2 cut(s) 103, 551
TseFI GTSAC 2 cut(s) 52, 239
TseI GCWGC 2 cut(s) 249, 311
Tsp45I GTSAC 2 cut(s) 52, 239
TspDTI ATGAA 2 cut(s) 365, 567
TspRI CASTG 2 cut(s) 103, 551
Van91I CCANNNNNTGG 1 cut(s) 131
XapI RAATTY 3 cut(s) 80, 119, 135
XmnI GAANNNNTTC 1 cut(s) 574
XspI CTAG 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.