FvH4_5g10680

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015004
Physical Location & Seq
Forward (+)
6065879 .. 6067093
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10680.t1

Sequence Viewer

Length: 633 bp
ATGGGAAACTGCTTGTTTGGAGGTATGGGAGATGCAGCTGGTGGAGTAGTCCAAGTGACGACGGCCAATGGCGGCATCATGGAGTTTTACACGCCTATTACAGTAAGCTCCATCACCGACGAGTTTCCCAGCCATGGAATTTTCCGAAGCCATGATCTCTTCTGGAAGCCGCTCGCTAACAGCGAAGAACTTGTGGCAGGCCAGTCATATTATTTGCTCCCACTCAACAGCCAAAGCAATCACTTGGGAAGTAGTAGTACTAATCAGCTTGTGCGTCAAGGCCATGTTAGATCCAACAGCATACCTTTACCTACTTTGCTTGCCGGAGCGCCGTACCGGATGTCGCTTGATTACCAGGGAATGGTGAGGAAATCTTACACTGAAGCGTTTTCGAGGTATAATAATGTGAGCTTTTGGAAGGTGAAGCTGGTGATTAGCCCTGAACAGCTTTTGGAGATACTATCGCAGGAGGGTCGGACGCAGGAGTTGATTGAGAGCGTGAGGACGGTGGCAAAATGTGGGAGTGGTTCGGTATCGGCTGCCGGGGGATTTTCCGATCAGTGGAGTCTTTCCAGTAGTAGCAGGAATGCTTCTTCTACTTCCAAGAAAGATTTTGGATTGGTAGACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

211

Amino Acids

22.71

Weight (kDa)

6.41

Isoelectric Point (pI)

46.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 168 1.6e-18 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015004)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64700
fragaria_vesca FvH4_5g10680
malus_domestica MD06G1121500.v1.1 MD14G1138100.v1.1
prunus_persica Prupe.5G132000_v2.0.a1
pyrus_communis pycom14g11320
rosa_chinensis RchiOBHm_Chr7g0187471
rosa_laevigata RLG00000004778
rosa_multiflora Rmu_sc0001230.1_g000008
rosa_roxburghii Rroxscaffold_3G00267400
rosa_rugosa Rorug06G0481800
rosa_samantha Rh7AG086300 Rh7BG087900 Rh7CG087300 Rh7DG088900
rosa_wichuraiana Rw7G007620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 361
AccBSI CCGCTC 1 cut(s) 172
AccI GTMKAC 1 cut(s) 624
AciI CCGC 2 cut(s) 72, 170
AclWI GGATC 1 cut(s) 285
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 138
AcuI CTGAAG 1 cut(s) 402
AfaI GTAC 2 cut(s) 259, 335
AfiI CCNNNNNNNGG 3 cut(s) 134, 361, 561
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 6 cut(s) 38, 108, 268, 411, 427, 448
AluI AGCT 6 cut(s) 38, 108, 268, 411, 427, 448
AlwI GGATC 1 cut(s) 285
AoxI GGCC 3 cut(s) 63, 199, 280
ApeKI GCWGC 2 cut(s) 35, 539
ApoI RAATTY 1 cut(s) 138
AspLEI GCGC 1 cut(s) 331
AsuC2I CCSGG 1 cut(s) 544
AsuHPI GGTGA 4 cut(s) 106, 376, 433, 442
BarI GAAGNNNNNNTAC 2 cut(s) 241, 273
BbvI GCAGC 2 cut(s) 47, 526
BccI CCATC 1 cut(s) 119
BceAI ACGGC 2 cut(s) 78, 316
BcgI CGANNNNNNTGC 2 cut(s) 455, 489
BciT130I CCWGG 1 cut(s) 356
BcnI CCSGG 1 cut(s) 544
BfoI RGCGCY 1 cut(s) 332
BisI GCNGC 4 cut(s) 36, 73, 170, 540
BlsI GCNGC 4 cut(s) 37, 74, 171, 541
BmcAI AGTACT 1 cut(s) 259
Bme1390I CCNGG 2 cut(s) 356, 544
BmrFI CCNGG 2 cut(s) 356, 544
BmsI GCATC 2 cut(s) 22, 84
BpuMI CCSGG 1 cut(s) 544
BsaJI CCNNGG 3 cut(s) 133, 355, 543
BsaWI WCCGGW 1 cut(s) 336
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 361, 561
Bse1I ACTGG 2 cut(s) 202, 573
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 3 cut(s) 133, 355, 543
BseGI GGATG 1 cut(s) 345
BseLI CCNNNNNNNGG 3 cut(s) 134, 361, 561
BseNI ACTGG 2 cut(s) 202, 573
BseXI GCAGC 2 cut(s) 47, 526
BseYI CCCAGC 1 cut(s) 128
BshFI GGCC 3 cut(s) 65, 201, 282
BsiSI CCGG 3 cut(s) 324, 337, 543
BslI CCNNNNNNNGG 3 cut(s) 134, 361, 561
BsmI GAATGC 1 cut(s) 592
BsnI GGCC 3 cut(s) 65, 201, 282
Bsp143I GATC 3 cut(s) 154, 290, 556
Bsp19I CCATGG 1 cut(s) 133
BspACI CCGC 2 cut(s) 72, 170
BspANI GGCC 3 cut(s) 65, 201, 282
BspPI GGATC 1 cut(s) 285
BsrBI CCGCTC 1 cut(s) 172
BsrI ACTGG 2 cut(s) 202, 573
BssECI CCNNGG 3 cut(s) 133, 355, 543
BssMI GATC 3 cut(s) 154, 290, 556
BssT1I CCWWGG 1 cut(s) 133
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 2 cut(s) 103, 508
Bst6I CTCTTC 1 cut(s) 164
BstC8I GCNNGC 3 cut(s) 174, 199, 321
BstDSI CCRYGG 1 cut(s) 133
BstF5I GGATG 1 cut(s) 345
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 3 cut(s) 157, 293, 559
BstMBI GATC 3 cut(s) 154, 290, 556
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 2 cut(s) 354, 542
BstV1I GCAGC 2 cut(s) 47, 526
BstX2I RGATCY 1 cut(s) 290
BstYI RGATCY 1 cut(s) 290
BsuRI GGCC 3 cut(s) 65, 201, 282
BtgI CCRYGG 1 cut(s) 133
BtsCI GGATG 1 cut(s) 345
BtsIMutI CAGTG 2 cut(s) 378, 566
Cac8I GCNNGC 3 cut(s) 174, 199, 321
CfoI GCGC 1 cut(s) 331
CseI GACGC 2 cut(s) 263, 487
Csp6I GTAC 2 cut(s) 258, 334
CviAII CATG 4 cut(s) 79, 134, 152, 284
CviQI GTAC 2 cut(s) 258, 334
DpnI GATC 3 cut(s) 156, 292, 558
DpnII GATC 3 cut(s) 154, 290, 556
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 402
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 133
FaeI CATG 4 cut(s) 82, 137, 155, 287
FaiI YATR 8 cut(s) 26, 80, 135, 153, 208, 285, 302, 399
FatI CATG 4 cut(s) 78, 133, 151, 283
FblI GTMKAC 1 cut(s) 624
Fnu4HI GCNGC 4 cut(s) 36, 73, 170, 540
FokI GGATG 1 cut(s) 352
Fsp4HI GCNGC 4 cut(s) 36, 73, 170, 540
GlaI GCGC 1 cut(s) 330
GluI GCNGC 4 cut(s) 36, 73, 170, 540
GsaI CCCAGC 1 cut(s) 132
HaeII RGCGCY 1 cut(s) 332
HaeIII GGCC 3 cut(s) 65, 201, 282
HapII CCGG 3 cut(s) 324, 337, 543
HgaI GACGC 2 cut(s) 263, 487
HhaI GCGC 1 cut(s) 331
Hin1II CATG 4 cut(s) 82, 137, 155, 287
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HinfI GANTC 1 cut(s) 565
HpaII CCGG 3 cut(s) 324, 337, 543
HphI GGTGA 4 cut(s) 106, 376, 433, 442
Hpy166II GTNNAC 1 cut(s) 625
Hpy188I TCNGA 3 cut(s) 146, 477, 556
Hpy188III TCNNGA 1 cut(s) 163
Hpy8I GTNNAC 1 cut(s) 625
Hpy99I CGWCG 2 cut(s) 64, 122
HpyAV CCTTC 1 cut(s) 412
HpyCH4III ACNGT 2 cut(s) 103, 508
HpyCH4V TGCA 1 cut(s) 35
Hsp92II CATG 4 cut(s) 82, 137, 155, 287
HspAI GCGC 1 cut(s) 329
Kzo9I GATC 3 cut(s) 154, 290, 556
LmnI GCTCC 3 cut(s) 113, 222, 326
Lsp1109I GCAGC 2 cut(s) 47, 526
LweI GCATC 2 cut(s) 22, 84
MaeIII GTNAC 1 cut(s) 55
MalI GATC 3 cut(s) 156, 292, 558
MbiI CCGCTC 1 cut(s) 172
MboI GATC 3 cut(s) 154, 290, 556
MboII GAAGA 3 cut(s) 151, 197, 585
MflI RGATCY 1 cut(s) 290
MluCI AATT 1 cut(s) 138
MlyI GAGTC 1 cut(s) 574
MmeI TCCRAC 2 cut(s) 318, 455
MnlI CCTC 5 cut(s) 14, 360, 387, 463, 495
MspA1I CMGCKG 1 cut(s) 38
MspI CCGG 3 cut(s) 324, 337, 543
MspR9I CCNGG 2 cut(s) 356, 544
Mva1269I GAATGC 1 cut(s) 592
MvaI CCWGG 1 cut(s) 356
NciI CCSGG 1 cut(s) 544
NcoI CCATGG 1 cut(s) 133
NdeII GATC 3 cut(s) 154, 290, 556
NlaIII CATG 4 cut(s) 82, 137, 155, 287
NmuCI GTSAC 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PctI GAATGC 1 cut(s) 592
PflMI CCANNNNNTGG 1 cut(s) 361
PkrI GCNGC 4 cut(s) 37, 74, 171, 541
PleI GAGTC 1 cut(s) 573
PpsI GAGTC 1 cut(s) 573
Psp6I CCWGG 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 128
PspGI CCWGG 1 cut(s) 354
PsuI RGATCY 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 38
RsaI GTAC 2 cut(s) 259, 335
RsaNI GTAC 2 cut(s) 258, 334
SatI GCNGC 4 cut(s) 36, 73, 170, 540
Sau3AI GATC 3 cut(s) 154, 290, 556
ScaI AGTACT 1 cut(s) 259
SchI GAGTC 1 cut(s) 574
ScrFI CCNGG 2 cut(s) 356, 544
SfaNI GCATC 2 cut(s) 22, 84
Sse9I AATT 1 cut(s) 138
SsiI CCGC 2 cut(s) 72, 170
StyD4I CCNGG 2 cut(s) 354, 542
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 2 cut(s) 103, 508
TaqI TCGA 1 cut(s) 392
TasI AATT 1 cut(s) 138
TatI WGTACW 1 cut(s) 257
TauI GCSGC 2 cut(s) 75, 172
TscAI CASTG 2 cut(s) 385, 566
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 2 cut(s) 35, 539
Tsp45I GTSAC 1 cut(s) 55
TspRI CASTG 2 cut(s) 385, 566
Van91I CCANNNNNTGG 1 cut(s) 361
XapI RAATTY 1 cut(s) 138
XmiI GTMKAC 1 cut(s) 624
ZrmI AGTACT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.