Prupe.5G132000_v2.0.a1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0015004
Physical Location & Seq
Reverse (-)
12701224 .. 12702250
1027 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G132000.1

Sequence Viewer

Length: 609 bp
ATGGGAAATTGCTTGTTTGGGGGTGTGGGGGAGGCAGAAGGGTTGGTCCAAGTGACAACTTCCAATGGTGGCATAATGGAATTCTATGCGCCAATCACAGTAGGCTCAATCACCAAAGAATTTCCAAACCATGGAATTTTCAGAAGCCATGACCTCTTCTGGAAACCACTCTCTCCCCATGAAGATCTTGTGGCAAGACAAAACTACTATCTCCTCCCACTCACCAGCCAAAGTGGTCACTTGGGCAGTGCCCAAATTGTGAGGGAAGGCCATGTGAGATCAAACAGCATACCTACTTGCTCCCTTTCTGCACCATACAGAATGTCGCTTGATTATCAAGGCATGATGAAGAGGTCTTACACCGAAGCTTTTTCAAGGTACAACAATGTCAGCTTTTGGAAGGTGAAGCTTGTGATCAGCCCAGAGCAGCTGCTGGAGATTTTGGCTCAGGAGGGAAGAACCCAGGAGTTGATTGAGAGCGTGAGGACTGTGGCTAAGTGTGGGAATGGTGGCTCTGCCTCTGGTTTGGGATTTTCTGACCAGTGGAGTCTCTCAAGTAGTAGTAGAAATGCTTCGTCTAAGAAAGATGGAATATTGCTAGACATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

203

Amino Acids

22.14

Weight (kDa)

6.96

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015004)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64700
fragaria_vesca FvH4_5g10680
malus_domestica MD06G1121500.v1.1 MD14G1138100.v1.1
prunus_persica Prupe.5G132000_v2.0.a1
pyrus_communis pycom14g11320
rosa_chinensis RchiOBHm_Chr7g0187471
rosa_laevigata RLG00000004778
rosa_multiflora Rmu_sc0001230.1_g000008
rosa_roxburghii Rroxscaffold_3G00267400
rosa_rugosa Rorug06G0481800
rosa_samantha Rh7AG086300 Rh7BG087900 Rh7CG087300 Rh7DG088900
rosa_wichuraiana Rw7G007620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 131
AcsI RAATTY 3 cut(s) 80, 119, 135
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 1 cut(s) 131
AgsI TTSAA 1 cut(s) 375
AjnI CCWGG 1 cut(s) 462
AluBI AGCT 4 cut(s) 368, 393, 409, 430
AluI AGCT 4 cut(s) 368, 393, 409, 430
Alw26I GTCTC 1 cut(s) 554
AlwNI CAGNNNCTG 1 cut(s) 433
AoxI GGCC 1 cut(s) 268
ApeKI GCWGC 2 cut(s) 427, 430
ApoI RAATTY 3 cut(s) 80, 119, 135
Asp700I GAANNNNTTC 1 cut(s) 571
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 1 cut(s) 46
AsuHPI GGTGA 3 cut(s) 103, 214, 415
AvaII GGWCC 1 cut(s) 46
BaeGI GKGCMC 1 cut(s) 253
BarI GAAGNNNNNNTAC 4 cut(s) 341, 373, 556, 588
BbvI GCAGC 2 cut(s) 417, 439
BccI CCATC 1 cut(s) 581
BciT130I CCWGG 1 cut(s) 464
BclI TGATCA 1 cut(s) 414
BcoDI GTCTC 1 cut(s) 554
BfaI CTAG 1 cut(s) 599
BglII AGATCT 1 cut(s) 184
BisI GCNGC 2 cut(s) 428, 431
BlsI GCNGC 2 cut(s) 429, 432
Bme1390I CCNGG 1 cut(s) 464
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 46
BmrFI CCNGG 1 cut(s) 464
BpmI CTGGAG 1 cut(s) 455
Bpu10I CCTNAGC 1 cut(s) 447
BpuEI CTTGAG 1 cut(s) 538
BsaJI CCNNGG 2 cut(s) 130, 462
Bsc4I CCNNNNNNNGG 1 cut(s) 131
Bse1I ACTGG 1 cut(s) 541
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 2 cut(s) 130, 462
BseLI CCNNNNNNNGG 1 cut(s) 131
BseMII CTCAG 1 cut(s) 461
BseNI ACTGG 1 cut(s) 541
BseRI GAGGAG 1 cut(s) 203
BseSI GKGCMC 1 cut(s) 253
BseXI GCAGC 2 cut(s) 417, 439
BsgI GTGCAG 1 cut(s) 294
BshFI GGCC 1 cut(s) 270
BslI CCNNNNNNNGG 1 cut(s) 131
BsmAI GTCTC 1 cut(s) 554
BsnI GGCC 1 cut(s) 270
Bsp1286I GDGCHC 1 cut(s) 253
Bsp143I GATC 3 cut(s) 184, 278, 414
Bsp19I CCATGG 1 cut(s) 130
BspANI GGCC 1 cut(s) 270
BspCNI CTCAG 1 cut(s) 460
BsrI ACTGG 1 cut(s) 541
BssECI CCNNGG 2 cut(s) 130, 462
BssMI GATC 3 cut(s) 184, 278, 414
BssT1I CCWWGG 1 cut(s) 130
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 2 cut(s) 100, 490
Bst6I CTCTTC 2 cut(s) 161, 344
BstDEI CTNAG 3 cut(s) 447, 495, 579
BstDSI CCRYGG 1 cut(s) 130
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 3 cut(s) 187, 281, 417
BstMAI GTCTC 1 cut(s) 554
BstMBI GATC 3 cut(s) 184, 278, 414
BstNI CCWGG 1 cut(s) 464
BstSCI CCNGG 1 cut(s) 462
BstSLI GKGCMC 1 cut(s) 253
BstV1I GCAGC 2 cut(s) 417, 439
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BsuRI GGCC 1 cut(s) 270
BtgI CCRYGG 1 cut(s) 130
BtsI GCAGTG 1 cut(s) 253
BtsIMutI CAGTG 2 cut(s) 253, 548
CaiI CAGNNNCTG 1 cut(s) 433
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 1 cut(s) 46
Csp6I GTAC 1 cut(s) 379
CviAII CATG 5 cut(s) 131, 149, 179, 272, 343
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 3 cut(s) 447, 495, 579
DpnI GATC 3 cut(s) 186, 280, 416
DpnII GATC 3 cut(s) 184, 278, 414
Eam1104I CTCTTC 2 cut(s) 161, 344
EarI CTCTTC 2 cut(s) 161, 344
Eco130I CCWWGG 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 46
EcoRI GAATTC 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 462
EcoT14I CCWWGG 1 cut(s) 130
ErhI CCWWGG 1 cut(s) 130
FaeI CATG 5 cut(s) 134, 152, 182, 275, 346
FaiI YATR 9 cut(s) 74, 87, 132, 150, 180, 273, 290, 316, 344
FatI CATG 5 cut(s) 130, 148, 178, 271, 342
FbaI TGATCA 1 cut(s) 414
Fnu4HI GCNGC 2 cut(s) 428, 431
Fsp4HI GCNGC 2 cut(s) 428, 431
FspBI CTAG 1 cut(s) 599
GlaI GCGC 1 cut(s) 90
GluI GCNGC 2 cut(s) 428, 431
GsuI CTGGAG 1 cut(s) 455
HaeIII GGCC 1 cut(s) 270
HhaI GCGC 1 cut(s) 91
Hin1II CATG 5 cut(s) 134, 152, 182, 275, 346
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HindIII AAGCTT 2 cut(s) 366, 407
HinfI GANTC 1 cut(s) 547
HphI GGTGA 3 cut(s) 103, 214, 415
Hpy188I TCNGA 2 cut(s) 143, 538
Hpy188III TCNNGA 2 cut(s) 160, 449
HpyAV CCTTC 3 cut(s) 32, 260, 394
HpyCH4III ACNGT 2 cut(s) 100, 490
HpyCH4V TGCA 1 cut(s) 311
HpyF3I CTNAG 3 cut(s) 447, 495, 579
Hsp92II CATG 5 cut(s) 134, 152, 182, 275, 346
HspAI GCGC 1 cut(s) 89
Ksp22I TGATCA 1 cut(s) 414
Kzo9I GATC 3 cut(s) 184, 278, 414
LmnI GCTCC 1 cut(s) 305
LpnPI CCDG 9 cut(s) 145, 238, 419, 434, 435, 449, 476, 507, 554
Lsp1109I GCAGC 2 cut(s) 417, 439
MaeI CTAG 1 cut(s) 599
MaeIII GTNAC 2 cut(s) 52, 236
MalI GATC 3 cut(s) 186, 280, 416
MboI GATC 3 cut(s) 184, 278, 414
MboII GAAGA 4 cut(s) 148, 194, 361, 468
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 1 cut(s) 253
MluCI AATT 5 cut(s) 7, 80, 119, 135, 255
MlyI GAGTC 1 cut(s) 556
MnlI CCTC 8 cut(s) 25, 164, 224, 255, 345, 445, 477, 529
MroXI GAANNNNTTC 1 cut(s) 571
MspA1I CMGCKG 1 cut(s) 430
MspR9I CCNGG 1 cut(s) 464
MvaI CCWGG 1 cut(s) 464
NcoI CCATGG 1 cut(s) 130
NdeII GATC 3 cut(s) 184, 278, 414
NlaIII CATG 5 cut(s) 134, 152, 182, 275, 346
NmuCI GTSAC 2 cut(s) 52, 236
PdmI GAANNNNTTC 1 cut(s) 571
PflMI CCANNNNNTGG 1 cut(s) 131
PkrI GCNGC 2 cut(s) 429, 432
PleI GAGTC 1 cut(s) 555
PpsI GAGTC 1 cut(s) 555
Psp6I CCWGG 1 cut(s) 462
PspGI CCWGG 1 cut(s) 462
PspPI GGNCC 1 cut(s) 46
PstNI CAGNNNCTG 1 cut(s) 433
PsuI RGATCY 1 cut(s) 184
PvuII CAGCTG 1 cut(s) 430
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
SatI GCNGC 2 cut(s) 428, 431
Sau3AI GATC 3 cut(s) 184, 278, 414
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 1 cut(s) 556
ScrFI CCNGG 1 cut(s) 464
SduI GDGCHC 1 cut(s) 253
SetI ASST 9 cut(s) 156, 295, 356, 370, 380, 395, 405, 411, 432
SinI GGWCC 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 553
SmoI CTYRAG 1 cut(s) 553
Sse9I AATT 5 cut(s) 7, 80, 119, 135, 255
SspI AATATT 1 cut(s) 594
SspMI CTAG 1 cut(s) 599
StyD4I CCNGG 1 cut(s) 462
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 2 cut(s) 100, 490
TasI AATT 5 cut(s) 7, 80, 119, 135, 255
TscAI CASTG 2 cut(s) 253, 548
TseFI GTSAC 2 cut(s) 52, 236
TseI GCWGC 2 cut(s) 427, 430
Tsp45I GTSAC 2 cut(s) 52, 236
TspDTI ATGAA 2 cut(s) 195, 362
TspRI CASTG 2 cut(s) 253, 548
Van91I CCANNNNNTGG 1 cut(s) 131
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 3 cut(s) 80, 119, 135
XmnI GAANNNNTTC 1 cut(s) 571
XspI CTAG 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.