AT1G65350

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0019549
Physical Location & Seq
Reverse (-)
24272518 .. 24277275
4758 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65350.1

Sequence Viewer

Length: 960 bp
ATGCAGATTTTTGTCAAGACTTTGACAGGCAAAACCATCACTTTGGAGGTTGAAAGTTCTGATACTATTGACAATGTTAAGGCTAAGATCCAGGATAAGGAAGGAATCCCTCCGGATCAGCAGAGGCTTATCTTTGCCGGTAAGCAGCTTGAAGACGGTCGCACACTCGCTGATTACAACATCCAAAAGGAGTCCACCCTCCATTTGGTGCTTCGTCTCAGAGGTGGTATGCAGATATTTGTGAAGACCCTCACTGGAAAGACAATCACTTTGGAGGTTGAGAGCTCAGACACCATCGATAACGTGAAGGCTAAGATTCAGGACAAGGAAGGTATCCCACCAGACCAACAGAGACTCATCTTCGCTGGGAAACAGCTTGAGGATGGTCGCACACTTGCTGACAATATCCAGAAGGAGTCTACGCTTCACCTTGTGTTGCGTCTTCGTGGTGGTATGCAAATCTTTGTGAAAACTTTGACAGGCAAGACCATTACCTTGGAGGTTGAGAGCTCAGACACAATTGATAATGTCAAAGCTAAGATTCAGGATAAGGAATGGATCCCACCAGACCAACAGAGACTCATCTTTGCTGGTAAACAGCTCGAGGATGGAAGGACTTTGGCTGATTACAACATCCAGAAAGAGTCTACTCTTCACCTTGTATTGCGTCTTCGTGGTGGTATGCAAATCTTTGTGAAAACTTTGACAGGCAAGACAATTACCTTGGAGGTTGAGAGCTCAGGCACAATCGATAACGTCAAGGCTAAGATACAGGACAAGGAGGGGATTCCACCAGACCAACAGAGACTCATCTTCGCTGGGAAACAGCTCGAGGGAGGACCGGGGGGGGGGGGGGGACACTTCCAAAAGGCAGAAGCATTGGCTTTTTTGGCTGATTACAACATCCAGAAGGAATCGACTCTTCACTTGGTTCTTCGTCTTCGTGGTGGAAGCTTCTGA

Protein Analysis

319

Amino Acids

35.4

Weight (kDa)

7.82

Isoelectric Point (pI)

29.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 6.3e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 8e-32 Ubiquitin family
Rad60-SLD PF11976 77 - 146 9.9e-14 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 79 - 149 4.5e-28 Ubiquitin family
Rad60-SLD PF11976 152 - 222 6.9e-16 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 154 - 225 2.3e-30 Ubiquitin family
Rad60-SLD PF11976 228 - 278 1.6e-09 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 230 - 278 1.7e-18 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65350
fragaria_vesca FvH4_6g15622
prunus_persica Prupe.6G238900_v2.0.a1
pyrus_communis pycom05g31060
rosa_chinensis RchiOBHm_Chr3g0469291
rosa_samantha Rh5BG034500 Rh5CG290800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 419, 647
AccIII TCCGGA 1 cut(s) 112
AclWI GGATC 4 cut(s) 82, 123, 553, 566
AdeI CACNNNGTG 1 cut(s) 433
AfiI CCNNNNNNNGG 3 cut(s) 97, 205, 848
AgsI TTSAA 2 cut(s) 53, 152
AjnI CCWGG 1 cut(s) 90
AluBI AGCT 9 cut(s) 148, 285, 376, 510, 536, 601, 738, 829, 954
AluI AGCT 9 cut(s) 148, 285, 376, 510, 536, 601, 738, 829, 954
Alw21I GWGCWC 3 cut(s) 287, 512, 740
Alw26I GTCTC 4 cut(s) 221, 346, 571, 799
AlwI GGATC 4 cut(s) 82, 123, 553, 566
Ama87I CYCGRG 2 cut(s) 602, 830
Aor13HI TCCGGA 1 cut(s) 112
ApeKI GCWGC 1 cut(s) 145
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
AspS9I GGNCC 1 cut(s) 839
AsuC2I CCSGG 1 cut(s) 843
AsuHPI GGTGA 2 cut(s) 419, 647
AvaI CYCGRG 2 cut(s) 602, 830
AvaII GGWCC 1 cut(s) 839
BamHI GGATCC 1 cut(s) 558
BanII GRGCYC 3 cut(s) 287, 512, 740
BarI GAAGNNNNNNTAC 2 cut(s) 654, 686
BbsI GAAGAC 5 cut(s) 159, 251, 434, 662, 932
Bbv12I GWGCWC 3 cut(s) 287, 512, 740
BbvI GCAGC 1 cut(s) 157
BccI CCATC 4 cut(s) 44, 302, 377, 602
BciT130I CCWGG 1 cut(s) 92
BciVI GTATCC 1 cut(s) 344
BcnI CCSGG 1 cut(s) 843
BcoDI GTCTC 4 cut(s) 221, 346, 571, 799
BfuI GTATCC 1 cut(s) 344
BisI GCNGC 1 cut(s) 146
BlsI GCNGC 1 cut(s) 147
Bme1390I CCNGG 2 cut(s) 92, 843
Bme18I GGWCC 1 cut(s) 839
BmeT110I CYCGRG 2 cut(s) 602, 830
BmgT120I GGNCC 1 cut(s) 839
BmiI GGNNCC 1 cut(s) 560
BmrFI CCNGG 2 cut(s) 92, 843
BpiI GAAGAC 5 cut(s) 159, 251, 434, 662, 932
Bpu10I CCTNAGC 1 cut(s) 739
BpuEI CTTGAG 1 cut(s) 398
BpuMI CCSGG 1 cut(s) 843
Bsa29I ATCGAT 2 cut(s) 297, 750
BsaJI CCNNGG 3 cut(s) 495, 723, 842
BsaWI WCCGGW 1 cut(s) 112
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 205, 848
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 1 cut(s) 259
BseAI TCCGGA 1 cut(s) 112
BseBI CCWGG 1 cut(s) 92
BseCI ATCGAT 2 cut(s) 297, 750
BseDI CCNNGG 3 cut(s) 495, 723, 842
BseGI GGATG 5 cut(s) 180, 388, 613, 633, 903
BseLI CCNNNNNNNGG 3 cut(s) 97, 205, 848
BseMII CTCAG 4 cut(s) 232, 300, 525, 753
BseNI ACTGG 1 cut(s) 259
BseXI GCAGC 1 cut(s) 157
BseYI CCCAGC 2 cut(s) 365, 818
Bsh1285I CGRYCG 1 cut(s) 160
BshVI ATCGAT 2 cut(s) 297, 750
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 3 cut(s) 287, 512, 740
BsiHKCI CYCGRG 2 cut(s) 602, 830
BsiSI CCGG 3 cut(s) 113, 138, 842
BslFI GGGAC 1 cut(s) 870
BslI CCNNNNNNNGG 3 cut(s) 97, 205, 848
BsmAI GTCTC 4 cut(s) 221, 346, 571, 799
BsmBI CGTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 870
BsoBI CYCGRG 2 cut(s) 602, 830
Bsp1286I GDGCHC 3 cut(s) 287, 512, 740
Bsp13I TCCGGA 1 cut(s) 112
Bsp143I GATC 3 cut(s) 87, 115, 558
BspCNI CTCAG 4 cut(s) 231, 299, 524, 752
BspDI ATCGAT 2 cut(s) 297, 750
BspEI TCCGGA 1 cut(s) 112
BspLI GGNNCC 1 cut(s) 560
BspPI GGATC 4 cut(s) 82, 123, 553, 566
BsrFI RCCGGY 1 cut(s) 137
BsrI ACTGG 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 3 cut(s) 495, 723, 842
BssMI GATC 3 cut(s) 87, 115, 558
BssT1I CCWWGG 2 cut(s) 495, 723
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 1 cut(s) 158
Bst6I CTCTTC 2 cut(s) 657, 927
BstDEI CTNAG 8 cut(s) 84, 218, 286, 312, 511, 537, 739, 765
BstF5I GGATG 5 cut(s) 180, 388, 613, 633, 903
BstKTI GATC 3 cut(s) 90, 118, 561
BstMAI GTCTC 4 cut(s) 221, 346, 571, 799
BstMBI GATC 3 cut(s) 87, 115, 558
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 1 cut(s) 890
BstNI CCWGG 1 cut(s) 92
BstSCI CCNGG 2 cut(s) 90, 841
BstV1I GCAGC 1 cut(s) 157
BstV2I GAAGAC 5 cut(s) 159, 251, 434, 662, 932
BstX2I RGATCY 2 cut(s) 87, 558
BstXI CCANNNNNNTGG 2 cut(s) 43, 496
BstYI RGATCY 2 cut(s) 87, 558
Bsu15I ATCGAT 2 cut(s) 297, 750
BsuI GTATCC 1 cut(s) 344
BsuTUI ATCGAT 2 cut(s) 297, 750
BtsCI GGATG 5 cut(s) 180, 388, 613, 633, 903
BtsIMutI CAGTG 1 cut(s) 252
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 1 cut(s) 839
ClaI ATCGAT 2 cut(s) 297, 750
CseI GACGC 2 cut(s) 428, 656
DdeI CTNAG 8 cut(s) 84, 218, 286, 312, 511, 537, 739, 765
DpnI GATC 3 cut(s) 89, 117, 560
DpnII GATC 3 cut(s) 87, 115, 558
DraIII CACNNNGTG 1 cut(s) 433
Eam1104I CTCTTC 2 cut(s) 657, 927
EarI CTCTTC 2 cut(s) 657, 927
Ecl136II GAGCTC 3 cut(s) 285, 510, 738
Eco130I CCWWGG 2 cut(s) 495, 723
Eco24I GRGCYC 3 cut(s) 287, 512, 740
Eco47I GGWCC 1 cut(s) 839
Eco53kI GAGCTC 3 cut(s) 285, 510, 738
Eco88I CYCGRG 2 cut(s) 602, 830
EcoICRI GAGCTC 3 cut(s) 285, 510, 738
EcoRII CCWGG 1 cut(s) 90
EcoT14I CCWWGG 2 cut(s) 495, 723
EcoT38I GRGCYC 3 cut(s) 287, 512, 740
ErhI CCWWGG 2 cut(s) 495, 723
Esp3I CGTCTC 1 cut(s) 221
FaiI YATR 3 cut(s) 230, 455, 683
FaqI GGGAC 1 cut(s) 870
FblI GTMKAC 2 cut(s) 419, 647
Fnu4HI GCNGC 1 cut(s) 146
FokI GGATG 5 cut(s) 167, 395, 620, 620, 890
FriOI GRGCYC 3 cut(s) 287, 512, 740
Fsp4HI GCNGC 1 cut(s) 146
GluI GCNGC 1 cut(s) 146
GsaI CCCAGC 2 cut(s) 369, 822
HapII CCGG 3 cut(s) 113, 138, 842
HgaI GACGC 2 cut(s) 428, 656
HindIII AAGCTT 1 cut(s) 952
HpaII CCGG 3 cut(s) 113, 138, 842
HphI GGTGA 2 cut(s) 419, 647
Hpy166II GTNNAC 4 cut(s) 195, 420, 596, 648
Hpy188I TCNGA 5 cut(s) 61, 221, 289, 514, 959
Hpy188III TCNNGA 7 cut(s) 16, 113, 320, 409, 545, 637, 907
Hpy8I GTNNAC 4 cut(s) 195, 420, 596, 648
HpyAV CCTTC 6 cut(s) 95, 301, 323, 406, 606, 904
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4IV ACGT 2 cut(s) 303, 756
HpyCH4V TGCA 4 cut(s) 4, 232, 457, 685
HpyF10VI GCNNNNNNNGC 1 cut(s) 890
HpyF3I CTNAG 8 cut(s) 84, 218, 286, 312, 511, 537, 739, 765
HpySE526I ACGT 2 cut(s) 303, 756
Kpn2I TCCGGA 1 cut(s) 112
Kzo9I GATC 3 cut(s) 87, 115, 558
Lsp1109I GCAGC 1 cut(s) 157
MaeII ACGT 2 cut(s) 303, 756
MalI GATC 3 cut(s) 89, 117, 560
MboI GATC 3 cut(s) 87, 115, 558
MfeI CAATTG 1 cut(s) 519
MflI RGATCY 2 cut(s) 87, 558
MhlI GDGCHC 3 cut(s) 287, 512, 740
MluCI AATT 2 cut(s) 519, 717
MlyI GAGTC 7 cut(s) 200, 348, 425, 573, 653, 801, 913
MroI TCCGGA 1 cut(s) 112
MseI TTAA 1 cut(s) 78
MspI CCGG 3 cut(s) 113, 138, 842
MspR9I CCNGG 2 cut(s) 92, 843
MunI CAATTG 1 cut(s) 519
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 1 cut(s) 890
NciI CCSGG 1 cut(s) 843
NdeII GATC 3 cut(s) 87, 115, 558
NlaIV GGNNCC 1 cut(s) 560
PaeR7I CTCGAG 2 cut(s) 602, 830
PfeI GAWTC 5 cut(s) 105, 316, 541, 787, 914
PfoI TCCNGGA 1 cut(s) 90
PkrI GCNGC 1 cut(s) 147
PleI GAGTC 7 cut(s) 199, 348, 424, 573, 652, 801, 913
PpsI GAGTC 7 cut(s) 199, 348, 424, 573, 652, 801, 913
Psp124BI GAGCTC 3 cut(s) 287, 512, 740
Psp6I CCWGG 1 cut(s) 90
PspFI CCCAGC 2 cut(s) 365, 818
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 1 cut(s) 560
PspPI GGNCC 1 cut(s) 839
PspXI VCTCGAGB 2 cut(s) 602, 830
PsuI RGATCY 2 cut(s) 87, 558
SacI GAGCTC 3 cut(s) 287, 512, 740
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 1 cut(s) 146
Sau3AI GATC 3 cut(s) 87, 115, 558
Sau96I GGNCC 1 cut(s) 839
SchI GAGTC 7 cut(s) 200, 348, 425, 573, 653, 801, 913
ScrFI CCNGG 2 cut(s) 92, 843
SduI GDGCHC 3 cut(s) 287, 512, 740
Sfr274I CTCGAG 2 cut(s) 602, 830
SinI GGWCC 1 cut(s) 839
SlaI CTCGAG 2 cut(s) 602, 830
SmlI CTYRAG 3 cut(s) 377, 602, 830
SmoI CTYRAG 3 cut(s) 377, 602, 830
Sse9I AATT 2 cut(s) 519, 717
SstI GAGCTC 3 cut(s) 287, 512, 740
StyD4I CCNGG 2 cut(s) 90, 841
StyI CCWWGG 2 cut(s) 495, 723
TaaI ACNGT 1 cut(s) 158
TaiI ACGT 2 cut(s) 306, 759
TaqI TCGA 5 cut(s) 297, 603, 750, 831, 917
TasI AATT 2 cut(s) 519, 717
TfiI GAWTC 5 cut(s) 105, 316, 541, 787, 914
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TscAI CASTG 1 cut(s) 259
TseI GCWGC 1 cut(s) 145
TspRI CASTG 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 839
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XhoI CTCGAG 2 cut(s) 602, 830
XmiI GTMKAC 2 cut(s) 419, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.