pycom05g31060

Ubiquitin-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0019549
Physical Location & Seq
Forward (+)
30946917 .. 30948083
1167 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g31060.1

Sequence Viewer

Length: 1167 bp
ATGATATTTTGGTTTCTGCAGATGCAAATCTTCGTGAAAACCCTTACTGGAAAGACCATCACCCTCGAGGTTGAGTCTTCCGACACCATCGACAACGTCAAAGCCAAGATCCAGGACAAGGAAGGGATCCCCCCAGACCAGCAGCGTCTTATCTTCGCCGGAAAGCAACTCGAAGACGGCCGAACCCTCGCAGACTACAACATCCAGAAGGAGTCAACCCTCCACTTGGTGCTCCGTCTGCGTGGTGGCATGCAGATCTTTGTCAAGACCCTCACCGGCAAGACTATCACCCTGGAGGTCGAAAGCTCCGACACCATCGACAACGTCAAGGCCAAGATCCAGGACAAGGAGGGCATCCCTCCAGACCAGCAGAGATTGATCTTTGCCGGAAAGCAGCTAGAGGATGGCAGGACCCTTGCCGACTACAACATCCAGAAGGAGTCCACCCTTCACTTGGTTCTTCGTCTCCGTGGTGGGATGCAAATCTTCGTCAAGACCCTTACAGGGAAGACCATCACCCTTGAGGTGGAGAGCTCCGACACCATCGACAACGTGAAGGCCAAGATTCAAGACAAGGAGGGCATCCCACCGGACCAGCAGAGGCTTATCTTTGCAGGAAAGCAGCTCGAGGACGGCCGCACCCTTGCCGACTACAACATCCAGAAAGAGTCCACTCTCCATTTGGTGCTTCGTCTCCGTGGTGGCATGCAGATTTTTGTGAAGACCCTGACTGGCAAGACCATCACCCTGGAGGTTGAGAGCTCGGACACCATCGACAATGTTAAGGCCAAGATCCAGGACAAGGAGGGGATCCCACCGGACCAGCAGAGGCTGATCTTTGCCGGGAAGCAACTCGAGGATGGCCGCACTTTGGCGGACTACAACATCCAGAAGGAGTCGACCCTTCACTTGGTGCTGCGTCTGAGGGGTGGTATGCAGATCTTTGTGAAGACCTTGACAGGGAAGACCATCACCTTGGAGGTGGAGAGCTCGGACACCATCGACAATGTGAAGGCTAAGATCCAGGACAAGGAGGGTATTCCTCCGGACCAGCAGAGGTTGATCTTCGCTGGTAAGCAGTTGGAGGATGGAAGGACCCTGGCTGATTATAACATTCAGAAGGAGTCCACCCTTCACCTTGTCCTCCGTCTCCGTGGTGGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

389

Amino Acids

43.66

Weight (kDa)

7.0

Isoelectric Point (pI)

29.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 8 - 78 8.7e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 10 - 81 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 84 - 154 8.7e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 86 - 157 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 160 - 230 8.7e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 162 - 233 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 236 - 306 8.7e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 238 - 309 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 312 - 382 8.7e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 314 - 385 1.1e-31 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65350
fragaria_vesca FvH4_6g15622
prunus_persica Prupe.6G238900_v2.0.a1
pyrus_communis pycom05g31060
rosa_chinensis RchiOBHm_Chr3g0469291
rosa_samantha Rh5BG034500 Rh5CG290800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1110
AbsI CCTCGAGG 1 cut(s) 65
AccI GTMKAC 1 cut(s) 899
AccIII TCCGGA 1 cut(s) 1045
AciI CCGC 3 cut(s) 637, 865, 875
AclWI GGATC 8 cut(s) 103, 121, 134, 331, 787, 805, 818, 1015
AcoI YGGCCR 3 cut(s) 178, 634, 862
AdeI CACNNNGTG 2 cut(s) 229, 913
AgsI TTSAA 1 cut(s) 569
AjnI CCWGG 7 cut(s) 111, 291, 339, 747, 795, 1023, 1098
AluBI AGCT 6 cut(s) 306, 397, 534, 625, 762, 990
AluI AGCT 6 cut(s) 306, 397, 534, 625, 762, 990
Alw21I GWGCWC 4 cut(s) 234, 536, 764, 992
Alw26I GTCTC 3 cut(s) 470, 698, 1154
AlwI GGATC 8 cut(s) 103, 121, 134, 331, 787, 805, 818, 1015
AlwNI CAGNNNCTG 1 cut(s) 832
Ama87I CYCGRG 3 cut(s) 65, 626, 854
Aor13HI TCCGGA 1 cut(s) 1045
AoxI GGCC 6 cut(s) 178, 330, 558, 634, 786, 862
ApeKI GCWGC 4 cut(s) 142, 394, 622, 916
AspS9I GGNCC 5 cut(s) 411, 592, 820, 1048, 1095
AsuC2I CCSGG 1 cut(s) 844
AsuHPI GGTGA 7 cut(s) 52, 265, 280, 508, 736, 964, 1127
AvaI CYCGRG 3 cut(s) 65, 626, 854
AvaII GGWCC 5 cut(s) 411, 592, 820, 1048, 1095
BamHI GGATCC 2 cut(s) 126, 810
BanII GRGCYC 3 cut(s) 536, 764, 992
BbsI GAAGAC 6 cut(s) 69, 180, 515, 728, 956, 971
Bbv12I GWGCWC 4 cut(s) 234, 536, 764, 992
BbvI GCAGC 4 cut(s) 154, 406, 634, 903
BceAI ACGGC 2 cut(s) 193, 649
BciT130I CCWGG 7 cut(s) 113, 293, 341, 749, 797, 1025, 1100
BcnI CCSGG 1 cut(s) 844
BcoDI GTCTC 3 cut(s) 470, 698, 1154
BfaI CTAG 2 cut(s) 398, 1165
BfmI CTRYAG 1 cut(s) 17
BglII AGATCT 2 cut(s) 255, 939
BisI GCNGC 6 cut(s) 143, 395, 623, 637, 865, 917
BlsI GCNGC 6 cut(s) 144, 396, 624, 638, 866, 918
Bme1390I CCNGG 8 cut(s) 113, 293, 341, 749, 797, 844, 1025, 1100
Bme18I GGWCC 5 cut(s) 411, 592, 820, 1048, 1095
BmeT110I CYCGRG 3 cut(s) 65, 626, 854
BmgT120I GGNCC 5 cut(s) 411, 592, 820, 1048, 1095
BmiI GGNNCC 4 cut(s) 128, 413, 812, 1097
BmrFI CCNGG 8 cut(s) 113, 293, 341, 749, 797, 844, 1025, 1100
BmsI GCATC 4 cut(s) 12, 363, 468, 591
BpiI GAAGAC 6 cut(s) 69, 180, 515, 728, 956, 971
BpmI CTGGAG 3 cut(s) 314, 345, 770
BpuEI CTTGAG 1 cut(s) 542
BpuMI CCSGG 1 cut(s) 844
BsaBI GATNNNNATC 2 cut(s) 26, 482
BsaJI CCNNGG 7 cut(s) 291, 469, 697, 747, 975, 1098, 1153
BsaWI WCCGGW 3 cut(s) 589, 817, 1045
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 2 cut(s) 52, 736
Bse8I GATNNNNATC 2 cut(s) 26, 482
BseAI TCCGGA 1 cut(s) 1045
BseBI CCWGG 7 cut(s) 113, 293, 341, 749, 797, 1025, 1100
BseDI CCNNGG 7 cut(s) 291, 469, 697, 747, 975, 1098, 1153
BseJI GATNNNNATC 2 cut(s) 26, 482
BseMII CTCAG 1 cut(s) 914
BseNI ACTGG 2 cut(s) 52, 736
BseX3I CGGCCG 2 cut(s) 178, 634
BseXI GCAGC 4 cut(s) 154, 406, 634, 903
Bsh1285I CGRYCG 2 cut(s) 181, 637
BshFI GGCC 6 cut(s) 180, 332, 560, 636, 788, 864
BsiEI CGRYCG 2 cut(s) 181, 637
BsiHKAI GWGCWC 4 cut(s) 234, 536, 764, 992
BsiHKCI CYCGRG 3 cut(s) 65, 626, 854
BsiSI CCGG 7 cut(s) 159, 276, 387, 590, 818, 843, 1046
BsmAI GTCTC 3 cut(s) 470, 698, 1154
BsmBI CGTCTC 3 cut(s) 470, 698, 1154
BsnI GGCC 6 cut(s) 180, 332, 560, 636, 788, 864
BsoBI CYCGRG 3 cut(s) 65, 626, 854
Bsp1286I GDGCHC 4 cut(s) 234, 536, 764, 992
Bsp13I TCCGGA 1 cut(s) 1045
BspACI CCGC 3 cut(s) 637, 865, 875
BspANI GGCC 6 cut(s) 180, 332, 560, 636, 788, 864
BspCNI CTCAG 1 cut(s) 915
BspEI TCCGGA 1 cut(s) 1045
BspLI GGNNCC 4 cut(s) 128, 413, 812, 1097
BspMAI CTGCAG 1 cut(s) 21
BspPI GGATC 8 cut(s) 103, 121, 134, 331, 787, 805, 818, 1015
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 2 cut(s) 52, 736
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 7 cut(s) 291, 469, 697, 747, 975, 1098, 1153
BssT1I CCWWGG 1 cut(s) 975
Bst2UI CCWGG 7 cut(s) 113, 293, 341, 749, 797, 1025, 1100
BstC8I GCNNGC 2 cut(s) 251, 707
BstDEI CTNAG 2 cut(s) 923, 1017
BstDSI CCRYGG 3 cut(s) 469, 697, 1153
BstMAI GTCTC 3 cut(s) 470, 698, 1154
BstMCI CGRYCG 2 cut(s) 181, 637
BstMWI GCNNNNNNNGC 1 cut(s) 238
BstNI CCWGG 7 cut(s) 113, 293, 341, 749, 797, 1025, 1100
BstNSI RCATGY 2 cut(s) 253, 709
BstSCI CCNGG 8 cut(s) 111, 291, 339, 747, 795, 842, 1023, 1098
BstSFI CTRYAG 1 cut(s) 17
BstV1I GCAGC 4 cut(s) 154, 406, 634, 903
BstV2I GAAGAC 6 cut(s) 69, 180, 515, 728, 956, 971
BstX2I RGATCY 8 cut(s) 108, 126, 255, 336, 792, 810, 939, 1020
BstXI CCANNNNNNTGG 2 cut(s) 748, 976
BstYI RGATCY 8 cut(s) 108, 126, 255, 336, 792, 810, 939, 1020
BstZI CGGCCG 2 cut(s) 178, 634
BsuRI GGCC 6 cut(s) 180, 332, 560, 636, 788, 864
BtgI CCRYGG 3 cut(s) 469, 697, 1153
Cac8I GCNNGC 2 cut(s) 251, 707
CaiI CAGNNNCTG 1 cut(s) 832
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 5 cut(s) 411, 592, 820, 1048, 1095
CseI GACGC 2 cut(s) 134, 908
CviAII CATG 2 cut(s) 250, 706
DdeI CTNAG 2 cut(s) 923, 1017
DraIII CACNNNGTG 2 cut(s) 229, 913
EaeI YGGCCR 3 cut(s) 178, 634, 862
EagI CGGCCG 2 cut(s) 178, 634
EciI GGCGGA 1 cut(s) 890
Ecl136II GAGCTC 3 cut(s) 534, 762, 990
EclXI CGGCCG 2 cut(s) 178, 634
Eco130I CCWWGG 1 cut(s) 975
Eco24I GRGCYC 3 cut(s) 536, 764, 992
Eco47I GGWCC 5 cut(s) 411, 592, 820, 1048, 1095
Eco52I CGGCCG 2 cut(s) 178, 634
Eco53kI GAGCTC 3 cut(s) 534, 762, 990
Eco88I CYCGRG 3 cut(s) 65, 626, 854
EcoICRI GAGCTC 3 cut(s) 534, 762, 990
EcoO109I RGGNCCY 2 cut(s) 411, 1095
EcoRII CCWGG 7 cut(s) 111, 291, 339, 747, 795, 1023, 1098
EcoT14I CCWWGG 1 cut(s) 975
EcoT38I GRGCYC 3 cut(s) 536, 764, 992
ErhI CCWWGG 1 cut(s) 975
Esp3I CGTCTC 3 cut(s) 470, 698, 1154
FaeI CATG 2 cut(s) 253, 709
FaiI YATR 4 cut(s) 251, 707, 935, 1110
FatI CATG 2 cut(s) 249, 705
FblI GTMKAC 1 cut(s) 899
Fnu4HI GCNGC 6 cut(s) 143, 395, 623, 637, 865, 917
FriOI GRGCYC 3 cut(s) 536, 764, 992
Fsp4HI GCNGC 6 cut(s) 143, 395, 623, 637, 865, 917
FspBI CTAG 2 cut(s) 398, 1165
GluI GCNGC 6 cut(s) 143, 395, 623, 637, 865, 917
GsuI CTGGAG 3 cut(s) 314, 345, 770
HaeIII GGCC 6 cut(s) 180, 332, 560, 636, 788, 864
HapII CCGG 7 cut(s) 159, 276, 387, 590, 818, 843, 1046
HgaI GACGC 2 cut(s) 134, 908
Hin1II CATG 2 cut(s) 253, 709
HincII GTYRAC 2 cut(s) 216, 900
HindII GTYRAC 2 cut(s) 216, 900
HinfI GANTC 7 cut(s) 74, 212, 440, 565, 668, 896, 1124
HpaII CCGG 7 cut(s) 159, 276, 387, 590, 818, 843, 1046
HphI GGTGA 7 cut(s) 52, 265, 280, 508, 736, 964, 1127
Hpy166II GTNNAC 5 cut(s) 216, 444, 672, 900, 1128
Hpy188I TCNGA 7 cut(s) 82, 310, 538, 766, 924, 994, 1119
Hpy8I GTNNAC 5 cut(s) 216, 444, 672, 900, 1128
HpyCH4IV ACGT 3 cut(s) 96, 324, 552
HpyCH4V TGCA 7 cut(s) 19, 25, 253, 481, 614, 709, 937
HpyF10VI GCNNNNNNNGC 1 cut(s) 238
HpyF3I CTNAG 2 cut(s) 923, 1017
HpySE526I ACGT 3 cut(s) 96, 324, 552
Hsp92II CATG 2 cut(s) 253, 709
Kpn2I TCCGGA 1 cut(s) 1045
LmnI GCTCC 3 cut(s) 237, 311, 539
Lsp1109I GCAGC 4 cut(s) 154, 406, 634, 903
LweI GCATC 4 cut(s) 12, 363, 468, 591
MaeI CTAG 2 cut(s) 398, 1165
MaeII ACGT 3 cut(s) 96, 324, 552
MflI RGATCY 8 cut(s) 108, 126, 255, 336, 792, 810, 939, 1020
MhlI GDGCHC 4 cut(s) 234, 536, 764, 992
MlyI GAGTC 6 cut(s) 83, 221, 449, 677, 905, 1133
MmeI TCCRAC 4 cut(s) 105, 333, 561, 1062
MroI TCCGGA 1 cut(s) 1045
MseI TTAA 1 cut(s) 783
MspI CCGG 7 cut(s) 159, 276, 387, 590, 818, 843, 1046
MspR9I CCNGG 8 cut(s) 113, 293, 341, 749, 797, 844, 1025, 1100
MvaI CCWGG 7 cut(s) 113, 293, 341, 749, 797, 1025, 1100
MwoI GCNNNNNNNGC 1 cut(s) 238
NciI CCSGG 1 cut(s) 844
NlaIII CATG 2 cut(s) 253, 709
NlaIV GGNNCC 4 cut(s) 128, 413, 812, 1097
NspI RCATGY 2 cut(s) 253, 709
PaeI GCATGC 2 cut(s) 253, 709
PaeR7I CTCGAG 3 cut(s) 65, 626, 854
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PfeI GAWTC 1 cut(s) 565
PflFI GACNNNGTC 2 cut(s) 95, 323
PfoI TCCNGGA 4 cut(s) 111, 339, 795, 1023
PkrI GCNGC 6 cut(s) 144, 396, 624, 638, 866, 918
PleI GAGTC 6 cut(s) 82, 220, 448, 676, 904, 1132
PpsI GAGTC 6 cut(s) 82, 220, 448, 676, 904, 1132
PpuMI RGGWCCY 2 cut(s) 411, 1095
PsiI TTATAA 1 cut(s) 1110
Psp124BI GAGCTC 3 cut(s) 536, 764, 992
Psp5II RGGWCCY 2 cut(s) 411, 1095
Psp6I CCWGG 7 cut(s) 111, 291, 339, 747, 795, 1023, 1098
PspGI CCWGG 7 cut(s) 111, 291, 339, 747, 795, 1023, 1098
PspN4I GGNNCC 4 cut(s) 128, 413, 812, 1097
PspPI GGNCC 5 cut(s) 411, 592, 820, 1048, 1095
PspPPI RGGWCCY 2 cut(s) 411, 1095
PspXI VCTCGAGB 3 cut(s) 65, 626, 854
PstI CTGCAG 1 cut(s) 21
PstNI CAGNNNCTG 1 cut(s) 832
PsuI RGATCY 8 cut(s) 108, 126, 255, 336, 792, 810, 939, 1020
PsyI GACNNNGTC 2 cut(s) 95, 323
SacI GAGCTC 3 cut(s) 536, 764, 992
SalI GTCGAC 1 cut(s) 898
SaqAI TTAA 1 cut(s) 783
SatI GCNGC 6 cut(s) 143, 395, 623, 637, 865, 917
Sau96I GGNCC 5 cut(s) 411, 592, 820, 1048, 1095
SchI GAGTC 6 cut(s) 83, 221, 449, 677, 905, 1133
ScrFI CCNGG 8 cut(s) 113, 293, 341, 749, 797, 844, 1025, 1100
SduI GDGCHC 4 cut(s) 234, 536, 764, 992
SfaNI GCATC 4 cut(s) 12, 363, 468, 591
SfcI CTRYAG 1 cut(s) 17
Sfr274I CTCGAG 3 cut(s) 65, 626, 854
SinI GGWCC 5 cut(s) 411, 592, 820, 1048, 1095
SlaI CTCGAG 3 cut(s) 65, 626, 854
SmlI CTYRAG 4 cut(s) 65, 521, 626, 854
SmoI CTYRAG 4 cut(s) 65, 521, 626, 854
SphI GCATGC 2 cut(s) 253, 709
SsiI CCGC 3 cut(s) 637, 865, 875
SspMI CTAG 2 cut(s) 398, 1165
SstI GAGCTC 3 cut(s) 536, 764, 992
StyD4I CCNGG 8 cut(s) 111, 291, 339, 747, 795, 842, 1023, 1098
StyI CCWWGG 1 cut(s) 975
TaiI ACGT 3 cut(s) 99, 327, 555
TauI GCSGC 2 cut(s) 639, 867
TfiI GAWTC 1 cut(s) 565
Tru1I TTAA 1 cut(s) 783
Tru9I TTAA 1 cut(s) 783
TseI GCWGC 4 cut(s) 142, 394, 622, 916
TspGWI ACGGA 5 cut(s) 224, 458, 686, 1136, 1142
Tth111I GACNNNGTC 2 cut(s) 95, 323
VpaK11BI GGWCC 5 cut(s) 411, 592, 820, 1048, 1095
XceI RCATGY 2 cut(s) 253, 709
XcmI CCANNNNNNNNNTGG 2 cut(s) 451, 679
XhoI CTCGAG 3 cut(s) 65, 626, 854
XmiI GTMKAC 1 cut(s) 899
XspI CTAG 2 cut(s) 398, 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.