FvH4_6g15622

Ubiquitin-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0019549
Physical Location & Seq
Reverse (-)
9728194 .. 9729114
921 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g15622.t1

Sequence Viewer

Length: 921 bp
ATGCAAATTTTTGTGAAAACCCTAACCGGCAAGACCATCACCCTCGAGGTCGAGAGCTCCGACACCGTCGACAACGTCAAGGCCAAGATCCAGGACAAGGAGGGCATCCCTCCTGACCAGCAGAGGCTCATCTTCGCCGGAAAGCAGCTTGAGGACGGCCGTACCCTCGCCGACTACAACATCCAGAAGGAGTCGACTCTCCACCTGGTGCTCCGTCTCAGGGGTGGTATGCAGATCTTCGTCAAAACCCTAACCGGGAAGACGATCACTCTCGAGGTCGAGAGCTCTGATACCATTGACAACGTCAAGGCCAAGATCCAGGACAAGGAGGGAATTCCCCCGGACCAGCAGAGGTTGATCTTCGCCGGTAAGCAGTTGGAGGATGGCCGAACTTTGGCCGATTACAATATCCAGAAGGAGTCCACCCTCCATTTGGTGCTCCGTCTTCGCGGTGGTATGCAAATCTTCGTGAAGACTCTCACCGGGAAGACAATCACTCTCGAAGTTGAGAGCTCCGACACGATTGATAACGTCAAGGCCAAGATCCAGGATAAGGAAGGGATCCCACCGGATCAGCAGAGGTTGATTTTCGCTGGTAAGCAATTGGAGGATGGCAGGACTCTTGCGGATTACAATATCCAGAAGGAGTCGACTCTGCATTTGGTGCTCAGGCTTCGTGGTGGGATGCAGATTTTTGTGAAGACTTTGACAGGCAAGACCATCACTTTGGAGGTTGAGAGCTCGGATACCATTGATAATGTCAAAGCCAAGATCCAGGATAAGGAAGGGATCCCACCGGACCAGCAAAGGTTGATCTTTGCCGGCAAGCAGCTCGAGGACGGTCGTACCCTGGCTGATTACAACATTCAGAAGGAGTCCACCCTTCACTTGGTCCTCCGTCTCCGTGGCGGTGACCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03320 map03320
Pfam Domains
Protein Families
ubiquitin

Protein Analysis

307

Amino Acids

34.26

Weight (kDa)

6.48

Isoelectric Point (pI)

28.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 3.3e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 5.1e-32 Ubiquitin family
Rad60-SLD PF11976 77 - 147 5.9e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 79 - 150 7.4e-32 Ubiquitin family
Rad60-SLD PF11976 153 - 223 5.9e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 155 - 226 7.4e-32 Ubiquitin family
Rad60-SLD PF11976 229 - 299 5.9e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 231 - 302 7.4e-32 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019549)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65350
fragaria_vesca FvH4_6g15622
prunus_persica Prupe.6G238900_v2.0.a1
pyrus_communis pycom05g31060
rosa_chinensis RchiOBHm_Chr3g0469291
rosa_samantha Rh5BG034500 Rh5CG290800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 44
AccI GTMKAC 3 cut(s) 69, 194, 650
AccII CGCG 1 cut(s) 450
AciI CCGC 3 cut(s) 450, 626, 909
AclWI GGATC 9 cut(s) 82, 310, 538, 556, 569, 579, 766, 784, 797
AcoI YGGCCR 3 cut(s) 157, 385, 396
AcsI RAATTY 2 cut(s) 6, 333
AdeI CACNNNGTG 1 cut(s) 208
AfaI GTAC 2 cut(s) 163, 847
AfiI CCNNNNNNNGG 9 cut(s) 97, 220, 255, 325, 394, 433, 553, 781, 889
AjnI CCWGG 6 cut(s) 90, 204, 318, 546, 774, 849
AluBI AGCT 6 cut(s) 57, 148, 285, 513, 741, 832
AluI AGCT 6 cut(s) 57, 148, 285, 513, 741, 832
Alw21I GWGCWC 7 cut(s) 59, 213, 287, 441, 515, 669, 743
Alw26I GTCTC 2 cut(s) 221, 905
AlwI GGATC 9 cut(s) 82, 310, 538, 556, 569, 579, 766, 784, 797
Ama87I CYCGRG 3 cut(s) 44, 272, 833
AoxI GGCC 6 cut(s) 81, 157, 309, 385, 396, 537
ApeKI GCWGC 2 cut(s) 145, 829
ApoI RAATTY 2 cut(s) 6, 333
ArsI GACNNNNNNTTYG 4 cut(s) 643, 675, 709, 741
AspS9I GGNCC 3 cut(s) 343, 799, 892
AsuC2I CCSGG 3 cut(s) 256, 341, 484
AsuHPI GGTGA 2 cut(s) 31, 472
AvaI CYCGRG 3 cut(s) 44, 272, 833
AvaII GGWCC 3 cut(s) 343, 799, 892
BaeI ACNNNNGTAYC 4 cut(s) 145, 178, 829, 862
BamHI GGATCC 2 cut(s) 561, 789
BanII GRGCYC 4 cut(s) 59, 287, 515, 743
BbsI GAAGAC 5 cut(s) 266, 437, 479, 494, 707
Bbv12I GWGCWC 7 cut(s) 59, 213, 287, 441, 515, 669, 743
BbvI GCAGC 2 cut(s) 157, 841
BccI CCATC 4 cut(s) 44, 377, 605, 728
BceAI ACGGC 2 cut(s) 144, 172
BcgI CGANNNNNNTGC 2 cut(s) 814, 848
BciT130I CCWGG 6 cut(s) 92, 206, 320, 548, 776, 851
BciVI GTATCC 1 cut(s) 739
BcnI CCSGG 3 cut(s) 256, 341, 484
BcoDI GTCTC 2 cut(s) 221, 905
BfuI GTATCC 1 cut(s) 739
BglII AGATCT 1 cut(s) 234
BisI GCNGC 2 cut(s) 146, 830
BlsI GCNGC 2 cut(s) 147, 831
Bme1390I CCNGG 9 cut(s) 92, 206, 256, 320, 341, 484, 548, 776, 851
Bme18I GGWCC 3 cut(s) 343, 799, 892
BmeT110I CYCGRG 3 cut(s) 44, 272, 833
BmgT120I GGNCC 3 cut(s) 343, 799, 892
BmiI GGNNCC 2 cut(s) 563, 791
BmrFI CCNGG 9 cut(s) 92, 206, 256, 320, 341, 484, 548, 776, 851
BmsI GCATC 2 cut(s) 114, 675
BpiI GAAGAC 5 cut(s) 266, 437, 479, 494, 707
Bpu10I CCTNAGC 1 cut(s) 668
BpuEI CTTGAG 1 cut(s) 170
BpuMI CCSGG 3 cut(s) 256, 341, 484
BsaJI CCNNGG 3 cut(s) 339, 849, 904
BsaWI WCCGGW 2 cut(s) 568, 796
Bsc4I CCNNNNNNNGG 9 cut(s) 97, 220, 255, 325, 394, 433, 553, 781, 889
Bse118I RCCGGY 3 cut(s) 26, 365, 821
BseBI CCWGG 6 cut(s) 92, 206, 320, 548, 776, 851
BseDI CCNNGG 3 cut(s) 339, 849, 904
BseGI GGATG 5 cut(s) 105, 180, 388, 616, 690
BseLI CCNNNNNNNGG 9 cut(s) 97, 220, 255, 325, 394, 433, 553, 781, 889
BseMII CTCAG 2 cut(s) 232, 682
BseX3I CGGCCG 1 cut(s) 157
BseXI GCAGC 2 cut(s) 157, 841
Bsh1236I CGCG 1 cut(s) 450
Bsh1285I CGRYCG 2 cut(s) 160, 844
BshFI GGCC 6 cut(s) 83, 159, 311, 387, 398, 539
BsiEI CGRYCG 2 cut(s) 160, 844
BsiHKAI GWGCWC 7 cut(s) 59, 213, 287, 441, 515, 669, 743
BsiHKCI CYCGRG 3 cut(s) 44, 272, 833
BsiSI CCGG 9 cut(s) 27, 138, 255, 341, 366, 483, 569, 797, 822
BslI CCNNNNNNNGG 9 cut(s) 97, 220, 255, 325, 394, 433, 553, 781, 889
BsmAI GTCTC 2 cut(s) 221, 905
BsmBI CGTCTC 2 cut(s) 221, 905
BsnI GGCC 6 cut(s) 83, 159, 311, 387, 398, 539
BsoBI CYCGRG 3 cut(s) 44, 272, 833
Bsp1286I GDGCHC 7 cut(s) 59, 213, 287, 441, 515, 669, 743
BspACI CCGC 3 cut(s) 450, 626, 909
BspANI GGCC 6 cut(s) 83, 159, 311, 387, 398, 539
BspCNI CTCAG 2 cut(s) 231, 681
BspFNI CGCG 1 cut(s) 450
BspLI GGNNCC 2 cut(s) 563, 791
BspPI GGATC 9 cut(s) 82, 310, 538, 556, 569, 579, 766, 784, 797
BsrFI RCCGGY 3 cut(s) 26, 365, 821
BssAI RCCGGY 3 cut(s) 26, 365, 821
BssECI CCNNGG 3 cut(s) 339, 849, 904
Bst2UI CCWGG 6 cut(s) 92, 206, 320, 548, 776, 851
Bst4CI ACNGT 2 cut(s) 67, 842
BstAPI GCANNNNNTGC 1 cut(s) 664
BstC8I GCNNGC 2 cut(s) 823, 827
BstDEI CTNAG 2 cut(s) 218, 668
BstDSI CCRYGG 1 cut(s) 904
BstEII GGTNACC 1 cut(s) 911
BstF5I GGATG 5 cut(s) 105, 180, 388, 616, 690
BstFNI CGCG 1 cut(s) 450
BstMAI GTCTC 2 cut(s) 221, 905
BstMCI CGRYCG 2 cut(s) 160, 844
BstMWI GCNNNNNNNGC 1 cut(s) 664
BstNI CCWGG 6 cut(s) 92, 206, 320, 548, 776, 851
BstPI GGTNACC 1 cut(s) 911
BstSCI CCNGG 9 cut(s) 90, 204, 254, 318, 339, 482, 546, 774, 849
BstUI CGCG 1 cut(s) 450
BstV1I GCAGC 2 cut(s) 157, 841
BstV2I GAAGAC 5 cut(s) 266, 437, 479, 494, 707
BstX2I RGATCY 7 cut(s) 87, 234, 315, 543, 561, 771, 789
BstXI CCANNNNNNTGG 1 cut(s) 727
BstYI RGATCY 7 cut(s) 87, 234, 315, 543, 561, 771, 789
BstZI CGGCCG 1 cut(s) 157
BsuI GTATCC 1 cut(s) 739
BsuRI GGCC 6 cut(s) 83, 159, 311, 387, 398, 539
BtgI CCRYGG 1 cut(s) 904
BtsCI GGATG 5 cut(s) 105, 180, 388, 616, 690
Cac8I GCNNGC 2 cut(s) 823, 827
Cfr10I RCCGGY 3 cut(s) 26, 365, 821
Cfr13I GGNCC 3 cut(s) 343, 799, 892
CsiI ACCWGGT 1 cut(s) 204
Csp6I GTAC 2 cut(s) 162, 846
CviQI GTAC 2 cut(s) 162, 846
DdeI CTNAG 2 cut(s) 218, 668
DraIII CACNNNGTG 1 cut(s) 208
EaeI YGGCCR 3 cut(s) 157, 385, 396
EagI CGGCCG 1 cut(s) 157
Ecl136II GAGCTC 4 cut(s) 57, 285, 513, 741
EclXI CGGCCG 1 cut(s) 157
Eco24I GRGCYC 4 cut(s) 59, 287, 515, 743
Eco47I GGWCC 3 cut(s) 343, 799, 892
Eco52I CGGCCG 1 cut(s) 157
Eco53kI GAGCTC 4 cut(s) 57, 285, 513, 741
Eco88I CYCGRG 3 cut(s) 44, 272, 833
Eco91I GGTNACC 1 cut(s) 911
EcoICRI GAGCTC 4 cut(s) 57, 285, 513, 741
EcoO65I GGTNACC 1 cut(s) 911
EcoRI GAATTC 1 cut(s) 333
EcoRII CCWGG 6 cut(s) 90, 204, 318, 546, 774, 849
EcoT38I GRGCYC 4 cut(s) 59, 287, 515, 743
Esp3I CGTCTC 2 cut(s) 221, 905
FaiI YATR 2 cut(s) 230, 458
FblI GTMKAC 3 cut(s) 69, 194, 650
Fnu4HI GCNGC 2 cut(s) 146, 830
FokI GGATG 5 cut(s) 92, 167, 395, 623, 697
FriOI GRGCYC 4 cut(s) 59, 287, 515, 743
Fsp4HI GCNGC 2 cut(s) 146, 830
GluI GCNGC 2 cut(s) 146, 830
HaeIII GGCC 6 cut(s) 83, 159, 311, 387, 398, 539
HapII CCGG 9 cut(s) 27, 138, 255, 341, 366, 483, 569, 797, 822
HincII GTYRAC 3 cut(s) 70, 195, 651
HindII GTYRAC 3 cut(s) 70, 195, 651
HinfI GANTC 8 cut(s) 191, 196, 419, 475, 619, 647, 652, 875
HpaII CCGG 9 cut(s) 27, 138, 255, 341, 366, 483, 569, 797, 822
HphI GGTGA 2 cut(s) 31, 472
Hpy166II GTNNAC 5 cut(s) 70, 195, 423, 651, 879
Hpy188I TCNGA 5 cut(s) 61, 289, 517, 745, 870
Hpy188III TCNNGA 9 cut(s) 52, 113, 184, 272, 280, 412, 469, 500, 640
Hpy8I GTNNAC 5 cut(s) 70, 195, 423, 651, 879
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 7 cut(s) 181, 409, 551, 637, 779, 865, 893
HpyCH4III ACNGT 2 cut(s) 67, 842
HpyCH4IV ACGT 3 cut(s) 75, 303, 531
HpyCH4V TGCA 5 cut(s) 4, 232, 460, 658, 688
HpyF10VI GCNNNNNNNGC 1 cut(s) 664
HpyF3I CTNAG 2 cut(s) 218, 668
HpySE526I ACGT 3 cut(s) 75, 303, 531
KroI GCCGGC 1 cut(s) 821
KroNI GCCGGC 1 cut(s) 823
LmnI GCTCC 4 cut(s) 62, 216, 444, 518
Lsp1109I GCAGC 2 cut(s) 157, 841
LweI GCATC 2 cut(s) 114, 675
MabI ACCWGGT 1 cut(s) 204
MaeII ACGT 3 cut(s) 75, 303, 531
MaeIII GTNAC 1 cut(s) 911
MboII GAAGA 9 cut(s) 124, 229, 271, 352, 437, 457, 484, 499, 712
MfeI CAATTG 1 cut(s) 602
MflI RGATCY 7 cut(s) 87, 234, 315, 543, 561, 771, 789
MhlI GDGCHC 7 cut(s) 59, 213, 287, 441, 515, 669, 743
MluCI AATT 3 cut(s) 6, 333, 602
MlyI GAGTC 8 cut(s) 190, 200, 428, 469, 613, 646, 656, 884
MmeI TCCRAC 3 cut(s) 84, 357, 540
MroNI GCCGGC 1 cut(s) 821
MseI TTAA 1 cut(s) 919
MspI CCGG 9 cut(s) 27, 138, 255, 341, 366, 483, 569, 797, 822
MspR9I CCNGG 9 cut(s) 92, 206, 256, 320, 341, 484, 548, 776, 851
MunI CAATTG 1 cut(s) 602
MvaI CCWGG 6 cut(s) 92, 206, 320, 548, 776, 851
MvnI CGCG 1 cut(s) 450
MwoI GCNNNNNNNGC 1 cut(s) 664
NaeI GCCGGC 1 cut(s) 823
NciI CCSGG 3 cut(s) 256, 341, 484
NgoMIV GCCGGC 1 cut(s) 821
NlaIV GGNNCC 2 cut(s) 563, 791
NmuCI GTSAC 1 cut(s) 911
PaeR7I CTCGAG 3 cut(s) 44, 272, 833
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PdiI GCCGGC 1 cut(s) 823
PflFI GACNNNGTC 3 cut(s) 65, 74, 302
PfoI TCCNGGA 4 cut(s) 90, 318, 546, 774
PkrI GCNGC 2 cut(s) 147, 831
PleI GAGTC 8 cut(s) 190, 199, 427, 469, 613, 646, 655, 883
PpsI GAGTC 8 cut(s) 190, 199, 427, 469, 613, 646, 655, 883
Psp124BI GAGCTC 4 cut(s) 59, 287, 515, 743
Psp6I CCWGG 6 cut(s) 90, 204, 318, 546, 774, 849
PspEI GGTNACC 1 cut(s) 911
PspGI CCWGG 6 cut(s) 90, 204, 318, 546, 774, 849
PspN4I GGNNCC 2 cut(s) 563, 791
PspPI GGNCC 3 cut(s) 343, 799, 892
PspXI VCTCGAGB 2 cut(s) 44, 833
PsuI RGATCY 7 cut(s) 87, 234, 315, 543, 561, 771, 789
PsyI GACNNNGTC 3 cut(s) 65, 74, 302
RsaI GTAC 2 cut(s) 163, 847
RsaNI GTAC 2 cut(s) 162, 846
SacI GAGCTC 4 cut(s) 59, 287, 515, 743
SalI GTCGAC 3 cut(s) 68, 193, 649
SaqAI TTAA 1 cut(s) 919
SatI GCNGC 2 cut(s) 146, 830
Sau96I GGNCC 3 cut(s) 343, 799, 892
SchI GAGTC 8 cut(s) 190, 200, 428, 469, 613, 646, 656, 884
ScrFI CCNGG 9 cut(s) 92, 206, 256, 320, 341, 484, 548, 776, 851
SduI GDGCHC 7 cut(s) 59, 213, 287, 441, 515, 669, 743
SexAI ACCWGGT 1 cut(s) 204
SfaNI GCATC 2 cut(s) 114, 675
Sfr274I CTCGAG 3 cut(s) 44, 272, 833
SinI GGWCC 3 cut(s) 343, 799, 892
SlaI CTCGAG 3 cut(s) 44, 272, 833
SmlI CTYRAG 4 cut(s) 44, 149, 272, 833
SmoI CTYRAG 4 cut(s) 44, 149, 272, 833
Sse9I AATT 3 cut(s) 6, 333, 602
SsiI CCGC 3 cut(s) 450, 626, 909
SstI GAGCTC 4 cut(s) 59, 287, 515, 743
StyD4I CCNGG 9 cut(s) 90, 204, 254, 318, 339, 482, 546, 774, 849
TaaI ACNGT 2 cut(s) 67, 842
TaiI ACGT 3 cut(s) 78, 306, 534
TaqI TCGA 9 cut(s) 45, 51, 69, 194, 273, 279, 501, 650, 834
TasI AATT 3 cut(s) 6, 333, 602
Tru1I TTAA 1 cut(s) 919
Tru9I TTAA 1 cut(s) 919
TseFI GTSAC 1 cut(s) 911
TseI GCWGC 2 cut(s) 145, 829
Tsp45I GTSAC 1 cut(s) 911
TspGWI ACGGA 4 cut(s) 203, 431, 887, 893
Tth111I GACNNNGTC 3 cut(s) 65, 74, 302
VpaK11BI GGWCC 3 cut(s) 343, 799, 892
XapI RAATTY 2 cut(s) 6, 333
XcmI CCANNNNNNNNNTGG 2 cut(s) 430, 886
XhoI CTCGAG 3 cut(s) 44, 272, 833
XmiI GTMKAC 3 cut(s) 69, 194, 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.