AT1G65390

Phloem protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0018741
Physical Location & Seq
Forward (+)
24292372 .. 24294740
2369 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65390.2

Sequence Viewer

Length: 1236 bp
ATGTCGGGAGCATCTTCTGTTTCTTCTATTTGTTCCTCTAATGTATCATTGATCCCTACGGGACCTCAAGTGTTCATCAATTTCCGGGGGAAAGATTTACGAAAAGGCTTCATGAGCTTTCTTAAGCCTGCTTTGAAAAAAGAAAAGATCAACGTGTTTATTGACGAACAAGAGGAGAGGGGCAAATACCTAATCAGTCTTTTTGATACGATAGGTGAGTCGAAAATCGCTTTGGTTATCTTTTCTGAAGGATACTGCGAGTCACATTGGTGTATGGACGAGCTGGTGAAAATCAAGGAATACATGGATCAAAACAGACTCATAATCATTCCAATATTCTACAGGCTTGATCTCGATGTGGTAAAAGATCTTACAGGAAAATTTGGCGATAATTTTTGGGATTTGGTAGATAAATATCAACCTGAACCAAAAAAGCTCCATAAATGGACGGAAGCTTTGTTCTCTGTTTGCGAATTGTTTTCCTTGATCTTACCAAAACACAGTGACATTTCGGATAGAGATTTTGTAAAGTCAATCGTCAAGGCTGTTAAAAAAGTGCAGAAGAATTTCTTCCAACGAAGAAATGGAGAAATAGAATATCAAGATTTCTCAGTCCCTGCATGTAAACTCACTATCACTATGCACGAGTCACCAAATGAGGAAGCCGTTCAAGTTACGGTGCTAAATGAATTTTACCAGATGAAGAATCAGTCACCAGTGCCCTCATACGAGTTCAAATTTTGGGTCGACCTTACGAGACCAAAGGGTAACGTTTTCATGATTGACGCAAGAGATCTTTCCATTGCATGGTCAGAGGACTCCAACCATTGGACCTGGCTCCCTCTCCCAAACCAAAATTCAAATGAATCAGTGATGGAAATTGCATTTTTGAAAAGTGCTAGCTGGCTAGACGTAGCTGGAAAGTTTGACACTCGATATTTGACCCCGAGGACAAGGTACGAGGTGGTGTTCGTAGTGAAGTTAGAATACACATTCGAATGGGAGACACTAGTGAAGCTAAAGCTAGACTTGCCGAATACTTGGGAGAAACCGCAAGAACAGAGTGTGGACATGTTTGACTACATAAGTGACCAATGGCTCGATATTCCAGTTGGAGAGTTCACCACATCGAAGAAAAACGTCGGGGAAATCTCTTTTGCAATGTACGAACACGAGTGCCAACTGTGGAAGTCAGGCCTCTTCGTCAAAGGTGTTACAATTCGTCCCAAATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2 TIR

Protein Analysis

411

Amino Acids

47.96

Weight (kDa)

5.6

Isoelectric Point (pI)

31.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 193 1.2e-34 TIR domain
TIR_2 PF13676 24 - 119 1.4e-11 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018741)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19960 AT1G65390 AT1G65390 AT1G65390 AT2G32140 AT5G45070 AT5G45080 AT5G45090 AT5G45090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 807, 828
AccI GTMKAC 1 cut(s) 747
AciI CCGC 1 cut(s) 1052
AclI AACGTT 1 cut(s) 771
AclWI GGATC 2 cut(s) 46, 315
AcsI RAATTY 5 cut(s) 380, 565, 689, 737, 856
AcuI CTGAAG 1 cut(s) 267
AfaI GTAC 2 cut(s) 959, 1166
AfiI CCNNNNNNNGG 2 cut(s) 807, 828
AflII CTTAAG 1 cut(s) 122
AflIII ACRYGT 2 cut(s) 153, 1071
AgsI TTSAA 5 cut(s) 136, 671, 736, 861, 892
AhlI ACTAGT 1 cut(s) 1009
AjnI CCWGG 1 cut(s) 833
AjuI GAANNNNNNNTTGG 4 cut(s) 215, 247, 841, 873
AleI CACNNNNGTG 1 cut(s) 268
AloI GAACNNNNNNTCC 2 cut(s) 443, 475
AluBI AGCT 8 cut(s) 117, 283, 436, 455, 903, 917, 1018, 1024
AluI AGCT 8 cut(s) 117, 283, 436, 455, 903, 917, 1018, 1024
Alw26I GTCTC 2 cut(s) 751, 998
AlwI GGATC 2 cut(s) 46, 315
AlwNI CAGNNNCTG 1 cut(s) 617
Ama87I CYCGRG 1 cut(s) 946
AoxI GGCC 1 cut(s) 1195
ApoI RAATTY 5 cut(s) 380, 565, 689, 737, 856
ArsI GACNNNNNNTTYG 4 cut(s) 439, 471, 729, 761
Asp700I GAANNNNTTC 3 cut(s) 566, 569, 666
AspS9I GGNCC 2 cut(s) 62, 831
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 5 cut(s) 227, 298, 642, 705, 1114
AsuII TTCGAA 1 cut(s) 996
AsuNHI GCTAGC 1 cut(s) 899
AvaI CYCGRG 1 cut(s) 946
AvaII GGWCC 2 cut(s) 62, 831
BaeGI GKGCMC 1 cut(s) 723
BarI GAAGNNNNNNTAC 2 cut(s) 971, 1003
BauI CACGAG 2 cut(s) 644, 1172
BccI CCATC 1 cut(s) 868
BceAI ACGGC 1 cut(s) 650
BciT130I CCWGG 1 cut(s) 835
BciVI GTATCC 1 cut(s) 245
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 2 cut(s) 751, 998
BcuI ACTAGT 1 cut(s) 1009
BfaI CTAG 4 cut(s) 900, 908, 1010, 1025
BfmI CTRYAG 1 cut(s) 340
BfrI CTTAAG 1 cut(s) 122
BfuI GTATCC 1 cut(s) 245
BglII AGATCT 2 cut(s) 367, 793
Bme1390I CCNGG 2 cut(s) 86, 835
Bme18I GGWCC 2 cut(s) 62, 831
BmeT110I CYCGRG 1 cut(s) 946
BmgT120I GGNCC 2 cut(s) 62, 831
BmiI GGNNCC 2 cut(s) 63, 839
BmrFI CCNGG 2 cut(s) 86, 835
BmsI GCATC 1 cut(s) 20
BmtI GCTAGC 1 cut(s) 903
Bpu14I TTCGAA 1 cut(s) 996
BpuEI CTTGAG 1 cut(s) 51
BpuMI CCSGG 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 414
BsaI GGTCTC 1 cut(s) 751
BsaJI CCNNGG 2 cut(s) 85, 947
Bsc4I CCNNNNNNNGG 2 cut(s) 807, 828
Bse1I ACTGG 2 cut(s) 716, 1109
Bse3DI GCAATG 2 cut(s) 801, 1167
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 1 cut(s) 835
BseDI CCNNGG 2 cut(s) 85, 947
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 2 cut(s) 807, 828
BseMI GCAATG 2 cut(s) 801, 1167
BseMII CTCAG 1 cut(s) 624
BseNI ACTGG 2 cut(s) 716, 1109
BseRI GAGGAG 1 cut(s) 188
BseSI GKGCMC 1 cut(s) 723
BsgI GTGCAG 1 cut(s) 578
BshFI GGCC 1 cut(s) 1197
BsiHKCI CYCGRG 1 cut(s) 946
BsiSI CCGG 1 cut(s) 85
BslFI GGGAC 3 cut(s) 75, 599, 1209
BslI CCNNNNNNNGG 2 cut(s) 807, 828
BsmAI GTCTC 2 cut(s) 751, 998
BsmFI GGGAC 3 cut(s) 75, 599, 1209
BsnI GGCC 1 cut(s) 1197
Bso31I GGTCTC 1 cut(s) 751
BsoBI CYCGRG 1 cut(s) 946
Bsp119I TTCGAA 1 cut(s) 996
Bsp1286I GDGCHC 1 cut(s) 723
Bsp143I GATC 7 cut(s) 51, 147, 307, 349, 367, 486, 793
BspACI CCGC 1 cut(s) 1052
BspANI GGCC 1 cut(s) 1197
BspCNI CTCAG 1 cut(s) 623
BspHI TCATGA 2 cut(s) 111, 777
BspLI GGNNCC 2 cut(s) 63, 839
BspOI GCTAGC 1 cut(s) 903
BspPI GGATC 2 cut(s) 46, 315
BspT104I TTCGAA 1 cut(s) 996
BspTI CTTAAG 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 751
BsrDI GCAATG 2 cut(s) 801, 1167
BsrI ACTGG 2 cut(s) 716, 1109
BssECI CCNNGG 2 cut(s) 85, 947
BssMI GATC 7 cut(s) 51, 147, 307, 349, 367, 486, 793
BssSI CACGAG 2 cut(s) 644, 1172
Bst2BI CACGAG 2 cut(s) 644, 1172
Bst2UI CCWGG 1 cut(s) 835
Bst4CI ACNGT 3 cut(s) 503, 679, 1185
Bst6I CTCTTC 1 cut(s) 1205
BstAFI CTTAAG 1 cut(s) 122
BstBI TTCGAA 1 cut(s) 996
BstC8I GCNNGC 3 cut(s) 129, 901, 905
BstDEI CTNAG 1 cut(s) 610
BstKTI GATC 7 cut(s) 54, 150, 310, 352, 370, 489, 796
BstMAI GTCTC 2 cut(s) 751, 998
BstMBI GATC 7 cut(s) 51, 147, 307, 349, 367, 486, 793
BstMWI GCNNNNNNNGC 2 cut(s) 114, 1030
BstNI CCWGG 1 cut(s) 835
BstNSI RCATGY 2 cut(s) 624, 1075
BstSCI CCNGG 2 cut(s) 84, 833
BstSFI CTRYAG 1 cut(s) 340
BstSLI GKGCMC 1 cut(s) 723
BstX2I RGATCY 2 cut(s) 367, 793
BstYI RGATCY 2 cut(s) 367, 793
BsuI GTATCC 1 cut(s) 245
BsuRI GGCC 1 cut(s) 1197
BtsIMutI CAGTG 3 cut(s) 508, 723, 876
Cac8I GCNNGC 3 cut(s) 129, 901, 905
CaiI CAGNNNCTG 1 cut(s) 617
CciI TCATGA 2 cut(s) 111, 777
Cfr13I GGNCC 2 cut(s) 62, 831
CseI GACGC 1 cut(s) 794
Csp6I GTAC 2 cut(s) 958, 1165
CviAII CATG 6 cut(s) 112, 304, 621, 778, 807, 1072
CviQI GTAC 2 cut(s) 958, 1165
DdeI CTNAG 1 cut(s) 610
DpnI GATC 7 cut(s) 53, 149, 309, 351, 369, 488, 795
DpnII GATC 7 cut(s) 51, 147, 307, 349, 367, 486, 793
Eam1104I CTCTTC 1 cut(s) 1205
EarI CTCTTC 1 cut(s) 1205
Eco147I AGGCCT 1 cut(s) 1197
Eco31I GGTCTC 1 cut(s) 751
Eco47I GGWCC 2 cut(s) 62, 831
Eco57I CTGAAG 1 cut(s) 267
Eco88I CYCGRG 1 cut(s) 946
EcoO109I RGGNCCY 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 833
FaeI CATG 6 cut(s) 115, 307, 624, 781, 810, 1075
FalI AAGNNNNNCTT 4 cut(s) 554, 586, 1013, 1045
FaqI GGGAC 3 cut(s) 75, 599, 1209
FatI CATG 6 cut(s) 111, 303, 620, 777, 806, 1071
FblI GTMKAC 1 cut(s) 747
FspBI CTAG 4 cut(s) 900, 908, 1010, 1025
HaeIII GGCC 1 cut(s) 1197
HapII CCGG 1 cut(s) 85
HgaI GACGC 1 cut(s) 794
Hin1II CATG 6 cut(s) 115, 307, 624, 781, 810, 1075
HincII GTYRAC 1 cut(s) 748
HindII GTYRAC 1 cut(s) 748
HindIII AAGCTT 1 cut(s) 453
HinfI GANTC 7 cut(s) 218, 260, 318, 647, 706, 818, 866
HpaII CCGG 1 cut(s) 85
HphI GGTGA 5 cut(s) 227, 298, 642, 705, 1114
Hpy166II GTNNAC 4 cut(s) 626, 748, 1069, 1122
Hpy188I TCNGA 3 cut(s) 247, 514, 814
Hpy188III TCNNGA 5 cut(s) 6, 112, 353, 602, 778
Hpy8I GTNNAC 4 cut(s) 626, 748, 1069, 1122
Hpy99I CGWCG 1 cut(s) 1145
HpyAV CCTTC 1 cut(s) 242
HpyCH4III ACNGT 3 cut(s) 503, 679, 1185
HpyCH4IV ACGT 4 cut(s) 153, 771, 912, 1140
HpyCH4V TGCA 6 cut(s) 559, 620, 643, 806, 884, 1160
HpyF10VI GCNNNNNNNGC 2 cut(s) 114, 1030
HpyF3I CTNAG 1 cut(s) 610
HpySE526I ACGT 4 cut(s) 153, 771, 912, 1140
Hsp92II CATG 6 cut(s) 115, 307, 624, 781, 810, 1075
Kzo9I GATC 7 cut(s) 51, 147, 307, 349, 367, 486, 793
LmnI GCTCC 3 cut(s) 8, 441, 843
LweI GCATC 1 cut(s) 20
MaeI CTAG 4 cut(s) 900, 908, 1010, 1025
MaeII ACGT 4 cut(s) 153, 771, 912, 1140
MaeIII GTNAC 8 cut(s) 261, 503, 648, 673, 711, 767, 1088, 1213
MalI GATC 7 cut(s) 53, 149, 309, 351, 369, 488, 795
MboI GATC 7 cut(s) 51, 147, 307, 349, 367, 486, 793
MboII GAAGA 8 cut(s) 6, 15, 562, 574, 591, 715, 1144, 1192
MflI RGATCY 2 cut(s) 367, 793
MhlI GDGCHC 1 cut(s) 723
MlyI GAGTC 5 cut(s) 227, 269, 312, 656, 812
MmeI TCCRAC 3 cut(s) 598, 846, 1093
MroXI GAANNNNTTC 3 cut(s) 566, 569, 666
MseI TTAA 2 cut(s) 123, 549
MslI CAYNNNNRTG 2 cut(s) 268, 997
MspCI CTTAAG 1 cut(s) 122
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 2 cut(s) 86, 835
MvaI CCWGG 1 cut(s) 835
MwoI GCNNNNNNNGC 2 cut(s) 114, 1030
NciI CCSGG 1 cut(s) 86
NdeII GATC 7 cut(s) 51, 147, 307, 349, 367, 486, 793
NheI GCTAGC 1 cut(s) 899
NlaIII CATG 6 cut(s) 115, 307, 624, 781, 810, 1075
NlaIV GGNNCC 2 cut(s) 63, 839
NmuCI GTSAC 5 cut(s) 261, 503, 648, 711, 1088
NspI RCATGY 2 cut(s) 624, 1075
NspV TTCGAA 1 cut(s) 996
OliI CACNNNNGTG 1 cut(s) 268
PagI TCATGA 2 cut(s) 111, 777
PceI AGGCCT 1 cut(s) 1197
PciI ACATGT 1 cut(s) 1071
PdmI GAANNNNTTC 3 cut(s) 566, 569, 666
PfeI GAWTC 2 cut(s) 706, 866
PflMI CCANNNNNTGG 2 cut(s) 807, 828
PleI GAGTC 5 cut(s) 226, 268, 312, 655, 812
PpsI GAGTC 5 cut(s) 226, 268, 312, 655, 812
PpuMI RGGWCCY 1 cut(s) 62
PscI ACATGT 1 cut(s) 1071
Psp1406I AACGTT 1 cut(s) 771
Psp5II RGGWCCY 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 833
PspGI CCWGG 1 cut(s) 833
PspN4I GGNNCC 2 cut(s) 63, 839
PspPI GGNCC 2 cut(s) 62, 831
PspPPI RGGWCCY 1 cut(s) 62
PstNI CAGNNNCTG 1 cut(s) 617
PsuI RGATCY 2 cut(s) 367, 793
RsaI GTAC 2 cut(s) 959, 1166
RsaNI GTAC 2 cut(s) 958, 1165
RseI CAYNNNNRTG 2 cut(s) 268, 997
SalI GTCGAC 1 cut(s) 746
SaqAI TTAA 2 cut(s) 123, 549
Sau3AI GATC 7 cut(s) 51, 147, 307, 349, 367, 486, 793
Sau96I GGNCC 2 cut(s) 62, 831
SchI GAGTC 5 cut(s) 227, 269, 312, 656, 812
ScrFI CCNGG 2 cut(s) 86, 835
SduI GDGCHC 1 cut(s) 723
SfaNI GCATC 1 cut(s) 20
SfcI CTRYAG 1 cut(s) 340
SfuI TTCGAA 1 cut(s) 996
SinI GGWCC 2 cut(s) 62, 831
SmiMI CAYNNNNRTG 2 cut(s) 268, 997
SmlI CTYRAG 2 cut(s) 66, 122
SmoI CTYRAG 2 cut(s) 66, 122
SpeI ACTAGT 1 cut(s) 1009
SseBI AGGCCT 1 cut(s) 1197
SsiI CCGC 1 cut(s) 1052
SspI AATATT 1 cut(s) 336
SspMI CTAG 4 cut(s) 900, 908, 1010, 1025
StuI AGGCCT 1 cut(s) 1197
StyD4I CCNGG 2 cut(s) 84, 833
TaaI ACNGT 3 cut(s) 503, 679, 1185
TaiI ACGT 4 cut(s) 156, 774, 915, 1143
TaqI TCGA 7 cut(s) 221, 354, 747, 934, 996, 1101, 1130
TfiI GAWTC 2 cut(s) 706, 866
Tru1I TTAA 2 cut(s) 123, 549
Tru9I TTAA 2 cut(s) 123, 549
TscAI CASTG 3 cut(s) 508, 723, 876
TseFI GTSAC 5 cut(s) 261, 503, 648, 711, 1088
Tsp45I GTSAC 5 cut(s) 261, 503, 648, 711, 1088
TspDTI ATGAA 6 cut(s) 64, 100, 702, 716, 766, 879
TspGWI ACGGA 1 cut(s) 464
TspRI CASTG 3 cut(s) 508, 723, 876
Van91I CCANNNNNTGG 2 cut(s) 807, 828
Vha464I CTTAAG 1 cut(s) 122
VpaK11BI GGWCC 2 cut(s) 62, 831
XapI RAATTY 5 cut(s) 380, 565, 689, 737, 856
XceI RCATGY 2 cut(s) 624, 1075
XcmI CCANNNNNNNNNTGG 1 cut(s) 581
XmiI GTMKAC 1 cut(s) 747
XmnI GAANNNNTTC 3 cut(s) 566, 569, 666
XspI CTAG 4 cut(s) 900, 908, 1010, 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.