AT1G70660

protein K63-linked ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014033
Physical Location & Seq
Forward (+)
26640565 .. 26642149
1585 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G70660.1

Sequence Viewer

Length: 480 bp
ATGGGTTCGGAAGAAGAGAAGGTCGTTGTTCCCAGAAACTTTAGATTATTGGAAGAGCTAGAAAGAGGTGAGAAAGGAATTGGAGATGGGACAGTGAGTTATGGAATGGATGATGCTGATGATATTTTGATGCAATCTTGGACTGGTACCATTCTTGGTCCTCATAATACTGCGTATGAAGGGAAAATCTTTCAGCTGAAGCTATTCTGTGGAAAGGATTATCCGGAAAGTCCACCTACTGTGAGGTTTCAGAGTCGTATAAACATGGCTTGTGTCAATCCTGAGAATGGAGTGGTTGATCCGAGTCACTTCCCTATGCTTTCCAACTGGAGAAGAGAATTCACAATGGAGGATCTACTGATTCAGCTTAAAAAAGAAATGATGTCATCGCAGAACCGCAAGTTAGCTCAACCCTTGGAAGGTAATGAGGAAGGCAGGACAGACCCAAAGGGACTAGTGGTGAAATGTTGCGTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000165 GO:0000187 GO:0000209 GO:0000726 GO:0000729 GO:0001932 GO:0001934 GO:0002218 GO:0002221 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002753 GO:0002757 GO:0002758 GO:0002764 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006281 GO:0006282 GO:0006301 GO:0006302 GO:0006303 GO:0006355 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006974 GO:0007154 GO:0007165 GO:0007166 GO:0007254 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0008283 GO:0009605 GO:0009607 GO:0009617 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010720 GO:0010941 GO:0010975 GO:0010976 GO:0010992 GO:0010994 GO:0016043 GO:0016310 GO:0016567 GO:0016740 GO:0019219 GO:0019220 GO:0019221 GO:0019222 GO:0019538 GO:0019725 GO:0019787 GO:0019899 GO:0022008 GO:0022607 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0030162 GO:0030522 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031344 GO:0031346 GO:0031347 GO:0031349 GO:0031371 GO:0031372 GO:0031399 GO:0031401 GO:0031624 GO:0031625 GO:0031974 GO:0031981 GO:0032147 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032501 GO:0032502 GO:0032879 GO:0032880 GO:0032991 GO:0033554 GO:0033674 GO:0034097 GO:0034622 GO:0034641 GO:0035370 GO:0035556 GO:0035872 GO:0036211 GO:0040012 GO:0042176 GO:0042221 GO:0042275 GO:0042325 GO:0042327 GO:0042592 GO:0042742 GO:0042981 GO:0042995 GO:0043005 GO:0043066 GO:0043067 GO:0043069 GO:0043085 GO:0043122 GO:0043123 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043523 GO:0043524 GO:0043549 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044390 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0045732 GO:0045739 GO:0045859 GO:0045860 GO:0045862 GO:0045893 GO:0045935 GO:0045937 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050776 GO:0050778 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050795 GO:0050803 GO:0050807 GO:0050896 GO:0051052 GO:0051054 GO:0051090 GO:0051091 GO:0051092 GO:0051094 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051258 GO:0051338 GO:0051347 GO:0051403 GO:0051704 GO:0051707 GO:0051716 GO:0051960 GO:0051962 GO:0051963 GO:0051965 GO:0060255 GO:0060284 GO:0060341 GO:0060548 GO:0061057 GO:0061136 GO:0061630 GO:0061631 GO:0061650 GO:0061659 GO:0065003 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070423 GO:0070498 GO:0070534 GO:0070555 GO:0070647 GO:0070887 GO:0071310 GO:0071345 GO:0071347 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090325 GO:0097458 GO:0098542 GO:0120025 GO:0120035 GO:0140096 GO:1901214 GO:1901215 GO:1901360 GO:1901564 GO:1901800 GO:1902494 GO:1902531 GO:1902533 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1903827 GO:1990234 GO:2000008 GO:2000026 GO:2000058 GO:2000060 GO:2000112 GO:2001020 GO:2001022 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01524 ko04624 map01524 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

159

Amino Acids

18.0

Weight (kDa)

5.03

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 41 - 134 1.2e-15 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23260 AT1G70660
fragaria_vesca FvH4_4g33750 FvH4_4g33750
malus_domestica MD16G1020200.v1.1
prunus_persica Prupe.1G332100_v2.0.a1 Prupe.1G332100_v2.0.a1 Prupe.1G332100_v2.0.a1 Prupe.1G332100_v2.0.a1
pyrus_communis pycom16g01810
rosa_chinensis RchiOBHm_Chr4g0443281
rosa_laevigata RLG00000005941
rosa_multiflora Rmu_sc0026145.1_g000001
rosa_roxburghii Rroxscaffold_5G00383870
rosa_rugosa Rorug04G0345300
rosa_samantha Rh4DG407100
rosa_wichuraiana Rw4G034520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 146
AccB1I GGYRCC 1 cut(s) 146
AccIII TCCGGA 1 cut(s) 223
AciI CCGC 1 cut(s) 397
AclWI GGATC 2 cut(s) 293, 360
AcsI RAATTY 1 cut(s) 338
AcuI CTGAAG 1 cut(s) 218
AfaI GTAC 1 cut(s) 148
AfiI CCNNNNNNNGG 2 cut(s) 287, 419
AhlI ACTAGT 1 cut(s) 454
AluBI AGCT 5 cut(s) 58, 196, 202, 367, 407
AluI AGCT 5 cut(s) 58, 196, 202, 367, 407
AlwI GGATC 2 cut(s) 293, 360
Aor13HI TCCGGA 1 cut(s) 223
ApoI RAATTY 1 cut(s) 338
Asp700I GAANNNNTTC 1 cut(s) 203
Asp718I GGTACC 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 158
AsuHPI GGTGA 2 cut(s) 80, 472
AvaII GGWCC 1 cut(s) 158
BanI GGYRCC 1 cut(s) 146
BccI CCATC 1 cut(s) 80
BcuI ACTAGT 1 cut(s) 454
BfaI CTAG 2 cut(s) 59, 455
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 1 cut(s) 148
BmsI GCATC 2 cut(s) 103, 120
BpmI CTGGAG 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 414
BsaWI WCCGGW 1 cut(s) 223
Bsc4I CCNNNNNNNGG 2 cut(s) 287, 419
Bse1I ACTGG 2 cut(s) 148, 332
BseAI TCCGGA 1 cut(s) 223
BseDI CCNNGG 1 cut(s) 414
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 2 cut(s) 287, 419
BseMII CTCAG 1 cut(s) 273
BseNI ACTGG 2 cut(s) 148, 332
BshNI GGYRCC 1 cut(s) 146
BsiSI CCGG 1 cut(s) 224
BslFI GGGAC 2 cut(s) 103, 465
BslI CCNNNNNNNGG 2 cut(s) 287, 419
BsmFI GGGAC 2 cut(s) 103, 465
Bsp13I TCCGGA 1 cut(s) 223
Bsp143I GATC 2 cut(s) 298, 352
BspACI CCGC 1 cut(s) 397
BspCNI CTCAG 1 cut(s) 274
BspEI TCCGGA 1 cut(s) 223
BspLI GGNNCC 1 cut(s) 148
BspPI GGATC 2 cut(s) 293, 360
BspQI GCTCTTC 1 cut(s) 48
BspT107I GGYRCC 1 cut(s) 146
BsrI ACTGG 2 cut(s) 148, 332
BssECI CCNNGG 1 cut(s) 414
BssMI GATC 2 cut(s) 298, 352
BssT1I CCWWGG 1 cut(s) 414
Bst4CI ACNGT 2 cut(s) 94, 241
Bst6I CTCTTC 3 cut(s) 9, 48, 328
BstDEI CTNAG 1 cut(s) 282
BstF5I GGATG 1 cut(s) 115
BstKTI GATC 2 cut(s) 301, 355
BstMBI GATC 2 cut(s) 298, 352
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BtgZI GCGATG 1 cut(s) 372
BtsCI GGATG 1 cut(s) 115
BtsIMutI CAGTG 1 cut(s) 99
Cfr13I GGNCC 1 cut(s) 158
Csp6I GTAC 1 cut(s) 147
CviAII CATG 1 cut(s) 265
CviJI RGCY 6 cut(s) 58, 196, 202, 269, 367, 407
CviKI_1 RGCY 6 cut(s) 58, 196, 202, 269, 367, 407
CviQI GTAC 1 cut(s) 147
DdeI CTNAG 1 cut(s) 282
DpnI GATC 2 cut(s) 300, 354
DpnII GATC 2 cut(s) 298, 352
Eam1104I CTCTTC 3 cut(s) 9, 48, 328
EarI CTCTTC 3 cut(s) 9, 48, 328
Eco130I CCWWGG 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 218
EcoRI GAATTC 1 cut(s) 338
EcoT14I CCWWGG 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 414
FaeI CATG 1 cut(s) 268
FaiI YATR 6 cut(s) 102, 165, 177, 260, 266, 317
FaqI GGGAC 2 cut(s) 103, 465
FatI CATG 1 cut(s) 264
FokI GGATG 1 cut(s) 122
FspBI CTAG 2 cut(s) 59, 455
GsuI CTGGAG 1 cut(s) 349
HapII CCGG 1 cut(s) 224
Hin1II CATG 1 cut(s) 268
HinfI GANTC 3 cut(s) 253, 304, 361
HpaII CCGG 1 cut(s) 224
HphI GGTGA 2 cut(s) 80, 472
Hpy166II GTNNAC 1 cut(s) 233
Hpy188I TCNGA 3 cut(s) 10, 252, 303
Hpy188III TCNNGA 2 cut(s) 224, 281
Hpy8I GTNNAC 1 cut(s) 233
HpyAV CCTTC 4 cut(s) 13, 173, 413, 425
HpyCH4III ACNGT 2 cut(s) 94, 241
HpyCH4V TGCA 1 cut(s) 133
HpyF3I CTNAG 1 cut(s) 282
Hsp92II CATG 1 cut(s) 268
Kpn2I TCCGGA 1 cut(s) 223
KpnI GGTACC 1 cut(s) 150
Kzo9I GATC 2 cut(s) 298, 352
LguI GCTCTTC 1 cut(s) 48
LpnPI CCDG 6 cut(s) 46, 129, 237, 294, 313, 421
LweI GCATC 2 cut(s) 103, 120
MaeI CTAG 2 cut(s) 59, 455
MaeIII GTNAC 1 cut(s) 305
MalI GATC 2 cut(s) 300, 354
MboI GATC 2 cut(s) 298, 352
MboII GAAGA 4 cut(s) 23, 26, 65, 345
MflI RGATCY 1 cut(s) 352
MluCI AATT 2 cut(s) 78, 338
MlyI GAGTC 2 cut(s) 262, 313
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 5 cut(s) 59, 171, 237, 343, 421
MroI TCCGGA 1 cut(s) 223
MroXI GAANNNNTTC 1 cut(s) 203
MseI TTAA 1 cut(s) 369
MspA1I CMGCKG 1 cut(s) 196
MspI CCGG 1 cut(s) 224
NdeII GATC 2 cut(s) 298, 352
NlaIII CATG 1 cut(s) 268
NlaIV GGNNCC 1 cut(s) 148
NmuCI GTSAC 1 cut(s) 305
PciSI GCTCTTC 1 cut(s) 48
PdmI GAANNNNTTC 1 cut(s) 203
PfeI GAWTC 1 cut(s) 361
PleI GAGTC 2 cut(s) 261, 312
PpsI GAGTC 2 cut(s) 261, 312
PspN4I GGNNCC 1 cut(s) 148
PspPI GGNCC 1 cut(s) 158
PsuI RGATCY 1 cut(s) 352
PvuII CAGCTG 1 cut(s) 196
RsaI GTAC 1 cut(s) 148
RsaNI GTAC 1 cut(s) 147
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 1 cut(s) 369
Sau3AI GATC 2 cut(s) 298, 352
Sau96I GGNCC 1 cut(s) 158
SchI GAGTC 2 cut(s) 262, 313
SfaNI GCATC 2 cut(s) 103, 120
SinI GGWCC 1 cut(s) 158
SpeI ACTAGT 1 cut(s) 454
Sse9I AATT 2 cut(s) 78, 338
SsiI CCGC 1 cut(s) 397
SspMI CTAG 2 cut(s) 59, 455
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 2 cut(s) 94, 241
TasI AATT 2 cut(s) 78, 338
TfiI GAWTC 1 cut(s) 361
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 1 cut(s) 99
TseFI GTSAC 1 cut(s) 305
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 1 cut(s) 192
TspRI CASTG 1 cut(s) 99
VpaK11BI GGWCC 1 cut(s) 158
XapI RAATTY 1 cut(s) 338
XmnI GAANNNNTTC 1 cut(s) 203
XspI CTAG 2 cut(s) 59, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.