MD16G1020200.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014033
Physical Location & Seq
Forward (+)
1454040 .. 1457237
3198 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1020200.v1.1.491

Sequence Viewer

Length: 480 bp
ATGGGCTCCGAAGGATCAAGCGTCGTCGTTCCCAGGAATTTCAGATTACTGGAAGAGCTTGAGAGGGGTGAAAAGGGAATTGGGGATGGCACTGTTAGCTATGGAATGGATGATGCTGATGATATTTATATGCAGTCATGGACAGGGACAATTATTGGACCTCCTAATACTGTGCACGAAGGGCGCATTTACCAGTTGAAACTGTTCTGTGGAAAGGACTATCCAGATAATCCACCAACTGTTAGGTTCCAAACACGAATAAACGTGACCTGTGTCAATCGTGATACTGGAGTGGTTGAACCCAGTCTTTTCCCCATGCTTGCCAATTGGCAAAGGGAATATACGATGGAGGACATATTGACACAATTGAAGAAAGAGATGATGTCACCACAAAACCGAAAGCTAGCTCAGCCACCTGAAGGCAATGAAGAGGGAAGGGTCGATCAAAAAGGGCTTGTGGTGAAATGTTGCATCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04624 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

160

Amino Acids

17.93

Weight (kDa)

4.97

Isoelectric Point (pI)

25.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 41 - 136 3.8e-16 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23260 AT1G70660
fragaria_vesca FvH4_4g33750 FvH4_4g33750
malus_domestica MD16G1020200.v1.1
prunus_persica Prupe.1G332100_v2.0.a1 Prupe.1G332100_v2.0.a1 Prupe.1G332100_v2.0.a1 Prupe.1G332100_v2.0.a1
pyrus_communis pycom16g01810
rosa_chinensis RchiOBHm_Chr4g0443281
rosa_laevigata RLG00000005941
rosa_multiflora Rmu_sc0026145.1_g000001
rosa_roxburghii Rroxscaffold_5G00383870
rosa_rugosa Rorug04G0345300
rosa_samantha Rh4DG407100
rosa_wichuraiana Rw4G034520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 22
AcsI RAATTY 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 438
AfiI CCNNNNNNNGG 1 cut(s) 419
AgsI TTSAA 3 cut(s) 199, 299, 370
AjnI CCWGG 1 cut(s) 32
AjuI GAANNNNNNNTTGG 2 cut(s) 63, 95
AluBI AGCT 4 cut(s) 58, 99, 403, 407
AluI AGCT 4 cut(s) 58, 99, 403, 407
Alw21I GWGCWC 1 cut(s) 177
Alw44I GTGCAC 1 cut(s) 173
AlwI GGATC 1 cut(s) 22
ApaLI GTGCAC 1 cut(s) 173
ApoI RAATTY 1 cut(s) 37
Asp700I GAANNNNTTC 1 cut(s) 203
AspLEI GCGC 1 cut(s) 186
AspS9I GGNCC 1 cut(s) 158
AsuHPI GGTGA 3 cut(s) 80, 378, 472
AsuNHI GCTAGC 1 cut(s) 403
AvaII GGWCC 1 cut(s) 158
BaeGI GKGCMC 1 cut(s) 177
BaeI ACNNNNGTAYC 2 cut(s) 276, 309
BanII GRGCYC 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 177
BccI CCATC 2 cut(s) 80, 340
BciT130I CCWGG 1 cut(s) 34
BfaI CTAG 1 cut(s) 404
BlpI GCTNAGC 1 cut(s) 408
Bme1390I CCNGG 1 cut(s) 34
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 2 cut(s) 7, 248
BmrFI CCNGG 1 cut(s) 34
BmrI ACTGGG 1 cut(s) 297
BmsI GCATC 1 cut(s) 103
BmtI GCTAGC 1 cut(s) 407
BmuI ACTGGG 1 cut(s) 297
BoxI GACNNNNGTC 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 309
Bpu1102I GCTNAGC 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 32
Bsc4I CCNNNNNNNGG 1 cut(s) 419
Bse1I ACTGG 4 cut(s) 54, 193, 292, 303
Bse3DI GCAATG 1 cut(s) 430
BseBI CCWGG 1 cut(s) 34
BseDI CCNNGG 1 cut(s) 32
BseGI GGATG 2 cut(s) 91, 115
BseLI CCNNNNNNNGG 1 cut(s) 419
BseMI GCAATG 1 cut(s) 430
BseMII CTCAG 1 cut(s) 422
BseNI ACTGG 4 cut(s) 54, 193, 292, 303
BseSI GKGCMC 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 177
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 1 cut(s) 419
BsmFI GGGAC 1 cut(s) 160
Bsp1286I GDGCHC 2 cut(s) 8, 177
Bsp143I GATC 2 cut(s) 14, 442
Bsp1720I GCTNAGC 1 cut(s) 408
BspCNI CTCAG 1 cut(s) 421
BspLI GGNNCC 2 cut(s) 7, 248
BspOI GCTAGC 1 cut(s) 407
BspPI GGATC 1 cut(s) 22
BspQI GCTCTTC 1 cut(s) 48
BsrDI GCAATG 1 cut(s) 430
BsrI ACTGG 4 cut(s) 54, 193, 292, 303
BssECI CCNNGG 1 cut(s) 32
BssMI GATC 2 cut(s) 14, 442
Bst2UI CCWGG 1 cut(s) 34
Bst4CI ACNGT 4 cut(s) 94, 172, 204, 241
Bst6I CTCTTC 2 cut(s) 48, 423
BstC8I GCNNGC 2 cut(s) 321, 405
BstDEI CTNAG 1 cut(s) 408
BstF5I GGATG 2 cut(s) 91, 115
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 2 cut(s) 17, 445
BstMBI GATC 2 cut(s) 14, 442
BstMWI GCNNNNNNNGC 3 cut(s) 96, 181, 409
BstNI CCWGG 1 cut(s) 34
BstPAI GACNNNNGTC 1 cut(s) 272
BstSCI CCNGG 1 cut(s) 32
BstSLI GKGCMC 1 cut(s) 177
BtsCI GGATG 2 cut(s) 91, 115
BtsIMutI CAGTG 1 cut(s) 90
Cac8I GCNNGC 2 cut(s) 321, 405
CfoI GCGC 1 cut(s) 186
Cfr13I GGNCC 1 cut(s) 158
CseI GACGC 1 cut(s) 10
CviAII CATG 2 cut(s) 138, 316
CviJI RGCY 7 cut(s) 6, 58, 99, 403, 407, 412, 454
CviKI_1 RGCY 7 cut(s) 6, 58, 99, 403, 407, 412, 454
DdeI CTNAG 1 cut(s) 408
DpnI GATC 2 cut(s) 16, 444
DpnII GATC 2 cut(s) 14, 442
Eam1104I CTCTTC 2 cut(s) 48, 423
EarI CTCTTC 2 cut(s) 48, 423
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 32
EcoT38I GRGCYC 1 cut(s) 8
FaeI CATG 2 cut(s) 141, 319
FaiI YATR 7 cut(s) 102, 129, 131, 139, 317, 342, 356
FaqI GGGAC 1 cut(s) 160
FatI CATG 2 cut(s) 137, 315
FokI GGATG 2 cut(s) 98, 122
FriOI GRGCYC 1 cut(s) 8
FspBI CTAG 1 cut(s) 404
GlaI GCGC 1 cut(s) 185
GsuI CTGGAG 1 cut(s) 309
HgaI GACGC 1 cut(s) 10
HhaI GCGC 1 cut(s) 186
Hin1II CATG 2 cut(s) 141, 319
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HphI GGTGA 3 cut(s) 80, 378, 472
Hpy166II GTNNAC 1 cut(s) 175
Hpy188I TCNGA 2 cut(s) 10, 44
Hpy188III TCNNGA 2 cut(s) 224, 281
Hpy8I GTNNAC 1 cut(s) 175
Hpy99I CGWCG 2 cut(s) 26, 29
HpyAV CCTTC 4 cut(s) 5, 173, 413, 429
HpyCH4III ACNGT 4 cut(s) 94, 172, 204, 241
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 3 cut(s) 133, 175, 471
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 181, 409
HpyF3I CTNAG 1 cut(s) 408
HpySE526I ACGT 1 cut(s) 264
Hsp92II CATG 2 cut(s) 141, 319
HspAI GCGC 1 cut(s) 184
Kzo9I GATC 2 cut(s) 14, 442
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 1 cut(s) 11
LweI GCATC 1 cut(s) 103
MaeI CTAG 1 cut(s) 404
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 2 cut(s) 265, 384
MalI GATC 2 cut(s) 16, 444
MboI GATC 2 cut(s) 14, 442
MboII GAAGA 3 cut(s) 65, 382, 440
MfeI CAATTG 2 cut(s) 325, 365
MhlI GDGCHC 2 cut(s) 8, 177
MluCI AATT 5 cut(s) 37, 78, 150, 325, 365
MnlI CCTC 4 cut(s) 57, 171, 343, 424
MroXI GAANNNNTTC 1 cut(s) 203
MspR9I CCNGG 1 cut(s) 34
MunI CAATTG 2 cut(s) 325, 365
MvaI CCWGG 1 cut(s) 34
MwoI GCNNNNNNNGC 3 cut(s) 96, 181, 409
NdeII GATC 2 cut(s) 14, 442
NheI GCTAGC 1 cut(s) 403
NlaIII CATG 2 cut(s) 141, 319
NlaIV GGNNCC 2 cut(s) 7, 248
NmuCI GTSAC 2 cut(s) 265, 384
PciSI GCTCTTC 1 cut(s) 48
PdmI GAANNNNTTC 1 cut(s) 203
PshAI GACNNNNGTC 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 32
PspGI CCWGG 1 cut(s) 32
PspN4I GGNNCC 2 cut(s) 7, 248
PspPI GGNCC 1 cut(s) 158
SapI GCTCTTC 1 cut(s) 48
Sau3AI GATC 2 cut(s) 14, 442
Sau96I GGNCC 1 cut(s) 158
ScrFI CCNGG 1 cut(s) 34
SduI GDGCHC 2 cut(s) 8, 177
SetI ASST 9 cut(s) 60, 101, 163, 248, 267, 272, 405, 409, 418
SfaNI GCATC 1 cut(s) 103
SinI GGWCC 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 5 cut(s) 37, 78, 150, 325, 365
SspMI CTAG 1 cut(s) 404
StyD4I CCNGG 1 cut(s) 32
TaaI ACNGT 4 cut(s) 94, 172, 204, 241
TaiI ACGT 1 cut(s) 267
TaqI TCGA 1 cut(s) 441
TasI AATT 5 cut(s) 37, 78, 150, 325, 365
TscAI CASTG 1 cut(s) 97
TseFI GTSAC 2 cut(s) 265, 384
Tsp45I GTSAC 2 cut(s) 265, 384
TspDTI ATGAA 1 cut(s) 441
TspRI CASTG 1 cut(s) 97
VneI GTGCAC 1 cut(s) 173
VpaK11BI GGWCC 1 cut(s) 158
XapI RAATTY 1 cut(s) 37
XmnI GAANNNNTTC 1 cut(s) 203
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.