AT1G72990

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010424
Physical Location & Seq
Reverse (-)
27457266 .. 27462487
5222 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G72990.4

Sequence Viewer

Length: 2094 bp
ATGGCGATGACAAGCTGGCCATCAACGGGAAGACAAAGAAGACATCAATTGGCTTCCATGTTGCTCCTGGTTCTTGTAGTTGTCGGAATCTATGTTCCTGTCTTCGCTCTTCTCCCTTCACTGTCATATACTCCTCAGTCTCTACCTTCTGCTATACCTCAGGACGAGAAGATGATATCTCGCAAGTTTTATATCAAGGATGACAATTTCTGGAAAGATGGGAATCGTTTTCAGATCATTGGTGGTGATTTGCATTACTTTCGTGTTCTTCCAGAGTACTGGGAGGATCGGCTTTTAAGAGCCAATGCTTTAGGCTTGAATACTATTCAAGTATACGTTCCTTGGAATCTGCATGAACCAAAGCCTGGGAAGATGGTTTTTGAGGGTATTGGTGATCTTGTCTCCTTTCTCAAGCTGTGTGAGAAACTAGATTTTCTGGTTATGCTACGTGCTGGACCTTATATCTGTGGAGAATGGGATTTGGGAGGCTTTCCTGCTTGGTTACTTGCTGTGAAACCACGTCTCCAACTAAGGACATCAGATCCTGTATATCTTAAGTTGGTTGAAAGATGGTGGGATGTTCTGTTACCGAAGGTATTTCCTCTGCTTTACAGCAACGGGGGTCCTGTTATAATGGTTCAGATTGAAAATGAATATGGTTCATATGGAAATGACAAAGCTTATCTTCGTAAGCTAGTTAGTATGGCTAGGGGACACCTGGGGGATGACATTATTGTGTACACAACAGATGGAGGGACAAAAGAAACTCTTGATAAAGGAACTGTCCCTGTAGCTGATGTCTACTCAGCTGTTGATTTCTCTACCGGTGATGATCCTTGGCCAATATTTAAATTGCAAAAGAAGTTTAATGCTCCAGGAAGATCACCTCCACTTTCCTCGGAGTTCTACACTGGTTGGCTCACGCATTGGGGTGAGAAAATTACAAAAACAGATGCCGAATTTACGGCAGCTTCTCTTGAAAAAATTCTGTCTCGAAATGGTTCTGCTGTGCTCTATATGGTGCATGGAGGAACAAACTTTGGTTTCTACAATGGCGCAAATACTGGCTCTGAAGAATCTGACTATAAACCAGATCTGACATCTTATGACTATGATGCTCCCATTAAGGAGTCTGGTGACATAGATAATCCAAAATTCCAAGCTCTTCAGAGAGTGATTAAGAAGTACAATGCTTCACCACACCCCATTAGCCCATCCAATAAGCAACGGAAAGCTTATGGCTCTATCAAGATGCAGATGACAACATCACTGTTTGATTTAGTTCGTATGACAGATCCTGCAGATGTGATCACCTCCGCCAACCCAATTTCAATGGAATCTGTTGGACAGATGTTCGGATTTCTTTTATATGAATCTTCATACATCGCAAAAAAGAGTGGGAATACACTAAGAATACCCAAGGTTCATGACAGAGCTCAAGTGTTTGTTTCATGTCTTTCCCAAGATGTTGATGTGGGAGTACTGAGATACATTGGTACTACTGAGAGATGGAACAACCAACCTATTTCTCTTCCAACTATTGAGTGTACCACAAACACTAGCTTATTTATTCTGGTTGAAAACATGGGTCGTGTAAATTATGGGCCATATATCTTTGATGACAAGGGTATTTTGTCTTCGGTTTATCTAGATGGTCAAATTCTCCATGGATGGAAGATGATACCAATTCCTTTTCACAATCTAAATCAAGAGCCAAATCTCACTTTCGAGATGCAACATACCAAAAACAGAAGTAAAAAGTTCGAGCTTACTAATGATGTTGGTCGAAAGGAACCCGCTCTGTTTGCTGGTGAATTCTCTATCAACAGTGAAGAAGAAATCAAAGACACATATCTATCATTCAACGGTTGGGGTAAAGGAGTTGCTTTTGTCAATGAATTCAACATTGGAAGATATTGGCCGTCTGTTGGACCACAGTGCAACCTCTATGTTCCTGCGCCACTTCTTAAGCGTGGCAAAAACACTTTGGTGGTATTCGAGTTGGAATCTCCGCATCTCGAGCTTTCGCTGGAGGCAGTGGATCACCAAGACTTCACATGCGGTTCAAATGTTTCCAAAGTTAATCAGTTGTAG

Protein Analysis

697

Amino Acids

78.64

Weight (kDa)

6.27

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 70 - 395 1.7e-119 Glycosyl hydrolases family 35
BetaGal_ABD_1 PF21317 442 - 563 1.9e-24 Beta-galactosidase, first all-beta domain
BetaGal_gal-bd PF21467 599 - 661 2.6e-22 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72990 AT1G72990 AT1G72990 AT1G72990
fragaria_vesca FvH4_6g33540 FvH4_6g33540 FvH4_6g33540
malus_domestica MD09G1192500.v1.1
prunus_persica Prupe.3G048800_v2.0.a1 Prupe.3G048800_v2.0.a1
pyrus_communis pycom09g10910
rosa_chinensis RchiOBHm_Chr2g0143451
rosa_laevigata RLG00000020036
rosa_multiflora Rmu_sc0003507.1_g000021
rosa_roxburghii Rroxscaffold_2G00101830
rosa_rugosa Rorug02G0381500
rosa_samantha Rh2AG434500 Rh2BG442300 Rh2CG420400 Rh2DG452500
rosa_wichuraiana Rw2G035420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 632
AccB7I CCANNNNNTGG 1 cut(s) 365
AccBSI CCGCTC 1 cut(s) 1799
AccI GTMKAC 2 cut(s) 333, 801
AciI CCGC 4 cut(s) 1317, 1797, 2012, 2061
AclWI GGATC 5 cut(s) 294, 536, 827, 1289, 2049
AcoI YGGCCR 3 cut(s) 17, 839, 1919
AcsI RAATTY 6 cut(s) 959, 984, 1154, 1659, 1814, 1898
AcuI CTGAAG 2 cut(s) 1092, 1151
AfaI GTAC 6 cut(s) 278, 740, 1187, 1482, 1498, 1549
AfiI CCNNNNNNNGG 3 cut(s) 26, 365, 1928
AflII CTTAAG 2 cut(s) 554, 1967
AgeI ACCGGT 1 cut(s) 824
AjiI CACGTC 1 cut(s) 521
AjnI CCWGG 4 cut(s) 66, 364, 717, 874
AjuI GAANNNNNNNTTGG 2 cut(s) 1890, 1922
AleI CACNNNNGTG 1 cut(s) 1988
AloI GAACNNNNNNTCC 2 cut(s) 78, 110
Alw21I GWGCWC 2 cut(s) 1014, 1438
Alw26I GTCTC 4 cut(s) 144, 406, 527, 996
AlwI GGATC 5 cut(s) 294, 536, 827, 1289, 2049
AlwNI CAGNNNCTG 2 cut(s) 545, 1298
Ama87I CYCGRG 1 cut(s) 2018
AoxI GGCC 4 cut(s) 17, 839, 1604, 1919
ApeKI GCWGC 1 cut(s) 968
ApoI RAATTY 6 cut(s) 959, 984, 1154, 1659, 1814, 1898
AsiGI ACCGGT 1 cut(s) 824
Asp700I GAANNNNTTC 2 cut(s) 984, 1000
AspLEI GCGC 2 cut(s) 1058, 1960
AspS9I GGNCC 4 cut(s) 455, 623, 1604, 1931
AvaI CYCGRG 1 cut(s) 2018
AvaII GGWCC 3 cut(s) 455, 623, 1931
AxyI CCTNAGG 1 cut(s) 159
BaeI ACNNNNGTAYC 2 cut(s) 1480, 1513
BalI TGGCCA 2 cut(s) 19, 841
BanII GRGCYC 1 cut(s) 1438
BarI GAAGNNNNNNTAC 2 cut(s) 1621, 1653
BbsI GAAGAC 4 cut(s) 37, 46, 94, 1629
Bbv12I GWGCWC 2 cut(s) 1014, 1438
BbvI GCAGC 1 cut(s) 980
BccI CCATC 9 cut(s) 28, 212, 367, 564, 743, 1222, 1503, 1646, 1665
BceAI ACGGC 2 cut(s) 981, 1906
BcgI CGANNNNNNTGC 2 cut(s) 242, 276
BciT130I CCWGG 4 cut(s) 68, 366, 719, 876
BclI TGATCA 1 cut(s) 1308
BcoDI GTCTC 4 cut(s) 144, 406, 527, 996
BfaI CTAG 5 cut(s) 428, 695, 708, 1560, 1649
BfmI CTRYAG 2 cut(s) 789, 1299
BfrI CTTAAG 2 cut(s) 554, 1967
BglII AGATCT 1 cut(s) 1093
BisI GCNGC 1 cut(s) 969
BlsI GCNGC 1 cut(s) 970
BmcAI AGTACT 2 cut(s) 278, 1482
Bme1390I CCNGG 4 cut(s) 68, 366, 719, 876
Bme18I GGWCC 3 cut(s) 455, 623, 1931
BmeT110I CYCGRG 1 cut(s) 2018
BmgBI CACGTC 1 cut(s) 521
BmgT120I GGNCC 4 cut(s) 455, 623, 1604, 1931
BmiI GGNNCC 2 cut(s) 624, 1794
BmrFI CCNGG 4 cut(s) 68, 366, 719, 876
BmrI ACTGGG 1 cut(s) 289
BmsI GCATC 5 cut(s) 943, 1105, 1242, 1722, 2023
BmuI ACTGGG 1 cut(s) 289
BpiI GAAGAC 4 cut(s) 37, 46, 94, 1629
BpmI CTGGAG 2 cut(s) 858, 2051
BpuEI CTTGAG 2 cut(s) 395, 1422
BsaAI YACGTR 1 cut(s) 449
BsaBI GATNNNNATC 1 cut(s) 222
BsaJI CCNNGG 7 cut(s) 341, 365, 718, 836, 897, 1419, 1666
BsaWI WCCGGW 1 cut(s) 824
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 365, 1928
Bse118I RCCGGY 1 cut(s) 824
Bse1I ACTGG 3 cut(s) 284, 916, 1069
Bse21I CCTNAGG 1 cut(s) 159
Bse8I GATNNNNATC 1 cut(s) 222
BseBI CCWGG 4 cut(s) 68, 366, 719, 876
BseDI CCNNGG 7 cut(s) 341, 365, 718, 836, 897, 1419, 1666
BseGI GGATG 5 cut(s) 205, 583, 730, 1214, 1676
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 3 cut(s) 26, 365, 1928
BseMII CTCAG 5 cut(s) 149, 173, 819, 1475, 1494
BseNI ACTGG 3 cut(s) 284, 916, 1069
BseRI GAGGAG 1 cut(s) 123
BseXI GCAGC 1 cut(s) 980
BshFI GGCC 4 cut(s) 19, 841, 1606, 1921
BshTI ACCGGT 1 cut(s) 824
BsiHKAI GWGCWC 2 cut(s) 1014, 1438
BsiHKCI CYCGRG 1 cut(s) 2018
BsiSI CCGG 1 cut(s) 825
BslFI GGGAC 3 cut(s) 726, 769, 770
BslI CCNNNNNNNGG 3 cut(s) 26, 365, 1928
BsmAI GTCTC 4 cut(s) 144, 406, 527, 996
BsmBI CGTCTC 1 cut(s) 527
BsmFI GGGAC 3 cut(s) 726, 769, 770
BsnI GGCC 4 cut(s) 19, 841, 1606, 1921
BsoBI CYCGRG 1 cut(s) 2018
Bsp1286I GDGCHC 2 cut(s) 1014, 1438
Bsp1407I TGTACA 1 cut(s) 738
Bsp19I CCATGG 1 cut(s) 1666
BspACI CCGC 4 cut(s) 1317, 1797, 2012, 2061
BspANI GGCC 4 cut(s) 19, 841, 1606, 1921
BspCNI CTCAG 5 cut(s) 148, 172, 818, 1476, 1495
BspHI TCATGA 1 cut(s) 1426
BspLI GGNNCC 2 cut(s) 624, 1794
BspMAI CTGCAG 1 cut(s) 1303
BspPI GGATC 5 cut(s) 294, 536, 827, 1289, 2049
BspQI GCTCTTC 2 cut(s) 114, 1170
BspTI CTTAAG 2 cut(s) 554, 1967
BsrBI CCGCTC 1 cut(s) 1799
BsrFI RCCGGY 1 cut(s) 824
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 3 cut(s) 284, 916, 1069
BssAI RCCGGY 1 cut(s) 824
BssECI CCNNGG 7 cut(s) 341, 365, 718, 836, 897, 1419, 1666
BssNAI GTATAC 1 cut(s) 334
BssT1I CCWWGG 4 cut(s) 341, 836, 1419, 1666
Bst1107I GTATAC 1 cut(s) 334
Bst2UI CCWGG 4 cut(s) 68, 366, 719, 876
Bst4CI ACNGT 6 cut(s) 123, 784, 1272, 1829, 1868, 1938
Bst6I CTCTTC 3 cut(s) 114, 1170, 1536
BstAFI CTTAAG 2 cut(s) 554, 1967
BstAUI TGTACA 1 cut(s) 738
BstBAI YACGTR 1 cut(s) 449
BstC8I GCNNGC 1 cut(s) 17
BstDEI CTNAG 7 cut(s) 135, 159, 530, 805, 1409, 1484, 1503
BstDSI CCRYGG 1 cut(s) 1666
BstF5I GGATG 5 cut(s) 205, 583, 730, 1214, 1676
BstHHI GCGC 2 cut(s) 1058, 1960
BstMAI GTCTC 4 cut(s) 144, 406, 527, 996
BstMWI GCNNNNNNNGC 2 cut(s) 1805, 2020
BstNI CCWGG 4 cut(s) 68, 366, 719, 876
BstNSI RCATGY 1 cut(s) 2061
BstSCI CCNGG 4 cut(s) 66, 364, 717, 874
BstSFI CTRYAG 2 cut(s) 789, 1299
BstV1I GCAGC 1 cut(s) 980
BstV2I GAAGAC 4 cut(s) 37, 46, 94, 1629
BstX2I RGATCY 3 cut(s) 541, 1093, 1294
BstXI CCANNNNNNTGG 1 cut(s) 279
BstYI RGATCY 3 cut(s) 541, 1093, 1294
BstZ17I GTATAC 1 cut(s) 334
Bsu36I CCTNAGG 1 cut(s) 159
BsuRI GGCC 4 cut(s) 19, 841, 1606, 1921
BtgI CCRYGG 1 cut(s) 1666
BtgZI GCGATG 2 cut(s) 20, 1369
BtrI CACGTC 1 cut(s) 521
BtsCI GGATG 5 cut(s) 205, 583, 730, 1214, 1676
BtsI GCAGTG 1 cut(s) 2043
BtsIMutI CAGTG 6 cut(s) 119, 909, 1268, 1834, 1943, 2043
Cac8I GCNNGC 1 cut(s) 17
CaiI CAGNNNCTG 2 cut(s) 545, 1298
CciI TCATGA 1 cut(s) 1426
CfoI GCGC 2 cut(s) 1058, 1960
Cfr10I RCCGGY 1 cut(s) 824
Cfr13I GGNCC 4 cut(s) 455, 623, 1604, 1931
Csp6I GTAC 6 cut(s) 277, 739, 1186, 1481, 1497, 1548
CspAI ACCGGT 1 cut(s) 824
CviAII CATG 8 cut(s) 58, 353, 1025, 1427, 1452, 1585, 1667, 2058
CviQI GTAC 6 cut(s) 277, 739, 1186, 1481, 1497, 1548
DdeI CTNAG 7 cut(s) 135, 159, 530, 805, 1409, 1484, 1503
DraI TTTAAA 1 cut(s) 850
EaeI YGGCCR 3 cut(s) 17, 839, 1919
Eam1104I CTCTTC 3 cut(s) 114, 1170, 1536
EarI CTCTTC 3 cut(s) 114, 1170, 1536
EciI GGCGGA 1 cut(s) 1306
Ecl136II GAGCTC 1 cut(s) 1436
Eco130I CCWWGG 4 cut(s) 341, 836, 1419, 1666
Eco24I GRGCYC 1 cut(s) 1438
Eco32I GATATC 1 cut(s) 177
Eco47I GGWCC 3 cut(s) 455, 623, 1931
Eco53kI GAGCTC 1 cut(s) 1436
Eco57I CTGAAG 2 cut(s) 1092, 1151
Eco81I CCTNAGG 1 cut(s) 159
Eco88I CYCGRG 1 cut(s) 2018
EcoICRI GAGCTC 1 cut(s) 1436
EcoO109I RGGNCCY 1 cut(s) 623
EcoRI GAATTC 2 cut(s) 1814, 1898
EcoRII CCWGG 4 cut(s) 66, 364, 717, 874
EcoRV GATATC 1 cut(s) 177
EcoT14I CCWWGG 4 cut(s) 341, 836, 1419, 1666
EcoT38I GRGCYC 1 cut(s) 1438
ErhI CCWWGG 4 cut(s) 341, 836, 1419, 1666
Esp3I CGTCTC 1 cut(s) 527
FaeI CATG 8 cut(s) 61, 356, 1028, 1430, 1455, 1588, 1670, 2061
FalI AAGNNNNNCTT 4 cut(s) 669, 701, 753, 785
FaqI GGGAC 3 cut(s) 726, 769, 770
FatI CATG 8 cut(s) 57, 352, 1024, 1426, 1451, 1584, 1666, 2057
FauI CCCGC 1 cut(s) 1804
FauNDI CATATG 1 cut(s) 664
FbaI TGATCA 1 cut(s) 1308
FblI GTMKAC 2 cut(s) 333, 801
Fnu4HI GCNGC 1 cut(s) 969
FokI GGATG 5 cut(s) 212, 590, 737, 1201, 1683
FriOI GRGCYC 1 cut(s) 1438
Fsp4HI GCNGC 1 cut(s) 969
FspBI CTAG 5 cut(s) 428, 695, 708, 1560, 1649
GlaI GCGC 2 cut(s) 1057, 1959
GluI GCNGC 1 cut(s) 969
GsuI CTGGAG 2 cut(s) 858, 2051
HaeIII GGCC 4 cut(s) 19, 841, 1606, 1921
HapII CCGG 1 cut(s) 825
HhaI GCGC 2 cut(s) 1058, 1960
Hin1II CATG 8 cut(s) 61, 356, 1028, 1430, 1455, 1588, 1670, 2061
Hin6I GCGC 2 cut(s) 1056, 1958
HinP1I GCGC 2 cut(s) 1056, 1958
HindIII AAGCTT 2 cut(s) 678, 1233
HinfI GANTC 8 cut(s) 87, 223, 346, 1076, 1130, 1337, 1373, 2006
HpaII CCGG 1 cut(s) 825
Hpy166II GTNNAC 5 cut(s) 334, 739, 741, 802, 1548
Hpy8I GTNNAC 5 cut(s) 334, 739, 741, 802, 1548
HpyAV CCTTC 3 cut(s) 126, 156, 586
HpyCH4III ACNGT 6 cut(s) 123, 784, 1272, 1829, 1868, 1938
HpyCH4IV ACGT 3 cut(s) 336, 448, 520
HpyCH4V TGCA 8 cut(s) 253, 352, 856, 1024, 1255, 1301, 1735, 1941
HpyF10VI GCNNNNNNNGC 2 cut(s) 1805, 2020
HpyF3I CTNAG 7 cut(s) 135, 159, 530, 805, 1409, 1484, 1503
HpySE526I ACGT 3 cut(s) 336, 448, 520
Hsp92II CATG 8 cut(s) 61, 356, 1028, 1430, 1455, 1588, 1670, 2061
HspAI GCGC 2 cut(s) 1056, 1958
Ksp22I TGATCA 1 cut(s) 1308
LguI GCTCTTC 2 cut(s) 114, 1170
LmnI GCTCC 3 cut(s) 69, 877, 1123
Lsp1109I GCAGC 1 cut(s) 980
LweI GCATC 5 cut(s) 943, 1105, 1242, 1722, 2023
MaeI CTAG 5 cut(s) 428, 695, 708, 1560, 1649
MaeII ACGT 3 cut(s) 336, 448, 520
MaeIII GTNAC 3 cut(s) 501, 585, 1136
MbiI CCGCTC 1 cut(s) 1799
MfeI CAATTG 1 cut(s) 47
MflI RGATCY 3 cut(s) 541, 1093, 1294
MhlI GDGCHC 2 cut(s) 1014, 1438
MlsI TGGCCA 2 cut(s) 19, 841
MluNI TGGCCA 2 cut(s) 19, 841
MlyI GAGTC 1 cut(s) 1139
MmeI TCCRAC 6 cut(s) 64, 550, 1324, 1559, 1909, 1983
Mox20I TGGCCA 2 cut(s) 19, 841
MroXI GAANNNNTTC 2 cut(s) 984, 1000
MscI TGGCCA 2 cut(s) 19, 841
MseI TTAA 8 cut(s) 296, 555, 849, 867, 1125, 1179, 1968, 2082
MslI CAYNNNNRTG 3 cut(s) 734, 930, 1988
Msp20I TGGCCA 2 cut(s) 19, 841
MspA1I CMGCKG 1 cut(s) 809
MspCI CTTAAG 2 cut(s) 554, 1967
MspI CCGG 1 cut(s) 825
MspR9I CCNGG 4 cut(s) 68, 366, 719, 876
MunI CAATTG 1 cut(s) 47
MvaI CCWGG 4 cut(s) 68, 366, 719, 876
MwoI GCNNNNNNNGC 2 cut(s) 1805, 2020
NcoI CCATGG 1 cut(s) 1666
NdeI CATATG 1 cut(s) 664
NlaIII CATG 8 cut(s) 61, 356, 1028, 1430, 1455, 1588, 1670, 2061
NlaIV GGNNCC 2 cut(s) 624, 1794
NmuCI GTSAC 1 cut(s) 1136
NspI RCATGY 1 cut(s) 2061
OliI CACNNNNGTG 1 cut(s) 1988
PaeR7I CTCGAG 1 cut(s) 2018
PagI TCATGA 1 cut(s) 1426
PciSI GCTCTTC 2 cut(s) 114, 1170
PdmI GAANNNNTTC 2 cut(s) 984, 1000
PfeI GAWTC 7 cut(s) 87, 223, 346, 1076, 1337, 1373, 2006
PflMI CCANNNNNTGG 1 cut(s) 365
PfoI TCCNGGA 1 cut(s) 874
PinAI ACCGGT 1 cut(s) 824
PkrI GCNGC 1 cut(s) 970
PleI GAGTC 1 cut(s) 1138
PpsI GAGTC 1 cut(s) 1138
Ppu21I YACGTR 1 cut(s) 449
PpuMI RGGWCCY 1 cut(s) 623
PsiI TTATAA 1 cut(s) 632
Psp124BI GAGCTC 1 cut(s) 1438
Psp5II RGGWCCY 1 cut(s) 623
Psp6I CCWGG 4 cut(s) 66, 364, 717, 874
PspGI CCWGG 4 cut(s) 66, 364, 717, 874
PspN4I GGNNCC 2 cut(s) 624, 1794
PspPI GGNCC 4 cut(s) 455, 623, 1604, 1931
PspPPI RGGWCCY 1 cut(s) 623
PstI CTGCAG 1 cut(s) 1303
PstNI CAGNNNCTG 2 cut(s) 545, 1298
PsuI RGATCY 3 cut(s) 541, 1093, 1294
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 6 cut(s) 278, 740, 1187, 1482, 1498, 1549
RsaNI GTAC 6 cut(s) 277, 739, 1186, 1481, 1497, 1548
RseI CAYNNNNRTG 3 cut(s) 734, 930, 1988
SacI GAGCTC 1 cut(s) 1438
SapI GCTCTTC 2 cut(s) 114, 1170
SaqAI TTAA 8 cut(s) 296, 555, 849, 867, 1125, 1179, 1968, 2082
SatI GCNGC 1 cut(s) 969
Sau96I GGNCC 4 cut(s) 455, 623, 1604, 1931
ScaI AGTACT 2 cut(s) 278, 1482
SchI GAGTC 1 cut(s) 1139
ScrFI CCNGG 4 cut(s) 68, 366, 719, 876
SduI GDGCHC 2 cut(s) 1014, 1438
SfaNI GCATC 5 cut(s) 943, 1105, 1242, 1722, 2023
SfcI CTRYAG 2 cut(s) 789, 1299
Sfr274I CTCGAG 1 cut(s) 2018
SinI GGWCC 3 cut(s) 455, 623, 1931
SlaI CTCGAG 1 cut(s) 2018
SmiI ATTTAAAT 1 cut(s) 850
SmiMI CAYNNNNRTG 3 cut(s) 734, 930, 1988
SmlI CTYRAG 5 cut(s) 410, 554, 1437, 1967, 2018
SmoI CTYRAG 5 cut(s) 410, 554, 1437, 1967, 2018
SsiI CCGC 4 cut(s) 1317, 1797, 2012, 2061
SspI AATATT 1 cut(s) 846
SspMI CTAG 5 cut(s) 428, 695, 708, 1560, 1649
SstI GAGCTC 1 cut(s) 1438
StyD4I CCNGG 4 cut(s) 66, 364, 717, 874
StyI CCWWGG 4 cut(s) 341, 836, 1419, 1666
SwaI ATTTAAAT 1 cut(s) 850
TaaI ACNGT 6 cut(s) 123, 784, 1272, 1829, 1868, 1938
TaiI ACGT 3 cut(s) 339, 451, 523
TaqI TCGA 6 cut(s) 994, 1728, 1764, 1786, 1998, 2019
TatI WGTACW 4 cut(s) 276, 738, 1185, 1480
TfiI GAWTC 7 cut(s) 87, 223, 346, 1076, 1337, 1373, 2006
Tru1I TTAA 8 cut(s) 296, 555, 849, 867, 1125, 1179, 1968, 2082
Tru9I TTAA 8 cut(s) 296, 555, 849, 867, 1125, 1179, 1968, 2082
TscAI CASTG 6 cut(s) 126, 916, 1275, 1834, 1943, 2043
TseFI GTSAC 1 cut(s) 1136
TseI GCWGC 1 cut(s) 968
Tsp45I GTSAC 1 cut(s) 1136
TspDTI ATGAA 8 cut(s) 369, 651, 666, 1368, 1386, 1415, 1440, 1911
TspGWI ACGGA 1 cut(s) 1243
TspRI CASTG 6 cut(s) 126, 916, 1275, 1834, 1943, 2043
Van91I CCANNNNNTGG 1 cut(s) 365
Vha464I CTTAAG 2 cut(s) 554, 1967
VpaK11BI GGWCC 3 cut(s) 455, 623, 1931
XapI RAATTY 6 cut(s) 959, 984, 1154, 1659, 1814, 1898
XbaI TCTAGA 1 cut(s) 1648
XceI RCATGY 1 cut(s) 2061
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XhoI CTCGAG 1 cut(s) 2018
XmiI GTMKAC 2 cut(s) 333, 801
XmnI GAANNNNTTC 2 cut(s) 984, 1000
XspI CTAG 5 cut(s) 428, 695, 708, 1560, 1649
ZrmI AGTACT 2 cut(s) 278, 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.