RLG00000020036

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010424
Physical Location & Seq
Reverse (-)
60351702 .. 60357790
6089 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020036

Sequence Viewer

Length: 2055 bp
ATGGCCAGGAAGAGAAGCACAAGACTCACCTTGTTCTTCTTCCTCCTCCTCTCTCTCATAGCCTTCGGGGTCTTTGTCCCTGTCTTTGCTCTTATACCTTCTCTTTCCCAATCTCAAAATGACCAACAGCACCATCCAACAACCAAAAGTAGTCCAGATTATATCAGAAAGTTTGAAATTTCAGAAGACAGGTTCTGGAAAGATGGTCAGCCTTTCCAGATCATTGGGGGTGACTTGCATTATTTTCGGGTTCTTCCTGAGTACTGGGAAGATAGACTGTTGAGAGCAAAGGCGCTGGGATTAAATACTATTCAAACTTATGTACCCTGGAACCTGCATGAACCAAGAGCGGGTACTTTGAATTTTGAGGGTATTGCAGATTTAGTGTCATTTCTCGAACTCTGCAAGAAGCTTGGGTTTGCTTTGCCACGTATTAGTGGTGGTGCTGATGATGTAACAACAATGGAGATAATAACTGTTATTGAATATGCTTATATTGTTGAAAGATGGTGGGGAAGCCTACTTCCAAAGATAGCTCCTCTTCTTTATGGAAATGGTGGTCCAATTATAATGGTTCAGATTGAAAATGAGTATGGTTCATATGCAAGTGACAAAGCTTATCTTCATCACTTGGTCTCATTGGCTAGAAAGCACCTTGGGGATGAAGTAATTTTGTATACTACAGATGGAGGTTCTAGGGAAACTCTGGAGAAAGGAACAATTCATGGAGATGCTGTCTTTTCTGCTGTTGACTTCACTACTGGTGATAATCCTTGGCCTATATTTGAGTTACAAAAAGAGTTCAATGCCCCAGGGAAATCACCGCCACTTTCTTCGGAGTTTTACACAGGTTGGCTAACACACTGGGGGGAGAAGAATGCGCAAACTGATGCTGATTTTACAGCAGCTTCCTTAAAAACAATTTTAGAAAGAAATGGTTCTGCAGTGCTTTATATGGCACATGGAGGAACAAACTTTGGATTTTATAATGGAGCAAACACTGGTTCAGATGAGTCTGATTACAAGCCTGATCTCACTTCCTATGATTATGATGCACCAATTAGGGAATCTGGGGATGTGAGCAATGCAAAATTCAAAGCACTTAGGAAGGTTATAGAGCAATACAGTTCAGAATCTCTCCCTCCAGTTCCATCTGATATTGAAAAGAAAACATACGGATCTATTCATGTATCAAAAACCGGGAAATTGTTTGATATGATACATGATTTTGATGTGGTTGAATCTGAAAACCCAATAACAATGGAGTCGGTAGGCCAGATGTCTGGATTTTTGTTATATGTAACTGAATATGCTGCAAAGGACTATGATGGTGGAAACATTGTATCCATACCAAAGGTGCATGACAGAGCTCAAGTTTTTATATCATGTCCTTCTCAAGATGGTCATGGAAAACCAACATACGTTGGCACATTTGAAAGATGGTCGAATCAACCACTGTCCCTTCCTAATACTAAATGTCTCTCCAAAATCAGCTTATATGTGCTGGTTGAAAATATGGGACGTTTAAATTATGGGCCATATATCTTTGACATGAAGGGTATTCTATCTTCTGTTTATCTGGATGGAAGAATTCTCCGTGGATGGAAAATGTACTTGTTGCCTCTAATCAATCTGAACGAGAATCCGAAAGTCAATCCCATAATTCAGGCTACAGATTCTGGATTCATTACAATGTCAACCCGTAAAAAGTTAAAACCGAAGTCTGGTATGCTTCTAACAACTGCTTCAAATGAACCAGCATTCTATGCTGGAAAGTTTTCTATCAGCAAACAAGACAAAATAAAAGATACGTTCATATCATTGAGTGGTTGGGGTAAAGGAATTGTGACTATTAACAATTTCAATATAGGAAGATTTTGGCCGTTAAAGGGACCACAGTGCAACCTCTATGTTCCTGCTCCTATCCTTAAGCACGGGGAGAACATTGTGGTGATATTTGAGTTACAAGCGCCAAACCCTGAGCTTGTGGTACACTTGGTCGATCAGCCAGACTTTACCTGTGGTCCAAGTAAATCGAATCTGCATCAGTTGTAG

Protein Analysis

685

Amino Acids

76.54

Weight (kDa)

6.13

Isoelectric Point (pI)

37.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 65 - 141 3.5e-29 Glycosyl hydrolases family 35
Glyco_hydro_35 PF01301 162 - 374 7.5e-60 Glycosyl hydrolases family 35
BetaGal_ABD_1 PF21317 418 - 540 5.5e-23 Beta-galactosidase, first all-beta domain
BetaGal_gal-bd PF21467 586 - 648 3.7e-22 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72990 AT1G72990 AT1G72990 AT1G72990
fragaria_vesca FvH4_6g33540 FvH4_6g33540 FvH4_6g33540
malus_domestica MD09G1192500.v1.1
prunus_persica Prupe.3G048800_v2.0.a1 Prupe.3G048800_v2.0.a1
pyrus_communis pycom09g10910
rosa_chinensis RchiOBHm_Chr2g0143451
rosa_laevigata RLG00000020036
rosa_multiflora Rmu_sc0003507.1_g000021
rosa_roxburghii Rroxscaffold_2G00101830
rosa_rugosa Rorug02G0381500
rosa_samantha Rh2AG434500 Rh2BG442300 Rh2CG420400 Rh2DG452500
rosa_wichuraiana Rw2G035420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 569, 987
Acc16I TGCGCA 1 cut(s) 882
Acc36I ACCTGC 1 cut(s) 342
AccBSI CCGCTC 1 cut(s) 350
AccI GTMKAC 1 cut(s) 677
AciI CCGC 2 cut(s) 350, 824
AclWI GGATC 1 cut(s) 1186
AcoI YGGCCR 2 cut(s) 3, 1880
AcsI RAATTY 4 cut(s) 177, 361, 1091, 1590
AfaI GTAC 5 cut(s) 263, 324, 355, 1613, 1992
AfiI CCNNNNNNNGG 3 cut(s) 350, 1724, 1889
AflII CTTAAG 1 cut(s) 1928
AjnI CCWGG 3 cut(s) 5, 326, 811
AluBI AGCT 7 cut(s) 412, 536, 617, 908, 1370, 1494, 1984
AluI AGCT 7 cut(s) 412, 536, 617, 908, 1370, 1494, 1984
Alw21I GWGCWC 1 cut(s) 1372
Alw26I GTCTC 2 cut(s) 640, 1484
AlwI GGATC 1 cut(s) 1186
AlwNI CAGNNNCTG 2 cut(s) 195, 1679
AoxI GGCC 5 cut(s) 3, 776, 1273, 1535, 1880
ApeKI GCWGC 2 cut(s) 905, 1313
ApoI RAATTY 4 cut(s) 177, 361, 1091, 1590
Asp700I GAANNNNTTC 2 cut(s) 937, 1777
AspLEI GCGC 3 cut(s) 295, 883, 1972
AspS9I GGNCC 4 cut(s) 560, 1535, 1892, 2024
AsuC2I CCSGG 1 cut(s) 1201
AsuHPI GGTGA 5 cut(s) 19, 242, 776, 813, 1963
AvaII GGWCC 3 cut(s) 560, 1892, 2024
BaeI ACNNNNGTAYC 2 cut(s) 306, 339
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 1372
BarI GAAGNNNNNNTAC 2 cut(s) 1552, 1584
BbsI GAAGAC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 1372
BbvI GCAGC 2 cut(s) 917, 1300
BceAI ACGGC 1 cut(s) 1867
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciT130I CCWGG 3 cut(s) 7, 328, 813
BciVI GTATCC 1 cut(s) 1354
BcnI CCSGG 1 cut(s) 1201
BcoDI GTCTC 2 cut(s) 640, 1484
BfaI CTAG 2 cut(s) 645, 696
BfmI CTRYAG 3 cut(s) 681, 942, 1671
BfoI RGCGCY 2 cut(s) 296, 1973
BfrI CTTAAG 1 cut(s) 1928
BfuAI ACCTGC 1 cut(s) 342
BfuI GTATCC 1 cut(s) 1354
BisI GCNGC 2 cut(s) 906, 1314
BlsI GCNGC 2 cut(s) 907, 1315
BmcAI AGTACT 1 cut(s) 263
Bme1390I CCNGG 4 cut(s) 7, 328, 813, 1201
Bme18I GGWCC 3 cut(s) 560, 1892, 2024
BmgT120I GGNCC 4 cut(s) 560, 1535, 1892, 2024
BmiI GGNNCC 2 cut(s) 332, 1893
BmrFI CCNGG 4 cut(s) 7, 328, 813, 1201
BmrI ACTGGG 2 cut(s) 274, 874
BmsI GCATC 3 cut(s) 721, 880, 1042
BmuI ACTGGG 2 cut(s) 274, 874
BpiI GAAGAC 1 cut(s) 192
BpmI CTGGAG 2 cut(s) 728, 1128
Bpu10I CCTNAGC 1 cut(s) 1980
BpuEI CTTGAG 2 cut(s) 1356, 1380
BpuMI CCSGG 1 cut(s) 1201
BsaAI YACGTR 1 cut(s) 431
BsaI GGTCTC 1 cut(s) 640
BsaJI CCNNGG 6 cut(s) 326, 655, 773, 811, 812, 1597
BsaXI ACNNNNNCTCC 2 cut(s) 720, 750
Bsc4I CCNNNNNNNGG 3 cut(s) 350, 1724, 1889
Bse1I ACTGG 5 cut(s) 269, 766, 869, 1006, 1145
Bse3DI GCAATG 1 cut(s) 1090
BseBI CCWGG 3 cut(s) 7, 328, 813
BseDI CCNNGG 6 cut(s) 326, 655, 773, 811, 812, 1597
BseGI GGATG 5 cut(s) 133, 667, 1081, 1588, 1607
BseLI CCNNNNNNNGG 3 cut(s) 350, 1724, 1889
BseMI GCAATG 1 cut(s) 1090
BseMII CTCAG 2 cut(s) 249, 1971
BseNI ACTGG 5 cut(s) 269, 766, 869, 1006, 1145
BseRI GAGGAG 3 cut(s) 35, 38, 528
BseXI GCAGC 2 cut(s) 917, 1300
BseYI CCCAGC 1 cut(s) 295
BshFI GGCC 5 cut(s) 5, 778, 1275, 1537, 1882
BsiHKAI GWGCWC 1 cut(s) 1372
BsiSI CCGG 1 cut(s) 1200
BslFI GGGAC 4 cut(s) 62, 1444, 1533, 1905
BslI CCNNNNNNNGG 3 cut(s) 350, 1724, 1889
BsmAI GTCTC 2 cut(s) 640, 1484
BsmFI GGGAC 4 cut(s) 62, 1444, 1533, 1905
BsmI GAATGC 2 cut(s) 883, 1760
BsnI GGCC 5 cut(s) 5, 778, 1275, 1537, 1882
Bso31I GGTCTC 1 cut(s) 640
Bsp1286I GDGCHC 1 cut(s) 1372
Bsp143I GATC 4 cut(s) 219, 1030, 1178, 2002
BspACI CCGC 2 cut(s) 350, 824
BspANI GGCC 5 cut(s) 5, 778, 1275, 1537, 1882
BspCNI CTCAG 2 cut(s) 250, 1972
BspLI GGNNCC 2 cut(s) 332, 1893
BspMAI CTGCAG 1 cut(s) 946
BspMI ACCTGC 1 cut(s) 342
BspPI GGATC 1 cut(s) 1186
BspTI CTTAAG 1 cut(s) 1928
BspTNI GGTCTC 1 cut(s) 640
BsrBI CCGCTC 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 1090
BsrI ACTGG 5 cut(s) 269, 766, 869, 1006, 1145
BssECI CCNNGG 6 cut(s) 326, 655, 773, 811, 812, 1597
BssMI GATC 4 cut(s) 219, 1030, 1178, 2002
BssNAI GTATAC 1 cut(s) 678
BssT1I CCWWGG 2 cut(s) 655, 773
Bst1107I GTATAC 1 cut(s) 678
Bst2UI CCWGG 3 cut(s) 7, 328, 813
Bst4CI ACNGT 5 cut(s) 279, 478, 1127, 1458, 1899
Bst6I CTCTTC 2 cut(s) 5, 546
BstAFI CTTAAG 1 cut(s) 1928
BstAPI GCANNNNNTGC 1 cut(s) 1766
BstBAI YACGTR 1 cut(s) 431
BstDEI CTNAG 3 cut(s) 258, 1103, 1980
BstDSI CCRYGG 1 cut(s) 1597
BstF5I GGATG 5 cut(s) 133, 667, 1081, 1588, 1607
BstH2I RGCGCY 2 cut(s) 296, 1973
BstHHI GCGC 3 cut(s) 295, 883, 1972
BstKTI GATC 4 cut(s) 222, 1033, 1181, 2005
BstMAI GTCTC 2 cut(s) 640, 1484
BstMBI GATC 4 cut(s) 219, 1030, 1178, 2002
BstMWI GCNNNNNNNGC 1 cut(s) 1766
BstNI CCWGG 3 cut(s) 7, 328, 813
BstSCI CCNGG 4 cut(s) 5, 326, 811, 1199
BstSFI CTRYAG 3 cut(s) 681, 942, 1671
BstV1I GCAGC 2 cut(s) 917, 1300
BstV2I GAAGAC 1 cut(s) 192
BstX2I RGATCY 1 cut(s) 1178
BstXI CCANNNNNNTGG 2 cut(s) 224, 1283
BstYI RGATCY 1 cut(s) 1178
BstZ17I GTATAC 1 cut(s) 678
BsuI GTATCC 1 cut(s) 1354
BsuRI GGCC 5 cut(s) 5, 778, 1275, 1537, 1882
BtgI CCRYGG 1 cut(s) 1597
BtsCI GGATG 5 cut(s) 133, 667, 1081, 1588, 1607
BtsI GCAGTG 1 cut(s) 951
BtsIMutI CAGTG 5 cut(s) 862, 951, 999, 1454, 1904
BveI ACCTGC 1 cut(s) 342
CaiI CAGNNNCTG 2 cut(s) 195, 1679
CfoI GCGC 3 cut(s) 295, 883, 1972
Cfr13I GGNCC 4 cut(s) 560, 1535, 1892, 2024
Csp6I GTAC 5 cut(s) 262, 323, 354, 1612, 1991
CviAII CATG 9 cut(s) 338, 725, 962, 1187, 1223, 1361, 1386, 1406, 1552
CviQI GTAC 5 cut(s) 262, 323, 354, 1612, 1991
DdeI CTNAG 3 cut(s) 258, 1103, 1980
DpnI GATC 4 cut(s) 221, 1032, 1180, 2004
DpnII GATC 4 cut(s) 219, 1030, 1178, 2002
DraI TTTAAA 1 cut(s) 1527
EaeI YGGCCR 2 cut(s) 3, 1880
Eam1104I CTCTTC 2 cut(s) 5, 546
EarI CTCTTC 2 cut(s) 5, 546
Ecl136II GAGCTC 1 cut(s) 1370
Eco130I CCWWGG 2 cut(s) 655, 773
Eco24I GRGCYC 1 cut(s) 1372
Eco31I GGTCTC 1 cut(s) 640
Eco47I GGWCC 3 cut(s) 560, 1892, 2024
Eco53kI GAGCTC 1 cut(s) 1370
EcoICRI GAGCTC 1 cut(s) 1370
EcoRI GAATTC 1 cut(s) 1590
EcoRII CCWGG 3 cut(s) 5, 326, 811
EcoT14I CCWWGG 2 cut(s) 655, 773
EcoT38I GRGCYC 1 cut(s) 1372
ErhI CCWWGG 2 cut(s) 655, 773
FaeI CATG 9 cut(s) 341, 728, 965, 1190, 1226, 1364, 1389, 1409, 1555
FalI AAGNNNNNCTT 2 cut(s) 606, 638
FaqI GGGAC 4 cut(s) 62, 1444, 1533, 1905
FatI CATG 9 cut(s) 337, 724, 961, 1186, 1222, 1360, 1385, 1405, 1551
FauI CCCGC 1 cut(s) 343
FauNDI CATATG 1 cut(s) 601
FblI GTMKAC 1 cut(s) 677
Fnu4HI GCNGC 2 cut(s) 906, 1314
FokI GGATG 5 cut(s) 120, 674, 1088, 1595, 1614
FriOI GRGCYC 1 cut(s) 1372
Fsp4HI GCNGC 2 cut(s) 906, 1314
FspBI CTAG 2 cut(s) 645, 696
FspI TGCGCA 1 cut(s) 882
GlaI GCGC 3 cut(s) 294, 882, 1971
GluI GCNGC 2 cut(s) 906, 1314
GsaI CCCAGC 1 cut(s) 299
GsuI CTGGAG 2 cut(s) 728, 1128
HaeII RGCGCY 2 cut(s) 296, 1973
HaeIII GGCC 5 cut(s) 5, 778, 1275, 1537, 1882
HapII CCGG 1 cut(s) 1200
HhaI GCGC 3 cut(s) 295, 883, 1972
Hin1II CATG 9 cut(s) 341, 728, 965, 1190, 1226, 1364, 1389, 1409, 1555
Hin6I GCGC 3 cut(s) 293, 881, 1970
HinP1I GCGC 3 cut(s) 293, 881, 1970
HincII GTYRAC 2 cut(s) 751, 1698
HindII GTYRAC 2 cut(s) 751, 1698
HindIII AAGCTT 2 cut(s) 410, 615
HpaII CCGG 1 cut(s) 1200
HphI GGTGA 5 cut(s) 19, 242, 776, 813, 1963
Hpy166II GTNNAC 4 cut(s) 678, 751, 1698, 1993
Hpy8I GTNNAC 4 cut(s) 678, 751, 1698, 1993
HpyAV CCTTC 6 cut(s) 73, 108, 1102, 1401, 1472, 1549
HpyCH4III ACNGT 5 cut(s) 279, 478, 1127, 1458, 1899
HpyCH4IV ACGT 4 cut(s) 430, 1422, 1522, 1811
HpyF10VI GCNNNNNNNGC 1 cut(s) 1766
HpyF3I CTNAG 3 cut(s) 258, 1103, 1980
HpySE526I ACGT 4 cut(s) 430, 1422, 1522, 1811
Hsp92II CATG 9 cut(s) 341, 728, 965, 1190, 1226, 1364, 1389, 1409, 1555
HspAI GCGC 3 cut(s) 293, 881, 1970
Kzo9I GATC 4 cut(s) 219, 1030, 1178, 2002
LmnI GCTCC 3 cut(s) 541, 992, 1924
Lsp1109I GCAGC 2 cut(s) 917, 1300
LweI GCATC 3 cut(s) 721, 880, 1042
MaeI CTAG 2 cut(s) 645, 696
MaeII ACGT 4 cut(s) 430, 1422, 1522, 1811
MaeIII GTNAC 7 cut(s) 230, 454, 608, 789, 1300, 1846, 1962
MalI GATC 4 cut(s) 221, 1032, 1180, 2004
MbiI CCGCTC 1 cut(s) 350
MboI GATC 4 cut(s) 219, 1030, 1178, 2002
MflI RGATCY 1 cut(s) 1178
MhlI GDGCHC 1 cut(s) 1372
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 18, 1022, 1274
MmeI TCCRAC 1 cut(s) 161
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 937, 1777
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 302, 914, 1526, 1712, 1854, 1886, 1929
MslI CAYNNNNRTG 3 cut(s) 729, 1691, 1949
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 1928
MspI CCGG 1 cut(s) 1200
MspR9I CCNGG 4 cut(s) 7, 328, 813, 1201
Mva1269I GAATGC 2 cut(s) 883, 1760
MvaI CCWGG 3 cut(s) 7, 328, 813
MwoI GCNNNNNNNGC 1 cut(s) 1766
NciI CCSGG 1 cut(s) 1201
NdeI CATATG 1 cut(s) 601
NdeII GATC 4 cut(s) 219, 1030, 1178, 2002
NlaIII CATG 9 cut(s) 341, 728, 965, 1190, 1226, 1364, 1389, 1409, 1555
NlaIV GGNNCC 2 cut(s) 332, 1893
NmuCI GTSAC 3 cut(s) 230, 608, 1846
NsbI TGCGCA 1 cut(s) 882
PasI CCCWGGG 1 cut(s) 812
PctI GAATGC 2 cut(s) 883, 1760
PdmI GAANNNNTTC 2 cut(s) 937, 1777
PfeI GAWTC 8 cut(s) 1067, 1133, 1241, 1447, 1642, 1676, 1683, 2038
PkrI GCNGC 2 cut(s) 907, 1315
PleI GAGTC 3 cut(s) 18, 1021, 1273
PpsI GAGTC 3 cut(s) 18, 1021, 1273
Ppu21I YACGTR 1 cut(s) 431
PsiI TTATAA 2 cut(s) 569, 987
Psp124BI GAGCTC 1 cut(s) 1372
Psp6I CCWGG 3 cut(s) 5, 326, 811
PspFI CCCAGC 1 cut(s) 295
PspGI CCWGG 3 cut(s) 5, 326, 811
PspN4I GGNNCC 2 cut(s) 332, 1893
PspPI GGNCC 4 cut(s) 560, 1535, 1892, 2024
PstI CTGCAG 1 cut(s) 946
PstNI CAGNNNCTG 2 cut(s) 195, 1679
PsuI RGATCY 1 cut(s) 1178
RsaI GTAC 5 cut(s) 263, 324, 355, 1613, 1992
RsaNI GTAC 5 cut(s) 262, 323, 354, 1612, 1991
RseI CAYNNNNRTG 3 cut(s) 729, 1691, 1949
SacI GAGCTC 1 cut(s) 1372
SaqAI TTAA 7 cut(s) 302, 914, 1526, 1712, 1854, 1886, 1929
SatI GCNGC 2 cut(s) 906, 1314
Sau3AI GATC 4 cut(s) 219, 1030, 1178, 2002
Sau96I GGNCC 4 cut(s) 560, 1535, 1892, 2024
ScaI AGTACT 1 cut(s) 263
SchI GAGTC 3 cut(s) 18, 1022, 1274
ScrFI CCNGG 4 cut(s) 7, 328, 813, 1201
SduI GDGCHC 1 cut(s) 1372
SfaNI GCATC 3 cut(s) 721, 880, 1042
SfcI CTRYAG 3 cut(s) 681, 942, 1671
SinI GGWCC 3 cut(s) 560, 1892, 2024
SmiMI CAYNNNNRTG 3 cut(s) 729, 1691, 1949
SmlI CTYRAG 3 cut(s) 1371, 1395, 1928
SmoI CTYRAG 3 cut(s) 1371, 1395, 1928
SsiI CCGC 2 cut(s) 350, 824
SspMI CTAG 2 cut(s) 645, 696
SstI GAGCTC 1 cut(s) 1372
StyD4I CCNGG 4 cut(s) 5, 326, 811, 1199
StyI CCWWGG 2 cut(s) 655, 773
TaaI ACNGT 5 cut(s) 279, 478, 1127, 1458, 1899
TaiI ACGT 4 cut(s) 433, 1425, 1525, 1814
TaqI TCGA 4 cut(s) 396, 1445, 2001, 2036
TatI WGTACW 2 cut(s) 261, 1611
TfiI GAWTC 8 cut(s) 1067, 1133, 1241, 1447, 1642, 1676, 1683, 2038
Tru1I TTAA 7 cut(s) 302, 914, 1526, 1712, 1854, 1886, 1929
Tru9I TTAA 7 cut(s) 302, 914, 1526, 1712, 1854, 1886, 1929
TscAI CASTG 5 cut(s) 869, 951, 1006, 1461, 1904
TseFI GTSAC 3 cut(s) 230, 608, 1846
TseI GCWGC 2 cut(s) 905, 1313
Tsp45I GTSAC 3 cut(s) 230, 608, 1846
TspGWI ACGGA 2 cut(s) 1191, 1586
TspRI CASTG 5 cut(s) 869, 951, 1006, 1461, 1904
Vha464I CTTAAG 1 cut(s) 1928
VpaK11BI GGWCC 3 cut(s) 560, 1892, 2024
XapI RAATTY 4 cut(s) 177, 361, 1091, 1590
XmiI GTMKAC 1 cut(s) 677
XmnI GAANNNNTTC 2 cut(s) 937, 1777
XspI CTAG 2 cut(s) 645, 696
ZrmI AGTACT 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.