pycom09g10910

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0010424
Physical Location & Seq
Forward (+)
9021324 .. 9024598
3275 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g10910.3

Sequence Viewer

Length: 1473 bp
ATGGTTCAGATTGAAAACGAATTTGGATCATATGGGAACGACAAAGCTTATCTTCATCACCTGGTGTCAGTGGCTAGAAAGCACCTTGGGGAAGATGCAATTTTGTATACTACAGATGGAGGTTCTAGGGAAACACTAGAGAAAGGAACAATTCGTGGAGATGCTGTCTTTTCAGCTGTTGATTTCACTACTGGTGATGATCCTTGGCCTATATTTGAGTTGCAAAGGGAGTTCAATGCCCCCGGGAAATCACCACCACTTTCTTCAGAGTTTTACACAGGCTGGCTTACACATTGGGGAGAGAAGAATGCGCAGACTGATGCTGCTTTTACAGCAGCTGCCTTGAAAAAGATTTTGGAAAGAAATGGTTCTGCAGTGCTTTATATGGCACATGGGGGAACAAACTTTGGGTTTTATAATGGAGCAAATACCGGTTCAGACGAGTCTGATTACAAGCCCGACCTCACTTCCTATGATTATGATGCACCGATCAGGGAATCTGGGGATGTAAACAATGCAAAATTCAAAGCAATTAGGAGGGTCGTAGAGCAACACAGTCTAGGATCTATCCCTGCAGTTCCAGCCGATAATGAGAAGAAAGCATATGGATCCATTCACGTAACTAAAACAGGGACTTTATTTGATATGATCCATTACTTTGATGTGGTTGAATCCGAGAGCCCAATATCTATGGAGTCAGCGGGACAGATGTTTGGATTTATGTTATACGTGACTGAGTATGCTGCAAAAGACAATGGTGGCGGAGTTGTATCCATACCAAAGGTGCACGACAGAGCCCAAGTTTTTGTAATGTGCTCTTCTGAAGATGGTCATGGAAGACCAAAATATGTTGGCACTTTTGAAAGATGGTCAAATCAACCTCTAACCCTTCCTAATACGAAATGCCGCTCTAACATCAGCTTATTTGTGTTGGTTGAAAACATGGGCCGTCTAAATTATGGACCATATATCTTTGACAGGAAGGGTATTCTGTCTCCTGTTTATCTTGATGGGAGAATTCTCCGAAGATGGAAAATGCATTTGATACCTATAGGAAATCTGAATGAGGTTCCTAAAATCAATCCCATAATTCAGGCTTCACATTCTGGAGTCATTACTATGTCAGCGCGCAAAAGATTAAAACCGAAAAAGTCTGGGAATGATTTGAACGTGCCAGCATTCTATGCTGGATATATTTCTATCAGCAAAGAAGACGAAATCAAAGATACATTCATATCATTGAGTGGCTGGGGTAAAGGGATAGTAACTATTAACAATTTCAACATCGGAAGATTTTGGCCGTTAAAGGGACCGCAGTGTAACCTCTATGTTCCTGCTCCTGTCCTTCGTCGTGGGAATAATGTTGTGGTCATATTTGAGTTAGAGTCCCCAAACCCGGAGCTTGTGGTACGCTTTGTTGATCAGCCAGACTTCACTTGTGGTCCAGGGAAATCGAATGTGCATCAGCTTTAG

Protein Analysis

491

Amino Acids

54.39

Weight (kDa)

6.68

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72990 AT1G72990 AT1G72990 AT1G72990
fragaria_vesca FvH4_6g33540 FvH4_6g33540 FvH4_6g33540
malus_domestica MD09G1192500.v1.1
prunus_persica Prupe.3G048800_v2.0.a1 Prupe.3G048800_v2.0.a1
pyrus_communis pycom09g10910
rosa_chinensis RchiOBHm_Chr2g0143451
rosa_laevigata RLG00000020036
rosa_multiflora Rmu_sc0003507.1_g000021
rosa_roxburghii Rroxscaffold_2G00101830
rosa_rugosa Rorug02G0381500
rosa_samantha Rh2AG434500 Rh2BG442300 Rh2CG420400 Rh2DG452500
rosa_wichuraiana Rw2G035420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 417
Acc16I TGCGCA 1 cut(s) 312
AccBSI CCGCTC 1 cut(s) 909
AccI GTMKAC 1 cut(s) 107
AccII CGCG 1 cut(s) 1129
AciI CCGC 4 cut(s) 701, 762, 907, 1313
AclWI GGATC 6 cut(s) 34, 194, 571, 603, 616, 643
AcoI YGGCCR 1 cut(s) 1298
AcsI RAATTY 3 cut(s) 20, 521, 1017
AcuI CTGAAG 2 cut(s) 249, 843
AdeI CACNNNGTG 1 cut(s) 64
AfaI GTAC 1 cut(s) 1410
AfiI CCNNNNNNNGG 2 cut(s) 1307, 1396
AgeI ACCGGT 1 cut(s) 431
AgsI TTSAA 9 cut(s) 14, 235, 346, 526, 671, 863, 938, 1168, 1282
AjnI CCWGG 2 cut(s) 60, 1444
AjuI GAANNNNNNNTTGG 3 cut(s) 38, 338, 370
AluBI AGCT 6 cut(s) 47, 176, 338, 921, 1402, 1468
AluI AGCT 6 cut(s) 47, 176, 338, 921, 1402, 1468
Alw21I GWGCWC 2 cut(s) 789, 818
Alw26I GTCTC 1 cut(s) 999
Alw44I GTGCAC 1 cut(s) 785
AlwI GGATC 6 cut(s) 34, 194, 571, 603, 616, 643
AlwNI CAGNNNCTG 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 3 cut(s) 206, 946, 1298
ApaLI GTGCAC 1 cut(s) 785
ApeKI GCWGC 4 cut(s) 323, 335, 338, 743
ApoI RAATTY 3 cut(s) 20, 521, 1017
AsiGI ACCGGT 1 cut(s) 431
Asp700I GAANNNNTTC 1 cut(s) 367
AspLEI GCGC 3 cut(s) 313, 1129, 1131
AspS9I GGNCC 4 cut(s) 946, 962, 1310, 1442
AsuC2I CCSGG 3 cut(s) 243, 244, 1397
AsuHPI GGTGA 3 cut(s) 50, 206, 243
AvaI CYCGRG 1 cut(s) 242
AvaII GGWCC 3 cut(s) 962, 1310, 1442
BaeGI GKGCMC 1 cut(s) 789
BamHI GGATCC 1 cut(s) 608
BanII GRGCYC 2 cut(s) 683, 799
BbsI GAAGAC 2 cut(s) 844, 1218
Bbv12I GWGCWC 2 cut(s) 789, 818
BbvI GCAGC 4 cut(s) 310, 325, 347, 730
BccI CCATC 5 cut(s) 110, 821, 861, 1004, 1023
BceAI ACGGC 2 cut(s) 933, 1285
BciT130I CCWGG 2 cut(s) 62, 1446
BciVI GTATCC 1 cut(s) 781
BclI TGATCA 1 cut(s) 1420
BcnI CCSGG 3 cut(s) 243, 244, 1397
BcoDI GTCTC 1 cut(s) 999
BfaI CTAG 4 cut(s) 75, 126, 137, 560
BfmI CTRYAG 4 cut(s) 111, 372, 573, 1050
BfuI GTATCC 1 cut(s) 781
BisI GCNGC 5 cut(s) 324, 336, 339, 744, 907
BlsI GCNGC 5 cut(s) 325, 337, 340, 745, 908
Bme1390I CCNGG 5 cut(s) 62, 243, 244, 1397, 1446
Bme18I GGWCC 3 cut(s) 962, 1310, 1442
BmeT110I CYCGRG 1 cut(s) 242
BmgT120I GGNCC 4 cut(s) 946, 962, 1310, 1442
BmiI GGNNCC 3 cut(s) 610, 1071, 1311
BmrFI CCNGG 5 cut(s) 62, 243, 244, 1397, 1446
BmsI GCATC 4 cut(s) 85, 151, 310, 472
BpiI GAAGAC 2 cut(s) 844, 1218
BpmI CTGGAG 1 cut(s) 1128
BpuMI CCSGG 3 cut(s) 243, 244, 1397
BsaAI YACGTR 2 cut(s) 619, 730
BsaJI CCNNGG 5 cut(s) 85, 203, 241, 242, 1445
BsaWI WCCGGW 1 cut(s) 431
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 2 cut(s) 1307, 1396
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 1 cut(s) 196
BseBI CCWGG 2 cut(s) 62, 1446
BseDI CCNNGG 5 cut(s) 85, 203, 241, 242, 1445
BseGI GGATG 1 cut(s) 511
BseLI CCNNNNNNNGG 2 cut(s) 1307, 1396
BseMII CTCAG 1 cut(s) 726
BseNI ACTGG 1 cut(s) 196
BsePI GCGCGC 1 cut(s) 1127
BseSI GKGCMC 1 cut(s) 789
BseXI GCAGC 4 cut(s) 310, 325, 347, 730
BseYI CCCAGC 1 cut(s) 1248
Bsh1236I CGCG 1 cut(s) 1129
BshFI GGCC 3 cut(s) 208, 948, 1300
BshTI ACCGGT 1 cut(s) 431
BsiHKAI GWGCWC 2 cut(s) 789, 818
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 3 cut(s) 243, 432, 1397
BslFI GGGAC 4 cut(s) 646, 717, 1323, 1372
BslI CCNNNNNNNGG 2 cut(s) 1307, 1396
BsmAI GTCTC 1 cut(s) 999
BsmFI GGGAC 4 cut(s) 646, 717, 1323, 1372
BsmI GAATGC 2 cut(s) 313, 1178
BsnI GGCC 3 cut(s) 208, 948, 1300
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 4 cut(s) 683, 789, 799, 818
Bsp143I GATC 7 cut(s) 26, 199, 489, 563, 608, 648, 1420
BspACI CCGC 4 cut(s) 701, 762, 907, 1313
BspANI GGCC 3 cut(s) 208, 948, 1300
BspCNI CTCAG 1 cut(s) 727
BspFNI CGCG 1 cut(s) 1129
BspLI GGNNCC 3 cut(s) 610, 1071, 1311
BspMAI CTGCAG 2 cut(s) 376, 577
BspPI GGATC 6 cut(s) 34, 194, 571, 603, 616, 643
BspQI GCTCTTC 1 cut(s) 823
BsrBI CCGCTC 1 cut(s) 909
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 1 cut(s) 196
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 5 cut(s) 85, 203, 241, 242, 1445
BssHII GCGCGC 1 cut(s) 1127
BssMI GATC 7 cut(s) 26, 199, 489, 563, 608, 648, 1420
BssNAI GTATAC 1 cut(s) 108
BssT1I CCWWGG 2 cut(s) 85, 203
Bst1107I GTATAC 1 cut(s) 108
Bst2UI CCWGG 2 cut(s) 62, 1446
Bst4CI ACNGT 1 cut(s) 557
Bst6I CTCTTC 1 cut(s) 823
BstAPI GCANNNNNTGC 1 cut(s) 1184
BstBAI YACGTR 2 cut(s) 619, 730
BstC8I GCNNGC 3 cut(s) 284, 1129, 1176
BstDEI CTNAG 1 cut(s) 735
BstF5I GGATG 1 cut(s) 511
BstFNI CGCG 1 cut(s) 1129
BstHHI GCGC 3 cut(s) 313, 1129, 1131
BstKTI GATC 7 cut(s) 29, 202, 492, 566, 611, 651, 1423
BstMAI GTCTC 1 cut(s) 999
BstMBI GATC 7 cut(s) 26, 199, 489, 563, 608, 648, 1420
BstMWI GCNNNNNNNGC 3 cut(s) 332, 581, 1184
BstNI CCWGG 2 cut(s) 62, 1446
BstSCI CCNGG 5 cut(s) 60, 241, 242, 1395, 1444
BstSFI CTRYAG 4 cut(s) 111, 372, 573, 1050
BstSLI GKGCMC 1 cut(s) 789
BstUI CGCG 1 cut(s) 1129
BstV1I GCAGC 4 cut(s) 310, 325, 347, 730
BstV2I GAAGAC 2 cut(s) 844, 1218
BstX2I RGATCY 2 cut(s) 563, 608
BstYI RGATCY 2 cut(s) 563, 608
BstZ17I GTATAC 1 cut(s) 108
BsuI GTATCC 1 cut(s) 781
BsuRI GGCC 3 cut(s) 208, 948, 1300
BtsCI GGATG 1 cut(s) 511
BtsI GCAGTG 2 cut(s) 381, 1322
BtsIMutI CAGTG 3 cut(s) 75, 381, 1322
Cac8I GCNNGC 3 cut(s) 284, 1129, 1176
CaiI CAGNNNCTG 1 cut(s) 338
CfoI GCGC 3 cut(s) 313, 1129, 1131
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 4 cut(s) 946, 962, 1310, 1442
Cfr9I CCCGGG 1 cut(s) 242
CsiI ACCWGGT 1 cut(s) 60
Csp6I GTAC 1 cut(s) 1409
CspAI ACCGGT 1 cut(s) 431
CviAII CATG 3 cut(s) 392, 833, 943
CviQI GTAC 1 cut(s) 1409
DdeI CTNAG 1 cut(s) 735
DpnI GATC 7 cut(s) 28, 201, 491, 565, 610, 650, 1422
DpnII GATC 7 cut(s) 26, 199, 489, 563, 608, 648, 1420
DraIII CACNNNGTG 1 cut(s) 64
EaeI YGGCCR 1 cut(s) 1298
Eam1104I CTCTTC 1 cut(s) 823
EarI CTCTTC 1 cut(s) 823
EciI GGCGGA 1 cut(s) 777
Eco130I CCWWGG 2 cut(s) 85, 203
Eco24I GRGCYC 2 cut(s) 683, 799
Eco47I GGWCC 3 cut(s) 962, 1310, 1442
Eco57I CTGAAG 2 cut(s) 249, 843
Eco88I CYCGRG 1 cut(s) 242
EcoRI GAATTC 1 cut(s) 1017
EcoRII CCWGG 2 cut(s) 60, 1444
EcoT14I CCWWGG 2 cut(s) 85, 203
EcoT22I ATGCAT 1 cut(s) 1041
EcoT38I GRGCYC 2 cut(s) 683, 799
ErhI CCWWGG 2 cut(s) 85, 203
FaeI CATG 3 cut(s) 395, 836, 946
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FaqI GGGAC 4 cut(s) 646, 717, 1323, 1372
FatI CATG 3 cut(s) 391, 832, 942
FauI CCCGC 1 cut(s) 694
FauNDI CATATG 2 cut(s) 31, 604
FbaI TGATCA 1 cut(s) 1420
FblI GTMKAC 1 cut(s) 107
Fnu4HI GCNGC 5 cut(s) 324, 336, 339, 744, 907
FokI GGATG 1 cut(s) 518
FriOI GRGCYC 2 cut(s) 683, 799
Fsp4HI GCNGC 5 cut(s) 324, 336, 339, 744, 907
FspBI CTAG 4 cut(s) 75, 126, 137, 560
FspI TGCGCA 1 cut(s) 312
GlaI GCGC 3 cut(s) 312, 1128, 1130
GluI GCNGC 5 cut(s) 324, 336, 339, 744, 907
GsaI CCCAGC 1 cut(s) 1252
GsuI CTGGAG 1 cut(s) 1128
HaeIII GGCC 3 cut(s) 208, 948, 1300
HapII CCGG 3 cut(s) 243, 432, 1397
HhaI GCGC 3 cut(s) 313, 1129, 1131
Hin1II CATG 3 cut(s) 395, 836, 946
Hin6I GCGC 3 cut(s) 311, 1127, 1129
HinP1I GCGC 3 cut(s) 311, 1127, 1129
HindIII AAGCTT 1 cut(s) 45
HinfI GANTC 6 cut(s) 443, 497, 671, 695, 1110, 1385
HpaII CCGG 3 cut(s) 243, 432, 1397
HphI GGTGA 3 cut(s) 50, 206, 243
Hpy166II GTNNAC 3 cut(s) 108, 511, 787
Hpy188I TCNGA 9 cut(s) 9, 268, 439, 448, 676, 823, 1025, 1062, 1289
Hpy188III TCNNGA 2 cut(s) 1007, 1107
Hpy8I GTNNAC 3 cut(s) 108, 511, 787
Hpy99I CGWCG 1 cut(s) 1353
HpyAV CCTTC 3 cut(s) 899, 976, 1355
HpyCH4III ACNGT 1 cut(s) 557
HpyCH4IV ACGT 3 cut(s) 618, 729, 1170
HpyF10VI GCNNNNNNNGC 3 cut(s) 332, 581, 1184
HpyF3I CTNAG 1 cut(s) 735
HpySE526I ACGT 3 cut(s) 618, 729, 1170
Hsp92II CATG 3 cut(s) 395, 836, 946
HspAI GCGC 3 cut(s) 311, 1127, 1129
Ksp22I TGATCA 1 cut(s) 1420
Kzo9I GATC 7 cut(s) 26, 199, 489, 563, 608, 648, 1420
LguI GCTCTTC 1 cut(s) 823
LmnI GCTCC 3 cut(s) 422, 1342, 1399
Lsp1109I GCAGC 4 cut(s) 310, 325, 347, 730
LweI GCATC 4 cut(s) 85, 151, 310, 472
MabI ACCWGGT 1 cut(s) 60
MaeI CTAG 4 cut(s) 75, 126, 137, 560
MaeII ACGT 3 cut(s) 618, 729, 1170
MaeIII GTNAC 4 cut(s) 619, 730, 1264, 1319
MalI GATC 7 cut(s) 28, 201, 491, 565, 610, 650, 1422
MbiI CCGCTC 1 cut(s) 909
MboI GATC 7 cut(s) 26, 199, 489, 563, 608, 648, 1420
MflI RGATCY 2 cut(s) 563, 608
MhlI GDGCHC 4 cut(s) 683, 789, 799, 818
MluCI AATT 9 cut(s) 20, 99, 150, 521, 531, 955, 1017, 1089, 1276
MlyI GAGTC 4 cut(s) 452, 704, 1119, 1394
MnlI CCTC 6 cut(s) 113, 473, 531, 891, 1060, 1334
Mph1103I ATGCAT 1 cut(s) 1041
MroXI GAANNNNTTC 1 cut(s) 367
MseI TTAA 3 cut(s) 1139, 1272, 1304
MslI CAYNNNNRTG 1 cut(s) 1118
MspA1I CMGCKG 3 cut(s) 176, 338, 701
MspI CCGG 3 cut(s) 243, 432, 1397
MspR9I CCNGG 5 cut(s) 62, 243, 244, 1397, 1446
Mva1269I GAATGC 2 cut(s) 313, 1178
MvaI CCWGG 2 cut(s) 62, 1446
MvnI CGCG 1 cut(s) 1129
MwoI GCNNNNNNNGC 3 cut(s) 332, 581, 1184
NciI CCSGG 3 cut(s) 243, 244, 1397
NdeI CATATG 2 cut(s) 31, 604
NdeII GATC 7 cut(s) 26, 199, 489, 563, 608, 648, 1420
NlaIII CATG 3 cut(s) 395, 836, 946
NlaIV GGNNCC 3 cut(s) 610, 1071, 1311
NmuCI GTSAC 1 cut(s) 730
NsbI TGCGCA 1 cut(s) 312
NsiI ATGCAT 1 cut(s) 1041
PauI GCGCGC 1 cut(s) 1127
PciSI GCTCTTC 1 cut(s) 823
PctI GAATGC 2 cut(s) 313, 1178
PdmI GAANNNNTTC 1 cut(s) 367
PfeI GAWTC 2 cut(s) 497, 671
PinAI ACCGGT 1 cut(s) 431
PkrI GCNGC 5 cut(s) 325, 337, 340, 745, 908
PleI GAGTC 4 cut(s) 451, 703, 1118, 1393
PpsI GAGTC 4 cut(s) 451, 703, 1118, 1393
Ppu21I YACGTR 2 cut(s) 619, 730
PsiI TTATAA 1 cut(s) 417
Psp6I CCWGG 2 cut(s) 60, 1444
PspFI CCCAGC 1 cut(s) 1248
PspGI CCWGG 2 cut(s) 60, 1444
PspN4I GGNNCC 3 cut(s) 610, 1071, 1311
PspPI GGNCC 4 cut(s) 946, 962, 1310, 1442
PstI CTGCAG 2 cut(s) 376, 577
PstNI CAGNNNCTG 1 cut(s) 338
PsuI RGATCY 2 cut(s) 563, 608
PteI GCGCGC 1 cut(s) 1127
PvuII CAGCTG 2 cut(s) 176, 338
RsaI GTAC 1 cut(s) 1410
RsaNI GTAC 1 cut(s) 1409
RseI CAYNNNNRTG 1 cut(s) 1118
SapI GCTCTTC 1 cut(s) 823
SaqAI TTAA 3 cut(s) 1139, 1272, 1304
SatI GCNGC 5 cut(s) 324, 336, 339, 744, 907
Sau3AI GATC 7 cut(s) 26, 199, 489, 563, 608, 648, 1420
Sau96I GGNCC 4 cut(s) 946, 962, 1310, 1442
SchI GAGTC 4 cut(s) 452, 704, 1119, 1394
ScrFI CCNGG 5 cut(s) 62, 243, 244, 1397, 1446
SduI GDGCHC 4 cut(s) 683, 789, 799, 818
SexAI ACCWGGT 1 cut(s) 60
SfaNI GCATC 4 cut(s) 85, 151, 310, 472
SfcI CTRYAG 4 cut(s) 111, 372, 573, 1050
SinI GGWCC 3 cut(s) 962, 1310, 1442
SmaI CCCGGG 1 cut(s) 244
SmiMI CAYNNNNRTG 1 cut(s) 1118
Sse9I AATT 9 cut(s) 20, 99, 150, 521, 531, 955, 1017, 1089, 1276
SsiI CCGC 4 cut(s) 701, 762, 907, 1313
SspMI CTAG 4 cut(s) 75, 126, 137, 560
StyD4I CCNGG 5 cut(s) 60, 241, 242, 1395, 1444
StyI CCWWGG 2 cut(s) 85, 203
TaaI ACNGT 1 cut(s) 557
TaiI ACGT 3 cut(s) 621, 732, 1173
TaqI TCGA 1 cut(s) 1454
TasI AATT 9 cut(s) 20, 99, 150, 521, 531, 955, 1017, 1089, 1276
TauI GCSGC 1 cut(s) 909
TfiI GAWTC 2 cut(s) 497, 671
Tru1I TTAA 3 cut(s) 1139, 1272, 1304
Tru9I TTAA 3 cut(s) 1139, 1272, 1304
TscAI CASTG 3 cut(s) 75, 381, 1322
TseFI GTSAC 1 cut(s) 730
TseI GCWGC 4 cut(s) 323, 335, 338, 743
Tsp45I GTSAC 1 cut(s) 730
TspDTI ATGAA 2 cut(s) 44, 1222
TspMI CCCGGG 1 cut(s) 242
TspRI CASTG 3 cut(s) 75, 381, 1322
VneI GTGCAC 1 cut(s) 785
VpaK11BI GGWCC 3 cut(s) 962, 1310, 1442
XapI RAATTY 3 cut(s) 20, 521, 1017
XmaI CCCGGG 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 107
XmnI GAANNNNTTC 1 cut(s) 367
XspI CTAG 4 cut(s) 75, 126, 137, 560
Zsp2I ATGCAT 1 cut(s) 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.