Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0010424

Physical Location & Seq

Genomic Coordinates Forward (+)

23761064 .. 23769958

Gene Structure 8895 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00101830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2388 bp

ATGTCGGCCTCCACCGCCGCCGCTCCGTCCCACCGTCGGACCACCGAGAGGATGACACAATCTAACTCCACGATCCCAACCCTCCGGGCTTCGCCGATTTTGTTCGTCGGATGTCCGGAAGCTCTCCACGCCGAATCGGGTCCGGGCTTCGCCGGTTTTGTTCGTCGGATGTCCGGAAGCTCCGAAGCTCCACGCCGAATCGTCCCGGATAGTAGTTTTCGGTCCATCGTCGATTCCTTCGAAGGTCAACCCCGCCTCGCCGAGCTTCTCACCGACGAGGATCGTCGGCTTGGCGTCGAGCCCGGCGGCGGAGAGCACGATGACGATTTTCGGCGCGGCGATGGCGAGAGCGGGCGTCGTGGGGGACCCGCGGAGGGAGGGAGGGAGGGAGAGAGAAATCCGATGTCGTGCGGAGAGAGAGAGAGGGAGAGAGAGAGAGAGAAATCGAGTCCAGATTATGTCAGAAAGTTTGAAATTTCAGAAGACAGGTTCTGGAAAGATGGTCAGCCTTTCCAGATCATTGGGGGTGACTTGCATTATTTTCGGGTTCTTCCTGAGTACTGGGAAGATAGGCTGTTGAGAGCAGAGGCATTGGGATTAAATACTATTCAAACTTATGTACCCTGGAACCTGCATGAACCAAGAGCTGGTACTTTGAATTTTGAGGGTATTGCAGATTTAGTATCATTTCTCAAACTCTGCCAGAAGCTTGGGTTTGCTGTTATGCTTAGACCTGGGCCATATATATGTGGAGAGTGGGATTTGGGTGGTTTCCCTTCTTGGTTACTTGCCATCAAGCCAGCTCTCACACTAAGATCATCTGACCCAGCTTTCCTTCAATTGGTCGAAAGATGGTGGGGAAGCCTACTTCCAAAGATAGCTCCTCTTCTTTATGGAAATGGTGGTCCAATTATAATGGTTCAGATTGAAAATGAGTATGGTTCATATGCAAGTGACAAAGCTTATCTTCATCACTTGGTCTCATTGGCTAGAAAGCACCTTGGGGATGAAGTAATTTTGTATACTACAGATGGAGGTTCTAGGGAAAGTCTGGAGAAAGGAACAATTCGTGGAGATGCTGTCTTTTCTGCTGTTGACTTCACTACTGGTGATAATCCTTGGCCTATATTTGAGTTACAAAAAGAGTTCAATGCCCCAGGGAAATCACCGCCACTTTCTTCGGAGTTTTACACAGGTTGGCTAACACACTGGGGGGAGAAGAATGCGCAAACTGATGCTGATTTTACAGCAGCTTCCTTAAAAAGAATTTTAGAAAGAAATGGTTCTGCAGTGCTTTATATGGCACATGGAGGAACAAACTTTGGATTTTATAATGGAGCAAACACTGGTTCAGATGAGTCTGATTACATGCCTGATCTCACTTCCTATGATTATGATGCACCAATTAGGGAATCTGGGGATGTGAGCAATGCAAAATTCAAAGCACTTAGGAAGGTTATAGAGCAATACAGTTCAGAATCTCTCCCTCCAGTTCCATCCGATATTGAAAAGAAAACATACGGATCTATTCATGTATCAAAAACCGGGAATTTGTTTGATATGATACATGATTTTGATGTGGTTGAATCTGAAAACCCAATAACAATGGAGTCGGTAGGCCAGATGTCTGGATTTTTGTTATATGTAACTGAATATGCTGCAAAGGACTATGATGGTGGAAACATTGTATCCATACCAAAGGTGCATGACAGAGCTCAAGTTTTTATATCATGTCCTTCTCAAGATGGTCATGTAAAACCAACATACGTTGGTACATTTGAAAGATGGTCGAATCAACCACTGTCCCTTCCTAATACTAAATGTCTCTCCAAAATCAGCTTATATGTGCTGGTTGAAAATATGGGACGTTTAAATTATGGGCCATATATCTTTGACATGAAGGGTATTCTATCTTCTGTTTATCTGGATGGAAGAATTCTCCGTGGATGGAAAATGTACTTGTTGCCTCTAATCAACCTGAACGAGAATCCGAAAGTCAATCCCATAATTCAGGCTACAGATTCTGGATTCATTACAATGTCAACCCGTAAAAAGTTAAAACCGAAGTCTGCAACTGCTTCAAATGAACCAGCATTCTATGCTGGAAAGTTTTCTATCAGCAAACAAGACAAAATAAAAGATACGTTCATTTCATTGAGTGGTTGGGGTAAAGGAATTGTGACTATTAACAATTTCAATATAGGAAGATTTTGGCCGTTAAAGGGACCACAGTGCAACCTCTATGTTCCTGCTCCTATCCTTAAGCATGGGGAGAACATTGTGGTGATATTTGAGTTACAAGCACCAAACCCTGAGCTTGTGGTACACTTGGTCGATCAGCCAGACTTTACCTGTGGTCCAAGTAAATCGAATCTGCATCAGTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00052 ko00511 ko00531 ko00600 ko00604 ko01100 ko04142 map00052 map00511 map00531 map00600 map00604 map01100 map04142

Pfam Domains

Protein Families

BetaGal_dom4_5 Glyco_hydro_35

Protein Analysis

795

Amino Acids

88.47

Weight (kDa)

5.81

Isoelectric Point (pI)

46.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	164 - 489	1e-117	Glycosyl hydrolases family 35
Glyco_hydro_42	PF02449	183 - 321	7.8e-06	Beta-galactosidase
BetaGal_ABD_1	PF21317	533 - 655	4.9e-23	Beta-galactosidase, first all-beta domain
BetaGal_gal-bd	PF21467	697 - 759	4.5e-22	Beta-galactosidase, galactose-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010424)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G72990 AT1G72990 AT1G72990 AT1G72990
fragaria_vesca	FvH4_6g33540 FvH4_6g33540 FvH4_6g33540
malus_domestica	MD09G1192500.v1.1
prunus_persica	Prupe.3G048800_v2.0.a1 Prupe.3G048800_v2.0.a1
pyrus_communis	pycom09g10910
rosa_chinensis	RchiOBHm_Chr2g0143451
rosa_laevigata	RLG00000020036
rosa_multiflora	Rmu_sc0003507.1_g000021
rosa_roxburghii	Rroxscaffold_2G00101830
rosa_rugosa	Rorug02G0381500
rosa_samantha	Rh2AG434500 Rh2BG442300 Rh2CG420400 Rh2DG452500
rosa_wichuraiana	Rw2G035420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	914, 1332
Acc16I	TGCGCA	1 cut(s)	1227
Acc36I	ACCTGC	1 cut(s)	639
AccB7I	CCANNNNNTGG	1 cut(s)	647
AccBSI	CCGCTC	2 cut(s)	23, 351
AccI	GTMKAC	1 cut(s)	1022
AccII	CGCG	2 cut(s)	336, 371
AccIII	TCCGGA	2 cut(s)	115, 173
AclWI	GGATC	3 cut(s)	67, 288, 1531
AcoI	YGGCCR	1 cut(s)	2213
AcsI	RAATTY	6 cut(s)	474, 658, 1266, 1436, 1549, 1935
AcyI	GRCGYC	2 cut(s)	294, 355
AfaI	GTAC	6 cut(s)	560, 621, 652, 1774, 1958, 2325
AfiI	CCNNNNNNNGG	7 cut(s)	36, 48, 308, 374, 647, 841, 2222
AflII	CTTAAG	1 cut(s)	2261
AjnI	CCWGG	3 cut(s)	623, 733, 1156
Alw21I	GWGCWC	2 cut(s)	318, 1717
Alw26I	GTCTC	2 cut(s)	985, 1829
AlwI	GGATC	3 cut(s)	67, 288, 1531
AlwNI	CAGNNNCTG	2 cut(s)	492, 2024
Aor13HI	TCCGGA	2 cut(s)	115, 173
AoxI	GGCC	6 cut(s)	6, 737, 1121, 1618, 1880, 2213
ApeKI	GCWGC	2 cut(s)	1250, 1658
ApoI	RAATTY	6 cut(s)	474, 658, 1266, 1436, 1549, 1935
Asp700I	GAANNNNTTC	2 cut(s)	1282, 2110
AspLEI	GCGC	2 cut(s)	336, 1228
AspS9I	GGNCC	9 cut(s)	39, 140, 222, 365, 737, 905, 1880, 2225, 2357
AsuC2I	CCSGG	5 cut(s)	86, 144, 206, 303, 1546
AsuHPI	GGTGA	5 cut(s)	262, 539, 1121, 1158, 2296
AsuII	TTCGAA	1 cut(s)	240
AvaII	GGWCC	7 cut(s)	39, 140, 222, 365, 905, 2225, 2357
BaeI	ACNNNNGTAYC	2 cut(s)	603, 636
BanII	GRGCYC	2 cut(s)	303, 1717
BarI	GAAGNNNNNNTAC	2 cut(s)	1897, 1929
BbsI	GAAGAC	1 cut(s)	489
Bbv12I	GWGCWC	2 cut(s)	318, 1717
BbvI	GCAGC	2 cut(s)	1262, 1645
BceAI	ACGGC	1 cut(s)	2200
BcgI	CGANNNNNNTGC	2 cut(s)	524, 558
BciT130I	CCWGG	3 cut(s)	625, 735, 1158
BciVI	GTATCC	1 cut(s)	1699
BcnI	CCSGG	5 cut(s)	86, 144, 206, 303, 1546
BcoDI	GTCTC	2 cut(s)	985, 1829
BfaI	CTAG	2 cut(s)	990, 1041
BfmI	CTRYAG	3 cut(s)	1026, 1287, 2016
BfrI	CTTAAG	1 cut(s)	2261
BfuAI	ACCTGC	1 cut(s)	639
BfuI	GTATCC	1 cut(s)	1699
BisI	GCNGC	6 cut(s)	18, 21, 307, 337, 1251, 1659
BlsI	GCNGC	6 cut(s)	19, 22, 308, 338, 1252, 1660
BmcAI	AGTACT	1 cut(s)	560
Bme1390I	CCNGG	8 cut(s)	86, 144, 206, 303, 625, 735, 1158, 1546
Bme18I	GGWCC	7 cut(s)	39, 140, 222, 365, 905, 2225, 2357
BmgT120I	GGNCC	9 cut(s)	39, 140, 222, 365, 737, 905, 1880, 2225, 2357
BmiI	GGNNCC	5 cut(s)	141, 366, 367, 629, 2226
BmrFI	CCNGG	8 cut(s)	86, 144, 206, 303, 625, 735, 1158, 1546
BmrI	ACTGGG	2 cut(s)	571, 1219
BmsI	GCATC	3 cut(s)	1066, 1225, 1387
BmuI	ACTGGG	2 cut(s)	571, 1219
BpiI	GAAGAC	1 cut(s)	489
BpmI	CTGGAG	2 cut(s)	1073, 1473
Bpu10I	CCTNAGC	1 cut(s)	2313
Bpu14I	TTCGAA	1 cut(s)	240
BpuEI	CTTGAG	2 cut(s)	1701, 1725
BpuMI	CCSGG	5 cut(s)	86, 144, 206, 303, 1546
BsaHI	GRCGYC	2 cut(s)	294, 355
BsaI	GGTCTC	1 cut(s)	985
BsaJI	CCNNGG	8 cut(s)	369, 623, 734, 1000, 1118, 1156, 1157, 1942
BsaWI	WCCGGW	2 cut(s)	115, 173
BsaXI	ACNNNNNCTCC	2 cut(s)	1065, 1095
Bsc4I	CCNNNNNNNGG	7 cut(s)	36, 48, 308, 374, 647, 841, 2222
Bse118I	RCCGGY	1 cut(s)	152
Bse1I	ACTGG	5 cut(s)	566, 1111, 1214, 1351, 1490
Bse3DI	GCAATG	1 cut(s)	1435
BseAI	TCCGGA	2 cut(s)	115, 173
BseBI	CCWGG	3 cut(s)	625, 735, 1158
BseDI	CCNNGG	8 cut(s)	369, 623, 734, 1000, 1118, 1156, 1157, 1942
BseGI	GGATG	8 cut(s)	57, 116, 174, 1012, 1426, 1496, 1933, 1952
BseLI	CCNNNNNNNGG	7 cut(s)	36, 48, 308, 374, 647, 841, 2222
BseMI	GCAATG	1 cut(s)	1435
BseMII	CTCAG	2 cut(s)	546, 2304
BseNI	ACTGG	5 cut(s)	566, 1111, 1214, 1351, 1490
BseRI	GAGGAG	1 cut(s)	873
BseXI	GCAGC	2 cut(s)	1262, 1645
BseYI	CCCAGC	1 cut(s)	826
Bsh1236I	CGCG	2 cut(s)	336, 371
BshFI	GGCC	6 cut(s)	8, 739, 1123, 1620, 1882, 2215
BsiHKAI	GWGCWC	2 cut(s)	318, 1717
BsiSI	CCGG	8 cut(s)	85, 116, 143, 153, 174, 206, 303, 1545
BslFI	GGGAC	6 cut(s)	13, 188, 378, 1789, 1878, 2238
BslI	CCNNNNNNNGG	7 cut(s)	36, 48, 308, 374, 647, 841, 2222
BsmAI	GTCTC	2 cut(s)	985, 1829
BsmFI	GGGAC	6 cut(s)	13, 188, 378, 1789, 1878, 2238
BsmI	GAATGC	2 cut(s)	1228, 2093
BsnI	GGCC	6 cut(s)	8, 739, 1123, 1620, 1882, 2215
Bso31I	GGTCTC	1 cut(s)	985
Bsp119I	TTCGAA	1 cut(s)	240
Bsp1286I	GDGCHC	3 cut(s)	303, 318, 1717
Bsp13I	TCCGGA	2 cut(s)	115, 173
Bsp143I	GATC	7 cut(s)	72, 280, 516, 815, 1375, 1523, 2335
BspANI	GGCC	6 cut(s)	8, 739, 1123, 1620, 1882, 2215
BspCNI	CTCAG	2 cut(s)	547, 2305
BspEI	TCCGGA	2 cut(s)	115, 173
BspFNI	CGCG	2 cut(s)	336, 371
BspLI	GGNNCC	5 cut(s)	141, 366, 367, 629, 2226
BspMAI	CTGCAG	1 cut(s)	1291
BspMI	ACCTGC	1 cut(s)	639
BspPI	GGATC	3 cut(s)	67, 288, 1531
BspT104I	TTCGAA	1 cut(s)	240
BspTI	CTTAAG	1 cut(s)	2261
BspTNI	GGTCTC	1 cut(s)	985
BsrBI	CCGCTC	2 cut(s)	23, 351
BsrDI	GCAATG	1 cut(s)	1435
BsrFI	RCCGGY	1 cut(s)	152
BsrI	ACTGG	5 cut(s)	566, 1111, 1214, 1351, 1490
BssAI	RCCGGY	1 cut(s)	152
BssECI	CCNNGG	8 cut(s)	369, 623, 734, 1000, 1118, 1156, 1157, 1942
BssMI	GATC	7 cut(s)	72, 280, 516, 815, 1375, 1523, 2335
BssNAI	GTATAC	1 cut(s)	1023
BssNI	GRCGYC	2 cut(s)	294, 355
BssT1I	CCWWGG	2 cut(s)	1000, 1118
Bst1107I	GTATAC	1 cut(s)	1023
Bst2UI	CCWGG	3 cut(s)	625, 735, 1158
Bst4CI	ACNGT	4 cut(s)	35, 1472, 1803, 2232
Bst6I	CTCTTC	1 cut(s)	891
BstACI	GRCGYC	2 cut(s)	294, 355
BstAFI	CTTAAG	1 cut(s)	2261
BstAPI	GCANNNNNTGC	1 cut(s)	2099
BstBI	TTCGAA	1 cut(s)	240
BstC8I	GCNNGC	2 cut(s)	353, 801
BstDEI	CTNAG	5 cut(s)	555, 728, 812, 1448, 2313
BstDSI	CCRYGG	2 cut(s)	369, 1942
BstF5I	GGATG	8 cut(s)	57, 116, 174, 1012, 1426, 1496, 1933, 1952
BstFNI	CGCG	2 cut(s)	336, 371
BstHHI	GCGC	2 cut(s)	336, 1228
BstKTI	GATC	7 cut(s)	75, 283, 519, 818, 1378, 1526, 2338
BstMAI	GTCTC	2 cut(s)	985, 1829
BstMBI	GATC	7 cut(s)	72, 280, 516, 815, 1375, 1523, 2335
BstMWI	GCNNNNNNNGC	4 cut(s)	14, 128, 342, 2099
BstNI	CCWGG	3 cut(s)	625, 735, 1158
BstNSI	RCATGY	1 cut(s)	1372
BstSCI	CCNGG	8 cut(s)	84, 142, 204, 301, 623, 733, 1156, 1544
BstSFI	CTRYAG	3 cut(s)	1026, 1287, 2016
BstUI	CGCG	2 cut(s)	336, 371
BstV1I	GCAGC	2 cut(s)	1262, 1645
BstV2I	GAAGAC	1 cut(s)	489
BstX2I	RGATCY	1 cut(s)	1523
BstXI	CCANNNNNNTGG	3 cut(s)	521, 710, 1628
BstYI	RGATCY	1 cut(s)	1523
BstZ17I	GTATAC	1 cut(s)	1023
BsuI	GTATCC	1 cut(s)	1699
BsuRI	GGCC	6 cut(s)	8, 739, 1123, 1620, 1882, 2215
BtgI	CCRYGG	2 cut(s)	369, 1942
BtgZI	GCGATG	1 cut(s)	354
BtsCI	GGATG	8 cut(s)	57, 116, 174, 1012, 1426, 1496, 1933, 1952
BtsI	GCAGTG	1 cut(s)	1296
BtsIMutI	CAGTG	5 cut(s)	1207, 1296, 1344, 1799, 2237
BveI	ACCTGC	1 cut(s)	639
Cac8I	GCNNGC	2 cut(s)	353, 801
CaiI	CAGNNNCTG	2 cut(s)	492, 2024
CfoI	GCGC	2 cut(s)	336, 1228
Cfr10I	RCCGGY	1 cut(s)	152
Cfr13I	GGNCC	9 cut(s)	39, 140, 222, 365, 737, 905, 1880, 2225, 2357
Cfr42I	CCGCGG	1 cut(s)	372
CseI	GACGC	2 cut(s)	283, 344
Csp6I	GTAC	6 cut(s)	559, 620, 651, 1773, 1957, 2324
CviQI	GTAC	6 cut(s)	559, 620, 651, 1773, 1957, 2324
DdeI	CTNAG	5 cut(s)	555, 728, 812, 1448, 2313
DpnI	GATC	7 cut(s)	74, 282, 518, 817, 1377, 1525, 2337
DpnII	GATC	7 cut(s)	72, 280, 516, 815, 1375, 1523, 2335
DraI	TTTAAA	1 cut(s)	1872
EaeI	YGGCCR	1 cut(s)	2213
Eam1104I	CTCTTC	1 cut(s)	891
EarI	CTCTTC	1 cut(s)	891
EciI	GGCGGA	1 cut(s)	324
Ecl136II	GAGCTC	1 cut(s)	1715
Eco130I	CCWWGG	2 cut(s)	1000, 1118
Eco24I	GRGCYC	2 cut(s)	303, 1717
Eco31I	GGTCTC	1 cut(s)	985
Eco47I	GGWCC	7 cut(s)	39, 140, 222, 365, 905, 2225, 2357
Eco53kI	GAGCTC	1 cut(s)	1715
EcoICRI	GAGCTC	1 cut(s)	1715
EcoO109I	RGGNCCY	1 cut(s)	365
EcoRI	GAATTC	1 cut(s)	1935
EcoRII	CCWGG	3 cut(s)	623, 733, 1156
EcoT14I	CCWWGG	2 cut(s)	1000, 1118
EcoT38I	GRGCYC	2 cut(s)	303, 1717
ErhI	CCWWGG	2 cut(s)	1000, 1118
FalI	AAGNNNNNCTT	2 cut(s)	951, 983
FaqI	GGGAC	6 cut(s)	13, 188, 378, 1789, 1878, 2238
FauI	CCCGC	3 cut(s)	260, 344, 376
FauNDI	CATATG	1 cut(s)	946
FblI	GTMKAC	1 cut(s)	1022
Fnu4HI	GCNGC	6 cut(s)	18, 21, 307, 337, 1251, 1659
FokI	GGATG	8 cut(s)	64, 123, 181, 1019, 1433, 1483, 1940, 1959
FriOI	GRGCYC	2 cut(s)	303, 1717
Fsp4HI	GCNGC	6 cut(s)	18, 21, 307, 337, 1251, 1659
FspBI	CTAG	2 cut(s)	990, 1041
FspI	TGCGCA	1 cut(s)	1227
GlaI	GCGC	2 cut(s)	335, 1227
GluI	GCNGC	6 cut(s)	18, 21, 307, 337, 1251, 1659
GsaI	CCCAGC	1 cut(s)	830
GsuI	CTGGAG	2 cut(s)	1073, 1473
HaeIII	GGCC	6 cut(s)	8, 739, 1123, 1620, 1882, 2215
HapII	CCGG	8 cut(s)	85, 116, 143, 153, 174, 206, 303, 1545
HgaI	GACGC	2 cut(s)	283, 344
HhaI	GCGC	2 cut(s)	336, 1228
Hin1I	GRCGYC	2 cut(s)	294, 355
Hin6I	GCGC	2 cut(s)	334, 1226
HinP1I	GCGC	2 cut(s)	334, 1226
HincII	GTYRAC	3 cut(s)	248, 1096, 2043
HindII	GTYRAC	3 cut(s)	248, 1096, 2043
HindIII	AAGCTT	2 cut(s)	707, 960
HpaII	CCGG	8 cut(s)	85, 116, 143, 153, 174, 206, 303, 1545
HphI	GGTGA	5 cut(s)	262, 539, 1121, 1158, 2296
Hpy166II	GTNNAC	5 cut(s)	248, 1023, 1096, 2043, 2326
Hpy8I	GTNNAC	5 cut(s)	248, 1023, 1096, 2043, 2326
Hpy99I	CGWCG	8 cut(s)	39, 110, 168, 233, 278, 288, 299, 360
HpyAV	CCTTC	8 cut(s)	236, 247, 786, 845, 1447, 1746, 1817, 1894
HpyCH4III	ACNGT	4 cut(s)	35, 1472, 1803, 2232
HpyCH4IV	ACGT	3 cut(s)	1767, 1867, 2144
HpyF10VI	GCNNNNNNNGC	4 cut(s)	14, 128, 342, 2099
HpyF3I	CTNAG	5 cut(s)	555, 728, 812, 1448, 2313
HpySE526I	ACGT	3 cut(s)	1767, 1867, 2144
Hsp92I	GRCGYC	2 cut(s)	294, 355
HspAI	GCGC	2 cut(s)	334, 1226
KflI	GGGWCCC	1 cut(s)	365
Kpn2I	TCCGGA	2 cut(s)	115, 173
KspI	CCGCGG	1 cut(s)	372
Kzo9I	GATC	7 cut(s)	72, 280, 516, 815, 1375, 1523, 2335
LmnI	GCTCC	6 cut(s)	28, 185, 193, 886, 1337, 2257
Lsp1109I	GCAGC	2 cut(s)	1262, 1645
LweI	GCATC	3 cut(s)	1066, 1225, 1387
MaeI	CTAG	2 cut(s)	990, 1041
MaeII	ACGT	3 cut(s)	1767, 1867, 2144
MaeIII	GTNAC	7 cut(s)	527, 783, 953, 1134, 1645, 2179, 2295
MalI	GATC	7 cut(s)	74, 282, 518, 817, 1377, 1525, 2337
MbiI	CCGCTC	2 cut(s)	23, 351
MboI	GATC	7 cut(s)	72, 280, 516, 815, 1375, 1523, 2335
MfeI	CAATTG	1 cut(s)	839
MflI	RGATCY	1 cut(s)	1523
MhlI	GDGCHC	3 cut(s)	303, 318, 1717
MlyI	GAGTC	3 cut(s)	457, 1367, 1619
MmeI	TCCRAC	3 cut(s)	17, 88, 146
MroI	TCCGGA	2 cut(s)	115, 173
MroXI	GAANNNNTTC	2 cut(s)	1282, 2110
MseI	TTAA	7 cut(s)	599, 1259, 1871, 2057, 2187, 2219, 2262
MslI	CAYNNNNRTG	3 cut(s)	745, 2036, 2282
MspA1I	CMGCKG	1 cut(s)	371
MspCI	CTTAAG	1 cut(s)	2261
MspI	CCGG	8 cut(s)	85, 116, 143, 153, 174, 206, 303, 1545
MspR9I	CCNGG	8 cut(s)	86, 144, 206, 303, 625, 735, 1158, 1546
MunI	CAATTG	1 cut(s)	839
Mva1269I	GAATGC	2 cut(s)	1228, 2093
MvaI	CCWGG	3 cut(s)	625, 735, 1158
MvnI	CGCG	2 cut(s)	336, 371
MwoI	GCNNNNNNNGC	4 cut(s)	14, 128, 342, 2099
NciI	CCSGG	5 cut(s)	86, 144, 206, 303, 1546
NdeI	CATATG	1 cut(s)	946
NdeII	GATC	7 cut(s)	72, 280, 516, 815, 1375, 1523, 2335
NlaIV	GGNNCC	5 cut(s)	141, 366, 367, 629, 2226
NmeAIII	GCCGAG	1 cut(s)	286
NmuCI	GTSAC	3 cut(s)	527, 953, 2179
NsbI	TGCGCA	1 cut(s)	1227
NspI	RCATGY	1 cut(s)	1372
NspV	TTCGAA	1 cut(s)	240
PasI	CCCWGGG	1 cut(s)	1157
PcsI	WCGNNNNNNNCGW	1 cut(s)	237
PctI	GAATGC	2 cut(s)	1228, 2093
PdmI	GAANNNNTTC	2 cut(s)	1282, 2110
PflMI	CCANNNNNTGG	1 cut(s)	647
PfoI	TCCNGGA	1 cut(s)	204
PkrI	GCNGC	6 cut(s)	19, 22, 308, 338, 1252, 1660
PleI	GAGTC	3 cut(s)	456, 1366, 1618
PpsI	GAGTC	3 cut(s)	456, 1366, 1618
PpuMI	RGGWCCY	1 cut(s)	365
PsiI	TTATAA	2 cut(s)	914, 1332
Psp124BI	GAGCTC	1 cut(s)	1717
Psp5II	RGGWCCY	1 cut(s)	365
Psp6I	CCWGG	3 cut(s)	623, 733, 1156
PspFI	CCCAGC	1 cut(s)	826
PspGI	CCWGG	3 cut(s)	623, 733, 1156
PspN4I	GGNNCC	5 cut(s)	141, 366, 367, 629, 2226
PspPI	GGNCC	9 cut(s)	39, 140, 222, 365, 737, 905, 1880, 2225, 2357
PspPPI	RGGWCCY	1 cut(s)	365
PstI	CTGCAG	1 cut(s)	1291
PstNI	CAGNNNCTG	2 cut(s)	492, 2024
PsuI	RGATCY	1 cut(s)	1523
RsaI	GTAC	6 cut(s)	560, 621, 652, 1774, 1958, 2325
RsaNI	GTAC	6 cut(s)	559, 620, 651, 1773, 1957, 2324
RseI	CAYNNNNRTG	3 cut(s)	745, 2036, 2282
SacI	GAGCTC	1 cut(s)	1717
SacII	CCGCGG	1 cut(s)	372
SaqAI	TTAA	7 cut(s)	599, 1259, 1871, 2057, 2187, 2219, 2262
SatI	GCNGC	6 cut(s)	18, 21, 307, 337, 1251, 1659
Sau3AI	GATC	7 cut(s)	72, 280, 516, 815, 1375, 1523, 2335
Sau96I	GGNCC	9 cut(s)	39, 140, 222, 365, 737, 905, 1880, 2225, 2357
ScaI	AGTACT	1 cut(s)	560
SchI	GAGTC	3 cut(s)	457, 1367, 1619
ScrFI	CCNGG	8 cut(s)	86, 144, 206, 303, 625, 735, 1158, 1546
SduI	GDGCHC	3 cut(s)	303, 318, 1717
SfaNI	GCATC	3 cut(s)	1066, 1225, 1387
SfcI	CTRYAG	3 cut(s)	1026, 1287, 2016
Sfr303I	CCGCGG	1 cut(s)	372
SfuI	TTCGAA	1 cut(s)	240
SgrBI	CCGCGG	1 cut(s)	372
SinI	GGWCC	7 cut(s)	39, 140, 222, 365, 905, 2225, 2357
SmiMI	CAYNNNNRTG	3 cut(s)	745, 2036, 2282
SmlI	CTYRAG	3 cut(s)	1716, 1740, 2261
SmoI	CTYRAG	3 cut(s)	1716, 1740, 2261
SspMI	CTAG	2 cut(s)	990, 1041
SstI	GAGCTC	1 cut(s)	1717
StyD4I	CCNGG	8 cut(s)	84, 142, 204, 301, 623, 733, 1156, 1544
StyI	CCWWGG	2 cut(s)	1000, 1118
TaaI	ACNGT	4 cut(s)	35, 1472, 1803, 2232
TaiI	ACGT	3 cut(s)	1770, 1870, 2147
TaqI	TCGA	8 cut(s)	231, 240, 297, 446, 846, 1790, 2334, 2369
TaqII	GACCGA	1 cut(s)	210
TatI	WGTACW	2 cut(s)	558, 1956
TauI	GCSGC	4 cut(s)	20, 23, 309, 339
Tru1I	TTAA	7 cut(s)	599, 1259, 1871, 2057, 2187, 2219, 2262
Tru9I	TTAA	7 cut(s)	599, 1259, 1871, 2057, 2187, 2219, 2262
TscAI	CASTG	5 cut(s)	1214, 1296, 1351, 1806, 2237
TseFI	GTSAC	3 cut(s)	527, 953, 2179
TseI	GCWGC	2 cut(s)	1250, 1658
Tsp45I	GTSAC	3 cut(s)	527, 953, 2179
TspGWI	ACGGA	3 cut(s)	15, 1536, 1931
TspRI	CASTG	5 cut(s)	1214, 1296, 1351, 1806, 2237
Van91I	CCANNNNNTGG	1 cut(s)	647
Vha464I	CTTAAG	1 cut(s)	2261
VpaK11BI	GGWCC	7 cut(s)	39, 140, 222, 365, 905, 2225, 2357
XapI	RAATTY	6 cut(s)	474, 658, 1266, 1436, 1549, 1935
XceI	RCATGY	1 cut(s)	1372
XmiI	GTMKAC	1 cut(s)	1022
XmnI	GAANNNNTTC	2 cut(s)	1282, 2110
XspI	CTAG	2 cut(s)	990, 1041
ZrmI	AGTACT	1 cut(s)	560

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.