Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014584

Physical Location & Seq

Genomic Coordinates Reverse (-)

28947743 .. 28951655

Gene Structure 3913 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G77030.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2538 bp

ATGGTTGAAGGAAAGGGCTTCCTCGTGAGCTCAGTGACTGAGCTTCACCGTAAAGAGAAACAGAAGAAGAAAGGCAAATCTGGAGGATTCGAGTCACTAAATCTCGGACCTAATGTCTTCAATGCAATCAAGAAAAAGGGTTACAAAGTTCCTACACCAATTCAGAGGAAGACGATGCCGCTTATTCTCTCTGGCGTCGATGTTGTCGCTATGGCTCGAACCGGTTCTGGAAAAACCGCAGCTTTTCTCATCCCTATGCTTGAGAAGCTCAAACAACACGTTCCTCAAGGAGGTGTTAGAGCACTCATCTTGTCTCCTACTCGTGACTTAGCTGAGCAGACTCTGAAGTTCACCAAGGAACTTGGCAAATTTACAGATCTTCGTGTATCCTTACTGGTTGGTGGAGATAGTATGGAAGACCAGTTTGAAGAGTTAACAAAGGGACCTGATGTGATTATTGCTACTCCTGGGAGACTTATGCATCTTTTATCTGAGGTTGATGATATGACGCTAAGGACGGTTGAGTATGTAGTTTTTGATGAGGCAGATAGTCTGTTTGGTATGGGGTTTGCTGAACAGTTACATCAGATTCTAACTCAGTTAAGTGAGAATAGGCAGACTTTGCTATTTAGTGCCACTTTACCAAGTGCCCTTGCTGAATTTGCAAAGGCTGGTTTGCGTGAGCCTCAGCTTGTTCGTTTAGATGTTGAGAATAAGATAAGTCCCGATTTGAAGCTTTCGTTTTTGACTGTACGACCGGAAGAGAAGTATTCTGCATTGCTGTACTTGGTTAGGGAGCATATAAGTTCAGATCAACAGACCTTGATATTTGTTTCGACAAAGCATCATGTGGAATTTGTCAACTCATTGTTTAAGTTGGAGAATATTGAGCCATCTGTATGTTATGGTGATATGGATCAAGATGCACGTAAGATTCATGTATCAAGGTTCAGAGCGAGGAAGACTATGTTGTTGATTGTGACAGATATTGCTGCTAGGGGTATTGACATACCATTGCTTGATAATGTTATAAACTGGGATTTCCCTCCGAGACCGAAGATTTTTGTTCATCGTGTGGGAAGAGCTGCCAGGGCTGGTCGTACAGGCTGTGCATATTCCTTTGTTACACCTGAGGATATGCCATATATGTTGGATCTTCATCTGTTCCTCTCAAAACCGGTCAGGCCAGCGCCCACTGAGGATGAGGTCTTGAAGAACATGGAAGAAGTAATGACCAAAACTAGTCAAGCAATTGATAGTGGAGTCACCGTCTATGGTCGTTTCCCACAGAAAACCATTGATCTTATCTTTAATAGAACCAGAGAAATGATTGATTCATCTGCAGAATTAGATTCCCTTGAACGAACATCCACCAAAGCTTTTCGCTTGTATTCAAAAACAAAACCCTCACCGTCAAAAGAATCCATTAGGAGAGCAAAGGATTTACCACGAGAAGGCTTGCATCCCATCTTCAGAAGCATTATTGAAACTGGGGAATTAGAGGCAATGTCGTTTTTCCAGAAAATTAAAAATTTCAGACCCAAGCAAACGATACTTGAAGCAGAAGGTGAAGTTGCCAAATCCAAACATGTGAAGGGTCCCGCTGGCCAATGGGTTGATGTGATGAAGAAGAAAAGAGCTATTCATGAGGAGATCATCAATACAAGACATCAGCAGAATCAAAAGACTTCCAATAATCATTTGGAGATGGAAGCTGAACCTACAACTTCTTTTGTTGATGGCACAGTAGAGGGAAGCAAAGTAAGCGGTAAGAAAAGAAAGGCACAAGAAACCTTCAAGGACGATGAATTCTTTATCAGCTCCATACCAGTAAATCATCATTCAGAGGCAGGGCTATCTCTGAGGGGTAACGAAGGATTTGGATCGAATAGGTTGGACGCTGCTGTTCTAGATCTAGTTGCAGATGATGGGCAAGGGATAAAGCAGCAACAATCAAATTATCACTGGGACAAGAAAGGTAAGAAGTACATCAAGTTGAACAATGGAGATCGAGTAACAGCCAGTGGAAAGATAAAGACAGAGAGTGGAGCTAAAGCGACTGCAAAGAAGACAGGGATATACAAGAGATGGCAAGAGAGGTCTCACAAGAAAGTATCCCGAGACAGCGGTGATGCAGACGAAACCACACGAATGTCAGGTAGAGGAGGCCGAGATGGGAAGAGACGGCAAGGATCAGTGCCTAACGCACATGTTCGTTCAGAGATCAAAGATCTGGACCAGGTGCGTAAGGAGAGACAGCAAAAGGCAAACAAAGTTTCTTATCTGCAGAGCAAGAGAGGCGGAAGAGGTGGAAGAGGTGGGGCCAGGGGAGGCCGTGGTGGTGGGGCCAGGGGAGGCCGTGGTGGCAGCAGGGATTTTGGTGGTGGAGGAAGGGATTTTGGCAGTAGCAGCGATAGAGGTGGAAGAAGTGGCGGTAGGGATTTTGGTGGTCGTCGTGGTGGTGCTAGCACTAGTAGCAGAGGTGGTAAGAGAGGAGGAGGAAGAGGAGGTGGAGGTAAACGTGGGAGAGGAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DBP10CT DEAD Helicase_C

Protein Analysis

845

Amino Acids

93.5

Weight (kDa)

9.9

Isoelectric Point (pI)

40.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	52 - 220	4.2e-51	DEAD/DEAH box helicase
ResIII	PF04851	71 - 214	5.8e-07	Type III restriction enzyme, res subunit
Helicase_C	PF00271	256 - 366	5.9e-24	Helicase conserved C-terminal domain
DBP10CT	PF08147	638 - 699	9.3e-14	DBP10CT (NUC160) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014584)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77030
fragaria_vesca	FvH4_2g15160 FvH4_2g15160
malus_domestica	MD07G1108300.v1.1
prunus_persica	Prupe.2G131100_v2.0.a1 Prupe.2G131100_v2.0.a1
pyrus_communis	pycom07g09270
rosa_chinensis	RchiOBHm_Chr6g0277821
rosa_laevigata	RLG00000013266
rosa_multiflora	Rmu_sc0001874.1_g000004
rosa_roxburghii	Rroxscaffold_7G00190870
rosa_rugosa	Rorug06G0111700
rosa_samantha	Rh6AG222500 Rh6BG226000 Rh6CG228500 Rh6DG219600
rosa_wichuraiana	Rw6G019430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1031
AciI	CCGC	7 cut(s)	179, 237, 1602, 1767, 2127, 2301, 2433
AclWI	GGATC	4 cut(s)	924, 1161, 1891, 2200
AcoI	YGGCCR	1 cut(s)	1606
AcsI	RAATTY	5 cut(s)	368, 659, 854, 1531, 1808
AcuI	CTGAAG	2 cut(s)	365, 1456
AcyI	GRCGYC	1 cut(s)	195
AfaI	GTAC	4 cut(s)	753, 785, 1102, 1988
AfiI	CCNNNNNNNGG	2 cut(s)	290, 1454
AflIII	ACRYGT	3 cut(s)	277, 1588, 2209
AgeI	ACCGGT	2 cut(s)	221, 1177
AhdI	GACNNNNNGTC	1 cut(s)	113
AhlI	ACTAGT	2 cut(s)	1241, 2471
AjnI	CCWGG	5 cut(s)	466, 1088, 2238, 2324, 2348
Alw21I	GWGCWC	2 cut(s)	32, 304
Alw26I	GTCTC	7 cut(s)	318, 466, 1045, 2106, 2115, 2176, 2248
AlwI	GGATC	4 cut(s)	924, 1161, 1891, 2200
AlwNI	CAGNNNCTG	2 cut(s)	38, 343
Ama87I	CYCGRG	1 cut(s)	2118
AoxI	GGCC	7 cut(s)	1184, 1606, 2167, 2322, 2332, 2346, 2356
ApeKI	GCWGC	7 cut(s)	239, 992, 1085, 1901, 1945, 2367, 2409
ApoI	RAATTY	5 cut(s)	368, 659, 854, 1531, 1808
AsiGI	ACCGGT	2 cut(s)	221, 1177
Asp700I	GAANNNNTTC	1 cut(s)	223
AspLEI	GCGC	1 cut(s)	1192
AspS9I	GGNCC	6 cut(s)	107, 443, 1598, 2236, 2322, 2346
AsuHPI	GGTGA	7 cut(s)	38, 343, 920, 1258, 1401, 1580, 2141
AsuNHI	GCTAGC	1 cut(s)	2465
AvaI	CYCGRG	1 cut(s)	2118
AvaII	GGWCC	4 cut(s)	107, 443, 1598, 2236
AxyI	CCTNAGG	1 cut(s)	1131
BaeGI	GKGCMC	1 cut(s)	652
BalI	TGGCCA	1 cut(s)	1608
BanII	GRGCYC	1 cut(s)	32
BauI	CACGAG	3 cut(s)	23, 321, 1449
BbsI	GAAGAC	5 cut(s)	109, 176, 423, 968, 2075
Bbv12I	GWGCWC	2 cut(s)	32, 304
BbvCI	CCTCAGC	1 cut(s)	687
BbvI	GCAGC	7 cut(s)	251, 979, 1072, 1888, 1957, 2379, 2421
BccI	CCATC	7 cut(s)	901, 1475, 1702, 1733, 1922, 2082, 2168
BceAI	ACGGC	3 cut(s)	2201, 2319, 2343
BciT130I	CCWGG	5 cut(s)	468, 1090, 2240, 2326, 2350
BciVI	GTATCC	2 cut(s)	397, 2125
BcoDI	GTCTC	7 cut(s)	318, 466, 1045, 2106, 2115, 2176, 2248
BcuI	ACTAGT	2 cut(s)	1241, 2471
BfaI	CTAG	6 cut(s)	996, 1242, 1910, 1916, 2466, 2472
BfmI	CTRYAG	2 cut(s)	1341, 2285
BfoI	RGCGCY	1 cut(s)	1193
BfuI	GTATCC	2 cut(s)	397, 2125
BglI	GCCNNNNNGGC	1 cut(s)	2364
BglII	AGATCT	3 cut(s)	376, 1912, 2230
BisI	GCNGC	8 cut(s)	179, 240, 993, 1086, 1902, 1946, 2368, 2410
BlpI	GCTNAGC	1 cut(s)	333
BlsI	GCNGC	8 cut(s)	180, 241, 994, 1087, 1903, 1947, 2369, 2411
Bme1390I	CCNGG	5 cut(s)	468, 1090, 2240, 2326, 2350
Bme18I	GGWCC	4 cut(s)	107, 443, 1598, 2236
BmeRI	GACNNNNNGTC	1 cut(s)	113
BmeT110I	CYCGRG	1 cut(s)	2118
BmgT120I	GGNCC	6 cut(s)	107, 443, 1598, 2236, 2322, 2346
BmiI	GGNNCC	5 cut(s)	444, 1599, 1600, 2323, 2347
BmrFI	CCNGG	5 cut(s)	468, 1090, 2240, 2326, 2350
BmrI	ACTGGG	3 cut(s)	1045, 1500, 1975
BmsI	GCATC	6 cut(s)	165, 490, 853, 913, 1471, 2122
BmtI	GCTAGC	1 cut(s)	2469
BmuI	ACTGGG	3 cut(s)	1045, 1500, 1975
BpiI	GAAGAC	5 cut(s)	109, 176, 423, 968, 2075
BpmI	CTGGAG	1 cut(s)	102
Bpu10I	CCTNAGC	2 cut(s)	512, 687
Bpu1102I	GCTNAGC	1 cut(s)	333
BpuEI	CTTGAG	2 cut(s)	270, 281
BsaAI	YACGTR	1 cut(s)	929
BsaBI	GATNNNNATC	3 cut(s)	915, 1158, 1882
BsaHI	GRCGYC	1 cut(s)	195
BsaI	GGTCTC	2 cut(s)	1045, 2106
BsaJI	CCNNGG	7 cut(s)	354, 467, 1089, 2325, 2335, 2349, 2359
BsaWI	WCCGGW	3 cut(s)	221, 757, 1177
BsaXI	ACNNNNNCTCC	4 cut(s)	1254, 1284, 1998, 2028
Bsc4I	CCNNNNNNNGG	2 cut(s)	290, 1454
Bse118I	RCCGGY	2 cut(s)	221, 1177
Bse1I	ACTGG	7 cut(s)	399, 421, 1040, 1495, 1829, 1970, 2022
Bse21I	CCTNAGG	1 cut(s)	1131
Bse3DI	GCAATG	3 cut(s)	776, 1013, 1512
Bse8I	GATNNNNATC	3 cut(s)	915, 1158, 1882
BseBI	CCWGG	5 cut(s)	468, 1090, 2240, 2326, 2350
BseDI	CCNNGG	7 cut(s)	354, 467, 1089, 2325, 2335, 2349, 2359
BseGI	GGATG	4 cut(s)	249, 1207, 1367, 1462
BseJI	GATNNNNATC	3 cut(s)	915, 1158, 1882
BseLI	CCNNNNNNNGG	2 cut(s)	290, 1454
BseMI	GCAATG	3 cut(s)	776, 1013, 1512
BseMII	CTCAG	9 cut(s)	30, 45, 324, 483, 611, 701, 1122, 1188, 1853
BseNI	ACTGG	7 cut(s)	399, 421, 1040, 1495, 1829, 1970, 2022
BseRI	GAGGAG	5 cut(s)	1664, 2178, 2508, 2511, 2520
BseSI	GKGCMC	1 cut(s)	652
BseXI	GCAGC	7 cut(s)	251, 979, 1072, 1888, 1957, 2379, 2421
Bsh1285I	CGRYCG	1 cut(s)	758
BshFI	GGCC	7 cut(s)	1186, 1608, 2169, 2324, 2334, 2348, 2358
BshTI	ACCGGT	2 cut(s)	221, 1177
BsiEI	CGRYCG	1 cut(s)	758
BsiHKAI	GWGCWC	2 cut(s)	32, 304
BsiHKCI	CYCGRG	1 cut(s)	2118
BsiSI	CCGG	3 cut(s)	222, 758, 1178
BslFI	GGGAC	4 cut(s)	456, 708, 1584, 1982
BslI	CCNNNNNNNGG	2 cut(s)	290, 1454
BsmAI	GTCTC	7 cut(s)	318, 466, 1045, 2106, 2115, 2176, 2248
BsmBI	CGTCTC	1 cut(s)	2176
BsmFI	GGGAC	4 cut(s)	456, 708, 1584, 1982
BsnI	GGCC	7 cut(s)	1186, 1608, 2169, 2324, 2334, 2348, 2358
Bso31I	GGTCTC	2 cut(s)	1045, 2106
BsoBI	CYCGRG	1 cut(s)	2118
Bsp1286I	GDGCHC	3 cut(s)	32, 304, 652
Bsp1720I	GCTNAGC	1 cut(s)	333
BspACI	CCGC	7 cut(s)	179, 237, 1602, 1767, 2127, 2301, 2433
BspANI	GGCC	7 cut(s)	1186, 1608, 2169, 2324, 2334, 2348, 2358
BspCNI	CTCAG	9 cut(s)	31, 44, 325, 484, 610, 700, 1123, 1189, 1854
BspHI	TCATGA	1 cut(s)	1645
BspLI	GGNNCC	5 cut(s)	444, 1599, 1600, 2323, 2347
BspMAI	CTGCAG	2 cut(s)	1345, 2289
BspOI	GCTAGC	1 cut(s)	2469
BspPI	GGATC	4 cut(s)	924, 1161, 1891, 2200
BspQI	GCTCTTC	1 cut(s)	1075
BspTNI	GGTCTC	2 cut(s)	1045, 2106
BsrDI	GCAATG	3 cut(s)	776, 1013, 1512
BsrFI	RCCGGY	2 cut(s)	221, 1177
BsrI	ACTGG	7 cut(s)	399, 421, 1040, 1495, 1829, 1970, 2022
BssAI	RCCGGY	2 cut(s)	221, 1177
BssECI	CCNNGG	7 cut(s)	354, 467, 1089, 2325, 2335, 2349, 2359
BssNI	GRCGYC	1 cut(s)	195
BssSI	CACGAG	3 cut(s)	23, 321, 1449
BssT1I	CCWWGG	1 cut(s)	354
Bst2BI	CACGAG	3 cut(s)	23, 321, 1449
Bst2UI	CCWGG	5 cut(s)	468, 1090, 2240, 2326, 2350
Bst4CI	ACNGT	7 cut(s)	50, 520, 579, 751, 1270, 1413, 1747
Bst6I	CTCTTC	7 cut(s)	423, 756, 1075, 2174, 2299, 2308, 2497
BstACI	GRCGYC	1 cut(s)	195
BstAPI	GCANNNNNTGC	1 cut(s)	622
BstBAI	YACGTR	1 cut(s)	929
BstC8I	GCNNGC	4 cut(s)	1188, 1460, 1606, 2467
BstDSI	CCRYGG	2 cut(s)	2335, 2359
BstENI	CCTNNNNNAGG	1 cut(s)	288
BstF5I	GGATG	4 cut(s)	249, 1207, 1367, 1462
BstH2I	RGCGCY	1 cut(s)	1193
BstHHI	GCGC	1 cut(s)	1192
BstMAI	GTCTC	7 cut(s)	318, 466, 1045, 2106, 2115, 2176, 2248
BstMCI	CGRYCG	1 cut(s)	758
BstMWI	GCNNNNNNNGC	9 cut(s)	265, 622, 662, 1091, 1764, 2298, 2364, 2409, 2475
BstNI	CCWGG	5 cut(s)	468, 1090, 2240, 2326, 2350
BstNSI	RCATGY	2 cut(s)	1592, 2213
BstSCI	CCNGG	5 cut(s)	466, 1088, 2238, 2324, 2348
BstSFI	CTRYAG	2 cut(s)	1341, 2285
BstSLI	GKGCMC	1 cut(s)	652
BstV1I	GCAGC	7 cut(s)	251, 979, 1072, 1888, 1957, 2379, 2421
BstV2I	GAAGAC	5 cut(s)	109, 176, 423, 968, 2075
BstX2I	RGATCY	4 cut(s)	376, 1153, 1912, 2230
BstYI	RGATCY	4 cut(s)	376, 1153, 1912, 2230
Bsu36I	CCTNAGG	1 cut(s)	1131
BsuI	GTATCC	2 cut(s)	397, 2125
BsuRI	GGCC	7 cut(s)	1186, 1608, 2169, 2324, 2334, 2348, 2358
BtgI	CCRYGG	2 cut(s)	2335, 2359
BtsCI	GGATG	4 cut(s)	249, 1207, 1367, 1462
BtsIMutI	CAGTG	5 cut(s)	39, 1194, 1963, 2029, 2202
Cac8I	GCNNGC	4 cut(s)	1188, 1460, 1606, 2467
CaiI	CAGNNNCTG	2 cut(s)	38, 343
CciI	TCATGA	1 cut(s)	1645
CfoI	GCGC	1 cut(s)	1192
Cfr10I	RCCGGY	2 cut(s)	221, 1177
Cfr13I	GGNCC	6 cut(s)	107, 443, 1598, 2236, 2322, 2346
CseI	GACGC	3 cut(s)	184, 517, 1907
CsiI	ACCWGGT	1 cut(s)	2238
Csp6I	GTAC	4 cut(s)	752, 784, 1101, 1987
CspAI	ACCGGT	2 cut(s)	221, 1177
CviAII	CATG	6 cut(s)	848, 938, 1219, 1589, 1646, 2210
CviQI	GTAC	4 cut(s)	752, 784, 1101, 1987
DriI	GACNNNNNGTC	1 cut(s)	113
EaeI	YGGCCR	1 cut(s)	1606
Eam1104I	CTCTTC	7 cut(s)	423, 756, 1075, 2174, 2299, 2308, 2497
Eam1105I	GACNNNNNGTC	1 cut(s)	113
EarI	CTCTTC	7 cut(s)	423, 756, 1075, 2174, 2299, 2308, 2497
EciI	GGCGGA	1 cut(s)	2316
Ecl136II	GAGCTC	1 cut(s)	30
Eco130I	CCWWGG	1 cut(s)	354
Eco24I	GRGCYC	1 cut(s)	32
Eco31I	GGTCTC	2 cut(s)	1045, 2106
Eco47I	GGWCC	4 cut(s)	107, 443, 1598, 2236
Eco53kI	GAGCTC	1 cut(s)	30
Eco57I	CTGAAG	2 cut(s)	365, 1456
Eco81I	CCTNAGG	1 cut(s)	1131
Eco88I	CYCGRG	1 cut(s)	2118
EcoICRI	GAGCTC	1 cut(s)	30
EcoNI	CCTNNNNNAGG	1 cut(s)	288
EcoO109I	RGGNCCY	2 cut(s)	443, 1598
EcoRI	GAATTC	1 cut(s)	1808
EcoRII	CCWGG	5 cut(s)	466, 1088, 2238, 2324, 2348
EcoT14I	CCWWGG	1 cut(s)	354
EcoT22I	ATGCAT	1 cut(s)	483
EcoT38I	GRGCYC	1 cut(s)	32
ErhI	CCWWGG	1 cut(s)	354
Esp3I	CGTCTC	1 cut(s)	2176
FaeI	CATG	6 cut(s)	851, 941, 1222, 1592, 1649, 2213
FaqI	GGGAC	4 cut(s)	456, 708, 1584, 1982
FatI	CATG	6 cut(s)	847, 937, 1218, 1588, 1645, 2209
FauI	CCCGC	1 cut(s)	1609
Fnu4HI	GCNGC	8 cut(s)	179, 240, 993, 1086, 1902, 1946, 2368, 2410
FokI	GGATG	4 cut(s)	236, 1214, 1354, 1449
FriOI	GRGCYC	1 cut(s)	32
Fsp4HI	GCNGC	8 cut(s)	179, 240, 993, 1086, 1902, 1946, 2368, 2410
FspBI	CTAG	6 cut(s)	996, 1242, 1910, 1916, 2466, 2472
GlaI	GCGC	1 cut(s)	1191
GluI	GCNGC	8 cut(s)	179, 240, 993, 1086, 1902, 1946, 2368, 2410
GsuI	CTGGAG	1 cut(s)	102
HaeII	RGCGCY	1 cut(s)	1193
HaeIII	GGCC	7 cut(s)	1186, 1608, 2169, 2324, 2334, 2348, 2358
HapII	CCGG	3 cut(s)	222, 758, 1178
HgaI	GACGC	3 cut(s)	184, 517, 1907
HhaI	GCGC	1 cut(s)	1192
Hin1I	GRCGYC	1 cut(s)	195
Hin1II	CATG	6 cut(s)	851, 941, 1222, 1592, 1649, 2213
Hin6I	GCGC	1 cut(s)	1190
HinP1I	GCGC	1 cut(s)	1190
HincII	GTYRAC	2 cut(s)	435, 862
HindII	GTYRAC	2 cut(s)	435, 862
HindIII	AAGCTT	2 cut(s)	734, 1377
HpaI	GTTAAC	1 cut(s)	435
HpaII	CCGG	3 cut(s)	222, 758, 1178
HphI	GGTGA	7 cut(s)	38, 343, 920, 1258, 1401, 1580, 2141
Hpy166II	GTNNAC	4 cut(s)	351, 435, 862, 2519
Hpy8I	GTNNAC	4 cut(s)	351, 435, 862, 2519
Hpy99I	CGWCG	2 cut(s)	200, 2457
HpyAV	CCTTC	6 cut(s)	1448, 1559, 1588, 1804, 1868, 2385
HpyCH4III	ACNGT	7 cut(s)	50, 520, 579, 751, 1270, 1413, 1747
HpyCH4IV	ACGT	3 cut(s)	279, 928, 2521
HpyF10VI	GCNNNNNNNGC	9 cut(s)	265, 622, 662, 1091, 1764, 2298, 2364, 2409, 2475
HpySE526I	ACGT	3 cut(s)	279, 928, 2521
Hsp92I	GRCGYC	1 cut(s)	195
Hsp92II	CATG	6 cut(s)	851, 941, 1222, 1592, 1649, 2213
HspAI	GCGC	1 cut(s)	1190
KflI	GGGWCCC	1 cut(s)	1598
KspAI	GTTAAC	1 cut(s)	435
LguI	GCTCTTC	1 cut(s)	1075
LmnI	GCTCC	3 cut(s)	796, 1826, 2048
Lsp1109I	GCAGC	7 cut(s)	251, 979, 1072, 1888, 1957, 2379, 2421
LweI	GCATC	6 cut(s)	165, 490, 853, 913, 1471, 2122
MabI	ACCWGGT	1 cut(s)	2238
MaeI	CTAG	6 cut(s)	996, 1242, 1910, 1916, 2466, 2472
MaeII	ACGT	3 cut(s)	279, 928, 2521
MfeI	CAATTG	1 cut(s)	1251
MflI	RGATCY	4 cut(s)	376, 1153, 1912, 2230
MhlI	GDGCHC	3 cut(s)	32, 304, 652
MlsI	TGGCCA	1 cut(s)	1608
MluNI	TGGCCA	1 cut(s)	1608
MlyI	GAGTC	3 cut(s)	101, 334, 1272
MmeI	TCCRAC	3 cut(s)	858, 1131, 1875
Mox20I	TGGCCA	1 cut(s)	1608
Mph1103I	ATGCAT	1 cut(s)	483
MroXI	GAANNNNTTC	1 cut(s)	223
MscI	TGGCCA	1 cut(s)	1608
MseI	TTAA	5 cut(s)	434, 602, 873, 1311, 1527
MslI	CAYNNNNRTG	3 cut(s)	254, 898, 2150
Msp20I	TGGCCA	1 cut(s)	1608
MspA1I	CMGCKG	2 cut(s)	1604, 2127
MspI	CCGG	3 cut(s)	222, 758, 1178
MspR9I	CCNGG	5 cut(s)	468, 1090, 2240, 2326, 2350
MunI	CAATTG	1 cut(s)	1251
MvaI	CCWGG	5 cut(s)	468, 1090, 2240, 2326, 2350
MwoI	GCNNNNNNNGC	9 cut(s)	265, 622, 662, 1091, 1764, 2298, 2364, 2409, 2475
NheI	GCTAGC	1 cut(s)	2465
NlaIII	CATG	6 cut(s)	851, 941, 1222, 1592, 1649, 2213
NlaIV	GGNNCC	5 cut(s)	444, 1599, 1600, 2323, 2347
NmeAIII	GCCGAG	1 cut(s)	2195
NmuCI	GTSAC	5 cut(s)	34, 93, 323, 979, 1264
NsiI	ATGCAT	1 cut(s)	483
NspI	RCATGY	2 cut(s)	1592, 2213
PagI	TCATGA	1 cut(s)	1645
PciI	ACATGT	2 cut(s)	1588, 2209
PciSI	GCTCTTC	1 cut(s)	1075
PdmI	GAANNNNTTC	1 cut(s)	223
PfeI	GAWTC	7 cut(s)	87, 589, 934, 1334, 1352, 1421, 1678
PinAI	ACCGGT	2 cut(s)	221, 1177
PkrI	GCNGC	8 cut(s)	180, 241, 994, 1087, 1903, 1947, 2369, 2411
PleI	GAGTC	3 cut(s)	100, 334, 1271
PpsI	GAGTC	3 cut(s)	100, 334, 1271
Ppu21I	YACGTR	1 cut(s)	929
PpuMI	RGGWCCY	2 cut(s)	443, 1598
PscI	ACATGT	2 cut(s)	1588, 2209
PsiI	TTATAA	1 cut(s)	1031
Psp124BI	GAGCTC	1 cut(s)	32
Psp5II	RGGWCCY	2 cut(s)	443, 1598
Psp6I	CCWGG	5 cut(s)	466, 1088, 2238, 2324, 2348
PspGI	CCWGG	5 cut(s)	466, 1088, 2238, 2324, 2348
PspN4I	GGNNCC	5 cut(s)	444, 1599, 1600, 2323, 2347
PspPI	GGNCC	6 cut(s)	107, 443, 1598, 2236, 2322, 2346
PspPPI	RGGWCCY	2 cut(s)	443, 1598
PstI	CTGCAG	2 cut(s)	1345, 2289
PstNI	CAGNNNCTG	2 cut(s)	38, 343
PsuI	RGATCY	4 cut(s)	376, 1153, 1912, 2230
RsaI	GTAC	4 cut(s)	753, 785, 1102, 1988
RsaNI	GTAC	4 cut(s)	752, 784, 1101, 1987
RseI	CAYNNNNRTG	3 cut(s)	254, 898, 2150
SacI	GAGCTC	1 cut(s)	32
SapI	GCTCTTC	1 cut(s)	1075
SaqAI	TTAA	5 cut(s)	434, 602, 873, 1311, 1527
SatI	GCNGC	8 cut(s)	179, 240, 993, 1086, 1902, 1946, 2368, 2410
Sau96I	GGNCC	6 cut(s)	107, 443, 1598, 2236, 2322, 2346
SchI	GAGTC	3 cut(s)	101, 334, 1272
ScrFI	CCNGG	5 cut(s)	468, 1090, 2240, 2326, 2350
SduI	GDGCHC	3 cut(s)	32, 304, 652
SexAI	ACCWGGT	1 cut(s)	2238
SfaNI	GCATC	6 cut(s)	165, 490, 853, 913, 1471, 2122
SfcI	CTRYAG	2 cut(s)	1341, 2285
SinI	GGWCC	4 cut(s)	107, 443, 1598, 2236
SmiMI	CAYNNNNRTG	3 cut(s)	254, 898, 2150
SmlI	CTYRAG	2 cut(s)	260, 285
SmoI	CTYRAG	2 cut(s)	260, 285
SpeI	ACTAGT	2 cut(s)	1241, 2471
SsiI	CCGC	7 cut(s)	179, 237, 1602, 1767, 2127, 2301, 2433
SspI	AATATT	1 cut(s)	886
SspMI	CTAG	6 cut(s)	996, 1242, 1910, 1916, 2466, 2472
SstI	GAGCTC	1 cut(s)	32
StyD4I	CCNGG	5 cut(s)	466, 1088, 2238, 2324, 2348
StyI	CCWWGG	1 cut(s)	354
TaaI	ACNGT	7 cut(s)	50, 520, 579, 751, 1270, 1413, 1747
TaiI	ACGT	3 cut(s)	282, 931, 2524
TaqI	TCGA	6 cut(s)	90, 198, 217, 836, 1886, 2011
TaqII	GACCGA	1 cut(s)	1069
TatI	WGTACW	2 cut(s)	783, 1986
TauI	GCSGC	1 cut(s)	181
TfiI	GAWTC	7 cut(s)	87, 589, 934, 1334, 1352, 1421, 1678
Tru1I	TTAA	5 cut(s)	434, 602, 873, 1311, 1527
Tru9I	TTAA	5 cut(s)	434, 602, 873, 1311, 1527
TscAI	CASTG	5 cut(s)	39, 1201, 1970, 2029, 2202
TseFI	GTSAC	5 cut(s)	34, 93, 323, 979, 1264
TseI	GCWGC	7 cut(s)	239, 992, 1085, 1901, 1945, 2367, 2409
Tsp45I	GTSAC	5 cut(s)	34, 93, 323, 979, 1264
TspDTI	ATGAA	7 cut(s)	926, 1058, 1148, 1326, 1634, 1640, 1821
TspRI	CASTG	5 cut(s)	39, 1201, 1970, 2029, 2202
VpaK11BI	GGWCC	4 cut(s)	107, 443, 1598, 2236
XagI	CCTNNNNNAGG	1 cut(s)	288
XapI	RAATTY	5 cut(s)	368, 659, 854, 1531, 1808
XbaI	TCTAGA	1 cut(s)	1909
XceI	RCATGY	2 cut(s)	1592, 2213
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1699
XmnI	GAANNNNTTC	1 cut(s)	223
XspI	CTAG	6 cut(s)	996, 1242, 1910, 1916, 2466, 2472
Zsp2I	ATGCAT	1 cut(s)	483

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.