Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0014584

Physical Location & Seq

Genomic Coordinates Reverse (-)

31270060 .. 31282420

Gene Structure 12361 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00190870.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3117 bp

ATGGTGGTGGCGCCGCCCAAGTACGTGAGCTCGGTAGGCGATCTGAAGCGCCGGGAGTTGCAGAAGAAGAAAGCCAAGTCCGGAGGGTTCGAGTCTCTCAACCTAAGCCCCAATGTCTTCAATGCAATCAAGTGCAAGGGCTACAAAGTCCCCACACCCATTCAGCGCAAGACCATGCCCCTTATTCTCTCCGGAAACGACGTCGTCGCCATGGCCCGAACCGGTTCCGGCAAAACGGCGGCGTTTCTCATCCCCATGCTCGAGCGTTTGAAGGAGCACGTCCCCCAGGGCGGTGTTAGGGCTCTCATTCTCTCCCCCACTCGAGACTTGGCTCTTCAGACCCTCAAGTTTACCACAGAGCTCGCCCGTTTCATGGATCTTCGGATTAGTTTACTAGTTGGTGGTGATAGCATGGAAACTCAGTTTGAGGAGCTGGCTCAAAACCCGGATATTATAATTGCAACCCCTGGCAGGCTGATTCACCATTTAACTGAGATCGATGAGATGTCGCTTCGCTCTGTGGAGTATGTTGTTTTTGATGAGGCCGATTGTCTCTTTGGCATGGGTTTCGCTGAGCAATTGCATAAGATTCTTGCACAGCTGAGTGATAACCGTCAGACATTGCTCTTTAGTGCAACATTACCTAGCGCGCTCGCAGAGTTTGCAAAGGCTGAGAAGCATGCGGCAATACTTTATTTGGTAAGGGAGCATATTCGTTCCGATGAGCAGACGTTGATATTTGTATCCACTAAGCATCATGTGGAGTTCTTGAATATCTTGTTTAGAGAAGAGGGCATTGTGCCTTCTGTGTGTTATGGTGACATGGATCACGATGCTCGGAAAATTCATATATCAAAGTTTAAGCACAGAAAGACTATGTTGTTAATTGTCACTGATGTTGCAGCCAGGGGAATTGATATTCCATTGCTTGACAATGTTATCAATTGGGATTTTCCCCCAAAGCCTAAACTTTTTGTCCATAGAGTAGGTCGAGCTGCAAGGGCTGGTCGGACTGGCACTGCGTTCTCTCTTGTGACATCTGAAGACATACCTAACCTTTTAGATCTTCATCTGTTTCTTTCAAAACCGATTAGGCCGGCACCTACAGAGGAGGAGGTTTTACAGGATATGGATGGAGTAATGTCCAAGATTGATCAAGCAGTTGCAAATGGAGAAAGTATATATGGGCGTTTCCCCCAAACAGCTATTGATCTGGTTTCAGACAGGGTTAGAGAGATAATTGATTCTTCTTCAGAACTCAGCCTTTTGCTGAAAACTTGTGCAAATGCATTTCGCTTGTATTCAAAGACAAAGCCCTCCCCTTCAAGGGAATCTATTAAAAGAGCAAAAGATTTACCTCGGGAAGGGTTGCATCCAATATTTAAAAACACACTTGAGGGTGGAGAGTTGAAGGCATTGGCATTTTCTGAACGGTTGAAGACATTCAGACCTGAGCAGACCATTCTAGAAGCTGAAGGGAAAGCTGCTAAATCAAAGAATGTGAAGGGTCCTTCTCGTCAGTGGGTTGATGAAGATGAATCTGAGATTATTCCCTTAAAGAAAAAGAGAGAAAGGAAGGCAACGAGCTTCAAGGATGAGGATTACTTTATAAATTCCATACCCACAAATCATCATATGGAAGCGGGACTCTCAGTGAGAGGGAATGAGAACTTTGACTCTAACAGGTTGGAAGCTGCTGTTTTAGATCTAGTTGCAGATGATAATGTGGGCATGAAGAAACAGAAATCTGTATTTCATTGGGATAAGAGTGGGAAAAAGTACATAAAGCTAACTTCTCGTGACCGAGTGACTGCTAATGGCAAGGTAAAGACAGAGAGTGGTGCCAAAGGAAAACTTGAGAAAACTGGAATATACAATAAGTGGAAAGCACGTTCACACAACAAAGTATCTTTCAACGGCACTGACGAAGGGAATGCTGAGGAAGCTACAGGCAACCGGAGATGGCAGGGGAACAGGGGGAACCCACGGGGAGGCAGGAAGCAGCAGTCTATTCCTAATGCTCATGTGCGTTCTGAAATCAAAGACTTGGATCAAGTACGGAAGGAGAGGCAGAAAAAGGCAGATAGAATCCTTCATTTGAAGAGCAAATCTACAAAGGGGGCTCAGGCAATAATGGTCAAAGAAACTGAGTACTATGATGCACTCAATGTCACTCCCTCGGCTTCGGAAGAGGAAATTCGCAAGGCTTATTATCTCAAGGCAAGGCAAGTACATCCGGATAAAAATCCAAATGATCCTCAAGCTGCTGAAAGATTTCAGGCAAGTCCACTGCTTGTACTTGGGGAAGCCTACCAAGTTTTGAGTGATCCACTGCAAAGAGAAGCATATGATCGAAATGGAAAGCACTGCATATCTAGGGATACAATGCTTGACCCCACAGCTGTCTTTGCTTTTCTTTTCGGAAGTGAACTATTTGAGGATTACATAGGGCATCTAGCTGTAGCATCAATGGCTTCTTCTCAATTAGCTGGTGAAGGTGACAACCCTGAGAAAGCTGTCCAGAAAGAAAGAGAAGAAAAGCTAGCTAAGGCACTCAAAGATCTTCTTAATCAGTATGTGCGAGGTGATAAAGAAGGGTTCTTACTTCATGCTGAATCTGAAGCTAGAAGACTTTCTGATGCAGCCTTTGGAGTTGATATGCTACACACTATTGGCTATGTTTATTCAAGACAAGCAGCACAAGAGCTTGGAAAGAAAACCATATACCTAGGGGTACCATTTTTGGCTGAGTGGGTGCGCAACAAGGGACATTTCTGGAAGTCACAGATGACCGCAGCAAAAGGTGCATTCCAGCTGATGCAACTTCAAGAAGAAATCCGTCGGCAGTTTAAAGTGGATGGTAGTGGCCCTGAACATGATGTTGAATCACATATACGCTTAAACAAAGACACATTATTGAACTCATTGTGGAAATTGAATGTGGTTGACATTGAAGTGCTACGGGAAACTAATGTTCGAAAGGACGAACTTAGAGCTCGTGCTTTGGCACTAAAGCTTCTTGGAAAGATATTTCAGGGCATTGGAAGACTCTTCAGATGCCTCTGTAATCCAGCATATGATGTTGATGACGACGAGATTGTGTACAAAGGCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DBP10CT DEAD Helicase_C

Protein Analysis

1038

Amino Acids

116.81

Weight (kDa)

9.12

Isoelectric Point (pI)

34.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	52 - 220	2.6e-50	DEAD/DEAH box helicase
Helicase_C	PF00271	228 - 336	1.6e-23	Helicase conserved C-terminal domain
DBP10CT	PF08147	569 - 630	4.1e-09	DBP10CT (NUC160) domain
DnaJ	PF00226	717 - 784	1.1e-20	DnaJ domain
DnaJ-X	PF14308	841 - 1016	5.3e-41	X-domain of DnaJ-containing

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014584)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77030
fragaria_vesca	FvH4_2g15160 FvH4_2g15160
malus_domestica	MD07G1108300.v1.1
prunus_persica	Prupe.2G131100_v2.0.a1 Prupe.2G131100_v2.0.a1
pyrus_communis	pycom07g09270
rosa_chinensis	RchiOBHm_Chr6g0277821
rosa_laevigata	RLG00000013266
rosa_multiflora	Rmu_sc0001874.1_g000004
rosa_roxburghii	Rroxscaffold_7G00190870
rosa_rugosa	Rorug06G0111700
rosa_samantha	Rh6AG222500 Rh6BG226000 Rh6CG228500 Rh6DG219600
rosa_wichuraiana	Rw6G019430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	455, 1610
AatII	GACGTC	1 cut(s)	204
Acc16I	TGCGCA	1 cut(s)	2759
Acc65I	GGTACC	1 cut(s)	2734
AccB1I	GGYRCC	4 cut(s)	10, 1099, 1841, 2734
AccII	CGCG	1 cut(s)	650
AccIII	TCCGGA	3 cut(s)	80, 191, 2236
AciI	CCGC	6 cut(s)	14, 239, 291, 683, 1643, 2793
AclWI	GGATC	5 cut(s)	384, 834, 2058, 2249, 2321
AcsI	RAATTY	3 cut(s)	843, 1612, 2196
AcuI	CTGAAG	7 cut(s)	65, 320, 1062, 1236, 1494, 2640, 3037
AcyI	GRCGYC	2 cut(s)	11, 201
AfaI	GTAC	8 cut(s)	23, 1781, 2058, 2153, 2232, 2298, 2736, 3104
AfiI	CCNNNNNNNGG	7 cut(s)	290, 373, 471, 1326, 1327, 1364, 1991
AgeI	ACCGGT	1 cut(s)	221
AhlI	ACTAGT	1 cut(s)	394
AjiI	CACGTC	1 cut(s)	280
AjnI	CCWGG	3 cut(s)	285, 466, 905
AloI	GAACNNNNNNTCC	2 cut(s)	1876, 1908
Alw21I	GWGCWC	4 cut(s)	32, 279, 363, 2998
Alw26I	GTCTC	3 cut(s)	99, 318, 557
AlwI	GGATC	5 cut(s)	384, 834, 2058, 2249, 2321
Ama87I	CYCGRG	3 cut(s)	260, 321, 1359
Aor13HI	TCCGGA	3 cut(s)	80, 191, 2236
AoxI	GGCC	4 cut(s)	213, 543, 1094, 2866
ApeKI	GCWGC	9 cut(s)	902, 995, 1484, 1694, 2002, 2264, 2642, 2696, 2795
ApoI	RAATTY	3 cut(s)	843, 1612, 2196
AsiGI	ACCGGT	1 cut(s)	221
Asp700I	GAANNNNTTC	4 cut(s)	223, 1442, 2274, 2632
Asp718I	GGTACC	1 cut(s)	2734
AspA2I	CCTAGG	1 cut(s)	2728
AspLEI	GCGC	6 cut(s)	13, 51, 168, 650, 652, 2760
AspS9I	GGNCC	3 cut(s)	214, 1508, 2867
AsuC2I	CCSGG	2 cut(s)	53, 446
AsuHPI	GGTGA	6 cut(s)	416, 473, 830, 2504, 2510, 2597
AsuII	TTCGAA	1 cut(s)	2977
AsuNHI	GCTAGC	1 cut(s)	2542
AvaI	CYCGRG	3 cut(s)	260, 321, 1359
AvaII	GGWCC	1 cut(s)	1508
AvrII	CCTAGG	1 cut(s)	2728
BanI	GGYRCC	4 cut(s)	10, 1099, 1841, 2734
BanII	GRGCYC	5 cut(s)	32, 304, 363, 2125, 2998
BarI	GAAGNNNNNNTAC	2 cut(s)	2586, 2618
BauI	CACGAG	2 cut(s)	1797, 2997
BbsI	GAAGAC	5 cut(s)	109, 1050, 1445, 2635, 3052
Bbv12I	GWGCWC	4 cut(s)	32, 279, 363, 2998
BbvCI	CCTCAGC	1 cut(s)	1938
BbvI	GCAGC	9 cut(s)	914, 982, 1471, 1681, 2014, 2251, 2654, 2708, 2807
BccI	CCATC	3 cut(s)	1127, 1956, 2852
BceAI	ACGGC	2 cut(s)	252, 1933
BcgI	CGANNNNNNTGC	4 cut(s)	550, 584, 1916, 1950
BciT130I	CCWGG	3 cut(s)	287, 468, 907
BciVI	GTATCC	2 cut(s)	754, 2374
BclI	TGATCA	1 cut(s)	1153
BcnI	CCSGG	2 cut(s)	53, 446
BcoDI	GTCTC	3 cut(s)	99, 318, 557
BcuI	ACTAGT	1 cut(s)	394
BfaI	CTAG	9 cut(s)	395, 645, 1466, 1709, 2376, 2456, 2543, 2625, 2729
BfmI	CTRYAG	3 cut(s)	1104, 1947, 2460
BfoI	RGCGCY	2 cut(s)	14, 52
BfuI	GTATCC	2 cut(s)	754, 2374
BglII	AGATCT	3 cut(s)	1063, 1705, 2560
BlnI	CCTAGG	1 cut(s)	2728
BlpI	GCTNAGC	1 cut(s)	573
BmcAI	AGTACT	1 cut(s)	2153
Bme1390I	CCNGG	5 cut(s)	53, 287, 446, 468, 907
Bme18I	GGWCC	1 cut(s)	1508
BmeT110I	CYCGRG	3 cut(s)	260, 321, 1359
BmgBI	CACGTC	1 cut(s)	280
BmgT120I	GGNCC	3 cut(s)	214, 1508, 2867
BmiI	GGNNCC	7 cut(s)	12, 226, 1101, 1509, 1843, 1982, 2736
BmrFI	CCNGG	5 cut(s)	53, 287, 446, 468, 907
BmsI	GCATC	9 cut(s)	763, 823, 1381, 2149, 2461, 2474, 2629, 2808, 3047
BmtI	GCTAGC	1 cut(s)	2546
BpiI	GAAGAC	5 cut(s)	109, 1050, 1445, 2635, 3052
Bpu10I	CCTNAGC	5 cut(s)	104, 1452, 1938, 2124, 2547
Bpu1102I	GCTNAGC	1 cut(s)	573
Bpu14I	TTCGAA	1 cut(s)	2977
BpuEI	CTTGAG	5 cut(s)	329, 1415, 1877, 2201, 2244
BpuMI	CCSGG	2 cut(s)	53, 446
Bsa29I	ATCGAT	1 cut(s)	498
BsaAI	YACGTR	1 cut(s)	25
BsaBI	GATNNNNATC	2 cut(s)	1068, 2244
BsaHI	GRCGYC	2 cut(s)	11, 201
BsaJI	CCNNGG	9 cut(s)	210, 285, 286, 466, 906, 1358, 1985, 2178, 2728
BsaWI	WCCGGW	5 cut(s)	80, 191, 221, 1956, 2236
Bsc4I	CCNNNNNNNGG	7 cut(s)	290, 373, 471, 1326, 1327, 1364, 1991
Bse118I	RCCGGY	2 cut(s)	221, 1096
Bse1I	ACTGG	2 cut(s)	1018, 1870
Bse3DI	GCAATG	2 cut(s)	620, 923
Bse8I	GATNNNNATC	2 cut(s)	1068, 2244
BseAI	TCCGGA	3 cut(s)	80, 191, 2236
BseBI	CCWGG	3 cut(s)	287, 468, 907
BseCI	ATCGAT	1 cut(s)	498
BseDI	CCNNGG	9 cut(s)	210, 285, 286, 466, 906, 1358, 1985, 2178, 2728
BseGI	GGATG	6 cut(s)	249, 1138, 1372, 1600, 2233, 2863
BseJI	GATNNNNATC	2 cut(s)	1068, 2244
BseLI	CCNNNNNNNGG	7 cut(s)	290, 373, 471, 1326, 1327, 1364, 1991
BseMI	GCAATG	2 cut(s)	620, 923
BseNI	ACTGG	2 cut(s)	1018, 1870
BsePI	GCGCGC	1 cut(s)	648
BseRI	GAGGAG	3 cut(s)	443, 1124, 1127
BseXI	GCAGC	9 cut(s)	914, 982, 1471, 1681, 2014, 2251, 2654, 2708, 2807
Bsh1236I	CGCG	1 cut(s)	650
BshFI	GGCC	4 cut(s)	215, 545, 1096, 2868
BshNI	GGYRCC	4 cut(s)	10, 1099, 1841, 2734
BshTI	ACCGGT	1 cut(s)	221
BshVI	ATCGAT	1 cut(s)	498
BsiHKAI	GWGCWC	4 cut(s)	32, 279, 363, 2998
BsiHKCI	CYCGRG	3 cut(s)	260, 321, 1359
BsiSI	CCGG	9 cut(s)	52, 81, 192, 222, 228, 446, 1097, 1957, 2237
BslFI	GGGAC	4 cut(s)	134, 266, 1659, 2781
BslI	CCNNNNNNNGG	7 cut(s)	290, 373, 471, 1326, 1327, 1364, 1991
BsmAI	GTCTC	3 cut(s)	99, 318, 557
BsmFI	GGGAC	4 cut(s)	134, 266, 1659, 2781
BsmI	GAATGC	2 cut(s)	1939, 2807
BsnI	GGCC	4 cut(s)	215, 545, 1096, 2868
BsoBI	CYCGRG	3 cut(s)	260, 321, 1359
Bsp119I	TTCGAA	1 cut(s)	2977
Bsp1286I	GDGCHC	6 cut(s)	32, 279, 304, 363, 2125, 2998
Bsp13I	TCCGGA	3 cut(s)	80, 191, 2236
Bsp1407I	TGTACA	1 cut(s)	3102
Bsp1720I	GCTNAGC	1 cut(s)	573
Bsp19I	CCATGG	1 cut(s)	210
BspACI	CCGC	6 cut(s)	14, 239, 291, 683, 1643, 2793
BspANI	GGCC	4 cut(s)	215, 545, 1096, 2868
BspDI	ATCGAT	1 cut(s)	498
BspEI	TCCGGA	3 cut(s)	80, 191, 2236
BspFNI	CGCG	1 cut(s)	650
BspLI	GGNNCC	7 cut(s)	12, 226, 1101, 1509, 1843, 1982, 2736
BspOI	GCTAGC	1 cut(s)	2546
BspPI	GGATC	5 cut(s)	384, 834, 2058, 2249, 2321
BspQI	GCTCTTC	2 cut(s)	339, 2096
BspT104I	TTCGAA	1 cut(s)	2977
BspT107I	GGYRCC	4 cut(s)	10, 1099, 1841, 2734
BsrDI	GCAATG	2 cut(s)	620, 923
BsrFI	RCCGGY	2 cut(s)	221, 1096
BsrGI	TGTACA	1 cut(s)	3102
BsrI	ACTGG	2 cut(s)	1018, 1870
BssAI	RCCGGY	2 cut(s)	221, 1096
BssECI	CCNNGG	9 cut(s)	210, 285, 286, 466, 906, 1358, 1985, 2178, 2728
BssHII	GCGCGC	1 cut(s)	648
BssNI	GRCGYC	2 cut(s)	11, 201
BssSI	CACGAG	2 cut(s)	1797, 2997
BssT1I	CCWWGG	2 cut(s)	210, 2728
Bst2BI	CACGAG	2 cut(s)	1797, 2997
Bst2UI	CCWGG	3 cut(s)	287, 468, 907
Bst4CI	ACNGT	2 cut(s)	614, 1434
Bst6I	CTCTTC	5 cut(s)	339, 783, 2096, 2184, 3056
BstACI	GRCGYC	2 cut(s)	11, 201
BstAPI	GCANNNNNTGC	2 cut(s)	662, 2804
BstAUI	TGTACA	1 cut(s)	3102
BstBAI	YACGTR	1 cut(s)	25
BstBI	TTCGAA	1 cut(s)	2977
BstC8I	GCNNGC	8 cut(s)	363, 435, 473, 650, 654, 681, 1098, 2544
BstDSI	CCRYGG	2 cut(s)	210, 1985
BstENI	CCTNNNNNAGG	1 cut(s)	1362
BstF5I	GGATG	6 cut(s)	249, 1138, 1372, 1600, 2233, 2863
BstFNI	CGCG	1 cut(s)	650
BstH2I	RGCGCY	2 cut(s)	14, 52
BstHHI	GCGC	6 cut(s)	13, 51, 168, 650, 652, 2760
BstMAI	GTCTC	3 cut(s)	99, 318, 557
BstMWI	GCNNNNNNNGC	7 cut(s)	36, 662, 1001, 1943, 2408, 2471, 2804
BstNI	CCWGG	3 cut(s)	287, 468, 907
BstNSI	RCATGY	1 cut(s)	683
BstSCI	CCNGG	5 cut(s)	51, 285, 444, 466, 905
BstSFI	CTRYAG	3 cut(s)	1104, 1947, 2460
BstUI	CGCG	1 cut(s)	650
BstV1I	GCAGC	9 cut(s)	914, 982, 1471, 1681, 2014, 2251, 2654, 2708, 2807
BstV2I	GAAGAC	5 cut(s)	109, 1050, 1445, 2635, 3052
BstX2I	RGATCY	4 cut(s)	376, 1063, 1705, 2560
BstYI	RGATCY	4 cut(s)	376, 1063, 1705, 2560
Bsu15I	ATCGAT	1 cut(s)	498
BsuI	GTATCC	2 cut(s)	754, 2374
BsuRI	GGCC	4 cut(s)	215, 545, 1096, 2868
BsuTUI	ATCGAT	1 cut(s)	498
BtgI	CCRYGG	2 cut(s)	210, 1985
BtrI	CACGTC	1 cut(s)	280
BtsCI	GGATG	6 cut(s)	249, 1138, 1372, 1600, 2233, 2863
BtsI	GCAGTG	4 cut(s)	1017, 2288, 2330, 2365
BtsIMutI	CAGTG	8 cut(s)	891, 1017, 1526, 1659, 1920, 2288, 2330, 2365
Cac8I	GCNNGC	8 cut(s)	363, 435, 473, 650, 654, 681, 1098, 2544
CfoI	GCGC	6 cut(s)	13, 51, 168, 650, 652, 2760
Cfr10I	RCCGGY	2 cut(s)	221, 1096
Cfr13I	GGNCC	3 cut(s)	214, 1508, 2867
ClaI	ATCGAT	1 cut(s)	498
Csp6I	GTAC	8 cut(s)	22, 1780, 2057, 2152, 2231, 2297, 2735, 3103
CspAI	ACCGGT	1 cut(s)	221
CviQI	GTAC	8 cut(s)	22, 1780, 2057, 2152, 2231, 2297, 2735, 3103
DinI	GGCGCC	1 cut(s)	12
DraI	TTTAAA	2 cut(s)	1384, 2851
Eam1104I	CTCTTC	5 cut(s)	339, 783, 2096, 2184, 3056
EarI	CTCTTC	5 cut(s)	339, 783, 2096, 2184, 3056
Ecl136II	GAGCTC	3 cut(s)	30, 361, 2996
Eco130I	CCWWGG	2 cut(s)	210, 2728
Eco24I	GRGCYC	5 cut(s)	32, 304, 363, 2125, 2998
Eco47I	GGWCC	1 cut(s)	1508
Eco53kI	GAGCTC	3 cut(s)	30, 361, 2996
Eco57I	CTGAAG	7 cut(s)	65, 320, 1062, 1236, 1494, 2640, 3037
Eco88I	CYCGRG	3 cut(s)	260, 321, 1359
EcoICRI	GAGCTC	3 cut(s)	30, 361, 2996
EcoNI	CCTNNNNNAGG	1 cut(s)	1362
EcoO109I	RGGNCCY	1 cut(s)	1508
EcoRII	CCWGG	3 cut(s)	285, 466, 905
EcoT14I	CCWWGG	2 cut(s)	210, 2728
EcoT22I	ATGCAT	1 cut(s)	1291
EcoT38I	GRGCYC	5 cut(s)	32, 304, 363, 2125, 2998
EgeI	GGCGCC	1 cut(s)	12
EheI	GGCGCC	1 cut(s)	12
ErhI	CCWWGG	2 cut(s)	210, 2728
FalI	AAGNNNNNCTT	4 cut(s)	1839, 1871, 2550, 2582
FaqI	GGGAC	4 cut(s)	134, 266, 1659, 2781
FauI	CCCGC	1 cut(s)	1636
FauNDI	CATATG	3 cut(s)	1635, 2347, 3076
FbaI	TGATCA	1 cut(s)	1153
FokI	GGATG	6 cut(s)	236, 1145, 1359, 1607, 2220, 2870
FriOI	GRGCYC	5 cut(s)	32, 304, 363, 2125, 2998
FspBI	CTAG	9 cut(s)	395, 645, 1466, 1709, 2376, 2456, 2543, 2625, 2729
FspI	TGCGCA	1 cut(s)	2759
GlaI	GCGC	6 cut(s)	12, 50, 167, 649, 651, 2759
HaeII	RGCGCY	2 cut(s)	14, 52
HaeIII	GGCC	4 cut(s)	215, 545, 1096, 2868
HapII	CCGG	9 cut(s)	52, 81, 192, 222, 228, 446, 1097, 1957, 2237
HhaI	GCGC	6 cut(s)	13, 51, 168, 650, 652, 2760
Hin1I	GRCGYC	2 cut(s)	11, 201
Hin6I	GCGC	6 cut(s)	11, 49, 166, 648, 650, 2758
HinP1I	GCGC	6 cut(s)	11, 49, 166, 648, 650, 2758
HincII	GTYRAC	1 cut(s)	2947
HindII	GTYRAC	1 cut(s)	2947
HindIII	AAGCTT	1 cut(s)	3014
HpaII	CCGG	9 cut(s)	52, 81, 192, 222, 228, 446, 1097, 1957, 2237
HphI	GGTGA	6 cut(s)	416, 473, 830, 2504, 2510, 2597
Hpy166II	GTNNAC	7 cut(s)	351, 392, 1895, 2288, 2429, 2947, 3103
Hpy8I	GTNNAC	7 cut(s)	351, 392, 1895, 2288, 2429, 2947, 3103
Hpy99I	CGWCG	5 cut(s)	203, 206, 209, 2844, 3095
HpyCH4III	ACNGT	2 cut(s)	614, 1434
HpyCH4IV	ACGT	5 cut(s)	24, 201, 279, 731, 1891
HpyF10VI	GCNNNNNNNGC	7 cut(s)	36, 662, 1001, 1943, 2408, 2471, 2804
HpySE526I	ACGT	5 cut(s)	24, 201, 279, 731, 1891
Hsp92I	GRCGYC	2 cut(s)	11, 201
HspAI	GCGC	6 cut(s)	11, 49, 166, 648, 650, 2758
KasI	GGCGCC	1 cut(s)	10
Kpn2I	TCCGGA	3 cut(s)	80, 191, 2236
KpnI	GGTACC	1 cut(s)	2738
KroI	GCCGGC	1 cut(s)	1096
KroNI	GCCGGC	1 cut(s)	1098
Ksp22I	TGATCA	1 cut(s)	1153
LguI	GCTCTTC	2 cut(s)	339, 2096
LmnI	GCTCC	3 cut(s)	274, 430, 706
Lsp1109I	GCAGC	9 cut(s)	914, 982, 1471, 1681, 2014, 2251, 2654, 2708, 2807
LweI	GCATC	9 cut(s)	763, 823, 1381, 2149, 2461, 2474, 2629, 2808, 3047
MaeI	CTAG	9 cut(s)	395, 645, 1466, 1709, 2376, 2456, 2543, 2625, 2729
MaeII	ACGT	5 cut(s)	24, 201, 279, 731, 1891
MaeIII	GTNAC	8 cut(s)	818, 889, 1033, 1799, 1807, 2170, 2498, 2781
MfeI	CAATTG	2 cut(s)	578, 943
MflI	RGATCY	4 cut(s)	376, 1063, 1705, 2560
MhlI	GDGCHC	6 cut(s)	32, 279, 304, 363, 2125, 2998
Mly113I	GGCGCC	1 cut(s)	11
MlyI	GAGTC	4 cut(s)	101, 1641, 1670, 3042
MmeI	TCCRAC	2 cut(s)	989, 1668
Mph1103I	ATGCAT	1 cut(s)	1291
MroI	TCCGGA	3 cut(s)	80, 191, 2236
MroNI	GCCGGC	1 cut(s)	1096
MroXI	GAANNNNTTC	4 cut(s)	223, 1442, 2274, 2632
MseI	TTAA	9 cut(s)	488, 861, 884, 1338, 1383, 1556, 2568, 2850, 2900
MslI	CAYNNNNRTG	2 cut(s)	254, 2954
MspA1I	CMGCKG	3 cut(s)	601, 2402, 2815
MspI	CCGG	9 cut(s)	52, 81, 192, 222, 228, 446, 1097, 1957, 2237
MspR9I	CCNGG	5 cut(s)	53, 287, 446, 468, 907
MunI	CAATTG	2 cut(s)	578, 943
Mva1269I	GAATGC	2 cut(s)	1939, 2807
MvaI	CCWGG	3 cut(s)	287, 468, 907
MvnI	CGCG	1 cut(s)	650
MwoI	GCNNNNNNNGC	7 cut(s)	36, 662, 1001, 1943, 2408, 2471, 2804
NaeI	GCCGGC	1 cut(s)	1098
NarI	GGCGCC	1 cut(s)	11
NciI	CCSGG	2 cut(s)	53, 446
NcoI	CCATGG	1 cut(s)	210
NdeI	CATATG	3 cut(s)	1635, 2347, 3076
NgoMIV	GCCGGC	1 cut(s)	1096
NheI	GCTAGC	1 cut(s)	2542
NlaIV	GGNNCC	7 cut(s)	12, 226, 1101, 1509, 1843, 1982, 2736
NmeAIII	GCCGAG	1 cut(s)	2159
NmuCI	GTSAC	8 cut(s)	818, 889, 1033, 1799, 1807, 2170, 2498, 2781
NsbI	TGCGCA	1 cut(s)	2759
NsiI	ATGCAT	1 cut(s)	1291
NspI	RCATGY	1 cut(s)	683
NspV	TTCGAA	1 cut(s)	2977
PaeI	GCATGC	1 cut(s)	683
PaeR7I	CTCGAG	2 cut(s)	260, 321
PasI	CCCWGGG	1 cut(s)	286
PauI	GCGCGC	1 cut(s)	648
PciSI	GCTCTTC	2 cut(s)	339, 2096
PcsI	WCGNNNNNNNCGW	1 cut(s)	1923
PctI	GAATGC	2 cut(s)	1939, 2807
PdiI	GCCGGC	1 cut(s)	1098
PdmI	GAANNNNTTC	4 cut(s)	223, 1442, 2274, 2632
PfeI	GAWTC	8 cut(s)	478, 589, 1244, 1331, 1538, 2088, 2615, 2885
PflFI	GACNNNGTC	1 cut(s)	203
PinAI	ACCGGT	1 cut(s)	221
PleI	GAGTC	4 cut(s)	100, 1641, 1670, 3042
PluTI	GGCGCC	1 cut(s)	14
PpsI	GAGTC	4 cut(s)	100, 1641, 1670, 3042
Ppu21I	YACGTR	1 cut(s)	25
PpuMI	RGGWCCY	1 cut(s)	1508
PsiI	TTATAA	2 cut(s)	455, 1610
Psp124BI	GAGCTC	3 cut(s)	32, 363, 2998
Psp5II	RGGWCCY	1 cut(s)	1508
Psp6I	CCWGG	3 cut(s)	285, 466, 905
PspGI	CCWGG	3 cut(s)	285, 466, 905
PspN4I	GGNNCC	7 cut(s)	12, 226, 1101, 1509, 1843, 1982, 2736
PspPI	GGNCC	3 cut(s)	214, 1508, 2867
PspPPI	RGGWCCY	1 cut(s)	1508
PspXI	VCTCGAGB	1 cut(s)	260
PsuI	RGATCY	4 cut(s)	376, 1063, 1705, 2560
PsyI	GACNNNGTC	1 cut(s)	203
PteI	GCGCGC	1 cut(s)	648
PvuII	CAGCTG	3 cut(s)	601, 2402, 2815
RsaI	GTAC	8 cut(s)	23, 1781, 2058, 2153, 2232, 2298, 2736, 3104
RsaNI	GTAC	8 cut(s)	22, 1780, 2057, 2152, 2231, 2297, 2735, 3103
RseI	CAYNNNNRTG	2 cut(s)	254, 2954
SacI	GAGCTC	3 cut(s)	32, 363, 2998
SapI	GCTCTTC	2 cut(s)	339, 2096
SaqAI	TTAA	9 cut(s)	488, 861, 884, 1338, 1383, 1556, 2568, 2850, 2900
Sau96I	GGNCC	3 cut(s)	214, 1508, 2867
ScaI	AGTACT	1 cut(s)	2153
SchI	GAGTC	4 cut(s)	101, 1641, 1670, 3042
ScrFI	CCNGG	5 cut(s)	53, 287, 446, 468, 907
SduI	GDGCHC	6 cut(s)	32, 279, 304, 363, 2125, 2998
SfaNI	GCATC	9 cut(s)	763, 823, 1381, 2149, 2461, 2474, 2629, 2808, 3047
SfcI	CTRYAG	3 cut(s)	1104, 1947, 2460
SfoI	GGCGCC	1 cut(s)	12
Sfr274I	CTCGAG	2 cut(s)	260, 321
SfuI	TTCGAA	1 cut(s)	2977
SinI	GGWCC	1 cut(s)	1508
SlaI	CTCGAG	2 cut(s)	260, 321
SmiMI	CAYNNNNRTG	2 cut(s)	254, 2954
SmlI	CTYRAG	7 cut(s)	260, 321, 344, 1394, 1856, 2216, 2259
SmoI	CTYRAG	7 cut(s)	260, 321, 344, 1394, 1856, 2216, 2259
SpeI	ACTAGT	1 cut(s)	394
SphI	GCATGC	1 cut(s)	683
SsiI	CCGC	6 cut(s)	14, 239, 291, 683, 1643, 2793
SspDI	GGCGCC	1 cut(s)	10
SspI	AATATT	1 cut(s)	1380
SspMI	CTAG	9 cut(s)	395, 645, 1466, 1709, 2376, 2456, 2543, 2625, 2729
SstI	GAGCTC	3 cut(s)	32, 363, 2998
StyD4I	CCNGG	5 cut(s)	51, 285, 444, 466, 905
StyI	CCWWGG	2 cut(s)	210, 2728
TaaI	ACNGT	2 cut(s)	614, 1434
TaiI	ACGT	5 cut(s)	27, 204, 282, 734, 1894
TaqI	TCGA	7 cut(s)	90, 261, 322, 498, 991, 2353, 2977
TaqII	GACCGA	1 cut(s)	1818
TatI	WGTACW	5 cut(s)	1779, 2151, 2230, 2296, 3102
TauI	GCSGC	3 cut(s)	16, 242, 686
TfiI	GAWTC	8 cut(s)	478, 589, 1244, 1331, 1538, 2088, 2615, 2885
Tru1I	TTAA	9 cut(s)	488, 861, 884, 1338, 1383, 1556, 2568, 2850, 2900
Tru9I	TTAA	9 cut(s)	488, 861, 884, 1338, 1383, 1556, 2568, 2850, 2900
TscAI	CASTG	8 cut(s)	898, 1024, 1526, 1659, 1927, 2295, 2337, 2372
TseFI	GTSAC	8 cut(s)	818, 889, 1033, 1799, 1807, 2170, 2498, 2781
TseI	GCWGC	9 cut(s)	902, 995, 1484, 1694, 2002, 2264, 2642, 2696, 2795
Tsp45I	GTSAC	8 cut(s)	818, 889, 1033, 1799, 1807, 2170, 2498, 2781
TspDTI	ATGAA	9 cut(s)	361, 836, 1058, 1545, 1551, 1745, 1748, 2084, 2597
TspGWI	ACGGA	2 cut(s)	2074, 2828
TspRI	CASTG	8 cut(s)	898, 1024, 1526, 1659, 1927, 2295, 2337, 2372
Tth111I	GACNNNGTC	1 cut(s)	203
VpaK11BI	GGWCC	1 cut(s)	1508
XagI	CCTNNNNNAGG	1 cut(s)	1362
XapI	RAATTY	3 cut(s)	843, 1612, 2196
XbaI	TCTAGA	1 cut(s)	1465
XceI	RCATGY	1 cut(s)	683
XcmI	CCANNNNNNNNNTGG	1 cut(s)	325
XhoI	CTCGAG	2 cut(s)	260, 321
XmaJI	CCTAGG	1 cut(s)	2728
XmnI	GAANNNNTTC	4 cut(s)	223, 1442, 2274, 2632
XspI	CTAG	9 cut(s)	395, 645, 1466, 1709, 2376, 2456, 2543, 2625, 2729
ZraI	GACGTC	1 cut(s)	202
ZrmI	AGTACT	1 cut(s)	2153
Zsp2I	ATGCAT	1 cut(s)	1291

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.