AT1G77140

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004658
Physical Location & Seq
Forward (+)
28983934 .. 28988035
4102 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G77140.1

Sequence Viewer

Length: 1710 bp
ATGGTTTTGGTTACGTCTGTGCGTGATTACATTAACCGGATGCTTCAGGACATCTCCGGCATGAAGGTTCTCATACTCGACTCCGAAACGGTTAGCAATGTAAGCATTGTGTATTCACAGTCAGAGCTGCTTCAGAAAGAAGTGTTTCTGGTGGAGATGATAGATTCAATATCTGTGTCAAAAGAATCGATGTCACATCTGAAAGCTGTTTATTTCATCCGTCCAACTTCAGATAATATTCAGAAATTACGATACCAGCTTGCGAATCCTAGATTTGGAGAATACCATTTATTTTTCTCCAATCTGTTGAAGGATACTCAGATTCATATTTTGGCTGATTCCGATGAGCAGGAAGTTGTACAGCAAGTTCAGGAGTATTATGCAGACTTTGTTTCTGGTGATCCATATCATTTCACATTGAATATGGCATCAAATCATCTATATATGATCCCCGCAGTTGTCGATCCCTCTGGTTTGCAACGCTTTTCCGATCGGGTTGTTGATGGAATTGCGGCAGTGTTTCTGGCGTTGAAACGAAGACCTGTCATCAGATATCAGAGGACCTCTGATACTGCAAAAAGGATTGCACACGAAACAGCTAAATTGATGTATCAGCATGAAAGCGCTCTTTTTGACTTTAGACGGACTGAAAGCTCTCCGTTGCTACTTGTAATTGACAGAAGAGATGACCCGGTTACCCCATTGCTGAATCAGTGGACATATCAGGCAATGGTGCACGAACTCATAGGACTTCAAGATAATAAAGTGGACTTGAAATCCATTGGAAGTCTTCCAAAAGATCAGCAAGTGGAGGTGGTTTTATCATCAGAACAAGATGCATTTTTCAAATCTAACATGTATGAAAATTTTGGGGATATTGGAATGAACATCAAGCGAATGGTAGATGACTTTCAGCAGGTGGCCAAGAGTAACCAAAATATCCAGACAGTAGAGGATATGGCCAGATTTGTTGACAACTACCCTGAGTACAAAAAGATGCAAGGCAACGTTTCAAAGCATGTAACTCTGGTTACTGAAATGAGCAAGCTAGTTGAAGCAAGGAAACTGATGACGGTTTCACAAACAGAGCAGGACTTGGCTTGCAATGGTGGCCAAGGAGCTGCATATGAGGCAGTTACGGATCTCTTAAATAATGAAAGTGTGTCTGACATCGACCGGCTGCGTTTGGTAATGCTATATGCTTTGCGCTATGAGAAAGAAAATCCTGTTCAGTTGATGCAATTGTTCAACAAATTGGCTTCACGGTCTCCCAAGTACAAACCAGGGCTAGTTCAATTTCTCTTGAAACAAGCCGGTGTGGAGAAGCGCACTGGTGATCTATTTGGAAACAGAGATCTTTTGAATATAGCACGTAACATGGCTCGTGGACTTAAGGGTGTTGAGAATGTCTACACTCAACATCAGCCTCTTCTGTTTCAAACAATGGAGAGCATAACTAGAGGGAGATTACGAGATGTGGATTACCCATTTGTTGGAGATCATTTTCAACAGGGACGGCCACAAGAGGTGGTTATTTTTATGGTTGGTGGAACAACATACGAGGAATCACGCTCTGTTGCTCTACAGAATGCCACCAACTCCGGGGTTCGGTTTATTCTCGGCGGCACCGCAGTGCTTAATTCCAAGAGATTTCTAAAGGACTTGGAAGAAGCACAAAGGATATCAAGATCAGGTAGCCATATGGTGTGA

Protein Analysis

569

Amino Acids

64.94

Weight (kDa)

6.22

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 22 - 554 1.6e-123 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 907
Acc36I ACCTGC 1 cut(s) 907
AccB1I GGYRCC 1 cut(s) 1625
AccB7I CCANNNNNTGG 1 cut(s) 1493
AccI GTMKAC 1 cut(s) 1410
AciI CCGC 4 cut(s) 453, 512, 1623, 1629
AclI AACGTT 1 cut(s) 1008
AclWI GGATC 4 cut(s) 395, 442, 458, 1149
AcoI YGGCCR 4 cut(s) 921, 960, 1111, 1517
AcsI RAATTY 1 cut(s) 865
AcuI CTGAAG 3 cut(s) 29, 116, 213
AfaI GTAC 3 cut(s) 360, 989, 1277
AfeI AGCGCT 1 cut(s) 625
AfiI CCNNNNNNNGG 4 cut(s) 275, 1493, 1602, 1608
AflII CTTAAG 1 cut(s) 1391
AflIII ACRYGT 1 cut(s) 855
AjnI CCWGG 1 cut(s) 1282
AleI CACNNNNGTG 1 cut(s) 1631
AluBI AGCT 7 cut(s) 127, 206, 259, 599, 654, 1048, 1121
AluI AGCT 7 cut(s) 127, 206, 259, 599, 654, 1048, 1121
Alw21I GWGCWC 1 cut(s) 738
Alw26I GTCTC 1 cut(s) 1272
Alw44I GTGCAC 1 cut(s) 734
AlwI GGATC 4 cut(s) 395, 442, 458, 1149
Aor51HI AGCGCT 1 cut(s) 625
AoxI GGCC 4 cut(s) 921, 960, 1111, 1517
ApaLI GTGCAC 1 cut(s) 734
ApeKI GCWGC 3 cut(s) 127, 1121, 1180
ApoI RAATTY 1 cut(s) 865
Asp700I GAANNNNTTC 1 cut(s) 144
AspLEI GCGC 3 cut(s) 626, 1209, 1329
AspS9I GGNCC 1 cut(s) 561
AsuC2I CCSGG 2 cut(s) 692, 1603
AsuHPI GGTGA 2 cut(s) 410, 1346
AvaII GGWCC 1 cut(s) 561
BaeGI GKGCMC 1 cut(s) 738
BalI TGGCCA 3 cut(s) 923, 962, 1113
BanI GGYRCC 1 cut(s) 1625
BauI CACGAG 1 cut(s) 1383
BbsI GAAGAC 2 cut(s) 544, 782
Bbv12I GWGCWC 1 cut(s) 738
BbvI GCAGC 3 cut(s) 114, 1108, 1167
BccI CCATC 1 cut(s) 497
BceAI ACGGC 1 cut(s) 1532
BciT130I CCWGG 1 cut(s) 1284
BciVI GTATCC 1 cut(s) 307
BcnI CCSGG 2 cut(s) 692, 1603
BcoDI GTCTC 1 cut(s) 1272
BfaI CTAG 4 cut(s) 270, 1049, 1289, 1458
BfmI CTRYAG 1 cut(s) 1583
BfoI RGCGCY 1 cut(s) 627
BfrI CTTAAG 1 cut(s) 1391
BfuAI ACCTGC 1 cut(s) 907
BfuI GTATCC 1 cut(s) 307
BglII AGATCT 1 cut(s) 1354
BisI GCNGC 5 cut(s) 128, 513, 1122, 1181, 1624
BlsI GCNGC 5 cut(s) 129, 514, 1123, 1182, 1625
Bme1390I CCNGG 3 cut(s) 692, 1284, 1603
Bme18I GGWCC 1 cut(s) 561
BmgT120I GGNCC 1 cut(s) 561
BmiI GGNNCC 1 cut(s) 1627
BmrFI CCNGG 3 cut(s) 692, 1284, 1603
BmsI GCATC 5 cut(s) 30, 437, 826, 987, 1227
BpiI GAAGAC 2 cut(s) 544, 782
BpuMI CCSGG 2 cut(s) 692, 1603
Bsa29I ATCGAT 1 cut(s) 188
BsaAI YACGTR 1 cut(s) 1373
BsaBI GATNNNNATC 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 1272
BsaJI CCNNGG 3 cut(s) 1114, 1283, 1602
BsaWI WCCGGW 1 cut(s) 36
Bsc4I CCNNNNNNNGG 4 cut(s) 275, 1493, 1602, 1608
Bse118I RCCGGY 2 cut(s) 1176, 1313
Bse1I ACTGG 1 cut(s) 1336
Bse3DI GCAATG 4 cut(s) 103, 701, 735, 1111
Bse8I GATNNNNATC 1 cut(s) 405
BseBI CCWGG 1 cut(s) 1284
BseCI ATCGAT 1 cut(s) 188
BseDI CCNNGG 3 cut(s) 1114, 1283, 1602
BseGI GGATG 2 cut(s) 45, 216
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 4 cut(s) 275, 1493, 1602, 1608
BseMI GCAATG 4 cut(s) 103, 701, 735, 1111
BseMII CTCAG 2 cut(s) 332, 975
BseNI ACTGG 1 cut(s) 1336
BseSI GKGCMC 1 cut(s) 738
BseXI GCAGC 3 cut(s) 114, 1108, 1167
Bsh1285I CGRYCG 2 cut(s) 493, 1177
BshFI GGCC 4 cut(s) 923, 962, 1113, 1519
BshNI GGYRCC 1 cut(s) 1625
BshVI ATCGAT 1 cut(s) 188
BsiEI CGRYCG 2 cut(s) 493, 1177
BsiHKAI GWGCWC 1 cut(s) 738
BsiSI CCGG 6 cut(s) 37, 57, 692, 1177, 1314, 1602
BslFI GGGAC 1 cut(s) 1527
BslI CCNNNNNNNGG 4 cut(s) 275, 1493, 1602, 1608
BsmAI GTCTC 1 cut(s) 1272
BsmFI GGGAC 1 cut(s) 1527
BsmI GAATGC 1 cut(s) 1594
BsnI GGCC 4 cut(s) 923, 962, 1113, 1519
Bso31I GGTCTC 1 cut(s) 1272
Bsp1286I GDGCHC 1 cut(s) 738
Bsp1407I TGTACA 1 cut(s) 358
BspACI CCGC 4 cut(s) 453, 512, 1623, 1629
BspANI GGCC 4 cut(s) 923, 962, 1113, 1519
BspCNI CTCAG 2 cut(s) 331, 976
BspDI ATCGAT 1 cut(s) 188
BspLI GGNNCC 1 cut(s) 1627
BspMI ACCTGC 1 cut(s) 907
BspPI GGATC 4 cut(s) 395, 442, 458, 1149
BspT107I GGYRCC 1 cut(s) 1625
BspTI CTTAAG 1 cut(s) 1391
BspTNI GGTCTC 1 cut(s) 1272
BsrDI GCAATG 4 cut(s) 103, 701, 735, 1111
BsrFI RCCGGY 2 cut(s) 1176, 1313
BsrGI TGTACA 1 cut(s) 358
BsrI ACTGG 1 cut(s) 1336
BssAI RCCGGY 2 cut(s) 1176, 1313
BssECI CCNNGG 3 cut(s) 1114, 1283, 1602
BssSI CACGAG 1 cut(s) 1383
BssT1I CCWWGG 1 cut(s) 1114
Bst2BI CACGAG 1 cut(s) 1383
Bst2UI CCWGG 1 cut(s) 1284
Bst4CI ACNGT 5 cut(s) 91, 120, 949, 1075, 1266
Bst6I CTCTTC 2 cut(s) 676, 1434
BstAFI CTTAAG 1 cut(s) 1391
BstAUI TGTACA 1 cut(s) 358
BstBAI YACGTR 1 cut(s) 1373
BstC8I GCNNGC 3 cut(s) 261, 1046, 1102
BstDEI CTNAG 2 cut(s) 318, 984
BstEII GGTNACC 1 cut(s) 694
BstF5I GGATG 2 cut(s) 45, 216
BstH2I RGCGCY 1 cut(s) 627
BstHHI GCGC 3 cut(s) 626, 1209, 1329
BstMAI GTCTC 1 cut(s) 1272
BstMCI CGRYCG 2 cut(s) 493, 1177
BstMWI GCNNNNNNNGC 3 cut(s) 102, 1110, 1130
BstNI CCWGG 1 cut(s) 1284
BstNSI RCATGY 2 cut(s) 859, 1022
BstPI GGTNACC 1 cut(s) 694
BstSCI CCNGG 3 cut(s) 690, 1282, 1601
BstSFI CTRYAG 1 cut(s) 1583
BstSLI GKGCMC 1 cut(s) 738
BstV1I GCAGC 3 cut(s) 114, 1108, 1167
BstV2I GAAGAC 2 cut(s) 544, 782
BstX2I RGATCY 2 cut(s) 1141, 1354
BstYI RGATCY 2 cut(s) 1141, 1354
Bsu15I ATCGAT 1 cut(s) 188
BsuI GTATCC 1 cut(s) 307
BsuRI GGCC 4 cut(s) 923, 962, 1113, 1519
BsuTUI ATCGAT 1 cut(s) 188
BtsCI GGATG 2 cut(s) 45, 216
BtsI GCAGTG 2 cut(s) 522, 1638
BtsIMutI CAGTG 4 cut(s) 522, 719, 1329, 1638
BveI ACCTGC 1 cut(s) 907
Cac8I GCNNGC 3 cut(s) 261, 1046, 1102
CfoI GCGC 3 cut(s) 626, 1209, 1329
Cfr10I RCCGGY 2 cut(s) 1176, 1313
Cfr13I GGNCC 1 cut(s) 561
ClaI ATCGAT 1 cut(s) 188
Csp6I GTAC 3 cut(s) 359, 988, 1276
CviAII CATG 5 cut(s) 61, 617, 856, 1019, 1378
CviQI GTAC 3 cut(s) 359, 988, 1276
DdeI CTNAG 2 cut(s) 318, 984
EaeI YGGCCR 4 cut(s) 921, 960, 1111, 1517
Eam1104I CTCTTC 2 cut(s) 676, 1434
EarI CTCTTC 2 cut(s) 676, 1434
Eco130I CCWWGG 1 cut(s) 1114
Eco31I GGTCTC 1 cut(s) 1272
Eco32I GATATC 2 cut(s) 554, 1683
Eco47I GGWCC 1 cut(s) 561
Eco47III AGCGCT 1 cut(s) 625
Eco57I CTGAAG 3 cut(s) 29, 116, 213
Eco91I GGTNACC 1 cut(s) 694
EcoO109I RGGNCCY 1 cut(s) 561
EcoO65I GGTNACC 1 cut(s) 694
EcoRII CCWGG 1 cut(s) 1282
EcoRV GATATC 2 cut(s) 554, 1683
EcoT14I CCWWGG 1 cut(s) 1114
EcoT22I ATGCAT 1 cut(s) 841
ErhI CCWWGG 1 cut(s) 1114
FaeI CATG 5 cut(s) 64, 620, 859, 1022, 1381
FaqI GGGAC 1 cut(s) 1527
FatI CATG 5 cut(s) 60, 616, 855, 1018, 1377
FauI CCCGC 1 cut(s) 460
FauNDI CATATG 2 cut(s) 1126, 1701
FblI GTMKAC 1 cut(s) 1410
Fnu4HI GCNGC 5 cut(s) 128, 513, 1122, 1181, 1624
FokI GGATG 2 cut(s) 52, 203
Fsp4HI GCNGC 5 cut(s) 128, 513, 1122, 1181, 1624
FspBI CTAG 4 cut(s) 270, 1049, 1289, 1458
GlaI GCGC 3 cut(s) 625, 1208, 1328
GluI GCNGC 5 cut(s) 128, 513, 1122, 1181, 1624
HaeII RGCGCY 1 cut(s) 627
HaeIII GGCC 4 cut(s) 923, 962, 1113, 1519
HapII CCGG 6 cut(s) 37, 57, 692, 1177, 1314, 1602
HhaI GCGC 3 cut(s) 626, 1209, 1329
Hin1II CATG 5 cut(s) 64, 620, 859, 1022, 1381
Hin6I GCGC 3 cut(s) 624, 1207, 1327
HinP1I GCGC 3 cut(s) 624, 1207, 1327
HincII GTYRAC 1 cut(s) 973
HindII GTYRAC 1 cut(s) 973
HinfI GANTC 8 cut(s) 80, 164, 185, 265, 322, 338, 709, 1565
HpaII CCGG 6 cut(s) 37, 57, 692, 1177, 1314, 1602
HphI GGTGA 2 cut(s) 410, 1346
Hpy166II GTNNAC 6 cut(s) 717, 736, 769, 973, 1388, 1411
Hpy188III TCNNGA 6 cut(s) 47, 371, 755, 943, 1303, 1686
Hpy8I GTNNAC 6 cut(s) 717, 736, 769, 973, 1388, 1411
HpyAV CCTTC 2 cut(s) 58, 304
HpyCH4III ACNGT 5 cut(s) 91, 120, 949, 1075, 1266
HpyCH4IV ACGT 3 cut(s) 14, 1008, 1372
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 1110, 1130
HpyF3I CTNAG 2 cut(s) 318, 984
HpySE526I ACGT 3 cut(s) 14, 1008, 1372
Hsp92II CATG 5 cut(s) 64, 620, 859, 1022, 1381
HspAI GCGC 3 cut(s) 624, 1207, 1327
LmnI GCTCC 1 cut(s) 1118
Lsp1109I GCAGC 3 cut(s) 114, 1108, 1167
LweI GCATC 5 cut(s) 30, 437, 826, 987, 1227
MaeI CTAG 4 cut(s) 270, 1049, 1289, 1458
MaeII ACGT 3 cut(s) 14, 1008, 1372
MaeIII GTNAC 8 cut(s) 10, 192, 694, 929, 1021, 1030, 1135, 1373
MboII GAAGA 5 cut(s) 549, 693, 782, 1421, 1679
MfeI CAATTG 1 cut(s) 1241
MflI RGATCY 2 cut(s) 1141, 1354
MhlI GDGCHC 1 cut(s) 738
MlsI TGGCCA 3 cut(s) 923, 962, 1113
MluCI AATT 9 cut(s) 245, 507, 602, 672, 865, 1241, 1253, 1295, 1639
MluNI TGGCCA 3 cut(s) 923, 962, 1113
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 2 cut(s) 248, 1474
Mox20I TGGCCA 3 cut(s) 923, 962, 1113
Mph1103I ATGCAT 1 cut(s) 841
MroXI GAANNNNTTC 1 cut(s) 144
MscI TGGCCA 3 cut(s) 923, 962, 1113
MseI TTAA 4 cut(s) 33, 1148, 1392, 1638
MslI CAYNNNNRTG 1 cut(s) 1631
Msp20I TGGCCA 3 cut(s) 923, 962, 1113
MspCI CTTAAG 1 cut(s) 1391
MspI CCGG 6 cut(s) 37, 57, 692, 1177, 1314, 1602
MspR9I CCNGG 3 cut(s) 692, 1284, 1603
MunI CAATTG 1 cut(s) 1241
Mva1269I GAATGC 1 cut(s) 1594
MvaI CCWGG 1 cut(s) 1284
MwoI GCNNNNNNNGC 3 cut(s) 102, 1110, 1130
NciI CCSGG 2 cut(s) 692, 1603
NdeI CATATG 2 cut(s) 1126, 1701
NlaIII CATG 5 cut(s) 64, 620, 859, 1022, 1381
NlaIV GGNNCC 1 cut(s) 1627
NmeAIII GCCGAG 1 cut(s) 1599
NmuCI GTSAC 1 cut(s) 192
NsiI ATGCAT 1 cut(s) 841
NspI RCATGY 2 cut(s) 859, 1022
OliI CACNNNNGTG 1 cut(s) 1631
PaqCI CACCTGC 1 cut(s) 907
PciI ACATGT 1 cut(s) 855
PctI GAATGC 1 cut(s) 1594
PdmI GAANNNNTTC 1 cut(s) 144
PfeI GAWTC 7 cut(s) 164, 185, 265, 322, 338, 709, 1565
PflMI CCANNNNNTGG 1 cut(s) 1493
PkrI GCNGC 5 cut(s) 129, 514, 1123, 1182, 1625
Ple19I CGATCG 1 cut(s) 493
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
Ppu21I YACGTR 1 cut(s) 1373
PpuMI RGGWCCY 1 cut(s) 561
PscI ACATGT 1 cut(s) 855
Psp1406I AACGTT 1 cut(s) 1008
Psp5II RGGWCCY 1 cut(s) 561
Psp6I CCWGG 1 cut(s) 1282
PspEI GGTNACC 1 cut(s) 694
PspGI CCWGG 1 cut(s) 1282
PspN4I GGNNCC 1 cut(s) 1627
PspPI GGNCC 1 cut(s) 561
PspPPI RGGWCCY 1 cut(s) 561
PsrI GAACNNNNNNTAC 2 cut(s) 351, 383
PsuI RGATCY 2 cut(s) 1141, 1354
PvuI CGATCG 1 cut(s) 493
RsaI GTAC 3 cut(s) 360, 989, 1277
RsaNI GTAC 3 cut(s) 359, 988, 1276
RseI CAYNNNNRTG 1 cut(s) 1631
SaqAI TTAA 4 cut(s) 33, 1148, 1392, 1638
SatI GCNGC 5 cut(s) 128, 513, 1122, 1181, 1624
Sau96I GGNCC 1 cut(s) 561
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 3 cut(s) 692, 1284, 1603
SduI GDGCHC 1 cut(s) 738
SfaNI GCATC 5 cut(s) 30, 437, 826, 987, 1227
SfcI CTRYAG 1 cut(s) 1583
SinI GGWCC 1 cut(s) 561
SmiMI CAYNNNNRTG 1 cut(s) 1631
SmlI CTYRAG 1 cut(s) 1391
SmoI CTYRAG 1 cut(s) 1391
Sse9I AATT 9 cut(s) 245, 507, 602, 672, 865, 1241, 1253, 1295, 1639
SsiI CCGC 4 cut(s) 453, 512, 1623, 1629
SspI AATATT 1 cut(s) 238
SspMI CTAG 4 cut(s) 270, 1049, 1289, 1458
StyD4I CCNGG 3 cut(s) 690, 1282, 1601
StyI CCWWGG 1 cut(s) 1114
TaaI ACNGT 5 cut(s) 91, 120, 949, 1075, 1266
TaiI ACGT 3 cut(s) 17, 1011, 1375
TaqI TCGA 4 cut(s) 78, 188, 462, 1173
TasI AATT 9 cut(s) 245, 507, 602, 672, 865, 1241, 1253, 1295, 1639
TatI WGTACW 3 cut(s) 358, 987, 1275
TauI GCSGC 2 cut(s) 515, 1626
TfiI GAWTC 7 cut(s) 164, 185, 265, 322, 338, 709, 1565
Tru1I TTAA 4 cut(s) 33, 1148, 1392, 1638
Tru9I TTAA 4 cut(s) 33, 1148, 1392, 1638
TscAI CASTG 4 cut(s) 522, 719, 1336, 1638
TseFI GTSAC 1 cut(s) 192
TseI GCWGC 3 cut(s) 127, 1121, 1180
Tsp45I GTSAC 1 cut(s) 192
TspDTI ATGAA 7 cut(s) 77, 205, 314, 633, 876, 899, 1170
TspGWI ACGGA 4 cut(s) 209, 648, 658, 1154
TspRI CASTG 4 cut(s) 522, 719, 1336, 1638
Van91I CCANNNNNTGG 1 cut(s) 1493
Vha464I CTTAAG 1 cut(s) 1391
VneI GTGCAC 1 cut(s) 734
VpaK11BI GGWCC 1 cut(s) 561
XapI RAATTY 1 cut(s) 865
XceI RCATGY 2 cut(s) 859, 1022
XmiI GTMKAC 1 cut(s) 1410
XmnI GAANNNNTTC 1 cut(s) 144
XspI CTAG 4 cut(s) 270, 1049, 1289, 1458
Zsp2I ATGCAT 1 cut(s) 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.