Rorug06G0106200

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004658
Physical Location & Seq
Forward (+)
14282434 .. 14283774
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0106200.1

Sequence Viewer

Length: 1341 bp
ATGTCTTCACAAAAAGAAGATCGAAAACCAGAATCAGAGCTTCCCTTGAGAGAAATCCCTGGGGACGATGGCGTACCCTTCCTTGGGCCCATCAAAGATCGCCTAGACTATTTCTACAACCAAGGCAGAGACAACTTCTTCATCTCCCGAATCCTAAAGCACCAATCCACAGTCTTTAGGACAAACATGCCTCCTGGTCCCCTCATTGCTTCAAACCCCAACGTCGTCGTTCTCCTCGATGCCAAAAGCTTCCCCGTTCTCTTGGACACTTCCAAAGTCGAAAAACGAGACGTATTCACGGGAACTTACATGTCCTCGACCGCCTTCACCGGTGGCTACCGCATTTGCTCTTATCTCGACCCCTCCGAGCCGGACCATGCAGCTCTCAAGTACTGGTTCTTGTCCCAGCTCTCCACTCGCAAAGACAGAGTTATACCACTCTTTCGAAACCACTTGTCCGACCAGTTCCTCAAACTCGAGGACCAATTGTTCAAAAATGGTGAAGCAGACTTCAACAAACTAAACGACCAAATGTCCTTCAATTTCATCTTTGAGCTCTTTTGCGACAGACCACCATCGGACACAACTTTGAGGACCAAGGGTCCAATCTTTGTGTCCCTCTGGCTCTTGCCCCAGCTTTCTCCTCAGATAACACTAGGGTTGCCCAAGTTTCTAAACTTTGTCGAAGATTTCTTGCTGCACACTGTTCCATTTCCTGCTCTTCTTGTCAAACCATTCTACAACAAGCTTTACAATGTCTTTTGGGACTCCGCAACTTCGGCTTTGGACGAGGCCGAGAGGCTTGAGATTCCGAGAGACGAGGCTTGCCACAACCTGGTGTTCTTGGTATGCTTCAATGCTTATGGGGGCATGAAGCTTTTGTTCCCTGCTTTGTTGAAGTGGGTTGGAATAGCAGGACAGTACTTACACCGCCAACTCCGTAATGAAATCAGAACCGCTGTTAAAGAGGCCGGAGGAAAGGTAACCCTATCAGCATTGGAGAAGATGACCTTGACAAAATCGGTGGTTTATGAAGCCCTGAGGATTGAGCCGCCGGTGCCATTCCAGTACGGTAAGGCCAAGAGGGACATGATAGTCCACAGCCATGATGCTGCGTTTGAGATCAAGGAAGGGGAGATGTTGTTTGGTTACCAAACTATAGCAGAAAGGGATCCGAAGATTTTCGAGAATCCTAATGAGTTTGTGGGAGATAGGTTTGTTGGGGAGGGAGAGAAGCTGTTGGAGTACTTGTGGTGGTCCAACGGCCAGGAGACGAAGAGTCCGACGGTGGAGGATAAGCAGTGTGCAGGGAAAGATCTGGTTGTGCTCGATCATGAATAG

Protein Analysis

446

Amino Acids

51.07

Weight (kDa)

5.72

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 311 - 409 6.2e-14 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1094
AccB1I GGYRCC 1 cut(s) 1057
AccB7I CCANNNNNTGG 1 cut(s) 835
AciI CCGC 6 cut(s) 321, 340, 771, 931, 957, 1052
AclWI GGATC 2 cut(s) 1166, 1179
AcoI YGGCCR 1 cut(s) 1264
AfaI GTAC 5 cut(s) 75, 392, 923, 1070, 1247
AfiI CCNNNNNNNGG 3 cut(s) 83, 84, 835
AflIII ACRYGT 1 cut(s) 309
AgeI ACCGGT 1 cut(s) 329
AgsI TTSAA 6 cut(s) 213, 493, 514, 541, 856, 898
AhdI GACNNNNNGTC 3 cut(s) 532, 600, 1278
AjnI CCWGG 4 cut(s) 58, 193, 834, 1266
AloI GAACNNNNNNTCC 2 cut(s) 473, 505
AluBI AGCT 9 cut(s) 40, 249, 383, 409, 556, 637, 748, 877, 1237
AluI AGCT 9 cut(s) 40, 249, 383, 409, 556, 637, 748, 877, 1237
Alw21I GWGCWC 2 cut(s) 558, 1329
Alw26I GTCTC 4 cut(s) 123, 282, 810, 1265
AlwI GGATC 2 cut(s) 1166, 1179
Ama87I CYCGRG 1 cut(s) 476
AoxI GGCC 5 cut(s) 86, 792, 969, 1077, 1264
ApaI GGGCCC 1 cut(s) 90
ApeKI GCWGC 3 cut(s) 380, 697, 1112
AsiGI ACCGGT 1 cut(s) 329
Asp700I GAANNNNTTC 1 cut(s) 1181
AspS9I GGNCC 8 cut(s) 86, 87, 197, 373, 481, 594, 602, 1257
AsuHPI GGTGA 2 cut(s) 319, 512
AsuII TTCGAA 1 cut(s) 445
AvaI CYCGRG 1 cut(s) 476
AvaII GGWCC 6 cut(s) 197, 373, 481, 594, 602, 1257
AxyI CCTNAGG 1 cut(s) 1040
BaeGI GKGCMC 1 cut(s) 90
BamHI GGATCC 1 cut(s) 1171
BanI GGYRCC 1 cut(s) 1057
BanII GRGCYC 2 cut(s) 90, 558
Bbv12I GWGCWC 2 cut(s) 558, 1329
BbvI GCAGC 3 cut(s) 392, 684, 1099
BccI CCATC 3 cut(s) 62, 98, 583
BceAI ACGGC 1 cut(s) 1279
BciT130I CCWGG 4 cut(s) 60, 195, 836, 1268
BcoDI GTCTC 4 cut(s) 123, 282, 810, 1265
BfaI CTAG 2 cut(s) 104, 656
BfmI CTRYAG 1 cut(s) 1158
BglII AGATCT 1 cut(s) 1315
BisI GCNGC 4 cut(s) 381, 698, 1052, 1113
BlsI GCNGC 4 cut(s) 382, 699, 1053, 1114
BmcAI AGTACT 3 cut(s) 392, 923, 1247
Bme1390I CCNGG 4 cut(s) 60, 195, 836, 1268
Bme18I GGWCC 6 cut(s) 197, 373, 481, 594, 602, 1257
BmeRI GACNNNNNGTC 3 cut(s) 532, 600, 1278
BmeT110I CYCGRG 1 cut(s) 476
BmgT120I GGNCC 8 cut(s) 86, 87, 197, 373, 481, 594, 602, 1257
BmiI GGNNCC 5 cut(s) 88, 199, 603, 1059, 1173
BmrFI CCNGG 4 cut(s) 60, 195, 836, 1268
BmsI GCATC 2 cut(s) 229, 1099
Bpu14I TTCGAA 1 cut(s) 445
BpuEI CTTGAG 3 cut(s) 67, 371, 824
BsaJI CCNNGG 5 cut(s) 58, 59, 82, 121, 597
BsaWI WCCGGW 1 cut(s) 329
BsaXI ACNNNNNCTCC 6 cut(s) 966, 996, 1127, 1157, 1235, 1265
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 84, 835
Bse118I RCCGGY 2 cut(s) 329, 1054
Bse1I ACTGG 3 cut(s) 398, 463, 1066
Bse21I CCTNAGG 1 cut(s) 1040
Bse3DI GCAATG 1 cut(s) 204
BseBI CCWGG 4 cut(s) 60, 195, 836, 1268
BseDI CCNNGG 5 cut(s) 58, 59, 82, 121, 597
BseLI CCNNNNNNNGG 3 cut(s) 83, 84, 835
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 2 cut(s) 659, 1031
BseNI ACTGG 3 cut(s) 398, 463, 1066
BseRI GAGGAG 2 cut(s) 224, 633
BseSI GKGCMC 1 cut(s) 90
BseXI GCAGC 3 cut(s) 392, 684, 1099
BseYI CCCAGC 2 cut(s) 405, 633
BsgI GTGCAG 2 cut(s) 683, 1326
Bsh1285I CGRYCG 1 cut(s) 321
BshFI GGCC 5 cut(s) 88, 794, 971, 1079, 1266
BshNI GGYRCC 1 cut(s) 1057
BshTI ACCGGT 1 cut(s) 329
BsiEI CGRYCG 1 cut(s) 321
BsiHKAI GWGCWC 2 cut(s) 558, 1329
BsiHKCI CYCGRG 1 cut(s) 476
BsiSI CCGG 4 cut(s) 330, 371, 972, 1055
BslFI GGGAC 6 cut(s) 77, 183, 388, 601, 779, 1100
BslI CCNNNNNNNGG 3 cut(s) 83, 84, 835
BsmAI GTCTC 4 cut(s) 123, 282, 810, 1265
BsmBI CGTCTC 3 cut(s) 282, 810, 1265
BsmFI GGGAC 6 cut(s) 77, 183, 388, 601, 779, 1100
BsnI GGCC 5 cut(s) 88, 794, 971, 1079, 1266
BsoBI CYCGRG 1 cut(s) 476
Bsp119I TTCGAA 1 cut(s) 445
Bsp120I GGGCCC 1 cut(s) 86
Bsp1286I GDGCHC 3 cut(s) 90, 558, 1329
Bsp143I GATC 6 cut(s) 19, 97, 1122, 1171, 1315, 1330
BspACI CCGC 6 cut(s) 321, 340, 771, 931, 957, 1052
BspANI GGCC 5 cut(s) 88, 794, 971, 1079, 1266
BspCNI CTCAG 2 cut(s) 658, 1032
BspHI TCATGA 1 cut(s) 1333
BspLI GGNNCC 5 cut(s) 88, 199, 603, 1059, 1173
BspPI GGATC 2 cut(s) 1166, 1179
BspQI GCTCTTC 1 cut(s) 726
BspT104I TTCGAA 1 cut(s) 445
BspT107I GGYRCC 1 cut(s) 1057
BsrDI GCAATG 1 cut(s) 204
BsrFI RCCGGY 2 cut(s) 329, 1054
BsrI ACTGG 3 cut(s) 398, 463, 1066
BssAI RCCGGY 2 cut(s) 329, 1054
BssECI CCNNGG 5 cut(s) 58, 59, 82, 121, 597
BssMI GATC 6 cut(s) 19, 97, 1122, 1171, 1315, 1330
BssT1I CCWWGG 3 cut(s) 82, 121, 597
Bst2UI CCWGG 4 cut(s) 60, 195, 836, 1268
Bst4CI ACNGT 5 cut(s) 172, 706, 921, 1073, 1288
Bst6I CTCTTC 2 cut(s) 726, 1271
BstBI TTCGAA 1 cut(s) 445
BstC8I GCNNGC 1 cut(s) 826
BstDEI CTNAG 2 cut(s) 645, 1040
BstEII GGTNACC 2 cut(s) 982, 1148
BstKTI GATC 6 cut(s) 22, 100, 1125, 1174, 1318, 1333
BstMAI GTCTC 4 cut(s) 123, 282, 810, 1265
BstMBI GATC 6 cut(s) 19, 97, 1122, 1171, 1315, 1330
BstMCI CGRYCG 1 cut(s) 321
BstMWI GCNNNNNNNGC 2 cut(s) 779, 1057
BstNI CCWGG 4 cut(s) 60, 195, 836, 1268
BstNSI RCATGY 2 cut(s) 190, 313
BstPI GGTNACC 2 cut(s) 982, 1148
BstSCI CCNGG 4 cut(s) 58, 193, 834, 1266
BstSFI CTRYAG 1 cut(s) 1158
BstSLI GKGCMC 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 392, 684, 1099
BstX2I RGATCY 2 cut(s) 1171, 1315
BstYI RGATCY 2 cut(s) 1171, 1315
Bsu36I CCTNAGG 1 cut(s) 1040
BsuRI GGCC 5 cut(s) 88, 794, 971, 1079, 1266
BtsI GCAGTG 1 cut(s) 1307
BtsIMutI CAGTG 2 cut(s) 702, 1307
Cac8I GCNNGC 1 cut(s) 826
CciI TCATGA 1 cut(s) 1333
Cfr10I RCCGGY 2 cut(s) 329, 1054
Cfr13I GGNCC 8 cut(s) 86, 87, 197, 373, 481, 594, 602, 1257
CsiI ACCWGGT 1 cut(s) 834
Csp6I GTAC 5 cut(s) 74, 391, 922, 1069, 1246
CspAI ACCGGT 1 cut(s) 329
CspCI CAANNNNNGTGG 2 cut(s) 1005, 1040
CviAII CATG 7 cut(s) 187, 310, 377, 871, 1090, 1106, 1334
CviQI GTAC 5 cut(s) 74, 391, 922, 1069, 1246
DdeI CTNAG 2 cut(s) 645, 1040
DpnI GATC 6 cut(s) 21, 99, 1124, 1173, 1317, 1332
DpnII GATC 6 cut(s) 19, 97, 1122, 1171, 1315, 1330
DrdI GACNNNNNNGTC 1 cut(s) 1094
DriI GACNNNNNGTC 3 cut(s) 532, 600, 1278
DseDI GACNNNNNNGTC 1 cut(s) 1094
EaeI YGGCCR 1 cut(s) 1264
Eam1104I CTCTTC 2 cut(s) 726, 1271
Eam1105I GACNNNNNGTC 3 cut(s) 532, 600, 1278
EarI CTCTTC 2 cut(s) 726, 1271
Ecl136II GAGCTC 1 cut(s) 556
Eco130I CCWWGG 3 cut(s) 82, 121, 597
Eco24I GRGCYC 2 cut(s) 90, 558
Eco47I GGWCC 6 cut(s) 197, 373, 481, 594, 602, 1257
Eco53kI GAGCTC 1 cut(s) 556
Eco81I CCTNAGG 1 cut(s) 1040
Eco88I CYCGRG 1 cut(s) 476
Eco91I GGTNACC 2 cut(s) 982, 1148
EcoICRI GAGCTC 1 cut(s) 556
EcoO65I GGTNACC 2 cut(s) 982, 1148
EcoRII CCWGG 4 cut(s) 58, 193, 834, 1266
EcoT14I CCWWGG 3 cut(s) 82, 121, 597
EcoT38I GRGCYC 2 cut(s) 90, 558
ErhI CCWWGG 3 cut(s) 82, 121, 597
Esp3I CGTCTC 3 cut(s) 282, 810, 1265
FaeI CATG 7 cut(s) 190, 313, 380, 874, 1093, 1109, 1337
FalI AAGNNNNNCTT 2 cut(s) 995, 1027
FaqI GGGAC 6 cut(s) 77, 183, 388, 601, 779, 1100
FatI CATG 7 cut(s) 186, 309, 376, 870, 1089, 1105, 1333
Fnu4HI GCNGC 4 cut(s) 381, 698, 1052, 1113
FriOI GRGCYC 2 cut(s) 90, 558
Fsp4HI GCNGC 4 cut(s) 381, 698, 1052, 1113
FspBI CTAG 2 cut(s) 104, 656
GluI GCNGC 4 cut(s) 381, 698, 1052, 1113
GsaI CCCAGC 2 cut(s) 409, 637
HaeIII GGCC 5 cut(s) 88, 794, 971, 1079, 1266
HapII CCGG 4 cut(s) 330, 371, 972, 1055
Hin1II CATG 7 cut(s) 190, 313, 380, 874, 1093, 1109, 1337
HindIII AAGCTT 3 cut(s) 247, 746, 875
HinfI GANTC 6 cut(s) 32, 150, 767, 808, 1189, 1279
HpaII CCGG 4 cut(s) 330, 371, 972, 1055
HphI GGTGA 2 cut(s) 319, 512
Hpy166II GTNNAC 1 cut(s) 1099
Hpy188I TCNGA 9 cut(s) 37, 367, 460, 580, 648, 813, 953, 1176, 1284
Hpy188III TCNNGA 4 cut(s) 147, 356, 1186, 1334
Hpy8I GTNNAC 1 cut(s) 1099
Hpy99I CGWCG 3 cut(s) 227, 230, 1288
HpyAV CCTTC 4 cut(s) 88, 334, 547, 1124
HpyCH4III ACNGT 5 cut(s) 172, 706, 921, 1073, 1288
HpyCH4IV ACGT 2 cut(s) 222, 291
HpyCH4V TGCA 3 cut(s) 380, 700, 1307
HpyF10VI GCNNNNNNNGC 2 cut(s) 779, 1057
HpyF3I CTNAG 2 cut(s) 645, 1040
HpySE526I ACGT 2 cut(s) 222, 291
Hsp92II CATG 7 cut(s) 190, 313, 380, 874, 1093, 1109, 1337
Kzo9I GATC 6 cut(s) 19, 97, 1122, 1171, 1315, 1330
LguI GCTCTTC 1 cut(s) 726
Lsp1109I GCAGC 3 cut(s) 392, 684, 1099
LweI GCATC 2 cut(s) 229, 1099
MabI ACCWGGT 1 cut(s) 834
MaeI CTAG 2 cut(s) 104, 656
MaeII ACGT 2 cut(s) 222, 291
MaeIII GTNAC 2 cut(s) 982, 1148
MalI GATC 6 cut(s) 21, 99, 1124, 1173, 1317, 1332
MboI GATC 6 cut(s) 19, 97, 1122, 1171, 1315, 1330
MboII GAAGA 7 cut(s) 29, 130, 698, 713, 1015, 1189, 1288
MfeI CAATTG 1 cut(s) 485
MflI RGATCY 2 cut(s) 1171, 1315
MhlI GDGCHC 3 cut(s) 90, 558, 1329
MluCI AATT 2 cut(s) 485, 541
MlyI GAGTC 2 cut(s) 761, 1288
MmeI TCCRAC 5 cut(s) 483, 886, 1221, 1284, 1307
MroXI GAANNNNTTC 1 cut(s) 1181
MseI TTAA 1 cut(s) 963
MslI CAYNNNNRTG 1 cut(s) 1104
MspA1I CMGCKG 1 cut(s) 959
MspI CCGG 4 cut(s) 330, 371, 972, 1055
MspR9I CCNGG 4 cut(s) 60, 195, 836, 1268
MunI CAATTG 1 cut(s) 485
MvaI CCWGG 4 cut(s) 60, 195, 836, 1268
MwoI GCNNNNNNNGC 2 cut(s) 779, 1057
NdeII GATC 6 cut(s) 19, 97, 1122, 1171, 1315, 1330
NlaIII CATG 7 cut(s) 190, 313, 380, 874, 1093, 1109, 1337
NlaIV GGNNCC 5 cut(s) 88, 199, 603, 1059, 1173
NmeAIII GCCGAG 1 cut(s) 820
NspI RCATGY 2 cut(s) 190, 313
NspV TTCGAA 1 cut(s) 445
PaeR7I CTCGAG 1 cut(s) 476
PagI TCATGA 1 cut(s) 1333
PasI CCCWGGG 1 cut(s) 59
PciI ACATGT 1 cut(s) 309
PciSI GCTCTTC 1 cut(s) 726
PcsI WCGNNNNNNNCGW 3 cut(s) 234, 363, 1280
PdmI GAANNNNTTC 1 cut(s) 1181
PfeI GAWTC 4 cut(s) 32, 150, 808, 1189
PflMI CCANNNNNTGG 1 cut(s) 835
PinAI ACCGGT 1 cut(s) 329
PkrI GCNGC 4 cut(s) 382, 699, 1053, 1114
PleI GAGTC 2 cut(s) 761, 1287
PpsI GAGTC 2 cut(s) 761, 1287
PscI ACATGT 1 cut(s) 309
Psp124BI GAGCTC 1 cut(s) 558
Psp6I CCWGG 4 cut(s) 58, 193, 834, 1266
PspEI GGTNACC 2 cut(s) 982, 1148
PspFI CCCAGC 2 cut(s) 405, 633
PspGI CCWGG 4 cut(s) 58, 193, 834, 1266
PspN4I GGNNCC 5 cut(s) 88, 199, 603, 1059, 1173
PspOMI GGGCCC 1 cut(s) 86
PspPI GGNCC 8 cut(s) 86, 87, 197, 373, 481, 594, 602, 1257
PspXI VCTCGAGB 1 cut(s) 476
PsuI RGATCY 2 cut(s) 1171, 1315
RsaI GTAC 5 cut(s) 75, 392, 923, 1070, 1247
RsaNI GTAC 5 cut(s) 74, 391, 922, 1069, 1246
RseI CAYNNNNRTG 1 cut(s) 1104
SacI GAGCTC 1 cut(s) 558
SapI GCTCTTC 1 cut(s) 726
SaqAI TTAA 1 cut(s) 963
SatI GCNGC 4 cut(s) 381, 698, 1052, 1113
Sau3AI GATC 6 cut(s) 19, 97, 1122, 1171, 1315, 1330
Sau96I GGNCC 8 cut(s) 86, 87, 197, 373, 481, 594, 602, 1257
ScaI AGTACT 3 cut(s) 392, 923, 1247
SchI GAGTC 2 cut(s) 761, 1288
ScrFI CCNGG 4 cut(s) 60, 195, 836, 1268
SduI GDGCHC 3 cut(s) 90, 558, 1329
SexAI ACCWGGT 1 cut(s) 834
SfaNI GCATC 2 cut(s) 229, 1099
SfcI CTRYAG 1 cut(s) 1158
Sfr274I CTCGAG 1 cut(s) 476
SfuI TTCGAA 1 cut(s) 445
SgrAI CRCCGGYG 2 cut(s) 329, 1054
SinI GGWCC 6 cut(s) 197, 373, 481, 594, 602, 1257
SlaI CTCGAG 1 cut(s) 476
SmiMI CAYNNNNRTG 1 cut(s) 1104
SmlI CTYRAG 4 cut(s) 46, 386, 476, 803
SmoI CTYRAG 4 cut(s) 46, 386, 476, 803
Sse9I AATT 2 cut(s) 485, 541
SsiI CCGC 6 cut(s) 321, 340, 771, 931, 957, 1052
SspMI CTAG 2 cut(s) 104, 656
SstI GAGCTC 1 cut(s) 558
StyD4I CCNGG 4 cut(s) 58, 193, 834, 1266
StyI CCWWGG 3 cut(s) 82, 121, 597
TaaI ACNGT 5 cut(s) 172, 706, 921, 1073, 1288
TaiI ACGT 2 cut(s) 225, 294
TasI AATT 2 cut(s) 485, 541
TatI WGTACW 3 cut(s) 390, 921, 1245
TauI GCSGC 1 cut(s) 1054
TfiI GAWTC 4 cut(s) 32, 150, 808, 1189
Tru1I TTAA 1 cut(s) 963
Tru9I TTAA 1 cut(s) 963
TscAI CASTG 2 cut(s) 709, 1307
TseI GCWGC 3 cut(s) 380, 697, 1112
TspDTI ATGAA 5 cut(s) 130, 535, 887, 960, 1047
TspGWI ACGGA 1 cut(s) 929
TspRI CASTG 2 cut(s) 709, 1307
Van91I CCANNNNNTGG 1 cut(s) 835
VpaK11BI GGWCC 6 cut(s) 197, 373, 481, 594, 602, 1257
XceI RCATGY 2 cut(s) 190, 313
XhoI CTCGAG 1 cut(s) 476
XmnI GAANNNNTTC 1 cut(s) 1181
XspI CTAG 2 cut(s) 104, 656
ZrmI AGTACT 3 cut(s) 392, 923, 1247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.