Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004658

Physical Location & Seq

Genomic Coordinates Forward (+)

2891390 .. 2905690

Gene Structure 14301 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1016500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1704 bp

ATGGTGCTGGTCACGGCTGTACGGGATTACTTAAGCCGGATGTTGCAGGACATCTCAGGCATGAAGGTTCTCATCCTCGATTCCCACACGGTTAGTATAGTGAGCGTGGCGTATTCGCAATCCGAGCTTCTGCAGAAAGAAGTGTTTTTGGTGGAATTGGTAGACTCCATTTCCAAGTCCAGAGAATCCATGTCGCATCTCAAAGCCGTTTACTTTCTCGCACCCACTTCAGAGAACATACAGTTTTTGCGCCGGCACTTGGTGTCTCCTCGATTCGGGGAGTATCACCTCTTTTTCTCCAACATATTGAAGGATACCCAGATTCATATTTTAGCTGATTCAGATGAGCATGAAGTTGTGCAGCAGCTTCAGGAGTTCTATGCAGACTTTGTTGCAACTGATCCTTACCATTTTACTTTGAATGTGCCATCAAATCACATGTATATGCTCCCAGCAGTTGTGGATCATTCAAATTTACAGTGCTTCTGTGATCGAGTAGTTGATGGAATTGCAGCTGTTTTTTTGGCATTAAAACGAAGACCAGTTATTCGTTATCAGAGAACATCTGATATTGCTAAAAGGATTGCGCAGGAAACAGCAAAGCTGATGTACCAGCAGGAAAGTGGACTTTTTGATTTCAGACGAACAGAAATTTCTCCGTTGTTGCTGGTAATTGATAGGAGGGATGATCCTGTGACCCCGTTGCTGAATCAGTGGACCTATCAGGCCATGGTTCACGAATTGATTGGTATTCAAGACAACAAAGTCGACTTAAGAAACATTGGAAAATTTCCAAAGGATCAAGAGGAGGTTGTGCTGTCATCAGAGCAAGATGCTTTTTTCAAAAAGAACATGTATGAGAACTTTGGTGATATTGGGATGAACATCAAGCAGTTGGTTGATGAATTTCAGCAAATTTCAAAATGTAACCAGAACATTCAGACGATAGAGGACATGGCCAAGTTTGTTGACAATTACCCTGAGTACAGAAAGATGCATGGAAATGTTTCAAAGCATGTGGCACTGGTAACAGAAATGAGCAAGCTAGTTGAAGAGCGAAAACTTATGGTAGTGTCACAGACAGAGCAGGAGTTGGCTTGCAACGGTGGGCAGGTGGCAGCTTTTGAGGCAGTGACAAATCTATTAAACAATGAAAGCGTATCTGATATTGACCGCCTACGACTTGTCATGCTGTATGCTTTACGCTATGAGAAGGAGAGTCCTGTGCAACTAATGCAGTTATTTAACAAACTGTCATCCAAGTCTCCCAAGTACAAGCCTGGGCTTGTCCAGTTTCTCTTGAAGCAAGCGGGTGTGGATAAGCGAACCGGGGATCTTTATGGAAATCGAGATTTCTTAAACATCGCGCGTAACATGGCTCGTGGGCTGAAGGGGGTTGAGAATGTGTACACACAACACCAGCCTCTTCTCTTCCAAACTATGGAAAGCATCACCAAGGGACGGCTGAGAGATGTGGATTACCCATTTGTGGGAAATCATTTCCAGCAGGGCAGGCCTCAAGAAGTGGTCATTTTCATTGTTGGGGGGACAACGTACGAGGAGTCTCGTTGTGTAGCTTTACAAAATGCTATGAATACTGGAATTCGTTTTATACTTGGTGGTTCTGTGATTCTCAATTCTAAAAGGTTCTTGAAGGACTTGGAAGAAGCTCAGAGAATAGCTCGTACTAGCAATGCCATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000325 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0009705 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0031090 GO:0031984 GO:0032588 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0098588 GO:0098791 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04138 ko04144 map04138 map04144

Pfam Domains

Protein Families

Sec1

Protein Analysis

568

Amino Acids

65.05

Weight (kDa)

6.42

Isoelectric Point (pI)

51.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sec1	PF00995	22 - 553	2.6e-124	Sec1 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004658)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G77140
fragaria_vesca	FvH4_2g14590 FvH4_2g14590 FvH4_2g14590 FvH4_2g14590 FvH4_2g14590 FvH4_2g14590 FvH4_2g14590
malus_domestica	MD05G1016500.v1.1 MD10G1016000.v1.1 MD10G1016100.v1.1
prunus_persica	Prupe.8G019800_v2.0.a1 Prupe.8G019800_v2.0.a1
pyrus_communis	pycom05g00910 pycom10g01040 pycom10g01050
rosa_chinensis	RchiOBHm_Chr6g0277061
rosa_laevigata	RLG00000013340
rosa_multiflora	Rmu_sc0000206.1_g000006
rosa_roxburghii	Rroxscaffold_7G00191710
rosa_rugosa	Rorug06G0106100 Rorug06G0106200 Rorug06G0106300 Rorug06G0106300 Rorug06G0106300 Rorug06G0106300
rosa_samantha	Rh6AG216000 Rh6BG220600 Rh6CG223400 Rh6DG213000
rosa_wichuraiana	Rw6G018850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1102
AasI	GACNNNNNNGTC	1 cut(s)	764
Acc16I	TGCGCA	1 cut(s)	588
Acc36I	ACCTGC	1 cut(s)	1102
AccB7I	CCANNNNNTGG	1 cut(s)	1441
AccI	GTMKAC	2 cut(s)	162, 768
AccII	CGCG	2 cut(s)	1367, 1369
AciI	CCGC	2 cut(s)	1174, 1310
AclWI	GGATC	5 cut(s)	395, 471, 683, 807, 1341
AcoI	YGGCCR	1 cut(s)	957
AcsI	RAATTY	6 cut(s)	472, 651, 788, 905, 915, 1602
AcuI	CTGAAG	3 cut(s)	213, 353, 1409
AdeI	CACNNNGTG	1 cut(s)	262
AfaI	GTAC	7 cut(s)	21, 611, 986, 1274, 1409, 1556, 1687
AfiI	CCNNNNNNNGG	6 cut(s)	259, 275, 1441, 1461, 1489, 1490
AflII	CTTAAG	2 cut(s)	31, 772
AflIII	ACRYGT	2 cut(s)	438, 852
AjnI	CCWGG	1 cut(s)	1279
Alw26I	GTCTC	3 cut(s)	270, 1269, 1569
AlwI	GGATC	5 cut(s)	395, 471, 683, 807, 1341
AoxI	GGCC	3 cut(s)	726, 957, 1514
ApeKI	GCWGC	4 cut(s)	361, 364, 512, 1118
ApoI	RAATTY	6 cut(s)	472, 651, 788, 905, 915, 1602
ArsI	GACNNNNNNTTYG	2 cut(s)	531, 563
Asp700I	GAANNNNTTC	1 cut(s)	1006
AspLEI	GCGC	3 cut(s)	252, 589, 1369
AspS9I	GGNCC	1 cut(s)	717
AsuC2I	CCSGG	1 cut(s)	1330
AsuHPI	GGTGA	3 cut(s)	278, 881, 1444
AvaII	GGWCC	1 cut(s)	717
BalI	TGGCCA	1 cut(s)	959
BauI	CACGAG	1 cut(s)	1380
BbsI	GAAGAC	1 cut(s)	544
BbvI	GCAGC	4 cut(s)	373, 376, 524, 1130
BccI	CCATC	2 cut(s)	436, 497
BceAI	ACGGC	3 cut(s)	30, 191, 1478
BciT130I	CCWGG	1 cut(s)	1281
BciVI	GTATCC	1 cut(s)	307
BcnI	CCSGG	1 cut(s)	1330
BcoDI	GTCTC	3 cut(s)	270, 1269, 1569
BfaI	CTAG	2 cut(s)	1046, 1689
BfmI	CTRYAG	1 cut(s)	131
BfrI	CTTAAG	2 cut(s)	31, 772
BfuAI	ACCTGC	1 cut(s)	1102
BfuI	GTATCC	1 cut(s)	307
BisI	GCNGC	4 cut(s)	362, 365, 513, 1119
BlsI	GCNGC	4 cut(s)	363, 366, 514, 1120
Bme1390I	CCNGG	2 cut(s)	1281, 1330
Bme18I	GGWCC	1 cut(s)	717
BmgT120I	GGNCC	1 cut(s)	717
BmrFI	CCNGG	2 cut(s)	1281, 1330
BmsI	GCATC	4 cut(s)	205, 823, 984, 1458
BpiI	GAAGAC	1 cut(s)	544
BplI	GAGNNNNNCTC	2 cut(s)	1666, 1698
BpuEI	CTTGAG	1 cut(s)	1503
BpuMI	CCSGG	1 cut(s)	1330
BsaBI	GATNNNNATC	1 cut(s)	884
BsaJI	CCNNGG	4 cut(s)	729, 1280, 1329, 1455
Bsc4I	CCNNNNNNNGG	6 cut(s)	259, 275, 1441, 1461, 1489, 1490
Bse118I	RCCGGY	1 cut(s)	252
Bse1I	ACTGG	4 cut(s)	542, 1029, 1291, 1603
Bse3DI	GCAATG	1 cut(s)	1699
Bse8I	GATNNNNATC	1 cut(s)	884
BseBI	CCWGG	1 cut(s)	1281
BseDI	CCNNGG	4 cut(s)	729, 1280, 1329, 1455
BseGI	GGATG	5 cut(s)	45, 72, 691, 885, 1256
BseJI	GATNNNNATC	1 cut(s)	884
BseLI	CCNNNNNNNGG	6 cut(s)	259, 275, 1441, 1461, 1489, 1490
BseMI	GCAATG	1 cut(s)	1699
BseMII	CTCAG	4 cut(s)	69, 972, 1457, 1685
BseNI	ACTGG	4 cut(s)	542, 1029, 1291, 1603
BseRI	GAGGAG	3 cut(s)	258, 821, 1574
BseXI	GCAGC	4 cut(s)	373, 376, 524, 1130
BseYI	CCCAGC	1 cut(s)	451
BsgI	GTGCAG	1 cut(s)	380
Bsh1236I	CGCG	2 cut(s)	1367, 1369
BshFI	GGCC	3 cut(s)	728, 959, 1516
BsiSI	CCGG	3 cut(s)	37, 253, 1329
BsiWI	CGTACG	1 cut(s)	1554
BslFI	GGGAC	2 cut(s)	1473, 1561
BslI	CCNNNNNNNGG	6 cut(s)	259, 275, 1441, 1461, 1489, 1490
BsmAI	GTCTC	3 cut(s)	270, 1269, 1569
BsmFI	GGGAC	2 cut(s)	1473, 1561
BsnI	GGCC	3 cut(s)	728, 959, 1516
Bsp1407I	TGTACA	1 cut(s)	1407
Bsp143I	GATC	6 cut(s)	400, 463, 490, 688, 799, 1333
Bsp19I	CCATGG	1 cut(s)	729
BspACI	CCGC	2 cut(s)	1174, 1310
BspANI	GGCC	3 cut(s)	728, 959, 1516
BspCNI	CTCAG	4 cut(s)	68, 973, 1458, 1684
BspFNI	CGCG	2 cut(s)	1367, 1369
BspMAI	CTGCAG	1 cut(s)	135
BspMI	ACCTGC	1 cut(s)	1102
BspPI	GGATC	5 cut(s)	395, 471, 683, 807, 1341
BspQI	GCTCTTC	1 cut(s)	1047
BspTI	CTTAAG	2 cut(s)	31, 772
BsrDI	GCAATG	1 cut(s)	1699
BsrFI	RCCGGY	1 cut(s)	252
BsrGI	TGTACA	1 cut(s)	1407
BsrI	ACTGG	4 cut(s)	542, 1029, 1291, 1603
BssAI	RCCGGY	1 cut(s)	252
BssECI	CCNNGG	4 cut(s)	729, 1280, 1329, 1455
BssMI	GATC	6 cut(s)	400, 463, 490, 688, 799, 1333
BssSI	CACGAG	1 cut(s)	1380
BssT1I	CCWWGG	2 cut(s)	729, 1455
Bst2BI	CACGAG	1 cut(s)	1380
Bst2UI	CCWGG	1 cut(s)	1281
Bst4CI	ACNGT	5 cut(s)	91, 243, 480, 1106, 1254
Bst6I	CTCTTC	3 cut(s)	1047, 1431, 1436
BstAFI	CTTAAG	2 cut(s)	31, 772
BstAPI	GCANNNNNTGC	1 cut(s)	1234
BstAUI	TGTACA	1 cut(s)	1407
BstC8I	GCNNGC	5 cut(s)	254, 1043, 1099, 1308, 1514
BstDEI	CTNAG	4 cut(s)	55, 981, 1466, 1671
BstDSI	CCRYGG	1 cut(s)	729
BstF5I	GGATG	5 cut(s)	45, 72, 691, 885, 1256
BstFNI	CGCG	2 cut(s)	1367, 1369
BstHHI	GCGC	3 cut(s)	252, 589, 1369
BstKTI	GATC	6 cut(s)	403, 466, 493, 691, 802, 1336
BstMAI	GTCTC	3 cut(s)	270, 1269, 1569
BstMBI	GATC	6 cut(s)	400, 463, 490, 688, 799, 1333
BstMWI	GCNNNNNNNGC	4 cut(s)	124, 1127, 1234, 1513
BstNI	CCWGG	1 cut(s)	1281
BstNSI	RCATGY	3 cut(s)	442, 856, 1019
BstSCI	CCNGG	2 cut(s)	1279, 1328
BstSFI	CTRYAG	1 cut(s)	131
BstUI	CGCG	2 cut(s)	1367, 1369
BstV1I	GCAGC	4 cut(s)	373, 376, 524, 1130
BstV2I	GAAGAC	1 cut(s)	544
BstX2I	RGATCY	1 cut(s)	1333
BstYI	RGATCY	1 cut(s)	1333
BsuI	GTATCC	1 cut(s)	307
BsuRI	GGCC	3 cut(s)	728, 959, 1516
BtgI	CCRYGG	1 cut(s)	729
BtgZI	GCGATG	1 cut(s)	1348
BtsCI	GGATG	5 cut(s)	45, 72, 691, 885, 1256
BtsI	GCAGTG	1 cut(s)	1137
BtsIMutI	CAGTG	4 cut(s)	485, 719, 1022, 1137
BveI	ACCTGC	1 cut(s)	1102
Cac8I	GCNNGC	5 cut(s)	254, 1043, 1099, 1308, 1514
CfoI	GCGC	3 cut(s)	252, 589, 1369
Cfr10I	RCCGGY	1 cut(s)	252
Cfr13I	GGNCC	1 cut(s)	717
Csp6I	GTAC	7 cut(s)	20, 610, 985, 1273, 1408, 1555, 1686
CspCI	CAANNNNNGTGG	2 cut(s)	999, 1034
CviQI	GTAC	7 cut(s)	20, 610, 985, 1273, 1408, 1555, 1686
DdeI	CTNAG	4 cut(s)	55, 981, 1466, 1671
DpnI	GATC	6 cut(s)	402, 465, 492, 690, 801, 1335
DpnII	GATC	6 cut(s)	400, 463, 490, 688, 799, 1333
DraIII	CACNNNGTG	1 cut(s)	262
DrdI	GACNNNNNNGTC	1 cut(s)	764
DseDI	GACNNNNNNGTC	1 cut(s)	764
EaeI	YGGCCR	1 cut(s)	957
Eam1104I	CTCTTC	3 cut(s)	1047, 1431, 1436
EarI	CTCTTC	3 cut(s)	1047, 1431, 1436
Eco130I	CCWWGG	2 cut(s)	729, 1455
Eco147I	AGGCCT	1 cut(s)	1516
Eco47I	GGWCC	1 cut(s)	717
Eco57I	CTGAAG	3 cut(s)	213, 353, 1409
EcoRI	GAATTC	1 cut(s)	1602
EcoRII	CCWGG	1 cut(s)	1279
EcoT14I	CCWWGG	2 cut(s)	729, 1455
EcoT22I	ATGCAT	1 cut(s)	999
ErhI	CCWWGG	2 cut(s)	729, 1455
FaqI	GGGAC	2 cut(s)	1473, 1561
FauI	CCCGC	1 cut(s)	1303
FblI	GTMKAC	2 cut(s)	162, 768
Fnu4HI	GCNGC	4 cut(s)	362, 365, 513, 1119
FokI	GGATG	5 cut(s)	52, 59, 698, 892, 1243
Fsp4HI	GCNGC	4 cut(s)	362, 365, 513, 1119
FspBI	CTAG	2 cut(s)	1046, 1689
FspI	TGCGCA	1 cut(s)	588
GlaI	GCGC	3 cut(s)	251, 588, 1368
GluI	GCNGC	4 cut(s)	362, 365, 513, 1119
GsaI	CCCAGC	1 cut(s)	455
HaeIII	GGCC	3 cut(s)	728, 959, 1516
HapII	CCGG	3 cut(s)	37, 253, 1329
HhaI	GCGC	3 cut(s)	252, 589, 1369
Hin6I	GCGC	3 cut(s)	250, 587, 1367
HinP1I	GCGC	3 cut(s)	250, 587, 1367
HincII	GTYRAC	2 cut(s)	769, 970
HindII	GTYRAC	2 cut(s)	769, 970
HpaII	CCGG	3 cut(s)	37, 253, 1329
HphI	GGTGA	3 cut(s)	278, 881, 1444
Hpy166II	GTNNAC	9 cut(s)	163, 211, 626, 717, 736, 769, 970, 1408, 1410
Hpy188III	TCNNGA	9 cut(s)	180, 371, 737, 755, 803, 1300, 1349, 1520, 1651
Hpy8I	GTNNAC	9 cut(s)	163, 211, 626, 717, 736, 769, 970, 1408, 1410
HpyAV	CCTTC	5 cut(s)	58, 304, 1207, 1384, 1648
HpyCH4III	ACNGT	5 cut(s)	91, 243, 480, 1106, 1254
HpyCH4IV	ACGT	1 cut(s)	1553
HpyF10VI	GCNNNNNNNGC	4 cut(s)	124, 1127, 1234, 1513
HpyF3I	CTNAG	4 cut(s)	55, 981, 1466, 1671
HpySE526I	ACGT	1 cut(s)	1553
HspAI	GCGC	3 cut(s)	250, 587, 1367
KroI	GCCGGC	1 cut(s)	252
KroNI	GCCGGC	1 cut(s)	254
Kzo9I	GATC	6 cut(s)	400, 463, 490, 688, 799, 1333
LguI	GCTCTTC	1 cut(s)	1047
LmnI	GCTCC	1 cut(s)	453
Lsp1109I	GCAGC	4 cut(s)	373, 376, 524, 1130
LweI	GCATC	4 cut(s)	205, 823, 984, 1458
MaeI	CTAG	2 cut(s)	1046, 1689
MaeII	ACGT	1 cut(s)	1553
MaeIII	GTNAC	7 cut(s)	10, 694, 926, 1027, 1074, 1132, 1370
MalI	GATC	6 cut(s)	402, 465, 492, 690, 801, 1335
MboI	GATC	6 cut(s)	400, 463, 490, 688, 799, 1333
MboII	GAAGA	5 cut(s)	549, 1064, 1418, 1423, 1676
MflI	RGATCY	1 cut(s)	1333
MlsI	TGGCCA	1 cut(s)	959
MluNI	TGGCCA	1 cut(s)	959
MlyI	GAGTC	3 cut(s)	158, 1228, 1571
MmeI	TCCRAC	1 cut(s)	324
Mox20I	TGGCCA	1 cut(s)	959
Mph1103I	ATGCAT	1 cut(s)	999
MroNI	GCCGGC	1 cut(s)	252
MroXI	GAANNNNTTC	1 cut(s)	1006
MscI	TGGCCA	1 cut(s)	959
MseI	TTAA	6 cut(s)	32, 530, 773, 1145, 1245, 1358
MslI	CAYNNNNRTG	2 cut(s)	443, 1002
Msp20I	TGGCCA	1 cut(s)	959
MspA1I	CMGCKG	1 cut(s)	515
MspCI	CTTAAG	2 cut(s)	31, 772
MspI	CCGG	3 cut(s)	37, 253, 1329
MspR9I	CCNGG	2 cut(s)	1281, 1330
MvaI	CCWGG	1 cut(s)	1281
MvnI	CGCG	2 cut(s)	1367, 1369
MwoI	GCNNNNNNNGC	4 cut(s)	124, 1127, 1234, 1513
NaeI	GCCGGC	1 cut(s)	254
NciI	CCSGG	1 cut(s)	1330
NcoI	CCATGG	1 cut(s)	729
NdeII	GATC	6 cut(s)	400, 463, 490, 688, 799, 1333
NgoMIV	GCCGGC	1 cut(s)	252
NmuCI	GTSAC	4 cut(s)	10, 694, 1074, 1132
NsbI	TGCGCA	1 cut(s)	588
NsiI	ATGCAT	1 cut(s)	999
NspI	RCATGY	3 cut(s)	442, 856, 1019
PaqCI	CACCTGC	1 cut(s)	1102
PceI	AGGCCT	1 cut(s)	1516
PciI	ACATGT	2 cut(s)	438, 852
PciSI	GCTCTTC	1 cut(s)	1047
PdiI	GCCGGC	1 cut(s)	254
PdmI	GAANNNNTTC	1 cut(s)	1006
PfeI	GAWTC	7 cut(s)	80, 185, 273, 322, 338, 709, 1630
Pfl23II	CGTACG	1 cut(s)	1554
PflMI	CCANNNNNTGG	1 cut(s)	1441
PkrI	GCNGC	4 cut(s)	363, 366, 514, 1120
PleI	GAGTC	3 cut(s)	158, 1227, 1570
PpsI	GAGTC	3 cut(s)	158, 1227, 1570
PscI	ACATGT	2 cut(s)	438, 852
Psp6I	CCWGG	1 cut(s)	1279
PspFI	CCCAGC	1 cut(s)	451
PspGI	CCWGG	1 cut(s)	1279
PspLI	CGTACG	1 cut(s)	1554
PspPI	GGNCC	1 cut(s)	717
PstI	CTGCAG	1 cut(s)	135
PsuI	RGATCY	1 cut(s)	1333
PvuII	CAGCTG	1 cut(s)	515
RsaI	GTAC	7 cut(s)	21, 611, 986, 1274, 1409, 1556, 1687
RsaNI	GTAC	7 cut(s)	20, 610, 985, 1273, 1408, 1555, 1686
RseI	CAYNNNNRTG	2 cut(s)	443, 1002
SalI	GTCGAC	1 cut(s)	767
SapI	GCTCTTC	1 cut(s)	1047
SaqAI	TTAA	6 cut(s)	32, 530, 773, 1145, 1245, 1358
SatI	GCNGC	4 cut(s)	362, 365, 513, 1119
Sau3AI	GATC	6 cut(s)	400, 463, 490, 688, 799, 1333
Sau96I	GGNCC	1 cut(s)	717
SchI	GAGTC	3 cut(s)	158, 1228, 1571
ScrFI	CCNGG	2 cut(s)	1281, 1330
SfaNI	GCATC	4 cut(s)	205, 823, 984, 1458
SfcI	CTRYAG	1 cut(s)	131
SinI	GGWCC	1 cut(s)	717
SmiMI	CAYNNNNRTG	2 cut(s)	443, 1002
SmlI	CTYRAG	3 cut(s)	31, 772, 1518
SmoI	CTYRAG	3 cut(s)	31, 772, 1518
SseBI	AGGCCT	1 cut(s)	1516
SsiI	CCGC	2 cut(s)	1174, 1310
SspMI	CTAG	2 cut(s)	1046, 1689
StuI	AGGCCT	1 cut(s)	1516
StyD4I	CCNGG	2 cut(s)	1279, 1328
StyI	CCWWGG	2 cut(s)	729, 1455
TaaI	ACNGT	5 cut(s)	91, 243, 480, 1106, 1254
TaiI	ACGT	1 cut(s)	1556
TaqI	TCGA	5 cut(s)	78, 271, 493, 768, 1348
TatI	WGTACW	3 cut(s)	984, 1272, 1407
TfiI	GAWTC	7 cut(s)	80, 185, 273, 322, 338, 709, 1630
Tru1I	TTAA	6 cut(s)	32, 530, 773, 1145, 1245, 1358
Tru9I	TTAA	6 cut(s)	32, 530, 773, 1145, 1245, 1358
TscAI	CASTG	4 cut(s)	485, 719, 1029, 1137
TseFI	GTSAC	4 cut(s)	10, 694, 1074, 1132
TseI	GCWGC	4 cut(s)	361, 364, 512, 1118
Tsp45I	GTSAC	4 cut(s)	10, 694, 1074, 1132
TspDTI	ATGAA	8 cut(s)	77, 314, 366, 896, 918, 1167, 1525, 1607
TspGWI	ACGGA	1 cut(s)	648
TspRI	CASTG	4 cut(s)	485, 719, 1029, 1137
Van91I	CCANNNNNTGG	1 cut(s)	1441
Vha464I	CTTAAG	2 cut(s)	31, 772
VpaK11BI	GGWCC	1 cut(s)	717
XapI	RAATTY	6 cut(s)	472, 651, 788, 905, 915, 1602
XceI	RCATGY	3 cut(s)	442, 856, 1019
XcmI	CCANNNNNNNNNTGG	1 cut(s)	620
XmiI	GTMKAC	2 cut(s)	162, 768
XmnI	GAANNNNTTC	1 cut(s)	1006
XspI	CTAG	2 cut(s)	1046, 1689
Zsp2I	ATGCAT	1 cut(s)	999

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.