AT1G79985

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010851
Physical Location & Seq
Forward (+)
30084114 .. 30085601
1488 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G79985.2

Sequence Viewer

Length: 459 bp
ATGTTCTATGGCACAGCTGTATGGGACCCTTGGCTTATCGTTGGCCAGATTATTTGCCTCCAATGCTCTTACTATCTCACTCTTGGACTCTTCACCATGGTCTTTCTTGGCCTTCGTGTTCCTCGCCTTAGTCTTGTCTACTTCTTCGATTACGCTACTCTCACTACTTCCACCTTCACCGGTTGGTCTGTTATTGCCTCATTCCTCTTCTCTTCACTCGCTGGGGCTGTGTACATGATATTTTTGGTGGAGCGAGCACGGAAATGCTTAGATTTCTCTGCAACTCTCTACATCATACATCTCTTCTTTTGCATCATGTATGGAGGATGGCCTTCCTCTATGGCGTGGTGGGTTGTTAATGGAACCGGACTCGCTGTTATGGCTTTGCTAGCTGAGTACTTGTGCATTAAACGCGAACAGCGAGAGATCCCTATGGATCGCTTCCACTCAAGGGTTTGA

Protein Analysis

152

Amino Acids

17.5

Weight (kDa)

8.3

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SYS1 PF09801 4 - 145 4.2e-48 Integral membrane protein S linking to the trans Golgi network
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010851)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79985 AT1G79985 AT1G79985
fragaria_vesca FvH4_6g31060 FvH4_6g49470
malus_domestica MD09G1213900.v1.1 MD17G1196000.v1.1
prunus_persica Prupe.3G071900_v2.0.a1 Prupe.3G071900_v2.0.a1
pyrus_communis pycom09g13080
rosa_chinensis RchiOBHm_Chr2g0137751
rosa_laevigata RLG00000019673
rosa_multiflora Rmu_sc0008270.1_g000012
rosa_roxburghii Rroxscaffold_2G00106870
rosa_rugosa Rorug02G0344700.1 Rorug02G0344800 Rorug02G0344900
rosa_samantha Rh2BG400000 Rh2DG414800
rosa_wichuraiana Rw2G032150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 138
AccII CGCG 1 cut(s) 414
AclWI GGATC 2 cut(s) 421, 444
AcoI YGGCCR 1 cut(s) 43
AfaI GTAC 2 cut(s) 233, 398
AfiI CCNNNNNNNGG 1 cut(s) 451
AgeI ACCGGT 1 cut(s) 179
AluBI AGCT 2 cut(s) 17, 392
AluI AGCT 2 cut(s) 17, 392
Alw21I GWGCWC 1 cut(s) 259
AlwI GGATC 2 cut(s) 421, 444
AoxI GGCC 3 cut(s) 43, 109, 329
AsiGI ACCGGT 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 25
AsuHPI GGTGA 2 cut(s) 85, 169
AsuNHI GCTAGC 1 cut(s) 388
AvaII GGWCC 1 cut(s) 25
BalI TGGCCA 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 259
BccI CCATC 1 cut(s) 321
BfaI CTAG 1 cut(s) 389
BmcAI AGTACT 1 cut(s) 398
Bme18I GGWCC 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 3 cut(s) 26, 27, 364
BmsI GCATC 1 cut(s) 321
BmtI GCTAGC 1 cut(s) 392
BpuEI CTTGAG 1 cut(s) 433
BsaJI CCNNGG 2 cut(s) 29, 96
BsaWI WCCGGW 2 cut(s) 179, 365
Bsc4I CCNNNNNNNGG 1 cut(s) 451
Bse118I RCCGGY 1 cut(s) 179
BseDI CCNNGG 2 cut(s) 29, 96
BseGI GGATG 1 cut(s) 332
BseLI CCNNNNNNNGG 1 cut(s) 451
BseMII CTCAG 1 cut(s) 384
BseYI CCCAGC 1 cut(s) 221
Bsh1236I CGCG 1 cut(s) 414
BshFI GGCC 3 cut(s) 45, 111, 331
BshTI ACCGGT 1 cut(s) 179
BsiHKAI GWGCWC 1 cut(s) 259
BsiSI CCGG 2 cut(s) 180, 366
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 1 cut(s) 451
BsmFI GGGAC 1 cut(s) 38
BsnI GGCC 3 cut(s) 45, 111, 331
Bsp1286I GDGCHC 1 cut(s) 259
Bsp1407I TGTACA 1 cut(s) 231
Bsp143I GATC 2 cut(s) 426, 436
Bsp19I CCATGG 1 cut(s) 96
BspANI GGCC 3 cut(s) 45, 111, 331
BspCNI CTCAG 1 cut(s) 385
BspFNI CGCG 1 cut(s) 414
BspLI GGNNCC 3 cut(s) 26, 27, 364
BspOI GCTAGC 1 cut(s) 392
BspPI GGATC 2 cut(s) 421, 444
BsrFI RCCGGY 1 cut(s) 179
BsrGI TGTACA 1 cut(s) 231
BssAI RCCGGY 1 cut(s) 179
BssECI CCNNGG 2 cut(s) 29, 96
BssMI GATC 2 cut(s) 426, 436
BssT1I CCWWGG 2 cut(s) 29, 96
Bst6I CTCTTC 4 cut(s) 95, 212, 217, 308
BstAUI TGTACA 1 cut(s) 231
BstC8I GCNNGC 2 cut(s) 255, 390
BstDEI CTNAG 3 cut(s) 128, 268, 393
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 332
BstFNI CGCG 1 cut(s) 414
BstKTI GATC 2 cut(s) 429, 439
BstMBI GATC 2 cut(s) 426, 436
BstMWI GCNNNNNNNGC 4 cut(s) 63, 380, 389, 411
BstUI CGCG 1 cut(s) 414
BstX2I RGATCY 1 cut(s) 426
BstYI RGATCY 1 cut(s) 426
BsuRI GGCC 3 cut(s) 45, 111, 331
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 332
Cac8I GCNNGC 2 cut(s) 255, 390
Cfr10I RCCGGY 1 cut(s) 179
Cfr13I GGNCC 1 cut(s) 25
Csp6I GTAC 2 cut(s) 232, 397
CspAI ACCGGT 1 cut(s) 179
CviAII CATG 3 cut(s) 97, 235, 316
CviJI RGCY 8 cut(s) 17, 34, 45, 111, 227, 331, 383, 392
CviKI_1 RGCY 8 cut(s) 17, 34, 45, 111, 227, 331, 383, 392
CviQI GTAC 2 cut(s) 232, 397
DdeI CTNAG 3 cut(s) 128, 268, 393
DpnI GATC 2 cut(s) 428, 438
DpnII GATC 2 cut(s) 426, 436
EaeI YGGCCR 1 cut(s) 43
Eam1104I CTCTTC 4 cut(s) 95, 212, 217, 308
EarI CTCTTC 4 cut(s) 95, 212, 217, 308
Eco130I CCWWGG 2 cut(s) 29, 96
Eco47I GGWCC 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 25
EcoT14I CCWWGG 2 cut(s) 29, 96
ErhI CCWWGG 2 cut(s) 29, 96
FaeI CATG 3 cut(s) 100, 238, 319
FaqI GGGAC 1 cut(s) 38
FatI CATG 3 cut(s) 96, 234, 315
FblI GTMKAC 1 cut(s) 138
FokI GGATG 1 cut(s) 339
FspBI CTAG 1 cut(s) 389
GsaI CCCAGC 1 cut(s) 225
HaeIII GGCC 3 cut(s) 45, 111, 331
HapII CCGG 2 cut(s) 180, 366
Hin1II CATG 3 cut(s) 100, 238, 319
HinfI GANTC 2 cut(s) 87, 369
HpaII CCGG 2 cut(s) 180, 366
HphI GGTGA 2 cut(s) 85, 169
Hpy166II GTNNAC 2 cut(s) 139, 232
Hpy8I GTNNAC 2 cut(s) 139, 232
HpyAV CCTTC 3 cut(s) 122, 184, 342
HpyCH4V TGCA 3 cut(s) 281, 312, 405
HpyF10VI GCNNNNNNNGC 4 cut(s) 63, 380, 389, 411
HpyF3I CTNAG 3 cut(s) 128, 268, 393
Hsp92II CATG 3 cut(s) 100, 238, 319
KflI GGGWCCC 1 cut(s) 25
Kzo9I GATC 2 cut(s) 426, 436
LmnI GCTCC 1 cut(s) 250
LpnPI CCDG 4 cut(s) 59, 193, 207, 379
LweI GCATC 1 cut(s) 321
MaeI CTAG 1 cut(s) 389
MalI GATC 2 cut(s) 428, 438
MboI GATC 2 cut(s) 426, 436
MboII GAAGA 5 cut(s) 82, 136, 199, 204, 295
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 1 cut(s) 259
MlsI TGGCCA 1 cut(s) 45
MluNI TGGCCA 1 cut(s) 45
MlyI GAGTC 2 cut(s) 81, 363
MnlI CCTC 6 cut(s) 68, 132, 208, 215, 317, 346
Mox20I TGGCCA 1 cut(s) 45
MscI TGGCCA 1 cut(s) 45
MseI TTAA 2 cut(s) 357, 408
MslI CAYNNNNRTG 1 cut(s) 262
Msp20I TGGCCA 1 cut(s) 45
MspA1I CMGCKG 1 cut(s) 17
MspI CCGG 2 cut(s) 180, 366
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 4 cut(s) 63, 380, 389, 411
NcoI CCATGG 1 cut(s) 96
NdeII GATC 2 cut(s) 426, 436
NheI GCTAGC 1 cut(s) 388
NlaIII CATG 3 cut(s) 100, 238, 319
NlaIV GGNNCC 3 cut(s) 26, 27, 364
PcsI WCGNNNNNNNCGW 1 cut(s) 418
PinAI ACCGGT 1 cut(s) 179
PleI GAGTC 2 cut(s) 81, 363
PpsI GAGTC 2 cut(s) 81, 363
PpuMI RGGWCCY 1 cut(s) 25
Psp5II RGGWCCY 1 cut(s) 25
PspFI CCCAGC 1 cut(s) 221
PspN4I GGNNCC 3 cut(s) 26, 27, 364
PspPI GGNCC 1 cut(s) 25
PspPPI RGGWCCY 1 cut(s) 25
PsuI RGATCY 1 cut(s) 426
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 2 cut(s) 233, 398
RsaNI GTAC 2 cut(s) 232, 397
RseI CAYNNNNRTG 1 cut(s) 262
SaqAI TTAA 2 cut(s) 357, 408
Sau3AI GATC 2 cut(s) 426, 436
Sau96I GGNCC 1 cut(s) 25
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 2 cut(s) 81, 363
SduI GDGCHC 1 cut(s) 259
SetI ASST 3 cut(s) 19, 176, 394
SfaNI GCATC 1 cut(s) 321
SinI GGWCC 1 cut(s) 25
SmiMI CAYNNNNRTG 1 cut(s) 262
SmlI CTYRAG 1 cut(s) 448
SmoI CTYRAG 1 cut(s) 448
SspMI CTAG 1 cut(s) 389
StyI CCWWGG 2 cut(s) 29, 96
TaqI TCGA 1 cut(s) 147
TatI WGTACW 2 cut(s) 231, 396
Tru1I TTAA 2 cut(s) 357, 408
Tru9I TTAA 2 cut(s) 357, 408
TspGWI ACGGA 1 cut(s) 274
VpaK11BI GGWCC 1 cut(s) 25
XmiI GTMKAC 1 cut(s) 138
XspI CTAG 1 cut(s) 389
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.