AT2G04540

3-oxoacyl- acyl-carrier-protein synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015277
Physical Location & Seq
Reverse (-)
1581376 .. 1584702
3327 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G04540.1

Sequence Viewer

Length: 1386 bp
ATGGCGACATCTAATCTCCGTAGACACTTGAGCGCAAGTCGCCTCCGCTTAAACCGCTTCATCTCTACTTCTTCTTCTTATCATTCACATCGCCGTGTTGTTGTCACTGGTCTAGGCATGGTGACTCCACTTGGTAGAGGCGTTGAAACAACGTGGAGGCGTTTAATTGATGGAGAATGTGGGATTAGAGGATTGACTCTTGATGATCTCAAGATGAAGTCTTTTGATGAAGAGACAAAGTTATATACTTTTGATCAGCTTTCTTCTAAAGTTGCTGCTTTTGTGCCTTATGGATCAAACCCTGGTGAATTTGATGAAGCCCTTTGGCTAAACTCTAAGGCAGTTGCGAATTTTATCGGATATGCTGTATGTGCTGCCGATGAAGCTTTGAGAGATGCAGAGTGGTTACCAACTGAAGAAGAAGAAAAAGAGAGAACAGGAGTCTCTATTGGTGGTGGAATTGGAAGTATATGTGATATTGTGGAGGCAGCGCAGCTGATTTGTGAAAAGAGGCTGCGGCGGCTTAGTCCGTTTTTCATTCCAAAAATATTGGTAAACATGGCATCTGGTCATGTGAGCATGAAGTATGGATTTCAGGGGCCAAATCATGCTGCTGTGACAGCTTGCGCAACTGGTGCACATTCTATAGGCGATGCCACTAGGATGATTCAATTTGGAGATGCAGATGTTATGGTGGCTGGTGGAACTGAGTCTAGCATTGATGCTCTGTCCGTAGCTGGATTCTCTAGATCAAGGGCTTTGTCAACTAAATTCAATTCTTCTCCACAAGAAGCTTCACGGCCTTTTGATTGTGACCGGGATGGTTTTGTGATAGGGGAAGGTTCAGGGGTTATAGTATTAGAGGAATATGAGCATGCAAAAAGACGAGGAGCAAAAATTTATGCTGAGCTTTGTGGATATGGGATGTCAGGCGATGCACACCATATTACTCAACCTCCTGAAGATGGAAAAGGAGCTGTTTTGGCCATGACGCGTGCCTTAAGACAGTCTGGTCTGTGTCCAAACCAAATTGATTATGTAAACGCACATGCAACATCAACCCCAATAGGCGATGCCGTGGAAGCGAGAGCTATCAAGACGGTATTCTCTGAACATGCTACTTCAGGCACCTTGGCATTCTCCTCCACCAAGGGGGCTACTGGTCATCTTCTTGGAGCAGCTGGAGCAGTGGAAGCTATTTTCAGTATCCTTGCTATACATCACGGGGTTGCTCCTATGACGCTGAATGTCAAGAATCCAGATCCCATCTTCGACAAGAGGTTCATGCCTTTAACCACTTCGAAAAAGATGTTAGTTAGGACAGCAATGTCGAATTCGTTTGGCTTTGGAGGAACAAATGCATCTTTGCTCTTTGCCTCTATCTAA

Protein Analysis

461

Amino Acids

49.38

Weight (kDa)

6.92

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ketoacyl-synt PF00109 31 - 293 2.8e-53 Beta-ketoacyl synthase, N-terminal domain
Ketoacyl-synt_C PF02801 301 - 417 2.5e-37 Beta-ketoacyl synthase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04540
fragaria_vesca FvH4_6g41780
malus_domestica MD09G1117200.v1.1
prunus_persica Prupe.3G206600_v2.0.a1 Prupe.3G206600_v2.0.a1
pyrus_communis pycom09g04220
rosa_chinensis RchiOBHm_Chr2g0157571
rosa_laevigata RLG00000020999
rosa_multiflora Rmu_sc0000195.1_g000042
rosa_roxburghii Rroxscaffold_2G00092110
rosa_rugosa Rorug02G0462200
rosa_samantha Rh2AG526300 Rh2BG541000 Rh2CG511000 Rh2DG549500
rosa_wichuraiana Rw2G043590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1011, 1327
Acc16I TGCGCA 1 cut(s) 628
AccB1I GGYRCC 1 cut(s) 1127
AccI GTMKAC 1 cut(s) 22
AccII CGCG 1 cut(s) 994
AciI CCGC 4 cut(s) 46, 55, 517, 520
AclWI GGATC 2 cut(s) 301, 1256
AcoI YGGCCR 1 cut(s) 984
AcsI RAATTY 5 cut(s) 308, 349, 770, 897, 1333
AcuI CTGAAG 3 cut(s) 435, 981, 1107
AfiI CCNNNNNNNGG 2 cut(s) 965, 1152
AflII CTTAAG 1 cut(s) 1000
AflIII ACRYGT 1 cut(s) 992
AgsI TTSAA 3 cut(s) 146, 671, 775
AjnI CCWGG 1 cut(s) 301
Alw21I GWGCWC 1 cut(s) 640
Alw26I GTCTC 2 cut(s) 227, 448
Alw44I GTGCAC 1 cut(s) 636
AlwI GGATC 2 cut(s) 301, 1256
AoxI GGCC 3 cut(s) 599, 800, 984
ApaLI GTGCAC 1 cut(s) 636
ApeKI GCWGC 7 cut(s) 275, 374, 488, 493, 514, 611, 1178
ApoI RAATTY 5 cut(s) 308, 349, 770, 897, 1333
AspLEI GCGC 3 cut(s) 35, 493, 629
AspS9I GGNCC 1 cut(s) 599
AsuC2I CCSGG 1 cut(s) 818
AsuHPI GGTGA 2 cut(s) 133, 317
AsuII TTCGAA 1 cut(s) 1301
BaeGI GKGCMC 1 cut(s) 640
BalI TGGCCA 1 cut(s) 986
BanI GGYRCC 1 cut(s) 1127
Bbv12I GWGCWC 1 cut(s) 640
BbvI GCAGC 7 cut(s) 262, 361, 500, 501, 505, 598, 1190
BccI CCATC 4 cut(s) 164, 815, 959, 1274
BceAI ACGGC 3 cut(s) 78, 815, 1061
BciT130I CCWGG 1 cut(s) 303
BciVI GTATCC 1 cut(s) 1217
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 1 cut(s) 818
BcoDI GTCTC 2 cut(s) 227, 448
BfaI CTAG 4 cut(s) 113, 660, 714, 747
BfmI CTRYAG 1 cut(s) 645
BfrI CTTAAG 1 cut(s) 1000
BfuI GTATCC 1 cut(s) 1217
BisI GCNGC 9 cut(s) 276, 375, 489, 494, 515, 518, 521, 612, 1179
BlpI GCTNAGC 1 cut(s) 906
BlsI GCNGC 9 cut(s) 277, 376, 490, 495, 516, 519, 522, 613, 1180
Bme1390I CCNGG 2 cut(s) 303, 818
BmgT120I GGNCC 1 cut(s) 599
BmiI GGNNCC 2 cut(s) 600, 1129
BmrFI CCNGG 2 cut(s) 303, 818
BmsI GCATC 8 cut(s) 385, 572, 643, 670, 712, 925, 1063, 1370
BpmI CTGGAG 1 cut(s) 1203
Bpu1102I GCTNAGC 1 cut(s) 906
Bpu14I TTCGAA 1 cut(s) 1301
BpuEI CTTGAG 2 cut(s) 49, 194
BpuMI CCSGG 1 cut(s) 818
BsaJI CCNNGG 4 cut(s) 301, 1077, 1131, 1149
BsaXI ACNNNNNCTCC 2 cut(s) 940, 970
Bsc4I CCNNNNNNNGG 2 cut(s) 965, 1152
Bse1I ACTGG 3 cut(s) 112, 637, 1165
Bse3DI GCAATG 1 cut(s) 1332
BseBI CCWGG 1 cut(s) 303
BseDI CCNNGG 4 cut(s) 301, 1077, 1131, 1149
BseGI GGATG 3 cut(s) 669, 826, 930
BseLI CCNNNNNNNGG 2 cut(s) 965, 1152
BseMI GCAATG 1 cut(s) 1332
BseMII CTCAG 2 cut(s) 699, 897
BseNI ACTGG 3 cut(s) 112, 637, 1165
BseRI GAGGAG 2 cut(s) 903, 1132
BseSI GKGCMC 1 cut(s) 640
BseXI GCAGC 7 cut(s) 262, 361, 500, 501, 505, 598, 1190
Bsh1236I CGCG 1 cut(s) 994
BshFI GGCC 3 cut(s) 601, 802, 986
BshNI GGYRCC 1 cut(s) 1127
BsiHKAI GWGCWC 1 cut(s) 640
BsiSI CCGG 1 cut(s) 817
BslI CCNNNNNNNGG 2 cut(s) 965, 1152
BsmAI GTCTC 2 cut(s) 227, 448
BsmI GAATGC 1 cut(s) 1136
BsnI GGCC 3 cut(s) 601, 802, 986
Bsp119I TTCGAA 1 cut(s) 1301
Bsp1286I GDGCHC 1 cut(s) 640
Bsp143I GATC 5 cut(s) 205, 253, 293, 749, 1261
Bsp1720I GCTNAGC 1 cut(s) 906
BspACI CCGC 4 cut(s) 46, 55, 517, 520
BspANI GGCC 3 cut(s) 601, 802, 986
BspCNI CTCAG 2 cut(s) 700, 898
BspFNI CGCG 1 cut(s) 994
BspLI GGNNCC 2 cut(s) 600, 1129
BspPI GGATC 2 cut(s) 301, 1256
BspT104I TTCGAA 1 cut(s) 1301
BspT107I GGYRCC 1 cut(s) 1127
BspTI CTTAAG 1 cut(s) 1000
BsrDI GCAATG 1 cut(s) 1332
BsrI ACTGG 3 cut(s) 112, 637, 1165
BssECI CCNNGG 4 cut(s) 301, 1077, 1131, 1149
BssMI GATC 5 cut(s) 205, 253, 293, 749, 1261
BssT1I CCWWGG 2 cut(s) 1131, 1149
Bst2UI CCWGG 1 cut(s) 303
Bst4CI ACNGT 2 cut(s) 1008, 1102
Bst6I CTCTTC 1 cut(s) 225
BstAFI CTTAAG 1 cut(s) 1000
BstAPI GCANNNNNTGC 1 cut(s) 635
BstBI TTCGAA 1 cut(s) 1301
BstC8I GCNNGC 3 cut(s) 625, 876, 996
BstDEI CTNAG 4 cut(s) 336, 524, 708, 906
BstDSI CCRYGG 1 cut(s) 1077
BstEII GGTNACC 1 cut(s) 405
BstF5I GGATG 3 cut(s) 669, 826, 930
BstFNI CGCG 1 cut(s) 994
BstHHI GCGC 3 cut(s) 35, 493, 629
BstKTI GATC 5 cut(s) 208, 256, 296, 752, 1264
BstMAI GTCTC 2 cut(s) 227, 448
BstMBI GATC 5 cut(s) 205, 253, 293, 749, 1261
BstNI CCWGG 1 cut(s) 303
BstNSI RCATGY 3 cut(s) 878, 1052, 1118
BstPI GGTNACC 1 cut(s) 405
BstSCI CCNGG 2 cut(s) 301, 816
BstSFI CTRYAG 1 cut(s) 645
BstSLI GKGCMC 1 cut(s) 640
BstUI CGCG 1 cut(s) 994
BstV1I GCAGC 7 cut(s) 262, 361, 500, 501, 505, 598, 1190
BstX2I RGATCY 1 cut(s) 1261
BstYI RGATCY 1 cut(s) 1261
BsuI GTATCC 1 cut(s) 1217
BsuRI GGCC 3 cut(s) 601, 802, 986
BtgI CCRYGG 1 cut(s) 1077
BtgZI GCGATG 4 cut(s) 74, 666, 948, 1086
BtsCI GGATG 3 cut(s) 669, 826, 930
BtsI GCAGTG 1 cut(s) 1194
BtsIMutI CAGTG 2 cut(s) 105, 1194
Cac8I GCNNGC 3 cut(s) 625, 876, 996
CfoI GCGC 3 cut(s) 35, 493, 629
Cfr13I GGNCC 1 cut(s) 599
CseI GACGC 2 cut(s) 1000, 1249
DdeI CTNAG 4 cut(s) 336, 524, 708, 906
DpnI GATC 5 cut(s) 207, 255, 295, 751, 1263
DpnII GATC 5 cut(s) 205, 253, 293, 749, 1261
DrdI GACNNNNNNGTC 2 cut(s) 1011, 1327
DseDI GACNNNNNNGTC 2 cut(s) 1011, 1327
EaeI YGGCCR 1 cut(s) 984
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Eco130I CCWWGG 2 cut(s) 1131, 1149
Eco57I CTGAAG 3 cut(s) 435, 981, 1107
Eco91I GGTNACC 1 cut(s) 405
EcoO65I GGTNACC 1 cut(s) 405
EcoRI GAATTC 1 cut(s) 1333
EcoRII CCWGG 1 cut(s) 301
EcoT14I CCWWGG 2 cut(s) 1131, 1149
EcoT22I ATGCAT 1 cut(s) 1363
ErhI CCWWGG 2 cut(s) 1131, 1149
FbaI TGATCA 1 cut(s) 253
FblI GTMKAC 1 cut(s) 22
Fnu4HI GCNGC 9 cut(s) 276, 375, 489, 494, 515, 518, 521, 612, 1179
FokI GGATG 3 cut(s) 676, 833, 937
Fsp4HI GCNGC 9 cut(s) 276, 375, 489, 494, 515, 518, 521, 612, 1179
FspBI CTAG 4 cut(s) 113, 660, 714, 747
FspI TGCGCA 1 cut(s) 628
GlaI GCGC 3 cut(s) 34, 492, 628
GluI GCNGC 9 cut(s) 276, 375, 489, 494, 515, 518, 521, 612, 1179
GsuI CTGGAG 1 cut(s) 1203
HaeIII GGCC 3 cut(s) 601, 802, 986
HapII CCGG 1 cut(s) 817
HgaI GACGC 2 cut(s) 1000, 1249
HhaI GCGC 3 cut(s) 35, 493, 629
Hin6I GCGC 3 cut(s) 33, 491, 627
HinP1I GCGC 3 cut(s) 33, 491, 627
HincII GTYRAC 1 cut(s) 765
HindII GTYRAC 1 cut(s) 765
HindIII AAGCTT 2 cut(s) 384, 792
HinfI GANTC 7 cut(s) 124, 196, 441, 667, 710, 741, 1255
HpaII CCGG 1 cut(s) 817
HphI GGTGA 2 cut(s) 133, 317
Hpy166II GTNNAC 5 cut(s) 23, 556, 638, 765, 1042
Hpy188I TCNGA 2 cut(s) 359, 1111
Hpy188III TCNNGA 7 cut(s) 200, 211, 747, 959, 1096, 1252, 1259
Hpy8I GTNNAC 5 cut(s) 23, 556, 638, 765, 1042
HpyAV CCTTC 1 cut(s) 833
HpyCH4III ACNGT 2 cut(s) 1008, 1102
HpyCH4IV ACGT 1 cut(s) 152
HpyCH4V TGCA 7 cut(s) 398, 638, 683, 878, 938, 1052, 1361
HpyF3I CTNAG 4 cut(s) 336, 524, 708, 906
HpySE526I ACGT 1 cut(s) 152
HspAI GCGC 3 cut(s) 33, 491, 627
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 5 cut(s) 205, 253, 293, 749, 1261
LmnI GCTCC 5 cut(s) 890, 974, 1175, 1184, 1237
Lsp1109I GCAGC 7 cut(s) 262, 361, 500, 501, 505, 598, 1190
LweI GCATC 8 cut(s) 385, 572, 643, 670, 712, 925, 1063, 1370
MaeI CTAG 4 cut(s) 113, 660, 714, 747
MaeII ACGT 1 cut(s) 152
MaeIII GTNAC 5 cut(s) 103, 121, 405, 616, 812
MalI GATC 5 cut(s) 207, 255, 295, 751, 1263
MboI GATC 5 cut(s) 205, 253, 293, 749, 1261
MflI RGATCY 1 cut(s) 1261
MhlI GDGCHC 1 cut(s) 640
MlsI TGGCCA 1 cut(s) 986
MluI ACGCGT 1 cut(s) 992
MluNI TGGCCA 1 cut(s) 986
MlyI GAGTC 4 cut(s) 118, 190, 450, 719
Mox20I TGGCCA 1 cut(s) 986
Mph1103I ATGCAT 1 cut(s) 1363
MscI TGGCCA 1 cut(s) 986
MseI TTAA 4 cut(s) 50, 164, 1001, 1292
MslI CAYNNNNRTG 2 cut(s) 93, 662
Msp20I TGGCCA 1 cut(s) 986
MspA1I CMGCKG 2 cut(s) 496, 1181
MspCI CTTAAG 1 cut(s) 1000
MspI CCGG 1 cut(s) 817
MspR9I CCNGG 2 cut(s) 303, 818
Mva1269I GAATGC 1 cut(s) 1136
MvaI CCWGG 1 cut(s) 303
MvnI CGCG 1 cut(s) 994
NciI CCSGG 1 cut(s) 818
NdeII GATC 5 cut(s) 205, 253, 293, 749, 1261
NlaIV GGNNCC 2 cut(s) 600, 1129
NmuCI GTSAC 4 cut(s) 103, 121, 616, 812
NsbI TGCGCA 1 cut(s) 628
NsiI ATGCAT 1 cut(s) 1363
NspI RCATGY 3 cut(s) 878, 1052, 1118
NspV TTCGAA 1 cut(s) 1301
PaeI GCATGC 1 cut(s) 878
PctI GAATGC 1 cut(s) 1136
PfeI GAWTC 3 cut(s) 667, 741, 1255
PkrI GCNGC 9 cut(s) 277, 376, 490, 495, 516, 519, 522, 613, 1180
PleI GAGTC 4 cut(s) 118, 190, 449, 718
PpsI GAGTC 4 cut(s) 118, 190, 449, 718
Psp6I CCWGG 1 cut(s) 301
PspEI GGTNACC 1 cut(s) 405
PspGI CCWGG 1 cut(s) 301
PspN4I GGNNCC 2 cut(s) 600, 1129
PspPI GGNCC 1 cut(s) 599
PsuI RGATCY 1 cut(s) 1261
PvuII CAGCTG 2 cut(s) 496, 1181
RseI CAYNNNNRTG 2 cut(s) 93, 662
SaqAI TTAA 4 cut(s) 50, 164, 1001, 1292
SatI GCNGC 9 cut(s) 276, 375, 489, 494, 515, 518, 521, 612, 1179
Sau3AI GATC 5 cut(s) 205, 253, 293, 749, 1261
Sau96I GGNCC 1 cut(s) 599
SchI GAGTC 4 cut(s) 118, 190, 450, 719
ScrFI CCNGG 2 cut(s) 303, 818
SduI GDGCHC 1 cut(s) 640
SfaNI GCATC 8 cut(s) 385, 572, 643, 670, 712, 925, 1063, 1370
SfcI CTRYAG 1 cut(s) 645
SfuI TTCGAA 1 cut(s) 1301
SmiMI CAYNNNNRTG 2 cut(s) 93, 662
SmlI CTYRAG 3 cut(s) 28, 209, 1000
SmoI CTYRAG 3 cut(s) 28, 209, 1000
SphI GCATGC 1 cut(s) 878
SsiI CCGC 4 cut(s) 46, 55, 517, 520
SspI AATATT 1 cut(s) 549
SspMI CTAG 4 cut(s) 113, 660, 714, 747
StyD4I CCNGG 2 cut(s) 301, 816
StyI CCWWGG 2 cut(s) 1131, 1149
TaaI ACNGT 2 cut(s) 1008, 1102
TaiI ACGT 1 cut(s) 155
TaqI TCGA 3 cut(s) 1272, 1301, 1331
TauI GCSGC 2 cut(s) 520, 523
TfiI GAWTC 3 cut(s) 667, 741, 1255
Tru1I TTAA 4 cut(s) 50, 164, 1001, 1292
Tru9I TTAA 4 cut(s) 50, 164, 1001, 1292
TscAI CASTG 2 cut(s) 112, 1194
TseFI GTSAC 4 cut(s) 103, 121, 616, 812
TseI GCWGC 7 cut(s) 275, 374, 488, 493, 514, 611, 1178
Tsp45I GTSAC 4 cut(s) 103, 121, 616, 812
TspDTI ATGAA 8 cut(s) 49, 230, 243, 330, 396, 526, 596, 1273
TspGWI ACGGA 3 cut(s) 8, 519, 721
TspRI CASTG 2 cut(s) 112, 1194
Vha464I CTTAAG 1 cut(s) 1000
VneI GTGCAC 1 cut(s) 636
XapI RAATTY 5 cut(s) 308, 349, 770, 897, 1333
XbaI TCTAGA 1 cut(s) 746
XceI RCATGY 3 cut(s) 878, 1052, 1118
XmiI GTMKAC 1 cut(s) 22
XspI CTAG 4 cut(s) 113, 660, 714, 747
Zsp2I ATGCAT 1 cut(s) 1363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.