FvH4_6g41780

Belongs to the beta-ketoacyl-ACP synthases family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015277
Physical Location & Seq
Reverse (-)
32748213 .. 32753585
5373 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41780.t1

Sequence Viewer

Length: 1410 bp
ATGAGAGCTCTGAAAGCTATGGCTGCTTCTTCTGGTTGGCGCAAGCTCTTCACTCGTCGCATTTCATCTTCTTCTTTCGACCCACCTCCACTTGTTCACTCTCGCAGAGTCGTCGTTACTGGAATGGGCCTGGTGACGCCATTGGGGTGTGGGGTGGAGACTACATGGAAGAGACTCGTAGACGGTGCCTGTGGCATTAGGGGATTGACACTTGAAGATCTTAAGATGAATGGGTTTGATGCAGAGACTCAAGCTCACTCCTTTGACCAGCTCACTTCCAAAGTCGCCGCTATTGTGCCCACCGGAACCAATTTAGGTGAATTTAATGAAGACTTGTGGCTCAATTCTAAGGAGCATCGTTCGATTGCAAGGTTTATAGGGTATGCATTGTGTGCTGCTGATGAGGCGCTTAGAGATGCTAATTGGATGCCTACTGAGGCTGAACACAAAGAAAGAACGGGTGTCTCAGTTGGTGGGGGAATTGGAAGCATCAGTGACATATTGGATGCTGCACAGTTGATCTGTGAGAAGCGTCTACGTCGGCTTAGTCCTTTTTTCATCCCGCGGATATTGATCAACATGGCAGCTGGTCATGTCAGCATGAAATATGGATTCCAGGGACCAAACCATGCTGCAGTAACAGCATGTGCCACTGGTGCACATTCTCTGGGTGATGCCACAAGGATGATTCAGTTTGGAGATTCAGATGTTATGGTGGCTGGAGGCACAGAGTCTAGTATTGATGCTTTATCAATTGCAGGATTTTGCAGGTCAAGGGCTTTGTCTACAAAGTACAATTCCTCCCCAGAAGAAGCATCACGACCTTTTGACTGTGGTCGTGATGGGTTTGTGATAGGTGAAGGTTCTGGTATCTTGGTGTTGGAGGAACTTGAGCATGCAAAGAATCGAGGTGCAAAAATCTATGCAGAAGTTCGTGGTTATGGGATGTCAGGTGATGCATATCACATTACTCAACCACATACTGACGGAAGAGGTGCCATTCTAGCCATGACTAGCGCATTGAGACAGTCTGGGCTTCATCCTCACCAAGTGGATTATGTAAATGCCCATGCTACCTCGACACCTTTGGGTGATTCAATAGAAGCCAATGCTATCAGGAATGTTTTCTCTGAACATGCAACATCAGGTTCTCTAGCTCTGTCCTCCACAAAGGGCGCTATTGGTCATCTCCTTGGTGCAGCTGGGGCTGTTGAAGCAATATTTTCTATATTGGCCATACACCATGGAGTTGCACCATTAACACTTAATCTGTCAAAACCAGACCCACTATTTATAGATGCTTTCATGCCTTTGACAGCTTCAAAGGAAATGAATATAAGAGCAGCTTTGTCCAACTCTTTTGGCTTTGGAGGAACAAATGCATCCCTGCTGTTTACGTCTCCTCCCTGA

Protein Analysis

470

Amino Acids

49.74

Weight (kDa)

6.63

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ketoacyl-synt PF00109 35 - 300 6.2e-53 Beta-ketoacyl synthase, N-terminal domain
Ketoacyl-synt_C PF02801 308 - 424 2e-39 Beta-ketoacyl synthase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015277)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G04540
fragaria_vesca FvH4_6g41780
malus_domestica MD09G1117200.v1.1
prunus_persica Prupe.3G206600_v2.0.a1 Prupe.3G206600_v2.0.a1
pyrus_communis pycom09g04220
rosa_chinensis RchiOBHm_Chr2g0157571
rosa_laevigata RLG00000020999
rosa_multiflora Rmu_sc0000195.1_g000042
rosa_roxburghii Rroxscaffold_2G00092110
rosa_rugosa Rorug02G0462200
rosa_samantha Rh2AG526300 Rh2BG541000 Rh2CG511000 Rh2DG549500
rosa_wichuraiana Rw2G043590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 759
AccB1I GGYRCC 2 cut(s) 185, 995
AccI GTMKAC 3 cut(s) 180, 535, 785
AccII CGCG 1 cut(s) 565
AciI CCGC 3 cut(s) 288, 563, 565
AcoI YGGCCR 1 cut(s) 1233
AcsI RAATTY 1 cut(s) 320
AcyI GRCGYC 1 cut(s) 137
AdeI CACNNNGTG 1 cut(s) 1051
AfaI GTAC 1 cut(s) 794
AflII CTTAAG 1 cut(s) 221
AgsI TTSAA 4 cut(s) 215, 1098, 1214, 1323
AjnI CCWGG 2 cut(s) 129, 615
Alw21I GWGCWC 2 cut(s) 10, 661
Alw26I GTCTC 6 cut(s) 152, 166, 239, 469, 1018, 1404
Alw44I GTGCAC 1 cut(s) 657
AoxI GGCC 2 cut(s) 127, 1233
ApaLI GTGCAC 1 cut(s) 657
ApeKI GCWGC 7 cut(s) 23, 395, 509, 584, 632, 1199, 1343
ApoI RAATTY 1 cut(s) 320
Asp700I GAANNNNTTC 1 cut(s) 1124
AspLEI GCGC 4 cut(s) 42, 409, 1019, 1178
AspS9I GGNCC 2 cut(s) 127, 620
AsuHPI GGTGA 7 cut(s) 145, 329, 683, 869, 965, 1037, 1103
AvaII GGWCC 1 cut(s) 620
BaeGI GKGCMC 2 cut(s) 300, 661
BalI TGGCCA 1 cut(s) 1235
BanI GGYRCC 2 cut(s) 185, 995
BanII GRGCYC 1 cut(s) 10
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 2 cut(s) 10, 661
BbvI GCAGC 7 cut(s) 10, 382, 496, 596, 619, 1211, 1355
BccI CCATC 1 cut(s) 836
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BciT130I CCWGG 2 cut(s) 131, 617
BclI TGATCA 1 cut(s) 573
BcoDI GTCTC 6 cut(s) 152, 166, 239, 469, 1018, 1404
BfaI CTAG 4 cut(s) 735, 1004, 1014, 1154
BfmI CTRYAG 1 cut(s) 633
BfoI RGCGCY 2 cut(s) 410, 1179
BfrI CTTAAG 1 cut(s) 221
BfuAI ACCTGC 1 cut(s) 759
BglII AGATCT 1 cut(s) 217
BisI GCNGC 8 cut(s) 24, 288, 396, 510, 585, 633, 1200, 1344
BlsI GCNGC 8 cut(s) 25, 289, 397, 511, 586, 634, 1201, 1345
Bme1390I CCNGG 2 cut(s) 131, 617
Bme18I GGWCC 1 cut(s) 620
BmgT120I GGNCC 2 cut(s) 127, 620
BmiI GGNNCC 4 cut(s) 187, 307, 621, 997
BmrFI CCNGG 2 cut(s) 131, 617
BoxI GACNNNNGTC 1 cut(s) 834
BpiI GAAGAC 1 cut(s) 336
BpmI CTGGAG 1 cut(s) 741
BpuEI CTTGAG 2 cut(s) 234, 911
BsaBI GATNNNNATC 2 cut(s) 572, 960
BsaHI GRCGYC 1 cut(s) 137
BsaJI CCNNGG 4 cut(s) 563, 616, 1192, 1243
BsaWI WCCGGW 1 cut(s) 302
BsaXI ACNNNNNCTCC 3 cut(s) 785, 815, 1387
Bse1I ACTGG 2 cut(s) 124, 658
Bse8I GATNNNNATC 2 cut(s) 572, 960
BseBI CCWGG 2 cut(s) 131, 617
BseDI CCNNGG 4 cut(s) 563, 616, 1192, 1243
BseGI GGATG 7 cut(s) 432, 511, 558, 690, 951, 1039, 1382
BseJI GATNNNNATC 2 cut(s) 572, 960
BseMII CTCAG 2 cut(s) 426, 480
BseNI ACTGG 2 cut(s) 124, 658
BseRI GAGGAG 1 cut(s) 1392
BseSI GKGCMC 2 cut(s) 300, 661
BseXI GCAGC 7 cut(s) 10, 382, 496, 596, 619, 1211, 1355
BseYI CCCAGC 1 cut(s) 1202
BsgI GTGCAG 2 cut(s) 495, 1218
Bsh1236I CGCG 1 cut(s) 565
BshFI GGCC 2 cut(s) 129, 1235
BshNI GGYRCC 2 cut(s) 185, 995
BsiHKAI GWGCWC 2 cut(s) 10, 661
BsiSI CCGG 1 cut(s) 303
BslFI GGGAC 1 cut(s) 633
BsmAI GTCTC 6 cut(s) 152, 166, 239, 469, 1018, 1404
BsmBI CGTCTC 1 cut(s) 1404
BsmFI GGGAC 1 cut(s) 633
BsnI GGCC 2 cut(s) 129, 1235
Bsp1286I GDGCHC 3 cut(s) 10, 300, 661
Bsp143I GATC 3 cut(s) 217, 519, 573
Bsp19I CCATGG 1 cut(s) 1243
BspACI CCGC 3 cut(s) 288, 563, 565
BspANI GGCC 2 cut(s) 129, 1235
BspCNI CTCAG 2 cut(s) 427, 479
BspFNI CGCG 1 cut(s) 565
BspLI GGNNCC 4 cut(s) 187, 307, 621, 997
BspMAI CTGCAG 1 cut(s) 637
BspMI ACCTGC 1 cut(s) 759
BspQI GCTCTTC 1 cut(s) 53
BspT107I GGYRCC 2 cut(s) 185, 995
BspTI CTTAAG 1 cut(s) 221
BsrI ACTGG 2 cut(s) 124, 658
BssECI CCNNGG 4 cut(s) 563, 616, 1192, 1243
BssMI GATC 3 cut(s) 217, 519, 573
BssNI GRCGYC 1 cut(s) 137
BssT1I CCWWGG 2 cut(s) 1192, 1243
Bst2UI CCWGG 2 cut(s) 131, 617
Bst4CI ACNGT 4 cut(s) 185, 516, 833, 1029
Bst6I CTCTTC 3 cut(s) 53, 164, 985
BstACI GRCGYC 1 cut(s) 137
BstAFI CTTAAG 1 cut(s) 221
BstAPI GCANNNNNTGC 1 cut(s) 392
BstC8I GCNNGC 2 cut(s) 44, 897
BstDEI CTNAG 5 cut(s) 348, 410, 435, 466, 545
BstDSI CCRYGG 2 cut(s) 563, 1243
BstF5I GGATG 7 cut(s) 432, 511, 558, 690, 951, 1039, 1382
BstFNI CGCG 1 cut(s) 565
BstH2I RGCGCY 2 cut(s) 410, 1179
BstHHI GCGC 4 cut(s) 42, 409, 1019, 1178
BstKTI GATC 3 cut(s) 220, 522, 576
BstMAI GTCTC 6 cut(s) 152, 166, 239, 469, 1018, 1404
BstMBI GATC 3 cut(s) 217, 519, 573
BstMWI GCNNNNNNNGC 9 cut(s) 14, 23, 392, 404, 641, 656, 1004, 1205, 1214
BstNI CCWGG 2 cut(s) 131, 617
BstNSI RCATGY 3 cut(s) 648, 899, 1139
BstPAI GACNNNNGTC 1 cut(s) 834
BstSCI CCNGG 2 cut(s) 129, 615
BstSFI CTRYAG 1 cut(s) 633
BstSLI GKGCMC 2 cut(s) 300, 661
BstUI CGCG 1 cut(s) 565
BstV1I GCAGC 7 cut(s) 10, 382, 496, 596, 619, 1211, 1355
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuRI GGCC 2 cut(s) 129, 1235
BtgI CCRYGG 2 cut(s) 563, 1243
BtsCI GGATG 7 cut(s) 432, 511, 558, 690, 951, 1039, 1382
BtsIMutI CAGTG 2 cut(s) 499, 651
BveI ACCTGC 1 cut(s) 759
Cac8I GCNNGC 2 cut(s) 44, 897
CfoI GCGC 4 cut(s) 42, 409, 1019, 1178
Cfr13I GGNCC 2 cut(s) 127, 620
Cfr42I CCGCGG 1 cut(s) 566
CseI GACGC 2 cut(s) 145, 521
Csp6I GTAC 1 cut(s) 793
CviQI GTAC 1 cut(s) 793
DdeI CTNAG 5 cut(s) 348, 410, 435, 466, 545
DpnI GATC 3 cut(s) 219, 521, 575
DpnII GATC 3 cut(s) 217, 519, 573
DraIII CACNNNGTG 1 cut(s) 1051
EaeI YGGCCR 1 cut(s) 1233
Eam1104I CTCTTC 3 cut(s) 53, 164, 985
EarI CTCTTC 3 cut(s) 53, 164, 985
Ecl136II GAGCTC 1 cut(s) 8
Eco130I CCWWGG 2 cut(s) 1192, 1243
Eco24I GRGCYC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 620
Eco53kI GAGCTC 1 cut(s) 8
EcoICRI GAGCTC 1 cut(s) 8
EcoRII CCWGG 2 cut(s) 129, 615
EcoT14I CCWWGG 2 cut(s) 1192, 1243
EcoT22I ATGCAT 3 cut(s) 388, 961, 1384
EcoT38I GRGCYC 1 cut(s) 10
ErhI CCWWGG 2 cut(s) 1192, 1243
Esp3I CGTCTC 1 cut(s) 1404
FalI AAGNNNNNCTT 2 cut(s) 1330, 1362
FaqI GGGAC 1 cut(s) 633
FauI CCCGC 1 cut(s) 570
FbaI TGATCA 1 cut(s) 573
FblI GTMKAC 3 cut(s) 180, 535, 785
Fnu4HI GCNGC 8 cut(s) 24, 288, 396, 510, 585, 633, 1200, 1344
FokI GGATG 7 cut(s) 439, 518, 545, 697, 958, 1026, 1369
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 8 cut(s) 24, 288, 396, 510, 585, 633, 1200, 1344
FspBI CTAG 4 cut(s) 735, 1004, 1014, 1154
GlaI GCGC 4 cut(s) 41, 408, 1018, 1177
GluI GCNGC 8 cut(s) 24, 288, 396, 510, 585, 633, 1200, 1344
GsaI CCCAGC 1 cut(s) 1206
GsuI CTGGAG 1 cut(s) 741
HaeII RGCGCY 2 cut(s) 410, 1179
HaeIII GGCC 2 cut(s) 129, 1235
HapII CCGG 1 cut(s) 303
HgaI GACGC 2 cut(s) 145, 521
HhaI GCGC 4 cut(s) 42, 409, 1019, 1178
Hin1I GRCGYC 1 cut(s) 137
Hin6I GCGC 4 cut(s) 40, 407, 1017, 1176
HinP1I GCGC 4 cut(s) 40, 407, 1017, 1176
HinfI GANTC 9 cut(s) 108, 174, 247, 612, 688, 701, 731, 904, 1094
HpaII CCGG 1 cut(s) 303
HphI GGTGA 7 cut(s) 145, 329, 683, 869, 965, 1037, 1103
Hpy166II GTNNAC 6 cut(s) 97, 181, 536, 659, 786, 1395
Hpy188I TCNGA 3 cut(s) 12, 706, 1132
Hpy188III TCNNGA 3 cut(s) 819, 839, 1117
Hpy8I GTNNAC 6 cut(s) 97, 181, 536, 659, 786, 1395
Hpy99I CGWCG 3 cut(s) 60, 116, 543
HpyAV CCTTC 1 cut(s) 854
HpyCH4III ACNGT 4 cut(s) 185, 516, 833, 1029
HpyCH4IV ACGT 2 cut(s) 538, 1397
HpyF10VI GCNNNNNNNGC 9 cut(s) 14, 23, 392, 404, 641, 656, 1004, 1205, 1214
HpyF3I CTNAG 5 cut(s) 348, 410, 435, 466, 545
HpySE526I ACGT 2 cut(s) 538, 1397
Hsp92I GRCGYC 1 cut(s) 137
HspAI GCGC 4 cut(s) 40, 407, 1017, 1176
Ksp22I TGATCA 1 cut(s) 573
KspI CCGCGG 1 cut(s) 566
Kzo9I GATC 3 cut(s) 217, 519, 573
LguI GCTCTTC 1 cut(s) 53
LmnI GCTCC 1 cut(s) 352
Lsp1109I GCAGC 7 cut(s) 10, 382, 496, 596, 619, 1211, 1355
MaeI CTAG 4 cut(s) 735, 1004, 1014, 1154
MaeII ACGT 2 cut(s) 538, 1397
MaeIII GTNAC 4 cut(s) 115, 133, 494, 637
MalI GATC 3 cut(s) 219, 521, 575
MboI GATC 3 cut(s) 217, 519, 573
MboII GAAGA 9 cut(s) 21, 40, 60, 63, 181, 227, 341, 821, 1002
MfeI CAATTG 1 cut(s) 753
MflI RGATCY 1 cut(s) 217
MhlI GDGCHC 3 cut(s) 10, 300, 661
MlsI TGGCCA 1 cut(s) 1235
MluCI AATT 7 cut(s) 310, 320, 343, 421, 480, 753, 796
MluNI TGGCCA 1 cut(s) 1235
MlyI GAGTC 4 cut(s) 117, 168, 241, 740
MmeI TCCRAC 2 cut(s) 861, 1377
Mox20I TGGCCA 1 cut(s) 1235
Mph1103I ATGCAT 3 cut(s) 388, 961, 1384
MroXI GAANNNNTTC 1 cut(s) 1124
MscI TGGCCA 1 cut(s) 1235
MseI TTAA 4 cut(s) 222, 324, 1259, 1266
MslI CAYNNNNRTG 2 cut(s) 145, 683
Msp20I TGGCCA 1 cut(s) 1235
MspA1I CMGCKG 3 cut(s) 565, 587, 1202
MspCI CTTAAG 1 cut(s) 221
MspI CCGG 1 cut(s) 303
MspR9I CCNGG 2 cut(s) 131, 617
MunI CAATTG 1 cut(s) 753
MvaI CCWGG 2 cut(s) 131, 617
MvnI CGCG 1 cut(s) 565
MwoI GCNNNNNNNGC 9 cut(s) 14, 23, 392, 404, 641, 656, 1004, 1205, 1214
NcoI CCATGG 1 cut(s) 1243
NdeII GATC 3 cut(s) 217, 519, 573
NlaIV GGNNCC 4 cut(s) 187, 307, 621, 997
NmuCI GTSAC 2 cut(s) 133, 494
NsiI ATGCAT 3 cut(s) 388, 961, 1384
NspI RCATGY 3 cut(s) 648, 899, 1139
PaeI GCATGC 1 cut(s) 899
PciSI GCTCTTC 1 cut(s) 53
PdmI GAANNNNTTC 1 cut(s) 1124
PfeI GAWTC 5 cut(s) 612, 688, 701, 904, 1094
PkrI GCNGC 8 cut(s) 25, 289, 397, 511, 586, 634, 1201, 1345
PleI GAGTC 4 cut(s) 116, 168, 241, 739
PpsI GAGTC 4 cut(s) 116, 168, 241, 739
PshAI GACNNNNGTC 1 cut(s) 834
Psp124BI GAGCTC 1 cut(s) 10
Psp6I CCWGG 2 cut(s) 129, 615
PspFI CCCAGC 1 cut(s) 1202
PspGI CCWGG 2 cut(s) 129, 615
PspN4I GGNNCC 4 cut(s) 187, 307, 621, 997
PspPI GGNCC 2 cut(s) 127, 620
PstI CTGCAG 1 cut(s) 637
PsuI RGATCY 1 cut(s) 217
PvuII CAGCTG 2 cut(s) 587, 1202
RsaI GTAC 1 cut(s) 794
RsaNI GTAC 1 cut(s) 793
RseI CAYNNNNRTG 2 cut(s) 145, 683
SacI GAGCTC 1 cut(s) 10
SacII CCGCGG 1 cut(s) 566
SapI GCTCTTC 1 cut(s) 53
SaqAI TTAA 4 cut(s) 222, 324, 1259, 1266
SatI GCNGC 8 cut(s) 24, 288, 396, 510, 585, 633, 1200, 1344
Sau3AI GATC 3 cut(s) 217, 519, 573
Sau96I GGNCC 2 cut(s) 127, 620
SchI GAGTC 4 cut(s) 117, 168, 241, 740
ScrFI CCNGG 2 cut(s) 131, 617
SduI GDGCHC 3 cut(s) 10, 300, 661
SfcI CTRYAG 1 cut(s) 633
Sfr303I CCGCGG 1 cut(s) 566
SgrBI CCGCGG 1 cut(s) 566
SinI GGWCC 1 cut(s) 620
SmiMI CAYNNNNRTG 2 cut(s) 145, 683
SmlI CTYRAG 3 cut(s) 221, 249, 890
SmoI CTYRAG 3 cut(s) 221, 249, 890
SphI GCATGC 1 cut(s) 899
Sse9I AATT 7 cut(s) 310, 320, 343, 421, 480, 753, 796
SsiI CCGC 3 cut(s) 288, 563, 565
SspI AATATT 1 cut(s) 1221
SspMI CTAG 4 cut(s) 735, 1004, 1014, 1154
SstI GAGCTC 1 cut(s) 10
StyD4I CCNGG 2 cut(s) 129, 615
StyI CCWWGG 2 cut(s) 1192, 1243
TaaI ACNGT 4 cut(s) 185, 516, 833, 1029
TaiI ACGT 2 cut(s) 541, 1400
TaqI TCGA 4 cut(s) 78, 362, 907, 1079
TasI AATT 7 cut(s) 310, 320, 343, 421, 480, 753, 796
TatI WGTACW 1 cut(s) 792
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 5 cut(s) 612, 688, 701, 904, 1094
Tru1I TTAA 4 cut(s) 222, 324, 1259, 1266
Tru9I TTAA 4 cut(s) 222, 324, 1259, 1266
TscAI CASTG 2 cut(s) 499, 658
TseFI GTSAC 2 cut(s) 133, 494
TseI GCWGC 7 cut(s) 23, 395, 509, 584, 632, 1199, 1343
Tsp45I GTSAC 2 cut(s) 133, 494
TspDTI ATGAA 8 cut(s) 54, 242, 342, 547, 617, 1028, 1294, 1346
TspGWI ACGGA 1 cut(s) 1002
TspRI CASTG 2 cut(s) 499, 658
Vha464I CTTAAG 1 cut(s) 221
VneI GTGCAC 1 cut(s) 657
VpaK11BI GGWCC 1 cut(s) 620
XapI RAATTY 1 cut(s) 320
XceI RCATGY 3 cut(s) 648, 899, 1139
XmiI GTMKAC 3 cut(s) 180, 535, 785
XmnI GAANNNNTTC 1 cut(s) 1124
XspI CTAG 4 cut(s) 735, 1004, 1014, 1154
Zsp2I ATGCAT 3 cut(s) 388, 961, 1384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.