MAP kinase kinase kinase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclI | AACGTT | 1 cut(s) | 33 |
| AdeI | CACNNNGTG | 1 cut(s) | 495 |
| AfaI | GTAC | 2 cut(s) | 95, 430 |
| AflIII | ACRYGT | 1 cut(s) | 81 |
| AgsI | TTSAA | 1 cut(s) | 128 |
| Alw26I | GTCTC | 2 cut(s) | 197, 501 |
| AsuHPI | GGTGA | 1 cut(s) | 362 |
| BccI | CCATC | 1 cut(s) | 376 |
| BcoDI | GTCTC | 2 cut(s) | 197, 501 |
| BfaI | CTAG | 1 cut(s) | 113 |
| BmcAI | AGTACT | 1 cut(s) | 95 |
| BmiI | GGNNCC | 1 cut(s) | 554 |
| BpuEI | CTTGAG | 2 cut(s) | 179, 458 |
| BsaI | GGTCTC | 2 cut(s) | 197, 501 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 444, 474 |
| BseRI | GAGGAG | 2 cut(s) | 178, 590 |
| BsmAI | GTCTC | 2 cut(s) | 197, 501 |
| Bso31I | GGTCTC | 2 cut(s) | 197, 501 |
| Bsp143I | GATC | 2 cut(s) | 216, 303 |
| BspHI | TCATGA | 1 cut(s) | 205 |
| BspLI | GGNNCC | 1 cut(s) | 554 |
| BspTNI | GGTCTC | 2 cut(s) | 197, 501 |
| BssMI | GATC | 2 cut(s) | 216, 303 |
| Bst4CI | ACNGT | 2 cut(s) | 98, 433 |
| Bst6I | CTCTTC | 2 cut(s) | 204, 567 |
| BstDEI | CTNAG | 2 cut(s) | 300, 516 |
| BstKTI | GATC | 2 cut(s) | 219, 306 |
| BstMAI | GTCTC | 2 cut(s) | 197, 501 |
| BstMBI | GATC | 2 cut(s) | 216, 303 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 305 |
| CciI | TCATGA | 1 cut(s) | 205 |
| Csp6I | GTAC | 2 cut(s) | 94, 429 |
| CviAII | CATG | 5 cut(s) | 38, 206, 367, 388, 548 |
| CviJI | RGCY | 2 cut(s) | 419, 426 |
| CviKI_1 | RGCY | 2 cut(s) | 419, 426 |
| CviQI | GTAC | 2 cut(s) | 94, 429 |
| DdeI | CTNAG | 2 cut(s) | 300, 516 |
| DpnI | GATC | 2 cut(s) | 218, 305 |
| DpnII | GATC | 2 cut(s) | 216, 303 |
| DraI | TTTAAA | 1 cut(s) | 30 |
| DraIII | CACNNNGTG | 1 cut(s) | 495 |
| Eam1104I | CTCTTC | 2 cut(s) | 204, 567 |
| EarI | CTCTTC | 2 cut(s) | 204, 567 |
| Eco31I | GGTCTC | 2 cut(s) | 197, 501 |
| FaeI | CATG | 5 cut(s) | 41, 209, 370, 391, 551 |
| FatI | CATG | 5 cut(s) | 37, 205, 366, 387, 547 |
| FspBI | CTAG | 1 cut(s) | 113 |
| Hin1II | CATG | 5 cut(s) | 41, 209, 370, 391, 551 |
| HincII | GTYRAC | 1 cut(s) | 447 |
| HindII | GTYRAC | 1 cut(s) | 447 |
| HphI | GGTGA | 1 cut(s) | 362 |
| Hpy166II | GTNNAC | 1 cut(s) | 447 |
| Hpy188III | TCNNGA | 2 cut(s) | 102, 206 |
| Hpy8I | GTNNAC | 1 cut(s) | 447 |
| HpyAV | CCTTC | 1 cut(s) | 347 |
| HpyCH4III | ACNGT | 2 cut(s) | 98, 433 |
| HpyCH4IV | ACGT | 2 cut(s) | 33, 83 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 305 |
| HpyF3I | CTNAG | 2 cut(s) | 300, 516 |
| HpySE526I | ACGT | 2 cut(s) | 33, 83 |
| Hsp92II | CATG | 5 cut(s) | 41, 209, 370, 391, 551 |
| Kzo9I | GATC | 2 cut(s) | 216, 303 |
| LpnPI | CCDG | 4 cut(s) | 87, 294, 435, 525 |
| MaeI | CTAG | 1 cut(s) | 113 |
| MaeII | ACGT | 2 cut(s) | 33, 83 |
| MaeIII | GTNAC | 3 cut(s) | 84, 227, 362 |
| MalI | GATC | 2 cut(s) | 218, 305 |
| MboI | GATC | 2 cut(s) | 216, 303 |
| MboII | GAAGA | 6 cut(s) | 117, 221, 226, 584, 593, 596 |
| MluCI | AATT | 3 cut(s) | 16, 48, 143 |
| MnlI | CCTC | 7 cut(s) | 131, 156, 367, 513, 568, 571, 610 |
| MseI | TTAA | 3 cut(s) | 29, 47, 558 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 305 |
| NdeII | GATC | 2 cut(s) | 216, 303 |
| NlaIII | CATG | 5 cut(s) | 41, 209, 370, 391, 551 |
| NlaIV | GGNNCC | 1 cut(s) | 554 |
| NmuCI | GTSAC | 2 cut(s) | 227, 362 |
| PagI | TCATGA | 1 cut(s) | 205 |
| Psp1406I | AACGTT | 1 cut(s) | 33 |
| PspN4I | GGNNCC | 1 cut(s) | 554 |
| RsaI | GTAC | 2 cut(s) | 95, 430 |
| RsaNI | GTAC | 2 cut(s) | 94, 429 |
| SaqAI | TTAA | 3 cut(s) | 29, 47, 558 |
| Sau3AI | GATC | 2 cut(s) | 216, 303 |
| ScaI | AGTACT | 1 cut(s) | 95 |
| SetI | ASST | 7 cut(s) | 36, 86, 114, 364, 378, 524, 621 |
| SmlI | CTYRAG | 2 cut(s) | 194, 437 |
| SmoI | CTYRAG | 2 cut(s) | 194, 437 |
| Sse9I | AATT | 3 cut(s) | 16, 48, 143 |
| SspMI | CTAG | 1 cut(s) | 113 |
| TaaI | ACNGT | 2 cut(s) | 98, 433 |
| TaiI | ACGT | 2 cut(s) | 36, 86 |
| TasI | AATT | 3 cut(s) | 16, 48, 143 |
| TatI | WGTACW | 1 cut(s) | 93 |
| Tru1I | TTAA | 3 cut(s) | 29, 47, 558 |
| Tru9I | TTAA | 3 cut(s) | 29, 47, 558 |
| TseFI | GTSAC | 2 cut(s) | 227, 362 |
| Tsp45I | GTSAC | 2 cut(s) | 227, 362 |
| TspDTI | ATGAA | 6 cut(s) | 26, 194, 212, 222, 615, 642 |
| TspGWI | ACGGA | 1 cut(s) | 150 |
| XspI | CTAG | 1 cut(s) | 113 |
| ZrmI | AGTACT | 1 cut(s) | 95 |