belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 128 |
| AclWI | GGATC | 3 cut(s) | 398, 411, 941 |
| AcoI | YGGCCR | 1 cut(s) | 370 |
| AcuI | CTGAAG | 1 cut(s) | 1125 |
| AfaI | GTAC | 2 cut(s) | 446, 784 |
| AflIII | ACRYGT | 1 cut(s) | 432 |
| AgsI | TTSAA | 4 cut(s) | 73, 81, 479, 1136 |
| AjiI | CACGTC | 2 cut(s) | 308, 708 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 975, 1007 |
| AluBI | AGCT | 3 cut(s) | 450, 835, 1103 |
| AluI | AGCT | 3 cut(s) | 450, 835, 1103 |
| Alw21I | GWGCWC | 1 cut(s) | 236 |
| Alw26I | GTCTC | 4 cut(s) | 89, 344, 524, 548 |
| AlwI | GGATC | 3 cut(s) | 398, 411, 941 |
| Ama87I | CYCGRG | 1 cut(s) | 413 |
| AoxI | GGCC | 2 cut(s) | 139, 370 |
| ApeKI | GCWGC | 1 cut(s) | 1109 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 202, 327 |
| AspS9I | GGNCC | 1 cut(s) | 139 |
| AsuC2I | CCSGG | 1 cut(s) | 725 |
| AsuHPI | GGTGA | 2 cut(s) | 692, 878 |
| AvaI | CYCGRG | 1 cut(s) | 413 |
| BamHI | GGATCC | 1 cut(s) | 403 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 410, 442 |
| BauI | CACGAG | 1 cut(s) | 374 |
| BbsI | GAAGAC | 4 cut(s) | 9, 258, 860, 1017 |
| Bbv12I | GWGCWC | 1 cut(s) | 236 |
| BbvI | GCAGC | 1 cut(s) | 1121 |
| BccI | CCATC | 4 cut(s) | 130, 730, 958, 1052 |
| BceAI | ACGGC | 1 cut(s) | 385 |
| BcnI | CCSGG | 1 cut(s) | 725 |
| BcoDI | GTCTC | 4 cut(s) | 89, 344, 524, 548 |
| BfaI | CTAG | 2 cut(s) | 464, 1097 |
| BfmI | CTRYAG | 1 cut(s) | 384 |
| BglII | AGATCT | 2 cut(s) | 8, 291 |
| BisI | GCNGC | 1 cut(s) | 1110 |
| BlsI | GCNGC | 1 cut(s) | 1111 |
| Bme1390I | CCNGG | 1 cut(s) | 725 |
| BmeT110I | CYCGRG | 1 cut(s) | 413 |
| BmgBI | CACGTC | 2 cut(s) | 308, 708 |
| BmgT120I | GGNCC | 1 cut(s) | 139 |
| BmiI | GGNNCC | 1 cut(s) | 405 |
| BmrFI | CCNGG | 1 cut(s) | 725 |
| BmsI | GCATC | 1 cut(s) | 106 |
| BpiI | GAAGAC | 4 cut(s) | 9, 258, 860, 1017 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 434, 466 |
| BpmI | CTGGAG | 1 cut(s) | 825 |
| Bpu10I | CCTNAGC | 1 cut(s) | 451 |
| BpuEI | CTTGAG | 2 cut(s) | 530, 812 |
| BpuMI | CCSGG | 1 cut(s) | 725 |
| Bsa29I | ATCGAT | 1 cut(s) | 25 |
| BsaI | GGTCTC | 1 cut(s) | 548 |
| BsaJI | CCNNGG | 1 cut(s) | 723 |
| BsaWI | WCCGGW | 1 cut(s) | 499 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 798, 828 |
| Bse1I | ACTGG | 1 cut(s) | 808 |
| BseCI | ATCGAT | 1 cut(s) | 25 |
| BseDI | CCNNGG | 1 cut(s) | 723 |
| BseGI | GGATG | 2 cut(s) | 118, 1051 |
| BseMII | CTCAG | 2 cut(s) | 465, 644 |
| BseNI | ACTGG | 1 cut(s) | 808 |
| BseRI | GAGGAG | 1 cut(s) | 529 |
| BseXI | GCAGC | 1 cut(s) | 1121 |
| Bsh1285I | CGRYCG | 2 cut(s) | 172, 240 |
| BshFI | GGCC | 2 cut(s) | 141, 372 |
| BshVI | ATCGAT | 1 cut(s) | 25 |
| BsiEI | CGRYCG | 2 cut(s) | 172, 240 |
| BsiHKAI | GWGCWC | 1 cut(s) | 236 |
| BsiHKCI | CYCGRG | 1 cut(s) | 413 |
| BsiSI | CCGG | 2 cut(s) | 500, 725 |
| BslFI | GGGAC | 1 cut(s) | 53 |
| BsmAI | GTCTC | 4 cut(s) | 89, 344, 524, 548 |
| BsmBI | CGTCTC | 2 cut(s) | 89, 344 |
| BsmFI | GGGAC | 1 cut(s) | 53 |
| BsmI | GAATGC | 1 cut(s) | 76 |
| BsnI | GGCC | 2 cut(s) | 141, 372 |
| Bso31I | GGTCTC | 1 cut(s) | 548 |
| BsoBI | CYCGRG | 1 cut(s) | 413 |
| Bsp1286I | GDGCHC | 1 cut(s) | 236 |
| BspANI | GGCC | 2 cut(s) | 141, 372 |
| BspCNI | CTCAG | 2 cut(s) | 464, 643 |
| BspDI | ATCGAT | 1 cut(s) | 25 |
| BspHI | TCATGA | 1 cut(s) | 556 |
| BspLI | GGNNCC | 1 cut(s) | 405 |
| BspPI | GGATC | 3 cut(s) | 398, 411, 941 |
| BspTNI | GGTCTC | 1 cut(s) | 548 |
| BsrI | ACTGG | 1 cut(s) | 808 |
| BssECI | CCNNGG | 1 cut(s) | 723 |
| BssSI | CACGAG | 1 cut(s) | 374 |
| Bst2BI | CACGAG | 1 cut(s) | 374 |
| Bst4CI | ACNGT | 2 cut(s) | 56, 787 |
| Bst6I | CTCTTC | 3 cut(s) | 208, 555, 1035 |
| BstDEI | CTNAG | 4 cut(s) | 223, 451, 630, 954 |
| BstF5I | GGATG | 2 cut(s) | 118, 1051 |
| BstMAI | GTCTC | 4 cut(s) | 89, 344, 524, 548 |
| BstMCI | CGRYCG | 2 cut(s) | 172, 240 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 635, 1109 |
| BstSCI | CCNGG | 1 cut(s) | 723 |
| BstSFI | CTRYAG | 1 cut(s) | 384 |
| BstV1I | GCAGC | 1 cut(s) | 1121 |
| BstV2I | GAAGAC | 4 cut(s) | 9, 258, 860, 1017 |
| BstX2I | RGATCY | 4 cut(s) | 8, 291, 403, 946 |
| BstYI | RGATCY | 4 cut(s) | 8, 291, 403, 946 |
| Bsu15I | ATCGAT | 1 cut(s) | 25 |
| BsuRI | GGCC | 2 cut(s) | 141, 372 |
| BsuTUI | ATCGAT | 1 cut(s) | 25 |
| BtrI | CACGTC | 2 cut(s) | 308, 708 |
| BtsCI | GGATG | 2 cut(s) | 118, 1051 |
| BtsIMutI | CAGTG | 1 cut(s) | 41 |
| CciI | TCATGA | 1 cut(s) | 556 |
| Cfr13I | GGNCC | 1 cut(s) | 139 |
| ClaI | ATCGAT | 1 cut(s) | 25 |
| CseI | GACGC | 1 cut(s) | 291 |
| Csp6I | GTAC | 2 cut(s) | 445, 783 |
| CviAII | CATG | 3 cut(s) | 557, 742, 986 |
| CviQI | GTAC | 2 cut(s) | 445, 783 |
| DdeI | CTNAG | 4 cut(s) | 223, 451, 630, 954 |
| EaeI | YGGCCR | 1 cut(s) | 370 |
| Eam1104I | CTCTTC | 3 cut(s) | 208, 555, 1035 |
| EarI | CTCTTC | 3 cut(s) | 208, 555, 1035 |
| Eco31I | GGTCTC | 1 cut(s) | 548 |
| Eco57I | CTGAAG | 1 cut(s) | 1125 |
| Eco88I | CYCGRG | 1 cut(s) | 413 |
| Esp3I | CGTCTC | 2 cut(s) | 89, 344 |
| FaeI | CATG | 3 cut(s) | 560, 745, 989 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 243, 275 |
| FaqI | GGGAC | 1 cut(s) | 53 |
| FatI | CATG | 3 cut(s) | 556, 741, 985 |
| FblI | GTMKAC | 1 cut(s) | 128 |
| Fnu4HI | GCNGC | 1 cut(s) | 1110 |
| FokI | GGATG | 2 cut(s) | 125, 1058 |
| Fsp4HI | GCNGC | 1 cut(s) | 1110 |
| FspBI | CTAG | 2 cut(s) | 464, 1097 |
| GluI | GCNGC | 1 cut(s) | 1110 |
| GsuI | CTGGAG | 1 cut(s) | 825 |
| HaeIII | GGCC | 2 cut(s) | 141, 372 |
| HapII | CCGG | 2 cut(s) | 500, 725 |
| HgaI | GACGC | 1 cut(s) | 291 |
| Hin1II | CATG | 3 cut(s) | 560, 745, 989 |
| HincII | GTYRAC | 1 cut(s) | 801 |
| HindII | GTYRAC | 1 cut(s) | 801 |
| HindIII | AAGCTT | 1 cut(s) | 833 |
| HinfI | GANTC | 4 cut(s) | 47, 122, 517, 1163 |
| HpaII | CCGG | 2 cut(s) | 500, 725 |
| HphI | GGTGA | 2 cut(s) | 692, 878 |
| Hpy166II | GTNNAC | 3 cut(s) | 129, 220, 801 |
| Hpy188I | TCNGA | 5 cut(s) | 22, 94, 633, 907, 1168 |
| Hpy188III | TCNNGA | 6 cut(s) | 263, 415, 453, 557, 711, 1097 |
| Hpy8I | GTNNAC | 3 cut(s) | 129, 220, 801 |
| Hpy99I | CGWCG | 1 cut(s) | 47 |
| HpyAV | CCTTC | 5 cut(s) | 236, 385, 394, 677, 839 |
| HpyCH4III | ACNGT | 2 cut(s) | 56, 787 |
| HpyCH4IV | ACGT | 3 cut(s) | 307, 434, 707 |
| HpyCH4V | TGCA | 1 cut(s) | 929 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 635, 1109 |
| HpyF3I | CTNAG | 4 cut(s) | 223, 451, 630, 954 |
| HpySE526I | ACGT | 3 cut(s) | 307, 434, 707 |
| Hsp92II | CATG | 3 cut(s) | 560, 745, 989 |
| LpnPI | CCDG | 8 cut(s) | 146, 276, 438, 513, 624, 738, 789, 835 |
| Lsp1109I | GCAGC | 1 cut(s) | 1121 |
| LweI | GCATC | 1 cut(s) | 106 |
| MaeI | CTAG | 2 cut(s) | 464, 1097 |
| MaeII | ACGT | 3 cut(s) | 307, 434, 707 |
| MaeIII | GTNAC | 6 cut(s) | 181, 303, 379, 435, 578, 708 |
| MflI | RGATCY | 4 cut(s) | 8, 291, 403, 946 |
| MhlI | GDGCHC | 1 cut(s) | 236 |
| MluCI | AATT | 2 cut(s) | 494, 895 |
| MlyI | GAGTC | 2 cut(s) | 41, 526 |
| MroXI | GAANNNNTTC | 2 cut(s) | 202, 327 |
| MseI | TTAA | 2 cut(s) | 279, 996 |
| MspI | CCGG | 2 cut(s) | 500, 725 |
| MspR9I | CCNGG | 1 cut(s) | 725 |
| Mva1269I | GAATGC | 1 cut(s) | 76 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 635, 1109 |
| NciI | CCSGG | 1 cut(s) | 725 |
| NlaIII | CATG | 3 cut(s) | 560, 745, 989 |
| NlaIV | GGNNCC | 1 cut(s) | 405 |
| NmeAIII | GCCGAG | 2 cut(s) | 30, 253 |
| NmuCI | GTSAC | 4 cut(s) | 303, 379, 578, 708 |
| PaeR7I | CTCGAG | 1 cut(s) | 413 |
| PagI | TCATGA | 1 cut(s) | 556 |
| PctI | GAATGC | 1 cut(s) | 76 |
| PdmI | GAANNNNTTC | 2 cut(s) | 202, 327 |
| PfeI | GAWTC | 2 cut(s) | 122, 1163 |
| PkrI | GCNGC | 1 cut(s) | 1111 |
| Ple19I | CGATCG | 2 cut(s) | 172, 240 |
| PleI | GAGTC | 2 cut(s) | 41, 525 |
| PpsI | GAGTC | 2 cut(s) | 41, 525 |
| PspN4I | GGNNCC | 1 cut(s) | 405 |
| PspPI | GGNCC | 1 cut(s) | 139 |
| PsuI | RGATCY | 4 cut(s) | 8, 291, 403, 946 |
| PvuI | CGATCG | 2 cut(s) | 172, 240 |
| RsaI | GTAC | 2 cut(s) | 446, 784 |
| RsaNI | GTAC | 2 cut(s) | 445, 783 |
| SaqAI | TTAA | 2 cut(s) | 279, 996 |
| SatI | GCNGC | 1 cut(s) | 1110 |
| Sau96I | GGNCC | 1 cut(s) | 139 |
| SchI | GAGTC | 2 cut(s) | 41, 526 |
| ScrFI | CCNGG | 1 cut(s) | 725 |
| SduI | GDGCHC | 1 cut(s) | 236 |
| SfaNI | GCATC | 1 cut(s) | 106 |
| SfcI | CTRYAG | 1 cut(s) | 384 |
| Sfr274I | CTCGAG | 1 cut(s) | 413 |
| SlaI | CTCGAG | 1 cut(s) | 413 |
| SmlI | CTYRAG | 3 cut(s) | 413, 545, 791 |
| SmoI | CTYRAG | 3 cut(s) | 413, 545, 791 |
| Sse9I | AATT | 2 cut(s) | 494, 895 |
| SspMI | CTAG | 2 cut(s) | 464, 1097 |
| StyD4I | CCNGG | 1 cut(s) | 723 |
| TaaI | ACNGT | 2 cut(s) | 56, 787 |
| TaiI | ACGT | 3 cut(s) | 310, 437, 710 |
| TaqI | TCGA | 6 cut(s) | 25, 146, 240, 414, 981, 1074 |
| TasI | AATT | 2 cut(s) | 494, 895 |
| TatI | WGTACW | 1 cut(s) | 444 |
| TfiI | GAWTC | 2 cut(s) | 122, 1163 |
| Tru1I | TTAA | 2 cut(s) | 279, 996 |
| Tru9I | TTAA | 2 cut(s) | 279, 996 |
| TscAI | CASTG | 1 cut(s) | 41 |
| TseFI | GTSAC | 4 cut(s) | 303, 379, 578, 708 |
| TseI | GCWGC | 1 cut(s) | 1109 |
| Tsp45I | GTSAC | 4 cut(s) | 303, 379, 578, 708 |
| TspDTI | ATGAA | 7 cut(s) | 329, 545, 563, 573, 1092, 1101, 1158 |
| TspGWI | ACGGA | 1 cut(s) | 597 |
| TspRI | CASTG | 1 cut(s) | 41 |
| XbaI | TCTAGA | 1 cut(s) | 1096 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 957 |
| XhoI | CTCGAG | 1 cut(s) | 413 |
| XmiI | GTMKAC | 1 cut(s) | 128 |
| XmnI | GAANNNNTTC | 2 cut(s) | 202, 327 |
| XspI | CTAG | 2 cut(s) | 464, 1097 |