AT3G45670
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0020182
Physical Location & Seq
Forward (+)
16765320 .. 16766459
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G45670.1

Sequence Viewer

Length: 1140 bp
ATGGAGATTGATTCTGAGATTTCTGATTCATCGTCAAGCAACAACTCGATGATCCCTCCTCTTCCTCAAAACCCTATTTTGACTGTGAAACTCTCTCCGATCATTCTCCATGTACGACGTTGCTCGAAGAACATTAAAAAGAAGAAACGTACACGGACGCCTTTGAAGAAGAAGAAGAAGAGTATTAGTAGCAGTGTCGAATCTATAATCGGAGTCTCATTCAAATCCGCCTCTCAGAAACATTCCACCACCGTAACTCGCGACGGAGGAGTTAAAAAGATTTCCTCTTGGGTGAAATCTAGGTTACTTGGAGAAGGGGCTTACGGTTGTGTTTACCTAGCGACAAGTAAAGATGACATATACAAAACAGAGAGAGCGATCAAAAGTGCTGATGTTTTAAAGGCTTGGAGTCTTATGCACGAAGGAAGGATCTTAAGAAGTTTGCAATCTCCGTTCGTGATTCGTTGTTACGGCCATGAAATTGCACGAGAAGGGACTGGTCATCAATATAATTTGATTCTCGAGTACTGCTCTGGACAGTGTCTTGCGGATATGATCGAAGATAATCAAGGAGGGATACCGGAGTTTGATGTCAAGCAGTTCGCTATAGATGTTCTGTCTGGTCTCAGTTACATTCACCGCAGAAACATTATTCATTGTGAAATTAAACCGGACAATCTCTTACTCAGCCCTGTTGATCACCGGTTTAGGTCTAATGGGTTTTTGACAAAGATTGCAGATTTCGGTTTATCAATGGAGAAAGGTTCTAAAGAGTATGGAAACGGACGTGGGCACATGAGAGGGACAACTAGGTACATGGCTCCGGAGTTAATAGGCGGTGGGCTTCTGGATTTTGCTGTTGACATTTGCGCGTTTGGATGTTCTGTTTTGGAGATGCTAACCGGGAAAAGGGTTTGGGGAGAATATGGTGATTTGGCTCATGATGATTGGGTTGATTTGATCGGTCACAGTGATCTCACTCCTCAAATCTCGATTCGGTTATCTGCAGAAGCTCAAGACTTTCTGATGAGGTGTTTGGTTAAGGAGCCTGGTTCGAGGTGGACCATCGGTGAACTCGTGGACCATCCGTTTCTCTGTTCAGATGAAGAGTTTTCTCACAATGGATTTGTTTATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

379

Amino Acids

42.33

Weight (kDa)

8.24

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 98 - 365 1e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 101 - 361 2.3e-30 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020182)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18530 AT3G45670 AT3G45790 AT3G46140 AT3G46160 AT4G04632

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 261, 872
AccIII TCCGGA 1 cut(s) 823
AciI CCGC 4 cut(s) 228, 548, 640, 837
AclWI GGATC 2 cut(s) 46, 437
AcoI YGGCCR 1 cut(s) 472
AcyI GRCGYC 1 cut(s) 158
AfaI GTAC 4 cut(s) 114, 151, 527, 815
AfiI CCNNNNNNNGG 1 cut(s) 909
AflII CTTAAG 1 cut(s) 433
AgeI ACCGGT 1 cut(s) 702
AgsI TTSAA 2 cut(s) 166, 223
AjiI CACGTC 1 cut(s) 788
AjnI CCWGG 1 cut(s) 1048
AluBI AGCT 1 cut(s) 1013
AluI AGCT 1 cut(s) 1013
Alw26I GTCTC 2 cut(s) 220, 629
AlwI GGATC 2 cut(s) 46, 437
Ama87I CYCGRG 1 cut(s) 521
Aor13HI TCCGGA 1 cut(s) 823
AoxI GGCC 1 cut(s) 472
AsiGI ACCGGT 1 cut(s) 702
AspLEI GCGC 1 cut(s) 872
AspS9I GGNCC 2 cut(s) 1062, 1081
AsuC2I CCSGG 1 cut(s) 904
AsuHPI GGTGA 5 cut(s) 304, 629, 692, 941, 1082
AvaI CYCGRG 1 cut(s) 521
AvaII GGWCC 2 cut(s) 1062, 1081
BaeGI GKGCMC 1 cut(s) 795
BaeI ACNNNNGTAYC 2 cut(s) 569, 602
BauI CACGAG 2 cut(s) 486, 1076
BccI CCATC 2 cut(s) 1073, 1092
BceAI ACGGC 1 cut(s) 487
BciT130I CCWGG 1 cut(s) 1050
BciVI GTATCC 1 cut(s) 570
BclI TGATCA 1 cut(s) 697
BcnI CCSGG 1 cut(s) 904
BcoDI GTCTC 2 cut(s) 220, 629
BfaI CTAG 3 cut(s) 300, 338, 810
BfmI CTRYAG 2 cut(s) 606, 1005
BfrI CTTAAG 1 cut(s) 433
BfuI GTATCC 1 cut(s) 570
BmcAI AGTACT 1 cut(s) 527
Bme1390I CCNGG 2 cut(s) 904, 1050
Bme18I GGWCC 2 cut(s) 1062, 1081
BmeT110I CYCGRG 1 cut(s) 521
BmgBI CACGTC 1 cut(s) 788
BmgT120I GGNCC 2 cut(s) 1062, 1081
BmiI GGNNCC 2 cut(s) 822, 1047
BmrFI CCNGG 2 cut(s) 904, 1050
BmsI GCATC 1 cut(s) 885
BplI GAGNNNNNCTC 2 cut(s) 515, 547
BpuEI CTTGAG 1 cut(s) 999
BpuMI CCSGG 1 cut(s) 904
BsaHI GRCGYC 1 cut(s) 158
BsaI GGTCTC 1 cut(s) 629
BsaWI WCCGGW 4 cut(s) 580, 670, 702, 823
Bsc4I CCNNNNNNNGG 1 cut(s) 909
Bse118I RCCGGY 1 cut(s) 702
Bse1I ACTGG 1 cut(s) 502
BseAI TCCGGA 1 cut(s) 823
BseBI CCWGG 1 cut(s) 1050
BseGI GGATG 2 cut(s) 884, 1084
BseLI CCNNNNNNNGG 1 cut(s) 909
BseMII CTCAG 4 cut(s) 6, 248, 640, 700
BseNI ACTGG 1 cut(s) 502
BseRI GAGGAG 3 cut(s) 48, 282, 972
BseSI GKGCMC 1 cut(s) 795
Bsh1236I CGCG 2 cut(s) 261, 872
BshFI GGCC 1 cut(s) 474
BshTI ACCGGT 1 cut(s) 702
BsiHKCI CYCGRG 1 cut(s) 521
BsiSI CCGG 5 cut(s) 581, 671, 703, 824, 903
BslFI GGGAC 2 cut(s) 508, 817
BslI CCNNNNNNNGG 1 cut(s) 909
BsmAI GTCTC 2 cut(s) 220, 629
BsmFI GGGAC 2 cut(s) 508, 817
BsnI GGCC 1 cut(s) 474
Bso31I GGTCTC 1 cut(s) 629
BsoBI CYCGRG 1 cut(s) 521
Bsp1286I GDGCHC 1 cut(s) 795
Bsp13I TCCGGA 1 cut(s) 823
Bsp143I GATC 8 cut(s) 51, 99, 378, 429, 555, 697, 960, 973
Bsp68I TCGCGA 1 cut(s) 261
BspACI CCGC 4 cut(s) 228, 548, 640, 837
BspANI GGCC 1 cut(s) 474
BspCNI CTCAG 4 cut(s) 7, 247, 639, 699
BspEI TCCGGA 1 cut(s) 823
BspFNI CGCG 2 cut(s) 261, 872
BspHI TCATGA 1 cut(s) 940
BspLI GGNNCC 2 cut(s) 822, 1047
BspMAI CTGCAG 1 cut(s) 1009
BspPI GGATC 2 cut(s) 46, 437
BspTI CTTAAG 1 cut(s) 433
BspTNI GGTCTC 1 cut(s) 629
BsrFI RCCGGY 1 cut(s) 702
BsrI ACTGG 1 cut(s) 502
BssAI RCCGGY 1 cut(s) 702
BssMI GATC 8 cut(s) 51, 99, 378, 429, 555, 697, 960, 973
BssNI GRCGYC 1 cut(s) 158
BssSI CACGAG 2 cut(s) 486, 1076
Bst2BI CACGAG 2 cut(s) 486, 1076
Bst2UI CCWGG 1 cut(s) 1050
Bst4CI ACNGT 5 cut(s) 85, 253, 326, 540, 971
Bst6I CTCTTC 3 cut(s) 66, 173, 1101
BstACI GRCGYC 1 cut(s) 158
BstAFI CTTAAG 1 cut(s) 433
BstDEI CTNAG 4 cut(s) 15, 234, 626, 686
BstF5I GGATG 2 cut(s) 884, 1084
BstFNI CGCG 2 cut(s) 261, 872
BstHHI GCGC 1 cut(s) 872
BstKTI GATC 8 cut(s) 54, 102, 381, 432, 558, 700, 963, 976
BstMAI GTCTC 2 cut(s) 220, 629
BstMBI GATC 8 cut(s) 51, 99, 378, 429, 555, 697, 960, 973
BstNI CCWGG 1 cut(s) 1050
BstSCI CCNGG 2 cut(s) 902, 1048
BstSFI CTRYAG 2 cut(s) 606, 1005
BstSLI GKGCMC 1 cut(s) 795
BstUI CGCG 2 cut(s) 261, 872
BstX2I RGATCY 1 cut(s) 429
BstYI RGATCY 1 cut(s) 429
BsuI GTATCC 1 cut(s) 570
BsuRI GGCC 1 cut(s) 474
BtrI CACGTC 1 cut(s) 788
BtsCI GGATG 2 cut(s) 884, 1084
BtsI GCAGTG 1 cut(s) 199
BtsIMutI CAGTG 3 cut(s) 199, 545, 976
BtuMI TCGCGA 1 cut(s) 261
CciI TCATGA 1 cut(s) 940
CfoI GCGC 1 cut(s) 872
Cfr10I RCCGGY 1 cut(s) 702
Cfr13I GGNCC 2 cut(s) 1062, 1081
CseI GACGC 1 cut(s) 166
Csp6I GTAC 4 cut(s) 113, 150, 526, 814
CspAI ACCGGT 1 cut(s) 702
CviAII CATG 5 cut(s) 110, 476, 796, 817, 941
CviJI RGCY 9 cut(s) 320, 404, 474, 690, 821, 844, 938, 1013, 1048
CviKI_1 RGCY 9 cut(s) 320, 404, 474, 690, 821, 844, 938, 1013, 1048
CviQI GTAC 4 cut(s) 113, 150, 526, 814
DdeI CTNAG 4 cut(s) 15, 234, 626, 686
DpnI GATC 8 cut(s) 53, 101, 380, 431, 557, 699, 962, 975
DpnII GATC 8 cut(s) 51, 99, 378, 429, 555, 697, 960, 973
DraI TTTAAA 1 cut(s) 399
EaeI YGGCCR 1 cut(s) 472
Eam1104I CTCTTC 3 cut(s) 66, 173, 1101
EarI CTCTTC 3 cut(s) 66, 173, 1101
EciI GGCGGA 1 cut(s) 217
Eco31I GGTCTC 1 cut(s) 629
Eco47I GGWCC 2 cut(s) 1062, 1081
Eco88I CYCGRG 1 cut(s) 521
EcoRII CCWGG 1 cut(s) 1048
FaeI CATG 5 cut(s) 113, 479, 799, 820, 944
FaqI GGGAC 2 cut(s) 508, 817
FatI CATG 5 cut(s) 109, 475, 795, 816, 940
FbaI TGATCA 1 cut(s) 697
FokI GGATG 2 cut(s) 891, 1071
FspBI CTAG 3 cut(s) 300, 338, 810
GlaI GCGC 1 cut(s) 871
HaeIII GGCC 1 cut(s) 474
HapII CCGG 5 cut(s) 581, 671, 703, 824, 903
HgaI GACGC 1 cut(s) 166
HhaI GCGC 1 cut(s) 872
Hin1I GRCGYC 1 cut(s) 158
Hin1II CATG 5 cut(s) 113, 479, 799, 820, 944
Hin6I GCGC 1 cut(s) 870
HinP1I GCGC 1 cut(s) 870
HincII GTYRAC 1 cut(s) 862
HindII GTYRAC 1 cut(s) 862
HinfI GANTC 8 cut(s) 11, 26, 200, 213, 409, 460, 517, 994
HpaII CCGG 5 cut(s) 581, 671, 703, 824, 903
HphI GGTGA 5 cut(s) 304, 629, 692, 941, 1082
Hpy166II GTNNAC 6 cut(s) 152, 334, 862, 1062, 1073, 1081
Hpy188I TCNGA 7 cut(s) 16, 25, 99, 212, 237, 1026, 1102
Hpy188III TCNNGA 9 cut(s) 260, 457, 521, 534, 824, 848, 941, 991, 1016
Hpy8I GTNNAC 6 cut(s) 152, 334, 862, 1062, 1073, 1081
Hpy99I CGWCG 2 cut(s) 120, 266
HpyAV CCTTC 4 cut(s) 308, 416, 420, 485
HpyCH4III ACNGT 5 cut(s) 85, 253, 326, 540, 971
HpyCH4IV ACGT 3 cut(s) 118, 148, 787
HpyCH4V TGCA 5 cut(s) 418, 445, 485, 737, 1007
HpyF3I CTNAG 4 cut(s) 15, 234, 626, 686
HpySE526I ACGT 3 cut(s) 118, 148, 787
Hsp92I GRCGYC 1 cut(s) 158
Hsp92II CATG 5 cut(s) 113, 479, 799, 820, 944
HspAI GCGC 1 cut(s) 870
Kpn2I TCCGGA 1 cut(s) 823
Ksp22I TGATCA 1 cut(s) 697
Kzo9I GATC 8 cut(s) 51, 99, 378, 429, 555, 697, 960, 973
LmnI GCTCC 2 cut(s) 826, 1045
LweI GCATC 1 cut(s) 885
MaeI CTAG 3 cut(s) 300, 338, 810
MaeII ACGT 3 cut(s) 118, 148, 787
MaeIII GTNAC 5 cut(s) 253, 303, 467, 629, 965
MalI GATC 8 cut(s) 53, 101, 380, 431, 557, 699, 962, 975
MboI GATC 8 cut(s) 51, 99, 378, 429, 555, 697, 960, 973
MflI RGATCY 1 cut(s) 429
MhlI GDGCHC 1 cut(s) 795
MluCI AATT 3 cut(s) 480, 511, 663
MlyI GAGTC 2 cut(s) 222, 418
MroI TCCGGA 1 cut(s) 823
MseI TTAA 7 cut(s) 135, 273, 398, 434, 666, 830, 1041
MspCI CTTAAG 1 cut(s) 433
MspI CCGG 5 cut(s) 581, 671, 703, 824, 903
MspR9I CCNGG 2 cut(s) 904, 1050
MvaI CCWGG 1 cut(s) 1050
MvnI CGCG 2 cut(s) 261, 872
NciI CCSGG 1 cut(s) 904
NdeII GATC 8 cut(s) 51, 99, 378, 429, 555, 697, 960, 973
NlaIII CATG 5 cut(s) 113, 479, 799, 820, 944
NlaIV GGNNCC 2 cut(s) 822, 1047
NmuCI GTSAC 1 cut(s) 965
NruI TCGCGA 1 cut(s) 261
PaeR7I CTCGAG 1 cut(s) 521
PagI TCATGA 1 cut(s) 940
PcsI WCGNNNNNNNCGW 1 cut(s) 1074
PfeI GAWTC 6 cut(s) 11, 26, 200, 460, 517, 994
PflFI GACNNNGTC 1 cut(s) 540
PinAI ACCGGT 1 cut(s) 702
PleI GAGTC 2 cut(s) 221, 417
PpsI GAGTC 2 cut(s) 221, 417
Psp6I CCWGG 1 cut(s) 1048
PspGI CCWGG 1 cut(s) 1048
PspN4I GGNNCC 2 cut(s) 822, 1047
PspPI GGNCC 2 cut(s) 1062, 1081
PstI CTGCAG 1 cut(s) 1009
PsuI RGATCY 1 cut(s) 429
PsyI GACNNNGTC 1 cut(s) 540
RruI TCGCGA 1 cut(s) 261
RsaI GTAC 4 cut(s) 114, 151, 527, 815
RsaNI GTAC 4 cut(s) 113, 150, 526, 814
SaqAI TTAA 7 cut(s) 135, 273, 398, 434, 666, 830, 1041
Sau3AI GATC 8 cut(s) 51, 99, 378, 429, 555, 697, 960, 973
Sau96I GGNCC 2 cut(s) 1062, 1081
ScaI AGTACT 1 cut(s) 527
SchI GAGTC 2 cut(s) 222, 418
ScrFI CCNGG 2 cut(s) 904, 1050
SduI GDGCHC 1 cut(s) 795
SfaNI GCATC 1 cut(s) 885
SfcI CTRYAG 2 cut(s) 606, 1005
Sfr274I CTCGAG 1 cut(s) 521
SinI GGWCC 2 cut(s) 1062, 1081
SlaI CTCGAG 1 cut(s) 521
SmlI CTYRAG 3 cut(s) 433, 521, 1014
SmoI CTYRAG 3 cut(s) 433, 521, 1014
Sse9I AATT 3 cut(s) 480, 511, 663
SsiI CCGC 4 cut(s) 228, 548, 640, 837
SspMI CTAG 3 cut(s) 300, 338, 810
StyD4I CCNGG 2 cut(s) 902, 1048
TaaI ACNGT 5 cut(s) 85, 253, 326, 540, 971
TaiI ACGT 3 cut(s) 121, 151, 790
TaqI TCGA 7 cut(s) 47, 125, 198, 522, 558, 992, 1055
TaqII GACCGA 1 cut(s) 953
TasI AATT 3 cut(s) 480, 511, 663
TatI WGTACW 1 cut(s) 525
TfiI GAWTC 6 cut(s) 11, 26, 200, 460, 517, 994
Tru1I TTAA 7 cut(s) 135, 273, 398, 434, 666, 830, 1041
Tru9I TTAA 7 cut(s) 135, 273, 398, 434, 666, 830, 1041
TscAI CASTG 3 cut(s) 199, 545, 976
TseFI GTSAC 1 cut(s) 965
Tsp45I GTSAC 1 cut(s) 965
TspDTI ATGAA 4 cut(s) 18, 492, 644, 1119
TspGWI ACGGA 5 cut(s) 169, 279, 441, 798, 1077
TspRI CASTG 3 cut(s) 199, 545, 976
Tth111I GACNNNGTC 1 cut(s) 540
Vha464I CTTAAG 1 cut(s) 433
VpaK11BI GGWCC 2 cut(s) 1062, 1081
XhoI CTCGAG 1 cut(s) 521
XspI CTAG 3 cut(s) 300, 338, 810
ZrmI AGTACT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.