MAP kinase kinase kinase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 7 |
| AciI | CCGC | 1 cut(s) | 21 |
| AjnI | CCWGG | 1 cut(s) | 232 |
| AluBI | AGCT | 1 cut(s) | 197 |
| AluI | AGCT | 1 cut(s) | 197 |
| Aor13HI | TCCGGA | 1 cut(s) | 7 |
| AspS9I | GGNCC | 1 cut(s) | 265 |
| AsuHPI | GGTGA | 2 cut(s) | 125, 266 |
| AvaII | GGWCC | 1 cut(s) | 265 |
| BauI | CACGAG | 1 cut(s) | 260 |
| BccI | CCATC | 1 cut(s) | 276 |
| BciT130I | CCWGG | 1 cut(s) | 234 |
| BfmI | CTRYAG | 1 cut(s) | 189 |
| Bme1390I | CCNGG | 1 cut(s) | 234 |
| Bme18I | GGWCC | 1 cut(s) | 265 |
| BmgT120I | GGNCC | 1 cut(s) | 265 |
| BmiI | GGNNCC | 2 cut(s) | 6, 231 |
| BmrFI | CCNGG | 1 cut(s) | 234 |
| BmsI | GCATC | 2 cut(s) | 69, 258 |
| BpuEI | CTTGAG | 1 cut(s) | 183 |
| BsaWI | WCCGGW | 1 cut(s) | 7 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 238, 268 |
| BseAI | TCCGGA | 1 cut(s) | 7 |
| BseBI | CCWGG | 1 cut(s) | 234 |
| BseGI | GGATG | 2 cut(s) | 68, 292 |
| BseRI | GAGGAG | 1 cut(s) | 156 |
| BsiSI | CCGG | 1 cut(s) | 8 |
| Bsp13I | TCCGGA | 1 cut(s) | 7 |
| Bsp143I | GATC | 1 cut(s) | 157 |
| BspACI | CCGC | 1 cut(s) | 21 |
| BspEI | TCCGGA | 1 cut(s) | 7 |
| BspHI | TCATGA | 1 cut(s) | 124 |
| BspLI | GGNNCC | 2 cut(s) | 6, 231 |
| BspMAI | CTGCAG | 1 cut(s) | 193 |
| BssMI | GATC | 1 cut(s) | 157 |
| BssSI | CACGAG | 1 cut(s) | 260 |
| Bst2BI | CACGAG | 1 cut(s) | 260 |
| Bst2UI | CCWGG | 1 cut(s) | 234 |
| Bst4CI | ACNGT | 1 cut(s) | 97 |
| BstF5I | GGATG | 2 cut(s) | 68, 292 |
| BstKTI | GATC | 1 cut(s) | 160 |
| BstMBI | GATC | 1 cut(s) | 157 |
| BstNI | CCWGG | 1 cut(s) | 234 |
| BstSCI | CCNGG | 1 cut(s) | 232 |
| BstSFI | CTRYAG | 1 cut(s) | 189 |
| BtsCI | GGATG | 2 cut(s) | 68, 292 |
| CciI | TCATGA | 1 cut(s) | 124 |
| Cfr13I | GGNCC | 1 cut(s) | 265 |
| CviAII | CATG | 2 cut(s) | 110, 125 |
| CviJI | RGCY | 4 cut(s) | 5, 28, 197, 232 |
| CviKI_1 | RGCY | 4 cut(s) | 5, 28, 197, 232 |
| DpnI | GATC | 1 cut(s) | 159 |
| DpnII | GATC | 1 cut(s) | 157 |
| Eco47I | GGWCC | 1 cut(s) | 265 |
| EcoRII | CCWGG | 1 cut(s) | 232 |
| FaeI | CATG | 2 cut(s) | 113, 128 |
| FaiI | YATR | 4 cut(s) | 111, 126, 153, 318 |
| FatI | CATG | 2 cut(s) | 109, 124 |
| FokI | GGATG | 2 cut(s) | 75, 299 |
| HapII | CCGG | 1 cut(s) | 8 |
| Hin1II | CATG | 2 cut(s) | 113, 128 |
| HincII | GTYRAC | 1 cut(s) | 46 |
| HindII | GTYRAC | 1 cut(s) | 46 |
| HinfI | GANTC | 1 cut(s) | 178 |
| HpaII | CCGG | 1 cut(s) | 8 |
| HphI | GGTGA | 2 cut(s) | 125, 266 |
| Hpy166II | GTNNAC | 3 cut(s) | 46, 257, 265 |
| Hpy188I | TCNGA | 1 cut(s) | 286 |
| Hpy188III | TCNNGA | 4 cut(s) | 8, 125, 175, 200 |
| Hpy8I | GTNNAC | 3 cut(s) | 46, 257, 265 |
| HpyCH4III | ACNGT | 1 cut(s) | 97 |
| HpyCH4V | TGCA | 1 cut(s) | 191 |
| Hsp92II | CATG | 2 cut(s) | 113, 128 |
| Kpn2I | TCCGGA | 1 cut(s) | 7 |
| Kzo9I | GATC | 1 cut(s) | 157 |
| LmnI | GCTCC | 3 cut(s) | 10, 229, 246 |
| LpnPI | CCDG | 4 cut(s) | 21, 72, 219, 246 |
| LweI | GCATC | 2 cut(s) | 69, 258 |
| MalI | GATC | 1 cut(s) | 159 |
| MboI | GATC | 1 cut(s) | 157 |
| MluCI | AATT | 1 cut(s) | 144 |
| MlyI | GAGTC | 1 cut(s) | 172 |
| MnlI | CCTC | 3 cut(s) | 177, 207, 234 |
| MroI | TCCGGA | 1 cut(s) | 7 |
| MseI | TTAA | 3 cut(s) | 14, 143, 225 |
| MspI | CCGG | 1 cut(s) | 8 |
| MspR9I | CCNGG | 1 cut(s) | 234 |
| MvaI | CCWGG | 1 cut(s) | 234 |
| NdeII | GATC | 1 cut(s) | 157 |
| NlaIII | CATG | 2 cut(s) | 113, 128 |
| NlaIV | GGNNCC | 2 cut(s) | 6, 231 |
| PagI | TCATGA | 1 cut(s) | 124 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 258 |
| PleI | GAGTC | 1 cut(s) | 172 |
| PpsI | GAGTC | 1 cut(s) | 172 |
| Psp6I | CCWGG | 1 cut(s) | 232 |
| PspGI | CCWGG | 1 cut(s) | 232 |
| PspN4I | GGNNCC | 2 cut(s) | 6, 231 |
| PspPI | GGNCC | 1 cut(s) | 265 |
| PstI | CTGCAG | 1 cut(s) | 193 |
| SaqAI | TTAA | 3 cut(s) | 14, 143, 225 |
| Sau3AI | GATC | 1 cut(s) | 157 |
| Sau96I | GGNCC | 1 cut(s) | 265 |
| SchI | GAGTC | 1 cut(s) | 172 |
| ScrFI | CCNGG | 1 cut(s) | 234 |
| SetI | ASST | 3 cut(s) | 199, 218, 245 |
| SfaNI | GCATC | 2 cut(s) | 69, 258 |
| SfcI | CTRYAG | 1 cut(s) | 189 |
| SinI | GGWCC | 1 cut(s) | 265 |
| SmlI | CTYRAG | 1 cut(s) | 198 |
| SmoI | CTYRAG | 1 cut(s) | 198 |
| Sse9I | AATT | 1 cut(s) | 144 |
| SsiI | CCGC | 1 cut(s) | 21 |
| StyD4I | CCNGG | 1 cut(s) | 232 |
| TaaI | ACNGT | 1 cut(s) | 97 |
| TaqI | TCGA | 2 cut(s) | 176, 239 |
| TasI | AATT | 1 cut(s) | 144 |
| Tru1I | TTAA | 3 cut(s) | 14, 143, 225 |
| Tru9I | TTAA | 3 cut(s) | 14, 143, 225 |
| TspDTI | ATGAA | 1 cut(s) | 113 |
| VpaK11BI | GGWCC | 1 cut(s) | 265 |