Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007837

Physical Location & Seq

Genomic Coordinates Reverse (-)

8755852 .. 8760298

Gene Structure 4447 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G20300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2235 bp

ATGCGGAACTTTGCGATGCTTCTGCTGTTAATTCTTCTTCTTCACTCTCTCGCCTCGTTTCCTATATGCTTTGCTCGGTTATTTCCGATGAGTTTGCCATTTACCCGATCAAAGGCGCATCAAATGCATTTCTTTCATCCTTATCTTAATCCATCAGTTGCTCCTACACCTTCACCAGCTTTTTCCCCCAACCCAAGTCGCATTCCACCACTAAGGCACAAGGGTCATCACCGTCATCGTCGCTGGCATTTAAGACGCAATGCCACTGCAGTGTCACCTTCGTCACATGACTGTCAGCAGACATGTGTGGAGCCTCTCACTTCAACTCCCTTTGGTTCACCTTGTGGTTGTGTCTTCCCCATGAAAGTTCAGCTTCTACTAAGTGTCGCACCTTTTTCTATTTTCCCCGTGACCAACGAGTTAGAAATTGAGGTTGCTGCTGGAACATACTTGGAACAAAGCCAAGTGAAAATAATGGGTGCCAGTGCCGACAGTGAAAATCAAGGGAAAACAGTGGTGGATATTAACCTTGTTCCACTTGGGGAAAAGTTCGACAACACTACAGCAACACTTATTTATCAGAGATTCCGGCACAAGAAAGTACCTCTAAATGAGACTGTTTTTGGTGATTATGAGGTTACGCACATAAGTTATCCAGGAATTCCTTCTTCTTCGCCAAATGGAGATGTTACTGGAGATGCTCCAGGAGGCCTTCCAATTCCGATCAATGCAACAACTTTTGCCAACAAAAGCCAGGGAATAGGTTTTAGAACGATTGCAATTATTGCGTTGTCAGGTTTTGTCCTCATTCTGGTTTTGGTTGGAGCTATTTCTATAATCGTGAAATGGAAGAAAATTGGGAAGTCATCTAATGCTGTTGGTCCGGCTTTGGCTCCATCGATTAACAAAAGGCCTGGTGCTGGATCTATGTTTTCCAGTAGCGCCAGAAGCTCAGGCTCAGATTCTTTGATGTCTTCCATGGCTACATGTGCTTTATCAGTTAAGACCTTCACACTCTCCGAGCTTGAGAAGGCTACTGATAGATTCAGTGCCAAGAGGGTTTTGGGCGAGGGCGGATTTGGTCGTGTTTATCAGGGGAGTATGGAAGATGGAACTGAGGTTGCAGTGAAACTGCTAACTAGGGACAATCAGAATCGAGACCGTGAATTTATTGCCGAAGTCGAGATGCTAAGCCGTTTGCACCACCGCAATCTTGTGAAACTGATTGGAATATGCATTGAAGGTCGTACACGTTGCTTGATTTATGAGCTCGTCCACAATGGGAGTGTTGAGTCCCACTTACACGAAGGAACGCTTGATTGGGATGCGCGGTTGAAGATTGCACTTGGAGCAGCGAGAGGACTGGCTTATCTCCATGAAGATTCAAATCCCCGAGTAATCCACCGTGATTTCAAGGCTAGCAATGTTCTCCTAGAAGATGACTTTACCCCAAAAGTCTCAGACTTTGGACTGGCAAGAGAAGCAACCGAAGGAAGTCAACATATTTCAACTCGAGTCATGGGGACCTTTGGGTACGTGGCTCCTGAGTATGCAATGACGGGGCATCTCCTTGTTAAAAGCGATGTCTATAGTTATGGTGTAGTTCTACTAGAGCTTCTAACCGGTAGAAGACCAGTAGACATGTCTCAACCTTCGGGAGAGGAAAACCTTGTGACATGGGCGAGACCCTTACTAGCCAACAGAGAGGGCCTGGAGCAACTGGTGGACCCTGCATTGGCTGGAACCTACAACTTTGATGACATGGCGAAAGTGGCTGCGATTGCCTCCATGTGCGTCCACCAAGAGGTCTCACACAGACCATTCATGGGTGAAGTTGTGCAGGCACTAAAACTTATATACAACGATGCAGATGAGACCTGTGGTGATTATTGCAGCCAAAAGGACTCATCAGTACCAGACTCTGCTGACTTCAAAGGCGATCTGGCTCCTTCGGATAGCAGCTGGTGGAATTTGACACCTCGGTTAAGGTATGGTCAAGCTTCGTCGTTCATAACAATGGATTATAGCTCAGGACCACTAGAGGACATGGAGAACCGGCCTCACTCTGCCTCTAGCATTCCAAGAGTAGGAGGTCTTATTCTACCAAACAGATCAGGTCCGTTAAGACCCATGCGTAGTAGAAGAAACTTCTTTAGACTGAGAGGAAGCATGAGCGAACACGGTGGACCGTCGTCGTCTAGACATCTCTGGTCCGGCAATGGTGACTGGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010065 GO:0010068 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0042335 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

744

Amino Acids

81.63

Weight (kDa)

8.2

Isoelectric Point (pI)

45.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALE2_N	PF23180	104 - 222	1.1e-54	Receptor-like PK ALE2 N-terminal domain
Pkinase	PF00069	350 - 612	2.4e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	351 - 616	5.2e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007837)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20300
fragaria_vesca	FvH4_2g20190 FvH4_2g20190 FvH4_2g20190 FvH4_2g20190 FvH4_2g20190 FvH4_2g20190
malus_domestica	MD05G1185400.v1.1 MD10G1173600.v1.1
prunus_persica	Prupe.8G212200_v2.0.a1 Prupe.8G212200_v2.0.a1
pyrus_communis	pycom05g17020 pycom10g14970
rosa_chinensis	RchiOBHm_Chr6g0285961
rosa_laevigata	RLG00000012594
rosa_multiflora	Rmu_sc0004808.1_g000010 Rmu_sc0005513.1_g000001
rosa_roxburghii	Rroxscaffold_7G00182570
rosa_rugosa	Rorug06G0177700
rosa_samantha	Rh6AG289800 Rh6BG291700 Rh6CG292700 Rh6DG284800
rosa_wichuraiana	Rw6G024970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	479
AccI	GTMKAC	1 cut(s)	1638
AccII	CGCG	1 cut(s)	1330
AciI	CCGC	4 cut(s)	4, 1074, 1207, 1330
AclWI	GGATC	1 cut(s)	931
AcsI	RAATTY	3 cut(s)	660, 1166, 1969
AdeI	CACNNNGTG	1 cut(s)	344
AfaI	GTAC	4 cut(s)	603, 1249, 1535, 1914
AfiI	CCNNNNNNNGG	7 cut(s)	112, 811, 920, 1735, 1804, 1826, 2087
AflIII	ACRYGT	4 cut(s)	302, 986, 1250, 1641
AgeI	ACCGGT	1 cut(s)	1622
AgsI	TTSAA	7 cut(s)	324, 1241, 1336, 1386, 1414, 1509, 1933
AjnI	CCWGG	5 cut(s)	655, 703, 753, 913, 1710
AjuI	GAANNNNNNNTTGG	2 cut(s)	1303, 1335
AleI	CACNNNNGTG	1 cut(s)	269
Alw21I	GWGCWC	1 cut(s)	1272
Alw26I	GTCTC	7 cut(s)	608, 1152, 1462, 1650, 1678, 1813, 1868
AlwI	GGATC	1 cut(s)	931
AlwNI	CAGNNNCTG	1 cut(s)	1922
Ama87I	CYCGRG	2 cut(s)	1392, 1512
AoxI	GGCC	4 cut(s)	709, 911, 1708, 2057
ApeKI	GCWGC	5 cut(s)	437, 1352, 1775, 1893, 1959
ApoI	RAATTY	3 cut(s)	660, 1166, 1969
AsiGI	ACCGGT	1 cut(s)	1622
Asp700I	GAANNNNTTC	1 cut(s)	664
AspLEI	GCGC	3 cut(s)	118, 944, 1330
AspS9I	GGNCC	8 cut(s)	881, 1524, 1708, 1726, 2033, 2117, 2186, 2211
AsuHPI	GGTGA	8 cut(s)	165, 221, 267, 330, 638, 1841, 1895, 2234
AsuNHI	GCTAGC	1 cut(s)	1418
AvaI	CYCGRG	2 cut(s)	1392, 1512
AvaII	GGWCC	7 cut(s)	881, 1524, 1726, 2033, 2117, 2186, 2211
BanI	GGYRCC	1 cut(s)	479
BanII	GRGCYC	1 cut(s)	1272
BbsI	GAAGAC	3 cut(s)	346, 966, 1636
Bbv12I	GWGCWC	1 cut(s)	1272
BbvI	GCAGC	5 cut(s)	424, 1364, 1762, 1905, 1971
BccI	CCATC	3 cut(s)	160, 904, 1103
BceAI	ACGGC	1 cut(s)	1179
BcgI	CGANNNNNNTGC	4 cut(s)	4, 38, 76, 110
BciT130I	CCWGG	5 cut(s)	657, 705, 755, 915, 1712
BcoDI	GTCTC	7 cut(s)	608, 1152, 1462, 1650, 1678, 1813, 1868
BfaI	CTAG	8 cut(s)	1140, 1419, 1433, 1610, 1694, 2039, 2073, 2199
BfmI	CTRYAG	3 cut(s)	267, 561, 1588
BfoI	RGCGCY	1 cut(s)	945
BisI	GCNGC	5 cut(s)	438, 1353, 1776, 1894, 1960
BlpI	GCTNAGC	1 cut(s)	1190
BlsI	GCNGC	5 cut(s)	439, 1354, 1777, 1895, 1961
Bme1390I	CCNGG	5 cut(s)	657, 705, 755, 915, 1712
Bme18I	GGWCC	7 cut(s)	881, 1524, 1726, 2033, 2117, 2186, 2211
BmeT110I	CYCGRG	2 cut(s)	1392, 1512
BmgT120I	GGNCC	8 cut(s)	881, 1524, 1708, 1726, 2033, 2117, 2186, 2211
BmiI	GGNNCC	8 cut(s)	312, 481, 894, 1525, 1542, 1728, 1744, 1947
BmrFI	CCNGG	5 cut(s)	657, 705, 755, 915, 1712
BmsI	GCATC	7 cut(s)	6, 127, 688, 1176, 1315, 1573, 1855
BmtI	GCTAGC	1 cut(s)	1422
BoxI	GACNNNNGTC	1 cut(s)	2191
BpiI	GAAGAC	3 cut(s)	346, 966, 1636
BpmI	CTGGAG	3 cut(s)	687, 714, 1733
Bpu10I	CCTNAGC	2 cut(s)	952, 2029
Bpu1102I	GCTNAGC	1 cut(s)	1190
BpuEI	CTTGAG	1 cut(s)	1046
Bsa29I	ATCGAT	1 cut(s)	899
BsaAI	YACGTR	1 cut(s)	1537
BsaBI	GATNNNNATC	1 cut(s)	1386
BsaI	GGTCTC	4 cut(s)	1152, 1678, 1813, 1868
BsaJI	CCNNGG	3 cut(s)	754, 978, 1979
BsaWI	WCCGGW	1 cut(s)	1622
BsaXI	ACNNNNNCTCC	2 cut(s)	310, 340
Bsc4I	CCNNNNNNNGG	7 cut(s)	112, 811, 920, 1735, 1804, 1826, 2087
Bse118I	RCCGGY	2 cut(s)	1622, 2055
Bse1I	ACTGG	8 cut(s)	483, 697, 936, 1368, 1476, 1634, 1725, 2231
Bse3DI	GCAATG	4 cut(s)	265, 1429, 1560, 2224
Bse8I	GATNNNNATC	1 cut(s)	1386
BseBI	CCWGG	5 cut(s)	657, 705, 755, 915, 1712
BseCI	ATCGAT	1 cut(s)	899
BseDI	CCNNGG	3 cut(s)	754, 978, 1979
BseGI	GGATG	2 cut(s)	136, 1330
BseJI	GATNNNNATC	1 cut(s)	1386
BseLI	CCNNNNNNNGG	7 cut(s)	112, 811, 920, 1735, 1804, 1826, 2087
BseMI	GCAATG	4 cut(s)	265, 1429, 1560, 2224
BseMII	CTCAG	7 cut(s)	966, 972, 1107, 1473, 1536, 2043, 2150
BseNI	ACTGG	8 cut(s)	483, 697, 936, 1368, 1476, 1634, 1725, 2231
BseXI	GCAGC	5 cut(s)	424, 1364, 1762, 1905, 1971
BsgI	GTGCAG	1 cut(s)	1859
Bsh1236I	CGCG	1 cut(s)	1330
BshFI	GGCC	4 cut(s)	711, 913, 1710, 2059
BshNI	GGYRCC	1 cut(s)	479
BshTI	ACCGGT	1 cut(s)	1622
BshVI	ATCGAT	1 cut(s)	899
BsiHKAI	GWGCWC	1 cut(s)	1272
BsiHKCI	CYCGRG	2 cut(s)	1392, 1512
BsiSI	CCGG	5 cut(s)	589, 884, 1623, 2056, 2214
BslFI	GGGAC	3 cut(s)	1157, 1279, 1537
BslI	CCNNNNNNNGG	7 cut(s)	112, 811, 920, 1735, 1804, 1826, 2087
BsmAI	GTCTC	7 cut(s)	608, 1152, 1462, 1650, 1678, 1813, 1868
BsmFI	GGGAC	3 cut(s)	1157, 1279, 1537
BsmI	GAATGC	2 cut(s)	201, 2076
BsnI	GGCC	4 cut(s)	711, 913, 1710, 2059
Bso31I	GGTCTC	4 cut(s)	1152, 1678, 1813, 1868
BsoBI	CYCGRG	2 cut(s)	1392, 1512
Bsp1286I	GDGCHC	1 cut(s)	1272
Bsp143I	GATC	5 cut(s)	107, 723, 923, 1939, 2111
Bsp1720I	GCTNAGC	1 cut(s)	1190
Bsp19I	CCATGG	1 cut(s)	978
BspACI	CCGC	4 cut(s)	4, 1074, 1207, 1330
BspANI	GGCC	4 cut(s)	711, 913, 1710, 2059
BspCNI	CTCAG	7 cut(s)	965, 971, 1108, 1472, 1537, 2042, 2151
BspDI	ATCGAT	1 cut(s)	899
BspFNI	CGCG	1 cut(s)	1330
BspLI	GGNNCC	8 cut(s)	312, 481, 894, 1525, 1542, 1728, 1744, 1947
BspMAI	CTGCAG	1 cut(s)	271
BspOI	GCTAGC	1 cut(s)	1422
BspPI	GGATC	1 cut(s)	931
BspT107I	GGYRCC	1 cut(s)	479
BspTNI	GGTCTC	4 cut(s)	1152, 1678, 1813, 1868
BsrDI	GCAATG	4 cut(s)	265, 1429, 1560, 2224
BsrFI	RCCGGY	2 cut(s)	1622, 2055
BsrI	ACTGG	8 cut(s)	483, 697, 936, 1368, 1476, 1634, 1725, 2231
BssAI	RCCGGY	2 cut(s)	1622, 2055
BssECI	CCNNGG	3 cut(s)	754, 978, 1979
BssMI	GATC	5 cut(s)	107, 723, 923, 1939, 2111
BssT1I	CCWWGG	1 cut(s)	978
Bst2UI	CCWGG	5 cut(s)	657, 705, 755, 915, 1712
Bst4CI	ACNGT	9 cut(s)	233, 293, 494, 514, 619, 1163, 1406, 2183, 2190
BstAPI	GCANNNNNTGC	2 cut(s)	124, 785
BstBAI	YACGTR	1 cut(s)	1537
BstC8I	GCNNGC	3 cut(s)	245, 1420, 1842
BstDSI	CCRYGG	1 cut(s)	978
BstF5I	GGATG	2 cut(s)	136, 1330
BstFNI	CGCG	1 cut(s)	1330
BstH2I	RGCGCY	1 cut(s)	945
BstHHI	GCGC	3 cut(s)	118, 944, 1330
BstKTI	GATC	5 cut(s)	110, 726, 926, 1942, 2114
BstMAI	GTCTC	7 cut(s)	608, 1152, 1462, 1650, 1678, 1813, 1868
BstMBI	GATC	5 cut(s)	107, 723, 923, 1939, 2111
BstMWI	GCNNNNNNNGC	8 cut(s)	124, 785, 948, 989, 1349, 1481, 1772, 1781
BstNI	CCWGG	5 cut(s)	657, 705, 755, 915, 1712
BstNSI	RCATGY	3 cut(s)	306, 990, 1645
BstPAI	GACNNNNGTC	1 cut(s)	2191
BstSCI	CCNGG	5 cut(s)	655, 703, 753, 913, 1710
BstSFI	CTRYAG	3 cut(s)	267, 561, 1588
BstUI	CGCG	1 cut(s)	1330
BstV1I	GCAGC	5 cut(s)	424, 1364, 1762, 1905, 1971
BstV2I	GAAGAC	3 cut(s)	346, 966, 1636
BstX2I	RGATCY	1 cut(s)	923
BstYI	RGATCY	1 cut(s)	923
Bsu15I	ATCGAT	1 cut(s)	899
BsuRI	GGCC	4 cut(s)	711, 913, 1710, 2059
BsuTUI	ATCGAT	1 cut(s)	899
BtgI	CCRYGG	1 cut(s)	978
BtgZI	GCGATG	2 cut(s)	29, 1596
BtsCI	GGATG	2 cut(s)	136, 1330
BtsI	GCAGTG	3 cut(s)	264, 276, 1131
BtsIMutI	CAGTG	7 cut(s)	264, 276, 490, 499, 519, 1054, 1131
Cac8I	GCNNGC	3 cut(s)	245, 1420, 1842
CaiI	CAGNNNCTG	1 cut(s)	1922
CfoI	GCGC	3 cut(s)	118, 944, 1330
Cfr10I	RCCGGY	2 cut(s)	1622, 2055
Cfr13I	GGNCC	8 cut(s)	881, 1524, 1708, 1726, 2033, 2117, 2186, 2211
ClaI	ATCGAT	1 cut(s)	899
CseI	GACGC	2 cut(s)	264, 1783
Csp6I	GTAC	4 cut(s)	602, 1248, 1534, 1913
CspAI	ACCGGT	1 cut(s)	1622
CviQI	GTAC	4 cut(s)	602, 1248, 1534, 1913
DpnI	GATC	5 cut(s)	109, 725, 925, 1941, 2113
DpnII	GATC	5 cut(s)	107, 723, 923, 1939, 2111
DraIII	CACNNNGTG	1 cut(s)	344
EciI	GGCGGA	1 cut(s)	1089
Ecl136II	GAGCTC	1 cut(s)	1270
Eco130I	CCWWGG	1 cut(s)	978
Eco147I	AGGCCT	2 cut(s)	711, 913
Eco24I	GRGCYC	1 cut(s)	1272
Eco31I	GGTCTC	4 cut(s)	1152, 1678, 1813, 1868
Eco47I	GGWCC	7 cut(s)	881, 1524, 1726, 2033, 2117, 2186, 2211
Eco53kI	GAGCTC	1 cut(s)	1270
Eco88I	CYCGRG	2 cut(s)	1392, 1512
EcoICRI	GAGCTC	1 cut(s)	1270
EcoO109I	RGGNCCY	2 cut(s)	1524, 1708
EcoRI	GAATTC	1 cut(s)	660
EcoRII	CCWGG	5 cut(s)	655, 703, 753, 913, 1710
EcoT14I	CCWWGG	1 cut(s)	978
EcoT22I	ATGCAT	2 cut(s)	129, 1238
EcoT38I	GRGCYC	1 cut(s)	1272
ErhI	CCWWGG	1 cut(s)	978
FalI	AAGNNNNNCTT	4 cut(s)	357, 389, 1299, 1331
FaqI	GGGAC	3 cut(s)	1157, 1279, 1537
FblI	GTMKAC	1 cut(s)	1638
Fnu4HI	GCNGC	5 cut(s)	438, 1353, 1776, 1894, 1960
FokI	GGATG	2 cut(s)	123, 1337
FriOI	GRGCYC	1 cut(s)	1272
Fsp4HI	GCNGC	5 cut(s)	438, 1353, 1776, 1894, 1960
FspBI	CTAG	8 cut(s)	1140, 1419, 1433, 1610, 1694, 2039, 2073, 2199
GlaI	GCGC	3 cut(s)	117, 943, 1329
GluI	GCNGC	5 cut(s)	438, 1353, 1776, 1894, 1960
GsuI	CTGGAG	3 cut(s)	687, 714, 1733
HaeII	RGCGCY	1 cut(s)	945
HaeIII	GGCC	4 cut(s)	711, 913, 1710, 2059
HapII	CCGG	5 cut(s)	589, 884, 1623, 2056, 2214
HgaI	GACGC	2 cut(s)	264, 1783
HhaI	GCGC	3 cut(s)	118, 944, 1330
Hin6I	GCGC	3 cut(s)	116, 942, 1328
HinP1I	GCGC	3 cut(s)	116, 942, 1328
HincII	GTYRAC	1 cut(s)	1499
HindII	GTYRAC	1 cut(s)	1499
HindIII	AAGCTT	1 cut(s)	1998
HinfI	GANTC	9 cut(s)	585, 962, 1044, 1153, 1292, 1382, 1515, 1904, 1919
HpaII	CCGG	5 cut(s)	589, 884, 1623, 2056, 2214
HphI	GGTGA	8 cut(s)	165, 221, 267, 330, 638, 1841, 1895, 2234
Hpy166II	GTNNAC	8 cut(s)	338, 1250, 1276, 1499, 1639, 1726, 1798, 2186
Hpy188I	TCNGA	9 cut(s)	87, 582, 723, 961, 1021, 1152, 1462, 1954, 2234
Hpy188III	TCNNGA	7 cut(s)	841, 1157, 1183, 1544, 1656, 2031, 2199
Hpy8I	GTNNAC	8 cut(s)	338, 1250, 1276, 1499, 1639, 1726, 1798, 2186
Hpy99I	CGWCG	4 cut(s)	243, 2008, 2194, 2197
HpyCH4III	ACNGT	9 cut(s)	233, 293, 494, 514, 619, 1163, 1406, 2183, 2190
HpyCH4IV	ACGT	2 cut(s)	1252, 1536
HpyF10VI	GCNNNNNNNGC	8 cut(s)	124, 785, 948, 989, 1349, 1481, 1772, 1781
HpySE526I	ACGT	2 cut(s)	1252, 1536
HspAI	GCGC	3 cut(s)	116, 942, 1328
Kzo9I	GATC	5 cut(s)	107, 723, 923, 1939, 2111
LmnI	GCTCC	9 cut(s)	166, 310, 706, 824, 898, 1349, 1546, 1714, 1951
Lsp1109I	GCAGC	5 cut(s)	424, 1364, 1762, 1905, 1971
LweI	GCATC	7 cut(s)	6, 127, 688, 1176, 1315, 1573, 1855
MaeI	CTAG	8 cut(s)	1140, 1419, 1433, 1610, 1694, 2039, 2073, 2199
MaeII	ACGT	2 cut(s)	1252, 1536
MaeIII	GTNAC	7 cut(s)	273, 282, 409, 637, 688, 1672, 2222
MalI	GATC	5 cut(s)	109, 725, 925, 1941, 2113
MboI	GATC	5 cut(s)	107, 723, 923, 1939, 2111
MflI	RGATCY	1 cut(s)	923
MhlI	GDGCHC	1 cut(s)	1272
MluCI	AATT	8 cut(s)	30, 426, 660, 717, 780, 855, 1166, 1969
MlyI	GAGTC	4 cut(s)	1301, 1524, 1898, 1913
MmeI	TCCRAC	1 cut(s)	802
Mph1103I	ATGCAT	2 cut(s)	129, 1238
MroXI	GAANNNNTTC	1 cut(s)	664
MseI	TTAA	9 cut(s)	29, 147, 251, 525, 903, 1002, 1575, 1985, 2123
MslI	CAYNNNNRTG	2 cut(s)	269, 2015
MspA1I	CMGCKG	1 cut(s)	1962
MspI	CCGG	5 cut(s)	589, 884, 1623, 2056, 2214
MspR9I	CCNGG	5 cut(s)	657, 705, 755, 915, 1712
Mva1269I	GAATGC	2 cut(s)	201, 2076
MvaI	CCWGG	5 cut(s)	657, 705, 755, 915, 1712
MvnI	CGCG	1 cut(s)	1330
MwoI	GCNNNNNNNGC	8 cut(s)	124, 785, 948, 989, 1349, 1481, 1772, 1781
NcoI	CCATGG	1 cut(s)	978
NdeII	GATC	5 cut(s)	107, 723, 923, 1939, 2111
NheI	GCTAGC	1 cut(s)	1418
NlaIV	GGNNCC	8 cut(s)	312, 481, 894, 1525, 1542, 1728, 1744, 1947
NmuCI	GTSAC	5 cut(s)	273, 282, 409, 1672, 2222
NsiI	ATGCAT	2 cut(s)	129, 1238
NspI	RCATGY	3 cut(s)	306, 990, 1645
OliI	CACNNNNGTG	1 cut(s)	269
PaeR7I	CTCGAG	1 cut(s)	1512
PceI	AGGCCT	2 cut(s)	711, 913
PciI	ACATGT	3 cut(s)	302, 986, 1641
PctI	GAATGC	2 cut(s)	201, 2076
PdmI	GAANNNNTTC	1 cut(s)	664
PfeI	GAWTC	5 cut(s)	585, 962, 1044, 1153, 1382
PfoI	TCCNGGA	2 cut(s)	655, 703
PinAI	ACCGGT	1 cut(s)	1622
PkrI	GCNGC	5 cut(s)	439, 1354, 1777, 1895, 1961
PleI	GAGTC	4 cut(s)	1300, 1523, 1898, 1913
PpsI	GAGTC	4 cut(s)	1300, 1523, 1898, 1913
Ppu21I	YACGTR	1 cut(s)	1537
PpuMI	RGGWCCY	1 cut(s)	1524
PscI	ACATGT	3 cut(s)	302, 986, 1641
PshAI	GACNNNNGTC	1 cut(s)	2191
Psp124BI	GAGCTC	1 cut(s)	1272
Psp5II	RGGWCCY	1 cut(s)	1524
Psp6I	CCWGG	5 cut(s)	655, 703, 753, 913, 1710
PspGI	CCWGG	5 cut(s)	655, 703, 753, 913, 1710
PspN4I	GGNNCC	8 cut(s)	312, 481, 894, 1525, 1542, 1728, 1744, 1947
PspPI	GGNCC	8 cut(s)	881, 1524, 1708, 1726, 2033, 2117, 2186, 2211
PspPPI	RGGWCCY	1 cut(s)	1524
PspXI	VCTCGAGB	1 cut(s)	1512
PstI	CTGCAG	1 cut(s)	271
PstNI	CAGNNNCTG	1 cut(s)	1922
PsuI	RGATCY	1 cut(s)	923
PvuII	CAGCTG	1 cut(s)	1962
RsaI	GTAC	4 cut(s)	603, 1249, 1535, 1914
RsaNI	GTAC	4 cut(s)	602, 1248, 1534, 1913
RseI	CAYNNNNRTG	2 cut(s)	269, 2015
SacI	GAGCTC	1 cut(s)	1272
SaqAI	TTAA	9 cut(s)	29, 147, 251, 525, 903, 1002, 1575, 1985, 2123
SatI	GCNGC	5 cut(s)	438, 1353, 1776, 1894, 1960
Sau3AI	GATC	5 cut(s)	107, 723, 923, 1939, 2111
Sau96I	GGNCC	8 cut(s)	881, 1524, 1708, 1726, 2033, 2117, 2186, 2211
SchI	GAGTC	4 cut(s)	1301, 1524, 1898, 1913
ScrFI	CCNGG	5 cut(s)	657, 705, 755, 915, 1712
SduI	GDGCHC	1 cut(s)	1272
SfaNI	GCATC	7 cut(s)	6, 127, 688, 1176, 1315, 1573, 1855
SfcI	CTRYAG	3 cut(s)	267, 561, 1588
Sfr274I	CTCGAG	1 cut(s)	1512
SinI	GGWCC	7 cut(s)	881, 1524, 1726, 2033, 2117, 2186, 2211
SlaI	CTCGAG	1 cut(s)	1512
SmiMI	CAYNNNNRTG	2 cut(s)	269, 2015
SmlI	CTYRAG	2 cut(s)	1025, 1512
SmoI	CTYRAG	2 cut(s)	1025, 1512
Sse9I	AATT	8 cut(s)	30, 426, 660, 717, 780, 855, 1166, 1969
SseBI	AGGCCT	2 cut(s)	711, 913
SsiI	CCGC	4 cut(s)	4, 1074, 1207, 1330
SspMI	CTAG	8 cut(s)	1140, 1419, 1433, 1610, 1694, 2039, 2073, 2199
SstI	GAGCTC	1 cut(s)	1272
StuI	AGGCCT	2 cut(s)	711, 913
StyD4I	CCNGG	5 cut(s)	655, 703, 753, 913, 1710
StyI	CCWWGG	1 cut(s)	978
TaaI	ACNGT	9 cut(s)	233, 293, 494, 514, 619, 1163, 1406, 2183, 2190
TaiI	ACGT	2 cut(s)	1255, 1539
TaqI	TCGA	5 cut(s)	552, 899, 1156, 1182, 1513
TasI	AATT	8 cut(s)	30, 426, 660, 717, 780, 855, 1166, 1969
TfiI	GAWTC	5 cut(s)	585, 962, 1044, 1153, 1382
Tru1I	TTAA	9 cut(s)	29, 147, 251, 525, 903, 1002, 1575, 1985, 2123
Tru9I	TTAA	9 cut(s)	29, 147, 251, 525, 903, 1002, 1575, 1985, 2123
TscAI	CASTG	7 cut(s)	271, 276, 490, 499, 519, 1054, 1131
TseFI	GTSAC	5 cut(s)	273, 282, 409, 1672, 2222
TseI	GCWGC	5 cut(s)	437, 1352, 1775, 1893, 1959
Tsp45I	GTSAC	5 cut(s)	273, 282, 409, 1672, 2222
TspDTI	ATGAA	5 cut(s)	125, 377, 1392, 1813, 1999
TspGWI	ACGGA	1 cut(s)	2109
TspRI	CASTG	7 cut(s)	271, 276, 490, 499, 519, 1054, 1131
VpaK11BI	GGWCC	7 cut(s)	881, 1524, 1726, 2033, 2117, 2186, 2211
XapI	RAATTY	3 cut(s)	660, 1166, 1969
XbaI	TCTAGA	1 cut(s)	2198
XceI	RCATGY	3 cut(s)	306, 990, 1645
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1060
XhoI	CTCGAG	1 cut(s)	1512
XmiI	GTMKAC	1 cut(s)	1638
XmnI	GAANNNNTTC	1 cut(s)	664
XspI	CTAG	8 cut(s)	1140, 1419, 1433, 1610, 1694, 2039, 2073, 2199
Zsp2I	ATGCAT	2 cut(s)	129, 1238

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.