Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007837

Physical Location & Seq

Genomic Coordinates Reverse (-)

31403442 .. 31407881

Gene Structure 4440 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1185400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2340 bp

ATGCGAACTCCGATTCTGCTTCTGCTCCTTGTTCTCCTCCTGCTCAGCTTGATTTTCTGCTGCTCAGGCGATTTTTCTCTAAATACTTACTTGTCTCCCTCTCAATTGCCAAACCAACCGTTGTCTATGAAAGATTTCTCAATCGAACGCGCAAGGTCAATTTTGATGAGTCTTCCATTTGCTTCAAAACGATCCAAAACATGGGTGGTCAAGCCTTCTTTGGGAACCCTTGCACCTGCACGTTCCCCAATTCATCTTGGTCCTAGCAGTTCTCCTTCACCACGGCGTGGTCATCATCGTCATCGTCATGTGAGAGTGGAACCCCATGATGTTGCTCCAGCACCATCAAAGGACCCAGGTTGTGATCAAATTTGTGTAGAGCCACTTACTGCATCTCCATTTGGTTCACCTTGTGGTTGTGTGTTTCCCATGAAAGTCAAACTCTTACTGGACATAGCTCCTTACGCTATTTTTCCAGTAATGAGTGAGCTAGAGATTGAGATTGCTGAAGGCACATATCTGACGCAAAGTCAAGTGAAAATAATGGGTGCAAGTGCTGATAGTCAAAATCAAGGAAGAACAGTGGTGGATATTAACTTGGTTCCACTAGGCGAGAGGTTTGATAATACCACTGCGATACTGACATATGATAGATTTCAGCATAAGAAAGTGCCTCTAAATTTGACCCTTTTTGGAAACTATGAAGTGGTATACATTAGTTATCCAGGGATACCTTCTTCACCACCATACGAGATTTACGAAGGGCATGGACCAGCTGGAAGTGCTGGAGGTCTCCCTATCACTGCAGATTTTGGCGGCAAGAACCAGAGGATGAATATTAAAACGATTATGATTATTGCTTTGTCTGCATTTGTACTCCTGGTGGTTCTCCTTGGGGCTATCTGGGTCTTTGTGCAATGGAGGAGATTTGGAAGACCATCTAGCGCTGTCGGTCCTGCATTCACATCTTCTATTCACAAAAGATCTGGTATGGGGTCTATCTTATCAAGTAGTATTGCAAGCTCCACTTCAGTGTCCCTAATGTCCACCATGGCTACTAGTATTCTCTCTGTCAAAACATTTCCACTAGCTGAGCTTGAGAAAGCAACAAATAAGTTCAGTTCCCAGAGGGTTTTGGGAGAAGGGGGATTTGGACGTGTTTACCATGGAATTATGGAAGATGGGACTGAAGTTGCAGTCAAAGTGCTTACAAGGGATAATCTAAATCAGAATGGAGACCGTGAATTCATTGCAGAAGTTGAGATGCTAAGCCGATTGCACCACCGTAATCTTGTGAAGTTGATAGGCATTTGTATTGAAGGGCGGACTCGCAGTTTGGTATATGAGATTGTTCGCAATGGAAGTGTTGAGTCTCACTTGCATGGCGTTGACAAGGTAAATGGACCTCTTGACTGGGAAGCACGGATGAAGATTGCCCTTGGGGCAGCAAGAGGATTAGCATATCTTCATGAAGATTCTAATCCTCGTGTTATTCACCGAGATTTTAAGGCTAGTAATGTTTTGCTGGAAGCCGATTTCACGCCCAAGGTTTCAGATTTTGGGTTGGCTAGGGAAGCAACAGAGGGAAGTCATCACATCTCTACGAGGGTCATGGGAACTTTCGGGTATGTTGCCCCAGAATATGCAATGACAGGGCACCTACTTGTCAAGAGCGATGTTTATAGTTATGGCGTTGTGCTGCTGGAACTTCTCTCCGGAAGAAAACCGGTTGACATGTCCCACCCTCCGGGACAGGAGAATTTAGTAACATGGGCACGACCATTGCTAACGAGCAGAGAAGGTTTGCAGCAGTTGGTGGATCCTGCCTTGGCTGGAACCTATGACTTTGACGACATGGCTAAAGTGGCAGCCATTGCTTCCATGTGCGTTCACCCTGAGGTGACCCGCAGACCTTTTATGGGTGAAGTTGTGCAGGCTCTAAAGCTGATATACAATGATACAGATGAGACTGGTGGGGACTGTTATAGTCAAAAGGAGTCTTCTGTCCCGGACTCTGACTTTAAAGGTGATCTTGCTCCATCTGATAGCAGTTGGTGGAATGCTGGCGGACTCACTCCCCGGTTAAATTATGGGCAAGCATCTTCGTTCATCACAATGGAATATAGTTCTGGACCGCTTGAAGATATGGAAAACAGACCGTTTTCAACTTCAAGTCTGGTAGGGGATGAGATATCTTTACCAATCAGACACGGCAACAGATCAGGGCCATTGAGAACAGTCCGAAGCAAGCCAGCATTCTACAGAATAAGAGGAAGTAGGAGTGAGCACAGTGGACTGCTTCCCAAGCAGCGCGCTTGGAACGATGGCTTCTGGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010065 GO:0010068 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0042335 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

780

Amino Acids

85.41

Weight (kDa)

8.0

Isoelectric Point (pI)

44.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALE2_N	PF23180	127 - 245	6.2e-55	Receptor-like PK ALE2 N-terminal domain
Pkinase	PF00069	374 - 584	1.8e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	376 - 646	2e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007837)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20300
fragaria_vesca	FvH4_2g20190 FvH4_2g20190 FvH4_2g20190 FvH4_2g20190 FvH4_2g20190 FvH4_2g20190
malus_domestica	MD05G1185400.v1.1 MD10G1173600.v1.1
prunus_persica	Prupe.8G212200_v2.0.a1 Prupe.8G212200_v2.0.a1
pyrus_communis	pycom05g17020 pycom10g14970
rosa_chinensis	RchiOBHm_Chr6g0285961
rosa_laevigata	RLG00000012594
rosa_multiflora	Rmu_sc0004808.1_g000010 Rmu_sc0005513.1_g000001
rosa_roxburghii	Rroxscaffold_7G00182570
rosa_rugosa	Rorug06G0177700
rosa_samantha	Rh6AG289800 Rh6BG291700 Rh6CG292700 Rh6DG284800
rosa_wichuraiana	Rw6G024970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	244
Acc36I	ACCTGC	1 cut(s)	244
AccB1I	GGYRCC	1 cut(s)	1656
AccB7I	CCANNNNNTGG	2 cut(s)	201, 287
AccI	GTMKAC	1 cut(s)	711
AccII	CGCG	2 cut(s)	150, 2313
AccIII	TCCGGA	1 cut(s)	1715
AciI	CCGC	5 cut(s)	816, 1324, 1906, 2067, 2135
AclWI	GGATC	3 cut(s)	186, 1814, 1827
AcsI	RAATTY	4 cut(s)	369, 679, 1244, 1759
AcuI	CTGAAG	3 cut(s)	528, 1014, 1209
AdeI	CACNNNGTG	2 cut(s)	287, 413
AfaI	GTAC	1 cut(s)	876
AfeI	AGCGCT	1 cut(s)	946
AfiI	CCNNNNNNNGG	5 cut(s)	201, 221, 287, 1747, 1919
AflIII	ACRYGT	2 cut(s)	1156, 1734
AgeI	ACCGGT	1 cut(s)	1726
AgsI	TTSAA	5 cut(s)	186, 1319, 2141, 2166, 2172
AhdI	GACNNNNNGTC	1 cut(s)	528
AhlI	ACTAGT	1 cut(s)	1058
AjiI	CACGTC	1 cut(s)	1157
AjnI	CCWGG	3 cut(s)	355, 724, 879
AjuI	GAANNNNNNNTTGG	2 cut(s)	1134, 1166
AleI	CACNNNNGTG	1 cut(s)	1031
AluBI	AGCT	8 cut(s)	48, 458, 490, 776, 1023, 1091, 1096, 1945
AluI	AGCT	8 cut(s)	48, 458, 490, 776, 1023, 1091, 1096, 1945
Alw21I	GWGCWC	1 cut(s)	2289
Alw26I	GTCTC	5 cut(s)	99, 797, 1230, 1377, 1961
AlwI	GGATC	3 cut(s)	186, 1814, 1827
Aor13HI	TCCGGA	1 cut(s)	1715
Aor51HI	AGCGCT	1 cut(s)	946
AoxI	GGCC	1 cut(s)	2225
ApeKI	GCWGC	6 cut(s)	60, 1445, 1699, 1807, 1868, 2308
ApoI	RAATTY	4 cut(s)	369, 679, 1244, 1759
ArsI	GACNNNNNNTTYG	2 cut(s)	1318, 1350
AsiGI	ACCGGT	1 cut(s)	1726
Asp700I	GAANNNNTTC	1 cut(s)	134
AspLEI	GCGC	4 cut(s)	152, 947, 2313, 2315
AspS9I	GGNCC	7 cut(s)	260, 352, 770, 953, 1403, 2132, 2225
AsuC2I	CCSGG	3 cut(s)	1749, 2009, 2080
AsuHPI	GGTGA	8 cut(s)	270, 399, 732, 1487, 1883, 1912, 1934, 2039
AvaII	GGWCC	6 cut(s)	260, 352, 770, 953, 1403, 2132
AxyI	CCTNAGG	1 cut(s)	1896
BaeGI	GKGCMC	2 cut(s)	1659, 1777
BamHI	GGATCC	1 cut(s)	1819
BanI	GGYRCC	1 cut(s)	1656
BauI	CACGAG	1 cut(s)	1485
BbsI	GAAGAC	3 cut(s)	164, 940, 1992
Bbv12I	GWGCWC	1 cut(s)	2289
BbvI	GCAGC	6 cut(s)	47, 1457, 1686, 1819, 1880, 2320
BccI	CCATC	5 cut(s)	352, 946, 1175, 2047, 2318
BceAI	ACGGC	2 cut(s)	299, 2227
BciT130I	CCWGG	3 cut(s)	357, 726, 881
BciVI	GTATCC	1 cut(s)	723
BclI	TGATCA	1 cut(s)	364
BcnI	CCSGG	3 cut(s)	1749, 2009, 2080
BcoDI	GTCTC	5 cut(s)	99, 797, 1230, 1377, 1961
BcuI	ACTAGT	1 cut(s)	1058
BfaI	CTAG	8 cut(s)	264, 491, 608, 942, 1059, 1088, 1512, 1569
BfmI	CTRYAG	2 cut(s)	804, 2260
BfoI	RGCGCY	1 cut(s)	948
BfuAI	ACCTGC	1 cut(s)	244
BfuI	GTATCC	1 cut(s)	723
BglI	GCCNNNNNGGC	1 cut(s)	1442
BglII	AGATCT	1 cut(s)	983
BisI	GCNGC	7 cut(s)	61, 817, 1446, 1700, 1808, 1869, 2309
BlpI	GCTNAGC	3 cut(s)	44, 1092, 1268
BlsI	GCNGC	7 cut(s)	62, 818, 1447, 1701, 1809, 1870, 2310
Bme1390I	CCNGG	6 cut(s)	357, 726, 881, 1749, 2009, 2080
Bme18I	GGWCC	6 cut(s)	260, 352, 770, 953, 1403, 2132
BmeRI	GACNNNNNGTC	1 cut(s)	528
BmgBI	CACGTC	1 cut(s)	1157
BmgT120I	GGNCC	7 cut(s)	260, 352, 770, 953, 1403, 2132, 2225
BmiI	GGNNCC	7 cut(s)	226, 321, 354, 603, 1658, 1821, 1837
BmrFI	CCNGG	6 cut(s)	357, 726, 881, 1749, 2009, 2080
BmrI	ACTGGG	1 cut(s)	1423
BmsI	GCATC	3 cut(s)	401, 1254, 2108
BmuI	ACTGGG	1 cut(s)	1423
BpiI	GAAGAC	3 cut(s)	164, 940, 1992
BpmI	CTGGAG	2 cut(s)	321, 807
Bpu10I	CCTNAGC	1 cut(s)	64
Bpu1102I	GCTNAGC	3 cut(s)	44, 1092, 1268
BpuEI	CTTGAG	1 cut(s)	1118
BpuMI	CCSGG	3 cut(s)	1749, 2009, 2080
BsaBI	GATNNNNATC	1 cut(s)	1479
BsaI	GGTCTC	2 cut(s)	797, 1230
BsaWI	WCCGGW	2 cut(s)	1715, 1726
BsaXI	ACNNNNNCTCC	2 cut(s)	379, 409
Bsc4I	CCNNNNNNNGG	5 cut(s)	201, 221, 287, 1747, 1919
Bse118I	RCCGGY	1 cut(s)	1726
Bse1I	ACTGG	4 cut(s)	453, 476, 1418, 1975
Bse21I	CCTNAGG	1 cut(s)	1896
Bse3DI	GCAATG	6 cut(s)	923, 1248, 1363, 1653, 1781, 1872
Bse8I	GATNNNNATC	1 cut(s)	1479
BseAI	TCCGGA	1 cut(s)	1715
BseBI	CCWGG	3 cut(s)	357, 726, 881
BseGI	GGATG	3 cut(s)	837, 1431, 2191
BseJI	GATNNNNATC	1 cut(s)	1479
BseLI	CCNNNNNNNGG	5 cut(s)	201, 221, 287, 1747, 1919
BseMI	GCAATG	6 cut(s)	923, 1248, 1363, 1653, 1781, 1872
BseMII	CTCAG	4 cut(s)	58, 78, 1083, 1887
BseNI	ACTGG	4 cut(s)	453, 476, 1418, 1975
BsePI	GCGCGC	1 cut(s)	2311
BseRI	GAGGAG	2 cut(s)	26, 937
BseSI	GKGCMC	2 cut(s)	1659, 1777
BseXI	GCAGC	6 cut(s)	47, 1457, 1686, 1819, 1880, 2320
BsgI	GTGCAG	2 cut(s)	222, 1952
Bsh1236I	CGCG	2 cut(s)	150, 2313
BshFI	GGCC	1 cut(s)	2227
BshNI	GGYRCC	1 cut(s)	1656
BshTI	ACCGGT	1 cut(s)	1726
BsiHKAI	GWGCWC	1 cut(s)	2289
BsiSI	CCGG	5 cut(s)	1716, 1727, 1748, 2009, 2080
BslFI	GGGAC	6 cut(s)	1021, 1198, 1723, 1764, 1991, 1991
BslI	CCNNNNNNNGG	5 cut(s)	201, 221, 287, 1747, 1919
BsmAI	GTCTC	5 cut(s)	99, 797, 1230, 1377, 1961
BsmFI	GGGAC	6 cut(s)	1021, 1198, 1723, 1764, 1991, 1991
BsmI	GAATGC	3 cut(s)	959, 2065, 2255
BsnI	GGCC	1 cut(s)	2227
Bso31I	GGTCTC	2 cut(s)	797, 1230
Bsp1286I	GDGCHC	3 cut(s)	1659, 1777, 2289
Bsp13I	TCCGGA	1 cut(s)	1715
Bsp143I	GATC	6 cut(s)	191, 364, 983, 1819, 2029, 2219
Bsp1720I	GCTNAGC	3 cut(s)	44, 1092, 1268
Bsp19I	CCATGG	2 cut(s)	1050, 1165
BspACI	CCGC	5 cut(s)	816, 1324, 1906, 2067, 2135
BspANI	GGCC	1 cut(s)	2227
BspCNI	CTCAG	4 cut(s)	57, 77, 1084, 1888
BspEI	TCCGGA	1 cut(s)	1715
BspFNI	CGCG	2 cut(s)	150, 2313
BspHI	TCATGA	1 cut(s)	1468
BspLI	GGNNCC	7 cut(s)	226, 321, 354, 603, 1658, 1821, 1837
BspMAI	CTGCAG	1 cut(s)	808
BspMI	ACCTGC	1 cut(s)	244
BspPI	GGATC	3 cut(s)	186, 1814, 1827
BspT107I	GGYRCC	1 cut(s)	1656
BspTNI	GGTCTC	2 cut(s)	797, 1230
BsrDI	GCAATG	6 cut(s)	923, 1248, 1363, 1653, 1781, 1872
BsrFI	RCCGGY	1 cut(s)	1726
BsrI	ACTGG	4 cut(s)	453, 476, 1418, 1975
BssAI	RCCGGY	1 cut(s)	1726
BssHII	GCGCGC	1 cut(s)	2311
BssMI	GATC	6 cut(s)	191, 364, 983, 1819, 2029, 2219
BssNAI	GTATAC	1 cut(s)	712
BssSI	CACGAG	1 cut(s)	1485
BssT1I	CCWWGG	6 cut(s)	892, 1050, 1165, 1438, 1545, 1827
Bst1107I	GTATAC	1 cut(s)	712
Bst2BI	CACGAG	1 cut(s)	1485
Bst2UI	CCWGG	3 cut(s)	357, 726, 881
Bst4CI	ACNGT	8 cut(s)	120, 583, 1241, 1286, 1982, 2160, 2239, 2291
BstAPI	GCANNNNNTGC	1 cut(s)	1874
BstC8I	GCNNGC	7 cut(s)	1021, 1935, 2065, 2097, 2249, 2253, 2313
BstDEI	CTNAG	5 cut(s)	44, 64, 1092, 1268, 1896
BstDSI	CCRYGG	3 cut(s)	281, 1050, 1165
BstEII	GGTNACC	1 cut(s)	1900
BstF5I	GGATG	3 cut(s)	837, 1431, 2191
BstFNI	CGCG	2 cut(s)	150, 2313
BstH2I	RGCGCY	1 cut(s)	948
BstHHI	GCGC	4 cut(s)	152, 947, 2313, 2315
BstKTI	GATC	6 cut(s)	194, 367, 986, 1822, 2032, 2222
BstMAI	GTCTC	5 cut(s)	99, 797, 1230, 1377, 1961
BstMBI	GATC	6 cut(s)	191, 364, 983, 1819, 2029, 2219
BstMWI	GCNNNNNNNGC	9 cut(s)	66, 464, 782, 866, 1442, 1574, 1865, 1874, 2305
BstNI	CCWGG	3 cut(s)	357, 726, 881
BstNSI	RCATGY	1 cut(s)	1738
BstPI	GGTNACC	1 cut(s)	1900
BstSCI	CCNGG	6 cut(s)	355, 724, 879, 1747, 2007, 2078
BstSFI	CTRYAG	2 cut(s)	804, 2260
BstSLI	GKGCMC	2 cut(s)	1659, 1777
BstUI	CGCG	2 cut(s)	150, 2313
BstV1I	GCAGC	6 cut(s)	47, 1457, 1686, 1819, 1880, 2320
BstV2I	GAAGAC	3 cut(s)	164, 940, 1992
BstX2I	RGATCY	2 cut(s)	983, 1819
BstYI	RGATCY	2 cut(s)	983, 1819
BstZ17I	GTATAC	1 cut(s)	712
Bsu36I	CCTNAGG	1 cut(s)	1896
BsuI	GTATCC	1 cut(s)	723
BsuRI	GGCC	1 cut(s)	2227
BtgI	CCRYGG	3 cut(s)	281, 1050, 1165
BtgZI	GCGATG	1 cut(s)	1689
BtrI	CACGTC	1 cut(s)	1157
BtsCI	GGATG	3 cut(s)	837, 1431, 2191
BtsI	GCAGTG	2 cut(s)	630, 801
BtsIMutI	CAGTG	5 cut(s)	588, 630, 801, 1038, 2296
BveI	ACCTGC	1 cut(s)	244
Cac8I	GCNNGC	7 cut(s)	1021, 1935, 2065, 2097, 2249, 2253, 2313
CciI	TCATGA	1 cut(s)	1468
CfoI	GCGC	4 cut(s)	152, 947, 2313, 2315
Cfr10I	RCCGGY	1 cut(s)	1726
Cfr13I	GGNCC	7 cut(s)	260, 352, 770, 953, 1403, 2132, 2225
CseI	GACGC	1 cut(s)	532
Csp6I	GTAC	1 cut(s)	875
CspAI	ACCGGT	1 cut(s)	1726
CviQI	GTAC	1 cut(s)	875
DdeI	CTNAG	5 cut(s)	44, 64, 1092, 1268, 1896
DpnI	GATC	6 cut(s)	193, 366, 985, 1821, 2031, 2221
DpnII	GATC	6 cut(s)	191, 364, 983, 1819, 2029, 2219
DraI	TTTAAA	1 cut(s)	2023
DraIII	CACNNNGTG	2 cut(s)	287, 413
DriI	GACNNNNNGTC	1 cut(s)	528
Eam1105I	GACNNNNNGTC	1 cut(s)	528
EciI	GGCGGA	2 cut(s)	1339, 2082
Eco130I	CCWWGG	6 cut(s)	892, 1050, 1165, 1438, 1545, 1827
Eco31I	GGTCTC	2 cut(s)	797, 1230
Eco32I	GATATC	1 cut(s)	2193
Eco47I	GGWCC	6 cut(s)	260, 352, 770, 953, 1403, 2132
Eco47III	AGCGCT	1 cut(s)	946
Eco57I	CTGAAG	3 cut(s)	528, 1014, 1209
Eco81I	CCTNAGG	1 cut(s)	1896
Eco91I	GGTNACC	1 cut(s)	1900
EcoO109I	RGGNCCY	1 cut(s)	352
EcoO65I	GGTNACC	1 cut(s)	1900
EcoRI	GAATTC	1 cut(s)	1244
EcoRII	CCWGG	3 cut(s)	355, 724, 879
EcoRV	GATATC	1 cut(s)	2193
EcoT14I	CCWWGG	6 cut(s)	892, 1050, 1165, 1438, 1545, 1827
ErhI	CCWWGG	6 cut(s)	892, 1050, 1165, 1438, 1545, 1827
FalI	AAGNNNNNCTT	4 cut(s)	1012, 1044, 2016, 2048
FaqI	GGGAC	6 cut(s)	1021, 1198, 1723, 1764, 1991, 1991
FauI	CCCGC	1 cut(s)	1913
FauNDI	CATATG	1 cut(s)	646
FbaI	TGATCA	1 cut(s)	364
FblI	GTMKAC	1 cut(s)	711
Fnu4HI	GCNGC	7 cut(s)	61, 817, 1446, 1700, 1808, 1869, 2309
FokI	GGATG	3 cut(s)	844, 1438, 2198
Fsp4HI	GCNGC	7 cut(s)	61, 817, 1446, 1700, 1808, 1869, 2309
FspBI	CTAG	8 cut(s)	264, 491, 608, 942, 1059, 1088, 1512, 1569
GlaI	GCGC	4 cut(s)	151, 946, 2312, 2314
GluI	GCNGC	7 cut(s)	61, 817, 1446, 1700, 1808, 1869, 2309
GsuI	CTGGAG	2 cut(s)	321, 807
HaeII	RGCGCY	1 cut(s)	948
HaeIII	GGCC	1 cut(s)	2227
HapII	CCGG	5 cut(s)	1716, 1727, 1748, 2009, 2080
HgaI	GACGC	1 cut(s)	532
HhaI	GCGC	4 cut(s)	152, 947, 2313, 2315
Hin6I	GCGC	4 cut(s)	150, 945, 2311, 2313
HinP1I	GCGC	4 cut(s)	150, 945, 2311, 2313
HincII	GTYRAC	2 cut(s)	1390, 1732
HindII	GTYRAC	2 cut(s)	1390, 1732
HinfI	GANTC	8 cut(s)	13, 169, 1327, 1370, 1475, 1997, 2012, 2070
HpaII	CCGG	5 cut(s)	1716, 1727, 1748, 2009, 2080
HphI	GGTGA	8 cut(s)	270, 399, 732, 1487, 1883, 1912, 1934, 2039
Hpy166II	GTNNAC	8 cut(s)	407, 712, 1047, 1162, 1390, 1732, 1891, 2294
Hpy188I	TCNGA	8 cut(s)	12, 522, 1230, 1555, 2017, 2044, 2207, 2243
Hpy188III	TCNNGA	5 cut(s)	1409, 1469, 1669, 1716, 2130
Hpy8I	GTNNAC	8 cut(s)	407, 712, 1047, 1162, 1390, 1732, 1891, 2294
HpyAV	CCTTC	8 cut(s)	225, 285, 503, 744, 755, 1136, 1313, 1793
HpyCH4III	ACNGT	8 cut(s)	120, 583, 1241, 1286, 1982, 2160, 2239, 2291
HpyCH4IV	ACGT	2 cut(s)	241, 1156
HpyF10VI	GCNNNNNNNGC	9 cut(s)	66, 464, 782, 866, 1442, 1574, 1865, 1874, 2305
HpyF3I	CTNAG	5 cut(s)	44, 64, 1092, 1268, 1896
HpySE526I	ACGT	2 cut(s)	241, 1156
HspAI	GCGC	4 cut(s)	150, 945, 2311, 2313
Kpn2I	TCCGGA	1 cut(s)	1715
Ksp22I	TGATCA	1 cut(s)	364
Kzo9I	GATC	6 cut(s)	191, 364, 983, 1819, 2029, 2219
LmnI	GCTCC	5 cut(s)	30, 340, 463, 1028, 2041
Lsp1109I	GCAGC	6 cut(s)	47, 1457, 1686, 1819, 1880, 2320
LweI	GCATC	3 cut(s)	401, 1254, 2108
MaeI	CTAG	8 cut(s)	264, 491, 608, 942, 1059, 1088, 1512, 1569
MaeII	ACGT	2 cut(s)	241, 1156
MaeIII	GTNAC	2 cut(s)	1765, 1900
MalI	GATC	6 cut(s)	193, 366, 985, 1821, 2031, 2221
MboI	GATC	6 cut(s)	191, 364, 983, 1819, 2029, 2219
MfeI	CAATTG	1 cut(s)	104
MflI	RGATCY	2 cut(s)	983, 1819
MhlI	GDGCHC	3 cut(s)	1659, 1777, 2289
MluCI	AATT	9 cut(s)	104, 159, 249, 369, 679, 1170, 1244, 1759, 2086
MlyI	GAGTC	6 cut(s)	178, 1321, 1379, 2006, 2006, 2064
MroI	TCCGGA	1 cut(s)	1715
MroXI	GAANNNNTTC	1 cut(s)	134
MseI	TTAA	5 cut(s)	594, 840, 1506, 2022, 2084
MslI	CAYNNNNRTG	4 cut(s)	306, 1031, 1380, 2114
MspA1I	CMGCKG	1 cut(s)	776
MspI	CCGG	5 cut(s)	1716, 1727, 1748, 2009, 2080
MspR9I	CCNGG	6 cut(s)	357, 726, 881, 1749, 2009, 2080
MunI	CAATTG	1 cut(s)	104
Mva1269I	GAATGC	3 cut(s)	959, 2065, 2255
MvaI	CCWGG	3 cut(s)	357, 726, 881
MvnI	CGCG	2 cut(s)	150, 2313
MwoI	GCNNNNNNNGC	9 cut(s)	66, 464, 782, 866, 1442, 1574, 1865, 1874, 2305
NciI	CCSGG	3 cut(s)	1749, 2009, 2080
NcoI	CCATGG	2 cut(s)	1050, 1165
NdeI	CATATG	1 cut(s)	646
NdeII	GATC	6 cut(s)	191, 364, 983, 1819, 2029, 2219
NlaIV	GGNNCC	7 cut(s)	226, 321, 354, 603, 1658, 1821, 1837
NmuCI	GTSAC	1 cut(s)	1900
NspI	RCATGY	1 cut(s)	1738
OliI	CACNNNNGTG	1 cut(s)	1031
PagI	TCATGA	1 cut(s)	1468
PaqCI	CACCTGC	1 cut(s)	244
PauI	GCGCGC	1 cut(s)	2311
PciI	ACATGT	1 cut(s)	1734
PcsI	WCGNNNNNNNCGW	1 cut(s)	756
PctI	GAATGC	3 cut(s)	959, 2065, 2255
PdmI	GAANNNNTTC	1 cut(s)	134
PfeI	GAWTC	2 cut(s)	13, 1475
PflMI	CCANNNNNTGG	2 cut(s)	201, 287
PfoI	TCCNGGA	2 cut(s)	1747, 2007
PinAI	ACCGGT	1 cut(s)	1726
PkrI	GCNGC	7 cut(s)	62, 818, 1447, 1701, 1809, 1870, 2310
PleI	GAGTC	6 cut(s)	177, 1321, 1378, 2005, 2006, 2064
PpsI	GAGTC	6 cut(s)	177, 1321, 1378, 2005, 2006, 2064
PpuMI	RGGWCCY	1 cut(s)	352
PscI	ACATGT	1 cut(s)	1734
Psp5II	RGGWCCY	1 cut(s)	352
Psp6I	CCWGG	3 cut(s)	355, 724, 879
PspEI	GGTNACC	1 cut(s)	1900
PspGI	CCWGG	3 cut(s)	355, 724, 879
PspN4I	GGNNCC	7 cut(s)	226, 321, 354, 603, 1658, 1821, 1837
PspPI	GGNCC	7 cut(s)	260, 352, 770, 953, 1403, 2132, 2225
PspPPI	RGGWCCY	1 cut(s)	352
PstI	CTGCAG	1 cut(s)	808
PsuI	RGATCY	2 cut(s)	983, 1819
PteI	GCGCGC	1 cut(s)	2311
PvuII	CAGCTG	1 cut(s)	776
RsaI	GTAC	1 cut(s)	876
RsaNI	GTAC	1 cut(s)	875
RseI	CAYNNNNRTG	4 cut(s)	306, 1031, 1380, 2114
SaqAI	TTAA	5 cut(s)	594, 840, 1506, 2022, 2084
SatI	GCNGC	7 cut(s)	61, 817, 1446, 1700, 1808, 1869, 2309
Sau3AI	GATC	6 cut(s)	191, 364, 983, 1819, 2029, 2219
Sau96I	GGNCC	7 cut(s)	260, 352, 770, 953, 1403, 2132, 2225
SchI	GAGTC	6 cut(s)	178, 1321, 1379, 2006, 2006, 2064
ScrFI	CCNGG	6 cut(s)	357, 726, 881, 1749, 2009, 2080
SduI	GDGCHC	3 cut(s)	1659, 1777, 2289
SfaNI	GCATC	3 cut(s)	401, 1254, 2108
SfcI	CTRYAG	2 cut(s)	804, 2260
SinI	GGWCC	6 cut(s)	260, 352, 770, 953, 1403, 2132
SmiMI	CAYNNNNRTG	4 cut(s)	306, 1031, 1380, 2114
SmlI	CTYRAG	1 cut(s)	1097
SmoI	CTYRAG	1 cut(s)	1097
SpeI	ACTAGT	1 cut(s)	1058
Sse9I	AATT	9 cut(s)	104, 159, 249, 369, 679, 1170, 1244, 1759, 2086
SsiI	CCGC	5 cut(s)	816, 1324, 1906, 2067, 2135
SspI	AATATT	1 cut(s)	838
SspMI	CTAG	8 cut(s)	264, 491, 608, 942, 1059, 1088, 1512, 1569
StyD4I	CCNGG	6 cut(s)	355, 724, 879, 1747, 2007, 2078
StyI	CCWWGG	6 cut(s)	892, 1050, 1165, 1438, 1545, 1827
TaaI	ACNGT	8 cut(s)	120, 583, 1241, 1286, 1982, 2160, 2239, 2291
TaiI	ACGT	2 cut(s)	244, 1159
TaqI	TCGA	1 cut(s)	144
TaqII	GACCGA	1 cut(s)	941
TasI	AATT	9 cut(s)	104, 159, 249, 369, 679, 1170, 1244, 1759, 2086
TatI	WGTACW	1 cut(s)	874
TauI	GCSGC	1 cut(s)	819
TfiI	GAWTC	2 cut(s)	13, 1475
Tru1I	TTAA	5 cut(s)	594, 840, 1506, 2022, 2084
Tru9I	TTAA	5 cut(s)	594, 840, 1506, 2022, 2084
TscAI	CASTG	5 cut(s)	588, 637, 808, 1038, 2296
TseFI	GTSAC	1 cut(s)	1900
TseI	GCWGC	6 cut(s)	60, 1445, 1699, 1807, 1868, 2308
Tsp45I	GTSAC	1 cut(s)	1900
TspGWI	ACGGA	1 cut(s)	1438
TspRI	CASTG	5 cut(s)	588, 637, 808, 1038, 2296
Van91I	CCANNNNNTGG	2 cut(s)	201, 287
VpaK11BI	GGWCC	6 cut(s)	260, 352, 770, 953, 1403, 2132
XapI	RAATTY	4 cut(s)	369, 679, 1244, 1759
XceI	RCATGY	1 cut(s)	1738
XcmI	CCANNNNNNNNNTGG	1 cut(s)	202
XmiI	GTMKAC	1 cut(s)	711
XmnI	GAANNNNTTC	1 cut(s)	134
XspI	CTAG	8 cut(s)	264, 491, 608, 942, 1059, 1088, 1512, 1569

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.