Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0007837

Physical Location & Seq

Genomic Coordinates Reverse (-)

26631631 .. 26636373

Gene Structure 4743 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1173600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2334 bp

ATGCGAACTCCGATTCTGCTTCTGCTGTTTGTTCTCCTCTTGATTTTCTGCTGCTTAGGGGGTTTTTCTCTAAATATTTACTTGTTTCCTTCTCAATTGCCAGACCAACCGTTGTCTATGAAAAATTTCTCAGTCGAACGCGCAAGGTCAACGTTGATGGTTCTTCCATTTGCTTCATCAAAGCGATCCAAAGCATGGGCGGTCAAGCCTTTTACGGGAATCCTTGCACCTGCACCTTCTCCAATTCATCTAGGTCCTAGCAGTCCTCACCCACCACAGCACGGCCATTATCGTCATCATCATGTGAGAGTGAAACCCCGTGCTGTTGCTCCAGCTCCATCAAATGACCCAGGTTGTGACCAAATTTGTGTAGAGCCACTTACCACATCTCCATTTGGTTCACCTTGTGGTTGTGTGTTTCCCATGAAAGTCAAACTTTTACTGGACATAGCTCCTTATGCTATTTTTCCAGTAATGAGTGAGCTAGAGATTGAGATTGCTGAAGGCACGTATCTGACACAAAGTCAAGTGAAAATAATGGGTGCAAGTGCTGATAGTCAAAATCAAGGAAGAACAGTGGTGGATATTAACTTGGTTCCACTAGGGGAGAAGTTTGATAATACCACTGCGATACTGACATATGATAGATTTCAGCATAAGAAAGTGCCTCTGAATTTGACACTTTTTGGAAACTATGAAGTGGTATACATTAGTTATCCAGGGCTACCTCCTTCACCGCCATATGAGATTTACAATGGGCATGATCCAGCTGGAATTGCTGGAGGTCTCCCTTTCACCGCGGATTTTGGCGGCAAGAACCAGAGGATGAATATTAGAACGATTGTGATTATTGCTCTGTCTGCATTTGTAGTCCTGGTGGTTCTCCTTGCGGCTATCTGTATCTTTGTGAAGGGGAGGAGAGTTGGAAGACCATCTAGCGCAGTCAGTCCTGTATTCACACCTTCTGTTCACAAAAGATCTGGTATCGGGTCTATCTTATCAAGTAGCATTGCAAGCTCCACTTCAGTGTCCCTAATGTCCACCATGGCTACCAGTATTCTCTCTGTCAAAACATTTCCACTAGCTGAGCTTGAGAAAGCAACAAATAAGTTCAGTTCCCAAAGGGTTTTGGGAGAAGGGGGATTTGGACGTGTTTACCATGGAATTATGGAAGATGGGACTGAAGTTGCAGTCAAAGTGCTTACAAGGGATAATCAGAATCAGAATGGAGACCGTGAATTTATTGCAGAAGTTGAGATGCTAAGCCGTTTGCATCACCGTAATCTTGTGAAGTTGATAGGCATTTGTATTGAAGGGCAGACACGCAGTTTGGTATGTGAGATTGTTCGCAATGGAAGTGTTGAATCTCACTTGCACGGCATTGACAAGATAAATGGGCCTCTTGACTGGGATGCAAGGATGAAGATTGCCCTTGGGGCAGCAAGAGGATTAGCCTATCTTCACGAAGATTCTAATCCTCGTGTTATTCACCGAGATTTTAAGGCTAGTAACGTTTTGCTGGAAGCTGACTTCACACCCAAGGTTTCAGATTTTGGGTTAGCTAGGGAAGCAACTGAGGGAAGTCATCACATCTCCACTAGGGTCATGGGAACTTTTGGGTATGTTGCCCCAGAATATGCAATGACAGGGCACCTACTTGTCAAGAGCGATGTTTATAGTTATGGCGTTGTGCTGCTGGAACTCCTCTCTGGAAGAAAACCGGTTGACATGTCCCACTCTCCGGGACAGGAGAATTTAGTAACATGGGCACGACCATTGCTAACGAGCAGAGAAGGCTTGCAGCAGTTGGTGGATCCCACCTTAGCTGGAACCCATGACTTTGACGACATGGCTAAAGTGGCAGCCATTGCTTCCATGTGCGTTCACCCTGAGGTGACCCACAGACCTTTTATGGGTGAAGTTGTGCAGGCTCTAAAGCTGATATACAATGATAACGATGAGACTGGTGGGGACTGTTATAGTCAAAAGGAGTCTTCCGTCCAGGACTCTGACTTTAAAGGTGATCTTGCCCCATCTGATAGCAGTTGGTGGAATGCTGGTGGACTCACTCCCCGGATGAATTATGGGCATGCCTCTTCATTCATCACAATGGAGTATAGTTCTGGACCACTTGAAGATATGGAAAACAGACCGTTTTCAACTTCAAGTTTGGTAGGGGATGAGATATTGTTACCAATCAGACACGGCAATAGGTCAGGGCCGTTGAGAACAGTCCGAAGCAAGCCAGCATTCTACAGAGTAAGAGGAAGTAGGAGCGACCACGGGGGACTGCTCTCGAAGAAGCGTGCTTGGAACGATGGCTTCTGGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010065 GO:0010068 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0042335 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

778

Amino Acids

85.01

Weight (kDa)

7.96

Isoelectric Point (pI)

43.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALE2_N	PF23180	125 - 243	1.2e-55	Receptor-like PK ALE2 N-terminal domain
Pkinase	PF00069	372 - 584	1.5e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	374 - 644	3.5e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007837)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20300
fragaria_vesca	FvH4_2g20190 FvH4_2g20190 FvH4_2g20190 FvH4_2g20190 FvH4_2g20190 FvH4_2g20190
malus_domestica	MD05G1185400.v1.1 MD10G1173600.v1.1
prunus_persica	Prupe.8G212200_v2.0.a1 Prupe.8G212200_v2.0.a1
pyrus_communis	pycom05g17020 pycom10g14970
rosa_chinensis	RchiOBHm_Chr6g0285961
rosa_laevigata	RLG00000012594
rosa_multiflora	Rmu_sc0004808.1_g000010 Rmu_sc0005513.1_g000001
rosa_roxburghii	Rroxscaffold_7G00182570
rosa_rugosa	Rorug06G0177700
rosa_samantha	Rh6AG289800 Rh6BG291700 Rh6CG292700 Rh6DG284800
rosa_wichuraiana	Rw6G024970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	238
Acc36I	ACCTGC	1 cut(s)	238
AccB1I	GGYRCC	1 cut(s)	1650
AccB7I	CCANNNNNTGG	1 cut(s)	195
AccI	GTMKAC	1 cut(s)	705
AccII	CGCG	2 cut(s)	141, 800
AciI	CCGC	6 cut(s)	200, 737, 798, 800, 810, 890
AclI	AACGTT	2 cut(s)	152, 1512
AclWI	GGATC	4 cut(s)	180, 758, 1808, 1821
AcoI	YGGCCR	1 cut(s)	283
AcsI	RAATTY	5 cut(s)	124, 363, 673, 1238, 1753
AcuI	CTGAAG	3 cut(s)	522, 1008, 1203
AdeI	CACNNNGTG	1 cut(s)	407
AfiI	CCNNNNNNNGG	5 cut(s)	195, 215, 281, 1741, 1913
AflIII	ACRYGT	2 cut(s)	1150, 1728
AgeI	ACCGGT	1 cut(s)	1720
AgsI	TTSAA	5 cut(s)	1313, 1364, 2135, 2160, 2166
AhdI	GACNNNNNGTC	1 cut(s)	522
AjiI	CACGTC	1 cut(s)	1151
AjnI	CCWGG	4 cut(s)	349, 718, 873, 2001
AjuI	GAANNNNNNNTTGG	2 cut(s)	1128, 1160
AleI	CACNNNNGTG	1 cut(s)	1025
Alw26I	GTCTC	3 cut(s)	791, 1224, 1955
AlwI	GGATC	4 cut(s)	180, 758, 1808, 1821
AoxI	GGCC	3 cut(s)	283, 1397, 2219
ApeKI	GCWGC	5 cut(s)	51, 1439, 1693, 1801, 1862
ApoI	RAATTY	5 cut(s)	124, 363, 673, 1238, 1753
ArsI	GACNNNNNNTTYG	2 cut(s)	1312, 1344
AsiGI	ACCGGT	1 cut(s)	1720
Asp700I	GAANNNNTTC	1 cut(s)	125
AspLEI	GCGC	2 cut(s)	143, 941
AspS9I	GGNCC	4 cut(s)	254, 1397, 2126, 2219
AsuC2I	CCSGG	2 cut(s)	1743, 2074
AvaII	GGWCC	2 cut(s)	254, 2126
AxyI	CCTNAGG	1 cut(s)	1890
BaeGI	GKGCMC	2 cut(s)	1653, 1771
BamHI	GGATCC	1 cut(s)	1813
BanI	GGYRCC	1 cut(s)	1650
BauI	CACGAG	1 cut(s)	1479
BbsI	GAAGAC	2 cut(s)	934, 1986
BbvI	GCAGC	5 cut(s)	38, 1451, 1680, 1813, 1874
BccI	CCATC	6 cut(s)	151, 346, 940, 1169, 2041, 2312
BceAI	ACGGC	5 cut(s)	298, 1251, 1393, 2206, 2221
BciT130I	CCWGG	4 cut(s)	351, 720, 875, 2003
BcnI	CCSGG	2 cut(s)	1743, 2074
BcoDI	GTCTC	3 cut(s)	791, 1224, 1955
BfaI	CTAG	9 cut(s)	251, 258, 485, 602, 936, 1082, 1506, 1563, 1599
BfmI	CTRYAG	1 cut(s)	2254
BfuAI	ACCTGC	1 cut(s)	238
BglI	GCCNNNNNGGC	1 cut(s)	1436
BglII	AGATCT	1 cut(s)	977
BisI	GCNGC	7 cut(s)	52, 811, 891, 1440, 1694, 1802, 1863
BlpI	GCTNAGC	2 cut(s)	1086, 1262
BlsI	GCNGC	7 cut(s)	53, 812, 892, 1441, 1695, 1803, 1864
Bme1390I	CCNGG	6 cut(s)	351, 720, 875, 1743, 2003, 2074
Bme18I	GGWCC	2 cut(s)	254, 2126
BmeRI	GACNNNNNGTC	1 cut(s)	522
BmgBI	CACGTC	1 cut(s)	1151
BmgT120I	GGNCC	4 cut(s)	254, 1397, 2126, 2219
BmiI	GGNNCC	4 cut(s)	597, 1652, 1815, 1831
BmrFI	CCNGG	6 cut(s)	351, 720, 875, 1743, 2003, 2074
BmrI	ACTGGG	1 cut(s)	1417
BmsI	GCATC	3 cut(s)	1248, 1282, 1402
BmuI	ACTGGG	1 cut(s)	1417
BpiI	GAAGAC	2 cut(s)	934, 1986
BpmI	CTGGAG	2 cut(s)	315, 801
Bpu10I	CCTNAGC	2 cut(s)	55, 1822
Bpu1102I	GCTNAGC	2 cut(s)	1086, 1262
BpuEI	CTTGAG	1 cut(s)	1112
BpuMI	CCSGG	2 cut(s)	1743, 2074
BsaAI	YACGTR	1 cut(s)	510
BsaBI	GATNNNNATC	1 cut(s)	1473
BsaI	GGTCTC	2 cut(s)	791, 1224
BsaJI	CCNNGG	9 cut(s)	349, 719, 798, 1044, 1159, 1432, 1539, 2072, 2281
BsaWI	WCCGGW	1 cut(s)	1720
BsaXI	ACNNNNNCTCC	2 cut(s)	373, 403
Bsc4I	CCNNNNNNNGG	5 cut(s)	195, 215, 281, 1741, 1913
Bse118I	RCCGGY	1 cut(s)	1720
Bse1I	ACTGG	5 cut(s)	447, 470, 1053, 1412, 1969
Bse21I	CCTNAGG	1 cut(s)	1890
Bse3DI	GCAATG	5 cut(s)	1008, 1357, 1647, 1775, 1866
Bse8I	GATNNNNATC	1 cut(s)	1473
BseBI	CCWGG	4 cut(s)	351, 720, 875, 2003
BseDI	CCNNGG	9 cut(s)	349, 719, 798, 1044, 1159, 1432, 1539, 2072, 2281
BseGI	GGATG	5 cut(s)	831, 1417, 1425, 2082, 2185
BseJI	GATNNNNATC	1 cut(s)	1473
BseLI	CCNNNNNNNGG	5 cut(s)	195, 215, 281, 1741, 1913
BseMI	GCAATG	5 cut(s)	1008, 1357, 1647, 1775, 1866
BseMII	CTCAG	4 cut(s)	144, 1077, 1566, 1881
BseNI	ACTGG	5 cut(s)	447, 470, 1053, 1412, 1969
BseRI	GAGGAG	3 cut(s)	26, 931, 1694
BseSI	GKGCMC	2 cut(s)	1653, 1771
BseXI	GCAGC	5 cut(s)	38, 1451, 1680, 1813, 1874
BsgI	GTGCAG	2 cut(s)	216, 1946
Bsh1236I	CGCG	2 cut(s)	141, 800
BshFI	GGCC	3 cut(s)	285, 1399, 2221
BshNI	GGYRCC	1 cut(s)	1650
BshTI	ACCGGT	1 cut(s)	1720
BsiSI	CCGG	3 cut(s)	1721, 1742, 2074
BslFI	GGGAC	6 cut(s)	1015, 1192, 1717, 1758, 1985, 2301
BslI	CCNNNNNNNGG	5 cut(s)	195, 215, 281, 1741, 1913
BsmAI	GTCTC	3 cut(s)	791, 1224, 1955
BsmFI	GGGAC	6 cut(s)	1015, 1192, 1717, 1758, 1985, 2301
BsmI	GAATGC	2 cut(s)	2059, 2249
BsnI	GGCC	3 cut(s)	285, 1399, 2221
Bso31I	GGTCTC	2 cut(s)	791, 1224
Bsp1286I	GDGCHC	2 cut(s)	1653, 1771
Bsp143I	GATC	5 cut(s)	185, 763, 977, 1813, 2023
Bsp1720I	GCTNAGC	2 cut(s)	1086, 1262
Bsp19I	CCATGG	2 cut(s)	1044, 1159
BspACI	CCGC	6 cut(s)	200, 737, 798, 800, 810, 890
BspANI	GGCC	3 cut(s)	285, 1399, 2221
BspCNI	CTCAG	4 cut(s)	143, 1078, 1567, 1882
BspFNI	CGCG	2 cut(s)	141, 800
BspLI	GGNNCC	4 cut(s)	597, 1652, 1815, 1831
BspMI	ACCTGC	1 cut(s)	238
BspPI	GGATC	4 cut(s)	180, 758, 1808, 1821
BspT107I	GGYRCC	1 cut(s)	1650
BspTNI	GGTCTC	2 cut(s)	791, 1224
BsrDI	GCAATG	5 cut(s)	1008, 1357, 1647, 1775, 1866
BsrFI	RCCGGY	1 cut(s)	1720
BsrI	ACTGG	5 cut(s)	447, 470, 1053, 1412, 1969
BssAI	RCCGGY	1 cut(s)	1720
BssECI	CCNNGG	9 cut(s)	349, 719, 798, 1044, 1159, 1432, 1539, 2072, 2281
BssMI	GATC	5 cut(s)	185, 763, 977, 1813, 2023
BssNAI	GTATAC	1 cut(s)	706
BssSI	CACGAG	1 cut(s)	1479
BssT1I	CCWWGG	4 cut(s)	1044, 1159, 1432, 1539
Bst1107I	GTATAC	1 cut(s)	706
Bst2BI	CACGAG	1 cut(s)	1479
Bst2UI	CCWGG	4 cut(s)	351, 720, 875, 2003
Bst4CI	ACNGT	7 cut(s)	111, 577, 1235, 1280, 1976, 2154, 2233
Bst6I	CTCTTC	1 cut(s)	2101
BstAPI	GCANNNNNTGC	1 cut(s)	1868
BstBAI	YACGTR	1 cut(s)	510
BstC8I	GCNNGC	7 cut(s)	1015, 1799, 1929, 2091, 2243, 2247, 2307
BstDEI	CTNAG	7 cut(s)	55, 130, 1086, 1262, 1575, 1822, 1890
BstDSI	CCRYGG	4 cut(s)	798, 1044, 1159, 2281
BstEII	GGTNACC	1 cut(s)	1894
BstF5I	GGATG	5 cut(s)	831, 1417, 1425, 2082, 2185
BstFNI	CGCG	2 cut(s)	141, 800
BstHHI	GCGC	2 cut(s)	143, 941
BstKTI	GATC	5 cut(s)	188, 766, 980, 1816, 2026
BstMAI	GTCTC	3 cut(s)	791, 1224, 1955
BstMBI	GATC	5 cut(s)	185, 763, 977, 1813, 2023
BstMWI	GCNNNNNNNGC	9 cut(s)	458, 776, 860, 1014, 1436, 1568, 1794, 1859, 1868
BstNI	CCWGG	4 cut(s)	351, 720, 875, 2003
BstNSI	RCATGY	2 cut(s)	1732, 2093
BstPI	GGTNACC	1 cut(s)	1894
BstSCI	CCNGG	6 cut(s)	349, 718, 873, 1741, 2001, 2072
BstSFI	CTRYAG	1 cut(s)	2254
BstSLI	GKGCMC	2 cut(s)	1653, 1771
BstUI	CGCG	2 cut(s)	141, 800
BstV1I	GCAGC	5 cut(s)	38, 1451, 1680, 1813, 1874
BstV2I	GAAGAC	2 cut(s)	934, 1986
BstX2I	RGATCY	2 cut(s)	977, 1813
BstYI	RGATCY	2 cut(s)	977, 1813
BstZ17I	GTATAC	1 cut(s)	706
Bsu36I	CCTNAGG	1 cut(s)	1890
BsuRI	GGCC	3 cut(s)	285, 1399, 2221
BtgI	CCRYGG	4 cut(s)	798, 1044, 1159, 2281
BtgZI	GCGATG	1 cut(s)	1683
BtrI	CACGTC	1 cut(s)	1151
BtsCI	GGATG	5 cut(s)	831, 1417, 1425, 2082, 2185
BtsI	GCAGTG	1 cut(s)	624
BtsIMutI	CAGTG	3 cut(s)	582, 624, 1032
BveI	ACCTGC	1 cut(s)	238
Cac8I	GCNNGC	7 cut(s)	1015, 1799, 1929, 2091, 2243, 2247, 2307
CfoI	GCGC	2 cut(s)	143, 941
Cfr10I	RCCGGY	1 cut(s)	1720
Cfr13I	GGNCC	4 cut(s)	254, 1397, 2126, 2219
Cfr42I	CCGCGG	1 cut(s)	801
CspAI	ACCGGT	1 cut(s)	1720
DdeI	CTNAG	7 cut(s)	55, 130, 1086, 1262, 1575, 1822, 1890
DpnI	GATC	5 cut(s)	187, 765, 979, 1815, 2025
DpnII	GATC	5 cut(s)	185, 763, 977, 1813, 2023
DraI	TTTAAA	1 cut(s)	2017
DraIII	CACNNNGTG	1 cut(s)	407
DriI	GACNNNNNGTC	1 cut(s)	522
EaeI	YGGCCR	1 cut(s)	283
Eam1104I	CTCTTC	1 cut(s)	2101
Eam1105I	GACNNNNNGTC	1 cut(s)	522
EarI	CTCTTC	1 cut(s)	2101
Eco130I	CCWWGG	4 cut(s)	1044, 1159, 1432, 1539
Eco31I	GGTCTC	2 cut(s)	791, 1224
Eco47I	GGWCC	2 cut(s)	254, 2126
Eco57I	CTGAAG	3 cut(s)	522, 1008, 1203
Eco81I	CCTNAGG	1 cut(s)	1890
Eco91I	GGTNACC	1 cut(s)	1894
EcoO109I	RGGNCCY	1 cut(s)	254
EcoO65I	GGTNACC	1 cut(s)	1894
EcoRII	CCWGG	4 cut(s)	349, 718, 873, 2001
EcoT14I	CCWWGG	4 cut(s)	1044, 1159, 1432, 1539
ErhI	CCWWGG	4 cut(s)	1044, 1159, 1432, 1539
FalI	AAGNNNNNCTT	6 cut(s)	420, 452, 1006, 1038, 2010, 2042
FaqI	GGGAC	6 cut(s)	1015, 1192, 1717, 1758, 1985, 2301
FauNDI	CATATG	2 cut(s)	640, 742
FblI	GTMKAC	1 cut(s)	705
Fnu4HI	GCNGC	7 cut(s)	52, 811, 891, 1440, 1694, 1802, 1863
FokI	GGATG	5 cut(s)	838, 1424, 1432, 2089, 2192
Fsp4HI	GCNGC	7 cut(s)	52, 811, 891, 1440, 1694, 1802, 1863
FspBI	CTAG	9 cut(s)	251, 258, 485, 602, 936, 1082, 1506, 1563, 1599
GlaI	GCGC	2 cut(s)	142, 940
GluI	GCNGC	7 cut(s)	52, 811, 891, 1440, 1694, 1802, 1863
GsuI	CTGGAG	2 cut(s)	315, 801
HaeIII	GGCC	3 cut(s)	285, 1399, 2221
HapII	CCGG	3 cut(s)	1721, 1742, 2074
HhaI	GCGC	2 cut(s)	143, 941
Hin6I	GCGC	2 cut(s)	141, 939
HinP1I	GCGC	2 cut(s)	141, 939
HincII	GTYRAC	2 cut(s)	150, 1726
HindII	GTYRAC	2 cut(s)	150, 1726
HinfI	GANTC	8 cut(s)	13, 219, 1219, 1364, 1469, 1991, 2006, 2064
HpaII	CCGG	3 cut(s)	1721, 1742, 2074
Hpy166II	GTNNAC	9 cut(s)	150, 401, 706, 970, 1041, 1156, 1726, 1885, 2063
Hpy188III	TCNNGA	7 cut(s)	40, 1403, 1463, 1663, 1710, 2124, 2296
Hpy8I	GTNNAC	9 cut(s)	150, 401, 706, 970, 1041, 1156, 1726, 1885, 2063
HpyAV	CCTTC	9 cut(s)	99, 246, 497, 741, 904, 972, 1130, 1307, 1787
HpyCH4III	ACNGT	7 cut(s)	111, 577, 1235, 1280, 1976, 2154, 2233
HpyCH4IV	ACGT	4 cut(s)	152, 509, 1150, 1512
HpyF10VI	GCNNNNNNNGC	9 cut(s)	458, 776, 860, 1014, 1436, 1568, 1794, 1859, 1868
HpyF3I	CTNAG	7 cut(s)	55, 130, 1086, 1262, 1575, 1822, 1890
HpySE526I	ACGT	4 cut(s)	152, 509, 1150, 1512
HspAI	GCGC	2 cut(s)	141, 939
KspI	CCGCGG	1 cut(s)	801
Kzo9I	GATC	5 cut(s)	185, 763, 977, 1813, 2023
LmnI	GCTCC	5 cut(s)	334, 340, 457, 1022, 2274
Lsp1109I	GCAGC	5 cut(s)	38, 1451, 1680, 1813, 1874
LweI	GCATC	3 cut(s)	1248, 1282, 1402
MaeI	CTAG	9 cut(s)	251, 258, 485, 602, 936, 1082, 1506, 1563, 1599
MaeII	ACGT	4 cut(s)	152, 509, 1150, 1512
MaeIII	GTNAC	5 cut(s)	356, 1508, 1759, 1894, 2190
MalI	GATC	5 cut(s)	187, 765, 979, 1815, 2025
MboI	GATC	5 cut(s)	185, 763, 977, 1813, 2023
MfeI	CAATTG	1 cut(s)	95
MflI	RGATCY	2 cut(s)	977, 1813
MhlI	GDGCHC	2 cut(s)	1653, 1771
MlyI	GAGTC	3 cut(s)	2000, 2000, 2058
MmeI	TCCRAC	1 cut(s)	904
MroXI	GAANNNNTTC	1 cut(s)	125
MseI	TTAA	3 cut(s)	588, 1500, 2016
MslI	CAYNNNNRTG	3 cut(s)	300, 1025, 2108
MspA1I	CMGCKG	2 cut(s)	770, 800
MspI	CCGG	3 cut(s)	1721, 1742, 2074
MspR9I	CCNGG	6 cut(s)	351, 720, 875, 1743, 2003, 2074
MunI	CAATTG	1 cut(s)	95
Mva1269I	GAATGC	2 cut(s)	2059, 2249
MvaI	CCWGG	4 cut(s)	351, 720, 875, 2003
MvnI	CGCG	2 cut(s)	141, 800
MwoI	GCNNNNNNNGC	9 cut(s)	458, 776, 860, 1014, 1436, 1568, 1794, 1859, 1868
NciI	CCSGG	2 cut(s)	1743, 2074
NcoI	CCATGG	2 cut(s)	1044, 1159
NdeI	CATATG	2 cut(s)	640, 742
NdeII	GATC	5 cut(s)	185, 763, 977, 1813, 2023
NlaIV	GGNNCC	4 cut(s)	597, 1652, 1815, 1831
NmuCI	GTSAC	2 cut(s)	356, 1894
NspI	RCATGY	2 cut(s)	1732, 2093
OliI	CACNNNNGTG	1 cut(s)	1025
PaeI	GCATGC	1 cut(s)	2093
PaqCI	CACCTGC	1 cut(s)	238
PciI	ACATGT	1 cut(s)	1728
PctI	GAATGC	2 cut(s)	2059, 2249
PdmI	GAANNNNTTC	1 cut(s)	125
PfeI	GAWTC	5 cut(s)	13, 219, 1219, 1364, 1469
PflMI	CCANNNNNTGG	1 cut(s)	195
PfoI	TCCNGGA	2 cut(s)	1741, 2001
PinAI	ACCGGT	1 cut(s)	1720
PkrI	GCNGC	7 cut(s)	53, 812, 892, 1441, 1695, 1803, 1864
PleI	GAGTC	3 cut(s)	1999, 2000, 2058
PpsI	GAGTC	3 cut(s)	1999, 2000, 2058
Ppu21I	YACGTR	1 cut(s)	510
PpuMI	RGGWCCY	1 cut(s)	254
PscI	ACATGT	1 cut(s)	1728
Psp1406I	AACGTT	2 cut(s)	152, 1512
Psp5II	RGGWCCY	1 cut(s)	254
Psp6I	CCWGG	4 cut(s)	349, 718, 873, 2001
PspEI	GGTNACC	1 cut(s)	1894
PspGI	CCWGG	4 cut(s)	349, 718, 873, 2001
PspN4I	GGNNCC	4 cut(s)	597, 1652, 1815, 1831
PspPI	GGNCC	4 cut(s)	254, 1397, 2126, 2219
PspPPI	RGGWCCY	1 cut(s)	254
PsuI	RGATCY	2 cut(s)	977, 1813
PvuII	CAGCTG	1 cut(s)	770
RseI	CAYNNNNRTG	3 cut(s)	300, 1025, 2108
SacII	CCGCGG	1 cut(s)	801
SaqAI	TTAA	3 cut(s)	588, 1500, 2016
SatI	GCNGC	7 cut(s)	52, 811, 891, 1440, 1694, 1802, 1863
Sau3AI	GATC	5 cut(s)	185, 763, 977, 1813, 2023
Sau96I	GGNCC	4 cut(s)	254, 1397, 2126, 2219
SchI	GAGTC	3 cut(s)	2000, 2000, 2058
ScrFI	CCNGG	6 cut(s)	351, 720, 875, 1743, 2003, 2074
SduI	GDGCHC	2 cut(s)	1653, 1771
SfaNI	GCATC	3 cut(s)	1248, 1282, 1402
SfcI	CTRYAG	1 cut(s)	2254
Sfr303I	CCGCGG	1 cut(s)	801
SgrBI	CCGCGG	1 cut(s)	801
SinI	GGWCC	2 cut(s)	254, 2126
SmiMI	CAYNNNNRTG	3 cut(s)	300, 1025, 2108
SmlI	CTYRAG	1 cut(s)	1091
SmoI	CTYRAG	1 cut(s)	1091
SphI	GCATGC	1 cut(s)	2093
SsiI	CCGC	6 cut(s)	200, 737, 798, 800, 810, 890
SspI	AATATT	2 cut(s)	76, 832
SspMI	CTAG	9 cut(s)	251, 258, 485, 602, 936, 1082, 1506, 1563, 1599
StyD4I	CCNGG	6 cut(s)	349, 718, 873, 1741, 2001, 2072
StyI	CCWWGG	4 cut(s)	1044, 1159, 1432, 1539
TaaI	ACNGT	7 cut(s)	111, 577, 1235, 1280, 1976, 2154, 2233
TaiI	ACGT	4 cut(s)	155, 512, 1153, 1515
TaqI	TCGA	2 cut(s)	135, 2297
TauI	GCSGC	2 cut(s)	813, 893
TfiI	GAWTC	5 cut(s)	13, 219, 1219, 1364, 1469
Tru1I	TTAA	3 cut(s)	588, 1500, 2016
Tru9I	TTAA	3 cut(s)	588, 1500, 2016
TscAI	CASTG	3 cut(s)	582, 631, 1032
TseFI	GTSAC	2 cut(s)	356, 1894
TseI	GCWGC	5 cut(s)	51, 1439, 1693, 1801, 1862
Tsp45I	GTSAC	2 cut(s)	356, 1894
TspGWI	ACGGA	1 cut(s)	1987
TspRI	CASTG	3 cut(s)	582, 631, 1032
Van91I	CCANNNNNTGG	1 cut(s)	195
VpaK11BI	GGWCC	2 cut(s)	254, 2126
XapI	RAATTY	5 cut(s)	124, 363, 673, 1238, 1753
XceI	RCATGY	2 cut(s)	1732, 2093
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1603
XmiI	GTMKAC	1 cut(s)	705
XmnI	GAANNNNTTC	1 cut(s)	125
XspI	CTAG	9 cut(s)	251, 258, 485, 602, 936, 1082, 1506, 1563, 1599

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.