AT2G27020

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0014407
Physical Location & Seq
Reverse (-)
11528230 .. 11531178
2949 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27020.1

Sequence Viewer

Length: 750 bp
ATGAGTAGCATTGGAACTGGGTACGATCTCTCCGTCACCACTTTCTCTCCCGATGGCCGTGTTTTCCAGATCGAGTACGCCGCTAAAGCTGTCGACAACAGTGGGACTGTTGTTGGAATCAAGTGCAAAGATGGGATTGTTATGGGAGTTGAGAAACTTATTGCATCAAAGATGATGCTACCAGGTTCCAACAGGAGAATCCATTCTGTTCATCGTCATGCTGGCATGGCTGTTGCTGGGCTTGCAGCTGATGGGAGGCAAATTGTTGCGCGTGCTAAGTCTGAAGCACGAAGCTATGAGAGTGTTTATGGCGATGCTGTACCTGTGAAGGAACTTTCTGAGCGTGTTGCAAGTTATGTACATCTGTGTACCCTGTATTGGTGGCTCAGGCCTTTTGGCTGTGGGGTTATTCTTGGAGGTTATGATAGAGATGGACCTCAATTGTACATGATTGAACCATCAGGCATATCATATAGATACTTTGGTGCGGCCATTGGCAAAGGAAAGCAAGCTGCCAAAACGGAAATTGAGAAATTAAATTTATCTGAGATGACATGCAAAGAAGGCGTTATCGAGGTGGCGAAAATCATCTACAAGCTACACGATGAAGCCAAGGACAAGGCTTTTGAATTGGAGATGAGCTGGATATGCGAGGAGTCAAAACGAGAGCATCAGAAGGTCCCTGATGATCTTCTGGAAGAAGCAAAGACGGCAGCTAAAACTGCGCTCGAGGAGATGGATGCTGACTAA

Protein Analysis

249

Amino Acids

27.38

Weight (kDa)

5.93

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome_A_N PF10584 8 - 30 2.2e-13 Proteasome subunit A N-terminal signature
Proteasome PF00227 31 - 216 9.7e-53 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014407)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27020 AT2G27020
fragaria_vesca FvH4_5g23670
malus_domestica MD14G1092100.v1.1
prunus_persica Prupe.7G022800_v2.0.a1 Prupe.7G022800_v2.0.a1
pyrus_communis pycom14g08930 pycom14g08970
rosa_chinensis RchiOBHm_Chr7g0212971
rosa_laevigata RLG00000002841
rosa_multiflora Rmu_co8304723.1_g000001
rosa_roxburghii Rroxscaffold_3G00246370
rosa_samantha Rh7AG269800 Rh7BG265200 Rh7CG289000 Rh7DG277900
rosa_wichuraiana Rw7G023440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 93
AccII CGCG 1 cut(s) 271
AciI CCGC 2 cut(s) 81, 488
AcoI YGGCCR 2 cut(s) 55, 489
AcsI RAATTY 1 cut(s) 538
AcuI CTGAAG 1 cut(s) 303
AfaI GTAC 6 cut(s) 23, 77, 321, 360, 370, 446
AfiI CCNNNNNNNGG 1 cut(s) 378
AgsI TTSAA 2 cut(s) 455, 629
AjnI CCWGG 1 cut(s) 181
AluBI AGCT 7 cut(s) 89, 248, 294, 512, 598, 642, 716
AluI AGCT 7 cut(s) 89, 248, 294, 512, 598, 642, 716
Ama87I CYCGRG 1 cut(s) 728
AoxI GGCC 3 cut(s) 55, 389, 489
ApeKI GCWGC 3 cut(s) 245, 512, 713
ApoI RAATTY 1 cut(s) 538
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
Asp700I GAANNNNTTC 1 cut(s) 202
AspLEI GCGC 2 cut(s) 271, 727
AspS9I GGNCC 2 cut(s) 434, 679
AsuHPI GGTGA 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 728
AvaII GGWCC 2 cut(s) 434, 679
BaeI ACNNNNGTAYC 2 cut(s) 469, 502
BbvI GCAGC 3 cut(s) 257, 499, 725
BccI CCATC 6 cut(s) 47, 125, 245, 425, 466, 730
BceAI ACGGC 2 cut(s) 42, 726
BciT130I CCWGG 1 cut(s) 183
BisI GCNGC 5 cut(s) 81, 246, 489, 513, 714
BlsI GCNGC 5 cut(s) 82, 247, 490, 514, 715
Bme1390I CCNGG 1 cut(s) 183
Bme18I GGWCC 2 cut(s) 434, 679
BmeT110I CYCGRG 1 cut(s) 728
BmgT120I GGNCC 2 cut(s) 434, 679
BmiI GGNNCC 2 cut(s) 187, 681
BmrFI CCNGG 1 cut(s) 183
BmrI ACTGGG 1 cut(s) 27
BmsI GCATC 5 cut(s) 165, 173, 304, 679, 730
BmuI ACTGGG 1 cut(s) 27
Bpu10I CCTNAGC 1 cut(s) 386
BsaJI CCNNGG 1 cut(s) 612
BsaXI ACNNNNNCTCC 4 cut(s) 14, 31, 44, 61
Bsc4I CCNNNNNNNGG 1 cut(s) 378
Bse1I ACTGG 1 cut(s) 22
BseBI CCWGG 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 612
BseGI GGATG 1 cut(s) 745
BseLI CCNNNNNNNGG 1 cut(s) 378
BseMII CTCAG 3 cut(s) 330, 400, 537
BseNI ACTGG 1 cut(s) 22
BseRI GAGGAG 2 cut(s) 668, 746
BseXI GCAGC 3 cut(s) 257, 499, 725
BseYI CCCAGC 1 cut(s) 236
Bsh1236I CGCG 1 cut(s) 271
BshFI GGCC 3 cut(s) 57, 391, 491
BsiHKCI CYCGRG 1 cut(s) 728
BslFI GGGAC 2 cut(s) 118, 665
BslI CCNNNNNNNGG 1 cut(s) 378
BsmFI GGGAC 2 cut(s) 118, 665
BsnI GGCC 3 cut(s) 57, 391, 491
BsoBI CYCGRG 1 cut(s) 728
Bsp1407I TGTACA 2 cut(s) 358, 444
Bsp143I GATC 3 cut(s) 25, 69, 688
BspACI CCGC 2 cut(s) 81, 488
BspANI GGCC 3 cut(s) 57, 391, 491
BspCNI CTCAG 3 cut(s) 331, 399, 538
BspFNI CGCG 1 cut(s) 271
BspLI GGNNCC 2 cut(s) 187, 681
BsrGI TGTACA 2 cut(s) 358, 444
BsrI ACTGG 1 cut(s) 22
BssECI CCNNGG 1 cut(s) 612
BssMI GATC 3 cut(s) 25, 69, 688
BssT1I CCWWGG 1 cut(s) 612
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 2 cut(s) 101, 109
BstAUI TGTACA 2 cut(s) 358, 444
BstC8I GCNNGC 4 cut(s) 223, 243, 273, 510
BstDEI CTNAG 4 cut(s) 276, 339, 386, 546
BstF5I GGATG 1 cut(s) 745
BstFNI CGCG 1 cut(s) 271
BstHHI GCGC 2 cut(s) 271, 727
BstKTI GATC 3 cut(s) 28, 72, 691
BstMBI GATC 3 cut(s) 25, 69, 688
BstMWI GCNNNNNNNGC 7 cut(s) 86, 227, 242, 564, 648, 710, 722
BstNI CCWGG 1 cut(s) 183
BstNSI RCATGY 1 cut(s) 558
BstSCI CCNGG 1 cut(s) 181
BstUI CGCG 1 cut(s) 271
BstV1I GCAGC 3 cut(s) 257, 499, 725
BsuRI GGCC 3 cut(s) 57, 391, 491
BtgZI GCGATG 1 cut(s) 327
BtsCI GGATG 1 cut(s) 745
BtsIMutI CAGTG 1 cut(s) 106
Cac8I GCNNGC 4 cut(s) 223, 243, 273, 510
CfoI GCGC 2 cut(s) 271, 727
Cfr13I GGNCC 2 cut(s) 434, 679
CsiI ACCWGGT 1 cut(s) 181
Csp6I GTAC 6 cut(s) 22, 76, 320, 359, 369, 445
CviAII CATG 4 cut(s) 218, 226, 448, 555
CviQI GTAC 6 cut(s) 22, 76, 320, 359, 369, 445
DdeI CTNAG 4 cut(s) 276, 339, 386, 546
DpnI GATC 3 cut(s) 27, 71, 690
DpnII GATC 3 cut(s) 25, 69, 688
EaeI YGGCCR 2 cut(s) 55, 489
Eco130I CCWWGG 1 cut(s) 612
Eco147I AGGCCT 1 cut(s) 391
Eco47I GGWCC 2 cut(s) 434, 679
Eco57I CTGAAG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 728
EcoO109I RGGNCCY 1 cut(s) 679
EcoRII CCWGG 1 cut(s) 181
EcoT14I CCWWGG 1 cut(s) 612
ErhI CCWWGG 1 cut(s) 612
FaeI CATG 4 cut(s) 221, 229, 451, 558
FaqI GGGAC 2 cut(s) 118, 665
FatI CATG 4 cut(s) 217, 225, 447, 554
FblI GTMKAC 1 cut(s) 93
Fnu4HI GCNGC 5 cut(s) 81, 246, 489, 513, 714
Fsp4HI GCNGC 5 cut(s) 81, 246, 489, 513, 714
GlaI GCGC 2 cut(s) 270, 726
GluI GCNGC 5 cut(s) 81, 246, 489, 513, 714
GsaI CCCAGC 1 cut(s) 240
HaeIII GGCC 3 cut(s) 57, 391, 491
HhaI GCGC 2 cut(s) 271, 727
Hin1II CATG 4 cut(s) 221, 229, 451, 558
Hin6I GCGC 2 cut(s) 269, 725
HinP1I GCGC 2 cut(s) 269, 725
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 3 cut(s) 117, 198, 656
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 2 cut(s) 94, 369
Hpy188I TCNGA 4 cut(s) 283, 340, 547, 675
Hpy188III TCNNGA 3 cut(s) 50, 67, 695
Hpy8I GTNNAC 2 cut(s) 94, 369
HpyAV CCTTC 3 cut(s) 322, 557, 670
HpyCH4III ACNGT 2 cut(s) 101, 109
HpyCH4V TGCA 5 cut(s) 126, 164, 245, 350, 558
HpyF10VI GCNNNNNNNGC 7 cut(s) 86, 227, 242, 564, 648, 710, 722
HpyF3I CTNAG 4 cut(s) 276, 339, 386, 546
Hsp92II CATG 4 cut(s) 221, 229, 451, 558
HspAI GCGC 2 cut(s) 269, 725
Kzo9I GATC 3 cut(s) 25, 69, 688
Lsp1109I GCAGC 3 cut(s) 257, 499, 725
LweI GCATC 5 cut(s) 165, 173, 304, 679, 730
MabI ACCWGGT 1 cut(s) 181
MaeIII GTNAC 1 cut(s) 34
MalI GATC 3 cut(s) 27, 71, 690
MboI GATC 3 cut(s) 25, 69, 688
MboII GAAGA 2 cut(s) 683, 710
MfeI CAATTG 1 cut(s) 440
MluCI AATT 6 cut(s) 261, 440, 525, 533, 538, 629
MlyI GAGTC 1 cut(s) 665
MmeI TCCRAC 2 cut(s) 94, 213
MnlI CCTC 6 cut(s) 249, 410, 447, 568, 646, 724
MroXI GAANNNNTTC 1 cut(s) 202
MseI TTAA 1 cut(s) 536
MslI CAYNNNNRTG 1 cut(s) 216
MspA1I CMGCKG 1 cut(s) 248
MspR9I CCNGG 1 cut(s) 183
MunI CAATTG 1 cut(s) 440
MvaI CCWGG 1 cut(s) 183
MvnI CGCG 1 cut(s) 271
MwoI GCNNNNNNNGC 7 cut(s) 86, 227, 242, 564, 648, 710, 722
NdeII GATC 3 cut(s) 25, 69, 688
NlaIII CATG 4 cut(s) 221, 229, 451, 558
NlaIV GGNNCC 2 cut(s) 187, 681
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 1 cut(s) 558
PaeR7I CTCGAG 1 cut(s) 728
PceI AGGCCT 1 cut(s) 391
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PdmI GAANNNNTTC 1 cut(s) 202
PfeI GAWTC 2 cut(s) 117, 198
PkrI GCNGC 5 cut(s) 82, 247, 490, 514, 715
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
PpuMI RGGWCCY 1 cut(s) 679
Psp5II RGGWCCY 1 cut(s) 679
Psp6I CCWGG 1 cut(s) 181
PspFI CCCAGC 1 cut(s) 236
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 2 cut(s) 187, 681
PspPI GGNCC 2 cut(s) 434, 679
PspPPI RGGWCCY 1 cut(s) 679
PspXI VCTCGAGB 1 cut(s) 728
PvuII CAGCTG 1 cut(s) 248
RsaI GTAC 6 cut(s) 23, 77, 321, 360, 370, 446
RsaNI GTAC 6 cut(s) 22, 76, 320, 359, 369, 445
RseI CAYNNNNRTG 1 cut(s) 216
SalI GTCGAC 1 cut(s) 92
SaqAI TTAA 1 cut(s) 536
SatI GCNGC 5 cut(s) 81, 246, 489, 513, 714
Sau3AI GATC 3 cut(s) 25, 69, 688
Sau96I GGNCC 2 cut(s) 434, 679
SchI GAGTC 1 cut(s) 665
ScrFI CCNGG 1 cut(s) 183
SexAI ACCWGGT 1 cut(s) 181
SfaNI GCATC 5 cut(s) 165, 173, 304, 679, 730
Sfr274I CTCGAG 1 cut(s) 728
SinI GGWCC 2 cut(s) 434, 679
SlaI CTCGAG 1 cut(s) 728
SmiMI CAYNNNNRTG 1 cut(s) 216
SmlI CTYRAG 1 cut(s) 728
SmoI CTYRAG 1 cut(s) 728
Sse9I AATT 6 cut(s) 261, 440, 525, 533, 538, 629
SseBI AGGCCT 1 cut(s) 391
SsiI CCGC 2 cut(s) 81, 488
StuI AGGCCT 1 cut(s) 391
StyD4I CCNGG 1 cut(s) 181
StyI CCWWGG 1 cut(s) 612
TaaI ACNGT 2 cut(s) 101, 109
TaqI TCGA 4 cut(s) 72, 93, 573, 729
TasI AATT 6 cut(s) 261, 440, 525, 533, 538, 629
TatI WGTACW 2 cut(s) 358, 444
TauI GCSGC 2 cut(s) 83, 491
TfiI GAWTC 2 cut(s) 117, 198
Tru1I TTAA 1 cut(s) 536
Tru9I TTAA 1 cut(s) 536
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 3 cut(s) 245, 512, 713
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 2 cut(s) 200, 621
TspGWI ACGGA 2 cut(s) 22, 536
TspRI CASTG 1 cut(s) 106
VpaK11BI GGWCC 2 cut(s) 434, 679
XapI RAATTY 1 cut(s) 538
XceI RCATGY 1 cut(s) 558
XhoI CTCGAG 1 cut(s) 728
XmiI GTMKAC 1 cut(s) 93
XmnI GAANNNNTTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.