Rh7AG269800

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0014407
Physical Location & Seq
Reverse (-)
28100108 .. 28103544
3437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG269800.1

Sequence Viewer

Length: 750 bp
ATGAGTAGCATAGGAACAGGCTACGATCTCTCAGTCACCACATTTTCTCCTGATGGCCGTGTATTCCAGATTGAGTATGCAACCAAAGCTGTTGACAACTCCGGGACTGTTATTGGGATCAAATGCAAGGATGGAGTTGTTATGGGTGTGGAAAAGCTCATTGCATCAAAGATGATGCTGCCTGGTTCCAACAGAAGAATTCATTCTGTTCATCGCCACTCTGGCATGGCCGTGGCAGGATTAGCAGCTGATGGCAGGCAGATTGTTGCCAGGACAAAGTCGGAAGCTACCAACTATGAAAATGTGTATGGTGATCAGATTCCTATCAAGGAACTTGCTGAACGTGTTGCCAGTTATGTGCATCTGTGCACACTATATTGGTGGCTCAGACCTTTTGGATGTGGGATCATCCTTGGAGGTTATGACAGAGATGGGCCACAATTGTACATGGTTGAACCTTCTGGTGTTTCTTACAGGTATTTTGGTGCAGCAATTGGGAAGGGAAGGCAGGCTGCTAAAACAGAGATTGAAAAGTTGAAGCTTTCAGAATTGACTTGTCGGCAAGGTGTTATTGAAGTAGCCAAGATCATTTATGGAATACACGATGAGGCGAAGGACAAAGACTTTGAATTGGAAATGAGCTGGGTCTGTGATGAATCGAACCGTCTGCATCAGAAGGTTCCAGATGAGCTTCTAGAGGAAGCAAAGGCAGCGGCAAGAGCAGCATTGGAAGAAATGGATGCAGACTAA

Protein Analysis

249

Amino Acids

27.46

Weight (kDa)

5.75

Isoelectric Point (pI)

37.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome_A_N PF10584 8 - 30 7.8e-14 Proteasome subunit A N-terminal signature
Proteasome PF00227 31 - 216 5.4e-51 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014407)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27020 AT2G27020
fragaria_vesca FvH4_5g23670
malus_domestica MD14G1092100.v1.1
prunus_persica Prupe.7G022800_v2.0.a1 Prupe.7G022800_v2.0.a1
pyrus_communis pycom14g08930 pycom14g08970
rosa_chinensis RchiOBHm_Chr7g0212971
rosa_laevigata RLG00000002841
rosa_multiflora Rmu_co8304723.1_g000001
rosa_roxburghii Rroxscaffold_3G00246370
rosa_samantha Rh7AG269800 Rh7BG265200 Rh7CG289000 Rh7DG277900
rosa_wichuraiana Rw7G023440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 713
AclWI GGATC 2 cut(s) 125, 413
AcoI YGGCCR 2 cut(s) 55, 228
AcsI RAATTY 1 cut(s) 198
AfaI GTAC 1 cut(s) 446
AflIII ACRYGT 1 cut(s) 343
AgsI TTSAA 5 cut(s) 455, 530, 538, 575, 629
AjnI CCWGG 2 cut(s) 181, 269
AluBI AGCT 7 cut(s) 89, 157, 248, 287, 541, 642, 691
AluI AGCT 7 cut(s) 89, 157, 248, 287, 541, 642, 691
Alw21I GWGCWC 1 cut(s) 371
Alw44I GTGCAC 1 cut(s) 367
AlwI GGATC 2 cut(s) 125, 413
AoxI GGCC 3 cut(s) 55, 228, 434
ApaLI GTGCAC 1 cut(s) 367
ApeKI GCWGC 6 cut(s) 178, 245, 488, 512, 710, 722
ApoI RAATTY 1 cut(s) 198
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
Asp700I GAANNNNTTC 1 cut(s) 202
AspS9I GGNCC 1 cut(s) 434
AsuC2I CCSGG 1 cut(s) 103
AsuHPI GGTGA 2 cut(s) 28, 323
BaeGI GKGCMC 1 cut(s) 371
Bbv12I GWGCWC 1 cut(s) 371
BbvI GCAGC 6 cut(s) 165, 257, 499, 500, 722, 734
BccI CCATC 4 cut(s) 47, 125, 245, 425
BceAI ACGGC 2 cut(s) 42, 215
BcgI CGANNNNNNTGC 2 cut(s) 649, 683
BciT130I CCWGG 2 cut(s) 183, 271
BclI TGATCA 1 cut(s) 313
BcnI CCSGG 1 cut(s) 103
BfaI CTAG 1 cut(s) 695
BglI GCCNNNNNGGC 1 cut(s) 222
BisI GCNGC 7 cut(s) 179, 246, 489, 513, 711, 714, 723
BlsI GCNGC 7 cut(s) 180, 247, 490, 514, 712, 715, 724
Bme1390I CCNGG 3 cut(s) 103, 183, 271
BmgT120I GGNCC 1 cut(s) 434
BmiI GGNNCC 2 cut(s) 187, 681
BmrFI CCNGG 3 cut(s) 103, 183, 271
BmsI GCATC 5 cut(s) 165, 173, 370, 679, 730
BpuMI CCSGG 1 cut(s) 103
BsaBI GATNNNNATC 1 cut(s) 323
BsaJI CCNNGG 2 cut(s) 231, 412
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bse1I ACTGG 1 cut(s) 351
Bse3DI GCAATG 1 cut(s) 159
Bse8I GATNNNNATC 1 cut(s) 323
BseBI CCWGG 2 cut(s) 183, 271
BseDI CCNNGG 2 cut(s) 231, 412
BseGI GGATG 4 cut(s) 136, 404, 408, 745
BseJI GATNNNNATC 1 cut(s) 323
BseMI GCAATG 1 cut(s) 159
BseMII CTCAG 2 cut(s) 45, 400
BseNI ACTGG 1 cut(s) 351
BseSI GKGCMC 1 cut(s) 371
BseXI GCAGC 6 cut(s) 165, 257, 499, 500, 722, 734
BseYI CCCAGC 1 cut(s) 642
BsgI GTGCAG 1 cut(s) 507
BshFI GGCC 3 cut(s) 57, 230, 436
BsiHKAI GWGCWC 1 cut(s) 371
BsiSI CCGG 1 cut(s) 102
BslFI GGGAC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 118
BsnI GGCC 3 cut(s) 57, 230, 436
Bsp1286I GDGCHC 1 cut(s) 371
Bsp1407I TGTACA 1 cut(s) 444
Bsp143I GATC 5 cut(s) 25, 117, 313, 405, 585
BspACI CCGC 1 cut(s) 713
BspANI GGCC 3 cut(s) 57, 230, 436
BspCNI CTCAG 2 cut(s) 44, 399
BspLI GGNNCC 2 cut(s) 187, 681
BspPI GGATC 2 cut(s) 125, 413
BsrDI GCAATG 1 cut(s) 159
BsrGI TGTACA 1 cut(s) 444
BsrI ACTGG 1 cut(s) 351
BssECI CCNNGG 2 cut(s) 231, 412
BssMI GATC 5 cut(s) 25, 117, 313, 405, 585
BssT1I CCWWGG 1 cut(s) 412
Bst2UI CCWGG 2 cut(s) 183, 271
Bst4CI ACNGT 2 cut(s) 109, 665
BstAUI TGTACA 1 cut(s) 444
BstC8I GCNNGC 2 cut(s) 257, 510
BstDEI CTNAG 2 cut(s) 31, 386
BstDSI CCRYGG 1 cut(s) 231
BstF5I GGATG 4 cut(s) 136, 404, 408, 745
BstKTI GATC 5 cut(s) 28, 120, 316, 408, 588
BstMBI GATC 5 cut(s) 25, 117, 313, 405, 585
BstMWI GCNNNNNNNGC 6 cut(s) 86, 222, 242, 710, 719, 722
BstNI CCWGG 2 cut(s) 183, 271
BstSCI CCNGG 3 cut(s) 101, 181, 269
BstSLI GKGCMC 1 cut(s) 371
BstV1I GCAGC 6 cut(s) 165, 257, 499, 500, 722, 734
BsuRI GGCC 3 cut(s) 57, 230, 436
BtgI CCRYGG 1 cut(s) 231
BtgZI GCGATG 1 cut(s) 197
BtsCI GGATG 4 cut(s) 136, 404, 408, 745
Cac8I GCNNGC 2 cut(s) 257, 510
Cfr13I GGNCC 1 cut(s) 434
Csp6I GTAC 1 cut(s) 445
CviAII CATG 2 cut(s) 226, 448
CviQI GTAC 1 cut(s) 445
DdeI CTNAG 2 cut(s) 31, 386
DpnI GATC 5 cut(s) 27, 119, 315, 407, 587
DpnII GATC 5 cut(s) 25, 117, 313, 405, 585
EaeI YGGCCR 2 cut(s) 55, 228
Eco130I CCWWGG 1 cut(s) 412
EcoRI GAATTC 1 cut(s) 198
EcoRII CCWGG 2 cut(s) 181, 269
EcoT14I CCWWGG 1 cut(s) 412
ErhI CCWWGG 1 cut(s) 412
FaeI CATG 2 cut(s) 229, 451
FaqI GGGAC 1 cut(s) 118
FatI CATG 2 cut(s) 225, 447
FbaI TGATCA 1 cut(s) 313
Fnu4HI GCNGC 7 cut(s) 179, 246, 489, 513, 711, 714, 723
FokI GGATG 3 cut(s) 143, 395, 411
Fsp4HI GCNGC 7 cut(s) 179, 246, 489, 513, 711, 714, 723
FspBI CTAG 1 cut(s) 695
GluI GCNGC 7 cut(s) 179, 246, 489, 513, 711, 714, 723
GsaI CCCAGC 1 cut(s) 646
HaeIII GGCC 3 cut(s) 57, 230, 436
HapII CCGG 1 cut(s) 102
Hin1II CATG 2 cut(s) 229, 451
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 539
HinfI GANTC 2 cut(s) 319, 656
HpaII CCGG 1 cut(s) 102
HphI GGTGA 2 cut(s) 28, 323
Hpy166II GTNNAC 2 cut(s) 94, 369
Hpy188I TCNGA 5 cut(s) 283, 318, 389, 547, 675
Hpy188III TCNNGA 4 cut(s) 50, 67, 683, 695
Hpy8I GTNNAC 2 cut(s) 94, 369
HpyAV CCTTC 5 cut(s) 468, 493, 498, 607, 670
HpyCH4III ACNGT 2 cut(s) 109, 665
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 8 cut(s) 80, 126, 164, 361, 369, 488, 670, 743
HpyF10VI GCNNNNNNNGC 6 cut(s) 86, 222, 242, 710, 719, 722
HpyF3I CTNAG 2 cut(s) 31, 386
HpySE526I ACGT 1 cut(s) 343
Hsp92II CATG 2 cut(s) 229, 451
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 5 cut(s) 25, 117, 313, 405, 585
Lsp1109I GCAGC 6 cut(s) 165, 257, 499, 500, 722, 734
LweI GCATC 5 cut(s) 165, 173, 370, 679, 730
MaeI CTAG 1 cut(s) 695
MaeII ACGT 1 cut(s) 343
MaeIII GTNAC 1 cut(s) 34
MalI GATC 5 cut(s) 27, 119, 315, 407, 587
MboI GATC 5 cut(s) 25, 117, 313, 405, 585
MboII GAAGA 2 cut(s) 207, 743
MfeI CAATTG 2 cut(s) 440, 492
MhlI GDGCHC 1 cut(s) 371
MluCI AATT 5 cut(s) 198, 440, 492, 548, 629
MmeI TCCRAC 2 cut(s) 213, 261
MnlI CCTC 3 cut(s) 410, 601, 691
MroXI GAANNNNTTC 1 cut(s) 202
MslI CAYNNNNRTG 1 cut(s) 230
MspA1I CMGCKG 2 cut(s) 248, 713
MspI CCGG 1 cut(s) 102
MspR9I CCNGG 3 cut(s) 103, 183, 271
MunI CAATTG 2 cut(s) 440, 492
MvaI CCWGG 2 cut(s) 183, 271
MwoI GCNNNNNNNGC 6 cut(s) 86, 222, 242, 710, 719, 722
NciI CCSGG 1 cut(s) 103
NdeII GATC 5 cut(s) 25, 117, 313, 405, 585
NlaIII CATG 2 cut(s) 229, 451
NlaIV GGNNCC 2 cut(s) 187, 681
NmuCI GTSAC 1 cut(s) 34
PdmI GAANNNNTTC 1 cut(s) 202
PfeI GAWTC 2 cut(s) 319, 656
PflFI GACNNNGTC 1 cut(s) 277
PfoI TCCNGGA 1 cut(s) 101
PkrI GCNGC 7 cut(s) 180, 247, 490, 514, 712, 715, 724
Psp6I CCWGG 2 cut(s) 181, 269
PspFI CCCAGC 1 cut(s) 642
PspGI CCWGG 2 cut(s) 181, 269
PspN4I GGNNCC 2 cut(s) 187, 681
PspPI GGNCC 1 cut(s) 434
PsyI GACNNNGTC 1 cut(s) 277
PvuII CAGCTG 1 cut(s) 248
RsaI GTAC 1 cut(s) 446
RsaNI GTAC 1 cut(s) 445
RseI CAYNNNNRTG 1 cut(s) 230
SatI GCNGC 7 cut(s) 179, 246, 489, 513, 711, 714, 723
Sau3AI GATC 5 cut(s) 25, 117, 313, 405, 585
Sau96I GGNCC 1 cut(s) 434
ScrFI CCNGG 3 cut(s) 103, 183, 271
SduI GDGCHC 1 cut(s) 371
SfaNI GCATC 5 cut(s) 165, 173, 370, 679, 730
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 5 cut(s) 198, 440, 492, 548, 629
SsiI CCGC 1 cut(s) 713
SspMI CTAG 1 cut(s) 695
StyD4I CCNGG 3 cut(s) 101, 181, 269
StyI CCWWGG 1 cut(s) 412
TaaI ACNGT 2 cut(s) 109, 665
TaiI ACGT 1 cut(s) 346
TaqI TCGA 1 cut(s) 659
TasI AATT 5 cut(s) 198, 440, 492, 548, 629
TatI WGTACW 1 cut(s) 444
TauI GCSGC 1 cut(s) 716
TfiI GAWTC 2 cut(s) 319, 656
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 6 cut(s) 178, 245, 488, 512, 710, 722
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 4 cut(s) 191, 200, 312, 669
Tth111I GACNNNGTC 1 cut(s) 277
VneI GTGCAC 1 cut(s) 367
XapI RAATTY 1 cut(s) 198
XbaI TCTAGA 1 cut(s) 694
XmnI GAANNNNTTC 1 cut(s) 202
XspI CTAG 1 cut(s) 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.