AT2G30800

ATP-dependent RNA helicase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015155
Physical Location & Seq
Reverse (-)
13120292 .. 13126813
6522 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G30800.1

Sequence Viewer

Length: 3900 bp
ATGGGGAACAAGAGATTCAGGTCTGATAATAATGCAGGGAAACCAACTTCCGTCGAAGCTACTAGGATTTGGGCGACTAAGGTCATCGAAGACTTTCGAGCCTCTGGGAACGAAGTTTACACGTTTGAGCATAATCTATCAAATAACGAACGTGGAGTGATTCATCAGATGTGCAGGAAAATGGGTATTCAATCGAAAAGTTCTGGGCGTGGGGAACAAAGGCGTCTTTCGATATTCAAAAGTAGGCATAAGAATGGAAACAAGAATGAAGCTAATGAGAAATCAAATAAAGAGAAGCTCAAATGTGTGTCATTTCCGCCGGGAGCAGATGTTATTCTGCAGGAGTTATTTACTCATTACCCACCTTGTGATGGAGATACGGCTGCAACATCGTTTACCAAGTACTCTGGTAACAAGGGTAAACAAGGACAATGGAAAGATGATTTTTTCCGGAAACCACAGATTAGCAGTGAGGAGATTCTAGAAAAAGTGGCATCTTTGAGTTCTAGACTTAAGAAGGATAAAGCTCTTAAGGAGATCACTAAGCTGCGGTCAAAGCTTCCCATCACATCCTTTAAAGACGCAATTACATCTGCAGTGGAATCCAATCAGGTTATTCTCATTTCTGGTGAAACTGGTTGCGGGAAAACTACTCAGGTGCCTCAATATCTTTTAGACCATATGTGGTCAAGCAAAAGAGAAACTTGCAAAATTGTTTGCACCCAACCTCGTCGGATATCCGCCATGTCAGTTTCTGAAAGAATATCTTGTGAAAGAGGTGAAAGCATTGGAGAAAACATTGGTTACAAGGTTCGACTCCAAAGCAAAGGTGGAAGGCACTCATCAGTTGTTTTCTGCACGAATGGTATTCTCCTGAGGGTGCTTGTAGGAAAAGGCTCTGTTAGCAGTGTTTCCGACATAACTCACATCATTGTGGATGAAATCCATGAAAGAGATTGCTATTCTGATTTCATGTTGGCAATTATAAGGGATTTGCTTCCATCAAATCCCCATCTCCGTCTGATTCTCATGAGTGCTACGCTTGATGCTGAGAGATTTTCTGGATATTTTGGAGGTTGCCCGGTTGTCCGTGTCCCTGGATTCACTTATCCAGTGAGAACCTTATATTTGGAGGATGTGCTCTCTATTCTGAAATCAGGTGGAGATAATCATCTAAGCTCTACCAACTTGAGTATATCAGACCACAAGCTTGATTTGACGGATGAGGATAAACTTGCTTTAGATGAAGCAATCATCTTGGCTTGGACAAATGACGAGTTTGATGCCCTCTTAGATCTAGTTTCTTCTAGAGGAAGTCATGAGATCTACAATTATCAGCACCAATCAACCTGGCTAACACCACTAATGGTCTTTGCTGGAAAGGGCAGGATCAGTGATGTCTGCATGCTCCTCTCCTTTGGTGCAGATTGGAGCTTAAAGTCTAAAGATGGTATGACTGCATTAGAATTGGCTGAAGCAGAGAATCAACTAGAAGCTGCTCAAATAATCAGGGAGCATGCAGACAATTCCCAGTCTAATTCTCAGCAAGGACAACAGTTACTTGATAAATACATGGCGACAATCAACCCAGAGCAGGTCGATGTGAGTCTTATACAACAGTTAATGAGGAAAATATGTGGCGATTCAGAAGACGGTGCGATTCTTGTTTTTCTACCTGGATGGGATGATATAAATAAAACAAGACAGAGATTACTTGAGAACCCTTTCTTTGCAGATAGTGCTAAATTCGACATTATATGTCTTCACTCAATGGTTCCAGCAGGGGAACAGAAGAAAGTTTTTAATCGCCCCCCTCCAGGTTGTCGCAAAATTGTGCTTGCGACAAATATTGCTGAATCAGCAGTTACCATTGATGATGTGGTTTATGTGATAGATAGTGGCCGGATGAAGGAAAAGAGCTATGATCCTTATAATAATGTTTCAACTCTCCAATCTTCTTGGGTATCAAAAGCAAATGCAAAACAGCGTCAGGGTCGAGCAGGCCGCTGCCAACCTGGCATTTGTTATCATCTCTATTCTAGACTTCGGGCAGCCTCTATGCCAGATTTTAAAGTTCCTGAAATCAAGAGGATGCCGGTAGAAGAACTCTGCTTACAAGTTAAGATACTTGATCCAAATTGTAAAACAAATGATTTCCTTCAGAAACTGTTGGACCCGCCTGTTGATCAAAGTATTGCAAACGCCTTAAGCATACTACAGGATATTGGTGCCCTGACACCTCAGGAAGAGCTGACAGAACTTGGAGAGAAATTTGGTCATCTCCCAGTTCATCCTCTGATTAGCAAAATGCTATTCTTCGCTGTGTTAGTAAACTGTCTGGATCCAGCACTTACTCTGGCATGTGCAGCAGACTACAAGGAACCATTTACGATGCCCATGTCGCCTGTTGAAAGACAGAAAGCTGCTGCTGCAAAACTTGAACTTGCATCACTTTGTGGAGGTGACAGTGATCACCTTGCAGTTGTTGCTGCCTTTGAGTGCTGGAAAAACGCAAAAGGAAGAGGTCTTTCAGCAGAGTTTTGCTCTCAGTACTTTGTCTCTCCAAGTGCTATGAAGATGTTGGATCAAATGCGTAGCCAACTTGAGTCGGAACTTAAAAGACATGGGATTATTCCTAACGACATTTCAAGTTGTAGCCAAAATTCGCGTGATCCCGGTATACTCCGTGCTGTGCTAGCAGTAGGATTGTATCCTATGGTGGGAAGATTGTGCCCAGCTTTTGGTAATAATCGAAGAACTATAGTAGAGACTGCTAGTGGTGCTAAAGTTCGTGTCCATTCGCTGTCAAACAACTTCAACTTGTCGTCTAAAAAGTATGATGAATCTTTACTCGTTTTCGATGAGATCACTCGTGGAGATGGAGGCATGCACATAAGAAACTGTACTGTTGCTCGTGATCTTCCTTTGTTACTTATCTCAACAGAGATAGCTGTGGCCCCTACAGGGAGCAGTGATTCTGATGATAGTAATGAAGAAGAAGAAGATGATGAAGAAGTAGCAGCTAATACTAATGAAGAAGTAGCAGCTAATACTAATGAAGAAGGAATGGACATACATAAAGAAGAGAGTAGGCGTGGAGCGAAAATGATGTCTTCACCTGAAAATTCAGTCAAGTTAGTAGTTGATCGCTGGCTGCCTTTCAGAACCACAGCCCTTGAAGTTGCTCAAATGTACATTTTGCGGGAACGACTAATGGCTTCTATTTTATTCAAGGTAACACATCCACGGGAACATTTGCCACCTCATCTCGGAGCTTCTATGCATGCAATAGCTGGTATTCTCTCATATGATGGTCATGCAGGACTTTCATGTCCACCGGAGTCTATGGTACCAAAACATTCAAGGACTGAGATGTATGATACCGGTGGATGGGAAGAGAAACCAAACTCTTTCCTTAACTCACTCTTTTGGTCTCTGAGTTTGAAAGAGAACAAGCATCCTAGTCACACGAATAGGAATCAGCAGCATAACTACAACATGGCTCCAACAGAAGCTGCATCAATACCTAGGCAGCAAAACTATAAGCAAAGAAATCCCAAAGCAACCAACAATACCGATTCGGGTAAGAAGAAAGAGAAGATGTTTGTTAATCCCACCAATAGGATTAACCAGCCTGAAGCTGCTTCTACGGGTAAGCCATCAAAGCACAAATCAGCCAACAGTTCGGGTTCGAGTAACAAGAAAGAGAACATGCCTTCTGATCAGGCCTATGGGAATAAGCAACATAACACAGTTCCAAGAGAAGCTGCCGCACCAATGGCGAAGAATCAAAGCAGTAAGAAAACCAAGACCAGGTCAGGCAACAACTCGGATTCGGGTAAGAAGAAAGAACAGTATATTCCAAAGAGGCAACGCGAAGACAAAGCAGAACAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0007292 GO:0007346 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0009994 GO:0010033 GO:0010467 GO:0012505 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019899 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030154 GO:0032392 GO:0032501 GO:0032502 GO:0032504 GO:0032508 GO:0032991 GO:0034097 GO:0034458 GO:0034612 GO:0034641 GO:0035770 GO:0035821 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043900 GO:0043902 GO:0043903 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044788 GO:0044794 GO:0044827 GO:0044829 GO:0045069 GO:0045070 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0048232 GO:0048468 GO:0048477 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048599 GO:0048609 GO:0048856 GO:0048869 GO:0050789 GO:0050792 GO:0050794 GO:0050896 GO:0051276 GO:0051445 GO:0051446 GO:0051702 GO:0051704 GO:0051726 GO:0051728 GO:0051729 GO:0051817 GO:0051851 GO:0060184 GO:0065007 GO:0070035 GO:0070063 GO:0070555 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140097 GO:0140098 GO:1901360 GO:1901363 GO:1903900 GO:1903902 GO:1990247 GO:1990904 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind R3H

Protein Analysis

1299

Amino Acids

144.48

Weight (kDa)

8.25

Isoelectric Point (pI)

51.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
R3H PF01424 28 - 80 1.1e-10 R3H domain
DEAD PF00270 195 - 349 8.8e-12 DEAD/DEAH box helicase
Helicase_C PF00271 543 - 670 2.5e-11 Helicase conserved C-terminal domain
HA2_C PF21010 765 - 827 1.6e-09 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 895 - 982 5.1e-13 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30800 AT2G30800
fragaria_vesca FvH4_6g38870
malus_domestica MD09G1146500.v1.1
prunus_persica Prupe.3G179000_v2.0.a1
pyrus_communis pycom09g06680
rosa_chinensis RchiOBHm_Chr2g0152461
rosa_laevigata RLG00000020635
rosa_multiflora Rmu_sc0005366.1_g000012
rosa_roxburghii Rroxscaffold_2G00095700
rosa_rugosa Rorug02G0432900
rosa_samantha Rh2AG494700 Rh2BG506900 Rh2CG480900 Rh2DG518100
rosa_wichuraiana Rw2G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 986, 1932
AasI GACNNNNNNGTC 1 cut(s) 3330
Acc36I ACCTGC 1 cut(s) 1585
Acc65I GGTACC 1 cut(s) 3349
AccB1I GGYRCC 3 cut(s) 658, 2228, 3349
AccB7I CCANNNNNTGG 1 cut(s) 2741
AccI GTMKAC 1 cut(s) 2680
AccII CGCG 2 cut(s) 2668, 3876
AccIII TCCGGA 1 cut(s) 450
AciI CCGC 8 cut(s) 317, 550, 642, 741, 2005, 2177, 3202, 3771
AclWI GGATC 7 cut(s) 1397, 1919, 2126, 2336, 2349, 2592, 2666
AcoI YGGCCR 1 cut(s) 1900
AcsI RAATTY 4 cut(s) 1745, 2270, 2662, 3124
AcuI CTGAAG 3 cut(s) 1494, 2144, 3657
AcyI GRCGYC 1 cut(s) 223
AdeI CACNNNGTG 2 cut(s) 368, 2456
AfaI GTAC 5 cut(s) 404, 2552, 2905, 3194, 3351
AflII CTTAAG 3 cut(s) 512, 530, 2206
AflIII ACRYGT 1 cut(s) 120
AgeI ACCGGT 1 cut(s) 3383
AjnI CCWGG 6 cut(s) 1096, 1349, 1675, 1816, 2014, 3812
Alw21I GWGCWC 1 cut(s) 1143
Alw26I GTCTC 3 cut(s) 2563, 2762, 3438
AlwI GGATC 7 cut(s) 1397, 1919, 2126, 2336, 2349, 2592, 2666
AlwNI CAGNNNCTG 3 cut(s) 755, 2167, 3515
Aor13HI TCCGGA 1 cut(s) 450
AoxI GGCC 4 cut(s) 1900, 2002, 2955, 3726
ApoI RAATTY 4 cut(s) 1745, 2270, 2662, 3124
ArsI GACNNNNNNTTYG 2 cut(s) 3095, 3127
AsiGI ACCGGT 1 cut(s) 3383
Asp700I GAANNNNTTC 2 cut(s) 93, 1724
Asp718I GGTACC 1 cut(s) 3349
AspA2I CCTAGG 1 cut(s) 3527
AspS9I GGNCC 2 cut(s) 2173, 2956
AsuC2I CCSGG 3 cut(s) 321, 1082, 2676
AsuHPI GGTGA 5 cut(s) 641, 791, 2465, 2474, 3108
AsuNHI GCTAGC 1 cut(s) 2695
AvaII GGWCC 1 cut(s) 2173
AvrII CCTAGG 1 cut(s) 3527
AxyI CCTNAGG 2 cut(s) 875, 2241
BaeGI GKGCMC 2 cut(s) 2233, 2735
BamHI GGATCC 1 cut(s) 2341
BanI GGYRCC 3 cut(s) 658, 2228, 3349
BauI CACGAG 2 cut(s) 2871, 2913
BbsI GAAGAC 5 cut(s) 96, 1656, 1754, 3105, 3885
Bbv12I GWGCWC 1 cut(s) 1143
BceAI ACGGC 1 cut(s) 396
BcgI CGANNNNNNTGC 2 cut(s) 1265, 1299
BciT130I CCWGG 6 cut(s) 1098, 1351, 1677, 1818, 2016, 3814
BciVI GTATCC 1 cut(s) 2721
BclI TGATCA 3 cut(s) 2185, 2470, 3721
BcnI CCSGG 3 cut(s) 321, 1082, 2676
BcoDI GTCTC 3 cut(s) 2563, 2762, 3438
BfmI CTRYAG 5 cut(s) 338, 594, 2216, 2760, 2961
BfrI CTTAAG 3 cut(s) 512, 530, 2206
BfuAI ACCTGC 1 cut(s) 1585
BfuI GTATCC 1 cut(s) 2721
BglI GCCNNNNNGGC 1 cut(s) 2016
BglII AGATCT 2 cut(s) 1294, 1323
BlnI CCTAGG 1 cut(s) 3527
BmcAI AGTACT 2 cut(s) 404, 2552
Bme1390I CCNGG 9 cut(s) 321, 1082, 1098, 1351, 1677, 1818, 2016, 2676, 3814
Bme18I GGWCC 1 cut(s) 2173
BmgT120I GGNCC 2 cut(s) 2173, 2956
BmiI GGNNCC 9 cut(s) 660, 1776, 2175, 2230, 2343, 2382, 2958, 3351, 3504
BmrFI CCNGG 9 cut(s) 321, 1082, 1098, 1351, 1677, 1818, 2016, 2676, 3814
BmrI ACTGGG 2 cut(s) 1525, 2279
BmsI GCATC 8 cut(s) 503, 1036, 1273, 2082, 2382, 2456, 3466, 3527
BmtI GCTAGC 1 cut(s) 2699
BmuI ACTGGG 2 cut(s) 1525, 2279
BoxI GACNNNNGTC 1 cut(s) 80
BpiI GAAGAC 5 cut(s) 96, 1656, 1754, 3105, 3885
BplI GAGNNNNNCTC 2 cut(s) 2528, 2560
BpmI CTGGAG 1 cut(s) 1800
BpuEI CTTGAG 3 cut(s) 1210, 1736, 2624
BpuMI CCSGG 3 cut(s) 321, 1082, 2676
BsaBI GATNNNNATC 1 cut(s) 1170
BsaHI GRCGYC 1 cut(s) 223
BsaI GGTCTC 1 cut(s) 3438
BsaJI CCNNGG 3 cut(s) 1096, 3245, 3527
BsaWI WCCGGW 3 cut(s) 450, 3337, 3383
BsaXI ACNNNNNCTCC 2 cut(s) 315, 345
Bse118I RCCGGY 2 cut(s) 2095, 3383
Bse1I ACTGG 4 cut(s) 640, 1112, 1531, 2285
Bse21I CCTNAGG 2 cut(s) 875, 2241
Bse8I GATNNNNATC 1 cut(s) 1170
BseAI TCCGGA 1 cut(s) 450
BseBI CCWGG 6 cut(s) 1098, 1351, 1677, 1818, 2016, 3814
BseDI CCNNGG 3 cut(s) 1096, 3245, 3527
BseJI GATNNNNATC 1 cut(s) 1170
BseMII CTCAG 8 cut(s) 668, 866, 1041, 1556, 2255, 2561, 3360, 3428
BseNI ACTGG 4 cut(s) 640, 1112, 1531, 2285
BseRI GAGGAG 2 cut(s) 488, 1400
BseSI GKGCMC 2 cut(s) 2233, 2735
BseYI CCCAGC 1 cut(s) 2734
BsgI GTGCAG 4 cut(s) 193, 841, 1443, 2385
Bsh1236I CGCG 2 cut(s) 2668, 3876
BshFI GGCC 4 cut(s) 1902, 2004, 2957, 3728
BshNI GGYRCC 3 cut(s) 658, 2228, 3349
BshTI ACCGGT 1 cut(s) 3383
BsiHKAI GWGCWC 1 cut(s) 1143
BsiSI CCGG 8 cut(s) 320, 451, 1082, 1903, 2096, 2676, 3338, 3384
BslFI GGGAC 1 cut(s) 1079
BsmAI GTCTC 3 cut(s) 2563, 2762, 3438
BsmFI GGGAC 1 cut(s) 1079
BsnI GGCC 4 cut(s) 1902, 2004, 2957, 3728
Bso31I GGTCTC 1 cut(s) 3438
Bsp1286I GDGCHC 3 cut(s) 1143, 2233, 2735
Bsp13I TCCGGA 1 cut(s) 450
Bsp1407I TGTACA 1 cut(s) 3192
BspACI CCGC 8 cut(s) 317, 550, 642, 741, 2005, 2177, 3202, 3771
BspANI GGCC 4 cut(s) 1902, 2004, 2957, 3728
BspCNI CTCAG 8 cut(s) 667, 867, 1042, 1555, 2254, 2560, 3361, 3429
BspEI TCCGGA 1 cut(s) 450
BspFNI CGCG 2 cut(s) 2668, 3876
BspHI TCATGA 2 cut(s) 1029, 1318
BspLI GGNNCC 9 cut(s) 660, 1776, 2175, 2230, 2343, 2382, 2958, 3351, 3504
BspMAI CTGCAG 2 cut(s) 342, 598
BspMI ACCTGC 1 cut(s) 1585
BspOI GCTAGC 1 cut(s) 2699
BspPI GGATC 7 cut(s) 1397, 1919, 2126, 2336, 2349, 2592, 2666
BspQI GCTCTTC 1 cut(s) 2241
BspT107I GGYRCC 3 cut(s) 658, 2228, 3349
BspTI CTTAAG 3 cut(s) 512, 530, 2206
BspTNI GGTCTC 1 cut(s) 3438
BsrFI RCCGGY 2 cut(s) 2095, 3383
BsrGI TGTACA 1 cut(s) 3192
BsrI ACTGG 4 cut(s) 640, 1112, 1531, 2285
BssAI RCCGGY 2 cut(s) 2095, 3383
BssECI CCNNGG 3 cut(s) 1096, 3245, 3527
BssNAI GTATAC 1 cut(s) 2681
BssNI GRCGYC 1 cut(s) 223
BssSI CACGAG 2 cut(s) 2871, 2913
BssT1I CCWWGG 1 cut(s) 3527
Bst1107I GTATAC 1 cut(s) 2681
Bst2BI CACGAG 2 cut(s) 2871, 2913
Bst2UI CCWGG 6 cut(s) 1098, 1351, 1677, 1818, 2016, 3814
Bst6I CTCTTC 4 cut(s) 2241, 2515, 3078, 3390
BstACI GRCGYC 1 cut(s) 223
BstAFI CTTAAG 3 cut(s) 512, 530, 2206
BstAPI GCANNNNNTGC 2 cut(s) 1739, 2486
BstAUI TGTACA 1 cut(s) 3192
BstC8I GCNNGC 8 cut(s) 1406, 1518, 1839, 2002, 2697, 2888, 3152, 3285
BstDSI CCRYGG 1 cut(s) 3245
BstFNI CGCG 2 cut(s) 2668, 3876
BstMAI GTCTC 3 cut(s) 2563, 2762, 3438
BstNI CCWGG 6 cut(s) 1098, 1351, 1677, 1818, 2016, 3814
BstNSI RCATGY 6 cut(s) 1408, 1520, 2364, 2890, 3287, 3715
BstPAI GACNNNNGTC 1 cut(s) 80
BstSCI CCNGG 9 cut(s) 319, 1080, 1096, 1349, 1675, 1816, 2014, 2674, 3812
BstSFI CTRYAG 5 cut(s) 338, 594, 2216, 2760, 2961
BstSLI GKGCMC 2 cut(s) 2233, 2735
BstUI CGCG 2 cut(s) 2668, 3876
BstV2I GAAGAC 5 cut(s) 96, 1656, 1754, 3105, 3885
BstX2I RGATCY 3 cut(s) 1294, 1323, 2341
BstYI RGATCY 3 cut(s) 1294, 1323, 2341
BstZ17I GTATAC 1 cut(s) 2681
Bsu36I CCTNAGG 2 cut(s) 875, 2241
BsuI GTATCC 1 cut(s) 2721
BsuRI GGCC 4 cut(s) 1902, 2004, 2957, 3728
BtgI CCRYGG 1 cut(s) 3245
BtsI GCAGTG 4 cut(s) 475, 603, 913, 2977
BtsIMutI CAGTG 7 cut(s) 475, 603, 913, 1119, 1399, 2473, 2977
BveI ACCTGC 1 cut(s) 1585
Cac8I GCNNGC 8 cut(s) 1406, 1518, 1839, 2002, 2697, 2888, 3152, 3285
CaiI CAGNNNCTG 3 cut(s) 755, 2167, 3515
CciI TCATGA 2 cut(s) 1029, 1318
Cfr10I RCCGGY 2 cut(s) 2095, 3383
Cfr13I GGNCC 2 cut(s) 2173, 2956
CseI GACGC 3 cut(s) 212, 590, 1976
CsiI ACCWGGT 1 cut(s) 3812
Csp6I GTAC 5 cut(s) 403, 2551, 2904, 3193, 3350
CspAI ACCGGT 1 cut(s) 3383
CspCI CAANNNNNGTGG 2 cut(s) 3157, 3192
CviQI GTAC 5 cut(s) 403, 2551, 2904, 3193, 3350
DraI TTTAAA 2 cut(s) 577, 2071
DraIII CACNNNGTG 2 cut(s) 368, 2456
DrdI GACNNNNNNGTC 1 cut(s) 3330
DseDI GACNNNNNNGTC 1 cut(s) 3330
EaeI YGGCCR 1 cut(s) 1900
Eam1104I CTCTTC 4 cut(s) 2241, 2515, 3078, 3390
EarI CTCTTC 4 cut(s) 2241, 2515, 3078, 3390
EciI GGCGGA 2 cut(s) 306, 730
Eco130I CCWWGG 1 cut(s) 3527
Eco147I AGGCCT 1 cut(s) 3728
Eco31I GGTCTC 1 cut(s) 3438
Eco32I GATATC 1 cut(s) 738
Eco47I GGWCC 1 cut(s) 2173
Eco57I CTGAAG 3 cut(s) 1494, 2144, 3657
Eco81I CCTNAGG 2 cut(s) 875, 2241
EcoRII CCWGG 6 cut(s) 1096, 1349, 1675, 1816, 2014, 3812
EcoRV GATATC 1 cut(s) 738
EcoT14I CCWWGG 1 cut(s) 3527
EcoT22I ATGCAT 1 cut(s) 3285
ErhI CCWWGG 1 cut(s) 3527
FalI AAGNNNNNCTT 4 cut(s) 688, 720, 751, 783
FaqI GGGAC 1 cut(s) 1079
FauI CCCGC 3 cut(s) 635, 2184, 3195
FauNDI CATATG 2 cut(s) 681, 3307
FbaI TGATCA 3 cut(s) 2185, 2470, 3721
FblI GTMKAC 1 cut(s) 2680
GsaI CCCAGC 1 cut(s) 2738
GsuI CTGGAG 1 cut(s) 1800
HaeIII GGCC 4 cut(s) 1902, 2004, 2957, 3728
HapII CCGG 8 cut(s) 320, 451, 1082, 1903, 2096, 2676, 3338, 3384
HgaI GACGC 3 cut(s) 212, 590, 1976
Hin1I GRCGYC 1 cut(s) 223
HindIII AAGCTT 2 cut(s) 557, 1208
HpaII CCGG 8 cut(s) 320, 451, 1082, 1903, 2096, 2676, 3338, 3384
HphI GGTGA 5 cut(s) 641, 791, 2465, 2474, 3108
Hpy166II GTNNAC 6 cut(s) 118, 396, 422, 2332, 2681, 3335
Hpy8I GTNNAC 6 cut(s) 118, 396, 422, 2332, 2681, 3335
Hpy99I CGWCG 2 cut(s) 56, 735
HpyAV CCTTC 6 cut(s) 511, 828, 1903, 2168, 3056, 3726
HpyCH4IV ACGT 2 cut(s) 122, 151
HpySE526I ACGT 2 cut(s) 122, 151
Hsp92I GRCGYC 1 cut(s) 223
Kpn2I TCCGGA 1 cut(s) 450
KpnI GGTACC 1 cut(s) 3353
Ksp22I TGATCA 3 cut(s) 2185, 2470, 3721
LguI GCTCTTC 1 cut(s) 2241
LmnI GCTCC 8 cut(s) 323, 1413, 1431, 1513, 2967, 3098, 3272, 3508
LweI GCATC 8 cut(s) 503, 1036, 1273, 2082, 2382, 2456, 3466, 3527
MabI ACCWGGT 1 cut(s) 3812
MaeII ACGT 2 cut(s) 122, 151
MaeIII GTNAC 9 cut(s) 410, 803, 1557, 1864, 2462, 2928, 3235, 3464, 3695
MflI RGATCY 3 cut(s) 1294, 1323, 2341
MhlI GDGCHC 3 cut(s) 1143, 2233, 2735
MlyI GAGTC 4 cut(s) 810, 1615, 2615, 3350
MmeI TCCRAC 6 cut(s) 713, 939, 2151, 2562, 2589, 3530
Mph1103I ATGCAT 1 cut(s) 3285
MroI TCCGGA 1 cut(s) 450
MroXI GAANNNNTTC 2 cut(s) 93, 1724
MslI CAYNNNNRTG 2 cut(s) 252, 932
MspA1I CMGCKG 1 cut(s) 2007
MspCI CTTAAG 3 cut(s) 512, 530, 2206
MspI CCGG 8 cut(s) 320, 451, 1082, 1903, 2096, 2676, 3338, 3384
MspR9I CCNGG 9 cut(s) 321, 1082, 1098, 1351, 1677, 1818, 2016, 2676, 3814
MvaI CCWGG 6 cut(s) 1098, 1351, 1677, 1818, 2016, 3814
MvnI CGCG 2 cut(s) 2668, 3876
NciI CCSGG 3 cut(s) 321, 1082, 2676
NdeI CATATG 2 cut(s) 681, 3307
NheI GCTAGC 1 cut(s) 2695
NlaIV GGNNCC 9 cut(s) 660, 1776, 2175, 2230, 2343, 2382, 2958, 3351, 3504
NmuCI GTSAC 2 cut(s) 2462, 3464
NsiI ATGCAT 1 cut(s) 3285
NspI RCATGY 6 cut(s) 1408, 1520, 2364, 2890, 3287, 3715
PaeI GCATGC 4 cut(s) 1408, 1520, 2890, 3287
PagI TCATGA 2 cut(s) 1029, 1318
PceI AGGCCT 1 cut(s) 3728
PciSI GCTCTTC 1 cut(s) 2241
PdmI GAANNNNTTC 2 cut(s) 93, 1724
PflFI GACNNNGTC 1 cut(s) 3814
PflMI CCANNNNNTGG 1 cut(s) 2741
PinAI ACCGGT 1 cut(s) 3383
PleI GAGTC 4 cut(s) 810, 1614, 2614, 3349
PpsI GAGTC 4 cut(s) 810, 1614, 2614, 3349
PshAI GACNNNNGTC 1 cut(s) 80
PsiI TTATAA 2 cut(s) 986, 1932
Psp6I CCWGG 6 cut(s) 1096, 1349, 1675, 1816, 2014, 3812
PspFI CCCAGC 1 cut(s) 2734
PspGI CCWGG 6 cut(s) 1096, 1349, 1675, 1816, 2014, 3812
PspN4I GGNNCC 9 cut(s) 660, 1776, 2175, 2230, 2343, 2382, 2958, 3351, 3504
PspPI GGNCC 2 cut(s) 2173, 2956
PsrI GAACNNNNNNTAC 4 cut(s) 101, 133, 2097, 2129
PstI CTGCAG 2 cut(s) 342, 598
PstNI CAGNNNCTG 3 cut(s) 755, 2167, 3515
PsuI RGATCY 3 cut(s) 1294, 1323, 2341
PsyI GACNNNGTC 1 cut(s) 3814
RsaI GTAC 5 cut(s) 404, 2552, 2905, 3194, 3351
RsaNI GTAC 5 cut(s) 403, 2551, 2904, 3193, 3350
RseI CAYNNNNRTG 2 cut(s) 252, 932
SapI GCTCTTC 1 cut(s) 2241
Sau96I GGNCC 2 cut(s) 2173, 2956
ScaI AGTACT 2 cut(s) 404, 2552
SchI GAGTC 4 cut(s) 810, 1615, 2615, 3350
ScrFI CCNGG 9 cut(s) 321, 1082, 1098, 1351, 1677, 1818, 2016, 2676, 3814
SduI GDGCHC 3 cut(s) 1143, 2233, 2735
SexAI ACCWGGT 1 cut(s) 3812
SfaNI GCATC 8 cut(s) 503, 1036, 1273, 2082, 2382, 2456, 3466, 3527
SfcI CTRYAG 5 cut(s) 338, 594, 2216, 2760, 2961
SinI GGWCC 1 cut(s) 2173
SmiMI CAYNNNNRTG 2 cut(s) 252, 932
SmlI CTYRAG 6 cut(s) 512, 530, 1189, 1715, 2206, 2603
SmoI CTYRAG 6 cut(s) 512, 530, 1189, 1715, 2206, 2603
SphI GCATGC 4 cut(s) 1408, 1520, 2890, 3287
SseBI AGGCCT 1 cut(s) 3728
SsiI CCGC 8 cut(s) 317, 550, 642, 741, 2005, 2177, 3202, 3771
SspI AATATT 1 cut(s) 1849
StuI AGGCCT 1 cut(s) 3728
StyD4I CCNGG 9 cut(s) 319, 1080, 1096, 1349, 1675, 1816, 2014, 2674, 3812
StyI CCWWGG 1 cut(s) 3527
TaiI ACGT 2 cut(s) 125, 154
TatI WGTACW 4 cut(s) 402, 2550, 2903, 3192
TauI GCSGC 2 cut(s) 2007, 3773
TscAI CASTG 7 cut(s) 475, 603, 913, 1119, 1399, 2473, 2977
TseFI GTSAC 2 cut(s) 2462, 3464
Tsp45I GTSAC 2 cut(s) 2462, 3464
TspGWI ACGGA 5 cut(s) 40, 1007, 1079, 1235, 2675
TspRI CASTG 7 cut(s) 475, 603, 913, 1119, 1399, 2473, 2977
Tth111I GACNNNGTC 1 cut(s) 3814
Van91I CCANNNNNTGG 1 cut(s) 2741
Vha464I CTTAAG 3 cut(s) 512, 530, 2206
VpaK11BI GGWCC 1 cut(s) 2173
XapI RAATTY 4 cut(s) 1745, 2270, 2662, 3124
XbaI TCTAGA 4 cut(s) 481, 506, 1307, 2039
XceI RCATGY 6 cut(s) 1408, 1520, 2364, 2890, 3287, 3715
XcmI CCANNNNNNNNNTGG 2 cut(s) 827, 1876
XmaJI CCTAGG 1 cut(s) 3527
XmiI GTMKAC 1 cut(s) 2680
XmnI GAANNNNTTC 2 cut(s) 93, 1724
ZrmI AGTACT 2 cut(s) 404, 2552
Zsp2I ATGCAT 1 cut(s) 3285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.