FvH4_6g38870

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015155
Physical Location & Seq
Reverse (-)
30760606 .. 30766622
6017 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g38870.t1

Sequence Viewer

Length: 3669 bp
ATGGTAAGAAAGAGGAATAAAGGGGGACCAGCCCGGTCCCCTGTGATTTCAGAAGAAGCCAGGACGCGATTAGATGACGCCCTCCAAAAGTTCATCGATTCCAAAGAACTACAATACATATTTGGGACCGAGCTCACCACCAATGAACGGGCCTACGTGCACGGACAGTGCCTGAAATTGGATTTGAAATCCAAGAGCAGTGGGTCCGGTAAAACTCGGAGGATTCGGGTTTTCAGGCGGTTTAGGGGGGAGGAAAACGTCAATGCTGCGGACATAATCCCCCGGGTTGAGTTTTCGGAAGGCTCAGCAGAAGTTTTGGGGGATTTGTTCATGCGTTACCCTCCCGGAGATGGAGAGGGAGCCGAAGAAAGGATGGGTGAAGGTTGTCAAAATATTCAGAAAGCCCTTCGGAAGAAAGACCCCTTGTTCTGCAGAACTTCAATGGGCCGGGATGAAGTTGTAAAGAAAGCGGAAGCGCATGCTAAGATGAAAAACTCCAAGATTACAGAGGACAGGCTTAAGCTACCAATGGCATCCTATAAGGATGTGGTAACTTCCGCAGTTGATTCTAACCAGGTTGTTCTTATATCTGGTGAAACTGGGTGCGGAAAGACCACACAGGTCCCGCAATATCTTTTGGACCATATGTGGGGTAAGGGTAAGCCATGTAAAATAGTGTGCACTCAACCGAGGCGTATCTCAGCAATATCAGTTGCTGAGAGAATCTCGTATGAAAGAGGGCAGAGTGTTGGCGGGGATATTGGATACAAGATACGGTTGGAAAGCAAAGGTGGGAAGCACTCATCTGTTGTGTTCTGCACAAATGGGATTCTATTGAGGATTCTAATTTCCAGTGGCGGTGGTAGGTCAAAAACAGATGCATCGAATAAAGAAACCAAACGACAGGATCTCTCTGACATAACTCACATTATTATGGACGAAATTCATGAAAGGGATCGCCACTCTGATTTCATGCTGGCTATTCTCAGAGACATGCTTCCTTCACATCCTCATCTGCATCTGATTTTGATGAGTGCAACTGTTGATGCGGAACGCTTTTCACAATATTTTGGTGGCTGCCCAGTTATCCGTGTCCCAGGGTTCACTTATCCTGTCAAAACTTTCTATTTGGAGGATGTATTGTCTATCTTGAGATCTGTTGAAAATAATCACTTAGAAAACCCTGCATTAGATACCTCAACCTCAGCCCATGATTTGTCAGAGGAAGATAAGCTTGCTTTAGATGAAGCAATTAGTTTGGCTTGGTCAGATGATGATTTTGATCCACTTCTAGAATTGGTTTCTACTAAAGGAACCTATAAAGTCTTTGATTACCAGCATTCTGTAACTGGACTTACACCATTAATGGTGTTTGCTGGAAAGGGTAGATTAGATTATGTTTGCATGCTGCTATCCTTTGGTGCAGACTGCCAATTACGGGCCAAGGATGGAACCACTGCATTAGAGTTGGCTGAGCAGCTAGACCAATTAGAATCTGCTCGAATGTTGAAAGAGCATATGGAAAGTGCTCTTACTAACTCCATGGAACAACAAAATTTGCTCGAGAAATATCTCAAATATTTTAATCCCGAGAATATTGACTTCATTCTTATCGAGCAATTGTTAACAAAAATTTGTAGTGATTCAAAAGATGGAGCTATTCTTGTCTTTCTTCCAGGGTGGGATGACATAAATAAAACGCGGGACAGATTACTCATGAATCCATATTTCAGAGATCCTTCCAAGTGTGTGATTATCCCTCTACATTCAAGTGTTCCCGCTGCTGAACAAAAGAAGGTCTTCAGCCGTCCACCTAGTGGTTGCCGAAAAATTGTTCTTTCAACCAATATTGCTGAAACTGCAGTCACCATTGATGATGTGGTTTATGTTATAGACACTGGTCGGATGAAAGAAAAAAATTATGATCCTTTTAATAATGTTTCAACTCTTCAATCTTCTTGGGTGTCGAAAGCGAGTGCCAAGCAGCGACAGGGGCGTGCGGGCCGTTGTCAACCTGGAATCTGTTATCATCTTTACTCTAAACTTCGAGCAGCTACACTGTCAGATTTTCAGGTGCCTGAGATTAGGAGAATGCCAATTGAGGAACTTTGCCTTCAGGTGAAGTTGCTTGATCCGGATTGCAAGATAGACGAGTTCCTAAACAAGACACTGGATCCTCCTCAATCTGAGACCATACAGAATGCGATTTCTGTTCTTCAGGATATTGGGGCTTTGTCGGTGGAGGAAAGCCTTACAGAACTTGGGGAAAAGCTAGGTTCTTTACCTGTTCATCCATTGACGAGCAAGATGCTATTCTTTGCTATACTGATGAACTGTCTCGACCCAGCTTTAACCCTGGCCTGTGCTGCTGACTACAAGGATCCATTCATTCTTCCTATGGAGTCTGAGGAAAAGGAGAAAGCTCTTGATGCTAAGGCTGAACTTTGTTCGTTGTACGGTGGCCATAGTGATCAGCTGGCACTCATAGCAGCATTTGACTGCTGGAAGATTGCACAAGAACAGGGTAGGGAGAGTCGCTTCTGTGCCAGATACTTTGTCTCTGGAAGTACCATGCATATGCTATCTGGTATGCGGAATCAACTCCAAACGGAGCTAATTAGGCTGGGGTTTATTCCTGAGGATGTGTCAAGATGTAGCTTGAATGCACACAATCCAGGTATAATCAACGCTGTCCTGGTGGCTGGTTTGTATCCCATGTTGGGAAGAGTGCTTTCTACTACTAAGGGTGGAAAAAGAAAAGGAACCTTTATAGAGATTCCTAATGGTAGTAATGTTCGTTTGCACACTCGGTCTACTATTTCGAAATTAGAGTTCAATCCACGTGGTGGTCCTCCATTGGTTGTGTATGATGAGATAACCCGTGGGGATGGGGGTTCGAGCATACGAAACTGTAGTGTGGTTGGGCCACTTCCAGTATTATTGCTGGCAGCAGAAATAGCTGTTGCTCCAGCCAATGATGACGAGGGAAGTGATGTTGATGATAATGATCATGGAAGTGATGAAGATGCAATGGAGATTGATTGCAAGTCGGGTGGACAGCACGAAGAGAAGATTATGTCTTTACCTGATAAATCTGTGATGATTATAGTGGATGGGTGGCTTTATTTTCAGTCGAAAGCTCTTGACGTTGCTCAGATATATTGCTTGAGGGAACGCCTGTCTGCGGCAATACTGTTCAAGGTTACTCATGCAAAGGAAGTTTTGCCTCCTGATCTTGGGGAGTCTATTTATGCTATAGCCTGTGTTCTATCTTATGATGGTCAATCTGGGATTCCATTGCAAAAGTCAAACCAGGTTCCCGTCTCAAATGGAACTGTAAAGTTGAGCGATTACCCTCCCAGTGTTCCGTCACCACGGGTTTCCAGAAACAGAATCCGGCCGTCCCCGTCACAAGTTCCAGTTGGAAAGGTAAACGTTAAAGTGAAAAAGAAGAAAGCAGCGGAATTGAAGCAGCCATTAGTTCAGAGTGCTCCCAGGAAGCCTTTACCACAAGCTACTGATGCAAAGTTGAGTAACGACCCATCAATTCAAAGTGTTCCCCCACCACCCGGTTCAAACACAACCTTGCCGCCCCCGTCGCAAGTTCCAGTCCCGGTAGGTCCTGTCGCAGGAAACAAACGGAAGAAGCGCCGGAATCAGGCCGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0007292 GO:0007346 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0009994 GO:0010033 GO:0010467 GO:0012505 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019899 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030154 GO:0032392 GO:0032501 GO:0032502 GO:0032504 GO:0032508 GO:0032991 GO:0034097 GO:0034458 GO:0034612 GO:0034641 GO:0035770 GO:0035821 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043900 GO:0043902 GO:0043903 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044788 GO:0044794 GO:0044827 GO:0044829 GO:0045069 GO:0045070 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0048232 GO:0048468 GO:0048477 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048599 GO:0048609 GO:0048856 GO:0048869 GO:0050789 GO:0050792 GO:0050794 GO:0050896 GO:0051276 GO:0051445 GO:0051446 GO:0051702 GO:0051704 GO:0051726 GO:0051728 GO:0051729 GO:0051817 GO:0051851 GO:0060184 GO:0065007 GO:0070035 GO:0070063 GO:0070555 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140097 GO:0140098 GO:1901360 GO:1901363 GO:1903900 GO:1903902 GO:1990247 GO:1990904 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind R3H

Protein Analysis

1223

Amino Acids

134.97

Weight (kDa)

7.27

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 187 - 349 4.7e-11 DEAD/DEAH box helicase
Ank_2 PF12796 447 - 506 8.5e-06 Ankyrin repeats (3 copies)
Helicase_C PF00271 541 - 670 3.8e-12 Helicase conserved C-terminal domain
HA2_C PF21010 765 - 828 5.5e-10 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 895 - 985 4.1e-09 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30800 AT2G30800
fragaria_vesca FvH4_6g38870
malus_domestica MD09G1146500.v1.1
prunus_persica Prupe.3G179000_v2.0.a1
pyrus_communis pycom09g06680
rosa_chinensis RchiOBHm_Chr2g0152461
rosa_laevigata RLG00000020635
rosa_multiflora Rmu_sc0005366.1_g000012
rosa_roxburghii Rroxscaffold_2G00095700
rosa_rugosa Rorug02G0432900
rosa_samantha Rh2AG494700 Rh2BG506900 Rh2CG480900 Rh2DG518100
rosa_wichuraiana Rw2G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2074
AccB7I CCANNNNNTGG 2 cut(s) 1817, 2846
AccI GTMKAC 1 cut(s) 2813
AccII CGCG 2 cut(s) 67, 1702
AccIII TCCGGA 1 cut(s) 2134
AclI AACGTT 1 cut(s) 3435
AcoI YGGCCR 2 cut(s) 2461, 3398
AcsI RAATTY 3 cut(s) 942, 1555, 1632
AcuI CTGAAG 3 cut(s) 1786, 2099, 2201
AcvI CACGTG 1 cut(s) 2843
AcyI GRCGYC 1 cut(s) 78
AdeI CACNNNGTG 2 cut(s) 1817, 2846
AfaI GTAC 2 cut(s) 2456, 2569
AflII CTTAAG 1 cut(s) 518
Alw21I GWGCWC 5 cut(s) 135, 162, 683, 1531, 3493
Alw26I GTCTC 5 cut(s) 984, 2183, 2342, 2563, 3328
Alw44I GTGCAC 2 cut(s) 158, 679
AlwNI CAGNNNCTG 2 cut(s) 172, 716
Ama87I CYCGRG 3 cut(s) 282, 1562, 1589
Aor13HI TCCGGA 1 cut(s) 2134
AoxI GGCC 9 cut(s) 150, 445, 1440, 2002, 2358, 2461, 2924, 3398, 3660
ApaLI GTGCAC 2 cut(s) 158, 679
ApoI RAATTY 3 cut(s) 942, 1555, 1632
AseI ATTAAT 1 cut(s) 1364
Asp700I GAANNNNTTC 1 cut(s) 1799
AspLEI GCGC 2 cut(s) 478, 3651
AsuC2I CCSGG 7 cut(s) 34, 283, 284, 345, 449, 3570, 3614
AsuHPI GGTGA 6 cut(s) 127, 389, 605, 1858, 2131, 3363
AsuII TTCGAA 1 cut(s) 2822
AvaI CYCGRG 3 cut(s) 282, 1562, 1589
AvaII GGWCC 8 cut(s) 26, 36, 126, 204, 622, 640, 2849, 3620
AxyI CCTNAGG 1 cut(s) 2637
BaeGI GKGCMC 2 cut(s) 162, 683
BalI TGGCCA 1 cut(s) 2463
BamHI GGATCC 2 cut(s) 2173, 2380
BanI GGYRCC 1 cut(s) 2074
BanII GRGCYC 1 cut(s) 135
BbrPI CACGTG 1 cut(s) 2843
BbsI GAAGAC 1 cut(s) 1792
Bbv12I GWGCWC 5 cut(s) 135, 162, 683, 1531, 3493
BbvCI CCTCAGC 1 cut(s) 1204
BccI CCATC 8 cut(s) 344, 367, 1442, 1646, 2882, 3107, 3272, 3550
BceAI ACGGC 3 cut(s) 1791, 1989, 3385
BcgI CGANNNNNNTGC 2 cut(s) 2290, 2324
BciVI GTATCC 2 cut(s) 758, 2721
BclI TGATCA 2 cut(s) 2470, 3007
BcnI CCSGG 7 cut(s) 34, 283, 284, 345, 449, 3570, 3614
BcoDI GTCTC 5 cut(s) 984, 2183, 2342, 2563, 3328
BfaI CTAG 4 cut(s) 1292, 1481, 1815, 2273
BfmI CTRYAG 4 cut(s) 430, 1860, 2911, 3255
BfoI RGCGCY 1 cut(s) 3652
BfrI CTTAAG 1 cut(s) 518
BfuI GTATCC 2 cut(s) 758, 2721
BglII AGATCT 1 cut(s) 1154
BlpI GCTNAGC 2 cut(s) 304, 1473
Bme18I GGWCC 8 cut(s) 26, 36, 126, 204, 622, 640, 2849, 3620
BmeT110I CYCGRG 3 cut(s) 282, 1562, 1589
BmrI ACTGGG 3 cut(s) 609, 1076, 3354
BmsI GCATC 9 cut(s) 542, 868, 890, 1027, 1036, 2298, 2419, 3016, 3511
BmuI ACTGGG 3 cut(s) 609, 1076, 3354
BoxI GACNNNNGTC 1 cut(s) 1899
BpiI GAAGAC 1 cut(s) 1792
BplI GAGNNNNNCTC 2 cut(s) 710, 742
BpmI CTGGAG 1 cut(s) 2952
Bpu10I CCTNAGC 2 cut(s) 1204, 2433
Bpu1102I GCTNAGC 2 cut(s) 304, 1473
Bpu14I TTCGAA 1 cut(s) 2822
BpuEI CTTGAG 2 cut(s) 1171, 3187
BpuMI CCSGG 7 cut(s) 34, 283, 284, 345, 449, 3570, 3614
Bsa29I ATCGAT 1 cut(s) 96
BsaAI YACGTR 2 cut(s) 157, 2843
BsaBI GATNNNNATC 2 cut(s) 1281, 3006
BsaHI GRCGYC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 2183
BsaWI WCCGGW 2 cut(s) 206, 2134
Bse118I RCCGGY 1 cut(s) 3662
Bse21I CCTNAGG 1 cut(s) 2637
Bse3DI GCAATG 2 cut(s) 3036, 3296
Bse8I GATNNNNATC 2 cut(s) 1281, 3006
BseAI TCCGGA 1 cut(s) 2134
BseCI ATCGAT 1 cut(s) 96
BseJI GATNNNNATC 2 cut(s) 1281, 3006
BseMI GCAATG 2 cut(s) 3036, 3296
BseRI GAGGAG 1 cut(s) 2169
BseSI GKGCMC 2 cut(s) 162, 683
BseX3I CGGCCG 1 cut(s) 3398
BseYI CCCAGC 2 cut(s) 2344, 2623
BsgI GTGCAG 2 cut(s) 802, 1443
Bsh1236I CGCG 2 cut(s) 67, 1702
Bsh1285I CGRYCG 1 cut(s) 3401
BshFI GGCC 9 cut(s) 152, 447, 1442, 2004, 2360, 2463, 2926, 3400, 3662
BshNI GGYRCC 1 cut(s) 2074
BshVI ATCGAT 1 cut(s) 96
BsiEI CGRYCG 1 cut(s) 3401
BsiHKAI GWGCWC 5 cut(s) 135, 162, 683, 1531, 3493
BsiHKCI CYCGRG 3 cut(s) 282, 1562, 1589
BslFI GGGAC 8 cut(s) 22, 39, 139, 608, 1079, 1718, 3388, 3596
BsmAI GTCTC 5 cut(s) 984, 2183, 2342, 2563, 3328
BsmBI CGTCTC 1 cut(s) 3328
BsmFI GGGAC 8 cut(s) 22, 39, 139, 608, 1079, 1718, 3388, 3596
BsmI GAATGC 4 cut(s) 1339, 2097, 2206, 2668
BsnI GGCC 9 cut(s) 152, 447, 1442, 2004, 2360, 2463, 2926, 3400, 3662
Bso31I GGTCTC 1 cut(s) 2183
BsoBI CYCGRG 3 cut(s) 282, 1562, 1589
Bsp119I TTCGAA 1 cut(s) 2822
Bsp1286I GDGCHC 5 cut(s) 135, 162, 683, 1531, 3493
Bsp13I TCCGGA 1 cut(s) 2134
Bsp1720I GCTNAGC 2 cut(s) 304, 1473
Bsp19I CCATGG 1 cut(s) 1542
BspANI GGCC 9 cut(s) 152, 447, 1442, 2004, 2360, 2463, 2926, 3400, 3662
BspDI ATCGAT 1 cut(s) 96
BspEI TCCGGA 1 cut(s) 2134
BspFNI CGCG 2 cut(s) 67, 1702
BspHI TCATGA 2 cut(s) 946, 1716
BspMAI CTGCAG 2 cut(s) 434, 1864
BspT104I TTCGAA 1 cut(s) 2822
BspT107I GGYRCC 1 cut(s) 2074
BspTI CTTAAG 1 cut(s) 518
BspTNI GGTCTC 1 cut(s) 2183
BsrDI GCAATG 2 cut(s) 3036, 3296
BsrFI RCCGGY 1 cut(s) 3662
BssAI RCCGGY 1 cut(s) 3662
BssNI GRCGYC 1 cut(s) 78
BssT1I CCWWGG 2 cut(s) 1443, 1542
Bst4CI ACNGT 9 cut(s) 168, 777, 1042, 2061, 2336, 2459, 2912, 3195, 3337
Bst6I CTCTTC 3 cut(s) 1953, 2719, 3060
BstACI GRCGYC 1 cut(s) 78
BstAFI CTTAAG 1 cut(s) 518
BstBAI YACGTR 2 cut(s) 157, 2843
BstBI TTCGAA 1 cut(s) 2822
BstC8I GCNNGC 8 cut(s) 480, 978, 1236, 1406, 1998, 2002, 2478, 2946
BstDSI CCRYGG 3 cut(s) 1542, 2881, 3374
BstENI CCTNNNNNAGG 1 cut(s) 3627
BstFNI CGCG 2 cut(s) 67, 1702
BstH2I RGCGCY 1 cut(s) 3652
BstHHI GCGC 2 cut(s) 478, 3651
BstMAI GTCTC 5 cut(s) 984, 2183, 2342, 2563, 3328
BstMCI CGRYCG 1 cut(s) 3401
BstMWI GCNNNNNNNGC 9 cut(s) 1859, 1993, 2366, 2429, 2486, 2620, 2957, 3521, 3598
BstNSI RCATGY 3 cut(s) 482, 997, 1408
BstPAI GACNNNNGTC 1 cut(s) 1899
BstSFI CTRYAG 4 cut(s) 430, 1860, 2911, 3255
BstSLI GKGCMC 2 cut(s) 162, 683
BstUI CGCG 2 cut(s) 67, 1702
BstV2I GAAGAC 1 cut(s) 1792
BstX2I RGATCY 5 cut(s) 907, 1154, 1735, 2173, 2380
BstYI RGATCY 5 cut(s) 907, 1154, 1735, 2173, 2380
BstZI CGGCCG 1 cut(s) 3398
Bsu15I ATCGAT 1 cut(s) 96
Bsu36I CCTNAGG 1 cut(s) 2637
BsuI GTATCC 2 cut(s) 758, 2721
BsuRI GGCC 9 cut(s) 152, 447, 1442, 2004, 2360, 2463, 2926, 3400, 3662
BsuTUI ATCGAT 1 cut(s) 96
BtgI CCRYGG 3 cut(s) 1542, 2881, 3374
BtsI GCAGTG 2 cut(s) 205, 1455
BtsIMutI CAGTG 8 cut(s) 173, 205, 859, 1455, 1896, 2057, 2168, 3367
Cac8I GCNNGC 8 cut(s) 480, 978, 1236, 1406, 1998, 2002, 2478, 2946
CaiI CAGNNNCTG 2 cut(s) 172, 716
CciI TCATGA 2 cut(s) 946, 1716
CfoI GCGC 2 cut(s) 478, 3651
Cfr10I RCCGGY 1 cut(s) 3662
Cfr9I CCCGGG 1 cut(s) 282
ClaI ATCGAT 1 cut(s) 96
CseI GACGC 2 cut(s) 73, 86
CsiI ACCWGGT 2 cut(s) 573, 3312
Csp6I GTAC 2 cut(s) 2455, 2568
CspCI CAANNNNNGTGG 6 cut(s) 181, 216, 2824, 2859, 3034, 3069
CviQI GTAC 2 cut(s) 2455, 2568
DraIII CACNNNGTG 2 cut(s) 1817, 2846
EaeI YGGCCR 2 cut(s) 2461, 3398
EagI CGGCCG 1 cut(s) 3398
Eam1104I CTCTTC 3 cut(s) 1953, 2719, 3060
EarI CTCTTC 3 cut(s) 1953, 2719, 3060
Ecl136II GAGCTC 1 cut(s) 133
EclXI CGGCCG 1 cut(s) 3398
Eco130I CCWWGG 2 cut(s) 1443, 1542
Eco24I GRGCYC 1 cut(s) 135
Eco31I GGTCTC 1 cut(s) 2183
Eco47I GGWCC 8 cut(s) 26, 36, 126, 204, 622, 640, 2849, 3620
Eco52I CGGCCG 1 cut(s) 3398
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 3 cut(s) 1786, 2099, 2201
Eco72I CACGTG 1 cut(s) 2843
Eco81I CCTNAGG 1 cut(s) 2637
Eco88I CYCGRG 3 cut(s) 282, 1562, 1589
EcoICRI GAGCTC 1 cut(s) 133
EcoNI CCTNNNNNAGG 1 cut(s) 3627
EcoO109I RGGNCCY 2 cut(s) 622, 3620
EcoT14I CCWWGG 2 cut(s) 1443, 1542
EcoT22I ATGCAT 2 cut(s) 883, 2577
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 2 cut(s) 1443, 1542
Esp3I CGTCTC 1 cut(s) 3328
FalI AAGNNNNNCTT 4 cut(s) 1218, 1250, 1784, 1816
FaqI GGGAC 8 cut(s) 22, 39, 139, 608, 1079, 1718, 3388, 3596
FauI CCCGC 5 cut(s) 633, 746, 1695, 1786, 1993
FauNDI CATATG 3 cut(s) 645, 1518, 2577
FbaI TGATCA 2 cut(s) 2470, 3007
FblI GTMKAC 1 cut(s) 2813
FriOI GRGCYC 1 cut(s) 135
FspBI CTAG 4 cut(s) 1292, 1481, 1815, 2273
GlaI GCGC 2 cut(s) 477, 3650
GsaI CCCAGC 2 cut(s) 2348, 2627
GsuI CTGGAG 1 cut(s) 2952
HaeII RGCGCY 1 cut(s) 3652
HaeIII GGCC 9 cut(s) 152, 447, 1442, 2004, 2360, 2463, 2926, 3400, 3662
HgaI GACGC 2 cut(s) 73, 86
HhaI GCGC 2 cut(s) 478, 3651
Hin1I GRCGYC 1 cut(s) 78
Hin6I GCGC 2 cut(s) 476, 3649
HinP1I GCGC 2 cut(s) 476, 3649
HincII GTYRAC 2 cut(s) 1626, 2012
HindII GTYRAC 2 cut(s) 1626, 2012
HindIII AAGCTT 1 cut(s) 1232
HpaI GTTAAC 1 cut(s) 1626
HphI GGTGA 6 cut(s) 127, 389, 605, 1858, 2131, 3363
Hpy166II GTNNAC 9 cut(s) 160, 681, 1104, 1626, 1811, 2012, 2814, 3056, 3433
Hpy8I GTNNAC 9 cut(s) 160, 681, 1104, 1626, 1811, 2012, 2814, 3056, 3433
Hpy99I CGWCG 1 cut(s) 3601
HpyAV CCTTC 7 cut(s) 293, 374, 416, 1011, 1749, 1789, 2123
HpyCH4III ACNGT 9 cut(s) 168, 777, 1042, 2061, 2336, 2459, 2912, 3195, 3337
HpyCH4IV ACGT 5 cut(s) 156, 258, 2842, 3147, 3435
HpyF10VI GCNNNNNNNGC 9 cut(s) 1859, 1993, 2366, 2429, 2486, 2620, 2957, 3521, 3598
HpySE526I ACGT 5 cut(s) 156, 258, 2842, 3147, 3435
Hsp92I GRCGYC 1 cut(s) 78
HspAI GCGC 2 cut(s) 476, 3649
Kpn2I TCCGGA 1 cut(s) 2134
Ksp22I TGATCA 2 cut(s) 2470, 3007
KspAI GTTAAC 1 cut(s) 1626
LmnI GCTCC 5 cut(s) 359, 1655, 2611, 2971, 3496
LweI GCATC 9 cut(s) 542, 868, 890, 1027, 1036, 2298, 2419, 3016, 3511
MabI ACCWGGT 2 cut(s) 573, 3312
MaeI CTAG 4 cut(s) 1292, 1481, 1815, 2273
MaeII ACGT 5 cut(s) 156, 258, 2842, 3147, 3435
MaeIII GTNAC 8 cut(s) 335, 550, 1345, 1864, 3202, 3369, 3408, 3533
MfeI CAATTG 2 cut(s) 1619, 2097
MflI RGATCY 5 cut(s) 907, 1154, 1735, 2173, 2380
MhlI GDGCHC 5 cut(s) 135, 162, 683, 1531, 3493
MlsI TGGCCA 1 cut(s) 2463
MluNI TGGCCA 1 cut(s) 2463
MlyI GAGTC 3 cut(s) 2411, 2542, 3251
MmeI TCCRAC 3 cut(s) 759, 1883, 3403
Mox20I TGGCCA 1 cut(s) 2463
Mph1103I ATGCAT 2 cut(s) 883, 2577
MroI TCCGGA 1 cut(s) 2134
MroXI GAANNNNTTC 1 cut(s) 1799
MscI TGGCCA 1 cut(s) 2463
MseI TTAA 8 cut(s) 519, 1364, 1584, 1625, 1932, 2351, 3438, 3667
MslI CAYNNNNRTG 4 cut(s) 932, 1770, 2576, 3015
Msp20I TGGCCA 1 cut(s) 2463
MspA1I CMGCKG 3 cut(s) 1781, 2476, 3461
MspCI CTTAAG 1 cut(s) 518
MunI CAATTG 2 cut(s) 1619, 2097
Mva1269I GAATGC 4 cut(s) 1339, 2097, 2206, 2668
MvnI CGCG 2 cut(s) 67, 1702
MwoI GCNNNNNNNGC 9 cut(s) 1859, 1993, 2366, 2429, 2486, 2620, 2957, 3521, 3598
NciI CCSGG 7 cut(s) 34, 283, 284, 345, 449, 3570, 3614
NcoI CCATGG 1 cut(s) 1542
NdeI CATATG 3 cut(s) 645, 1518, 2577
NmuCI GTSAC 3 cut(s) 1864, 3369, 3408
NsiI ATGCAT 2 cut(s) 883, 2577
NspI RCATGY 3 cut(s) 482, 997, 1408
NspV TTCGAA 1 cut(s) 2822
PaeI GCATGC 2 cut(s) 482, 1408
PaeR7I CTCGAG 1 cut(s) 1562
PagI TCATGA 2 cut(s) 946, 1716
PasI CCCWGGG 1 cut(s) 1097
PctI GAATGC 4 cut(s) 1339, 2097, 2206, 2668
PdmI GAANNNNTTC 1 cut(s) 1799
PflMI CCANNNNNTGG 2 cut(s) 1817, 2846
PfoI TCCNGGA 1 cut(s) 343
PleI GAGTC 3 cut(s) 2410, 2541, 3250
PmaCI CACGTG 1 cut(s) 2843
PmlI CACGTG 1 cut(s) 2843
PpsI GAGTC 3 cut(s) 2410, 2541, 3250
Ppu21I YACGTR 2 cut(s) 157, 2843
PpuMI RGGWCCY 2 cut(s) 622, 3620
PshAI GACNNNNGTC 1 cut(s) 1899
PshBI ATTAAT 1 cut(s) 1364
Psp124BI GAGCTC 1 cut(s) 135
Psp1406I AACGTT 1 cut(s) 3435
Psp5II RGGWCCY 2 cut(s) 622, 3620
PspCI CACGTG 1 cut(s) 2843
PspFI CCCAGC 2 cut(s) 2344, 2623
PspPPI RGGWCCY 2 cut(s) 622, 3620
PstI CTGCAG 2 cut(s) 434, 1864
PstNI CAGNNNCTG 2 cut(s) 172, 716
PsuI RGATCY 5 cut(s) 907, 1154, 1735, 2173, 2380
PvuII CAGCTG 1 cut(s) 2476
RsaI GTAC 2 cut(s) 2456, 2569
RsaNI GTAC 2 cut(s) 2455, 2568
RseI CAYNNNNRTG 4 cut(s) 932, 1770, 2576, 3015
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 8 cut(s) 519, 1364, 1584, 1625, 1932, 2351, 3438, 3667
SchI GAGTC 3 cut(s) 2411, 2542, 3251
SduI GDGCHC 5 cut(s) 135, 162, 683, 1531, 3493
SexAI ACCWGGT 2 cut(s) 573, 3312
SfaNI GCATC 9 cut(s) 542, 868, 890, 1027, 1036, 2298, 2419, 3016, 3511
SfcI CTRYAG 4 cut(s) 430, 1860, 2911, 3255
Sfr274I CTCGAG 1 cut(s) 1562
SfuI TTCGAA 1 cut(s) 2822
SinI GGWCC 8 cut(s) 26, 36, 126, 204, 622, 640, 2849, 3620
SlaI CTCGAG 1 cut(s) 1562
SmaI CCCGGG 1 cut(s) 284
SmiMI CAYNNNNRTG 4 cut(s) 932, 1770, 2576, 3015
SmlI CTYRAG 4 cut(s) 518, 1150, 1562, 3166
SmoI CTYRAG 4 cut(s) 518, 1150, 1562, 3166
SphI GCATGC 2 cut(s) 482, 1408
SspI AATATT 5 cut(s) 394, 1067, 1580, 1597, 1849
SspMI CTAG 4 cut(s) 1292, 1481, 1815, 2273
SstI GAGCTC 1 cut(s) 135
StyI CCWWGG 2 cut(s) 1443, 1542
TaaI ACNGT 9 cut(s) 168, 777, 1042, 2061, 2336, 2459, 2912, 3195, 3337
TaiI ACGT 5 cut(s) 159, 261, 2845, 3150, 3438
TaqII GACCGA 2 cut(s) 143, 2799
TauI GCSGC 2 cut(s) 3188, 3592
Tru1I TTAA 8 cut(s) 519, 1364, 1584, 1625, 1932, 2351, 3438, 3667
Tru9I TTAA 8 cut(s) 519, 1364, 1584, 1625, 1932, 2351, 3438, 3667
TscAI CASTG 8 cut(s) 173, 205, 859, 1462, 1903, 2064, 2175, 3367
TseFI GTSAC 3 cut(s) 1864, 3369, 3408
Tsp45I GTSAC 3 cut(s) 1864, 3369, 3408
TspGWI ACGGA 5 cut(s) 177, 1079, 2624, 3357, 3655
TspMI CCCGGG 1 cut(s) 282
TspRI CASTG 8 cut(s) 173, 205, 859, 1462, 1903, 2064, 2175, 3367
Van91I CCANNNNNTGG 2 cut(s) 1817, 2846
Vha464I CTTAAG 1 cut(s) 518
VneI GTGCAC 2 cut(s) 158, 679
VpaK11BI GGWCC 8 cut(s) 26, 36, 126, 204, 622, 640, 2849, 3620
VspI ATTAAT 1 cut(s) 1364
XagI CCTNNNNNAGG 1 cut(s) 3627
XapI RAATTY 3 cut(s) 942, 1555, 1632
XbaI TCTAGA 1 cut(s) 1291
XceI RCATGY 3 cut(s) 482, 997, 1408
XcmI CCANNNNNNNNNTGG 1 cut(s) 1876
XhoI CTCGAG 1 cut(s) 1562
XmaI CCCGGG 1 cut(s) 282
XmiI GTMKAC 1 cut(s) 2813
XmnI GAANNNNTTC 1 cut(s) 1799
XspI CTAG 4 cut(s) 1292, 1481, 1815, 2273
Zsp2I ATGCAT 2 cut(s) 883, 2577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.