MD09G1146500.v1.1

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015155
Physical Location & Seq
Forward (+)
11423875 .. 11433956
10082 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1146500.v1.1.491

Sequence Viewer

Length: 3834 bp
ATGGCGAGGAAGAAGAAACATACTAAAGTTGATGAACCCACAAAGAACCGATTACTTGATGCACTCGAGCGGTTCAACGCTTCAACTGATGAAGTTTACACATTCGAGGCCGGTCTTTCGAGCTTCGAACGGGCTTTCGTGCATGAACAATGCAGGAGATGGTCTATGAAATCGAAGAGTAAAGGGAAAGGGGAACACCGGTGTATCTCTGTTTCCAAAATTAGAAAGAAGAACACTGATGTACCAAGTCTCAGTTCCTTGACATTTTCCGAAGGCACAAAAGAAGTATTAAGTGACTTGTTCGTACATTACCCACCTGGTGATGAAGTGGAGGACTTAGAATTGTCTGGGAAAAGTAGCAATAAGAATGAGAGTGCCCAGCGGAAGAGGGACAATACGTTTCGCAAGCCTTTGATGAACAAGGAAGAAATTGCAAAGAAAGTGGAATCACTTGCTTCTAAATTAAAGAATAACGCTAACTTGAGAAAGATTTTTGAAGACAAGTCCAAGCTTCCAATTATGTCCTACAAGGATGTCATTACATCGACAGTTGAATCTCACCAGGTAATTCTTATATCTGGTGAGACTGGGTGCGGAAAAACAACACAGGTCCCACAATTTATTTTGGACCACATGTGGGGCAAGGGTGAGGCATGTAAAATAGTGTGTACTCAACCTCGACGTATCTCAGCCACTTCAGTTGCTGAGAGAGTCTCTCAGGAAAGAGGAGAAAATGTCGGAGATGATATTGGATACAAGATACGGTTGGAAAGTAAAGCTGGGAGGCAATCATCAATTGTTTTCTGCACAAATGGAATTTTATTGAGGATGCTAGTTTCCAGAGGTGCTGATAGATCAAAAGCAGATGCCTCAAACAAAGATACCGAGCATAATCTTGCTGACATAACTCACATTATAGTGGATGAAATTCATGAAAGGGATCGCTACGCTGATTTGATGCTAGCAATTATCAGAGACATGCTTTCTTCACACCCTCATCTACATCTGATATTAATGAGTGCGACTGTTGATGCTGAACGATTTTCAAAATATTTTGGTGGCTGCCCAATTATCCGTGTTCCTGGGTTCACTTATCCTGTTAAAAGTTTCTATTTAGAGGATGTACTTTCTATCCTGAAATCTGCAGAACATAATCACATTGATAACACTGTAGTAGGTTTACAAACTGAAGACCCTGACTTAACCGAGGAAGATAAGCTTGCTTTAGATGAAGCTATTAATTTGGCTTGGTCTAATGATGAGTTTGATCGCCTTCTAGAATTGCTTTCTACTGAAGGAACACACAAAGTCTTTGATTACCAGCATTCTTTAACCGGACTTACGCCGTTAATGGTGTTTGCTGGAAAGGGTAGAACTGCATATGTTTGCATGCTTTTATCTTTTGCTGCTGACTGCCATTTACGGGCCAAGGATGGAACAACCGCATTGGAGTTGGCTGAACGGGAAAACCAGCAAGAAATTGTTGAGATATTGAAAGAGCATATAGAAAATTCTCTTTCTAACTCAATGAAAGAACAACTGTTACTTGATAAATATCTCCAATATTTCAATCCTGAAAATATTGATGTGGTTCTTATAGAGCAATTATTAAAAAAAATTTGTAATGATTCAAAAGATGGAGCCATCCTTGTTTTCCTTCCAGGGTGGGACGATATAAATAAAACCCGGGAGAGATTACTTATGAATCCACATTTCAGAGATTCTTCAAGGTTTCTGGTAATAGCTCTACATTCGATGGTTCCATCTTTTGAGCAAAAGAAGGTCTTCAAACGTCCCCCTCACGGTTGCCGTAAAATTGTTCTTTCAACTAATATGGCTGAAACTGCCATCACCATAGATGATGTTGTTTATGTTATAGACACTGGTCGGATGAAAGAAAAAAATTATGATCCTTATAGTAATGTATCAACTCTTCAGTCTTCTTGGGTGTCAAAGGCAAATGCCAAGCAGCGAGAGGGCCGAGCAGGCCGTTGCCAGCCTGGAATCTGTTATCATCTCTATTCTAGACTTCGAGAAGCTTCATTGCCAAACTTTCAGGAGCCTGAGATTAAGAGAATGCCAATTGAGGAGCTCTGTCTTCAGGTGAAGTTACTCGACCCAGAATGCAAGGTAGAAGAATTCTTGCGCAAGACATTGGATCCCCCACGTGCAGAGACCATACGCAATGCAGTTTCTGTTCTTCAAGATATTGGGGCACTGTCACTCGATGAGAAACTTACAGATCTTGGAGAGAAGTTAGGCACTCTACCTGTTCATCCACTGACGAGCAGGATGCTTTTCTTTGCAATACTGATGAACTGTCTAGACCCAGCTTTGACTCTGGCATGTGCTTCTGACTACAGGGATCCATTTGCCCTGCCTATGTTACCTGATGAAAGGACAAGAGCTGCTGCTGCTAAATTCGAACTTGCTTCATTGTATGGTGGCCTTAGTGATCAGCTAGCAGTCATTGCAGCATTTGACTGCTGGAAGGCTGCAAAAGAAAGGGGTCAAGAGAGAGATTTCTGTTCTCGATACTTTGTGTCTTCAAGCACGATGCACATGCTATCTGGTATGCGTGGGCAGCTCCAAAAGGAGCTAAGTCGGCATGGGTTTATTCCCGAGGATGTATCAAGATGTAGCTTGAATGCAAGGAACCCAGGTATACTCCGTGCTGTTCTTGTGGCCGGTTTGTATCCAATGGTGGGTAGATTGCTTCCTCCTCTCAAGAATGGGAAACGAACTTTAGTAGAGACTGCTAGTGGTGGTAGAGTTCAATTGCACGTCCACTCAATTAACACTAAGTTATCATACAAGGCAAATCCTGGGGAACGTCCTTTGATTGTTTATGATGAGGTAACTCGCGGGGATGGGGGTATGAATATACGGAACTGCACTGTTGTCGCACCACTTCCATTATTATTGCTTTCATCAGAAATTGCTGTTGCTCCAGCCGATGATGATGATGACAATGACGAGGGGAGTGATGTGGAGTACATAGAGGATGGAAGTGATGAAGATGTAATGGAAATAGATCGTAAGTCAGGTGGACAACGCGAGGAGAAAATTATGTCGTCTCCTGATAAATCAGTCAAAGTTATTGTCGATTGTTGGCTTCATTTTGGGTCCAAAGCTCTTGATGTTGCTCAAGTTTACTGCTTGAGAGAACGCCTATCTACAGCAATCTTATTCAAAGTTACGCATCCAAGGAAAGATTTGCCTCCTGCTCTCGGGGCCTCTGTCTATGCTATCGCATGTGCTCTATCTTATGATGGTCTATCTGGGATCACATTACCGGAGGAATCTGTGGAGTCACCGACTTCTGTGCTGGAGAGTCAAGCGTACTTGCAATTTCTTATCAGCGGTAACTCACATGACCAACACATCCTCCGACCACACTCTAAACCAACACGTAACACAGGCCATAAACTTTCTTACTCTCCCAAACCATGTGAGGGGCATGGAACATCACTTATGAGCCATAACGCACACCAAACACATCAGTCTGCACTTGTGAGCCATGGGACACACCAAACACACCTCAGTCCACATGAGAGGCCTCCACCACAAGGTACTAGTGTACTTGCCGCTAATGGAGCAGTGAGGTCTTATGTGAAAGGAAACAAAGCAGCCAACTTGAGTGGCCCTTCAAGTCACAGCGTTGGACCACCGCTTCATTCGGCTTCCATGACTGCACACCAGAAGCCTCCATCAGAAGGTACTAGGTTACCTGGCATTAATGGAGGGGCAAAGGTGAATGAGCCATTAAGTCAGACTGTTGGCCAACCAGTTAAGTCAACTTCGAGGAAGAAGCGTAGGAATGGTGCCGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0007292 GO:0007346 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0009994 GO:0010033 GO:0010467 GO:0012505 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019899 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030154 GO:0032392 GO:0032501 GO:0032502 GO:0032504 GO:0032508 GO:0032991 GO:0034097 GO:0034458 GO:0034612 GO:0034641 GO:0035770 GO:0035821 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043900 GO:0043902 GO:0043903 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044788 GO:0044794 GO:0044827 GO:0044829 GO:0045069 GO:0045070 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0048232 GO:0048468 GO:0048477 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048599 GO:0048609 GO:0048856 GO:0048869 GO:0050789 GO:0050792 GO:0050794 GO:0050896 GO:0051276 GO:0051445 GO:0051446 GO:0051702 GO:0051704 GO:0051726 GO:0051728 GO:0051729 GO:0051817 GO:0051851 GO:0060184 GO:0065007 GO:0070035 GO:0070063 GO:0070555 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140097 GO:0140098 GO:1901360 GO:1901363 GO:1903900 GO:1903902 GO:1990247 GO:1990904 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind R3H

Protein Analysis

1278

Amino Acids

141.96

Weight (kDa)

7.14

Isoelectric Point (pI)

42.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
R3H PF01424 18 - 73 1.2e-10 R3H domain
DEAD PF00270 179 - 344 9.5e-12 DEAD/DEAH box helicase
Ank_2 PF12796 424 - 501 4.8e-07 Ankyrin repeats (3 copies)
Helicase_C PF00271 536 - 665 6.9e-12 Helicase conserved C-terminal domain
HA2_C PF21010 760 - 845 1.1e-08 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 890 - 979 4.2e-11 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30800 AT2G30800
fragaria_vesca FvH4_6g38870
malus_domestica MD09G1146500.v1.1
prunus_persica Prupe.3G179000_v2.0.a1
pyrus_communis pycom09g06680
rosa_chinensis RchiOBHm_Chr2g0152461
rosa_laevigata RLG00000020635
rosa_multiflora Rmu_sc0005366.1_g000012
rosa_roxburghii Rroxscaffold_2G00095700
rosa_rugosa Rorug02G0432900
rosa_samantha Rh2AG494700 Rh2BG506900 Rh2CG480900 Rh2DG518100
rosa_wichuraiana Rw2G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2147
AccB1I GGYRCC 1 cut(s) 3824
AccBSI CCGCTC 1 cut(s) 70
AccI GTMKAC 1 cut(s) 2665
AccII CGCG 2 cut(s) 2865, 3057
AciI CCGC 8 cut(s) 70, 382, 594, 1443, 2865, 3363, 3588, 3671
AclWI GGATC 7 cut(s) 948, 1904, 2153, 2166, 2360, 2373, 3293
AcoI YGGCCR 2 cut(s) 2685, 3781
AcsI RAATTY 6 cut(s) 816, 927, 1510, 1617, 2138, 2420
AcuI CTGAAG 5 cut(s) 681, 1209, 1314, 1919, 2084
AcvI CACGTG 1 cut(s) 2168
AdeI CACNNNGTG 1 cut(s) 320
AfaI GTAC 9 cut(s) 243, 306, 670, 1125, 2996, 3344, 3574, 3582, 3721
AfiI CCNNNNNNNGG 8 cut(s) 637, 1423, 1802, 2705, 3230, 3455, 3569, 3716
AflIII ACRYGT 2 cut(s) 633, 3410
AgeI ACCGGT 1 cut(s) 198
AhlI ACTAGT 1 cut(s) 3575
AjiI CACGTC 1 cut(s) 2785
AjnI CCWGG 8 cut(s) 316, 561, 1081, 1660, 1999, 2659, 2824, 3730
AjuI GAANNNNNNNTTGG 2 cut(s) 238, 270
Alw21I GWGCWC 2 cut(s) 2094, 3262
Alw26I GTCTC 7 cut(s) 254, 578, 718, 969, 2168, 2747, 3081
AlwI GGATC 7 cut(s) 948, 1904, 2153, 2166, 2360, 2373, 3293
AlwNI CAGNNNCTG 2 cut(s) 704, 2195
Ama87I CYCGRG 4 cut(s) 65, 1686, 2621, 3230
ApoI RAATTY 6 cut(s) 816, 927, 1510, 1617, 2138, 2420
ArsI GACNNNNNNTTYG 2 cut(s) 3087, 3119
AseI ATTAAT 3 cut(s) 1013, 1239, 3738
AsiGI ACCGGT 1 cut(s) 198
Asp700I GAANNNNTTC 1 cut(s) 1784
AspLEI GCGC 1 cut(s) 2148
AspS9I GGNCC 8 cut(s) 610, 628, 1425, 1978, 3126, 3234, 3644, 3665
AsuC2I CCSGG 3 cut(s) 1687, 1688, 3829
AsuHPI GGTGA 8 cut(s) 332, 551, 593, 659, 1843, 2116, 3306, 3766
AsuII TTCGAA 2 cut(s) 126, 2424
AsuNHI GCTAGC 2 cut(s) 961, 2461
AvaI CYCGRG 4 cut(s) 65, 1686, 2621, 3230
AvaII GGWCC 4 cut(s) 610, 628, 3126, 3665
BaeGI GKGCMC 2 cut(s) 379, 2218
BaeI ACNNNNGTAYC 6 cut(s) 187, 220, 225, 258, 3564, 3597
BalI TGGCCA 1 cut(s) 3783
BamHI GGATCC 2 cut(s) 2158, 2365
BanI GGYRCC 1 cut(s) 3824
BanII GRGCYC 1 cut(s) 2094
BarI GAAGNNNNNNTAC 2 cut(s) 1917, 1949
BbrPI CACGTG 1 cut(s) 2168
BbsI GAAGAC 6 cut(s) 504, 1197, 1777, 1932, 2090, 2538
Bbv12I GWGCWC 2 cut(s) 2094, 3262
BceAI ACGGC 3 cut(s) 1330, 1794, 1974
BcgI CGANNNNNNTGC 8 cut(s) 2275, 2309, 2545, 2579, 2884, 2918, 3307, 3341
BciT130I CCWGG 8 cut(s) 318, 563, 1083, 1662, 2001, 2661, 2826, 3732
BciVI GTATCC 2 cut(s) 746, 2706
BclI TGATCA 1 cut(s) 2455
BcnI CCSGG 3 cut(s) 1687, 1688, 3829
BcoDI GTCTC 7 cut(s) 254, 578, 718, 969, 2168, 2747, 3081
BcuI ACTAGT 1 cut(s) 3575
BfaI CTAG 9 cut(s) 833, 962, 1277, 2025, 2324, 2462, 2760, 3576, 3723
BfmI CTRYAG 4 cut(s) 1143, 1170, 2359, 3177
BfuI GTATCC 2 cut(s) 746, 2706
BglI GCCNNNNNGGC 1 cut(s) 1986
BglII AGATCT 1 cut(s) 2242
Bme18I GGWCC 4 cut(s) 610, 628, 3126, 3665
BmeT110I CYCGRG 4 cut(s) 65, 1686, 2621, 3230
BmgBI CACGTC 1 cut(s) 2785
BmgT120I GGNCC 8 cut(s) 610, 628, 1425, 1978, 3126, 3234, 3644, 3665
BmrI ACTGGG 1 cut(s) 597
BmsI GCATC 8 cut(s) 49, 819, 856, 948, 1021, 2283, 2547, 3211
BmtI GCTAGC 2 cut(s) 965, 2465
BmuI ACTGGG 1 cut(s) 597
BoxI GACNNNNGTC 1 cut(s) 1884
BpiI GAAGAC 6 cut(s) 504, 1197, 1777, 1932, 2090, 2538
BplI GAGNNNNNCTC 4 cut(s) 698, 730, 700, 732
BpmI CTGGAG 2 cut(s) 2934, 3350
Bpu14I TTCGAA 2 cut(s) 126, 2424
BpuEI CTTGAG 5 cut(s) 502, 2711, 3132, 3181, 3658
BpuMI CCSGG 3 cut(s) 1687, 1688, 3829
BsaAI YACGTR 2 cut(s) 2168, 3413
BsaBI GATNNNNATC 1 cut(s) 1554
BsaI GGTCTC 1 cut(s) 2168
BsaWI WCCGGW 3 cut(s) 198, 1334, 3295
BsaXI ACNNNNNCTCC 4 cut(s) 720, 750, 3372, 3402
Bsc4I CCNNNNNNNGG 8 cut(s) 637, 1423, 1802, 2705, 3230, 3455, 3569, 3716
Bse118I RCCGGY 3 cut(s) 110, 198, 2687
Bse1I ACTGG 3 cut(s) 592, 1888, 3788
Bse3DI GCAATG 3 cut(s) 2042, 2191, 2469
Bse8I GATNNNNATC 1 cut(s) 1554
BseBI CCWGG 8 cut(s) 318, 563, 1083, 1662, 2001, 2661, 2826, 3732
BseJI GATNNNNATC 1 cut(s) 1554
BseLI CCNNNNNNNGG 8 cut(s) 637, 1423, 1802, 2705, 3230, 3455, 3569, 3716
BseMI GCAATG 3 cut(s) 2042, 2191, 2469
BseMII CTCAG 6 cut(s) 265, 696, 702, 731, 2055, 3556
BseNI ACTGG 3 cut(s) 592, 1888, 3788
BseRI GAGGAG 4 cut(s) 741, 2102, 2712, 3074
BseSI GKGCMC 2 cut(s) 379, 2218
BseYI CCCAGC 3 cut(s) 378, 779, 2329
BsgI GTGCAG 5 cut(s) 790, 2190, 2878, 3492, 3678
Bsh1236I CGCG 2 cut(s) 2865, 3057
BshNI GGYRCC 1 cut(s) 3824
BshTI ACCGGT 1 cut(s) 198
BsiHKAI GWGCWC 2 cut(s) 2094, 3262
BsiHKCI CYCGRG 4 cut(s) 65, 1686, 2621, 3230
BsiSI CCGG 7 cut(s) 111, 199, 1335, 1687, 2688, 3296, 3828
BslFI GGGAC 5 cut(s) 404, 596, 1682, 1779, 3538
BslI CCNNNNNNNGG 8 cut(s) 637, 1423, 1802, 2705, 3230, 3455, 3569, 3716
BsmAI GTCTC 7 cut(s) 254, 578, 718, 969, 2168, 2747, 3081
BsmBI CGTCTC 1 cut(s) 3081
BsmFI GGGAC 5 cut(s) 404, 596, 1682, 1779, 3538
BsmI GAATGC 4 cut(s) 1324, 2082, 2129, 2653
Bso31I GGTCTC 1 cut(s) 2168
BsoBI CYCGRG 4 cut(s) 65, 1686, 2621, 3230
Bsp119I TTCGAA 2 cut(s) 126, 2424
Bsp1286I GDGCHC 4 cut(s) 379, 2094, 2218, 3262
Bsp19I CCATGG 1 cut(s) 3520
BspACI CCGC 8 cut(s) 70, 382, 594, 1443, 2865, 3363, 3588, 3671
BspCNI CTCAG 6 cut(s) 264, 697, 701, 730, 2056, 3555
BspFNI CGCG 2 cut(s) 2865, 3057
BspHI TCATGA 1 cut(s) 931
BspMAI CTGCAG 1 cut(s) 1147
BspOI GCTAGC 2 cut(s) 965, 2465
BspPI GGATC 7 cut(s) 948, 1904, 2153, 2166, 2360, 2373, 3293
BspT104I TTCGAA 2 cut(s) 126, 2424
BspT107I GGYRCC 1 cut(s) 3824
BspTNI GGTCTC 1 cut(s) 2168
BsrBI CCGCTC 1 cut(s) 70
BsrDI GCAATG 3 cut(s) 2042, 2191, 2469
BsrFI RCCGGY 3 cut(s) 110, 198, 2687
BsrI ACTGG 3 cut(s) 592, 1888, 3788
BssAI RCCGGY 3 cut(s) 110, 198, 2687
BssNAI GTATAC 1 cut(s) 2666
BssT1I CCWWGG 3 cut(s) 1428, 3206, 3520
Bst1107I GTATAC 1 cut(s) 2666
Bst2UI CCWGG 8 cut(s) 318, 563, 1083, 1662, 2001, 2661, 2826, 3732
Bst6I CTCTTC 3 cut(s) 170, 380, 1938
BstAPI GCANNNNNTGC 1 cut(s) 2471
BstBAI YACGTR 2 cut(s) 2168, 3413
BstBI TTCGAA 2 cut(s) 126, 2424
BstC8I GCNNGC 7 cut(s) 407, 963, 1221, 1391, 1987, 1997, 2463
BstDSI CCRYGG 1 cut(s) 3520
BstEII GGTNACC 1 cut(s) 3726
BstFNI CGCG 2 cut(s) 2865, 3057
BstHHI GCGC 1 cut(s) 2148
BstMAI GTCTC 7 cut(s) 254, 578, 718, 969, 2168, 2747, 3081
BstMWI GCNNNNNNNGC 8 cut(s) 1844, 1986, 2414, 2471, 2584, 2605, 3233, 3596
BstNI CCWGG 8 cut(s) 318, 563, 1083, 1662, 2001, 2661, 2826, 3732
BstNSI RCATGY 7 cut(s) 637, 657, 982, 1393, 2349, 2566, 3258
BstPAI GACNNNNGTC 1 cut(s) 1884
BstPI GGTNACC 1 cut(s) 3726
BstSFI CTRYAG 4 cut(s) 1143, 1170, 2359, 3177
BstSLI GKGCMC 2 cut(s) 379, 2218
BstUI CGCG 2 cut(s) 2865, 3057
BstV2I GAAGAC 6 cut(s) 504, 1197, 1777, 1932, 2090, 2538
BstX2I RGATCY 3 cut(s) 2158, 2242, 2365
BstYI RGATCY 3 cut(s) 2158, 2242, 2365
BstZ17I GTATAC 1 cut(s) 2666
BsuI GTATCC 2 cut(s) 746, 2706
BtgI CCRYGG 1 cut(s) 3520
BtrI CACGTC 1 cut(s) 2785
BtsI GCAGTG 1 cut(s) 3606
BtsIMutI CAGTG 7 cut(s) 234, 1167, 1881, 2216, 2279, 2895, 3606
Cac8I GCNNGC 7 cut(s) 407, 963, 1221, 1391, 1987, 1997, 2463
CaiI CAGNNNCTG 2 cut(s) 704, 2195
CciI TCATGA 1 cut(s) 931
CfoI GCGC 1 cut(s) 2148
Cfr10I RCCGGY 3 cut(s) 110, 198, 2687
Cfr13I GGNCC 8 cut(s) 610, 628, 1425, 1978, 3126, 3234, 3644, 3665
Cfr9I CCCGGG 1 cut(s) 1686
CsiI ACCWGGT 2 cut(s) 316, 561
Csp6I GTAC 9 cut(s) 242, 305, 669, 1124, 2995, 3343, 3573, 3581, 3720
CspAI ACCGGT 1 cut(s) 198
CspCI CAANNNNNGTGG 6 cut(s) 423, 458, 682, 717, 3622, 3657
CviQI GTAC 9 cut(s) 242, 305, 669, 1124, 2995, 3343, 3573, 3581, 3720
DraIII CACNNNGTG 1 cut(s) 320
EaeI YGGCCR 2 cut(s) 2685, 3781
Eam1104I CTCTTC 3 cut(s) 170, 380, 1938
EarI CTCTTC 3 cut(s) 170, 380, 1938
Ecl136II GAGCTC 1 cut(s) 2092
Eco130I CCWWGG 3 cut(s) 1428, 3206, 3520
Eco147I AGGCCT 1 cut(s) 3559
Eco24I GRGCYC 1 cut(s) 2094
Eco31I GGTCTC 1 cut(s) 2168
Eco47I GGWCC 4 cut(s) 610, 628, 3126, 3665
Eco53kI GAGCTC 1 cut(s) 2092
Eco57I CTGAAG 5 cut(s) 681, 1209, 1314, 1919, 2084
Eco72I CACGTG 1 cut(s) 2168
Eco88I CYCGRG 4 cut(s) 65, 1686, 2621, 3230
Eco91I GGTNACC 1 cut(s) 3726
EcoICRI GAGCTC 1 cut(s) 2092
EcoO109I RGGNCCY 2 cut(s) 610, 3234
EcoO65I GGTNACC 1 cut(s) 3726
EcoRI GAATTC 1 cut(s) 2138
EcoRII CCWGG 8 cut(s) 316, 561, 1081, 1660, 1999, 2659, 2824, 3730
EcoT14I CCWWGG 3 cut(s) 1428, 3206, 3520
EcoT38I GRGCYC 1 cut(s) 2094
ErhI CCWWGG 3 cut(s) 1428, 3206, 3520
Esp3I CGTCTC 1 cut(s) 3081
FalI AAGNNNNNCTT 4 cut(s) 1203, 1235, 1769, 1801
FaqI GGGAC 5 cut(s) 404, 596, 1682, 1779, 3538
FauI CCCGC 1 cut(s) 2858
FauNDI CATATG 1 cut(s) 1381
FbaI TGATCA 1 cut(s) 2455
FblI GTMKAC 1 cut(s) 2665
FriOI GRGCYC 1 cut(s) 2094
FspBI CTAG 9 cut(s) 833, 962, 1277, 2025, 2324, 2462, 2760, 3576, 3723
FspI TGCGCA 1 cut(s) 2147
GlaI GCGC 1 cut(s) 2147
GsaI CCCAGC 3 cut(s) 382, 783, 2333
GsuI CTGGAG 2 cut(s) 2934, 3350
HapII CCGG 7 cut(s) 111, 199, 1335, 1687, 2688, 3296, 3828
HhaI GCGC 1 cut(s) 2148
Hin6I GCGC 1 cut(s) 2146
HinP1I GCGC 1 cut(s) 2146
HincII GTYRAC 1 cut(s) 3798
HindII GTYRAC 1 cut(s) 3798
HindIII AAGCTT 3 cut(s) 509, 1217, 2037
HpaII CCGG 7 cut(s) 111, 199, 1335, 1687, 2688, 3296, 3828
HphI GGTGA 8 cut(s) 332, 551, 593, 659, 1843, 2116, 3306, 3766
Hpy99I CGWCG 1 cut(s) 684
HpyAV CCTTC 8 cut(s) 266, 1283, 1289, 1667, 1774, 2485, 3657, 3710
HpyCH4IV ACGT 7 cut(s) 398, 682, 1792, 2167, 2784, 2833, 3412
HpyF10VI GCNNNNNNNGC 8 cut(s) 1844, 1986, 2414, 2471, 2584, 2605, 3233, 3596
HpySE526I ACGT 7 cut(s) 398, 682, 1792, 2167, 2784, 2833, 3412
HspAI GCGC 1 cut(s) 2146
Ksp22I TGATCA 1 cut(s) 2455
LmnI GCTCC 7 cut(s) 1640, 2059, 2089, 2592, 2596, 2953, 3596
LweI GCATC 8 cut(s) 49, 819, 856, 948, 1021, 2283, 2547, 3211
MabI ACCWGGT 2 cut(s) 316, 561
MaeI CTAG 9 cut(s) 833, 962, 1277, 2025, 2324, 2462, 2760, 3576, 3723
MaeII ACGT 7 cut(s) 398, 682, 1792, 2167, 2784, 2833, 3412
MbiI CCGCTC 1 cut(s) 70
MfeI CAATTG 3 cut(s) 795, 2082, 2777
MflI RGATCY 3 cut(s) 2158, 2242, 2365
MhlI GDGCHC 4 cut(s) 379, 2094, 2218, 3262
MlsI TGGCCA 1 cut(s) 3783
MluNI TGGCCA 1 cut(s) 3783
MlyI GAGTC 4 cut(s) 720, 2332, 3320, 3343
MmeI TCCRAC 5 cut(s) 718, 747, 1868, 3415, 3643
Mox20I TGGCCA 1 cut(s) 3783
MroXI GAANNNNTTC 1 cut(s) 1784
MscI TGGCCA 1 cut(s) 3783
MslI CAYNNNNRTG 1 cut(s) 917
Msp20I TGGCCA 1 cut(s) 3783
MspA1I CMGCKG 2 cut(s) 382, 3363
MspI CCGG 7 cut(s) 111, 199, 1335, 1687, 2688, 3296, 3828
MunI CAATTG 3 cut(s) 795, 2082, 2777
Mva1269I GAATGC 4 cut(s) 1324, 2082, 2129, 2653
MvaI CCWGG 8 cut(s) 318, 563, 1083, 1662, 2001, 2661, 2826, 3732
MvnI CGCG 2 cut(s) 2865, 3057
MwoI GCNNNNNNNGC 8 cut(s) 1844, 1986, 2414, 2471, 2584, 2605, 3233, 3596
NciI CCSGG 3 cut(s) 1687, 1688, 3829
NcoI CCATGG 1 cut(s) 3520
NdeI CATATG 1 cut(s) 1381
NheI GCTAGC 2 cut(s) 961, 2461
NmeAIII GCCGAG 1 cut(s) 2006
NmuCI GTSAC 4 cut(s) 293, 2220, 3312, 3653
NsbI TGCGCA 1 cut(s) 2147
NspI RCATGY 7 cut(s) 637, 657, 982, 1393, 2349, 2566, 3258
NspV TTCGAA 2 cut(s) 126, 2424
PaeI GCATGC 1 cut(s) 1393
PaeR7I CTCGAG 1 cut(s) 65
PagI TCATGA 1 cut(s) 931
PceI AGGCCT 1 cut(s) 3559
PciI ACATGT 1 cut(s) 633
PctI GAATGC 4 cut(s) 1324, 2082, 2129, 2653
PdmI GAANNNNTTC 1 cut(s) 1784
PfeI GAWTC 7 cut(s) 446, 554, 1628, 1705, 1721, 2004, 3302
PinAI ACCGGT 1 cut(s) 198
PleI GAGTC 4 cut(s) 719, 2332, 3319, 3342
PmaCI CACGTG 1 cut(s) 2168
PmlI CACGTG 1 cut(s) 2168
PpsI GAGTC 4 cut(s) 719, 2332, 3319, 3342
Ppu21I YACGTR 2 cut(s) 2168, 3413
PpuMI RGGWCCY 1 cut(s) 610
PscI ACATGT 1 cut(s) 633
PshAI GACNNNNGTC 1 cut(s) 1884
PshBI ATTAAT 3 cut(s) 1013, 1239, 3738
Psp124BI GAGCTC 1 cut(s) 2094
Psp5II RGGWCCY 1 cut(s) 610
Psp6I CCWGG 8 cut(s) 316, 561, 1081, 1660, 1999, 2659, 2824, 3730
PspCI CACGTG 1 cut(s) 2168
PspEI GGTNACC 1 cut(s) 3726
PspFI CCCAGC 3 cut(s) 378, 779, 2329
PspGI CCWGG 8 cut(s) 316, 561, 1081, 1660, 1999, 2659, 2824, 3730
PspPI GGNCC 8 cut(s) 610, 628, 1425, 1978, 3126, 3234, 3644, 3665
PspPPI RGGWCCY 1 cut(s) 610
PspXI VCTCGAGB 1 cut(s) 65
PsrI GAACNNNNNNTAC 2 cut(s) 1527, 1559
PstI CTGCAG 1 cut(s) 1147
PstNI CAGNNNCTG 2 cut(s) 704, 2195
PsuI RGATCY 3 cut(s) 2158, 2242, 2365
RsaI GTAC 9 cut(s) 243, 306, 670, 1125, 2996, 3344, 3574, 3582, 3721
RsaNI GTAC 9 cut(s) 242, 305, 669, 1124, 2995, 3343, 3573, 3581, 3720
RseI CAYNNNNRTG 1 cut(s) 917
SacI GAGCTC 1 cut(s) 2094
Sau96I GGNCC 8 cut(s) 610, 628, 1425, 1978, 3126, 3234, 3644, 3665
SchI GAGTC 4 cut(s) 720, 2332, 3320, 3343
SduI GDGCHC 4 cut(s) 379, 2094, 2218, 3262
SexAI ACCWGGT 2 cut(s) 316, 561
SfaNI GCATC 8 cut(s) 49, 819, 856, 948, 1021, 2283, 2547, 3211
SfcI CTRYAG 4 cut(s) 1143, 1170, 2359, 3177
SfiI GGCCNNNNNGGCC 1 cut(s) 1986
Sfr274I CTCGAG 1 cut(s) 65
SfuI TTCGAA 2 cut(s) 126, 2424
SgrAI CRCCGGYG 1 cut(s) 198
SinI GGWCC 4 cut(s) 610, 628, 3126, 3665
SlaI CTCGAG 1 cut(s) 65
SmaI CCCGGG 1 cut(s) 1688
SmiMI CAYNNNNRTG 1 cut(s) 917
SmlI CTYRAG 6 cut(s) 65, 481, 2726, 3147, 3160, 3637
SmoI CTYRAG 6 cut(s) 65, 481, 2726, 3147, 3160, 3637
SpeI ACTAGT 1 cut(s) 3575
SphI GCATGC 1 cut(s) 1393
SseBI AGGCCT 1 cut(s) 3559
SsiI CCGC 8 cut(s) 70, 382, 594, 1443, 2865, 3363, 3588, 3671
SspI AATATT 3 cut(s) 1052, 1565, 1582
SspMI CTAG 9 cut(s) 833, 962, 1277, 2025, 2324, 2462, 2760, 3576, 3723
SstI GAGCTC 1 cut(s) 2094
StuI AGGCCT 1 cut(s) 3559
StyI CCWWGG 3 cut(s) 1428, 3206, 3520
TaiI ACGT 7 cut(s) 401, 685, 1795, 2170, 2787, 2836, 3415
TatI WGTACW 4 cut(s) 668, 1123, 2994, 3580
TauI GCSGC 1 cut(s) 3590
TfiI GAWTC 7 cut(s) 446, 554, 1628, 1705, 1721, 2004, 3302
TscAI CASTG 7 cut(s) 241, 1174, 1888, 2223, 2286, 2902, 3606
TseFI GTSAC 4 cut(s) 293, 2220, 3312, 3653
Tsp45I GTSAC 4 cut(s) 293, 2220, 3312, 3653
TspGWI ACGGA 3 cut(s) 1064, 2660, 2902
TspMI CCCGGG 1 cut(s) 1686
TspRI CASTG 7 cut(s) 241, 1174, 1888, 2223, 2286, 2902, 3606
VpaK11BI GGWCC 4 cut(s) 610, 628, 3126, 3665
VspI ATTAAT 3 cut(s) 1013, 1239, 3738
XapI RAATTY 6 cut(s) 816, 927, 1510, 1617, 2138, 2420
XbaI TCTAGA 3 cut(s) 1276, 2024, 2323
XceI RCATGY 7 cut(s) 637, 657, 982, 1393, 2349, 2566, 3258
XhoI CTCGAG 1 cut(s) 65
XmaI CCCGGG 1 cut(s) 1686
XmiI GTMKAC 1 cut(s) 2665
XmnI GAANNNNTTC 1 cut(s) 1784
XspI CTAG 9 cut(s) 833, 962, 1277, 2025, 2324, 2462, 2760, 3576, 3723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.