Rh2CG480900

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0015155
Physical Location & Seq
Reverse (-)
64558331 .. 64564776
6446 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG480900.1

Sequence Viewer

Length: 3663 bp
ATGGTGAGGACGAAGAAAGGAGGACGACCGCCCACTCTGATTTCAGAAGAGGCAAGGATCCGAATACGTGACATACTCCAGCAGTTCCTGGAGTCCAATGAAGAAGTTTACACATTTGGGACAGACCTGACGGTTAATGAACGGGCTTACGTCCACGAACAGTGCAGCAAGATGGATATGAAATCCAAGAGCTCCGGGTCGGGTAAAACGCGCAAGGTTTCGGTTTACAGGCTTTTTAGGATGGAGGCAATCAGCAATGGTAAGGAAAGAATCCCTCAGGTGACGTTTTCCGGAGACACAAGAGAAGTTTTGGGGGATTTGTTTACGTGGTACCCACCCGGAGATGGAGAGCAACCCAAAGAAAGGATCCGTGAAGATAGTGACAAGTTTCAGAAACTCCACCGCAAGAAAGACCCGCTGTTCTGCAGACCTTCGATGAGCCAGAACGAAATTGCAAAGAAAGTGGAAATGCAGGCTACTAAAATGAGAAAAGCCAAGATTACTAAAGAGAGGTCTAAGCTACCCATGGCACCCTACAGGGATGTGGTAACGTCAGCAGTTGAGTCTCACCAGGTGCCACAATATCTTTTGGACCATAAGTGGGGTAAGGGTGAGGCTTGTAAAATAGTGTGCACTCAACCCAGGCGTATCTCAGCCATATCAGTTGCTGAGAGAATCTCTTATGAAAGAGGACAGAATGTTGGGGAGGATATTGGATACAAGATACGGTTGGAAAGCAAAGGTGGGAGGCACTCATCTATGGTGTTCTGCACAAATGGGATTCTACTGAGGATTCTAATTTCCAGAGGCGGTGGTAGGTCAAAAACAGATGCATCGAATAAAGAAACCAAACAACAAGATCTCTCTGACATAACTCACATTATTGTGGATGAAATTCATGAAAGGGATCGCCACTCTGATTTCATTCTGGCTATTCTCAGAGACATGCTTCCTTCACATCCTCATCTGCATCTGATATTGATGAGTGCGACTGTTGATGTGGAACGGTTTTCACAATATTTTGGTGGCTGCCCAATTATCCGTGTCCCAGGGTTCACTTATCCTGTCAAATCTTTCTATTTGGAGGATGTATTGTCTATCTTGAGATCTGTACAAAATAATCACTTAGATAACCCTGCATTAGGTGCCTCAACTGCAGACCGTGACTTATCAGAGGAAGATAAGATTGCTTTAGATGAAGCAATTAGTTTGGCTTGGTCAGATGATGAATTTGATCCACTTCTAGAATTGGTTTCTACTGAAGGAACCCGTAAAATCTTTAATTACCAGCATTCTGTAACTGGACTTACACCATTAATGGTATTTGCTGGAAGGGGTAGAGTAGGAGATGTTTGCATGCTGCTATCATTTGGTGCAGACTGCCAATTACGGGCCAAGGATGGAACCACTGCATTAGAGTTGGCTGAGCAGGTAAACCAATTAGAATCTGCTCAGATGTTGAAAGAGCATATGGAAAGTGCTCGTTCTAACTCTACGGAAGAACAAAATTTGCTCAATAAATATCTCCAATATTTTAATCCTGAGAATATTGATTTCATTCTTATCGAGCAATTGTTAGGGAAAATTTTTAGTGATTCGAAAGATGGAGCTATTCTTGTTTTTCTTCCAGGGTGGGATGATATAAATAAAATGAGGGACAGATTACTCACGAACCCATATTTCAGAGATCCTTCTAAGTGTGTGATTATATCTCTACATTCAATGGTTCCAGCTGCTGAACAGAAGAAGGTCTTCATTCGTCCACCTAGTGGTTGCCGAAAAATTGTTCTTTCAACCAATATTGCTGAAACTGCAGTCACTATTGATGATGTTGTTTATGTTATAAACACGGGTCGGATGAAAGAAAAAAATTATGATCCTTTTAGTAATGTTTCAACTCTTCAATCTTCTTGGGTGTCAAAAGCGAATGCCAAGCAGCGACAGGGGCGTGCGGGCCGTTGTCAGCCTGGAATCTGTTATCATCTTTACTCTAAACTTCGAGCAGCTGCATTGTCAGATTTTCAGGTGCCTGAGATTAGGAGAATGCCAATCGAGGAACTTTGCCTTCAGGTGAAGTTGCTTGATCCAGATTGCAAGATAGACGAGTTCCTAAACAAGACGCTGGATCCTCCTCATTCTGAGACCATACACAATGCGATTTCTGTTCTTCGAGATATCGGGGCTTTGTCGGTGGACGATAAACTTACAGAACTTGGGGAAAAGCTAGGTTCTTTACCTGTTCATCCATTGACAAGCAAGATGCTATTCTTTGCCACACTGATGAACTGTCTAGACCCAGCTTTAACTCTGGCCTGTGCTGCCGACTACAAGGATCCATTCTCTCTTCCTATGATGCCTGAGGAAAAGGAGAAAGCTGCTGCTGCTAAGGCTGAACTGTGTTCATTGTATGGTGGGAACAGTGATCAGCTAGCAGTCATAGCAGCATTTGACTGCTGGAAGATTGCAAAAGAACAGGGTAAAGAGAATCCGTTCTGTGCTAAATACTTTGTCTCTGCAAGTACCATGCAGATGCTATCTGGTATGCGAAAGCAACTCCAAACAGAGCTAATTCGGCTGGGGTTTATTCCTGAGGATGTGTCTAGATGTAGCTTGAATGCACGCAATCCAGGTATAATCCACGCTGTCCTTGTGGCTGGTCTGTATCCCATGTTGGGAAGATTGCTTTCTTCTACTAAGAGTGGGAAAAAAAAAGGATCCTTTGTAGAAATTCCTGATGGTAGTAAAGTTCGTTTGCACCCTCGATCTGCTAATTTTAAAATAGCGTTCAAGGCACGTGGTGGCTCTCCTTTGGTTGTATATGATGAGATAACCCGTGGGGATGGGGGTTCGAATATACGAAACTGTACTGTTGTCGGGCCACTTCCATTAATATTGCTGTCGACAGAAATAGCTGTTGCTCCGGCCAATGATGATGAGGGAAGTGATGTCGATGATAATGAGTATGAAAGTGATGAAGATGCAATGAAGATAGATTCTAAGTCGGGTGAACAGGCCGAAGAGAAGATTATGTCATCACCTGATAAATCTGTCATGGTTATAGTGGATGGGTGGCTTTGTTTTGAGTCGAAAGCTCTTGACGTTGCTCAGATCTATTGCTTGAGGGAACGCCTGTCTGCAGCAATACTATTTACGGTTACTCATCCAAAGGAAGTTTTACCTCCTGATCTTGGGGCATCTATTTATGCTATAGCCTGCGTTCTATCTTATGATGGTCTATCTGGGATTCCATTGCCAAAGCCTATGGATTTGCCGAATTCAGATTTAGAAGAACTTGAGATCGGTAAAGATGGAAAGGCACAGTTTAAAGTGACAGGAAATAAAGCAGCAAAATCAAAGGAGCCATTACGTCAGAGAGTAGTTTGTCAGAGGTCTCTATCGCGAGCTACTGATTTACCTGCCTTAAATGGATTTGCAAAGTTGTGTGACGACCCATCAATTCAGAGTGTTCCCCCACCGGCTTTCGCGAACGCTACTGATTTACCTGCCTTAATAAATGGAGTTGCAAAGTTGAGTGGCGACCGATCACTTCACAGTGTTCCCCCACCAGCTTTTGCGAACACAATCCCGCCGCCCACTTCACAAGTTCCAGCAGGTCCTGTCGCAGGAAACAAGCGGAAGAAGCGCCGGAATCAGGCTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0006139 GO:0006259 GO:0006396 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007276 GO:0007281 GO:0007283 GO:0007286 GO:0007292 GO:0007346 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0009994 GO:0010033 GO:0010467 GO:0012505 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019899 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0030154 GO:0032392 GO:0032501 GO:0032502 GO:0032504 GO:0032508 GO:0032991 GO:0034097 GO:0034458 GO:0034612 GO:0034641 GO:0035770 GO:0035821 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043900 GO:0043902 GO:0043903 GO:0044237 GO:0044238 GO:0044260 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044788 GO:0044794 GO:0044827 GO:0044829 GO:0045069 GO:0045070 GO:0045786 GO:0045787 GO:0045930 GO:0046483 GO:0048232 GO:0048468 GO:0048477 GO:0048515 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048524 GO:0048599 GO:0048609 GO:0048856 GO:0048869 GO:0050789 GO:0050792 GO:0050794 GO:0050896 GO:0051276 GO:0051445 GO:0051446 GO:0051702 GO:0051704 GO:0051726 GO:0051728 GO:0051729 GO:0051817 GO:0051851 GO:0060184 GO:0065007 GO:0070035 GO:0070063 GO:0070555 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140097 GO:0140098 GO:1901360 GO:1901363 GO:1903900 GO:1903902 GO:1990247 GO:1990904 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind R3H

Protein Analysis

1220

Amino Acids

135.24

Weight (kDa)

7.47

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
R3H PF01424 20 - 77 2.2e-08 R3H domain
Helicase_C PF00271 525 - 654 8.9e-11 Helicase conserved C-terminal domain
HA2_C PF21010 749 - 810 1.6e-08 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 879 - 969 8.7e-11 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015155)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G30800 AT2G30800
fragaria_vesca FvH4_6g38870
malus_domestica MD09G1146500.v1.1
prunus_persica Prupe.3G179000_v2.0.a1
pyrus_communis pycom09g06680
rosa_chinensis RchiOBHm_Chr2g0152461
rosa_laevigata RLG00000020635
rosa_multiflora Rmu_sc0005366.1_g000012
rosa_roxburghii Rroxscaffold_2G00095700
rosa_rugosa Rorug02G0432900
rosa_samantha Rh2AG494700 Rh2BG506900 Rh2CG480900 Rh2DG518100
rosa_wichuraiana Rw2G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1844
Acc36I ACCTGC 4 cut(s) 1420, 3424, 3511, 3602
Acc65I GGTACC 1 cut(s) 330
AccB1I GGYRCC 5 cut(s) 330, 529, 574, 1145, 2026
AccB7I CCANNNNNTGG 1 cut(s) 1769
AccI GTMKAC 1 cut(s) 2900
AccII CGCG 3 cut(s) 211, 3400, 3485
AccIII TCCGGA 1 cut(s) 290
AciI CCGC 8 cut(s) 29, 403, 416, 810, 1952, 3587, 3590, 3634
AcoI YGGCCR 1 cut(s) 2922
AcsI RAATTY 6 cut(s) 894, 1229, 1507, 1584, 2727, 3274
AcuI CTGAAG 2 cut(s) 1281, 2051
AcvI CACGTG 1 cut(s) 2795
AdeI CACNNNGTG 3 cut(s) 574, 1769, 2798
AfaI GTAC 4 cut(s) 332, 1113, 2521, 2866
AgsI TTSAA 7 cut(s) 1462, 1722, 1794, 1896, 1904, 2614, 2788
AjnI CCWGG 7 cut(s) 87, 570, 641, 1048, 1627, 1966, 2626
Alw21I GWGCWC 3 cut(s) 194, 635, 1483
Alw26I GTCTC 6 cut(s) 288, 570, 936, 2135, 2515, 3396
Alw44I GTGCAC 1 cut(s) 631
AlwNI CAGNNNCTG 4 cut(s) 88, 668, 1736, 3617
Aor13HI TCCGGA 1 cut(s) 290
AoxI GGCC 6 cut(s) 1392, 1954, 2310, 2876, 2922, 3012
ApaLI GTGCAC 1 cut(s) 631
ApoI RAATTY 6 cut(s) 894, 1229, 1507, 1584, 2727, 3274
AseI ATTAAT 2 cut(s) 1316, 2888
Asp700I GAANNNNTTC 2 cut(s) 1751, 2489
Asp718I GGTACC 1 cut(s) 330
AspLEI GCGC 2 cut(s) 213, 3645
AspS9I GGNCC 5 cut(s) 592, 1392, 1954, 2876, 3614
AsuC2I CCSGG 2 cut(s) 196, 339
AsuHPI GGTGA 7 cut(s) 16, 292, 560, 623, 2083, 3017, 3027
AsuII TTCGAA 2 cut(s) 1598, 2849
AsuNHI GCTAGC 1 cut(s) 2428
AvaII GGWCC 2 cut(s) 592, 3614
AxyI CCTNAGG 3 cut(s) 276, 2358, 2589
BaeGI GKGCMC 1 cut(s) 635
BamHI GGATCC 5 cut(s) 57, 366, 2125, 2332, 2714
BanI GGYRCC 5 cut(s) 330, 529, 574, 1145, 2026
BanII GRGCYC 1 cut(s) 194
BbrPI CACGTG 1 cut(s) 2795
BbsI GAAGAC 1 cut(s) 1744
Bbv12I GWGCWC 3 cut(s) 194, 635, 1483
BceAI ACGGC 1 cut(s) 1941
BciT130I CCWGG 7 cut(s) 89, 572, 643, 1050, 1629, 1968, 2628
BciVI GTATCC 2 cut(s) 710, 2673
BclI TGATCA 1 cut(s) 2422
BcnI CCSGG 2 cut(s) 196, 339
BcoDI GTCTC 6 cut(s) 288, 570, 936, 2135, 2515, 3396
BfaI CTAG 6 cut(s) 1244, 1767, 2225, 2291, 2429, 2601
BfmI CTRYAG 6 cut(s) 424, 535, 1155, 1812, 3135, 3207
BfoI RGCGCY 1 cut(s) 3646
BfuAI ACCTGC 4 cut(s) 1420, 3424, 3511, 3602
BfuI GTATCC 2 cut(s) 710, 2673
BglII AGATCT 3 cut(s) 859, 1106, 3108
BlpI GCTNAGC 1 cut(s) 1425
Bme1390I CCNGG 9 cut(s) 89, 196, 339, 572, 643, 1050, 1629, 1968, 2628
Bme18I GGWCC 2 cut(s) 592, 3614
BmgT120I GGNCC 5 cut(s) 592, 1392, 1954, 2876, 3614
BmrFI CCNGG 9 cut(s) 89, 196, 339, 572, 643, 1050, 1629, 1968, 2628
BmsI GCATC 8 cut(s) 820, 842, 979, 2250, 2343, 2520, 2968, 3203
BmtI GCTAGC 1 cut(s) 2432
BpiI GAAGAC 1 cut(s) 1744
BplI GAGNNNNNCTC 2 cut(s) 662, 694
BpmI CTGGAG 2 cut(s) 62, 110
Bpu10I CCTNAGC 1 cut(s) 2385
Bpu1102I GCTNAGC 1 cut(s) 1425
Bpu14I TTCGAA 2 cut(s) 1598, 2849
BpuEI CTTGAG 3 cut(s) 1123, 3139, 3314
BpuMI CCSGG 2 cut(s) 196, 339
BsaAI YACGTR 3 cut(s) 68, 327, 2795
BsaI GGTCTC 2 cut(s) 2135, 3396
BsaJI CCNNGG 7 cut(s) 525, 641, 1048, 1049, 1395, 1628, 2833
BsaWI WCCGGW 1 cut(s) 290
Bse118I RCCGGY 1 cut(s) 3475
Bse1I ACTGG 1 cut(s) 1306
Bse21I CCTNAGG 3 cut(s) 276, 2358, 2589
Bse3DI GCAATG 3 cut(s) 262, 2988, 3248
BseAI TCCGGA 1 cut(s) 290
BseBI CCWGG 7 cut(s) 89, 572, 643, 1050, 1629, 1968, 2628
BseDI CCNNGG 7 cut(s) 525, 641, 1048, 1049, 1395, 1628, 2833
BseMI GCAATG 3 cut(s) 262, 2988, 3248
BseNI ACTGG 1 cut(s) 1306
BseRI GAGGAG 1 cut(s) 2121
BseSI GKGCMC 1 cut(s) 635
BseYI CCCAGC 2 cut(s) 2296, 2575
BsgI GTGCAG 3 cut(s) 184, 754, 1395
Bsh1236I CGCG 3 cut(s) 211, 3400, 3485
Bsh1285I CGRYCG 2 cut(s) 29, 3541
BshFI GGCC 6 cut(s) 1394, 1956, 2312, 2878, 2924, 3014
BshNI GGYRCC 5 cut(s) 330, 529, 574, 1145, 2026
BsiEI CGRYCG 2 cut(s) 29, 3541
BsiHKAI GWGCWC 3 cut(s) 194, 635, 1483
BsiSI CCGG 6 cut(s) 195, 291, 339, 2921, 3476, 3646
BslFI GGGAC 3 cut(s) 133, 1031, 1670
BsmAI GTCTC 6 cut(s) 288, 570, 936, 2135, 2515, 3396
BsmFI GGGAC 3 cut(s) 133, 1031, 1670
BsmI GAATGC 4 cut(s) 1291, 1933, 2049, 2620
BsnI GGCC 6 cut(s) 1394, 1956, 2312, 2878, 2924, 3014
Bso31I GGTCTC 2 cut(s) 2135, 3396
Bsp119I TTCGAA 2 cut(s) 1598, 2849
Bsp1286I GDGCHC 3 cut(s) 194, 635, 1483
Bsp13I TCCGGA 1 cut(s) 290
Bsp1407I TGTACA 1 cut(s) 1111
Bsp1720I GCTNAGC 1 cut(s) 1425
Bsp19I CCATGG 1 cut(s) 525
Bsp68I TCGCGA 2 cut(s) 3400, 3485
BspACI CCGC 8 cut(s) 29, 403, 416, 810, 1952, 3587, 3590, 3634
BspANI GGCC 6 cut(s) 1394, 1956, 2312, 2878, 2924, 3014
BspEI TCCGGA 1 cut(s) 290
BspFNI CGCG 3 cut(s) 211, 3400, 3485
BspHI TCATGA 1 cut(s) 898
BspMAI CTGCAG 4 cut(s) 428, 1159, 1816, 3139
BspMI ACCTGC 4 cut(s) 1420, 3424, 3511, 3602
BspOI GCTAGC 1 cut(s) 2432
BspT104I TTCGAA 2 cut(s) 1598, 2849
BspT107I GGYRCC 5 cut(s) 330, 529, 574, 1145, 2026
BspTNI GGTCTC 2 cut(s) 2135, 3396
BsrDI GCAATG 3 cut(s) 262, 2988, 3248
BsrFI RCCGGY 1 cut(s) 3475
BsrGI TGTACA 1 cut(s) 1111
BsrI ACTGG 1 cut(s) 1306
BssAI RCCGGY 1 cut(s) 3475
BssECI CCNNGG 7 cut(s) 525, 641, 1048, 1049, 1395, 1628, 2833
BssT1I CCWWGG 2 cut(s) 525, 1395
Bst2UI CCWGG 7 cut(s) 89, 572, 643, 1050, 1629, 1968, 2628
Bst6I CTCTTC 4 cut(s) 42, 1905, 2349, 3012
BstAPI GCANNNNNTGC 1 cut(s) 1145
BstAUI TGTACA 1 cut(s) 1111
BstBAI YACGTR 3 cut(s) 68, 327, 2795
BstBI TTCGAA 2 cut(s) 1598, 2849
BstC8I GCNNGC 8 cut(s) 474, 1358, 1950, 1954, 2430, 2620, 3214, 3402
BstDSI CCRYGG 2 cut(s) 525, 2833
BstENI CCTNNNNNAGG 2 cut(s) 1140, 3621
BstFNI CGCG 3 cut(s) 211, 3400, 3485
BstH2I RGCGCY 1 cut(s) 3646
BstHHI GCGC 2 cut(s) 213, 3645
BstMAI GTCTC 6 cut(s) 288, 570, 936, 2135, 2515, 3396
BstMCI CGRYCG 2 cut(s) 29, 3541
BstNI CCWGG 7 cut(s) 89, 572, 643, 1050, 1629, 1968, 2628
BstNSI RCATGY 2 cut(s) 949, 1360
BstSCI CCNGG 9 cut(s) 87, 194, 337, 570, 641, 1048, 1627, 1966, 2626
BstSFI CTRYAG 6 cut(s) 424, 535, 1155, 1812, 3135, 3207
BstSLI GKGCMC 1 cut(s) 635
BstUI CGCG 3 cut(s) 211, 3400, 3485
BstV2I GAAGAC 1 cut(s) 1744
BstX2I RGATCY 9 cut(s) 57, 366, 859, 1106, 1687, 2125, 2332, 2714, 3108
BstYI RGATCY 9 cut(s) 57, 366, 859, 1106, 1687, 2125, 2332, 2714, 3108
Bsu36I CCTNAGG 3 cut(s) 276, 2358, 2589
BsuI GTATCC 2 cut(s) 710, 2673
BsuRI GGCC 6 cut(s) 1394, 1956, 2312, 2878, 2924, 3014
BtgI CCRYGG 2 cut(s) 525, 2833
BtsI GCAGTG 1 cut(s) 1407
BtsIMutI CAGTG 5 cut(s) 167, 1407, 2276, 2425, 3559
BtuMI TCGCGA 2 cut(s) 3400, 3485
BveI ACCTGC 4 cut(s) 1420, 3424, 3511, 3602
Cac8I GCNNGC 8 cut(s) 474, 1358, 1950, 1954, 2430, 2620, 3214, 3402
CaiI CAGNNNCTG 4 cut(s) 88, 668, 1736, 3617
CciI TCATGA 1 cut(s) 898
CfoI GCGC 2 cut(s) 213, 3645
Cfr10I RCCGGY 1 cut(s) 3475
Cfr13I GGNCC 5 cut(s) 592, 1392, 1954, 2876, 3614
CseI GACGC 1 cut(s) 2128
CsiI ACCWGGT 1 cut(s) 570
Csp6I GTAC 4 cut(s) 331, 1112, 2520, 2865
CspCI CAANNNNNGTGG 4 cut(s) 444, 479, 2776, 2811
CviAII CATG 7 cut(s) 526, 899, 946, 1357, 2524, 2668, 3052
CviQI GTAC 4 cut(s) 331, 1112, 2520, 2865
DraI TTTAAA 2 cut(s) 2776, 3325
DraIII CACNNNGTG 3 cut(s) 574, 1769, 2798
EaeI YGGCCR 1 cut(s) 2922
Eam1104I CTCTTC 4 cut(s) 42, 1905, 2349, 3012
EarI CTCTTC 4 cut(s) 42, 1905, 2349, 3012
Ecl136II GAGCTC 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 525, 1395
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 2 cut(s) 2135, 3396
Eco32I GATATC 1 cut(s) 2176
Eco47I GGWCC 2 cut(s) 592, 3614
Eco53kI GAGCTC 1 cut(s) 192
Eco57I CTGAAG 2 cut(s) 1281, 2051
Eco72I CACGTG 1 cut(s) 2795
Eco81I CCTNAGG 3 cut(s) 276, 2358, 2589
EcoICRI GAGCTC 1 cut(s) 192
EcoNI CCTNNNNNAGG 2 cut(s) 1140, 3621
EcoO109I RGGNCCY 1 cut(s) 3614
EcoRI GAATTC 1 cut(s) 3274
EcoRII CCWGG 7 cut(s) 87, 570, 641, 1048, 1627, 1966, 2626
EcoRV GATATC 1 cut(s) 2176
EcoT14I CCWWGG 2 cut(s) 525, 1395
EcoT22I ATGCAT 1 cut(s) 835
EcoT38I GRGCYC 1 cut(s) 194
ErhI CCWWGG 2 cut(s) 525, 1395
FaeI CATG 7 cut(s) 529, 902, 949, 1360, 2527, 2671, 3055
FalI AAGNNNNNCTT 2 cut(s) 1736, 1768
FaqI GGGAC 3 cut(s) 133, 1031, 1670
FatI CATG 7 cut(s) 525, 898, 945, 1356, 2523, 2667, 3051
FauI CCCGC 3 cut(s) 423, 1945, 3594
FauNDI CATATG 1 cut(s) 1470
FbaI TGATCA 1 cut(s) 2422
FblI GTMKAC 1 cut(s) 2900
FriOI GRGCYC 1 cut(s) 194
FspBI CTAG 6 cut(s) 1244, 1767, 2225, 2291, 2429, 2601
GlaI GCGC 2 cut(s) 212, 3644
GsaI CCCAGC 2 cut(s) 2300, 2579
GsuI CTGGAG 2 cut(s) 62, 110
HaeII RGCGCY 1 cut(s) 3646
HaeIII GGCC 6 cut(s) 1394, 1956, 2312, 2878, 2924, 3014
HapII CCGG 6 cut(s) 195, 291, 339, 2921, 3476, 3646
HgaI GACGC 1 cut(s) 2128
HhaI GCGC 2 cut(s) 213, 3645
Hin1II CATG 7 cut(s) 529, 902, 949, 1360, 2527, 2671, 3055
Hin6I GCGC 2 cut(s) 211, 3643
HinP1I GCGC 2 cut(s) 211, 3643
HincII GTYRAC 1 cut(s) 2901
HindII GTYRAC 1 cut(s) 2901
HpaII CCGG 6 cut(s) 195, 291, 339, 2921, 3476, 3646
HphI GGTGA 7 cut(s) 16, 292, 560, 623, 2083, 3017, 3027
HpyAV CCTTC 7 cut(s) 441, 963, 1256, 1326, 1701, 1741, 2075
HpyCH4IV ACGT 8 cut(s) 67, 150, 284, 326, 551, 2794, 3099, 3367
HpySE526I ACGT 8 cut(s) 67, 150, 284, 326, 551, 2794, 3099, 3367
Hsp92II CATG 7 cut(s) 529, 902, 949, 1360, 2527, 2671, 3055
HspAI GCGC 2 cut(s) 211, 3643
Kpn2I TCCGGA 1 cut(s) 290
KpnI GGTACC 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 2422
LmnI GCTCC 4 cut(s) 197, 1607, 2923, 3358
LweI GCATC 8 cut(s) 820, 842, 979, 2250, 2343, 2520, 2968, 3203
MabI ACCWGGT 1 cut(s) 570
MaeI CTAG 6 cut(s) 1244, 1767, 2225, 2291, 2429, 2601
MaeII ACGT 8 cut(s) 67, 150, 284, 326, 551, 2794, 3099, 3367
MfeI CAATTG 1 cut(s) 1571
MflI RGATCY 9 cut(s) 57, 366, 859, 1106, 1687, 2125, 2332, 2714, 3108
MhlI GDGCHC 3 cut(s) 194, 635, 1483
MlyI GAGTC 3 cut(s) 101, 572, 3092
MmeI TCCRAC 2 cut(s) 711, 1835
Mph1103I ATGCAT 1 cut(s) 835
MroI TCCGGA 1 cut(s) 290
MroXI GAANNNNTTC 2 cut(s) 1751, 2489
MslI CAYNNNNRTG 3 cut(s) 884, 2279, 2528
MspA1I CMGCKG 3 cut(s) 418, 1733, 2006
MspI CCGG 6 cut(s) 195, 291, 339, 2921, 3476, 3646
MspR9I CCNGG 9 cut(s) 89, 196, 339, 572, 643, 1050, 1629, 1968, 2628
MunI CAATTG 1 cut(s) 1571
Mva1269I GAATGC 4 cut(s) 1291, 1933, 2049, 2620
MvaI CCWGG 7 cut(s) 89, 572, 643, 1050, 1629, 1968, 2628
MvnI CGCG 3 cut(s) 211, 3400, 3485
NciI CCSGG 2 cut(s) 196, 339
NcoI CCATGG 1 cut(s) 525
NdeI CATATG 1 cut(s) 1470
NheI GCTAGC 1 cut(s) 2428
NlaIII CATG 7 cut(s) 529, 902, 949, 1360, 2527, 2671, 3055
NmuCI GTSAC 7 cut(s) 68, 280, 380, 1163, 1816, 3328, 3443
NruI TCGCGA 2 cut(s) 3400, 3485
NsiI ATGCAT 1 cut(s) 835
NspI RCATGY 2 cut(s) 949, 1360
NspV TTCGAA 2 cut(s) 1598, 2849
PaeI GCATGC 1 cut(s) 1360
PagI TCATGA 1 cut(s) 898
PasI CCCWGGG 1 cut(s) 1049
PctI GAATGC 4 cut(s) 1291, 1933, 2049, 2620
PdmI GAANNNNTTC 2 cut(s) 1751, 2489
PflMI CCANNNNNTGG 1 cut(s) 1769
PfoI TCCNGGA 1 cut(s) 87
PleI GAGTC 3 cut(s) 100, 571, 3091
PmaCI CACGTG 1 cut(s) 2795
PmlI CACGTG 1 cut(s) 2795
PpsI GAGTC 3 cut(s) 100, 571, 3091
Ppu21I YACGTR 3 cut(s) 68, 327, 2795
PpuMI RGGWCCY 1 cut(s) 3614
PshBI ATTAAT 2 cut(s) 1316, 2888
PsiI TTATAA 1 cut(s) 1844
Psp124BI GAGCTC 1 cut(s) 194
Psp5II RGGWCCY 1 cut(s) 3614
Psp6I CCWGG 7 cut(s) 87, 570, 641, 1048, 1627, 1966, 2626
PspCI CACGTG 1 cut(s) 2795
PspFI CCCAGC 2 cut(s) 2296, 2575
PspGI CCWGG 7 cut(s) 87, 570, 641, 1048, 1627, 1966, 2626
PspPI GGNCC 5 cut(s) 592, 1392, 1954, 2876, 3614
PspPPI RGGWCCY 1 cut(s) 3614
PstI CTGCAG 4 cut(s) 428, 1159, 1816, 3139
PstNI CAGNNNCTG 4 cut(s) 88, 668, 1736, 3617
PsuI RGATCY 9 cut(s) 57, 366, 859, 1106, 1687, 2125, 2332, 2714, 3108
PvuII CAGCTG 2 cut(s) 1733, 2006
RruI TCGCGA 2 cut(s) 3400, 3485
RsaI GTAC 4 cut(s) 332, 1113, 2521, 2866
RsaNI GTAC 4 cut(s) 331, 1112, 2520, 2865
RseI CAYNNNNRTG 3 cut(s) 884, 2279, 2528
SacI GAGCTC 1 cut(s) 194
SalI GTCGAC 1 cut(s) 2899
Sau96I GGNCC 5 cut(s) 592, 1392, 1954, 2876, 3614
SchI GAGTC 3 cut(s) 101, 572, 3092
ScrFI CCNGG 9 cut(s) 89, 196, 339, 572, 643, 1050, 1629, 1968, 2628
SduI GDGCHC 3 cut(s) 194, 635, 1483
SexAI ACCWGGT 1 cut(s) 570
SfaNI GCATC 8 cut(s) 820, 842, 979, 2250, 2343, 2520, 2968, 3203
SfcI CTRYAG 6 cut(s) 424, 535, 1155, 1812, 3135, 3207
SfuI TTCGAA 2 cut(s) 1598, 2849
SinI GGWCC 2 cut(s) 592, 3614
SmiMI CAYNNNNRTG 3 cut(s) 884, 2279, 2528
SmlI CTYRAG 3 cut(s) 1102, 3118, 3293
SmoI CTYRAG 3 cut(s) 1102, 3118, 3293
SphI GCATGC 1 cut(s) 1360
SsiI CCGC 8 cut(s) 29, 403, 416, 810, 1952, 3587, 3590, 3634
SspI AATATT 5 cut(s) 1019, 1532, 1549, 1801, 2892
SspMI CTAG 6 cut(s) 1244, 1767, 2225, 2291, 2429, 2601
SstI GAGCTC 1 cut(s) 194
StyD4I CCNGG 9 cut(s) 87, 194, 337, 570, 641, 1048, 1627, 1966, 2626
StyI CCWWGG 2 cut(s) 525, 1395
TaiI ACGT 8 cut(s) 70, 153, 287, 329, 554, 2797, 3102, 3370
TaqII GACCGA 1 cut(s) 3555
TatI WGTACW 2 cut(s) 1111, 2864
TauI GCSGC 1 cut(s) 3592
TscAI CASTG 5 cut(s) 167, 1414, 2283, 2425, 3559
TseFI GTSAC 7 cut(s) 68, 280, 380, 1163, 1816, 3328, 3443
Tsp45I GTSAC 7 cut(s) 68, 280, 380, 1163, 1816, 3328, 3443
TspGWI ACGGA 4 cut(s) 359, 1031, 1511, 2478
TspRI CASTG 5 cut(s) 167, 1414, 2283, 2425, 3559
Van91I CCANNNNNTGG 1 cut(s) 1769
VneI GTGCAC 1 cut(s) 631
VpaK11BI GGWCC 2 cut(s) 592, 3614
VspI ATTAAT 2 cut(s) 1316, 2888
XagI CCTNNNNNAGG 2 cut(s) 1140, 3621
XapI RAATTY 6 cut(s) 894, 1229, 1507, 1584, 2727, 3274
XbaI TCTAGA 3 cut(s) 1243, 2290, 2600
XceI RCATGY 2 cut(s) 949, 1360
XmiI GTMKAC 1 cut(s) 2900
XmnI GAANNNNTTC 2 cut(s) 1751, 2489
XspI CTAG 6 cut(s) 1244, 1767, 2225, 2291, 2429, 2601
Zsp2I ATGCAT 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.