AT2G31820

ankyrin repeat family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006581
Physical Location & Seq
Forward (+)
13529766 .. 13533348
3583 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G31820.1

Sequence Viewer

Length: 1989 bp
ATGACTATAATGGAGGAGAAGCAGAACAACAGTCCTGTGGGAGAGAAGCAACAGAGCTTTAATGGTATAATAATAAACCAAACACAAGAGAATCAGACAAAATCAATGGAGAAACAACAGAGTTTCCGATGTGTGGTGGAGAATCAACCGAGAAAGGCGAGAGCTTTAGAGAAACAAGTGAGTTTCCAAGGTGTGAATGTAGAGAACTATCAGCAAAGCAGACTCGGAAGATCAATGGAGAAGCAACAGAGTTTTCGAGGTGTCAACGTTGAGAATCATAAACGTGGAGTTATGGAGAAGCTACCGAGTTTTGGTAGAGCAACAATGGAGAGGCAGAAGAGTTTCCGTGGTGGGTTTTTGGAGAAGCAGAAGAGCTTCCGTGTGGTGATGGAGAGGCAATTGAGTTTTATAGGTGAGAGGAGGAAGAAGAATGAATCACCAGGGAAAAGAGGAGACTCTTCTCTTCATATCGCTGCGAGAACCGGGAACTTAAGTAAGGTTAAAGAACTGATTCGAGGTTGTGGTGATGAGTTGAAAGAGTTGTTGTCAAAGCAAAATCTTGAAGGAGAGACTCCTCTTTATACTGCAGCAGAGAATGGGCATTCAATTGTTGTTGAGGAGATGTTGAAGCATATGGACCTTGAAACTGCTTCCATCGCTGCTAGAAACGGGTTTGATCCATTTCATGTCGCCGCCAAACAAGGCCATCTCGAGGTGTTGAAGATACTGTTGGAGACATTCCCTAATTTGGCAATGACAACGGATTTATCGTGTACAACTGCGTTACACACTGCTGCAACACAGGGACATATTGATGTGGTGAATCTTCTTTTGGAGACAGACTCTAATTTGGCTAAGATTGCTAAGAACAACGGTAAAACTGCGCTTCACTCTGCAGCAAGGATGGGTCATGTGGAAGTTGTTAAATCTCTGATAGGTAAGGATCCAAGCATTGGGTTTAGAACCGATAAAAAAGGGCAAACTGCTCTTCACATGGCTGTAAAAGGTCAAAATGATGGGATTGTTGTTGAGTTGGTGAAACCTGATGTTGCTGTTTTGAGCGTTGAGGATAATAAAGGAAATACACCATTGCACATTGCCACAAACAAGGGGCGTATTAAGATAGTACGGTGTTTGGTATCTTTTGAAGGCATAAACCTCAACCCAATAAACAAAGCTGGAGATACGCCACTCGATGTCTCTGAGAAGATAGGAAACGCAGAGCTTGTGTCAGTTCTGAAGGAAGCAGGAGCTGCTACAGCTAAAGATCTCGGAAAGCCTCAGAACCCAGCTAAGCAACTGAAGCAAACAGTCAGCGACATCAAACATGAAGTACAATCTCAGCTTCAGCAATCCAGACAAACAGGTGTAAGAGTCCAGAAGATAGCAAAAAGACTCAAGAAGCTACACATTAGTGGTCTAAACAACGCTATAAACTCAGCAACTGTTGTGGCAGTGCTTATTGCCACAGTGGCTTTCGCAGCAATCTTCACAATACCCGGTCAGTACGAAGAGGACCGGTCGAAAGGAGAGTTGCTAGGACAAGCTCATATAGCAAACAAAGCACCGTTTCTGGTCTTCTTCATCTTTGACAGCTTGGCACTGTTTATATCCTTAGCTGTTGTTGTGGTGCAGACTTCAGTTGTTGTGATTGAGCAGAAGGCGAAAAAGAAGCTTGTGTTTGTAATCAACAAGCTCATGTGGTGTGCTTGTCTGTTCATATCCATTGCCTTTGTTTCTCTCTCTTATATTGTTGTTGGCAAAGAGGAGATGTGGTTGGCTGTTTGCGCGACTGTTATCGGCGGCACGATTATGTTGACTACGATTGGTGCGATGTGCTATTGTGTGGTCATGCATAGGATGGAAGAATCTAAATTGAGGAGCATAAGGAAAGAAAGAAGCAAGTCTCAGTCTTTCTCTATGTCTCGTATGCCGTCTGATTCAGATATTCTAAATGGTGAATACAATAAGAGAATGTATGCCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 PGG

Protein Analysis

662

Amino Acids

73.14

Weight (kDa)

9.51

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 155 - 245 2e-15 Ankyrin repeats (3 copies)
Ank_4 PF13637 229 - 278 4.9e-11 Ankyrin repeats (many copies)
Ank_2 PF12796 259 - 315 3.7e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 262 - 312 3.7e-10 Ankyrin repeats (many copies)
Ank PF00023 291 - 317 8e-06 Ankyrin repeat
Ank_4 PF13637 296 - 338 5.7e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 322 - 388 4.4e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 354 - 418 4e-08 Ankyrin repeats (3 copies)
PGG PF13962 473 - 585 4.9e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 953
AccII CGCG 1 cut(s) 1790
AciI CCGC 2 cut(s) 693, 1803
AclI AACGTT 1 cut(s) 267
AclWI GGATC 3 cut(s) 671, 938, 951
AcuI CTGAAG 4 cut(s) 1259, 1322, 1331, 1623
AfaI GTAC 4 cut(s) 775, 1128, 1335, 1508
AfiI CCNNNNNNNGG 5 cut(s) 133, 311, 712, 748, 953
AflII CTTAAG 1 cut(s) 490
AgeI ACCGGT 1 cut(s) 1518
AgsI TTSAA 7 cut(s) 535, 563, 606, 628, 644, 721, 1148
AjnI CCWGG 1 cut(s) 439
AjuI GAANNNNNNNTTGG 4 cut(s) 713, 745, 815, 847
AleI CACNNNNGTG 1 cut(s) 1413
Alw26I GTCTC 7 cut(s) 447, 563, 728, 830, 1204, 1911, 1929
AlwI GGATC 3 cut(s) 671, 938, 951
AlwNI CAGNNNCTG 2 cut(s) 1253, 1445
Ama87I CYCGRG 1 cut(s) 710
AoxI GGCC 1 cut(s) 703
ApeKI GCWGC 7 cut(s) 473, 587, 659, 794, 896, 1253, 1481
AsiGI ACCGGT 1 cut(s) 1518
Asp700I GAANNNNTTC 3 cut(s) 341, 374, 510
AspLEI GCGC 2 cut(s) 886, 1790
AspS9I GGNCC 2 cut(s) 637, 1516
AsuC2I CCSGG 2 cut(s) 484, 1500
AsuHPI GGTGA 7 cut(s) 397, 425, 429, 536, 832, 1048, 1970
AvaI CYCGRG 1 cut(s) 710
AvaII GGWCC 2 cut(s) 637, 1516
BamHI GGATCC 1 cut(s) 943
BbsI GAAGAC 1 cut(s) 1570
BbvI GCAGC 7 cut(s) 460, 599, 646, 781, 908, 1240, 1493
BccI CCATC 6 cut(s) 382, 662, 714, 898, 1010, 1855
BceAI ACGGC 1 cut(s) 1918
BciT130I CCWGG 1 cut(s) 441
BcnI CCSGG 2 cut(s) 484, 1500
BcoDI GTCTC 7 cut(s) 447, 563, 728, 830, 1204, 1911, 1929
BfaI CTAG 2 cut(s) 663, 1538
BfmI CTRYAG 3 cut(s) 585, 894, 1257
BfrI CTTAAG 1 cut(s) 490
BglI GCCNNNNNGGC 1 cut(s) 1472
BglII AGATCT 1 cut(s) 1267
BisI GCNGC 9 cut(s) 474, 588, 660, 693, 795, 897, 1254, 1482, 1804
BlpI GCTNAGC 1 cut(s) 1293
BlsI GCNGC 9 cut(s) 475, 589, 661, 694, 796, 898, 1255, 1483, 1805
Bme1390I CCNGG 3 cut(s) 441, 484, 1500
Bme18I GGWCC 2 cut(s) 637, 1516
BmeT110I CYCGRG 1 cut(s) 710
BmgT120I GGNCC 2 cut(s) 637, 1516
BmiI GGNNCC 1 cut(s) 945
BmrFI CCNGG 3 cut(s) 441, 484, 1500
BpiI GAAGAC 1 cut(s) 1570
BplI GAGNNNNNCTC 2 cut(s) 827, 859
BpmI CTGGAG 1 cut(s) 1200
Bpu10I CCTNAGC 1 cut(s) 1615
Bpu1102I GCTNAGC 1 cut(s) 1293
BpuEI CTTGAG 1 cut(s) 1382
BpuMI CCSGG 2 cut(s) 484, 1500
BsaJI CCNNGG 3 cut(s) 187, 346, 440
BsaWI WCCGGW 1 cut(s) 1518
BsaXI ACNNNNNCTCC 2 cut(s) 1760, 1790
Bsc4I CCNNNNNNNGG 5 cut(s) 133, 311, 712, 748, 953
Bse118I RCCGGY 1 cut(s) 1518
Bse3DI GCAATG 4 cut(s) 759, 1088, 1095, 1725
BseBI CCWGG 1 cut(s) 441
BseDI CCNNGG 3 cut(s) 187, 346, 440
BseGI GGATG 2 cut(s) 909, 1866
BseLI CCNNNNNNNGG 5 cut(s) 133, 311, 712, 748, 953
BseMI GCAATG 4 cut(s) 759, 1088, 1095, 1725
BseMII CTCAG 5 cut(s) 1194, 1295, 1355, 1452, 1922
BseRI GAGGAG 7 cut(s) 29, 433, 465, 564, 632, 1781, 1894
BseXI GCAGC 7 cut(s) 460, 599, 646, 781, 908, 1240, 1493
BseYI CCCAGC 1 cut(s) 1288
BsgI GTGCAG 1 cut(s) 1652
Bsh1236I CGCG 1 cut(s) 1790
Bsh1285I CGRYCG 1 cut(s) 1523
BshFI GGCC 1 cut(s) 705
BshTI ACCGGT 1 cut(s) 1518
BsiEI CGRYCG 1 cut(s) 1523
BsiHKCI CYCGRG 1 cut(s) 710
BsiSI CCGG 3 cut(s) 483, 1500, 1519
BslFI GGGAC 1 cut(s) 819
BslI CCNNNNNNNGG 5 cut(s) 133, 311, 712, 748, 953
BsmAI GTCTC 7 cut(s) 447, 563, 728, 830, 1204, 1911, 1929
BsmFI GGGAC 1 cut(s) 819
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 1 cut(s) 705
BsoBI CYCGRG 1 cut(s) 710
Bsp1407I TGTACA 1 cut(s) 773
Bsp143I GATC 4 cut(s) 230, 676, 943, 1267
Bsp1720I GCTNAGC 1 cut(s) 1293
BspACI CCGC 2 cut(s) 693, 1803
BspANI GGCC 1 cut(s) 705
BspCNI CTCAG 5 cut(s) 1195, 1294, 1354, 1451, 1921
BspFNI CGCG 1 cut(s) 1790
BspLI GGNNCC 1 cut(s) 945
BspMAI CTGCAG 2 cut(s) 589, 898
BspPI GGATC 3 cut(s) 671, 938, 951
BspQI GCTCTTC 2 cut(s) 365, 993
BspTI CTTAAG 1 cut(s) 490
BsrDI GCAATG 4 cut(s) 759, 1088, 1095, 1725
BsrFI RCCGGY 1 cut(s) 1518
BsrGI TGTACA 1 cut(s) 773
BssAI RCCGGY 1 cut(s) 1518
BssECI CCNNGG 3 cut(s) 187, 346, 440
BssMI GATC 4 cut(s) 230, 676, 943, 1267
BssT1I CCWWGG 1 cut(s) 187
Bst2UI CCWGG 1 cut(s) 441
Bst6I CTCTTC 6 cut(s) 332, 365, 463, 468, 993, 1506
BstAFI CTTAAG 1 cut(s) 490
BstAPI GCANNNNNTGC 1 cut(s) 1253
BstAUI TGTACA 1 cut(s) 773
BstDEI CTNAG 9 cut(s) 855, 864, 1203, 1281, 1293, 1341, 1438, 1615, 1908
BstDSI CCRYGG 1 cut(s) 346
BstF5I GGATG 2 cut(s) 909, 1866
BstFNI CGCG 1 cut(s) 1790
BstHHI GCGC 2 cut(s) 886, 1790
BstKTI GATC 4 cut(s) 233, 679, 946, 1270
BstMAI GTCTC 7 cut(s) 447, 563, 728, 830, 1204, 1911, 1929
BstMBI GATC 4 cut(s) 230, 676, 943, 1267
BstMCI CGRYCG 1 cut(s) 1523
BstNI CCWGG 1 cut(s) 441
BstSCI CCNGG 3 cut(s) 439, 482, 1498
BstSFI CTRYAG 3 cut(s) 585, 894, 1257
BstUI CGCG 1 cut(s) 1790
BstV1I GCAGC 7 cut(s) 460, 599, 646, 781, 908, 1240, 1493
BstV2I GAAGAC 1 cut(s) 1570
BstX2I RGATCY 2 cut(s) 943, 1267
BstYI RGATCY 2 cut(s) 943, 1267
BsuRI GGCC 1 cut(s) 705
BtgI CCRYGG 1 cut(s) 346
BtgZI GCGATG 2 cut(s) 640, 1847
BtsCI GGATG 2 cut(s) 909, 1866
BtsI GCAGTG 2 cut(s) 789, 1461
BtsIMutI CAGTG 4 cut(s) 789, 1461, 1476, 1601
CaiI CAGNNNCTG 2 cut(s) 1253, 1445
CfoI GCGC 2 cut(s) 886, 1790
Cfr10I RCCGGY 1 cut(s) 1518
Cfr13I GGNCC 2 cut(s) 637, 1516
Csp6I GTAC 4 cut(s) 774, 1127, 1334, 1507
CspAI ACCGGT 1 cut(s) 1518
CspCI CAANNNNNGTGG 2 cut(s) 1431, 1466
CviAII CATG 6 cut(s) 686, 911, 994, 1328, 1699, 1852
CviQI GTAC 4 cut(s) 774, 1127, 1334, 1507
DdeI CTNAG 9 cut(s) 855, 864, 1203, 1281, 1293, 1341, 1438, 1615, 1908
DpnI GATC 4 cut(s) 232, 678, 945, 1269
DpnII GATC 4 cut(s) 230, 676, 943, 1267
Eam1104I CTCTTC 6 cut(s) 332, 365, 463, 468, 993, 1506
EarI CTCTTC 6 cut(s) 332, 365, 463, 468, 993, 1506
Eco130I CCWWGG 1 cut(s) 187
Eco47I GGWCC 2 cut(s) 637, 1516
Eco57I CTGAAG 4 cut(s) 1259, 1322, 1331, 1623
Eco88I CYCGRG 1 cut(s) 710
EcoRII CCWGG 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 187
EcoT22I ATGCAT 1 cut(s) 1857
ErhI CCWWGG 1 cut(s) 187
FaeI CATG 6 cut(s) 689, 914, 997, 1331, 1702, 1855
FaqI GGGAC 1 cut(s) 819
FatI CATG 6 cut(s) 685, 910, 993, 1327, 1698, 1851
FauNDI CATATG 1 cut(s) 633
Fnu4HI GCNGC 9 cut(s) 474, 588, 660, 693, 795, 897, 1254, 1482, 1804
FokI GGATG 2 cut(s) 916, 1873
Fsp4HI GCNGC 9 cut(s) 474, 588, 660, 693, 795, 897, 1254, 1482, 1804
FspBI CTAG 2 cut(s) 663, 1538
GlaI GCGC 2 cut(s) 885, 1789
GluI GCNGC 9 cut(s) 474, 588, 660, 693, 795, 897, 1254, 1482, 1804
GsaI CCCAGC 1 cut(s) 1292
GsuI CTGGAG 1 cut(s) 1200
HaeIII GGCC 1 cut(s) 705
HapII CCGG 3 cut(s) 483, 1500, 1519
HhaI GCGC 2 cut(s) 886, 1790
Hin1II CATG 6 cut(s) 689, 914, 997, 1331, 1702, 1855
Hin6I GCGC 2 cut(s) 884, 1788
HinP1I GCGC 2 cut(s) 884, 1788
HincII GTYRAC 2 cut(s) 265, 1818
HindII GTYRAC 2 cut(s) 265, 1818
HindIII AAGCTT 1 cut(s) 1673
HpaII CCGG 3 cut(s) 483, 1500, 1519
HphI GGTGA 7 cut(s) 397, 425, 429, 536, 832, 1048, 1970
Hpy166II GTNNAC 3 cut(s) 265, 774, 1818
Hpy188III TCNNGA 5 cut(s) 560, 710, 1356, 1378, 1399
Hpy8I GTNNAC 3 cut(s) 265, 774, 1818
HpyAV CCTTC 4 cut(s) 557, 1142, 1234, 1654
HpyCH4IV ACGT 2 cut(s) 267, 283
HpyCH4V TGCA 6 cut(s) 587, 797, 896, 1093, 1633, 1855
HpyF3I CTNAG 9 cut(s) 855, 864, 1203, 1281, 1293, 1341, 1438, 1615, 1908
HpySE526I ACGT 2 cut(s) 267, 283
Hsp92II CATG 6 cut(s) 689, 914, 997, 1331, 1702, 1855
HspAI GCGC 2 cut(s) 884, 1788
Kzo9I GATC 4 cut(s) 230, 676, 943, 1267
LguI GCTCTTC 2 cut(s) 365, 993
LmnI GCTCC 2 cut(s) 1250, 1881
Lsp1109I GCAGC 7 cut(s) 460, 599, 646, 781, 908, 1240, 1493
MaeI CTAG 2 cut(s) 663, 1538
MaeII ACGT 2 cut(s) 267, 283
MaeIII GTNAC 1 cut(s) 783
MalI GATC 4 cut(s) 232, 678, 945, 1269
MboI GATC 4 cut(s) 230, 676, 943, 1267
MfeI CAATTG 2 cut(s) 398, 606
MflI RGATCY 2 cut(s) 943, 1267
MluCI AATT 5 cut(s) 398, 606, 745, 847, 1874
MlyI GAGTC 6 cut(s) 216, 449, 565, 836, 1383, 1389
MmeI TCCRAC 1 cut(s) 711
Mph1103I ATGCAT 1 cut(s) 1857
MroXI GAANNNNTTC 3 cut(s) 341, 374, 510
MseI TTAA 5 cut(s) 60, 491, 501, 924, 1119
MslI CAYNNNNRTG 4 cut(s) 282, 813, 1413, 1811
MspCI CTTAAG 1 cut(s) 490
MspI CCGG 3 cut(s) 483, 1500, 1519
MspR9I CCNGG 3 cut(s) 441, 484, 1500
MunI CAATTG 2 cut(s) 398, 606
Mva1269I GAATGC 1 cut(s) 601
MvaI CCWGG 1 cut(s) 441
MvnI CGCG 1 cut(s) 1790
NciI CCSGG 2 cut(s) 484, 1500
NdeI CATATG 1 cut(s) 633
NdeII GATC 4 cut(s) 230, 676, 943, 1267
NlaIII CATG 6 cut(s) 689, 914, 997, 1331, 1702, 1855
NlaIV GGNNCC 1 cut(s) 945
NsiI ATGCAT 1 cut(s) 1857
OliI CACNNNNGTG 1 cut(s) 1413
PaeR7I CTCGAG 1 cut(s) 710
PciSI GCTCTTC 2 cut(s) 365, 993
PcsI WCGNNNNNNNCGW 2 cut(s) 767, 1829
PctI GAATGC 1 cut(s) 601
PdmI GAANNNNTTC 3 cut(s) 341, 374, 510
PfeI GAWTC 8 cut(s) 91, 142, 274, 434, 511, 823, 1868, 1940
PflMI CCANNNNNTGG 1 cut(s) 953
PinAI ACCGGT 1 cut(s) 1518
PkrI GCNGC 9 cut(s) 475, 589, 661, 694, 796, 898, 1255, 1483, 1805
PleI GAGTC 6 cut(s) 216, 449, 565, 836, 1382, 1389
PpsI GAGTC 6 cut(s) 216, 449, 565, 836, 1382, 1389
Psp1406I AACGTT 1 cut(s) 267
Psp6I CCWGG 1 cut(s) 439
PspFI CCCAGC 1 cut(s) 1288
PspGI CCWGG 1 cut(s) 439
PspN4I GGNNCC 1 cut(s) 945
PspPI GGNCC 2 cut(s) 637, 1516
PstI CTGCAG 2 cut(s) 589, 898
PstNI CAGNNNCTG 2 cut(s) 1253, 1445
PsuI RGATCY 2 cut(s) 943, 1267
RsaI GTAC 4 cut(s) 775, 1128, 1335, 1508
RsaNI GTAC 4 cut(s) 774, 1127, 1334, 1507
RseI CAYNNNNRTG 4 cut(s) 282, 813, 1413, 1811
SapI GCTCTTC 2 cut(s) 365, 993
SaqAI TTAA 5 cut(s) 60, 491, 501, 924, 1119
SatI GCNGC 9 cut(s) 474, 588, 660, 693, 795, 897, 1254, 1482, 1804
Sau3AI GATC 4 cut(s) 230, 676, 943, 1267
Sau96I GGNCC 2 cut(s) 637, 1516
SchI GAGTC 6 cut(s) 216, 449, 565, 836, 1383, 1389
ScrFI CCNGG 3 cut(s) 441, 484, 1500
SfcI CTRYAG 3 cut(s) 585, 894, 1257
Sfr274I CTCGAG 1 cut(s) 710
SinI GGWCC 2 cut(s) 637, 1516
SlaI CTCGAG 1 cut(s) 710
SmiMI CAYNNNNRTG 4 cut(s) 282, 813, 1413, 1811
SmlI CTYRAG 3 cut(s) 490, 710, 1397
SmoI CTYRAG 3 cut(s) 490, 710, 1397
Sse9I AATT 5 cut(s) 398, 606, 745, 847, 1874
SsiI CCGC 2 cut(s) 693, 1803
SspMI CTAG 2 cut(s) 663, 1538
StyD4I CCNGG 3 cut(s) 439, 482, 1498
StyI CCWWGG 1 cut(s) 187
TaiI ACGT 2 cut(s) 270, 286
TaqI TCGA 5 cut(s) 256, 514, 711, 1194, 1523
TasI AATT 5 cut(s) 398, 606, 745, 847, 1874
TatI WGTACW 2 cut(s) 773, 1333
TauI GCSGC 2 cut(s) 695, 1806
TfiI GAWTC 8 cut(s) 91, 142, 274, 434, 511, 823, 1868, 1940
Tru1I TTAA 5 cut(s) 60, 491, 501, 924, 1119
Tru9I TTAA 5 cut(s) 60, 491, 501, 924, 1119
TscAI CASTG 4 cut(s) 796, 1461, 1476, 1608
TseI GCWGC 7 cut(s) 473, 587, 659, 794, 896, 1253, 1481
TspDTI ATGAA 6 cut(s) 447, 455, 674, 1344, 1573, 1708
TspGWI ACGGA 3 cut(s) 335, 368, 776
TspRI CASTG 4 cut(s) 796, 1461, 1476, 1608
Van91I CCANNNNNTGG 1 cut(s) 953
Vha464I CTTAAG 1 cut(s) 490
VpaK11BI GGWCC 2 cut(s) 637, 1516
XhoI CTCGAG 1 cut(s) 710
XmnI GAANNNNTTC 3 cut(s) 341, 374, 510
XspI CTAG 2 cut(s) 663, 1538
Zsp2I ATGCAT 1 cut(s) 1857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.