MD15G1143700.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006581
Physical Location & Seq
Reverse (-)
10601098 .. 10604809
3712 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1143700.v1.1.491

Sequence Viewer

Length: 1761 bp
ATGGAGAGGCAGAGCAGTTTTCAGGCTCCAAGGATGGAAAAACTACCAAGTTTCAGTGGGGCAATCGAGCAACAGTCAAGTTCTCGAGGAGCGATGGAAAAACAGAAGAGTTTTCGCGGATTTATGGAGAAGCAGAAGAGTTTTCGGATAGTTATGGAGAAGCAGCTGAGCTTTATTGGCGGTGAACGGAGGAGGAGCAAAGAGTCACCGGGTAAAAGAGGTGACTCCCAACTCCATTTGGCGGCTCGAGCAGGGAATTTAGTTAGAGTCAGAGAAATTCTTCAGAATTGTAACAGCACTGAGTCAATTGGTTTATTGTCGAAGATGAACCAGGAGGGAGAGACCCCCCTCTATGCCTCAGCGGAGAATGGGCATGCTGAGGTTGTTGGGGAAATGTTGAAGCACATGGACCTTCAAACTGCATCCATCGCTGCCAGAAATGGCTACGACCCATTCCACATTGCTGTAAGACATGGCCATCTTGAGGTGTTGAAAGAACTTCTGCACGTGTTCCCAAACTTGGCAATGACCACAGATCTATCGAATTCAACCGCCTTACACACGGCTGCTACTCAGGGGCATATTAATATTGTAAATCTCCTTTTGGAAACTGACTCAAATCTTGCCAAGATAGCCCGCAATAATGGGAAGACTGTGCTTCATTCAGCAGCAAGGTTGGGGCACTTGGAAGTAGTCAAGTCCTTTCTACGCAAGGATCCAAGTGCTGCTTTTAGAACTGATCTTAAAGGCCAAACTGCGCTACACATGGCCGTGAAGGGGCACAATGAGGAGATTGTGCTGGAGTTGCTGAAACCCGACCCCTCAGTTTTGACTGTGGAAGATAACAAGGGAAACAATGCATTGCATATTGCCACAAGGAAGGGCCGTATTGAGAATGTATGCCGTCTGTTGTCCATAAATAAAGAGGCTAGTAACATTAATGCAAGCAACAAGGCTGGAGAGACCCCCCTTGACATTGCAGAAAAATATGGGAGTCCAGAACTTGTGGCCGTGTTAAAGGAAGCAGGGGCCACCAATTCTAAAGACCAAGGAAAACCTGCAAATCCAGCAAAGCAGCTGAAGCAGACCGTGAGTGACATAAAGCACGATGTCCAATCCCAACTTCAACAGACCCGCCAAACTGGTGCCAGAGTCCAGAAAATTGCAAAAAAGTTGAAAAAGCTCCACATTAGCGGCCTCAACAATGCTATAAACAATGCCACTATTGTTGCCGTGCTGATTGCCACCGTTGCTTTTGCGGCCATATTCACAGTTCCTGGCCAATATGTTGAGGAAGAAACGCCAGGGCTTACGCTTGGACAAGCGCACATAGCAAATAATGCCGCTTTCATCATCTTCTTTGTATTTGACAGCCTGGCGCTCTTCATCTCCCTGGCTGTTGTGGTAGTCCAGACATCTGTGGTTGTGATTGAGCAGAAGGCAAAAAGGCAGCTTGTGTTTGTGATCAACAAGCTCATGTGGCTAGCTTGCCTCTTCATTTCGATTGCTTTTATTTCTCTGACGTATGTGGTGGTGGGCTCACACTCGAGGTGGCTCGCAGTGTATGCAACAGTTATAGGCAGCACAATCATGCTTACCACCATTGGCTCCATGTGCTATTGTGTCATTTTACATAGGATGGAGGAGAGCAAAATGAGGAACATCCGGAGAGCTGAGAGTAAGTCTCGTTCGTTGTCTATGTCTATGGCATCAGAACACGAGATTTTGAACAGCGAATATAAGAGGATGTATGCGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 PGG

Protein Analysis

587

Amino Acids

64.53

Weight (kDa)

9.56

Isoelectric Point (pI)

49.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 73 - 135 5.7e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 78 - 169 5.1e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 153 - 202 3.3e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 180 - 238 6.1e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 186 - 233 6.7e-07 Ankyrin repeats (many copies)
Ank_4 PF13637 220 - 266 3e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 227 - 306 5.2e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 280 - 327 8.1e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 281 - 345 7.6e-07 Ankyrin repeats (3 copies)
PGG PF13962 399 - 511 1.1e-27 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1066
AccB1I GGYRCC 1 cut(s) 1145
AccII CGCG 1 cut(s) 117
AccIII TCCGGA 1 cut(s) 1665
AclWI GGATC 2 cut(s) 710, 723
AcoI YGGCCR 5 cut(s) 475, 768, 1008, 1260, 1279
AcsI RAATTY 3 cut(s) 256, 276, 544
AcuI CTGAAG 2 cut(s) 266, 1100
AcvI CACGTG 1 cut(s) 508
AfiI CCNNNNNNNGG 4 cut(s) 241, 484, 520, 777
AflIII ACRYGT 1 cut(s) 507
AgsI TTSAA 7 cut(s) 400, 416, 493, 549, 1127, 1177, 1729
AjnI CCWGG 5 cut(s) 330, 1276, 1303, 1374, 1392
AjuI GAANNNNNNNTTGG 2 cut(s) 1107, 1139
AluBI AGCT 8 cut(s) 166, 171, 1078, 1183, 1453, 1474, 1487, 1673
AluI AGCT 8 cut(s) 166, 171, 1078, 1183, 1453, 1474, 1487, 1673
Alw26I GTCTC 3 cut(s) 335, 956, 1689
AlwI GGATC 2 cut(s) 710, 723
AlwNI CAGNNNCTG 1 cut(s) 1277
Ama87I CYCGRG 3 cut(s) 84, 246, 1546
Aor13HI TCCGGA 1 cut(s) 1665
AoxI GGCC 9 cut(s) 475, 748, 768, 883, 1008, 1029, 1195, 1260, 1279
ApeKI GCWGC 8 cut(s) 163, 431, 566, 668, 725, 1075, 1450, 1581
ApoI RAATTY 3 cut(s) 256, 276, 544
AseI ATTAAT 2 cut(s) 585, 939
Asp700I GAANNNNTTC 1 cut(s) 279
AspLEI GCGC 4 cut(s) 760, 1327, 1381, 1756
AspS9I GGNCC 3 cut(s) 409, 883, 1029
AsuC2I CCSGG 1 cut(s) 210
AsuHPI GGTGA 3 cut(s) 194, 198, 233
AsuNHI GCTAGC 1 cut(s) 1483
AvaI CYCGRG 3 cut(s) 84, 246, 1546
AvaII GGWCC 1 cut(s) 409
BaeGI GKGCMC 2 cut(s) 684, 783
BalI TGGCCA 2 cut(s) 477, 1281
BamHI GGATCC 1 cut(s) 715
BanI GGYRCC 1 cut(s) 1145
BanII GRGCYC 1 cut(s) 1541
BauI CACGAG 1 cut(s) 1718
BbrPI CACGTG 1 cut(s) 508
BbsI GAAGAC 1 cut(s) 656
BbvCI CCTCAGC 2 cut(s) 358, 378
BbvI GCAGC 8 cut(s) 175, 418, 553, 680, 712, 1087, 1462, 1593
BccI CCATC 5 cut(s) 28, 88, 434, 486, 1633
BceAI ACGGC 6 cut(s) 579, 755, 870, 888, 995, 1217
BciT130I CCWGG 5 cut(s) 332, 1278, 1305, 1376, 1394
BclI TGATCA 1 cut(s) 1464
BcnI CCSGG 1 cut(s) 210
BcoDI GTCTC 3 cut(s) 335, 956, 1689
BfaI CTAG 2 cut(s) 930, 1484
BfoI RGCGCY 1 cut(s) 1382
BfuAI ACCTGC 1 cut(s) 1066
BglII AGATCT 1 cut(s) 535
BlpI GCTNAGC 1 cut(s) 167
Bme1390I CCNGG 6 cut(s) 210, 332, 1278, 1305, 1376, 1394
Bme18I GGWCC 1 cut(s) 409
BmeT110I CYCGRG 3 cut(s) 84, 246, 1546
BmgT120I GGNCC 3 cut(s) 409, 883, 1029
BmiI GGNNCC 5 cut(s) 27, 717, 1030, 1147, 1609
BmrFI CCNGG 6 cut(s) 210, 332, 1278, 1305, 1376, 1394
BmsI GCATC 2 cut(s) 431, 1718
BmtI GCTAGC 1 cut(s) 1487
BpiI GAAGAC 1 cut(s) 656
BplI GAGNNNNNCTC 2 cut(s) 1669, 1701
BpmI CTGGAG 2 cut(s) 821, 978
Bpu10I CCTNAGC 2 cut(s) 358, 378
Bpu1102I GCTNAGC 1 cut(s) 167
BpuEI CTTGAG 1 cut(s) 503
BpuMI CCSGG 1 cut(s) 210
BsaAI YACGTR 1 cut(s) 508
BsaI GGTCTC 2 cut(s) 335, 956
BsaJI CCNNGG 4 cut(s) 29, 1048, 1304, 1392
BsaWI WCCGGW 1 cut(s) 1665
Bsc4I CCNNNNNNNGG 4 cut(s) 241, 484, 520, 777
Bse1I ACTGG 1 cut(s) 1147
Bse3DI GCAATG 4 cut(s) 459, 531, 860, 975
BseAI TCCGGA 1 cut(s) 1665
BseBI CCWGG 5 cut(s) 332, 1278, 1305, 1376, 1394
BseDI CCNNGG 4 cut(s) 29, 1048, 1304, 1392
BseGI GGATG 5 cut(s) 39, 422, 1644, 1662, 1752
BseLI CCNNNNNNNGG 4 cut(s) 241, 484, 520, 777
BseMI GCAATG 4 cut(s) 459, 531, 860, 975
BseMII CTCAG 7 cut(s) 158, 291, 369, 372, 587, 837, 1665
BseNI ACTGG 1 cut(s) 1147
BseRI GAGGAG 5 cut(s) 102, 205, 208, 803, 1658
BseSI GKGCMC 2 cut(s) 684, 783
BseXI GCAGC 8 cut(s) 175, 418, 553, 680, 712, 1087, 1462, 1593
BsgI GTGCAG 1 cut(s) 488
Bsh1236I CGCG 1 cut(s) 117
BshFI GGCC 9 cut(s) 477, 750, 770, 885, 1010, 1031, 1197, 1262, 1281
BshNI GGYRCC 1 cut(s) 1145
BsiHKCI CYCGRG 3 cut(s) 84, 246, 1546
BsiSI CCGG 2 cut(s) 209, 1666
BslI CCNNNNNNNGG 4 cut(s) 241, 484, 520, 777
BsmAI GTCTC 3 cut(s) 335, 956, 1689
BsnI GGCC 9 cut(s) 477, 750, 770, 885, 1010, 1031, 1197, 1262, 1281
Bso31I GGTCTC 2 cut(s) 335, 956
BsoBI CYCGRG 3 cut(s) 84, 246, 1546
Bsp1286I GDGCHC 3 cut(s) 684, 783, 1541
Bsp13I TCCGGA 1 cut(s) 1665
Bsp143I GATC 4 cut(s) 535, 715, 739, 1464
Bsp1720I GCTNAGC 1 cut(s) 167
BspANI GGCC 9 cut(s) 477, 750, 770, 885, 1010, 1031, 1197, 1262, 1281
BspCNI CTCAG 7 cut(s) 159, 292, 370, 371, 586, 836, 1666
BspEI TCCGGA 1 cut(s) 1665
BspFNI CGCG 1 cut(s) 117
BspLI GGNNCC 5 cut(s) 27, 717, 1030, 1147, 1609
BspMI ACCTGC 1 cut(s) 1066
BspOI GCTAGC 1 cut(s) 1487
BspPI GGATC 2 cut(s) 710, 723
BspQI GCTCTTC 1 cut(s) 1388
BspT107I GGYRCC 1 cut(s) 1145
BspTNI GGTCTC 2 cut(s) 335, 956
BsrDI GCAATG 4 cut(s) 459, 531, 860, 975
BsrI ACTGG 1 cut(s) 1147
BssECI CCNNGG 4 cut(s) 29, 1048, 1304, 1392
BssMI GATC 4 cut(s) 535, 715, 739, 1464
BssSI CACGAG 1 cut(s) 1718
BssT1I CCWWGG 2 cut(s) 29, 1048
Bst2BI CACGAG 1 cut(s) 1718
Bst2UI CCWGG 5 cut(s) 332, 1278, 1305, 1376, 1394
Bst4CI ACNGT 7 cut(s) 75, 655, 835, 1090, 1249, 1273, 1573
Bst6I CTCTTC 4 cut(s) 101, 131, 1388, 1499
BstAPI GCANNNNNTGC 2 cut(s) 1340, 1565
BstBAI YACGTR 1 cut(s) 508
BstC8I GCNNGC 6 cut(s) 375, 637, 946, 1485, 1489, 1557
BstDEI CTNAG 7 cut(s) 167, 300, 358, 378, 573, 823, 1674
BstF5I GGATG 5 cut(s) 39, 422, 1644, 1662, 1752
BstFNI CGCG 1 cut(s) 117
BstH2I RGCGCY 1 cut(s) 1382
BstHHI GCGC 4 cut(s) 760, 1327, 1381, 1756
BstKTI GATC 4 cut(s) 538, 718, 742, 1467
BstMAI GTCTC 3 cut(s) 335, 956, 1689
BstMBI GATC 4 cut(s) 535, 715, 739, 1464
BstNI CCWGG 5 cut(s) 332, 1278, 1305, 1376, 1394
BstNSI RCATGY 1 cut(s) 377
BstSCI CCNGG 6 cut(s) 208, 330, 1276, 1303, 1374, 1392
BstSLI GKGCMC 2 cut(s) 684, 783
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 8 cut(s) 175, 418, 553, 680, 712, 1087, 1462, 1593
BstV2I GAAGAC 1 cut(s) 656
BstX2I RGATCY 2 cut(s) 535, 715
BstYI RGATCY 2 cut(s) 535, 715
BsuRI GGCC 9 cut(s) 477, 750, 770, 885, 1010, 1031, 1197, 1262, 1281
BtgZI GCGATG 2 cut(s) 107, 412
BtsCI GGATG 5 cut(s) 39, 422, 1644, 1662, 1752
BtsI GCAGTG 1 cut(s) 1566
BtsIMutI CAGTG 3 cut(s) 61, 297, 1566
BveI ACCTGC 1 cut(s) 1066
Cac8I GCNNGC 6 cut(s) 375, 637, 946, 1485, 1489, 1557
CaiI CAGNNNCTG 1 cut(s) 1277
CfoI GCGC 4 cut(s) 760, 1327, 1381, 1756
Cfr13I GGNCC 3 cut(s) 409, 883, 1029
CspCI CAANNNNNGTGG 2 cut(s) 1210, 1245
CviAII CATG 7 cut(s) 374, 406, 473, 766, 1477, 1591, 1612
DdeI CTNAG 7 cut(s) 167, 300, 358, 378, 573, 823, 1674
DpnI GATC 4 cut(s) 537, 717, 741, 1466
DpnII GATC 4 cut(s) 535, 715, 739, 1464
EaeI YGGCCR 5 cut(s) 475, 768, 1008, 1260, 1279
Eam1104I CTCTTC 4 cut(s) 101, 131, 1388, 1499
EarI CTCTTC 4 cut(s) 101, 131, 1388, 1499
Eco130I CCWWGG 2 cut(s) 29, 1048
Eco24I GRGCYC 1 cut(s) 1541
Eco31I GGTCTC 2 cut(s) 335, 956
Eco47I GGWCC 1 cut(s) 409
Eco57I CTGAAG 2 cut(s) 266, 1100
Eco72I CACGTG 1 cut(s) 508
Eco88I CYCGRG 3 cut(s) 84, 246, 1546
EcoRI GAATTC 1 cut(s) 544
EcoRII CCWGG 5 cut(s) 330, 1276, 1303, 1374, 1392
EcoT14I CCWWGG 2 cut(s) 29, 1048
EcoT22I ATGCAT 1 cut(s) 862
EcoT38I GRGCYC 1 cut(s) 1541
ErhI CCWWGG 2 cut(s) 29, 1048
FaeI CATG 7 cut(s) 377, 409, 476, 769, 1480, 1594, 1615
FalI AAGNNNNNCTT 2 cut(s) 712, 744
FatI CATG 7 cut(s) 373, 405, 472, 765, 1476, 1590, 1611
FauI CCCGC 2 cut(s) 644, 1142
FbaI TGATCA 1 cut(s) 1464
FokI GGATG 4 cut(s) 46, 409, 1649, 1651
FriOI GRGCYC 1 cut(s) 1541
FspBI CTAG 2 cut(s) 930, 1484
GlaI GCGC 4 cut(s) 759, 1326, 1380, 1755
GsuI CTGGAG 2 cut(s) 821, 978
HaeII RGCGCY 1 cut(s) 1382
HaeIII GGCC 9 cut(s) 477, 750, 770, 885, 1010, 1031, 1197, 1262, 1281
HapII CCGG 2 cut(s) 209, 1666
HhaI GCGC 4 cut(s) 760, 1327, 1381, 1756
Hin1II CATG 7 cut(s) 377, 409, 476, 769, 1480, 1594, 1615
Hin6I GCGC 4 cut(s) 758, 1325, 1379, 1754
HinP1I GCGC 4 cut(s) 758, 1325, 1379, 1754
HinfI GANTC 7 cut(s) 203, 224, 267, 302, 614, 994, 1152
HpaII CCGG 2 cut(s) 209, 1666
HphI GGTGA 3 cut(s) 194, 198, 233
Hpy166II GTNNAC 1 cut(s) 185
Hpy188I TCNGA 5 cut(s) 147, 272, 285, 1521, 1714
Hpy188III TCNNGA 6 cut(s) 84, 482, 998, 1156, 1411, 1666
Hpy8I GTNNAC 1 cut(s) 185
HpyAV CCTTC 4 cut(s) 422, 769, 874, 1432
HpyCH4III ACNGT 7 cut(s) 75, 655, 835, 1090, 1249, 1273, 1573
HpyCH4IV ACGT 2 cut(s) 507, 1523
HpyCH4V TGCA 9 cut(s) 422, 505, 860, 865, 944, 980, 1061, 1166, 1568
HpyF3I CTNAG 7 cut(s) 167, 300, 358, 378, 573, 823, 1674
HpySE526I ACGT 2 cut(s) 507, 1523
Hsp92II CATG 7 cut(s) 377, 409, 476, 769, 1480, 1594, 1615
HspAI GCGC 4 cut(s) 758, 1325, 1379, 1754
Kpn2I TCCGGA 1 cut(s) 1665
Ksp22I TGATCA 1 cut(s) 1464
Kzo9I GATC 4 cut(s) 535, 715, 739, 1464
LguI GCTCTTC 1 cut(s) 1388
LmnI GCTCC 5 cut(s) 31, 89, 195, 1188, 1613
Lsp1109I GCAGC 8 cut(s) 175, 418, 553, 680, 712, 1087, 1462, 1593
LweI GCATC 2 cut(s) 431, 1718
MaeI CTAG 2 cut(s) 930, 1484
MaeII ACGT 2 cut(s) 507, 1523
MaeIII GTNAC 5 cut(s) 204, 221, 290, 932, 1094
MalI GATC 4 cut(s) 537, 717, 741, 1466
MboI GATC 4 cut(s) 535, 715, 739, 1464
MfeI CAATTG 1 cut(s) 306
MflI RGATCY 2 cut(s) 535, 715
MhlI GDGCHC 3 cut(s) 684, 783, 1541
MlsI TGGCCA 2 cut(s) 477, 1281
MluCI AATT 7 cut(s) 256, 276, 286, 306, 544, 1036, 1161
MluNI TGGCCA 2 cut(s) 477, 1281
MlyI GAGTC 7 cut(s) 212, 218, 276, 311, 608, 1003, 1161
Mox20I TGGCCA 2 cut(s) 477, 1281
Mph1103I ATGCAT 1 cut(s) 862
MroI TCCGGA 1 cut(s) 1665
MroXI GAANNNNTTC 1 cut(s) 279
MscI TGGCCA 2 cut(s) 477, 1281
MseI TTAA 5 cut(s) 585, 744, 939, 1016, 1759
MslI CAYNNNNRTG 2 cut(s) 770, 1589
Msp20I TGGCCA 2 cut(s) 477, 1281
MspA1I CMGCKG 3 cut(s) 166, 362, 1078
MspI CCGG 2 cut(s) 209, 1666
MspR9I CCNGG 6 cut(s) 210, 332, 1278, 1305, 1376, 1394
MunI CAATTG 1 cut(s) 306
MvaI CCWGG 5 cut(s) 332, 1278, 1305, 1376, 1394
MvnI CGCG 1 cut(s) 117
NciI CCSGG 1 cut(s) 210
NdeII GATC 4 cut(s) 535, 715, 739, 1464
NheI GCTAGC 1 cut(s) 1483
NlaIII CATG 7 cut(s) 377, 409, 476, 769, 1480, 1594, 1615
NlaIV GGNNCC 5 cut(s) 27, 717, 1030, 1147, 1609
NmuCI GTSAC 3 cut(s) 204, 221, 1094
NsiI ATGCAT 1 cut(s) 862
NspI RCATGY 1 cut(s) 377
PaeI GCATGC 1 cut(s) 377
PaeR7I CTCGAG 3 cut(s) 84, 246, 1546
PciSI GCTCTTC 1 cut(s) 1388
PdmI GAANNNNTTC 1 cut(s) 279
PleI GAGTC 7 cut(s) 211, 218, 275, 310, 608, 1002, 1160
PmaCI CACGTG 1 cut(s) 508
PmlI CACGTG 1 cut(s) 508
PpsI GAGTC 7 cut(s) 211, 218, 275, 310, 608, 1002, 1160
Ppu21I YACGTR 1 cut(s) 508
PshBI ATTAAT 2 cut(s) 585, 939
Psp6I CCWGG 5 cut(s) 330, 1276, 1303, 1374, 1392
PspCI CACGTG 1 cut(s) 508
PspGI CCWGG 5 cut(s) 330, 1276, 1303, 1374, 1392
PspN4I GGNNCC 5 cut(s) 27, 717, 1030, 1147, 1609
PspPI GGNCC 3 cut(s) 409, 883, 1029
PspXI VCTCGAGB 2 cut(s) 246, 1546
PsrI GAACNNNNNNTAC 2 cut(s) 1672, 1704
PstNI CAGNNNCTG 1 cut(s) 1277
PsuI RGATCY 2 cut(s) 535, 715
PvuII CAGCTG 2 cut(s) 166, 1078
RseI CAYNNNNRTG 2 cut(s) 770, 1589
SapI GCTCTTC 1 cut(s) 1388
SaqAI TTAA 5 cut(s) 585, 744, 939, 1016, 1759
Sau3AI GATC 4 cut(s) 535, 715, 739, 1464
Sau96I GGNCC 3 cut(s) 409, 883, 1029
SchI GAGTC 7 cut(s) 212, 218, 276, 311, 608, 1003, 1161
ScrFI CCNGG 6 cut(s) 210, 332, 1278, 1305, 1376, 1394
SduI GDGCHC 3 cut(s) 684, 783, 1541
SfaNI GCATC 2 cut(s) 431, 1718
Sfr274I CTCGAG 3 cut(s) 84, 246, 1546
SinI GGWCC 1 cut(s) 409
SlaI CTCGAG 3 cut(s) 84, 246, 1546
SmiMI CAYNNNNRTG 2 cut(s) 770, 1589
SmlI CTYRAG 4 cut(s) 84, 246, 482, 1546
SmoI CTYRAG 4 cut(s) 84, 246, 482, 1546
SphI GCATGC 1 cut(s) 377
Sse9I AATT 7 cut(s) 256, 276, 286, 306, 544, 1036, 1161
SspI AATATT 1 cut(s) 589
SspMI CTAG 2 cut(s) 930, 1484
StyD4I CCNGG 6 cut(s) 208, 330, 1276, 1303, 1374, 1392
StyI CCWWGG 2 cut(s) 29, 1048
TaaI ACNGT 7 cut(s) 75, 655, 835, 1090, 1249, 1273, 1573
TaiI ACGT 2 cut(s) 510, 1526
TaqI TCGA 7 cut(s) 66, 85, 247, 320, 542, 1502, 1547
TasI AATT 7 cut(s) 256, 276, 286, 306, 544, 1036, 1161
TauI GCSGC 4 cut(s) 245, 1197, 1262, 1346
Tru1I TTAA 5 cut(s) 585, 744, 939, 1016, 1759
Tru9I TTAA 5 cut(s) 585, 744, 939, 1016, 1759
TscAI CASTG 3 cut(s) 61, 304, 1566
TseFI GTSAC 3 cut(s) 204, 221, 1094
TseI GCWGC 8 cut(s) 163, 431, 566, 668, 725, 1075, 1450, 1581
Tsp45I GTSAC 3 cut(s) 204, 221, 1094
TspDTI ATGAA 5 cut(s) 341, 650, 1339, 1375, 1486
TspGWI ACGGA 1 cut(s) 202
TspRI CASTG 3 cut(s) 61, 304, 1566
VpaK11BI GGWCC 1 cut(s) 409
VspI ATTAAT 2 cut(s) 585, 939
XapI RAATTY 3 cut(s) 256, 276, 544
XceI RCATGY 1 cut(s) 377
XhoI CTCGAG 3 cut(s) 84, 246, 1546
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 2 cut(s) 930, 1484
Zsp2I ATGCAT 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.