pycom02g00010

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0006581
Physical Location & Seq
Reverse (-)
1380 .. 2169
790 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g00010.1

Sequence Viewer

Length: 438 bp
ATGTTGAAGCATATGGACCTTCAAACTGCATCAATTGCCGCCAGAAATGGCTACGATCCATTCCACATTGCTGTAAGACAGGGCCATCTTGAGGTGTTGAAAGAACTTCTGCACGTGTTCCCGAACTTGGCTATGACCACAGATTTATCCAATTCAACTGCCTTACACACGGCTGCTACTCAGGGGCATATTGATATTGTAAATCTCCTTTTGGAAACTGACTCAAATCTCGCCAAGATTGCCCGCAATAATGGTAAGACTGTGCTTCATTCAGCAGCAAGGATGGGGCACTTGGAAGTAGTCAAGTCCCTACTAAGAAAGGATCCAAGTGCTGCTTTTAGAACTGACCTGAAAGGCCAAACTGCGTTGCACATGGCTGTAAAAGGGCACAATGAGGAGATTGTGCTGGAGTTGCTGAAACCCGACCCTCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005886 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 PGG

Protein Analysis

146

Amino Acids

15.97

Weight (kDa)

7.18

Isoelectric Point (pI)

25.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 10 - 75 9.3e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 22 - 71 4.7e-08 Ankyrin repeats (many copies)
Ank_4 PF13637 55 - 104 1.4e-10 Ankyrin repeats (many copies)
Ank_2 PF12796 82 - 141 1.7e-12 Ankyrin repeats (3 copies)
Ank PF00023 84 - 109 6.6e-06 Ankyrin repeat
Ank_4 PF13637 89 - 135 3.7e-08 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 39, 244
AclWI GGATC 3 cut(s) 50, 317, 330
AcvI CACGTG 1 cut(s) 115
AfiI CCNNNNNNNGG 2 cut(s) 91, 127
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 4 cut(s) 7, 23, 100, 156
AlwI GGATC 3 cut(s) 50, 317, 330
AoxI GGCC 2 cut(s) 82, 355
ApeKI GCWGC 3 cut(s) 173, 275, 332
AspS9I GGNCC 2 cut(s) 16, 82
AvaII GGWCC 1 cut(s) 16
BaeGI GKGCMC 2 cut(s) 291, 390
BamHI GGATCC 1 cut(s) 322
BbrPI CACGTG 1 cut(s) 115
BbvI GCAGC 3 cut(s) 160, 287, 319
BccI CCATC 2 cut(s) 93, 277
BceAI ACGGC 1 cut(s) 186
BisI GCNGC 4 cut(s) 39, 174, 276, 333
BlsI GCNGC 4 cut(s) 40, 175, 277, 334
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 2 cut(s) 16, 82
BmiI GGNNCC 1 cut(s) 324
BmsI GCATC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 110
BsaAI YACGTR 1 cut(s) 115
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 127
Bse3DI GCAATG 1 cut(s) 66
BseGI GGATG 1 cut(s) 288
BseLI CCNNNNNNNGG 2 cut(s) 91, 127
BseMI GCAATG 1 cut(s) 66
BseMII CTCAG 1 cut(s) 194
BseRI GAGGAG 1 cut(s) 410
BseSI GKGCMC 2 cut(s) 291, 390
BseXI GCAGC 3 cut(s) 160, 287, 319
BsgI GTGCAG 1 cut(s) 95
BshFI GGCC 2 cut(s) 84, 357
BslFI GGGAC 1 cut(s) 292
BslI CCNNNNNNNGG 2 cut(s) 91, 127
BsmFI GGGAC 1 cut(s) 292
BsnI GGCC 2 cut(s) 84, 357
Bsp1286I GDGCHC 2 cut(s) 291, 390
Bsp143I GATC 2 cut(s) 55, 322
BspACI CCGC 2 cut(s) 39, 244
BspANI GGCC 2 cut(s) 84, 357
BspCNI CTCAG 1 cut(s) 193
BspLI GGNNCC 1 cut(s) 324
BspPI GGATC 3 cut(s) 50, 317, 330
BsrDI GCAATG 1 cut(s) 66
BssMI GATC 2 cut(s) 55, 322
Bst4CI ACNGT 1 cut(s) 262
BstAPI GCANNNNNTGC 1 cut(s) 35
BstBAI YACGTR 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 244
BstDEI CTNAG 3 cut(s) 180, 314, 429
BstF5I GGATG 1 cut(s) 288
BstKTI GATC 2 cut(s) 58, 325
BstMBI GATC 2 cut(s) 55, 322
BstMWI GCNNNNNNNGC 3 cut(s) 35, 239, 412
BstSLI GKGCMC 2 cut(s) 291, 390
BstV1I GCAGC 3 cut(s) 160, 287, 319
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BsuRI GGCC 2 cut(s) 84, 357
BtsCI GGATG 1 cut(s) 288
Cac8I GCNNGC 1 cut(s) 244
Cfr13I GGNCC 2 cut(s) 16, 82
CviAII CATG 1 cut(s) 373
CviJI RGCY 6 cut(s) 51, 84, 131, 173, 357, 377
CviKI_1 RGCY 6 cut(s) 51, 84, 131, 173, 357, 377
DdeI CTNAG 3 cut(s) 180, 314, 429
DpnI GATC 2 cut(s) 57, 324
DpnII GATC 2 cut(s) 55, 322
Eco47I GGWCC 1 cut(s) 16
Eco72I CACGTG 1 cut(s) 115
FaeI CATG 1 cut(s) 376
FaiI YATR 5 cut(s) 12, 14, 134, 189, 374
FalI AAGNNNNNCTT 2 cut(s) 319, 351
FaqI GGGAC 1 cut(s) 292
FatI CATG 1 cut(s) 372
FauI CCCGC 1 cut(s) 251
FauNDI CATATG 1 cut(s) 12
Fnu4HI GCNGC 4 cut(s) 39, 174, 276, 333
FokI GGATG 1 cut(s) 295
Fsp4HI GCNGC 4 cut(s) 39, 174, 276, 333
GluI GCNGC 4 cut(s) 39, 174, 276, 333
GsuI CTGGAG 1 cut(s) 428
HaeIII GGCC 2 cut(s) 84, 357
Hin1II CATG 1 cut(s) 376
HinfI GANTC 1 cut(s) 221
Hpy188III TCNNGA 2 cut(s) 89, 121
HpyAV CCTTC 1 cut(s) 29
HpyCH4III ACNGT 1 cut(s) 262
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 3 cut(s) 29, 112, 370
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 239, 412
HpyF3I CTNAG 3 cut(s) 180, 314, 429
HpySE526I ACGT 1 cut(s) 114
Hsp92II CATG 1 cut(s) 376
Kzo9I GATC 2 cut(s) 55, 322
LpnPI CCDG 5 cut(s) 55, 65, 167, 362, 392
Lsp1109I GCAGC 3 cut(s) 160, 287, 319
LweI GCATC 1 cut(s) 38
MaeII ACGT 1 cut(s) 114
MalI GATC 2 cut(s) 57, 324
MboI GATC 2 cut(s) 55, 322
MfeI CAATTG 1 cut(s) 33
MflI RGATCY 1 cut(s) 322
MhlI GDGCHC 2 cut(s) 291, 390
MluCI AATT 2 cut(s) 33, 151
MlyI GAGTC 1 cut(s) 215
MnlI CCTC 3 cut(s) 85, 388, 438
MunI CAATTG 1 cut(s) 33
MwoI GCNNNNNNNGC 3 cut(s) 35, 239, 412
NdeI CATATG 1 cut(s) 12
NdeII GATC 2 cut(s) 55, 322
NlaIII CATG 1 cut(s) 376
NlaIV GGNNCC 1 cut(s) 324
PkrI GCNGC 4 cut(s) 40, 175, 277, 334
PleI GAGTC 1 cut(s) 215
PmaCI CACGTG 1 cut(s) 115
PmlI CACGTG 1 cut(s) 115
PpsI GAGTC 1 cut(s) 215
Ppu21I YACGTR 1 cut(s) 115
PspCI CACGTG 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 2 cut(s) 16, 82
PsuI RGATCY 1 cut(s) 322
SatI GCNGC 4 cut(s) 39, 174, 276, 333
Sau3AI GATC 2 cut(s) 55, 322
Sau96I GGNCC 2 cut(s) 16, 82
SchI GAGTC 1 cut(s) 215
SduI GDGCHC 2 cut(s) 291, 390
SetI ASST 4 cut(s) 21, 96, 117, 351
SfaNI GCATC 1 cut(s) 38
SinI GGWCC 1 cut(s) 16
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
Sse9I AATT 2 cut(s) 33, 151
SsiI CCGC 2 cut(s) 39, 244
TaaI ACNGT 1 cut(s) 262
TaiI ACGT 1 cut(s) 117
TasI AATT 2 cut(s) 33, 151
TauI GCSGC 1 cut(s) 41
TseI GCWGC 3 cut(s) 173, 275, 332
TspDTI ATGAA 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.